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MICROBIOLOGY
The Latest Version A+ Study Guide
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After reading Ch. 6 in Microbiology: Principles and Explorations, fill in the following
matrix.
List the four phases of bacterial growth and briefly describe what happens in each
phase.
List and describe at least four methods used to measure bacterial growth.
List at least two physical factors that influence bacterial cell growth. Briefly describe
how bacterial growth responds to changes in those factors.
List at least two nutritional factors that influence bacterial cell growth. Briefly
describe how bacterial growth responds to changes in those factors.
List and describe at least two events that occur during the process of endospore
formation (sporulation).
Note: Visit Chapter 6 of Microbiology: Principles and Explorations in WileyPlus to view the
Endospore Formation Animation found under the Animated Concepts Section.
Shape Types:
Comma, or Club-shaped, rods = Vibrio
Rods = Coccobacillus, Bacillus
Spherical = Coccus
Spiral or Helical = Spirillium, Spirochete
Review Figure C on the following page. Use Figure C to identify the type of arrangement displayed by
the unknown bacteria. Read through the Sample Identification on the following page for an example of
how to identify bacterial groups and arrangements using Figures A, B, C, and the Gram stain results.
Figure C: Arrangement of Bacteria
Sample Identification
Rods and Clubs
After performing a Gram stain, you observe
Single
Chinese Letters
Cocci
Single
DiploTetrad
continue to item 3.
Sarcinae
Staphylo-
Strepto-
Now use Figure C to describe arrangement of bacteria. (The bacteria are not rod- or club-shaped,
so focus on the cocci arrangements.) Identify the arrangement that is most prominent.
Application
Use Figures A, B, C, and Gram stain results to identify group and arrangement of bacteria. Continue to
Part Two after completing the table.
(stained red)
(stained purple)
(stained purple)
(stained red)
(stained red)
(stained purple)
Group of Bacteria
Arrangement
Gram-negative cocci
Diplo-
Group of Bacteria
Arrangement
(stained purple)
Gram stain:
ONPG
ADH
LDC
ODC
CIT
H 2S
URE
TDA
IND
VP
GEL
GLU
MAN
INO
SOR
RHA
SAC
MEL
AMY
ARA
Gram-negative
Bacteria: Unknown
Based on Gram stain results and the knowledge that this bacterium caused food poisoning,
the laboratory is able to narrow down the possibilities to three bacterial strains. Figure 3
shows the API results:
Figure 3. API Results of Known Bacterial Strains in Text Format
Gram stain:
ONPG
ADH
LDC
ODC
CIT
H 2S
URE
TDA
IND
VP
GEL
GLU
Gram-negative
Bacteria: Salmonella
Gram stain:
MAN
INO
SOR
RHA
SAC
MEL
AMY
ARA
ONPG
ADH
LDC
ODC
CIT
H 2S
URE
TDA
IND
VP
GEL
GLU
MAN
INO
SOR
RHA
SAC
MEL
AMY
ARA
ONPG
ADH
LDC
ODC
CIT
H 2S
URE
TDA
IND
VP
GEL
GLU
MAN
INO
SOR
RHA
SAC
MEL
AMY
ARA
Gram-negative
Bacteria: Shigella
Gram stain:
Gram-negative
Bacteria: E. coli
Application
Compare the API biochemical test results in Figure 2 (unknown bacteria from produce) with
API test results in Figure 3 (known bacterial strains identified in the gray boxes on the left).
The bacterial strain in Figure 3 that matches Figure 2 will identify the bacterial strain causing
illness. Based upon your observations, which of the bacteria in Figure 3 is the most likely
cause of the food poisoning outbreak?
After reading Ch. 11 in Microbiology: Principles and Explorations, complete the following
matrices to compare and contrast different parasitic organisms. You may need to use the
index to help you locate some of the information needed.
