Documenti di Didattica
Documenti di Professioni
Documenti di Cultura
Biology
IB1
Effect of the substrate concentration on enzyme catalysed
reaction
Introduction:
Enzymes are large globular protein molecules folded to form a three
dimensional globular structure, found in all living organisms. The biological
processes that occur within all living organisms are chemical reactions, and
most are regulated by enzymes. They are used to speed up specific reactions
in the cells. Without enzymes, many of these reactions would not take place
at an observable rate.
Hydrogen peroxide (H2O2) is a by-product of metabolism and is made in all
living cells. It is poisonous and must be removed as soon as it is produced in
the cell. Cells make the catalase to remove hydrogen peroxide.
Catalase is an enzyme found in living cells. It is found in food such potato,
carrot, liver, but also in yeast. Catalase is used to speed up the
decomposition of hydrogen peroxide into oxygen and water shown in the
equations given below.
Catalase
Hydrogen Peroxide -----------------------------Water + Oxygen
Catalase
2H2O2
2H2O + O2(g)
Enzymes bind with chemical reactants called substrates. There may be one
or more substrates for each type of enzyme, depending on the particular
chemical reaction. They are specific in their action. For an enzyme to
catalyze a reaction, a substrate has to be present and to bind with the active
site as a result of collisions due to random movement.
Hypothesis:
I believe that the increase in the concentration of the substrate
concentration will increase the rate of the reaction. If the concentration of
substrate increase, there will be more frequent collisions between the
substrate molecules and the enzyme active site, so the rate of reaction
increases. The rate of the reaction increases because there are more
substrate molecules added and it collides and bind with the available active
sites on catalase. This results in more no. of reactions so the amount of
oxygen released also happens quickly. But it will only increase to a certain
point, after which the rate of reaction will reach to its optimum level or
plateau and show no further increase by further increasing the concentration
of the substrate concentration. This is because the substrate molecules
exceed in the no. and there will be no active site available to collide and bind
to.
2
At a low substrate concentration there are many active sites that are not
occupied. This means that the reaction rate will be low.
Variables:
How to control them
Dependent
Controlled
Uncontrolled
Apparatus:
Risk Assessment
Wash hands with liquid hand wash after using hydrogen peroxide.
Method:
Prepare the yeast suspension 1 hour before the experiment by adding dry
yeast with lukewarm water. It will be used as an enzyme in this
experiment for all trials ensuring that the enzyme concentration is
constant in all trials. The amount of yeast suspension will be measured
using the 5 cm3 syringe.
Make a table to note the time it would take each concentration of the
hydrogen substrate to produce 35 cm3 of oxygen in the gas syringe.
Sources:
http://www.nuffieldfoundation.org/practical-biology/investigating-enzyme-controlledreaction-catalase-and-hydrogen-peroxide-concentrat
http://www.reviewmylife.co.uk/blog/2008/06/05/the-effect-of-substrateconcentration-on-the-activity-of-the-enzyme-catalase/#Extension
http://www.coolscience.org/CoolScience/KidScientists/h2o2.htm