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www.elsevier.com/locate/chemosphere
a,*
Department of Environmental Hydrology and Microbiology, Zuckerberg Institute for Water Research, Jacob Blaustein Institutes for Desert Research,
Ben-Gurion University of the Negev, Sede Boqer Campus, 84990 Midreshet Ben-Gurion, Israel
b
Department of Plant Science, Gilat Research Center, Mobile Post Negev 85280, Israel
Received 19 February 2006; received in revised form 4 June 2006; accepted 6 June 2006
Available online 17 July 2006
Abstract
The use of greywater for irrigation is becoming increasingly common. However, raw greywater is often contaminated and can cause
environmental harm and pose health risks. Nevertheless, it is often used without any signicant pretreatment, a practice mistakenly considered safe. The aim of this study was to develop an economically sound, low-tech and easily maintainable treatment system that would
allow safe and sustainable use of greywater for landscape irrigation in small communities and households. The system is based on a combination of vertical ow constructed wetland with water recycling and trickling lter, and is termed recycled vertical ow constructed
wetland (RVFCW). The RVFCWs properties, removal eciency, hydraulic parameters and feasibility were studied, as well as the environmental eects of the treated greywater, as reected by soil and plant parameters over time. The RVFCW was ecient at removing
virtually all of the suspended solids and biological oxygen demand, and about 80% of the chemical oxygen demand after 8 h. Fecal coliforms dropped by three to four orders of magnitude from their initial concentration after 8 h, but this was not always enough to meet
current regulations for unlimited irrigation. The treated greywater had no signicant negative impact on plants or soil during the study
period. The feasibility analysis indicated a return over investment after approximately three years. We concluded that the RVFCW is a
sustainable and promising treatment system for greywater use that can be run and maintained by unskilled operators.
2006 Elsevier Ltd. All rights reserved.
Keywords: Vertical ow constructed wetland; Wastewater reuse; Greywater treatment; Environmental pollution
1. Introduction
The quantity of freshwater available worldwide is
declining, raising the pressing need for its more ecient
use. One method of conserving water is by recycling greywater (GW) for irrigation. GW is domestic wastewater that
includes only wash water (i.e., bath, dish, and laundry
water), whereas blackwater consists of toilet water. Due
to the substantial dierence in their qualities, separating
GW and blackwater would provide for more eective
wastewater treatment, allowing a large volume of water
*
0045-6535/$ - see front matter 2006 Elsevier Ltd. All rights reserved.
doi:10.1016/j.chemosphere.2006.06.006
to be eciently recycled (Lindstrom, 2000). This is particularly important in arid zones, were water is scarce and
recycling GW for private and public landscape irrigation
could reduce potable water use by up to 50% (DHWA,
2002). The use of GW for private garden irrigation is
becoming increasingly common. In most countries, regulations or specic guidelines for GW reuse are not available,
and it is therefore often used without any signicant pretreatment, a practice mistakenly considered safe. In countries such as the USA and Australia, where regulations
for the use of GW have been established, they concentrate
on issues associated with public health but do not consider
potential harmful environmental impacts (Dixon et al.,
1999; DHWA, 2002; ADEQ, 2003). The separation of the
917
ous working period. This procedure ensured the development of bio-lm in the wetland and stabilization of the
system performance in terms of removal eciency and ow.
2.2.1. Short-term study
In the beginning of the study, the pore volume of the lter section and the treated water reservoir were emptied
and 300 l of fresh GW was introduced into the VFCW
(at the root zone). A subsample of the raw GW was collected for analysis (time zero). The GW was continuously
recycled between the reservoir and the VFCW at a known
rate of 390 l h1, determined by a water meter that was
attached to the system. Samples of the treated GW were
taken immediately after it initially passed the bed and then
after 2, 4, 8, 12, 24, and 48 h. Samples were analyzed for:
total suspended solids (TSS), total phosphorus (TP), total
nitrogen (TN), anionic surfactants measured as methylene
blue-active substances (MBAS), dissolved oxygen (DO),
electrical conductivity (EC), pH, 5-day biological oxygen
demand (BOD), chemical oxygen demand (COD), and
total boron (TB). Water-quality analysis followed standard
procedures (APHA, 1998); fecal coliforms (FC) and total
coliforms were measured by the plate count technique
(Merck, 2000).