PROTOZOA/MASTIGOPHORANS
Organism
Common Name
Trypanosoma
Location in Host
Nervous system
Mode of
Geographic
Transmission
Location
Tsetse fly
(Ch. 24)
Giardia
Ingestion of fecal
(Ch. 22)
Worldwide
motile trophozoites
Trichomonas
Vaginal
(Ch. 20)
inflammation
Worldwide
Select one of the organisms in this section. Describe the disease it causes, then compare similarities
or contrast differences in modes of transmission of the selected organism with any other organism in
any other section in this document. Focus on structures, conditions, or behaviors that make disease
transmission successful:
SPOROZOANS
Organism
Plasmodium (Ch.
11 & 23)
Common Name
Location in Host
Red blood cells
Mode of
Geographic
Transmission
Location
Tropical areas
Toxoplasma
Toxoplasmosis
Worldwide
gondii
(Ch. 23)
Select one of the organisms in this section. Describe the disease it causes, then compare similarities or
contrast differences in modes of transmission of the selected organism with any other organism in any
other section in this document. Focus on structures, conditions, or behaviors that make disease
transmission successful:
FUNGI
Organism
Common Name
Location in Host
Mode of
Geographic
Transmission
Location
Candida albicans
Can be sexually
Worldwide
(Ch. 19)
transmittted
Trichophyton
Poor hygiene or
(Ch. 19)
suppressed immune
Worldwide
system
Histoplasma
Inhalation
(Ch. 21)
Central and
eastern United
States and major
river valleys
worldwide
Select one of the organisms in this section. Describe the disease it causes, then compare similarities or
contrast differences in modes of transmission of the selected organism with any other organism in any
other section in this document. Focus on structures, conditions, or behaviors that make disease
transmission successful:
HELMINTHS/FLUKES
Common Name
Location in Host
Organism
Schistoma
japonicum
urinary bladder
Mode of
Geographic
Transmission
Location
Worldwide
(Ch. 23)
Describe the disease caused by Schistoma japonicum, then compare similarities or contrast differences
in modes of transmission of the organism in this section with any other organism in any other section in
this document. Focus on structures, conditions, or behaviors that make disease transmission
successful:
TAPEWORMS
Organism
Common Name
Taenia saginata
Tapeworm
(Ch. 22)
disease
Location in Host
Mode of
Geographic
Transmission
location
Uncooked beef
Describe the disease caused by Taenia saginata, then compare similarities or contrast differences in
modes of transmission of the organism in this section with any other organism in any other section in
this document. Focus on structures, conditions, or behaviors that make disease transmission
successful:
ADULT ROUNDWORMS
Organism
Common Name
Location in host
Wucheria
Mode of
Geographic
transmission
location
Mosquitoes
Tropical areas
ROUNDWORM LARVAE
Organism
Common Name
Symptoms in host
Borrelia
Mode of
Geographic
transmission
location
Ticks
Parts of the
burgdoreri (Ch.
United States,
23)
flu-like symptoms,
Australia, and
Europe
heart damage
Rickettsia
Fever, headache,
rash
Rickettsia
Lice
Worldwide
prowazekii (Ch.
23)
Select one of the organisms in this section. Describe the disease it causes, then compare similarities
or contrast differences in modes of transmission of the selected organism with any other organism in
any other section in this document. Focus on structures, conditions, or behaviors that make disease
transmission successful:
ANTHROPODS/ARACHNIDS
Organism
Common Name
Location in Host
Trichinella
Larvae in striated
muscle
Mode of
Geographic
Transmission
Location
Worldwide
Describe the disease caused by Trichinella spiralis, then compare similarities or contrast differences in
modes of transmission of the organism in this section with any other organism in any other section in
this document. Focus on structures, conditions, or behaviors that make disease transmission
successful:
Review Figure 10.1 of Microbiology: Principles and Explorations. Determine what the functions of
the following components are:
Component
Function
Capsid
Envelope
Nucleic acid
Spikes (glycoproteins)
Respond to Critical Thinking Question 1 in Ch. 10 of Microbiology: Principles and Explorations in
the space that follows.
