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on
line
data
acquisition
&
display;
using
appropriate
4)
Prepare
wiring
sequence
as
per
you
have
perform
experiments.
Step 5)Switch ON Master Unit.
Step 6) Change setting click on setting button following screen shall
be seen on your PC monitor.
Step 7)There are two types of graphi)
ii)
Online Graph
Burst Graph
EXPERIMENT NO.6
AIM: To acquire real time ECG using surface electrode and calculate heart
beat.
APPARATUS REQUIRED : Master unit (Xpo-BOM) , RRM/BIA Experimental
panel, digital multimeter, patch chords ,ECG/EMG experimental panel,
electrodes (limbs).
THEORY:
A. An electrocardiogram (ECG) is a recording of the electrical activity on
the body surface generated by heart.ECG measurement information is
collected by skin electrodes placed at designated locations on the
body. The ECG signal is characterized by six peaks and valleys labeled
with successive letters of the alphabet P , Q , R , S , T , and U.
Experiment configuration is envisaged for use with a personal
computer . Although this panel is made with patient safety in mind,
any ideas presented are not by themselves necessarily compatible with
all system safety requirements.
B. REQUIREMENTS FOR AN ELECTROCARDIOGRAPH :
The front end of an ECG must be able to deal with extremely weak
signals ranging
the
electrode skin
end of the limb (Wrists and ankles) or at the origin of the limb (shoulder or
upper thigh) makes no difference in the recording because the limb can
simply be viewed as a long wire conductor originating from the point on
the trunk of the body.
Based upon universally
heading toward the left arm gives a positive deflection in lead I because
the positive electrode is on the lest arm.Maximal positive ECG deflection
occurs in lead I when a wave of depolarization travels parallel to the axis
between the right and left arms . If a wave of depolarization heads away
from the left arm , the deflection is negative. Also by these rules , a wave
of repolarization moving away from the left arm is recorded as a positive
deflection . Similar statements can be made for leads II and IIIin which
the positive electrode is located on the left leg. For example , a wave of
depolarization traveling toward the left leg produces a positive deflection
in both leads II and III because the positive electrode for both leads is
on the left leg. A maximal positive deflection is recorded in lead II when
the depolarization wave travels parallel to the axis between the right arm
and left leg .Similarly , a maximal positive deflection is obtained in lead
III when the depolarization wave travels parallel to the axis between the
left arm and left leg.
PROCEDURE:
Wiring Sequence :Connect BS5 1-2 , 12 BS5 4,-12-BS5 6 , GND-BS5 5,
BS5-(Top board ADC2 is CH3 or ADC1 is CH2).
1. Use clamp type surface electrodes.
2. Apply Gel to surface electrodes & Connect surface Electrodes 4 nos.to
body as shown in figure &colour coding as mentioned . Insert 4mm
plugs from 9 pin D connector located on left hand side of panel.
3. Switch ON SW4 (Battery P ON, UP position) & Master unit.
4. Confirm SW5 switch is left position to save Battery.
5. Calculate Heart rate as fllows:
HEART RATE =
S1
O.
switch
Betwee Between
1
2
3
position
1
2
3
Lead I
Lead II
Lead
n
RA-LA
RA-LL
LA-LL
LA,RL
RA,RL
LL
-
III
aVR
LALL-
aVL
RA
RALL-
aVF
LA
RALA-
4
5
6
5
6
Rotary LEAD
Signal
Short
Open
LL
CONCLUSION:
1) Shifting of the Baseline: A wandering baseline as seen in adjoining
figure but otherwise normal ECG trace is usually due to the movement
of the patient or electrodes. The baseline shift can be eliminated by
ensuring that the patient lies relaxed& the electrodes are properly
attached.
2) Muscle Tremor: Irregular trembling of the ECG trace in figure A
without wondering of the baseline occurs when the patient is not
relaxed or is cold. It is found in the case of older patients.
3) Interface from the power line: Power line interference is easily
recognizable since the interfacing voltage in the ECG would have a
frequency of 50 HZ. This can be verified by bypassing notch filter &
observe interference.
4) In all the bipolar lead positions ,QRS of a normal heart is such that the
R wave is positive & is generated in lead II.
EXPERIMENT NO. 7
AIM: To acquire real time EMG using surface electrode and study its
characteristics.
MATERIAL REQUIRED :BPM , EMG experimental panel , patch chords , pc
(graph utility software), Limb electrodes.
