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Optical Density
0.20
0.15
0.10
Unknown 1
Unknown 2
Unknown 3
0.05
0.00
X
2.307
2.607
3.857
micrograms
(Interpolated)
Upper Limit
2.427
2.719
3.952
Lower Limit
2.180
2.489
3.762
Optical Density
(Entered)
Y1
0.067
0.073
0.098
micrograms
If you didnt pick the sample data set, enter data for the standard curve (shown below). In the sample data,
rows 1-7 are for the standard curve, where you know both X and Y. The Y column of rows 8-10 has the optical
density of the unknown samples. The corresponding X values are intentionally left blank. Those are the values
we need to figure out.
Click twice on the default sheet name in the Prism Navigator tree to rename the sheet.
View the graph that is produced from the data by clicking Protein Standard Curve under Graphs in the
navigator bar. In the Change Graph Type dialog box that appears, select Points only (highlighted by the red
arrow):
Optical Density
0.20
0.15
0.10
0.05
0.00
micrograms
Note: This Interpolate analysis is new to Prism 6. You could also do the interpolation using the
linear or nonlinear regression analyses, but this new Interpolate analysis is more focused and
thus easier to use.
In the Parameters: Interpolate a Standard Curve dialog box, select Line. Uncheck the box next to Plot
curve with 95% confidence band. These selections are highlighted below with red arrows.
Optical Density
0.20
0.15
0.10
0.05
0.00
micrograms
Prism performs the fit and displays the outcome in sheets under the Results heading. The Interpolated X
values sheet listed under this analysis of the Results to view the unknown values computed based on the
linear regression. Prism reports the corresponding X value for each unpaired Y value on the data sheet.
Switch to the Table of results sheet (shown below). This sheet provides the best-fit values from the analysis:
After you have made these changes, the graph includes the unknown values:
Protein standard curve
Optical Density
0.20
Optical Density
(Entered)
0.15
0.10
0.05
0.00
micrograms
Optical Density
0.20
Optical Density
(Entered)
0.15
0.10
0.05
0.00
micrograms
Optical Density
0.20
0.15
0.10
Unknown 1
Unknown 2
Unknown 3
0.05
0.00
X
2.307
2.607
3.857
micrograms
(Interpolated)
Upper Limit
2.427
2.719
3.952
Lower Limit
2.180
2.489
3.762
Optical Density
(Entered)
Y1
0.067
0.073
0.098
micrograms