Description

ProductSheet

CCD841CoN(ATCCCRL
1790)
PleasereadthisFIRST

StorageTemp.
liquidnitrogen
vaporphase

BiosafetyLevel
1

Organism:Homosapiens,human
Tissue:colon
Disease:normal
Age:21weeksgestationfetus
Gender:female
Morphology:epithelial
GrowthProperties:adherent
DNAProfile:
Amelogenin:X
CSF1PO:10,11
D13S317:11,13
D16S539:10,11
D5S818:12,13
D7S820:11
THO1:7,8
TPOX:9,10
vWA:14,18
CytogeneticAnalysis:Thelineisdiploidandnoconsistentmarkerchromosomeswereobserved.

BatchSpecificInformation
RefertotheCertificateofAnalysisforbatchspecifictestresults.

IntendedUse

SAFETYPRECAUTION
Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.

CompleteGrowthMedium
ThebasemediumforthiscelllineisATCCformulated
Eagle'sMinimumEssentialMedium,CatalogNo.30
2003.Tomakethecompletegrowthmedium,addthe
followingcomponentstothebasemedium:fetalbovine
serumtoafinalconcentrationof10%.

ATCChighlyrecommendsthatprotectiveglovesandclothingalwaysbeusedandafullfacemaskalwaysbe
wornwhenhandlingfrozenvials.Itisimportanttonotethatsomevialsleakwhensubmersedinliquidnitrogen
andwillslowlyfillwithliquidnitrogen.Uponthawing,theconversionoftheliquidnitrogenbacktoitsgas
phasemayresultinthevesselexplodingorblowingoffitscapwithdangerousforcecreatingflyingdebris.

Unpacking&StorageInstructions
1. Checkallcontainersforleakageorbreakage.
2. Removethefrozencellsfromthedryicepackagingandimmediatelyplacethecellsatatemperature
below130C,preferablyinliquidnitrogenvapor,untilreadyforuse.

CitationofStrain

HandlingProcedureforFrozenCells
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:CCD841CoN(ATCCCRL1790)

AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org
800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

Page1of3

Toinsurethehighestlevelofviability,thawthevialandinitiatethecultureassoonaspossibleuponreceipt.If
uponarrival,continuedstorageofthefrozencultureisnecessary,itshouldbestoredinliquidnitrogenvapor
phaseandnotat70C.Storageat70Cwillresultinlossofviability.
1. Thawthevialbygentleagitationina37Cwaterbath.Toreducethepossibilityofcontamination,keep
theOringandcapoutofthewater.Thawingshouldberapid(approximately2minutes).
2. Removethevialfromthewaterbathassoonasthecontentsarethawed,anddecontaminateby
dippinginorsprayingwith70%ethanol.Alloftheoperationsfromthispointonshouldbecarriedout
understrictasepticconditions.
3. Transferthevialcontentstoacentrifugetubecontaining9.0mLcompleteculturemediumandspinat
approximately125xgfor5to7minutes.Discardsupernatant.
4. Resuspendthecellpelletwiththerecommendedcompletemediumanddispenseintoa25cm2culture
flask.Itisimportanttoavoidexcessivealkalinityofthemediumduringrecoveryofthecells.Itis
suggestedthat,priortotheadditionofthevialcontents,theculturevesselcontainingthecomplete
growthmediumbeplacedintotheincubatorforatleast15minutestoallowthemediumtoreachits
normalpH(7.0to7.6).
5. Incubatethecultureat37Cinasuitableincubator.A5%CO2inairatmosphereisrecommendedif
usingthemediumdescribedonthisproductsheet.

HandlingProcedureforFlaskCultures
Theflaskwasseededwithcells(seespecificbatchinformation)grownandcompletelyfilledwithmediumat
ATCCtopreventlossofcellsduringshipping.
1. Uponreceiptvisuallyexaminethecultureformacroscopicevidenceofanymicrobialcontamination.
Usinganinvertedmicroscope(preferablyequippedwithphasecontrastoptics),carefullycheckfor

anyevidenceofmicrobialcontamination.Alsochecktodetermineifthemajorityofcellsarestill
attachedtothebottomoftheflaskduringshippingtheculturesaresometimeshandledroughlyand
manyofthecellsoftendetachandbecomesuspendedintheculturemedium(butarestillviable).
2. Ifthecellsarestillattached,asepticallyremoveallbut5to10mLoftheshippingmedium.The
shippingmediumcanbesavedforreuse.Incubatethecellsat37Cina5%CO2inairatmosphere

ProductSheet

CCD841CoN(ATCCCRL

untiltheyarereadytobesubcultured.
3. Ifthecellsarenotattached,asepticallyremovetheentirecontentsoftheflaskandcentrifugeat
125xgfor5to10minutes.Removeshippingmediumandsave.Resuspendthepelletedcellsin10
mLofthismediumandaddtoa25cm2flask.Incubateat37Cina5%CO2inairatmosphereuntilcells

1790)

arereadytobesubcultured.

SubculturingProcedure
PleasereadthisFIRST

StorageTemp.
liquidnitrogen
vaporphase

BiosafetyLevel
1

IntendedUse
Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.

