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Article history:
Received 8 September 2014
Received in revised form
17 December 2014
Accepted 30 December 2014
Available online 8 January 2015
Mixture modeling methodology was used to investigate interactions of sugar, oligofructose and inulin in
papaya nectars as related to sensory liking and chemical characteristics. Mixing sugar and inulin and
increasing the sugar proportion raised the liking of avor and sweetness and the overall acceptability of
papaya nectars. Addition of the three components, along with raising the sugar proportion, increased the
ash and soluble solids content in papaya nectars. The internal preference mappings showed that all
nectars with oligofructose and inulin were as well liked as nectar containing sugar alone, except for some
formulations with lower quantities of sugar. Formulations with 6 g/100 g sugar and 6 g/100 g inulin, or
with 8 g/100 g sugar, 2 g/100 g inulin and 2 g/100 g oligofructose, can be considered to be the best
formulations to produce, with regard to sensory liking and adequacy of chemical parameters, besides all
papaya nectars with addition of oligofructose and inulin can potentially be claimed as prebiotic.
2015 Elsevier Ltd. All rights reserved.
Keywords:
Oligofructose
Inulin
Hedonic scale
Internal preference mapping
1. Introduction
Prebiotics as functional foods have been widely researched
because of the benets that are promoted. These include stimulation of the proliferation or activity of desirable bacterial populations in the colon, such as bidobacteria and lactobacilli
(probiotics), with consequent fermentation that produces lactic
acid, short chain fatty acids (acetic, propionic and butyric acids) and
gases, thereby reducing the intestinal pH and inhibiting proliferation of harmful microorganisms (Wang, 2009).
The main prebiotics currently available and used by the food
industry worldwide belong to the group of carbohydrates, and
specically the category of bers. Among these, isomaltooligosaccharides,
trans-galacto-oligosaccharides,
fructo, Ka
polna, Ka
polna,
oligosaccharides and inulin can be cited (Siro
& Lugasi, 2008). The latter two are the ones that have been most
studied and are the ones still to be approved by the Brazilian National Agency for Sanitary Surveillance (ANVISA, 2013) with regard
H.F. Braga, A.C. Conti-Silva / LWT - Food Science and Technology 62 (2015) 854e860
Table 1
Simplex centroid design and sugar (X1), oligofructose (X2) and inulin (X3) levels in
papaya nectar.
Formulation
1
2
3
4
5
6
7
8
9
10
11
a
Component proportiona
X1
X2
X3
Sugar
Oligofructose
Inulin
1
0
0
0.5
0.5
0
0.33
0.33
0.66
0.17
0.17
0
1
0
0.5
0
0.5
0.33
0.33
0.17
0.66
0.17
0
0
1
0
0.5
0.5
0.33
0.33
0.17
0.17
0.66
12
0
0
6
6
0
4
4
8
2
2
0
12
0
6
0
6
4
4
2
8
2
0
0
12
0
6
6
4
4
2
2
8
X1 X2 X3 1.
855
856
H.F. Braga, A.C. Conti-Silva / LWT - Food Science and Technology 62 (2015) 854e860
(cases), and the data were standardized before analysis. The PCA
was performed with a correlation matrix and without factor rotation. Percentage variation greater than 70% explained by the two
rst principal components indicates strong correlation among
variables and that PCA is an appropriate multivariate analysis to be
applied to the data (Mardia, Kent, & Bibby, 1979).
3. Results and discussion
3.1. Sensory acceptability of papaya nectars
Fifty-nine percent of the consumers mentioned that they
consumed fruit nectar at least twice a week and 14% once a week;
and 100% of the consumers indicated that they liked papaya: 33%
were very fond of it, 58% like it moderately and 9% only a little.
The ranges of sensory acceptability of the papaya nectars were:
7.0 to 8.0 for appearance, 5.9 to 7.0 for aroma, 5.7 to 7.1 for viscosity,
4.8 to 6.7 for avor, 4.4 to 7.1 for sweetness and 5.0 to 6.8 for overall
acceptability.
Regarding to liking of appearance, aroma and viscosity, all the
models did not show any lack of t (p > 0.05), but were not signicant (p > 0.05), thus indicating that the three components did
not have any effect on these dependent variables.
