Documenti di Didattica
Documenti di Professioni
Documenti di Cultura
3/2010 (150-180)
ISSN 0946-2104
Abstracts
Metaliomics: a fundamentai
approach in mechanisticaiiybased metai toxicoiogy
research.
E. Sabbioni
Ageing Research Center (CeSI),
"G d'Annunzio" University
Foundation, Chietl, Italy
Scientific research on biometals
has generally been carried out separately in many fields such as biochemistry, nutritional science., medicine,
pharmacy, toxicology, agriculture and
environmental science, with "metals"
as common factor. However, a better
promotion of the biometals science as
interdisciplinary field to integrate the
metal-related scientific areas requires
a more general approach, in this context, metaliomics has been recently
151
proposed as a new frontier in the investigation of trace elements in biology [1]. Metal ions are utilized by
fundamental cellular processes. In
this context, transcription, translation
and incorporation of the adequate
metal to function are regulated by the
organism, being synthesis and metabolic function of genes (DNA, RNA)
and proteins not possible without the
aid of metal ions and metalloenzymes. Thus, the biochemistry of a cell
needs to be characterized not only in
its peculiar genetic (genome) and protein (proteome) context but also by
the distribution of all chemical forms
of the metals/metalloids (metallome)
at different levels of complexity
(whole body, organ., cell and individual biomoIecule).Thus, the purpose
of metaliomics (study of metals and
metal species and tbeir interactions,
transformations and functions in biological systems) is to extend and
broaden the bioinorganic chemistry
as a "systematic scientific field" and
an interdisciplinary science complementary and in symbiosis with other
"omics" such as genomics, proteomics and metabolomics. From the experimental point of view, metaliomics
involves the determination of a metal
in whole cells, its distribution over
different cell compartments, its association with particular pools (macromolecules, small molecules, free
ions) and the binding to a specific
class of biomolecules. These versatile
measurements require the availability
of advanced and sophisticated analytical tools to reach an adequate specificity of the analytical response with
regard to the identification of a particular metal species, and enough sensitivity to allow the ultratrace detection
and quantitation of covalently bound
metals in microsamples of proteins
and metal-protein complexes. As
such, metaliomics, referred to cellular
biochemistry, can be considered a
subset of speciation analysis (distribution of an element among defined
chemical species in a system). The
objectives of this work are to present
examples of an experimental in vitro
mechanistically-based dynamic metaliomics research. In particular, the
aims are (i) to highlight the role of nuclear and radio analytical techniques
(neutron activation analysis and use
of radiotracers with high specific radioactivity) in combination with advanced spectochemical (GF-AAS,
References
[i]
7th International symposium on trace elements in human: new perspectives, October 13th - 15th 2009, Athens, Greece
152
153
Abstracts
Acknowledgment
This study was supported by
ECNIS Network of Excellence (Contract No. 513943/UE).
7th Internationai symposium on trace elements in human: new perspectives, October 13th - 15th 2009, Athens, Greece
8vn
chmlcBl groups of amIno add
wed as dietary supplment:
Ala, It, Lflu. Val I Arg, His, Lye I
TTir, Sir I Glu, Atp) Met, Cye I Try. Phe I Pro
Practicable Radioisotopes
N.V. Phlllps-Duphar, compiled by J.JJ^men
1
Ha
II
IV
VI
VH
VUI
Tl V Cr Mn Fe,Co
K
Cu
Rb
Ag
Sr
Sn
Mo
154
Objective: The use of complementary and alternative methods is common in oncology, but nothing is known
about the role of CAM in the last days
of a patient's life. Material and Methodx Between 1-2007 and 10-2008 the
palliative care team treated 85 patients (50 male, 35 female, median
age 62 years) on out-door basis. All
families were asked to complete a
structured questionnaire regarding
the use of CAM during the last period
of a patient's life. Patients suffered
from cancer in 83/85 patients (98%),
two patients had neurologic disorders. Results: Only 20 families (31 %)
have not used any CAM. Spiritual
support (praying, hospice services)
was for asked by 60/85 families
(71 %). 25/85 families (29%) reported
drug use. Favorite methods were vitamins (n = 11, 13%), trace elements
(n - 9, 11%), and mistletoe (n - 8,
9%). 30 patients (35%) were treated
by physiotherapy (28 oncology patients, 2 neurology patients). LogoReferences
[ 1 ] Tallberg Th. Westermarck T, Atroshi F. A pedy and ergotherapy were used in 10
patients (12%). 12 families (14%) repossibility to prevent recurrent melaported aroma-therapy in patient's care.
noma.renal. breast and prostate cancer J.