Using two or three sentences, explain in your own words how viruses are classified by type of
nucleic acid, capsid shape, and presence of envelope.
Explanation
Nucleic acid
Capsid shape
Presence of envelope
Review Ch. 10 of Microbiology: Principles and Explorations. Briefly describe each of the stages of
viral reproduction.
Stage
Description
Absorption
Penetration
Synthesis
Maturation
Release
Respond to the following question from Ch. 10 of Microbiology: Principles and Explorations in the
space that follows. Looking at the five steps in viral replication, can you identify some promising
ways to prevent or control viral infections?
Respond to the following question from Ch. 10 of Microbiology: Principles and Explorations in the
space that follows. Would you classify viruses as living, nonliving, or semiliving? Explain your
answer.
Review Table 10.7 of Microbiology: Principles and Explorations. Define the following types of
viruses and viruslike agents.
Virus/Viruslike agent
Definition
Satellites
Viroids
Prions
Respond to the following questions in the space that follows. Can viruses cause cancer? Does it
matter if the virus is composed of DNA or RNA? Explain your answers.
completing
the
Inflammatory
Response
multimedia
activity
located
at
http://corptrain.phoenix.edu/hhs/SCI250/innate_defenses.html
complete the table below. List the four events of the inflammatory response covered in the activity in
the correct order of occurrence. As you re-write the events in your own words, add more detail and use
terms from Ch.16 of the text.
EVENTS IN AN INFLAMMATORY RESPONSE
Event
st
nd
rd
th
Type
of
Innate
Defense
Physical Barrier
at
restaurant
ingests
food
After reading Ch. 16-18 in Microbiology: Principles and Explorations, fill in the
following matrix.
Answer the following Checklist Exercise from Ch. 17 of Microbiology: Principles and
Explorations: Distinguish between active and passive immunity. Give examples of
each.
Answer the following Checklist Exercise from Ch. 17 of Microbiology: Principles and
Explorations: Distinguish between innate and acquired immunity. Give examples of
each.
Answer the following Checklist Exercise from Ch. 17 of Microbiology: Principles and
Explorations: Distinguish between cellular and humoral immunity.
In your own words, briefly explain what happens in a primary immune response. Be
sure to incorporate the following terms into your explanation: antigen, B-cell,
antibody, plasma, and memory cell.
In your own words, briefly explain what happens in a secondary immune response.
Incorporate the following terms in your explanation: memory cell and antibodies.
In your own words, briefly explain why a secondary immune response is much faster
than a primary immune response.
In your own words, briefly explain what the difference is between active and passive
immunization. Incorporate the following terms in your answer: vaccine, antigen,
attenuated, primary immune response, antibodies, serum, and immediate immunity.
Answer the following Checklist Question from Ch. 17 of Microbiology: Principles and
Explorations: What are some hazards of vaccines?
1.
2.
3.
4.
Which structures of the integumentary system are affectedUse one or more structures
included in Part Two.
Who is at risk
Create a tri-fold pamphlet, such as one you would find at a doctors office, about a
sexually transmitted disease of your choice using the template provided in Appendix G
located on the student website.
Address each of the elements in the grading form provided in the document titled
Appendix G Rubric located on the student website, and answer the following questions:
Is the disease transmitted exclusively through sexual contact or can it be spread
nonsexually?
What are its symptoms, and how can it can be diagnosed? (Be sure to compare a
healthy system to one affected by the infection or disease.)
What epidemiological and clinical impact does the disease or infection have?
How can the infection or disease be prevented?
