Objectives To Get To The Purpose

Stacking Traits.Along with Profit.

The project has been approved on Jan 15, 2013, and was initiated the same day. From that time forward, we have received, all agreed funds (not all) towards purchase of the intended equipment, consumables related to
project. The Progress report is due for the period of 31-36th month period. According to agreed objectives, we have met the required targets, and we are proceeding with great strength and full speed towards achieving the
intended goal.

1.
2.
1.

7-12
2.
1.
2.
13-18

3.
4.
1.

19-24

2.
1.

25-30

2.
1.

31-36

2.
1.

37-42

43-48
49-54

2.
1.
1.
1.

55-60
2.

Phenotypic identification of the diverse maize germplasm for carotenoid content

Donor plants carrying opaque 2 will be crossed with our NSL elite lines to generate F1
progeny
Identification of donors with superior levels of carotenoids, to use in the crossing program
and transfer superior carotenoid alleles into elite lines.
F1 progeny for QPM will be backcrossed with elite recurrent parent to generate BC1F1
Standard PCR based cloning techniques, genome walking, sequencing, and data analysis
using bio-informatics tools
BC1F1 will be self-pollinated to produce BC1F2 and the same will undergo for foreground
selection for opaque 2 using gene specific markers
Lines carrying opaque 2 will be screened for background selection using recurrent parent
specific SSR markers.
Donor plants carrying superior carotenoid alleles will be crossed with elite lines to
generate F1 progeny
F1 progeny carrying superior carotenoid alleles will be backcrossed with elite recurrent
parent to generate BC1F1, while performing biochemical and molecular analysis.
BC1F2 carrying opaque2 will be self-pollinated to generate BC1F3 and will undergo for
foreground and background selection
BC1F1 carrying superior carotenoid alleles will be self-pollinated, to generate BC1F2, while
performing biochemical and molecular analysis.
BC1F3 carrying opaque2 will be self-pollinated to generate BC1F4 and will undergo for
foreground and background selection
BC1F4 carrying opaque2 will be self-pollinated to generate BC1F5 and will undergo for
foreground and background selection
BC1F2 carrying superior carotenoid alleles will be self-pollinated, to generate BC1F3, while
performing biochemical and molecular analysis.
BC1F5 carrying opaque2 will be self-pollinated to generate BC1F6 (QPM Fixed elite line)
and will undergo for foreground and background selection
BC1F3 carrying superior carotenoid alleles will be self-pollinated, to generate BC1F4, while
performing biochemical and molecular analysis.
Fixed QPM elite line will be crossed with BC1F4, carrying superior carotenoid alleles, to
generate F1 carrying both traits of interest.
F1 progeny will be self-pollinated to generate F2
F2 population seed will be subjected to both morphological, biochemical, and molecular
analysis for both traits of interest.
Selected lines will be further self-pollinated and continued to fix the traits.

Current
Status

In
Process

In
Process

Commercial Venture and Social

Corporate responsib
sibility

0-6

Achievable Targets
(Physical and Technical)

Unique Selling Point (USP)

Period of
Study
(In Months)

Back Cross scheme, using conventional

al and MAS to convert normal to QPM lines

Genetic component
o2

Genetic System
The recessive mutant allele of the o2 gene

Conventional breeding
Phenotypic observation in F2

Molecular breeding
Using SSRs with in o2 in
BC and F2

Current Status
Standardized the method is
currently in use

Hardness modifiers

The endosperm hardness modifier genes/QTL

Through light box screening

Markers???

Standardized the way and is

currently in use

Amino acid modifiers

The amino acid modifier genes/QTL influencing

amino acid content in the endosperm

Through estimation of Lysine and

Tryptophan

Markers???

In progress

SELFING
SELFING

BC1F4
Population

BC1F3
Population
SELFING

Elite
Line

F1
Progeny

BC1F1
Population

BC1F2
Population

Select agronomically
and biochemically
superior BC2F3 line

Foreground selection
Phenotypic selection for improved
agronomic characters
Phenotypic selection for kernel
modification and tryptophan conc.