Main information source was the GP
Trends in Biomedicine. 2008; 3: 44-54.
(house physician) (45/85, 53%)), followed by the palliative care team
(30/85, 35%) and pharmacies (27/85,
32%). Internet, journals or self-service information were used by only
25/85 families (29%). The effectivity
of the categorized methods was esti-
Abstracts
mated between I and 5 (very good worthy) by the patients or their families. The following ranking was registered: spiritual support 2.4; physical
therapy 2.7, Iogopedy/ergotherapy
3.1, drugs 3.3. 65/85 families (76%)
were satisfied with the information
received from the health care practitioners. Conclusion: Complementary
methods are often practiced during
the last days of a patient. Families and
friends are looking for valid information and need help by the professionals. The different methods have to be
evaluated regarding effectivity as
well as acceptance by the patients.
155
creased the concentration of selenium
in the serum of our patients was.
These fmdings are supported by new
data of NMR-tumor volumetry and
trace elements concentrations in 21
patients. The relation between serum-selenium and tumor volume is
stronger than between classical tumor
markers (SCC; CEA, CYFRA 21-1)
and the tumor volume. A last investigation has suggested that a destroyed
homeostasis of trace elements is a
sign of pre-terminal ill patient. When
the destroyed homeostasis is observed, the patient has a window of
4 - 6 weeks of remaining life time.
Conclusions: Trace element status offers a lot of information about the
course of the disease and the individual. But environmental factors have
more impact on this trace element status than on other classical tumor
markers.
7th International symposium on trace elements in human: new perspectives, October 13th - 15th 2009, Athens, Greece
156
157
Abstracts
Diabetes Type 2:
A new perspective
D. Oberleas
Department of Food and Nutrition,
Texas Tech. University, Lubbock,
TX, USA
There is mounting evidence that
chromium'^ is required as a cofactor
for the normal function of insulin. An
adequate supply of insulin is synthesized and circulating in most Type 2
diabetics. For insulin to function
properly it must attach to an insulin
receptor in the cell wall and form a
stable attachment for a short period of
time. However, in the absence of
disulfide cross-linking, protein:protein interactions are not stable. A
small peptide with molecular weight
of 1500 has been isolated and functions like a chaperone by complexing
chromium'* to insulin receptors and
subsequently to insulin to form a stable union, fhc stable union creates a
quantum of light and production of
HiO:. Other symptoms associated
with Type 2 diabetes are quite variable among patients and are a result of
the variety of sub-clinical deficiencies described previously. Zinc is involved in every normal cell division
in the body. Zinc is also important in
the maintenance of normal thymus
morphology and function. Thus, secondary infections have an increased
prevalence and extended infectivity
that may result in physiological crises. Copper deficiency symptoms are
accentuated and may result in increased hemorrhagic strokes, aortic
aneurisms and cardiac aneurisms.
Many diabetics suffer from leg
cramps and have cholesterol quality
problems that may be effectively
treated with magnesium supplementation. Nickel, boron and other ultra-trace elements may also have minor contributions towards normal
physiology associated with Diabetes
II. It is not clear why these symptomologies may be drastically accentuated by the elevation of circulating
glucose whose major effect is
increased osmotic pressure.
7th International symposium on trace elements in human: new perspectives, October 13th - 15th 2009, Athens, Greece
Acknowledgment
The author gratefully acknowledges Dr. Christina D. Chambers,
Prof Carl. L. Keen (University of
California) and Elena S. Vyatchanina
(Russian Society of Trace Elements in
Medicine) for collaboration in FASD
studies.
158
Trace elements in
hemochromatosis
B.J. Bolann and R.J. Ulvik
Institute of Medicine, University of
Bergen, and Laboratory of Clinical
Biochemistry, Haukeland University
Hospital, Bergen, Norway
Hemochromatosis is a hereditary
disorder characterized by increased
iron uptake fiom the gut, which over
the years may result in iron accumulation in the body and iron overload.
The condition can be treated successfully by bloodletting. Bleeding of 450
ml of blood is performed every 1 - 2
weeks until the patient's iron status is
normalized. Then bloodletting 2 - 5
times a year is continued lifelong to
prevent new accumulation of iron.
Some uptake, transport and storage
mechanisms used by iron are shared
with trace elements. The increased
iron absorption, iron overload in the
tissues, and the removal of trace elements with the bloodletting, may all
afTect trace element metabolism.
