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plant growth. Thus, fermented fish waste could be used as a valuable organic liquid fertilizer for better yield
from crops at lesser cost and also without the harmful effects of chemical fertilizers.
Article History
Received :
Revised :
Accepted :
1. Introduction
10.04.2015
16.05.2015
22.05.2015
E-mail: jayalakshmichand26@gmail.com
S. Suganthi/Life Science Archives (LSA), Volume 1, Issue 3, Page 192 to 195, 2015
2. Materials and Methods
Preparation and fermentation of fish waste
Fifty g of fish waste was collected from
local market and boiled with 1000 ml distilled
water for 5 minutes and allowed to stand for 30
minutes. The extract was then filtered using
muslin cloth and stored. The extract thus obtained
was taken for fermentation by the addition of
Yeast (1%) and molasses (10%) at room
temperature for a time period of 7 days (Doss et
al, 2008). The fermented fish waste was recovered
by centrifugation at 6000 rpm, 20 min and was
sterilized at 121C for 15 min.
Seed Germination Procedure
The seeds were washed with tap water and
then surface sterilized using 2% Sodium
hypochlorite for 2 minutes. Again the seeds were
washed with distilled water 2 - 5 times and to
check the effect of the product on plant growth,
the seeds were soaked in the fermented fish waste.
Petriplates were sterilized by keeping blotting
papers inside them. The seeds were transferred to
the petriplates and the moisture content was
maintained. The result was observed after 4 - 5
days (Mosha, 1983).
Plant growth promotion using fermented fish
waste cup assay
The germinated seeds were sowed in soil
treated with different concentration (1, 10, 20, 40
and 50 %) of the fermented fish waste in plastic
cups and the effect of the product in plant growth
promotion was studied. The moisture content was
maintained by sprinkling water on a daily basis.
After 7 days of growth, the plantlets were
carefully removed and evaluated for plant growth
parameters.
Evaluation of plant growth parameters
Bulk production
The germinated seeds were sowed in soil
treated with different concentration (1, 10, 20, 30,
40 and 50 %) of fermented fish waste in plastic
193
S. Suganthi/Life Science Archives (LSA), Volume 1, Issue 3, Page 192 to 195, 2015
194
No. of
seeds used
10
10
10
10
10
10
10
No. of seeds
germinated
4
6
8
8
9
9
10
%
Germination
40
60
80
80
90
90
100
Root length
(cm)
10.3
13.2
14.5
15.2
15.5
16
16.9
Shoot
length (cm)
11.9
14.3
15.6
16.2
16.9
17.9
19.2
No. of
hairy roots
9
12
18
20
22
24
39
No. of
leaves
2
2
2
2
2
2
2
Parameter
Total Chlorophyll
Protein
Reducing sugars
Total phenols
Composition (mg/ml)
7.74
12.54
1.16
57.45
5. References
4. Conclusion
S. Suganthi/Life Science Archives (LSA), Volume 1, Issue 3, Page 192 to 195, 2015
195