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HPLC
Rezex
Description
Applications
Additional Notes
RCM-Monosaccharide
(L19 packing)*
8 % cross-linked resin
CALCIUM ionic form
RHM-Monosaccharide
(L17 packing)*
8 % cross-linked resin
HYDROGEN ionic form
RAM-Carbohydrate
8 % cross-linked resin
SILVER ionic form
RSO-Oligosaccharide
4 % cross-linked resin
SILVER ionic form
RNO-Oligosaccharide
4 % cross-linked resin
SODIUM ionic form
RPM-Monosaccharide
(L34 packing)*
8 % cross-linked resin
LEAD ionic form
RNM-Carbohydrate
(L54 packing)*
8 % cross-linked resin
SODIUM ionic form
ROA-Organic Acid
(L22 packing)*
8 % cross-linked resin
HYDROGEN ionic form
RFQ-Fast Acid
8 % cross-linked resin
HYDROGEN ionic form
RKP-Potassium
8 % cross-linked resin
POTASSIUM ionic form
Analysis of glyphosate
8 % cross-linked resin
CALCIUM ionic form
210
Phenomenex
Organic Acids
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
45
1 3
App ID 5504
App ID 5515
REZEX
6
Saccharides
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
Rezex RCM-Monosaccharide
300 x 7.8 mm
00H-0130-K0
Water
0.6 mL/min
Rl
85 C
1. Melezitose
2. Maltose
3. Glucose
4. Mannose
5. Fructose
6. Ribitol
2 3
6
4 5
HPLC
12
24 min
16 min
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
Rezex RCM-Monosaccharide
300 x 7.8 mm
00H-0130-K0
Water
0.6 mL/min
RI
75 C
1. Sucrose
2. Glucose
3. Fructose
3
1
12
24
Oligosaccharides
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
Rezex RSO-Oligosaccharide
200 x 10 mm
00P-0133-N0
Water
0.3 mL/min
Rl
75 C
Malto-Oligosaccharides as shown
Dp2
Dp3
Dp4
Dp10
Dp11
Dp12
Dp13
Dp14
Carbohydrates
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
Rezex RKP-Potassium
300 x 7.8 mm
00H-3252-K0
Water
0.4 mL/min
RI
85 C
1. Maltotriose
2. Maltose
3. Glucose
36 min
App ID 5508
App ID 5507
App ID 5506
App ID 5505
Rezex
Apple Juice
10
20 min
Saccharides
Column:
Rezex RPM-Monosaccharide
Dimensions:
300 x 7.8 mm
00H-0135-K0
Part No.:
Mobile Phase: Water
0.6 mL/min
Flow Rate:
Detection:
RI
Temperature: 75 C
Sample:
1. Stachyose
2. Maltose
3. Glucose
2
4. Xylose
5. Galactose
6. Fructose
1
7. Meso-Erythritol
8. Mannitol
9. Salicin
10. Xylitol
11. Sorbitol
3 5
4
6
7
8
10
9
11
Dp5
Dp9
Dp8 Dp6
Dp7
Dp1
35
min
10
20
30
40 min
Phenomenex
211
Food Softeners
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
App ID 5510
App ID 5518
REZEX
Rezex RCM-Monosaccharide
300 x 7.8 mm
00H-0130-K0
Water
0.5 mL/min
RI
60 C
1. Glycerol
2. Methoxypolyethylene Glycol
3. Triethylene Glycol
4. Sorbitol
5. Urea
Carbohydrates
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
Rezex RNM-Carbohydrate
300 x 7.8 mm
00H-0136-K0
Water
0.6 mL/min
RI
75 C
1. Stachyose
5. Arabinose
2. Cellobiose
6. Ribose
3. Glucose
7. Salicin (1 % solution of each)
4. Fructose
1
2
3
HPLC
4
5
2
5
Rezex
10.00
20.00
2
3
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
Rezex RCM-Monosaccharide
300 x 7.8 mm
00H-0130-K0
Water
0.5 mL/min
ELSD
80 C
1. Sucrose
2. Glucose
3. Galactose
4. Fructose
Rezex RPM-Monosaccharide
100 x 7.8 mm
00D-0135-K0
Water
0.6 mL/min
ELSD
80 C
1. Mannitol
2. Sorbitol
16
24 min
10 min
MIN
Amino Sugars
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
23
2
4
212
25 min
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
Fermentation Broth
App ID 15501
Carboxylic Acids
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
30.00 min
App ID 5514
App ID 5517
0.00
4
10 min
10 min
10
20
min
Phenomenex
REZEX
Rezex vs. Bio-Rad Aminex
Phenomenex guarantees satisfaction when using Rezex HPLC columns. As
illustrated below, Rezex offers advantages that enhance chromatographic
results, increase throughput, and simplify quantitation.