Abstracts
159
the present study, including 61 alcoholic patients and 12 age and sexmatched controls, all of them sanitary
workers who had undergone a cranial
scan because of minor symptoms. Patients showed a more intense degree
of atrophy, differences being observed in Huckmann (t ^ 2.95), bicauda (t - 3.93) and celia index (t 3.75) and cortical atrophy (t ^ 5.45,
p < 0.001 in all cases). Statistically
significant differences between patients with cerebellar atrophy and
those without cerebellar atrophy were
observed regarding copper (854 |ig/I
181 ^ig/l vs. 1088 [ig/\ 269 ng/l,
p = 0.014), and zinc (971 ^g/1 696
Hg/1 vs. 1817 \ig/\ 1298 \ig/lp =
0.03). On the contrary, serum ferritin
was higher among patients with cerebellar atrophy (529 370 ng/ml vs.
308 209 ng/ml p - 0.044). Serum
ferritin also showed a significant correlation with bicaudate index (rho ^
0.3, p = 0.029) and Huckmann's index (rho = 0.32, p = 0.02), and serum
transferrin, an inverse one with Huckmann s index (rho ^ -0.30, p = 0.031 ).
However, selenium showed a direct
(opposite of what was expected) significant correlation with Huckmann
index (rho = 0.35, p - 0.046). No relation was observed between trace elements and age, amount of ethanol
consumed, years of consumption,
malondialdehyde (only 12 cases), or
proinfiammatory cytokines (TNF-a,
IL-6). Thus, besides ferritin, higher
among those with more intense atrophy, trace elements are only poorly related to brain atrophy, but patients
with cerebellar atrophy do have lower
serum copper and zinc levels.
Dietary bio-moduiation
schedule for treatment of
prostate cancer patients
T. Tallberg', T. Westermarck' and
F. Atroshi"
'The Helsinki Institute for
Bio-Immunotherapy Ltd., and
^Pharmacology and Toxicology,
ELTDK, University of Helsinki,
Helsinki, Finland
Prostate cancer (CaP) is a hormone-dependent disease based on
Huggins and Hobbs orchiectomy
studies in 1941 [1]. As CaP regularly
recurred, it led to bilateral adrenaiectomies [2], but in spite of this all pa-
tients died in a short time since adrenal glands actually had a central regulatory position in this endocrine disease [3]. CaP still lacks a definitive
comprehensible etiology. It is in many
cases a potentially incurable agelinked disease. Several clinical observations designate that a regulatory
function is performed by the adrenal
glands linked to a feed-back effect activated by its zona-reticularis (ZR)
cells. This positive clinical effect is
promulgated by specific dietary metabolic factors in all stages of CaP [4].
These nutritional factors have been
found to cure CaP even in cases suffering from multiple bone mtastases
[5] - to avoid hormone refractory
states (HRPC), and stop the progress
of the disease in patients on Watchful
Waiting [6]. We have used dietary
bio-modulation schedule for treatment of prostate cancer patients (Table 1). This complex dietary formula
has been developed empirically over
two decades according to the aenocarcinoma code for CaP [4, 6]. The
amino acids, trace-element ions and
CNS lipids tested were fed according
to the code while constantly monitoring the effect on the patients' hormone balance and clinical features.
Dietary bio-modulation schedule for
treatment of prostate cancer patients.
1) Oral administration of each ( 2 - 5
g/day) of respective L-amino acids;
Arg, Asp, Glu, Gly, Lys, and Ser,
eventually also His, all in connection
with meals. 2) Essential trace-element
salts prescribed orally as biologically
active ions, at dose levels of some
milligrams ( 1 - 3 mg/day); Chromium
(CrCl2.6H:O) 6 mg (- 1.17 mg Cr),
Tinn (SnCl4.5H2O) 4 mg (= 1.35 mg
Sn), Strontium (SrCb) 1 - 7mg {- 1
mg Sr), Vanadine (Na: VO.,. 4 H2O), 6
mg (- 2.5 mg V), Wolfram (Na. WO4.
2 H:0), 4 mg (- 2.3mg W). 3) Small
physiologic amounts of vitamins; A,
B, C, D, E, K, folie acid (2 mg/d) and
lycopene. To improve lymphopoiesis
and the immune-defence of patients a
diet containing prion-free neurogenic
lipids (Neurofood Ltd. Finland). All
these dietary ingredients can be mixed
together in yogurt or ice-cream, or as a
daily nutritional ration utilizing prepacked powders. Dose-levels are adjusted based on the clinical response
as measured during the therapy, and
correlated to the patients' body
weight. A good prognosis caused by
adrenal ZR feedback activation can
7th International symposium on trace elements in human; new perspectives. October 13th - 15th 2009, Athens, Greece
References
[11
Huggins C. Hodges CV. Studies on prostate cancer: I. Effect of castration, estrogeti. and androgen injection on serum
phosphatases in metastatic carcinoma of
the prostate. Cancer Research. !')4I; I:
[2]
Huggins C. Scon WW. Bilateral adrenalectomy iti prostate cancer. Ann Surg.