QPM version
of elite line

Foreground selection
background selection of recurrent parent
Identification of individuals with highest
recurrent genome content

Foreground selection and limited

background selection
Determination of optimum population size
for BC2

Line

Foreground Selection of BC1F2 progenies for opaque2 allele

P1 P2 O O O O H H
N H N O H N N N

O
N

P1 P2 O H H H O H
H O H H H H H O

H H

P1 P2 H
H O H

P1 P2 H
N N H

H
H

H
O

H
O

H
N

H
H

N
N

O
N

N
O

N
N

P1 P2 O N O N N N N
O H O N N H H O

P1 P2 N
H H O

H
H

H
O

O
H

H
O

H
H

P1 P2 H
H H N

H
H

H
O

O O
O O

H H

Elite parents selected for the

work
1 O2o2 [Hetero] + Elite type
2
o2o2only
3
O2o2 [Hetero] only
4
Normal

3001

3003

M183

46
24
66
40

40
35
20
45

22
11
18
50

Hardness of endosperm - Scoring of Endosper

erm modification through conventional light box

IV

A212

III

II

B114

Type (Modification Score) I:

Type (Modification Score) II:
Type (Modification Score) III:
Type (Modification Score) IV:
Type (Modification Score) V:

G270

Not opaque
25% opaque
50% opaque
75% opaque
100% opaque

Estimation of Tryptophan in maize donor parent seed and recurrent parent seed

P
BC1F3 (Bulk)

Donors

BC1F3 (Bulk)