THEORY: Typically externally measured action potential from a single muscle
fibre produces a characteristic biphasic waveform. Imagine what happens
when a recording is made externally to the muscle in a living subject. Instead
of recording from a single muscle fibre you are recording from thousands of
muscle fibres and instead of recording just next to the fibre you are recording
some distance away through various layers of connective tissue and skin.
This means that instead of a nice clean signal of about 100 mV you get a
much more complex signal of about 5 mV.The actual size of the signal is
highly variable and depends on the thickness of the connective tissue, the
quality of the contact between the electrode and the skin ,the size of the
action potentials .These will vary from experiment to experiment so the size
of an EMG is a largely qualitative measure although it does increases in size
with the activation level of a muscle.
When there is little muscle tension there is virtually zero electrical activity. As
the EMG increases after a short lag the tension increases. A surface electrode
may be used to monitor the general picture of muscle activation, as opposed
to the activity of only a few fibres as observed using a needle. This technique
is used in a number of setting; for example in the physiotherapy clinic ,
muscle activation is mentioned using EMG & patients have an auditory or
stimulus to help them know they are activating the muscle (biofeedback). A
motor unit is defined as on motor neuron and all of the muscle fibree in
innervates. When a motor unit fires , the impulse ( called an action
potential ) is carried down the motor neuron to the muscle. The area where
the nerve contacts the muscle is called the Neuromuscular junction or the
motor end plate. After the action potential is transmitted across the neuro
muscular junction ,an action potential is elicited in all of the innervated
muscle fibers of that particular motor unit.
The sum of all this electrical activity is known as a motor unit action potential
(MUAP). This electro physiologic activity from multiple motor units is typically
evaluated during an EMG.
PROCEDURE:
Wiring Sequence : Connect BS5 1-2 , 12 BS5 4,-12-BS5 6 , GND-BS5 5,
BS5-(Top board ADC1 is CH3 or ADC1 is CH2).
1. Use clamp type surface electrodes
2. Apply Gel to surface electrodes & Connect surface Electrodes 3 nos.to
body as shown in figure&colourcoding as mentioned. Insert 4mm plugs
from 9 pin D connector located on left handside of panel.To left forearm
3.
4.
5.
6.
7.
CONCLUSION:
a) EMG provides good semi quantitative data on muscle use.
b) A 3 electrode system is necessary to cancle out background noise.
c) Linear envelope processing with a 50 (60) HZ Notch filter is a good
processing option.
d) There is a variable (-50 ms) time delay between the electrical activity
and tension.
RESULT:
The EMG wavelength from armmuscle have been studied.
EXPERIMENT NO. 8
AIM: To acquire real time EOG using surface electrodes and study its
characteristics.
APPARATUS REQUIRED: Ag/AgCl electrodes, Xp-BOM, EOG experimental
panel, patch chord, PC (graph utility software).
THEORY:
The electrooculogram signals or simply EOG represent the eye balls
movements. The waveform contains useful information about the movement
of the eye balls and generally it may also represent the overall condition of
the human subject such as the state of drowsiness of the subject. The EOG
signal when extracted and analyzed using computers , are highly useful in
detecting changes in the states of the subject
CONCLUSION:
Evoked potentials elicited by electrical stimulation of the oculomotor nerves
and obtained from the surface electrodes originate in the extraocular
muscles. They do not derive from the eye and thus EOG doesnot provide an
appropriate representation.
EXPERIMENT NO.9
AIM: To measure blood pressure of a subject using automatic BPM.
APPARATUS REQUIRED: Automatic blood pressure machine.
THEORY:
A) DIFFERENCE
BETWEEN
SYSTOLIC
AND
DIASTOLIC
BLOOD
below:
Phase 1(K-1): The appearances of the clear tapping sounds as the
cuff is gradually deflated.The first clear tapping sound is defined as
II.
III.
Phase 3(K-3): The sounds become crisper and louder in K-3 which is
IV.
V.
Phase 1
Phase 2
A
Silence
Phase 3
Phase 4
Phase 5
A soft
Regular
Trapping
Swishing
Crisp
Blowing
Soft HB
sound
sound
Sound
sound
Sound
(Begin sound)
(Peak sound)
180
120
110
100
90
80
mmHg
Peak pressure
Systolic
Diastolic
pressure Drop
Pressure
pressure
Fig.9.1: Blood Pressure levels
D) CATEGORIES FOR BLOOD PRESSURE LEVELS IN ADULTS: (IN
mmHg , millimeters of mercury)
TABLE 4.1: BLOOD PRESSURE LEVELS
S.N
CATEGORY
O.