\945\ 122: 1031-1041.
Tallherg T. Cancer treatment, based on
active nutritional bio-modulation, hormottal therapy and specific autologous
immunotherapy. J Aust Coll Nutr and
EnvMed. N96; 15:5-2^.
Tallbei-g T. Studies on cancer of tlie Prostate Gland, a search for Aetiological and
Prognostic Factors. J Aust Coll Nutr and
EnvMed. 2003; 22.- 11-16.
Tallherg T Regulation of cancer by therapeutic vaccination and dietary bio-modulation involving organ specific mitochondria. Int J Biotechnology. 2007; 9:
391-410.
Tallherg T. Dahek M. Prostate cancer,
aetiological, therapeutic, prognostic and
prophylactic factors. Anticancer Research. 2008; 28: 3507-3508.
[3]
[4]
[5]
[6]
Bio-Immunotherapy can
prevent recurrent prostate renal cancer, and melanoma
T. Tallberg, T. Westermarck and
F. Atroshi
The Institute for Bio-Immunotherapy,
Helsinki, Finland
Tumors may spontaneously regress which implies that mammals
possess a natural intrinsic regulatory
capability to control the de-differentiation of specialized organ cells, "The
metabolic triumph of the host" (Dr.
W.M. Cole 1974). Thus this novel
paradigm is that cancer represents a
complex chronic metabolic deficiency disease which can be compensated by biological dietary means.
The aim was consequently to analyze
if the disturbed interior milieu can be
corrected /compensated and lead to a
biological cure without side-effects in
prostate cancer patients. Randomized
clinical studies with hundreds of cancer patients, over 35 years, showed
highly significant (p - 0.001) im-
160
161
Abstracts
lipids in affecting three specific segmentai areas of the spinal cord is possibly also linked to different forms of
clinical atherosclerosis. Mitochondria! regulation is related to lipidomics, genomics and proteomies, and
it may lead to the future use of
schooled organ-specific mitochondria as genuine cancer remedies.
7th Intemational symposium on trace elements in human: new perspectives, October 13th - 15th 2009. Athens, Greece
162
Abstracts
163
Our results indicate that tobacco
smoking during pregnancy affected
pro-hepcidin levels in serum of mothers and their newboms. Low concentrations of some iron markers in umbilical cord blood suggest that mother's
smoking could lead to subclinical iron
deficiency in fetus.
7th International symposium on trace elements in human: new perspectives, October 13th - 15th 2009, Athens, Greece
164
mass fraction of Li (milligram per kilogram of dry bone) in rib-bone of females, males, and females and males
taken together were as follows:
0.0360 0.0024, 0.0376 0.0020,
and 0.0369 0.0015, respectively. No
clear correlation was found between
the Li content and age and gender. A
comparison of the Li mass fraction in
the rib-bone witb the Li whole blood
mass fraction for reference of men
shows that the bone accumulates at a
significantly higher (almost in 50
times) level than blood does. TTie results obtained may serve as indicative
normal values for the lithium content
in human rib-bone.
165
Abstracts
Selenoenzymes iodothyronine
deiodinases
A complex molecular machinery to specificaliy incorporate
selenocysteine into proteins
important for health and
disease
L. Wurth, A. Takeuchi,
M. Rederstorff, A. Leseare,
C. Amang and A. Kro!
The major biolgica! form of selenium is the 21st amino acid selenocysteine. It sets apart from the other
' Faculty of Veterinary Medicine,
amino acids because of its peculiar
Trakia University, Student's Cambiosynthesis pathway and incorporapus, Stara Zagora, 'Institute of
tion into selenoproteins in response to
Experimental Pathology and
a reprogrammed UGA codon. SelenoParasitoiogy, BAS, Sofia, Bulgaria
proteins constitute a family of oxidaand 'Medical School, University of
tion-reduction enzymes involved in a
Athens, Greece
variety of well-established important
The effect of sodium selenite on
biological functions, such as reductheantioxidantstatus in broiler chick- tion of reactive oxygen species, thyens infected with Eimeria acervulina roid hormone matia^tion, sperm matwas studied. Antioxidant status was uration and muscle development. A
measured via blood plasma malonyl complex machinery, comprising at
dialdehyde (MDA), serum glutathione least eight gene products, participate
peroxidase (GPx), erythrocyte catalase in this process. The essential compo(CAT) and Superoxide dismutase nents are the SECIS element, an RNA
(SOD) activities and plasma levels of stem-loop structure in the 3' untransselenium (Se), vitamins A, C and E lated region of selenoprotein mRNAs,
with respect to parasitolgica! and and the SECIS-binding protein SBP2.