Elite Cultures

A
1.0
bnlg1179

1.01
bnlg1066

bnlg1007
1.02

2.0
bnlg1092

2.01

3.0
phi049

bnlg1203

umc2148
1.03

bnlg105

5.02

bnlg2248

3.03

4.03

2.04

1.07

2.05

4.04
mmc0321

bnlg1036

5.04

bnlg1137

4.05

bnlg1237

3.05

bnlg2242

dupssr34

bnlg1605

3.07

bnlg1444

4.08

8.02

bnlg1270

7.03

10.04
9.05

bnlg1146

8.03

bnlg2190
bnlg1031

8.04

bnlg345

8.05

4.09
dupssr28

bnlg1740

bnlg1056

6.06
phi116

9.08

bnlg1480

10.07

10

7.05

bnlg2244

10.06
9.07

bnlg1588

bnlg1828

3.08

bnlg1129

phi057

5.09

mmc0381

10.05
9.06

7.04
6.05

bnlg1185

umc2346

umc1799

bnlg2249

bnlg1711

10.03

bnlg1176

6.04

bnlg1746
2.09

bnlg1688
9.04

bnlg1154

5.08

umc1148

8.06

6.07

phi120

2.10

bnlg1182

4.10

phi1099
umc1605

bnlg1666

bnlg1306

bnlg1267

8.01
umc1415
umc1001

5.07

4.07

dupssr23

1.11

6.02

umc1077

mmc0481

2.08

umc1737

umc1083

bnlg1617
3.06

1.09

1.10

5.06

bnlg505

bnlg2144

bnlg1502

bnlg1055

5.05

6.03
mmc0282

10.02
9.03
umc1863

bnlg2077
2.07

umc1191

bnlg2289
7.02
bnlg1070

4.06
bnlg2291

1.08

bnlg1655

umc2316
bnlg2136

10.01

bnlg1200

bnlg1432

bnlg2323

2.06

bnlg1629

umc1802

6.01

dupssr19

bnlg2235

dupssr18
bnlg1904

mmc0501
9.02

7.01

bnlg1265
bnlg1113

bnlg1401

10.0
8.0

bnlg2132

bnlg1046
bnlg1902

3.04
mmc0231

bnlg1451

bnlg1194

bnlg1741

phi037

1.12

1
G

bnlg1043
bnlg1600

bnlg1175

bnlg1288
7.0

5.03

2.03

bnlg1887

bnlg1642

bnlg1162
6.0

bnlg1447

1.06

9.01

bnlg1879

bnlg1325

umc1035

umc1335

B
B

bnlg1272
4.02

3.02
1.05

9.0

bnlg1126

mmc0111

umc2083

bnlg1144

5.01

4.01

3.01
2.02

1.04

4.0

bnlg1017

bnlg1811

5.0

mmc0121

7.06

umc1834
3.09

4.11

umc2165
6.08

dupssr14

8.07
8.08
8.09

8
Total Markers screened:200
Best Markers: 77

77 are being used to recover the

recurrent genome

Plant
No.
27
5
10
1
26
14
22
13
19
11
16
23
2
17
28
24
18
15

%of genome
Elite QPM Het.
42.7 25.2 32.1
50.7 22.9 26.4
52.1 20.8 27.1
63.6 20.7 15.7
57.0 18.3 24.6
58.1 18.2 23.6
55.8 15.9 28.3
54.5 14.5 31.0
57.9 14.5 27.6
63.1 14.1 22.8
73.3 10.4 16.3
60.2 26.6 13.3
61.6 13.7 24.7
62.1 13.1 24.8
58.7 13.0 28.3
54.7 12.8 32.4
62.2 10.4 27.4
67.2 10.2 22.6

ELITE Plants in LOT-B

%of genome
Elite QPM Het.
70.0 9.4 20.6
61.0 15.7 23.3
58.6 16.0 25.3
58.4 13.7 28.0
57.6 18.8 23.6
43.6 13.3 43.0
53.0 16.7 30.4
75.4 15.0 9.6
74.6 10.1 15.4
64.3 18.5 17.3
57.7 20.8 21.4
54.2 16.7 29.2
53.6 12.7 33.7

Plant
%of genome
No. Elite QPM Het.
17
17
70
14
1
14
68
18
10
18
67
17
23
61
26
17
7
53
23
30
16
46
21
35
3
59
20
30
5
52
19
36
9
48
18
36
6
46
33
26
28
50
32
26
2
41
30
35
15
55
28
26
12
44
24
39
13
45
24
33
14
55
21
33

Plant
%of genome
No. Elite QPM Het.
27
73
11
25
4
63
18
27
8
60
12
36
3
59
20
30
18
57
14
29
7
53
23
30
19
54
15
40
24
57
13
26
21
46
15
41
29
58
12
31
23
61
26
17
22
61
18
28
15
55
28
26
11
55
18
35
14
55
21
23
5
52
19
36

ELITE Plants in LOT-F

Plant
No.
1
5
12
13
9
14
17
10
19
18
4
3
21

QPM Plants in LOT-B

%of genome
Elite QPM Het.
37.7 23.4 38.9
54.2 16.7 29.2
57.7 20.8 21.4
47.7 20.1 32.2
42.3 23.3 34.4
57.6 18.8 23.6
58.6 16 25.3
45 25.4 29.6
50 18.7 31.3
53 16.7 30.4
64.3 18.5 17.3
50.6 16.3 33.1

Plant
%of genome
No. Elite QPM Het.
30
72
10
17
23
72
15
13
17
70
14
16
12
67 15.4 17.6
27
63
20
17
2
62.3 12 25.7
26
74
10
16
28
73
12
16
22
71
13
17
31
70
7.9
22
5
69
12
19
19
69
15
16
8
68
14
19
33
67
17
16
15
63
13
24
16
63
14
23

QPM Plants in LOT-F

Plant
No.
2
3
4
7
8
9
12
15
16
17
18
20

ELITE Plants in LOT-A

%of genome
Elite QPM Het.
60
27
14
52
26
22
54
24
24
61
23
16
56.6 21.1 22.3
57
18
24
54
17
29
58.1 16.2 25.7
33.7 28.9 37.4
57
24
18
56
21
23
58
21
21
63
20
17
67
17
16
54.3 17.2 28.5
54.95 17.03 17.02

ELITE Plants in LOT-G

QPM Plants in LOT-A

Plant
No.
4
24
11
6
1
9
32
13
25
21
20
10
27
33
18
3

QPM Plants in LOT-G

Selection of Best Elite and QPM counterparts for [F1] Hybridization program (pre-commercial
(pre
activity)

Plant
No.
1
2
3
6
7
9
11
12
15
16
17
18
20
21
23
25
28
29

%of genome
Elite QPM Het.
63.6 20.7 15.7
61.6 13.7 24.7
67.4 5.9 26.7
58.1 10.1 31.8
68.7 6.8 24.5
65.5 9.5 25.0
63.1 14.1 22.8
58.8 8.8 32.4
67.2 10.2 22.6
73.3 10.4 16.3
62.1 13.1 24.8
62.2 10.4 27.4
71.2 9.4 19.4
78.8 6.8 14.4
60.2 26.6 13.3
67.1 7.7 25.2
58.7 13.0 28.3
66.7 8.3 25.0