Rezex RCM-Monosaccharide
Aminex HPX-87C
300 x 7.8 mm
00H-0130-K0
Water
0.6 mL/min
ELSD
80 C
1. Melezitose
4. Mannose
2. Maltose
5. Fructose
3. Glucose
6. Ribitol
120
1
mAU
100
80
400
300
200
60
10
80
2
3
10
400
60
12
14
Aminex
HPX-87C
200
40
0
16 min
Aminex
HPX-87C
Difficult Quantitation
mAU
100
20 min
6
100
0
10
20 min
10
12
14
16 min
Aliphatic Acids
Sugars
100
mAU
Rezex
RHM
120
2
80
60
Rezex
20
32
Aminex
10
120
20 min
100
80
60
40
Aminex
HPX-87H
20
55
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
Rezex RCM-Monosaccharide
Aminex HPX-87C
300 x 7.8 mm
00H-0130-K0
Water
0.6 mL/min
ELSD
80 C
1. Sucrose
4. Fructose
2. Glucose
5. Sorbitol
3. Galactose
Rezex
RCM
Baseline
Resolution
1
80
Bar Bar
40
mAU
Rezex RHM-Monosaccharide
Aminex HPX-87H
300 x 7.8 mm
00H-0132-K0
0.005N H2SO4
0.5 mL/min
UV @ 210 nm
40 C
1. Oxalic Acid
4. Succinic Acid
2. Citric Acid
5. Formic Acid
3. Tartaric Acid
6. Acetic Acid
mAU
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
60
40
10
20
30 min
100
80
mAU
Rezex
RCM
500
40
mAU
Rezex RCM-Monosaccharide
Aminex HPX-87C
300 x 7.8 mm
00H-0130-K0
Water
0.6 mL/min
ELSD
80 C
1. Sucrose
2. Glucose
3. Fructose
Rezex
RCM
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
mAU
Superior
Peak Shape
Orange Juice
Rezex
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
HPLC
Saccharides
60
2
40
Aminex
HPX-87C
0
0
Phenomenex
10
20 min
10
20
30 min
213
REZEX
Specifications and Operating Recommendations
Rezex
HPLC
RCM Monosaccharide
RSO Oligosaccharide
RNO Oligosaccharide
RNM Carbohydrate
RPM Monosaccharide
RHM Monosaccharide
214
RAM Carbohydrate
00H-0131-K0
Silver
300 x 7.8 mm
8%
8 m
35,000
350
600
0.6
85
Water
Neutral
03B-0131-K0
2 % Silver Nitrate
>5 % Methanol,
Acids, Bases,
Non-Silver Salts/
Metal Ions
100 % Water
0.4
75
12
0.1M AgNO3
0.2
85
4-16
Water
Phenomenex
REZEX
Retention Times for Some Carbohydrates and Sugar
Alcohols
Conditions:
Counter Ion Analyte I RAM Ag+ I RCM Ca+2 I RNM Na+ I RHM H+ I RPM Pb+2
14.93 11.10 11.11
12.71 11.45 10.21
13.56 12.65 11.24
8.60 8.49
8.02
13.82 11.39 12.59
21.61 11.10 10.71
15.49 11.78 12.14
13.65 11.76 10.31
13.19 12.30 11.65
11.73 11.47 10.19
8.40
8.40
7.87
10.37 10.71
9.62
13.35 12.14
9.98
8.74
8.76
8.02
9.03
8.78
8.32
10.32
9.23
8.57