production traits. The study was per- In addition to identifying the SBP2
formed on 100, 20-day old broiler amino acids and SECIS bases imporchickens. Four groups of chickens tant for SBP2-SECIS RNA complex
were formed: the 1st - control, the formation, we discovered that the as2nd - control and received Na^SeO^ sembly of selenoprotein mRNAs with
(0.3 mg/kg diet for 10 days), the 3rd - SBP2 and other factors important for
infected with 3 >< 105 sporulated E. recoding the UGA codon requires an
acerx'uUna oocysts and the 4th - in- adaptor protein and the Hsp90
fected with E. acefvidina oocysts and chaperone complex. Patients carrying
received the same dose N
mutations in the selenoprotein N gene
S. Pavelka'^
'Institute of Biochemistry, Faculty of
Science, Masaryk University, Brno,
and -Department of Radiometry,
Institute of Physiology, Acad. Sei.
CR, Prague, Czech Republic
We describe in general properties
of selenoenzymes iodothyronine
deiodinases (IDs) of Types 1, 2 and 3
(Dl, D2 and D3, respectively) and
their fianctions in the metabolism of
iodothyronines (thyroid hormones).
In particular, we describe several applications of our newly developed radiometric enzyme assays for determination of Dl, D2 and D3 activities in
specific samples of biological material. These assays were used to follow
the alterations in IDs activities caused
by short-time incubation of cultured
astroglia! ce!ls in a chemically defined medium with different concentrations of several effectors, such as
retinoic acid, adrenergic and purincrgic agonists, substances modulating
intracellular Ca^"^ concentration, etc.
Further, it was used to determine
changes in IDs activities in various rat
tissues as a response to the effects of
some exogenous factors, such as
subchronic treatment of the animals
with ami depressant fiuoxetine. With
the aid of our new enzyme assays, we
also measured the activity of D3 in
several depots of murine white adipose tissue to fo!low development of
dietary obesity in mice fed a high-fat
diet, compared to standard !ow-fat
diet.
Acknowledgements: This work
was supported by the Academy of
Sciences ofthe CR (Research project
No. AV0Z50110509), by the Ministry
of Education of the CR (Research
7th intemationai symposium on trace elements in human: new perspectives. October 13th - 15th 2009, Athens, Greece
166
Abstracts
167
Affects setenium indeed
concentrations of serum
autoantibodies TPOAb and
TGAb?
J. Kvicala', P. Hrd', V. Zamrazil',
J. Nemecek', M. Hill' and V. Jiranek'Institute of Endocrinology, and
'DataPro, Praha, Czech Republic
Many factors take part in the development of autoimmune thyroiditis
(AIT) - predominantly the genetic
predisposition, but also environmental etiologic causes. One of them
might be selenium. Selenium (Se) is
an essential component of enzymes
with an extensive regulatory and protective effect in an organism. Immunological effects of Se are documented and are distinct even above
concentrations necessary for maximal
activity of selenoenzymes. Effect of
supplementation by 100 ^g of yeastbound Se on concentrations of thyroid autoantibodies (Ab) TPOAb and
TgAb in the starting group of 349 seniors living in the Asylum Houses of
South Bohemia was monitored in the
course of three years. Se was analyzed
by Instrumental Neutron Activation
Analysis (INAA), Abs by ELISA,
both at the beginning of the trial and
after each year. Se increased from
59.9- 146.0 (Ig Se/I serum in supplemented group during the first year,
with subsequent maintaining of this
level during the next two years. Statistical evaluation of results in groups
regardless of increased Ab by ANOVA
manifested significant decrease of
TPOAb and TgAb in the course of the
first year. Decrease of TGAb continued while concentrations of TPOAb
increased during the next two years in
both supplemented and non-supplemented group. Changes were time-related. Evaluation of seniors with increased Ab revealed a similar time-dependent course but TPOAb was marginally affected by supplement. Recent results of possibility to affect serum concentration of TPOAb by Se
proved effect only for high TPOAb
concentrations. From this point of
view, it seems necessary to conduct
long-term trials with the patients with
different levels of Abs.
The work was partly supported by
grant IGA M2 CR No. NR/9160-3.
