ALL INDIA SENIOR SECONDARY SCHOOL

CERTIFICATE
EXAMINATION (YEAR-2015-16)

Project report on

INVESTIGATION OF NICOTIN FROM

TOBACCO
SUBMITTED BY:
TO:

SUBMITTED

Saksham Gupta
Singh

Mr. Hardeep

Class XII F
Dept.
Roll no.-1644687

Chemistry

ACKNOWLEDGEMENT
I would like to express my sincerest gratitude
and own a grave sense of thankfulness to
MR.HARDEEP SINGH BSP,Pilani for his
incommensurate, in documentation and
guidance which inspired me to undertake this
project.
I am also thankful to MR.V.K.SHARMA, MR.
SURENDRA MEHTA, MR.GYAN PRAKASH AND
MR.PREM without whose help the successful
completion of the project would not have been
possible.
Saksham
Gupta
XII F

CERTIFICATE

This is to certify that this project

entitled INVESTIGATION OF
NICOTIN FROM TOBACCO has been
conceived by Saksham Gupta has
been completed under my supervision
and guidance.

Mr. HARDEEP
SINGH
P.G.T, Chemistry
BIRLA SCHOOL
PILANI

Contents
1. Introduction
2. Aim

3. Apparatus & Chemicals

4. Procedure
5. Apparatus setup diagram
6. Observation table
7. Result and inference
8. Bibliography

Introduction
Tobacco, the widely known drug is mainly present in
cigar, cigarette, beedi, suuff, etc. It belongs to the
family grown species are N rustica and N tobacco. One
of the harmful components of tobacco is Nicotine.
Nicotine is colorless volatile liquid. It boils at a
temperature of 246-247C and soluble in most solvents
including water. Pure nicotine is highly poisonous.It is
hygroscopic and distills over unchanged into steam. Aqueous
nicotine is strongly alkaline to litmus. It is easily soluble in

ethanol, light petroleum, ether and benzene. On exposure to air

it turns yellow and then
Brown. Nicotine is levorotatory. Nicotine is converted into
nitrogenous acid on oxidation with potassium permanganate or
chromic acid. Nicotine acid is a plant growth regulator and
vitamin. The structure of Nicotine is:-

AIM
The Aim of investigation is two-fold:1. Two extract nicotine from tobacco.
2. To determine the percentage of nicotine
in various tobacco products and make a
comparison.

PROCEDURE
About 10 grams of tobacco was grounded properly and
taken in a conical flask. Then 10 ml of conc. NaOH was
Added to it. The contents were warmed on a low flame
and stirred vigorously. This process was continued till a
strong spell of tobacco has mixed with NaOH properly,
the contents were cooled to room temperature. The
contents then were transferred to room temperature.
Then they were transferred to a separating funnel,
which had already been washed, and about 15 ml of
ether was added. Later, the contents were stirred
gently to ensure complete mixing. Some time was
allowed to elapse till the ether layer separated from
rest of the contents. Then the bottom layer of NaOH
was removed and poured back into the conical flask.
The ether was taken in a beaker and placed in a boiling
water bath to allow the ether to evaporate. The
extracted nicotine was weighed and its percentage was
calculated accordingly.

Conformation of nicotine was done by adding conc. HCL

to extracted nicotine. The quality of salt formed gave
an idea of strength of nicotine.

APPARATUS AND
CHEMICALS
Apparatus:
1.
2.
3.
4.
5.
6.

Conical flask
Separation funnel
Water bath
Beaker
Funnel
Test tube

Chemicals:
1.
2.
3.
4.

NaOH(conc.)
Ether
HCL(conc.)
Tobacco sample

OBSERVATION
TABLE
Table-1
S.NO

Name of
Brand

1
2

Ganesh
Nevla

Color of PPT.

THE % OF NICOTINE IN VARIOUS

SAMPLES ARE GIVEN BELOW

TABLE-2
S.NO NAME
OF
BRAND

Gane
sh

Weight
Weight of
of
Tobacco
Nicotine
sample

%Of
Nicotine=
Weight of
Nicotine
Extracted
weight. Of
Tobacco

2
3

Nevl
a
Hans

RESULT
Nicotine is successfully
extracted from various
tobacco samples and
percentage of nicotine is
calculated as follows:
Ganesh:Nevla:Hans:-

BIBLIOGRAPHY

Investigation projects
in chemistry by
A.Chakrabotry Tata Mc
Graw Hill Pulication.
Practical chemistry by
R.C. Khanna,
J.P.Publications