Cross Hybridization program (pre-commercial

commercial activity) keeping quality and quantity in harmony

LOT G QPM LINES

LOT A QPM LINES

1
3

10 14 18 32

11 12 17 22 23 26 31 33

20

21

23

X
X

II
LOT F Lines

X
X

II

12

II
X

X
X
X
X
X

13

X
X
X

X
X
X

28

10 10 11 11 12 13 13 13 14 15 16 16 17 17 18 19 19 21 21

X
X

X
X
X
X

X
X
X
X

X
X
X
X

X
X

X
X
X
X

II
X

X
X

X
X

II

X
X
II

15

X
X

16

X
X
X
X

X
X
II
X
X
X
X

X
X
X
X

X
X
II
X
X

X
X
X
II
II

X
X
X
X
II
X
X

X
X
X
X

X
X

19
20
21
22
23

X
X

26
27
28
29

Selected for Elite line based on genome recovery data

Selected for QPM line based on genomerecovery data
selected for both Elite & QPM nature based on genome recovery data

X
X
X

X
X
X
X
X
X

X
X
X
X
X
X
X
X

X
X
X
X
X

X
X
X

X
X

X
X

X
X

X
X
X

X
X

X
X
X

X
X

X
X

X
X

X
X
X

X
X

X
X

X
X

X
X
X
X

X
X
X
X

X
X

18

II
X
X
X

X
X
X
X
X

17

25

14

25

13

24

II

11

26

II

12

22

29

16

10

24

15

7
18

LOT B Lines

X
X

Selected for Elite line based on genome recovery data

Selected for QPM line based on genomerecovery data
selected for Elite & QPM both natures based on genome recovery data

X
X
X

X
X

X
X

X
X

X
X
X
X

X
X
X
X
X

Introduction of carotenoid superior alleles controlling the flux towards -carotene

TSS
E2 E3

E1

E4 E5

E6

E7

E8

E9 E10 E11 E12

E13

LCYE Gene Structure 5393bp (1778bp)

MGLSGATISAPLGCCVLRCGAVGGGKALKADAERWRRAGWSRRVGGPKVRCVATEKHDETA
AVGAAVGVDFADEEDYRKGGGGELLYVQMQSTKPMESQSKIASKLSPISDENTVLDLVIIGCGP
AGLSLASESAKKGLTVGLIGPDLPFTNNYGVWEDEFKDLGLESCIEHVWKDTIVYLDNNKPILIG
RSYGRVHRDLLHEELLKRCYEAGVTYLNSKVDKIIESPDGHRVVCCDKGREIICRLAIVASGAAS
GRLLEYEVGGPRVCVQTAYGVEVEVENNPYDPSLMVFMDYRDCFKEEFSHTEQENPTFLYAM
PMSPTRVFFEETCLASKDAMSFDLLKKRLMYRLNAMGIRILKVYEEEWSYIPVGGSLPNTDQK
NLAFGAAASMVHPATGYSVVRSLSEAPRYASVISDILGNRVPAEYMLGNSQNYSPSMLAWRTL
WPQERKRQRSFFLFGLALIIQLNNEGIQTFFEAFFRVPRWMWRGFLGSTLSSVDLILFSFYMFAI
APNQLRMNLVRHLLSDPTGSSMIKTYLTL
Zeamays Lycopene cyclase (lcyE) mRNA, lcyEW22 allele
Complete cds GenBank EU924262.1 (1778bp; 537aa)
354

60
181

ATG

207

126
152

88

156

57
197

98

TAG

HYD3 gene structure 4990bp (1246)

MAAAMTSFVAKNPLLAAAARRRAPPLAGRALPFSPLASTRAPRRTVTCFVPQDTAAPAAPVPALDEE
ARAAAARRVAEKEARKRSERRTYLVAAVMSSLGVTSMAVAAVYYRFSWQMEGGEVPVIETLGTFAL
SVGAAVGMEFWARWAHRALWHASLWHMHESHHRPREGPFELNDVFAIVNAAPAISLLAYGFFHRG
IVPGLCFGAGLGITLFGMAYMFVHDGLVHRRFPVGPIADVPYFRRVAASHKIHHMDKFGGVPYGLFL
GPKELEEVGGLDELVSSPVSEATDTEDAGEEKTRPVVCVVRTSVFMGQSVPNEF
Zeamays -carotene hydroxylase (HYD3) mRNA , B73 allele
Complete cds; GenBank: AY844958 1246bp (319aa)

AU

0.020

0.010

0.000
0.00

2.00

4.00

6.00

8.00

10.00

12.00

14.00

16.00

18.00

20.00

22.00
24.00
Minutes

26.00

28.00

30.00

32.00

34.00

36.00

38.00

40.00

42.00

44.00

5732

High -carotene
carotene accumulating donor maize line

AU

0.020

0.010

0.000
0.00

2.00

4.00

6.00

8.00

10.00

12.00

14.00

16.00

18.00

20.00

22.00
24.00
Minutes

26.00

28.00

30.00

32.00

34.00

36.00

38.00

40.00

42.00

44.00

2.00

4.00

6.00

8.00

10.00

12.00

14.00

16.00

18.00

20.00

22.00
24.00
Minutes

26.00

28.00

30.00

32.00

34.00

36.00

38.00

40.00

42.00

44.00

20.00

22.00
24.00
Minutes

26.00

28.00

30.00

32.00

34.00

36.00

38.00

40.00

42.00

44.00

0.040

AU

0.030
0.020
0.010
0.000
0.00

0.15
0.10
AU

M183 3003 3001

To Transform NSL Corn Crop Commercial lines with Unique Selling Proposition (USP)

0.05
0.00
0.00

2.00

4.00

6.00

8.00

10.00

Lutein Zeaxanthin

12.00

14.00

16.00

18.00

-carotene
carotene

NSL Corn Crop Commercial lines used in its NMH Hybrid breeding program

Details of LCY
LCY gene introgression for Marker-Assisted
Marker
Breeding
LCYE 5 indel (2)

A
F2

250bp
(A)

F1

R2

LCYE 5 indel (3)

D

O R

F2

R2
250bp + 380bp
(A )
LCYE 3 indel (8)

F2

Gene
LCY

HYD

Class
A
B
C
D
E

: 2= 250bp; 3=250 + 380bp

NQMPL's
Polymorphism
5' indel
3' indel
5' indel
3' indel
DEL4

5730
D
2
8
1
1
0

5732
D
2
8
2
1
12

5723
D
2
8
2
1
12

5721
D
2
0
2
1
12

B: 8= 8bp insertion (140bp); 0=132bp

5734
O
3
0
1
3
0

3001
R
2
0
1
2
0

3003
R
0
1
4
0

5896
R
2
0
1
1
0

C: 1=800bp; 2=600bp

5891
R
0
1
2
0

5881
R
0
1
1
0

M199
R
2
0
1
2
0

M183
R
2
8
1
3
0

M111
R
2
0
1
2
0

140bp
(B)
M184
R
0
1
2
0

D: 1= 543bp; 2 = 296bp; 3 = 296 + 1221bp; 4= 296 +875bp

R2

Favourable
Marker Type PCR-Assay
Alleles
2
8
2
1
12

STS-Indel
STS-Indel
STS-Indel
STS-Indel
STS-Indel

Agarose
Agarose
Agarose
Agarose
Agarose

E: 0= 117bp; 12=12bp insertion (129bp)

Gel %
2.5
3
2.5
2.5
3

Details of HYD3
HYD3 gene introgression for Marker-Assisted
Marker
Breeding
HYD3 5 indel(C)
(397bpinsertion)

H1

800bp(1)
(206bpinsertion)

H2

H1

H2

600bp(2)

HYD3 3 indel (D)