7th International symposium on trace elements in human: new perspectives, October 13th - 15th 2009, Athens, Greece
168
Interaction of antidepressant
drug fiuoxetine with the
metabolism of triiodothyronine
S, Pavelka'-^
'Institute of Biochemistry, Faculty of
Science, Masaryk University, Bmo,
and ^Department of Radiometry,
Institute of Physiology, Acad. Sei.
CR, Prague, Czech Republic
In the present study, we followed
the effects of subchronic administration (for 20 days) of antidepressant
drug fluoxetine, alone or in combination with 3,5,3'-triiodothyronine (T3),
on the metabolism of iodothyronines
(thyroid hormones) in the rat. In rats
treated with fiuoxetine together with
T3, we previously encountered a profound decrease in the preceding, experimentally elevated T3 plasma levels. Here, we intended to unravel this
observation. We used radiometric enzyme assays for iodothyronine sulfotransferases (ST) and UDP-giucuronyltransferase (GT), as well as our
newly developed radiometric assays
for iodothyronine deiodinases of
types 1,2 and 3 (D1, D2 and D3). The
measurements of ST and GT activities
in different fractions of peripheral tissues did not demonstrate any significant effects of the administration of
fiuoxetine, alone or together with T3,
on the induction of these enzymes.
However, treatment of rats with
fiuoxetine alone caused a moderate
increase in D2 and, in turn, a slight decrease in D3 activities in cerebellum
and some other regions of the CNS.
No significant changes in Dl activity
were detected. On the other hand, the
administration of T3 alone caused, in
accordance with our expectation, a
substantial decrease in pituitary D2
activity and a simultaneous increase
in Dl and D3 activities practically in
all tissues studied. In contrast with the
effects of T3 by itself, treatment of the
rats with the combination of fiuoxetine
plus T3 did not cause any significant
elevation of Dl and D3 activities.
Acknowledgements: This work
was supported by the Academy of
Sciences of the CR (Research project
No. AV0Z50110509), by the Ministry
of Education of the CR (Research
project No. MSM0021622413) and
by the Czech Grant Agencies GA CR
(Grant No. 304/08/0256) and GA AS
CR (Grant No. KJB401630701 ).
169
Abstracts
Iron supplementation of
fuii-term healthy breast fed
infants
J.K. Friel, M. Moffatt and A. Miller
Departments of Pediatrics and
Human Nutritional Sciences,
University of Manitoba, Winnipeg,
Manitoba., Canada
Full-term healthy breast-fed infants have previously been shown to
benefit fiom iron supplements early
in infancy. To test this hypothesis in
another population, 82 infants were
randomized to receive 7.5 mg of supplemental iron as ferrous sulphate (1)
or placebo (P) once a day from 1 - 6
months of age. All infants were breastfed at one month of age and were encouraged to continue exclusive breastfeeding to 6 months before introducing solid foods. Blood samples were
collected by venipuncture at 4 and 12
months of age. Anthropomtrie and
demographic data was collected at 1,
4, 6 and 12 months of age when a
Bayley Developmental assessment
was conducted. Although infants
weighed more at birth (P: 3.63 0.4
vs. 1: 3.42 0.5 kg p < 0.05) this difference did not persist after one
month of age. At 4 months ferritin (1:
110 91 vs. P: 75 48) was higher
and transferrin receptors (I: 20 4.4
vs. P: 22 6) were lower in the ironsupplemented group. At 4 months
GPx was higher (I: 10.7 3 vs. P:
9.3 2) which did not persist to 12
months. Bayley MDI (P: 99 9 vs. 1:
97 10) and PDI (P: 91 14 vs. 1:
92 14) did not differ. These results
suggest that full-term healthy breast
fed infants from an advantaged population will increase iron stores when
given iron supplements with no adverse effects on growth but no advantage for developmental outcome at
one year. Supported by CIHR and
MICH.
7th International symposium on trace elements in human: new perspectives, October 13th - 15th 2009, Athens, Greece
immunomodulation, regulation of
thyroid hormones metabolism and effects. The aims of the study was 1 ) to
compare the effect of supplementation by various Se species (yeastbound Se and selenite) on the Se status (serum Se, urine Se, daily urine Se
excretion., urine Se/creatinine ratio)
of seniors from 3 houses for seniors in
South Bohemia and 2) to compare effect of biologically bound Se on seniors and adults. Serum Se was analyzed by neutron activation analysis
(NAA) and urine Se by fiuorimetry,
both analyses with the use of biological standards. Analyses before the intervention trials proved low Se status
and intake both in seniors and adults.