2.2.2. Batch greenhouse study
The RVFCW was used to evaluate the environmental
eects of treated GW on plants and soils in comparison
918
with untreated GW and freshwater. GW was prepared articially by mixing 20 g of laundry detergent (Ariel Ultra,
Procter and Gambel, USA), 0.86 g of boric acid, and
400 ml of raw kitchen euents into 150 l of tap water to
resemble the GW quality at a nearby farm (Gross et al.,
2005). Every other day at 08:00, 150 l of treated GW was
removed from the reservoir and replaced with the articial
GW that was introduced into the root lter zone. Thirty
lettuce plants (Lactuca sativa), which are sensitive to water
quality, were used as the model plants for each treatment.
Plants were grown for 89 d (15 Feb 0215 May 02) in 5 l
pots lled with autoclaved quartz sand, and were drip-irrigated (300 ml plant1 d1) twice a day (at 07:00 and
17:00) by computerized irrigation system (Netam, Kibutz
Hazerim, Israel). The investigation was concluded once the
lettuce started to bud. Water samples of untreated GW,
treated GW and freshwater were collected three times a
month, 810 h after GW replacement, and analyzed for:
TSS, TP, TN, total ammonia nitrogen (TAN), nitrite
(NO2N), nitrate (NO3N), EC, pH, anionic surfactants
as MBAS, BOD, COD, TB, total coliforms, and FC. Samples were also analyzed for minerals and metals (Ca, K,
Na, Mg, B, Fe, Al, Cu, Mn, Zn) by inductively coupled
plasma (ICPAES) and atomic adsorption (AA) once a
month. Analysis followed standard procedures (APHA,
1998; Merck, 2000). The water-quality dierences among
the treatments were statistically analyzed by ANOVA
(p < 0.05).
Undisturbed 5 cm soil cores were taken monthly from
seven pots of each treatment and analyzed for: pH, organic
carbon (OC), total Kjeldahl nitrogen (TKN), minerals and
metals (Ca, K, Na, Mg, B Fe, Al, Cu, Mn, Zn). Analysis
followed standard procedures (Soil and Plants Analysis
Council, 1999). For the FC count, ve undisturbed cores
(6 g wet weight from depths of 5 cm) from each treatment
were put into sterile tubes. Pyrophosphate buer (6 ml) was
added and samples were shaken for 1 h. The supernatant
was used for FC count on Chromocult TBX (Tryptone Bile
X-glucuronide) agar plates by the pour plate method
(Merck, 2000). Soil sub samples were analyzed for moisture
content and the FC count was calculated on dry weight
base.
The plants from these pots were tested for wet and dry
weight, leaf weight and surface area, number of leaves,
and minerals and metals as already described.
2.2.3. Long-term case study
Based on the results, sizing and operational procedures
were established. A RVFCW was installed in a private garden of a ve-member household in Midreshet Ben Gurion
(30 510 0400 N and 34 460 5700 E; the Negev desert, Israel) to
water a 150 m2 garden. The familys expected GW ow
was about 450 l d1. The system reused GW originating
from laundry, showers and sinks. The GW owed through
a 40 l sedimentation tank to the root zone in the VFCW
unit and trickled into a 500 l reservoir. The surface area
of the VFCW was determined to be 1 m2 following the
model of Kadlec and Knight (1996) assuming a conservative average retention time of 8 h. The bed depth was
0.5 m and the recycling rate was 390 l h1 or, in other
words, the water passed through the RVFCW bed between
7 and 21 times (824 h retention time per 450 l daily ow at
a recycling rate of 390 l h1). The treated water overowed
to a secondary 40 l settling tank with submerged pump that
was controlled by a oat. Whenever this tank was full,
water was pumped for irrigation.
After establishment for 3 months, samples from the sedimentation tank and at the outlet were taken periodically
and analyzed for the aforedescribed parameters for over
12 months.
Data on the study areas climatic conditions were supplied by the Israeli Meteorological Service. The mean daily
maximum and minimum temperatures SD were 29.2
4 C and 16.5 3 C, respectively; the mean daily maximum and minimum relative humidities were 93 6% and
39 11%, respectively. Daily maximum global radiation
varied from 600 to 1054 (Vat m2), and direct radiation
from 370 to 1012 (Vat m2).
2.3. Feasibility analysis
A feasibility analysis of the RVFCW was conducted for
the long-term case study. Values for capital costs, maintenance, operation and energy were based on real costs.
The average irrigation costs (assuming irrigation with
freshwater) or in other words, the potential savings, was
estimated according to average water use and local water
costs, and other indirect costs such as infrastructure taxes
that are based on water consumption. Based on these costs
and savings, the return over investment (ROI) was calculated. The analysis was conducted with the assistance of
Ad-Wiser economic consulting oce (Ramat Gan, Israel).
