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diploid
B)
phosphodiester
C)
probe
D)
pyrimidine
E)
introns
F)
cloning
G)
transformed
H)
exons
I)
ampicillin
J)
autoradiography
K)
chain-terminator
L)
purine
3. Experiments by Avery and colleagues proved that DNA was the substance that _____ a nonpathogenic pneumococcus strain to a virulent strain.
Ans: G
Section 5-2. DNA is the Carrier of Genetic Information
Difficulty: Easy
4. Experiments by Avery and colleagues proved that DNA was the substance that _____ a nonpathogenic pneumococcus strain to a virulent strain.
Ans: G
Section 5-5. Molecular Cloning
Difficulty: Easy
7. Eukaryotic genes often contain regions that are transcribed and later excised from the RNA,
called ______.
Ans: E
Section 5-4. Gene Expression and Replication: An Overview
Difficulty: Easy
Multiple Choice
8. What group is attached to the ring in thymine and is not found on uracil?
A) ribose
B) CH3
C) NH3
D) OCH3
E) none of the above
Ans: B
Section 5-1. Nucleotides and Nucleic Acids
Difficulty: Easy
Ans: A
Section 5-1. Nucleotides and Nucleic Acids
Difficulty: Easy
10. The most common tautomeric form of the purine and pyrimidine bases in nucleic acids is
the:
A) enol form
B) imino form
C) keto form
D) ester form
E) none of the above
Ans: C
Section 5-3. Double Helical DNA
Difficulty: Easy
Ans: D
Section 5-5. Molecular Cloning
Difficulty: Difficult
Ans: E
Section 5-5. Molecular Cloning
Difficulty: Difficult
Ans: A
Section 5-5. Molecular Cloning
Difficulty: Moderate
Answer: B
Section 5-1. Nucleotides and Nucleic Acid
Difficulty: Moderate
16. Free nucleotides are almost always associated with ______ in cells.
A) proteins
B) cholesterol
C) Cl- counterions
D) fatty acids
E) Mg2+ counterions
Answer: E
Section 5-1. Nucleotides and Nucleic Acid
Difficulty: Easy
17. By convention, the sequence of nucleotide residues in a nucleic acid is written ___________
starting with the ____ end.
A) left to right; 3
B) right to left; 3
C) left to right; 5
D) right to left; 3
E) top to bottom; 3
Answer: C
Section 5-1. Nucleotides and Nucleic Acids
Difficulty: Easy
19. In a large part, Francis and Cricks proposal of a double-helical structure for DNA was based
on evidence from:
A) NMR (nuclear magnetic resonance) spectroscopy
B) IR (infrared) spectroscopy
C) atomic force microscopy
D) electron microscopy
E) x-ray crystallography
Answer: E
Section 5-3. Double Helical DNA
Difficulty: Easy
20. RNA occurs primarily as single strands, often giving rise to _______ structures.
A) diploid
B) stem-loop
C) parallel
D) tautomeric
E) haploid
Answer: B
Section 5-4. Gene Expression and Replication: An Overview
Difficulty: Easy
Answer: A
Section 5-4. Gene Expression and Replication: An Overview
Difficulty: Easy
22. Polynucleotides are cleaved at recognition sites within the strands by:
A) bacteriophages
B) DNA ligase
C) type III restriction endonucleases
D) reverse transcriptase
E) type II restriction endonucleases
Answer: E
Section 5-5. Molecular Cloning
Difficulty: Moderate
23. The production of multiple identical organisms from a single ancestor is called ________.
A) DNA ligation
B) phenotyping
C) cloning
D) transcription
E) sequencing
Answer: C
Section 5-5. Molecular Cloning
Difficulty: Easy
24. Small circular DNA molecules used to carry foreign DNA are called ________.
A) mRNAs
B) bacteriophage
C) clones
D) plasmids
E) nucleotides
Answer: D
Section 5-5. Molecular Cloning
Difficulty: Easy
25. In molecular cloning, transformed organisms must be identified. One common method for
accomplishing this involves the addition of a gene for ________.
A) transcription
B) X-gal
C) exonuclease activity
D) colony migration
E) antibiotic resistance
Answer: E
Section 5-5. Molecular Cloning
Difficulty: Moderate
Answer: B
Section 5-5. Molecular Cloning
Difficulty: Moderate
27. In situ hybridization often uses radioactively labeled probes. When probing for a gene
containing the sequence AGCTAACGGG (standard notation), the probe should have a
sequence containing
A) just TT; in order to recognize all related genes
B) TGCCC, in order to bind selectively to the 3 end of the gene of interest
C) CCCGTTAGCT, in order to very selectively bind the target in an antiparallel fashion
D) a gene conferring antibiotic resistance
E) DNA ligase
Answer: C
Section 5-5. Molecular Cloning
Difficulty: Moderate
Answer: D
Section 5-5. Molecular Cloning
Difficulty: Easy
Answer: E
Section 5-5. Molecular Cloning
Difficulty: Easy
30. Nucleoside triphosphates are useful for energy transfer in metabolic processes because the
triphosphate bonds are relatively ______.
A) stable
B) high energy
C) bio-compatible
D) large
E) low energy
Answer: B
Section 5.4. Gene Expression and Replication: An Overview
Difficulty: Easy
31. A new strand of DNA, produced using a given strand of DNA as a template, is :
A) an exact duplicate of the template.
B) a negative copy of the template.
C) a palindromic copy of the template.
D) an exact copy of the template but with the 3 and 5 ends reversed.
E) none of the above.
Answer: B
Section 5.2. DNA is the Carrier of Genetic Information
Difficulty: Moderate
Answer: E
Answer: B
Section 5.4. Gene Expression and Replication: An Overview
Difficulty: Easy
Answer: E
Section 5.5. Molecular Cloning
Difficulty: Moderate