Curves obtained by statistical evaluation of analyses proved different effects of organically and inorganically
bound Se. Higher increase of serum
Se in the course of intervention by
yeast-bound Se agrees with the theory
of partial nonspecific binding of
selenomethionine (semet) to any protein and the increase of serum Se concentration. Low urine Se concentration and mainly low urinary Se excretion in the group supplemented by selenite does not fit well with the theory
of increased excretion of inorganic
forms of Se by urine. Differences in
the curves of all measured and calculated Se indices in the trial of yeastbound Se intervention of seniors and
adults are indicative of differences in
regulatory mechanisms for Se metabolism in adults and seniors.
170
171
Abstracts
Conditions of the natural environment components according to ecological - geochemical monitoring and population
health data (Tomsk region,
Russia)
L.P. Rikhvanov', N.V. Baranovskaya',
A.M Betyaeva', L.V. Zhomyak',
A.V. Talovskaya', O.A. Denisovaand E.G Yazikov'
'Tomsk Polytechnic University, and
^Siberian State Medical University,
Tomsk, Russia
Scientific research in the Tomsk
region has been conducted by the staff
of the Geoecology and Geochemistry
Department, Tomsk Polytechnic University. The objects of the following
research in ecological geochemical
zoning of Tomsk region are soil, solid
precipitate of snow samples (dustaerosol formations of snow surfaces),
salt formations from crockery; (scale),
as well as children hair and human
thyroid gland. In addition, there is
sufficient information of such natural
environment sources as peat, lake
bottom sediments, tree age-rings which
are referred to as sfratified formations
indicating the chemical component
intake dynamics and further retrospective evaluation of natural environment transformations in time. Tbe
scientific research shows an intensive
flux of specific radionuclides and
other elements in the natural environment of the southern Tomsk region
during the second half of the 20th century (for example, U and Pu). Many
natural and anthropogenic characteristic factors influence microelements
accumulation level in the research
material. Data anaiysis shows specific
technogenic impact in oil and gas recovery regions (Sb, Br), coal and nuclear energy enterprises (La, U, Th).
Special attention was paid to the following fact - significant U and La accumulation level in children hair and
significant uranium accumulations in
thyroid gland pathology of the population. Ranging administrative areas on a
parameter of the general complex environmental contamination, and hygienic
ranging of territory of area on degrees
of a pressure of a sanitary-and-hygienic
situation and interrelation of these parameters with the population's health is
resulted.
Element composition of
human organs and tissue
N.V. Baranovskaya, L.P. Rikhvanov,
T.N. Ignatova, I.A. Matveenko, GP.
Sandimirova and N.N. Pakhomova
Tomsk Polytechnic University,
Tomsk, Russia
In the present state of biogeochemical investigations in a man, the
problem of quantitative determination of maximum possible concentration for chemical elements and their
ratios in human organs, tissues, and
organism both in normal and in pathologic state, has become utterly urgent.
Of no less importance is the revealing
of regional, national, sex-age differences in their accumulation. To
solve this problem we have attempted
to study chemical composition of a
man's organs and tissue living in
Eastern Siberia. Application of the
two high-precision methods of analysis: instrumental neuron-activation
and induction-connected plasma and
permits for determination of accumulation levels and characteristics of
distribution over the human organs
and tissue - inhabitant of Tomsk region
- 56 chemical elements. Distribution of
these elements in human organism is
extremely irregular and their variations
in concentration range up to several orders. Element accumulation in human
organs and tissues follows the general
geochemical laws of Klark and OddoGarkins for distribution of chemical
elements in the Universe. The accumulation level of elements in human
organism is determined by not only
biochemical and biophysical properties of their inctions in living tissue
but also sexual and presumably age
characteristics, pathological changes
as well as the factors of natural environment of a man. Information on accurate quantitative element composition of a man, in terms of the regional
and some other characteristics, could
contribute to more efficient correction of human health and prophylaxis
of different diseases.
7th International symposium on trace elements in human: new perspectives, October 13th - 15th 2009, Athens, Greece
172
173
Abstracts
7th International symposium on trace elements in human: new perspectives, October 13th - 15th 2009, Athens, Greece
174
Abstracts
[2]
175
[3]
7th International symposium on trace elements in human; new perspectives, October 13th - 15th 2009, Athens, Greece
Concentrations of metals in
welder's blood
1. Martinsone
Riga Stradins University, Riga,
Latvia
In Latvia, approximately 2.4% of
the workforce is involved in metal
processing industry. According to
laboratory measurements of metal
processing industry workplace air by
Riga Stradin's university Institute of
Occupational Safety and Environmental Health Laboratory of Hygiene
and Occupational Diseases, working
environment was estimated to be of
poor quality. The aim of this study
was to investigate metals concentrations in welder's blood, because it is a
widely used biomarker in different
countries. Overall 104 welders were
sampled for metals (manganese, chromium, nickel, and zinc, copper) concentrations in blood. The concentrations of metals in blood are very varied;
welders had higher metal levels of manganese and chromium in blood than the
recommended reference interval.