350bp

190bp

L1

R2
325bp

L1

3
L1

E
Non Specific Bands
D

R2

1250bp
R1

R2
HYD3 DEL4 (E)
12bpinsertion

D4F

D4R

D4F

D4R

Gene
LCY

HYD

Class
A
B
C
D
E

: 2= 250bp; 3=250 + 380bp

NQMPL's
Polymorphism
5' indel
3' indel
5' indel
3' indel
DEL4

5730
D
2
8
1
1
0

5732
D
2
8
2
1
12

5723
D
2
8
2
1
12

5721
D
2
0
2
1
12

B: 8= 8bp insertion (140bp); 0=132bp

5734
O
3
0
1
3
0

3001
R
2
0
1
2
0

3003
R
0
1
4
0

5896
R
2
0
1
1
0

C: 1=800bp; 2=600bp

5891
R
0
1
2
0

5881
R
0
1
1
0

M199
R
2
0
1
2
0

M183
R
2
8
1
3
0

M111
R
2
0
1
2
0

M184
R
0
1
2
0

D: 1= 543bp; 2 = 296bp; 3 = 296 + 1221bp; 4= 296 +875bp

Favourable
Marker Type PCR-Assay
Alleles
2
8
2
1
12

STS-Indel
STS-Indel
STS-Indel
STS-Indel
STS-Indel

Agarose
Agarose
Agarose
Agarose
Agarose

E: 0= 117bp; 12=12bp insertion (129bp)

Gel %
2.5
3
2.5
2.5
3

Is it possible to increase more -carotene?

Introgress -carotene
carotene accumulating lines into High Zeaxanthin maize lines
0.16

0.12
0.10
AU

3876

0.14

0.08
0.06
0.04
0.02
0.00
0.00

2.00

4.00

6.00

8.00

10.00

12.00

14.00

16.00

18.00

20.00

22.00
24.00
Minutes

26.00

28.00

30.00

32.00

34.00

36.00

38.00

40.00

42.00

44.00

2.00

4.00

6.00

8.00

10.00

12.00

14.00

16.00

18.00

20.00

22.00
24.00
Minutes

26.00

28.00

30.00

32.00

34.00

36.00

38.00

40.00

42.00

44.00

32.00

34.00

36.00

38.00

40.00

42.00

44.00

AU

0.08
0.06
0.04
0.02
0.00
0.00

0.020
AU

5732

3113

0.10

0.010

0.000
0.00

2.00

4.00

6.00

8.00

10.00

12.00

14.00

16.00

18.00

20.00

22.00
24.00
Minutes

26.00

28.00

30.00

Line Development - NSL Corn Crop Commercial lines used in its NMH Hybrid breeding program

Elite Line development with carotenoid superior

rior alleles for back crossing into QPM fixed lines
S.No

1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20

Rabi 2016
cross
nature
field code
R1516-172
HOMO
1107X5732
TO 174
HETERO
HOMO
R1516-134 1077X5730
HETERO
HOMO
R1516-186 1065X5723
HETERO
R1516-215
HOMO
1112X5716
TO 218
HETERO
HOMO
R1516-135 1079X5730
HETERO
R1516-139
HOMO
1082X5730
TO 141
HETERO
HOMO
R1516-181 1120X5732
HETERO
R1516-182
HOMO
1064X5723
TO 185
HETERO
R1516-203
HOMO
1106X5723
TO 205
HETERO
R1516-155
HOMO
1091X5730
TO 156
HETERO
R1516-136
HOMO
1080X5730
TO 137
HETERO
R1516-170
HOMO
1071X5732
TO 171
HETERO
R1516-187
HOMO
1066X5723
HETERO
TO 190
HOMO
R1516-211 1162X5723
HETERO
HOMO
R1516-138 1081X5730
HETERO
HOMO
R1516-131 1075x5730
HETERO
R1516-175
HOMO
1111X5732
TO 178
HETERO
R1516-192
HOMO
1094X5723
TO 196
HETERO
R1516-152
HOMO
1090X5730
TO 154
HETERO
R1516-132
HOMO
1076X5730
TO 133
HETERO

Total
47
47
44
44
41
41
46
46
40
40
40
40
49
49
38
38
45
45
43
43
42
42
48
48
40
40
50
50
42
42
24
24
49
49
49
49
47
47
50
50

%
Hyd5'TE
34.0
17.0
0.0
0.0
29.3
19.5
0.0
0.0
0.0
0.0
0.0
0.0
20.4
34.7
28.9
7.9
31.1
8.9
0.0
0.0
0.0
0.0
16.7
20.8
32.5
10.0
18.0
10.0
0.0
0.0
0.0
0.0
0.0
0.0
16.3
20.4
0.0
0.0
0.0
0.0

