Documenti di Didattica
Documenti di Professioni
Documenti di Cultura
Experimental Procedure
Reagents
Literature Cited
Procedure
1. Dry Na2 EDTA2H2O for about one hour in the oven;
cool in a desiccator.
2. Prepare the pH 5.5 buffer by combining 1.35 g of
glacial acetic acid (C AUTION!) and 10.25 g of sodium acetate
(or 17.01 g of sodium acetate trihydrate) in a 250-mL volumetric flask and diluting to the mark. Adjust pH to 5.5 with
either 6 M NaOH or glacial acetic acid.
1463