176
177
Abstracts
References
[1]
[2]
[3]
[4]
LongRS. Process for ihe recovery ofuranium from phosphatic ore, US Patent
2.882,123, 1959.
Rothbaum HP. McGaveston DA. Wall T.
Jahnston AE. Maltiiigly CEG Uranium
accutnulation in soils from long-continued applications of superphosphate. Eur
J Soil Sei, 1979; .fl,- 147-153,
Misra NL. Dhara S. Sing Mudher KD.
Uranium determination in seawater by
total reflection X-ray fluorescence spectrometry. Spectrochim Acta PartB. 2006;
61: 1166-1169.
Zarkadas Ch, Karydas AQ ParadelUs T.
Determination of uranium in human
urine by total refleclion X-ray fluorescence, Spectrochim, Acta PartB. 2001;
Jo. 2505-2511.
7th International symposium on trace elements in human: new perspectives, October 13th - 15th 2009, Athens, Greece
Assessment of 59 trace
element contents in rib bone
of healthy humans by
inductively coupled plasma
mass spectrometry
S. Zaichick', V. Zaichick^
V. Karandashev^ and I. Moskvina^
H. Bertelsmann, D. Behne,
M. Hammadeh and
A. Kyriakopoulos
Helmholtz-Zentrum Berlin fr
Materialien und Energie, Berlin,
Germany
Selenium in spermatozoa is linked
to male fertility. Two forms of glutathione peroxidase 4 are present in
mammalian sperm, the sperm nuclei
glutathione peroxidase (snGPx) and
the phospholipid hydroperoxide glutathione peroxidase (PHGPx). Different analytical methods including instrumental neutron activation analysis (INAA) and atomic absorption
spectrometry (AAS) were used to determine selenium in sperm. The investigation on the chemical form of
selenium in sperm is usually carried
out by immune detection on blot
membranes with an antibody suitable
for the simultaneous detection of
snGPx and PHGPx. However, this
method is in the best case a semiquantitative approach as these selenoproteins may show a different immune reactivity. For the quantification of all selenoproteins in sperm in
one experiment laser ablation coupled
to mass spectrometry or long-term irradiation with INAA, methods which
can be applied directly on gels with
separated selenoproteins, can be
useftil in future studies.
Characterization of human
selenoproteins (Sep 15 and
other) by HPLC-ICP/MS and
Specific ELISA
J. Bartel, T. Bartz, A. Sote, J. Bartel
and A. Kyriakopoulos
Helmholtz Zentrum Berlin fur
Materialien und Energie, Dept.
"Molecular Trace Element Research
in the Life Science", Berlin,
Germany
In previous decades a number of
studies established selenium as an element of fundamental importance to
human health. The trace element is a
component in selenium binding proteins and present in at least 25 selenocysteine containing proteins, summa-
178
Abstracts
179
mass spectrometry. Mean values (M
SEM) for the mass fraction of Li (milligram per kilogram) in scalp hair of
females, males, and females and
males taken together were as follows:
0.0221 0.0035, 0.0399 0.0056.
and 0.0317 0.0036, respectively. A
tendency for an increased Li mass
fraction with age was observed in the
scalp hair of females only. It was
shown that lower Li content was typical of female scalp hair as compared
to those in male hair. No clear correlation was found between the Li content
in scalp hair and rib-bone. The results
obtained may serve as indicative
normal values for the lithium content
in human scalp hair.
7th International symposium on trace elements in human: new perspectives, October 13th - 15th 2009, Athens, Greece
180
cant effect on the iodine determination result (a ^ 0.1). A good correspondence (for 10 additional determinations) between the certified (3.38
0.02 ppm with variation coefficient
(v.c.) of 0.59 % for standard reference
material (srm) and the measured iodine concentrations (3.52 0.29 ppm;
v.c. = 10 %) was achieved. The applied conditions of digestion, reinforced by the action of microwaves,
brought about a decidedly shorter
(less than 20 minutes) sample preparation time.
Acknowledgements
This work was supported by the
Academy of Sciences of the CR (Research project No. AV0Z50110509),
by the Ministry of Education of the
CR
(Research
project
No.
MSM0021622413) and by the Czech
Grant Agencies GA CR (Grant No.
304/08/0256) and GA AS CR (Grant
No. KJB401630701).
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