The liking of the avor and sweetness and the overall acceptability were inuenced by the three individual components and the
interaction between sugar and inulin (Table 2). Since the components showed the same effects in relation to the three dependent
variables and the models are very similar (Table 2), only one
triangular diagram is shown, but the results and discussion are the
same for the three variables. The triangular diagram showed that
increasing the proportion of sugar increased the liking of avor and
sweetness and the overall acceptability of the papaya nectars,
although in the same region of the response surface, the mixture of
sugar and inulin resulted in high liking for the nectars (Fig. 1). On
the other hand, the increment on proportions of inulin or oligofructose decreased the liking of all sensory variables.
Inulin and oligofructose have different technological characteristics. Inulin is an amorphous white powder, with a neutral odor
and tastes (Franck & De Leenheer, 2005), while oligofructose has
functional qualities similar to those of sucrose or glucose syrup
(Roberfroid, 2007) and may provide 30e50% of the sweetness of
sugar (Kaur & Gupta, 2002). Thus, high concentrations of inulin or
oligofructose may interfere negatively with the sensory liking of
nectars, through either low or high sweetness, respectively. In
another study, around of 37% of the consumers reported having
noticed a change in the taste or the presence of a residual taste in
the throat when peach nectar with inulin, at a concentration of 2 g/
100 mL of nectar, was compared with another peach nectar without
inulin (Pimentel, Prudencio, & Rodrigues, 2011). Nectar with caja
(Spondians mombin L.) and cashew (Anacardium occidentale)
enriched with inulin with a high degree of polymerization was less
Table 2
Models and goodness of t for the dependent variables.
Dependent variable Equationa
Flavor
Sweetness
Overall
acceptability
Ash (g/100 g)
Soluble solids
( Brix)
a
R2
(%)
p-value Lack
of t
0.654
0.741
0.085
83.3 0.024
0.677
68.6 0.079
0.967
H.F. Braga, A.C. Conti-Silva / LWT - Food Science and Technology 62 (2015) 854e860
857
858
H.F. Braga, A.C. Conti-Silva / LWT - Food Science and Technology 62 (2015) 854e860
Fig. 2. Dendograms resulting from cluster analysis (A, C) and internal preference mapping (B, D) for papaya nectars. Legend: 1 12 g/100 g sugar; 2 12 g/100 g oligofructose;
3 12 g/100 g inulin; 4 6 g/100 g sugar/6 g/100 g oligofructose; 5 6 g/100 g sugar/6 g/100 g inulin; 6 6 g/100 g oligofructose/6 g/100 g inulin; 7-8 4 g/100 g sugar/4 g/100 g
oligofructose/4 g/100 g inulin; 9 8 g/100 g sugar/2 g/100 g oligofructose/2 g/100 g inulin; 10 2 g/100 g sugar/8 g/100 g oligofructose/2 g/100 g inulin; 11 2 g/100 g sugar/2 g/
100 g oligofructose/8 g/100 g inulin. Numbers I to IV indicate the quadrants of the graphs.
liking for all the sensory attributes, and for overall acceptability,
since it was positioned further away from the zero point in the two
principal components and on the same side of the vectors relating
to sensory acceptability.
The second main component, characterized as chemical characteristics was explained by the ash, soluble solids and total solids
(Fig. 4A). It was observed that the formulations containing 12 g/
100 g sugar, 4 g/100 g of each component and 8 g/100 g sugar, 2 g/
100 g inulin and 2 g/100 g oligofructose (formulations 1, 7-8 and 9,
respectively) were characterized by their ash, soluble solid and
total solids content. This result coincided with the triangular diagrams for the chemical characteristics (Fig. 3), as the darkest band,
and this corresponded to the highest values for ash and soluble
solids, comprising the formulations described above.
Combining the three statistical analyses used in this work,
mixture modeling methodology, internal preference mapping and
principal component analysis, is possible to reach conclusions
about different samples and dependent variables. Papaya nectar
with sugar alone (formulation 1), or with 6 g/100 g sugar and 6 g/
100 g inulin (formulation 5) or even with 8 g/100 g sugar, 2 g/100 g
inulin and 2 g/100 g oligofructose (formulation 9), are located in the
region of high sensory liking in the triangular diagrams for avor,
sweetness and overall liking (Fig. 1). These formulations also can be
H.F. Braga, A.C. Conti-Silva / LWT - Food Science and Technology 62 (2015) 854e860
859
4. Conclusions
The interactions among sugar, oligofructose and inulin inuence
the sensory acceptability and chemical characteristics of the papaya
nectars. Increasing the sugar proportion and mixing sugar and
inulin raise the liking of avor and sweetness and the overall
acceptability of the papaya nectars. Addition of the three components, just like increasing the sugar proportion, increases the ash
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