Volumesaregivenfora75cm2flask.Increaseordecreasetheamountofdissociationmediumneeded
proportionallyforculturevesselsofothersizes.
1. Removeanddiscardculturemedium.
2. BrieflyrinsethecelllayerwithCa++/Mg++freeDulbecco'sphosphatebufferedsaline(DPBS)or
0.25%(w/v)Trypsin0.53mMEDTAsolutiontoremovealltracesofserumwhichcontainstrypsin
inhibitor.
3. Add1.0to2.0mLofTrypsinEDTAsolutiontoflaskandobservecellsunderaninvertedmicroscope
untilcelllayerisdispersed(usuallywithin5to15minutes).
Note:Toavoidclumpingdonotagitatethecellsbyhittingorshakingtheflaskwhilewaitingforthe
cellstodetach.Cellsthataredifficulttodetachmaybeplacedat37Ctofacilitatedispersal.
4. Add6.0to8.0mLofcompletegrowthmediumandaspiratecellsbygentlypipetting.
5. Transfercellsuspensiontoacentrifugetubeandspinatapproximately125xgfor5to10minutes.
Discardsupernatant.
6. Resuspendthecellpelletinfreshgrowthmedium.Addappropriatealiquotsofthecellsuspensionto
newculturevessels.
7. Incubateculturesat37C.
SubcultivationRatio:Asubcultivationratioof1:2to1:3isrecommended
MediumRenewal:Twiceperweek

CompleteGrowthMedium

CryopreservationMedium
ThebasemediumforthiscelllineisATCCformulated
Eagle'sMinimumEssentialMedium,CatalogNo.30
2003.Tomakethecompletegrowthmedium,addthe
followingcomponentstothebasemedium:fetalbovine
serumtoafinalconcentrationof10%.

CitationofStrain
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:CCD841CoN(ATCCCRL1790)

Completegrowthmediumdescribedabovesupplementedwith5%(v/v)DMSO.
CellculturetestedDMSOisavailableasATCCCatalogNo.4X.

Comments
Morphologicallythecellsresembleepithelialcellshowever,thecellsdonotcontainkeratinanddefinitive
evidenceofepithelialoriginislacking.

References
Referencesandotherinformationrelatingtothisproductareavailableonlineatwww.atcc.org.

BiosafetyLevel:1
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Laboratorysafetyisdiscussedin
thecurrentpublicationoftheBiosafetyinMicrobiologicalandBiomedicalLaboratoriesfromtheU.S.
DepartmentofHealthandHumanServicesCentersforDiseaseControlandPreventionandNationalInstitutes
forHealth.

ATCCWarranty

AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org
800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

TheviabilityofATCCproductsiswarrantedfor30daysfromthedateofshipment,andisvalidonlyifthe
productisstoredandculturedaccordingtotheinformationincludedonthisproductinformationsheet.ATCC
liststhemediaformulationthathasbeenfoundtobeeffectiveforthisstrain.Whileother,unspecifiedmedia
mayalsoproducesatisfactoryresults,achangeinmediaortheabsenceofanadditivefromtheATCC
recommendedmediamayaffectrecovery,growthand/orfunctionofthisstrain.Ifanalternativemedium
formulationisused,theATCCwarrantyforviabilityisnolongervalid.

Disclaimers
Thisproductisintendedforlaboratoryresearchpurposesonly.Itisnotintendedforuseinhumans.

Page2of3

1790)

WhileATCCusesreasonableeffortstoincludeaccurateanduptodateinformationonthisproductsheet,
ATCCmakesnowarrantiesorrepresentationsastoitsaccuracy.Citationsfromscientificliteratureand
patentsareprovidedforinformationalpurposesonly.ATCCdoesnotwarrantthatsuchinformationhasbeen
confirmedtobeaccurate.
Thisproductissentwiththeconditionthatyouareresponsibleforitssafestorage,handling,anduse.ATCC
isnotliableforanydamagesorinjuriesarisingfromreceiptand/oruseofthisproduct.Whilereasonableeffort
ismadetoensureauthenticityandreliabilityofstrainsondeposit,ATCCisnotliablefordamagesarisingfrom
themisidentificationormisrepresentationofcultures.
PleaseseetheenclosedMaterialTransferAgreement(MTA)forfurtherdetailsregardingtheuseofthis
product.TheMTAisalsoavailableonourWebsiteatwww.atcc.org

PleasereadthisFIRST

AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.
ATCC2015.Allrightsreserved.ATCCisaregisteredtrademarkoftheAmericanTypeCultureCollection.[07/13]

ProductSheet

CCD841CoN(ATCCCRL

StorageTemp.
liquidnitrogen
vaporphase

BiosafetyLevel
1

IntendedUse
Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.

CompleteGrowthMedium
ThebasemediumforthiscelllineisATCCformulated
Eagle'sMinimumEssentialMedium,CatalogNo.30
2003.Tomakethecompletegrowthmedium,addthe
followingcomponentstothebasemedium:fetalbovine
serumtoafinalconcentrationof10%.

CitationofStrain
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:CCD841CoN(ATCCCRL1790)

AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org
800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

Page3of3