Hyd3'TE
0.0
0.0
22.7
13.6
14.6
29.3
0.0
0.0
2.5
40.0
37.5
20.0
29.4
28.6
23.7
10.5
0.0
0.0
7.0
32.6
23.8
9.5
4.2
29.2
25.0
12.5
22.2
40.0
14.3
4.8
20.8
12.5
0.0
0.0
16.3
20.4
6.4
17.0
14.0
6.0

Del4
34.0
17.0
0.0
0.0
26.8
22.0
0.0
0.0
0.0
0.0
0.0
0.0
12.2
42.9
31.6
10.5
31.1
8.9
0.0
0.0
0.0
0.0
25.0
4.2
25.0
20.0
16.0
12.0
0.0
0.0
0.0
0.0
22.4
12.2
16.3
20.4
0.0
0.0
0.0
0.0

Lcye3'TE
31.9
12.8
31.8
4.5
31.7
17.1
41.3
0.0
32.5
27.5
32.5
27.5
24.5
28.6
23.7
13.2
22.2
22.2
20.9
25.6
16.7
16.7
22.9
12.5
25.0
12.5
24.0
10.0
23.8
9.5
29.2
8.3
24.5
8.2
28.6
8.2
21.3
6.4
26.0
6.0

S.No

21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40

Rabi 2016
field
R1516149 TO
R1516142
R1516143 TO
R1516145 TO
R1516148
R1516157 TO
R1516165 TO
R1516163 TO
R1516200 TO
R1516197 TO
R1516199
R1516206 TO
R1516208 TO
R1516212 TO
R1516219 TO
R1516222 TO
R1516224 TO
R1516227 TO
R1516230 TO
R1516234 TO

cross
1089X5730
1084X5730
1086X5730
1087X619
1088X5730
1092X5730
1069X5732
1161X5730
1098X5723
1095X5723
1097X5723
1121X5723
1123X5723
1164X5723
1100X5721
1101X5721
1102X5721
1103X5721
1109X5721
1113X5721

nature

Total

HOMO
HETERO
HOMO
HETERO
HOMO
HETERO
HOMO
HETERO
HOMO
HETERO
HOMO
HETERO
HOMO
HETERO
HOMO
HETERO
HOMHO
HETERO
HOMO
HETERO
HOMO
HETERO
HOMO
HETERO
HOMO
HETERO
HOMO
HETERO
HOMO
HETERO
HOMO
HETERO
HOMO
HETERO
HOMO
HETERO
HOMO
HETERO
HOMO
HETERO

40
40
45
45
45
45
50
50
44
44
50
50
45
45
50
50
48
48
49
49
46
46
47
47
40
40
48
48
50
50
46
46
37
37
44
44
53
53
43
43

%
Hyd5'TE
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
21.7
2.2
0.0
0.0
32.5
35.0
35.4
0.0
26.0
0.0
32.6
0.0
13.5
0.0
0.0
0.0
32.1
1.9
30.2
2.3

Hyd3'TE
17.5
12.5
13.3
24.4
22.2
0.0
18.0
14.0
18.2
0.0
0.0
0.0
13.3
46.7
16.0
44.0
18.8
27.1
0.0
0.0
19.6
2.2
0.0
0.0
21.5
42.9
31.3
2.1
26.0
0.0
21.7
8.7
13.5
0.0
0.0
0.0
9.4
7.5
0.0
0.0

Del4
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
26.7
37.8
0.0
0.0
16.7
25.0
0.0
0.0
21.7
2.2
0.0
0.0
22.5
47.5
33.3
2.1
26.0
0.0
26.1
6.5
13.5
0.0
31.8
0.0
30.2
3.8
25.6
2.3

Lcye3'TE
20.0
5.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
26.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
18.4
0.0
0.0
0.0
27.7
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0

(Part -1)