3. Results and discussion
3.1. RVFCW design criteria
Typically, the design parameters for the VFCW are:
required area, depth, media and retention times, and the
main one, BOD of the euent (Vymazal et al., 1998). Interestingly, although there are a few models for sizing VFCW,
experience-based rules of thumb are often applied for
wetland design (Vymazal et al., 1998). The following model
(Eq. (1)) suggested by Kadlec and Knight (1996) was used
to determine the area of the RVFCW, taking into consideration that the euent passes the wetland several times:
A
Q
C i C
xLn
k
C e C
The k value for BOD was previously estimated for lowquality GW as 0.16 m d1 (data not shown). The target
BOD concentration (Ce) was chosen as 10 mg l1 according
to the Israeli guidelines for euent quality for use in cities
(Halperin and Aloni, 2003). Based on that, for a water ow
of 500 l d1 (family of ve) and a recirculation rate of
about 400 l h1, the area was calculated to be approximately 1 m2. The depth of the suggested RVFCW was
0.5 m, within the range typically recommended (0.5
0.8 m) for VFCW (Cooper et al., 1996; Vymazal et al.,
1998).
The contaminated GW is introduced into the root
zone in the VFCW unit, which prevents bad odors and
mosquitoes, and reduces the possibility of human contact
and spread of disease. From the root zone the water falls
through the porous media to the collection reservoir,
enhancing aeration (similar to trickling lter principles),
which further prevents the development of odors and
enhances organic matter degradation and nitrication.
The lime pebbles buer the euents natural acidity, as well
as that produced by nitrication and biodegradation.
Recycling the water from the reservoir back to the upper
lter serves to dilute new raw GW and reduces the risk
of organic overload or other damage to the lter, such as
excess chlorination. Moreover, since the water passes
through the lter more than once, the area required to
attain a specic water quality is reduced. In small commu-
919
Table 1
Average concentrations standard errors (SE) and the ranges of the greywater (GW), treated GW, and freshwater used to irrigate lettuce plants
Analysis
GW
Treated GW
TSS
158 30
85285
466 66
280688
839 47
702984
22.8 1.8
17.227
34.3 2.6
25.045.2
0.3 0.1
0.10.5
0.3 0.2
0.01.0
3.0 1.3
0.05.8
1.2 0.1
1.01.3
6.37.0
7.9 1.7
4.715.6
1.6 0.1
1.41.7
5 107 2 107
9 1041 108
31
06
0.7 0.3
01.5
157 62
60220
6.6 1.1
3.510.2
10.8 3.4
1.421.0
0.9 0.7
0.04.5
0.2 0.2
0.00.9
8.6 4.3
0.023.5
1.3 0.1
1.11.4
7.08.0
0.6 0.1
0.41.3
0.6 0.1
0.40.8
2 105 1 105
3 1027 105
BOD
COD
TP
TN
Total ammonia
nitrogen (TAN)
NO2N
NO3N
EC (dS m1)
PH
Anionic
Surfactants
Boron
FC (CFU 100 ml1)
Mean % removal
98
100
81
71
69
8
92
Freshwater
Standards
10a
0.5 0.1
0.40.6
10a
0.08 0.00
0.020.13
5.7 1.5
47.3
0.1 0.1
00.2
<0.05
8b
2b
4.9 0.7
4.15.7
1.2 0.1
1.21.3
7.18.8
65
0.30 0.03
0.5b
99
<1
< 1a
The GW was treated with recycled vertical ow constructed wetland (RVFCW) and the mean percent removal demonstrates its performance. Samples
were taken twice a month between march and may 2002 (n = 6), and results are in mg l1 unless otherwise.
a
Standard applies to the Israeli guidelines for euent quality for use in cities (Halperin and Aloni, 2003).
b
Standards from the Israeli Water Law (1975) for unlimited use (except drinking).
100
75
75
50
25
TSS
COD
BOD
% Removal
% Removal
920
50
25
Total phosphorus
Total nitrogen
0
0
25
50
75
100
25
50
100
108
100
107
Fecal coliforms
Total Coliforms
106
75
% Removal
75
50
25
Boron
Anionic surfactants
105
104
103
102
101
100
25
50
75
100
Time (hours)
25
50
75
100
Time (hours)
Fig. 2. Percent removal of: total suspended solids (TSS), 5-day biological oxygen demand (BOD), chemical oxygen demand (COD), total phosphorus,
total nitrogen, boron, anionic surfactant, fecal coliforms and total coliforms over time by a RVFCW. The greywater was recycled through the lter
continuously at a rate of 390 l h1. Initial values (time zero) as in GW Table 1.
high-quality water for landscape irrigation. If surface irrigation is used, a disinfection method should be considered.