Note. Feel free to modify the template by changing font sizes and colors and adding
graphics or pictures. If, however, you use graphics or pictures, please be sure to cite their
sources according to APA standards. Formatting help is available
at http://office.microsoft.com/en-us/default.aspx
1. How do body reflexes like coughing and sneezing help prevent or fight infection?
2. What role do cilia play in maintaining a healthy respiratory system?
3. Compare normal microflora of the upper respiratory tract with normal microflora of the
lower respiratory tract.
PART TWO: BASIC STRUCTURES
Visit Chapter 21 of Microbiology: Principles and Explorations in WileyPlus to view an in-depth
presentation of a healthy respiratory system by completing the following steps:
Select the Chapter 21 WileyPlus reading link located on your student Web page.
Locate the heading Chapter Review.
Select the Anatomy Overview: The Respiratory System link.
Complete this lab as you explore the Respiratory System multimedia piece.
Overview
Roll over each component of the Conducting and Respiratory portions of the Respiratory
System multimedia piece. Take note of how the preserved trachea and lung photograph on
the left compares to the illustrated diagram on the right.
Upper Respiratory System (Conducting Portion: Nasal Cavity)
Roll over and click either the nasal cavity or pharynx components of the Conducting portion
of the Respiratory System multimedia piece to navigate to the Nasal Cavity. Refer to Nasal
Cavity component of the multimedia piece and Ch. 21, Figure 21.8 in Microbiology: Principles
and Explorations to label the structures in the following diagram of a healthy upper respiratory
system.
Type answer
here.
Type
here.
answer
answer
Type
here.
answer
Type
here.
answer
Type
here.
answer
Which structures of the respiratory system are affectedUse one or more structures
included in Part Two.
Who is at risk
question
1. Catarrhal stage with fever, sneezing, vomiting, and mild cough; paroxysmal stage with
ropy mucus and violent cough; convalescent stage with mild cough
Whooping cough
Classic pneumonia
Legionnaires' disease
Tuberculosis
Influenza
Histoplasmosis
Cryptococcosis
Aspergillosis
Whooping cough
Classic pneumonia
Legionnaires' disease
Tuberculosis
Influenza
Histoplasmosis
Cryptococcosis
Aspergillosis
3. Inflammation of the lungs, fever, chills, headache, diarrhea, vomiting, and fluid in lungs
Whooping cough
Classic pneumonia
Legionnaires' disease
Tuberculosis
Influenza
Histoplasmosis
Cryptococcosis
Aspergillosis
4. Tubercles in lungs and sometimes in other tissues; organisms can persist in walled-off
lesions and be reactivated
Whooping cough
Classic pneumonia
Legionnaires' disease
Tuberculosis
Influenza
Cryptococcosis
Aspergillosis
5. Viruses subject to antigenic variation, with new strains causing epidemics; inflammation of
oropharyngeal membranes, fever, malaise, muscle pain, cough, nasal discharge, and
gastroenteritis
Whooping cough
Classic pneumonia
Legionnaires' disease
Tuberculosis
Influenza
Histoplasmosis
Cryptococcosis
Aspergillosis
Whooping cough
Classic pneumonia
Legionnaires' disease
Tuberculosis
Influenza
Histoplasmosis
Cryptococcosis
Aspergillosis
7. Fever, kidney abnormalities; in severe cases shock, bleeding, and pulmonary edema
Whooping cough
Classic pneumonia
Legionnaires' disease
Tuberculosis
Influenza
Histoplasmosis
Cryptococcosis
Aspergillosis
Whooping cough
Classic pneumonia
Legionnaires' disease
Tuberculosis
Influenza
Histoplasmosis
Cryptococcosis
Aspergillosis
Whooping cough
Classic pneumonia
Legionnaires' disease
Tuberculosis
Influenza
Histoplasmosis
Cryptococcosis
Apsergillosis
10. Usually a mild pulmonary disease; pneumonia and dissemination to meninges can occur
Whooping cough
Classic pneumonia
Legionnaires' disease
Tuberculosis
Influenza
Histoplasmosis
Cryptococcosis
Aspergillosis
familiar with a healthy system and to identify diseases related to this system.
PART ONE: BASIC FUNCTIONS
Provide brief answers to the following questions to help you get acquainted with the basic
functions of a healthy cardiovascular system. Refer to Ch. 23 in Microbiology: Principles and
Explorations.
5. What are the main functions of the cardiovascular system?
6. Which types of bacteria are considered normal cardiovascular system microflora?
7. Which structure of the cardiovascular system is particularly susceptible to bacterial
infection?
PART TWO: BASIC STRUCTURES
Refer to Ch. 23 in Microbiology: Principles and Explorations to help you identify the selected
cardiovascular system structures in the following diagram.
Type
here.
answer
Type
here.
answer
Type
here.
answer
Rheumatic fever
Bacterial endocarditis
Describe the causal agent of the selected disease, mode of transmission, disease
characteristics, and the component(s) of the cardiovascular system that are damaged by the
disease.
Gram-positive bacteria.
Gram-negative bacteria.
fungi.
viruses.
Staphylococcus aureus
3. What organism can cause cutaneous infections and can be transmitted by endospores?
Staphylococcus aureus
Pseudomonas aeruginosa
Yersinia pestis
Bacillus anthracis
Streptococcus pyogenes
4. A pathogen of both humans and cattle that causes undulant fever is a member of the
genus ________
Yersinia.
Borellia.
Brucella.
Pediculus.
Streptococcus.
5. Both deer and black-legged ticks are involved in the transmission of the agent of Lyme
disease, which is caused by ________
Ixodes scapularis.
Borrelia burgdorferi.
Rickettsia akari.
Bartonella bacilliformis.
Yersinia pestis.
6. A tumor of the jaw and viscera (Burkitt's lymphoma) occurs approximately six years after
a primary infection with what virus?
Ebola virus
Dengue virus
Epstein-Barr virus
Epstein-Barr virus.
Ebola virus.
dengue virus.
Epstein-Barr virus.
Coxsackie viruses.
Streptococcus pyogenes.
Staphylococcus aureus.
Erythrovirus B19.
9. The presence of a single gene for sickle-cell anemia protects individuals against malaria
by ________
10. Mild lymph node inflammation in adults; can cross placenta and cause serious damage to
nervous system of fetus; also causes damage in small children and immunosuppressed
patients
Dengue fever
Yellow fever
Infectious mononucleosis
Malaria
Toxoplasmosis
Complete the open-book quiz about the nervous system and associated diseases. The
quiz will be provided by your instructor.
Nervous System Lab Week Seven
INTRODUCTION
The nervous system has two components: the central nervous system, which consists of the
brain and spinal chord; and the peripheral nervous system, which is composed of nerves. As
you learn about the various diseases that affect the nervous system, it is important for you to
understand the structures that can be affected by disease. Complete this lab to become
familiar with a healthy system and to identify diseases related to the brain, spinal chord, and
nerves.
PART ONE: BASIC FUNCTIONS
Provide brief answers to the following questions to help you get acquainted with the basic
functions of a healthy nervous system. Refer to Ch. 24 in Microbiology: Principles and
Explorations.
4. How do the meninges and cerebrospinal fluid (CSF) function together in a healthy
nervous system; what are their roles?
5. What is the blood-brain barrier?
6. What normal microflora reside in the nervous system?
PART TWO: BASIC STRUCTURES
Visit Chapter 24 of Microbiology: Principles and Explorations in WileyPlus to view an in-depth
presentation of a healthy nervous system by completing the following steps:
Select the Chapter 24 WileyPlus reading link located on your student Web page.
Locate the heading Chapter Review.
Select the Anatomy Overview: The Nervous System link.
Complete this lab as you explore the Nervous System multimedia piece.
Nervous System: The Brain
Roll over each component of the Nervous System multimedia piece. Click on the brain. Refer
to the Brain component of the multimedia piece to label the structures in the following diagram
of a healthy brain.