14.06 11.98 10.68
8.81 8.75 8.18
8.10
7.94
7.51
9.47 8.82 8.27
17.82 10.80 10.59
12.04 11.54 10.16
9.04
8.82 8.14
7.93
7.66
7.54*
8.16
7.76 7.88*
12.18 11.00 10.90
23.38 14.34 11.42
18.58 17.36 14.98
22.45 11.39 10.83
7.59
7.30 7.27
8.71
8.65 9.24*
8.72
8.49 8.32
22.01 12.16 11.78
11.62 11.68 10.24
20.15
15.82
16.47
11.00
15.32
33.25
19.82
17.71
16.19
14.94
10.53
12.92
18.87
11.28
11.89
13.95
16.66
11.59
11.02
12.40
24.90
16.39
11.97
9.94
10.28
14.47
33.48
26.81
35.97
9.72
11.00
11.01
32.38
13.84
ORDERING INFORMATION
Columns
Description
RCM-Monosaccharide
300 x 7.8 mm
Water (degassed)
0.6 mL/min
80 C
RI @ 40 C
Bio-Rad
Aminex
Supelco
Supelcogel
Waters
Sugar-Pak
RCM-Monosaccharide
HPX-87C
125-0095
Supelcogel Ca
Sugar-Pak 1
RHM-Monosaccharide
HPX-87H
125-0140
N/A
RPM-Monosaccaride
HPX-87P
125-0098
Supelcogel Pb
N/A
RNM-Carbohydrate
HPX-87N
125-0143
N/A
N/A
RSO-Oligosaccharide
HPX-42A
125-0097
N/A
ROA-Organic Acid
HPX-87H
125-0140
N/A
RFQ-Fast Acid
Fast Acid
125-0100
N/A
N/A
RKP-Potassium
HPX-87K
125-0142
Supelcogel K
N/A
Sugar Alcohols
125-0094
N/A
N/A
Rezex
11.54
11.95
13.01
8.86
11.90
11.64
12.31
12.05
12.75
11.87
8.70
11.04
12.59
9.11
9.27
9.75
12.41
9.16
8.27
9.25
11.36
12.04
9.26
8.00
8.10
11.50
14.59
18.51
11.91
7.60
9.03
8.91
12.69
12.06
Dimensions:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
HPLC
Adonitol (Ribitol)
D-Altrose
D-(-)-Arabinose
D-(+)-Cellobiose
D-(+)-Digitoxose
Dulcitol
Meso-Erythritol
D-(-)- Fructose
L-(-)-Fucose
D-(+)-Galactose
Gentiobiose
D-(+)-Glucose
Inositol
Isomaltose
Lactose
Lactulose
D- Lyxose
D- Maltose
Maltotriose
Maltulose
D- Mannitol
D-(+)-Mannose
Melibiose
D-(+)-Melezitose
D-(+)-Raffinose
L-(+)-Rhamnose
D-(-)-Ribose
Salicin
D-Sorbitol
Stachyose
Sucrose
Trehalose
Xylitol
D-(+)-Xylose
Cross Ionic
Part No.
Linkage Form
I 00H-0130-K0 I 8 %
I Calcium
Size (mm) Price
I 300 x 7.8 I
Guards
Part No.
SecurityGuardCartridges
4 x 3.0 mm
Size (mm) Price
/8pk
I
03B-0130-K0 I 50 x 7.8
AJ0-4493
03B-0132-K0 50 x 7.8
AJ0-4490
AJ0-4491
03R-0133-N0 60 x 10.0
AJ0-4491
03R-0137-N0 60 x 10.0
03B-0135-K0 50 x 7.8
03B-0135-K0 50 x 7.8
AJ0-4492
AJ0-4492
03B-0136-K0 50 x 7.8
03B-0138-K0 50 x 7.8
AJ0-4490
03B-0223-K0 50 x 7.8
03A-0130-D0 30 x 4.0
AJ0-4490
AJ0-4493
for ID: 3.2-8.0 mm
Phenomenex
215