Elite Line development with carotenoid superior

rior alleles for back crossing into QPM fixed lines
S.No
41
42
43
44
45

Rabi 2016
field code
R1516-242
TO 244
R1516-240
TO 241
R1516-251
TO 252
R1516-248
TO 250
R1516-267
TO 268

cross
5883x5732
5883x5730
1017x5730
5883X5716
5881X5730

46

R1516-266 5891X5730

47

R1516-299 5884X5730

48
49
50
51
52
53

R1516-344
TO 348
R1516-300
TO 301
R1516-295
TO 298
R1516-274
TO 275
R1516-253
TO 256
R1516-289
TO 291

10602X5723
M184X5730
M114X5730
5881X5723
1017X5723
M111X5723

54

R1516-343 10603X5723

55

R1516-310 M114X5732

56
57
58
59
60

R1516-285
TO 287
R1516-311
TO 313
R1516-314
TO 316
R1516-269
TO 273
R1516-351
TO 352

M111X5732
5894X5732
10589X5732
5881X5732
10590X5716

nature

Total

homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero

48
48
48
48
46
46
48
48
42
42
48
48
43
43
38
38
49
49
44
44
21
21
22
22
19
19
38
38
47
47
49
49
47
47
50
50
49
49
37
37

%
Hyd5'TE
37.5
8.3
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
34.2
7.9
0.0
0.0
0.0
0.0
9.5
9.5
45.5
9.1
31.6
5.3
21.1
13.2
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0

Hyd3'TE
29.2
12.5
22.9
10.4
21.7
4.3
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
34.2
7.9
0.0
0.0
0.0
0.0
0.0
0.0
31.8
13.6
31.6
5.3
13.2
18.4
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0

Del4
27.1
14.6
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
34.2
7.9
0.0
0.0
0.0
0.0
9.5
4.8
40.9
13.6
31.6
5.3
13.2
18.4
12.8
0.0
28.6
8.2
23.4
0.0
12.0
10.0
26.5
10.2
0.0
0.0

Lcye3'TE
29.2
14.6
29.2
12.5
21.7
6.5
29.2
0.0
19.0
0.0
18.8
0.0
27.9
0.0
26.3
15.8
26.5
0.0
25.0
0.0
14.3
9.5
27.3
31.8
31.6
15.8
28.9
5.3
0.0
0.0
22.4
6.1
0.0
0.0
22.0
6.0
30.6
10.2
27.0
0.0

S.No
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80

Rabi 2016
field
R1516276
R1516263 TO
R1516292 TO
R1516317
R1516353 TO
R1516337 TO
R1516329 TO
R1516325 TO
R1516318
R1516279 TO
R1516336
R1516303 TO
R1516302
R1516342
R1516321 TO
R1516319 TO
R1516357 TO
R1516355 TO
R1516349
R1516350

cross
5881X5716
5896X5716
M111X5716
10604X5732
10603X5716
10608X5732
10594X5732
10603X5732
10590X5732
5892X5730
10597X5732
10600X5730
5894X5730
10594X5723
10591X5732
10599X5732
10601X5716
10591X5716
6192X5723
6188 X 5723

nature

Total

homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetero
homo
hetro
homo
hetero
homo
hetero
homo
hetero

45
45
36
36
31
31
49
49
40
40
46
46
45
45
45
45
43
43
43
43
50
50
43
43
43
43
41
41
40
40
48
48
47
47
46
46
48
48
48
48

%
Hyd5'TE
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
17.8
0.0
15.6
24.4
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
15.0
5.0
18.8
10.4
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0

Hyd3'TE
0.0
0.0
0.0
0.0
0.0
0.0
12.2
0.0
0.0
0.0
23.9
10.9
22.2
0.0
17.8
13.3
14.0
11.6
11.6
2.3
8.0
0.0
20.9
16.3
16.3
0.0
12.2
0.0
15.0
5.0
14.6
0.0
0.0
0.0
0.0
0.0
16.7
4.2
16.7
0.0

Del4
0.0
0.0
0.0
0.0
0.0
0.0
16.3
0.0
0.0
0.0
23.9
10.9
20.0
2.2
26.7
17.8
20.9
11.6
0.0
0.0
10.0
2.0
0.0
0.0
0.0
0.0
17.1
0.0
17.5
2.5
18.8
10.4
0.0
0.0
0.0
0.0
22.9
4.2
0.0
0.0

Lcye3'TE
22.2
0.0
16.7
0.0
16.1
0.0
0.0
0.0
17.5
0.0
21.7
8.7
0.0
0.0
33.3
13.3
20.9
14.0
34.9
7.0
0.0
0.0
27.9
11.6
0.0
0.0
0.0
0.0
20.0
7.5
31.3
8.3
29.8
0.0
19.6
0.0
8.3
16.7
0.0
0.0

(Part -2)

Thank You!
Food security is National

Security