BONUS: Meninges
answer
SCI250 Week 7 Chapter 24 Nervous System Quiz each question worth .25 point (10
Streptococcus pneumoniae
Escherichia coli
Staphylococcus
Haemophilus influenzae
3. Which of the following bacteria that may cause meningitis is Gram positive and therefore
does not cause endotoxin shock in infected individuals?
Escherichia coli
Neisseria meningitidis
Listeria monocytogenes
Haemophilus influenzae
4. An immunofluorescent antibody test (IFAT) has replaced the older test for the presence of
inclusions in neurons (Negri bodies) for the detection of infections caused by ________
Neisseria meningitides.
Enteroviruses.
Mumps virus.
Rabies virus.
Haemophilus influenzae.
Togaviruses
Enteroviruses
Mumps virus
Rabies virus
Hepatitis viruses
Naegleria fowleri
Mycobacterium leprae
Streptococcus pneumoniae
Neisseria meningitidis
Haemophilus influenzae
the trypanosomes change their surface glycoproteins evading the host immune
response.
A and B
8. A major difference between infections with prions and other agents is that infections with
prions:
9. Invades nerves and brain; headache, fever, nausea, partial paralysis, coma, and death
ensue unless patient has immunity
Bacterial meningitis
Listeriosis
Rabies
Encephalitis
Hansen's disease
Tetanus
Botulism
Pliomyelitis
Chagas' disease
10. Shrinkage and lysis of neurons of the central nervous system; headache, fever, and
sometimes brain necrosis and convulsions
Bacterial meningitis
Listeriosis
Rabies
Encephalitis
Hansen's disease
Tetanus
Botulism
Poliomyelitis
Chagas' disease
20/20 Correct
Section: Multiple Choice
1. Which of the following is NOT true of microorganisms?
They are used in the production of many types of food and beverages.
lag phase
stationary phase
doubling phase
log phase
Gram stain
Schaeffer-Fulton
acid-fast stain
flagellar stain
4. In a properly executed Gram stain, Gram positive organisms appear ______ while Gram
negative organisms appear ______
pink, clear.
pink, purple.
purple, pink.
purple, blue.
host.
commensal.
parasite.
symbiont.
Schistosoma.
Tania.
Wuchereria.
Trichinella.
viral
bacterial
protozoan
9. All of the following are true of the relationship between microorganisms and diseases
EXCEPT:
10. Which of the following represents the correct order of steps in a typical viruses replication
cycle?
11. What special group of chemical substances are used to treat diseases by microbes?
Antibiotics
Synthetic drugs
Semi-synthetic drugs
Antimicrobial agents
Chemotherapeutic agents
12. Which of the following is NOT considered a mode of action by antimicrobial agents?
Action as an antimetabolite
13. All of the following characteristics are components of nonspecific host defenses EXCEPT:
Fever
Mucus
Antibodies
Interferon
Inflammation
14. What name is given to a substance that is recognized by the body as foreign and
normally produces an immune response?
Antibody
Antigen
Agglutinin
Antitoxin
Apoptosis
15. There are a number of different species that can live on the skin as resident microflora.
Which of the following is NOT considered resident microflora of the skin?
Staphylococcus
Corynebacterium
E. coli
Demodex
16. Defense mechanisms in the urogenital system are numerous and include ________
sphincter muscles.
low pH.
17. Food poisoning can be caused by a variety of microbes. Which of the following pairs is
incorrect?
Only A and C
19. In closed environments, such as military bases, dormitories, and day-care centers, 90%
of the population can be carriers of this organism, yet only 1 per 1000 develops the
meningitis. What is the etiology?
Neisseria meningitidis
Haemophilus influenzae
Haemophilus influenzae
Streptococcus pneumoniae
Listeria monocytogenes
improper storage.
importation.
Only A and B