3.2.2. Batch greenhouse study
The experimental set-up was designed to test the performance of the RVFCW over a longer period of time and to
emphasize the dierent eects of treated GW as compared
to freshwater and untreated GW on the soil and plants. In
addition, the experiment was used to compare the fertilization properties of the dierent sources. The average
water quality and ranges of the dierent sources is summarized in Table 1. The average TSS, BOD, and FC were
158 mg l1, 466 mg l1 and 5 107 CFU 100 ml1, respectivelymuch higher than current standards for very high
quality treated wastewater that can be used for irrigation
in cities (Halperin and Aloni, 2003). Two parameters were
used to evaluate the RVFCW treatment system: (a) its ability to remove variables of environmental and health concern, and (b) whether the output water met current
Israeli standards as already described (Table 1). Concentrations of minerals and metals were within acceptable limits
for reuse and were not signicantly dierent (p < 0.05)
between sources (data not shown). The treated GW met
the current standard except with regard to the FC count,
which decreased by an average two orders of magnitude
from 107 to around 105 CFU 100 ml1. Results were simi-
-1
TSS (mg l )
921
450
375
300
225
150
75
Guidelines for
unlimitted irrigation
Inlet
20
Outlet
10
0
Inlet
300
1
OC (%
dry soil)
TN (mg kg
dry soil)
pH
FC (CFU g
dry soil)
GW
Treated GW
Freshwater
0.29 0.02
0.26 0.01
0.22 0.01
385 35
176 18
70 7
8.2
8.5
8.6
-1
100
20
Guidelines for
unlimitted irrigation
Outlet
10
0
106
-1
Source
BOD5 (mg l )
1
200
Table 2
Average ( standard error) concentrations of organic carbon (OC), total
nitrogen (TN), pH, and fecal coliform count (FC) in sandy soil following 3
months irrigation with freshwater, greywater (GW) and treated GW.
Source water quality as in Table 1
Table 3
Average growth parameters per lettuce head following the dierent
treatments (weight, number of leaves, and surface area) and average
concentration of boron (B) and chloride (Cl) in the lettuce leaves from
the dierent treatments, after 3 months of irrigation (n = 6)
Irrigation
source
Greywater
Treated GW
Freshwater
Initial
13.3
3.5b
2.2c
0.2c
Number
Surface
area (cm2)
35.7
17.5b
12.4c
6c
1200
260b
166c
55c
Cl in
leaves
(mg kg1
dry leaf)
127
84b
65b
39c
B in leaves
(mg kg1
dry leaf)
105
104
0.5
0.2b
0.2b
0.2b
a,b,c
Values in a column followed by dierent letters dier signicantly at
p < 0.05.
Guidelines for
unlimitted irrigation
103
102
101
Outlet
100
Mar-03
Inlet
Jun-03
Sep-03
Dec-03
Mar-04
Date
Fig. 3. The total suspended solids (TSS), 5-day biochemical oxygen
demand (BOD), and fecal coliforms concentration over time in raw and
treated greywater (GW). Greywater was treated with recycled vertical ow
constructed wetland (RVFCW). The solid line represent the allowed
standard concentration for irrigation in cities (Halperin and Aloni, 2003).
922
923
Spackman, R., Entry, J.A., Sojka, R.E., Ellsworth, J.W., 2003. Polyacrylamide for coliform bacteria removal from agricultural wastewater.
J. Soil Water Conserv. 58, 276283.
Vymazal, J., Brix, H., Cooper, P.F., Habrel, R., Perer, R., Laber, J.,
1998. Removal mechanisms and types of constructed wetlands. In:
Vymazal, J., Brix, H., Cooper, P.F., Green, M.B., Habrel, R. (Eds.),
Constructed Wetlands for Wastewater Treatment in Europe. Backhuys
Publishers, Leiden, The Netherlands, p. 366.
Wiel-Shafran, A., Ronen, Z., Weisbrod, N., Adar, E., Gross, A., 2006.
Changes in soil properties following irrigation with surfactant-rich
greywater. Ecol. Eng. (in press).