1
2
Normal
Prehypertensio
3a
n
High
SYSTOLIC
DIASTOLIC
( TOP NUMBER)
(BOTTOM
NUMBER)
Less than 80
80-89
Blood 140-159
90-99
Pressure
3b
Stage 1
High
100 or higher
Pressure Stage
2
PROCEDURE:
A) CALIBRATION
OF
ELECTRONIC
PRESSURESENSOR
USING
SPHYGMAMANOMETER
Wiring Sequence: Connect BS5 +12-1,-12-3, GND-2, 11-9,
12-10, 13-DMM.
1. One end of Sphygmomanometer is connected to T Socket while one of
the other two ends is connected to pressure sensor located on the
underside of BMI panel &second goes to wrappable pressure pad.
2. Keep SW3 lower position (down) for low gain of instrumentation OPAmp.
3. Switch ON power supply of master unit.
4. Connect DMM to BS5 no.13.
5. Set 0V by using zero pot(P5) at BS5-13 when no pressure is applied to
pressure pad.
6. Create 100 mmHg pressure by using Sphygmamomanometer/Blood
Pressure module. (Need not wrap)
7. Set 100mV on DMM at BS5 13 & GND.
8. Repeat this procedure two times & observe reading by applying
different pressure in between.
B) BLOOD PRESSURE MEASUREMENT USING KOROTKOFFS
Wiring
Sequence:
Connect
BS5
+12-1,-12-3,GND-2,14-I/P
Preamplifier (on Hind plate of MU), O/P of amplifier (on Hind plate of MU)15.16-I/P L/S (on Hind plate of MU), CH1 or CH2 on TB-O/P L/S (on Hind
plate of MU)
1. One end of Sphygmomanometer is connected o T- socket while one of
the other two ends is connected to pressure sensor located on the
underside of BM 1 panel & other two goes to wrap able pressure pad.
2. Keep SW3 lower position for low gain of instrumentation OP-Amp.
figure.
Switch ON power supply of master unit.
Connect headphone on hind plate of headphone socket.
Headphone placed on the ear.
Increase the pressure by using hand pump nearly equal to 210 mmHg
Hear the last peak sound of pulses (i.e. Korotkcoff sound K-5) at that
time press Hold button on DMM & note down that reading as Diastolic
pressure (Diastolic pressure foe normal person is 80 mmHg.
DIASTOLIC PRESSURE =
------------------------mmHg.
CONCLUSION: You need to do some practice to pick up sound of heart beat
using electret MIC as in the din of various noises around you:the faint sound
of heart is difficult to hear. Correct placement of ear is important.
EXPERIMENT NO. 10
AIM:To measure the blood glucose level using electronic glucometer.
APPARATUS REQUIRED: ACCU-Check.
THEORY:
ACCU-check
active
blood
glucose
meter
is
used
foe
quantitative
determination of blood glucose valves in fresh capillary blood by using ACCUcheck active test strips.
FEATURES:
1) FAST TESTING: The meter needs only approximate 5 seconds for each
testing.
2) EASY TESTING: The person need not to press a single for testing just
insert a test strip into meter, which switches it as apply blood to test
strip , read the valve and then pull test strip out of the meter.
3) BLOOD VOLUME CHECKING: To preform test, the meter requires 1-2 l
blood. The meter requires 1-2 l blood is sufficient or not.
4) FLAGGING RESULT: We can flag results with different symbols, which
indicate particular situation during the test.
5) MEMORY: The meter automatically saves upto 350 results with time
and data of the test and any other information i.e. important for the
test.
6) INTEGRATED DATA ANALYSIS: From the stored test results our neter can
calculate over average values for the last 7,14 or 30 days.
7) DATA TRANSFER: The meter has an infrared part enabling us to
download stored test result to a computer.
8) APPLYING BLOOD OUTSIDE THE METER: When the meter prompts us to
apply blood, we can remove the test strip from the meter, apply the
blood and insert it back into the meter.
PROCEDURE:
1) Preparing to test blood glucose.
TO CARRY OUT THE TEST
a) Meter with code strip inserted.
b) The parts of ACCU check active test strips that belong to code
chop.
c) A laming device to collect blood.
TESTING BLOOD GLUCOSE
i) TURNING THE METER ON:
a)Take a test strip from the container and close the container
again gently.
b) Hold the test strip so the arrows pointed on this and the
orange face ( square) upwards.
c) Without bending it,gently push the test strip into the test strip
guide in the direction of arrows.
ii)
PRECAUTIONS:
EXPERIMENT NO.11
AIM:To generate breathing speed waveform using surface electrode.
APPARATUS REQUIRED: Temperature module with T3 connector cord,DSO.
THEORY: