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Received: 25 March 2009 / Accepted: 26 May 2009 / Published online: 12 June 2009
# Springer Science + Business Media, LLC 2009
Introduction
Portulaca oleracea L. (P. oleracea L., purslane) is a
common, herbaceous succulent annual weed, which is
distributed extensively in temperate and tropical regions
worldwide (Radhakrishnan et al. 2001; Garti et al. 1999). It
has been used as a kind of food and medicinal plant for
thousands of years in China. P. oleracea L. is of abundant
nutrition with contents of proteins, carbohydrates, Ca, K,
Zn, and Na (Aberoumand 2008). As a kind of Chinese
traditional medicine, P. oleracea L. is very important
because of its special medical function, and it has been
used traditionally for the treatment of dysentery with
bloody stools and externally for boils and sores, eczema,
erysipelas, and insect and snake bites (Chen et al. 2003;
Zhang et al. 2002; Yazici et al. 2007; Palaniswamya et al.
2004).
Among the effective ingredients of many Chinese
herbal medicines, flavonoids are one of the most
popular compounds in the plant kingdom. Flavonoids
have received much attention because of their richness
in species numbers and effectiveness in reducing blood
lipid, as an anti-oxidative, in assimilating cholesterol,
inhibiting thrombosis, dilating the coronary artery, etc.
(Deng et al. 2008; De Souza et al. 2008; Su et al. 2008;
Shao et al. 2007; Volpi and Bergonzini 2006; Zhang et al.
2007; Keith et al. 2007).
Recently, the development of flavonoids in medical
and food fields becomes a hot research. Flavonoids and
polysaccharides are the most abundant effective components in P. oleracea L. It is reported that seven kinds
of flavonoids were found in P. oleracea L., including
quercetin, kaempferol, myricetin, apigenin, luteolin,
91
1. Chemicals: authentic standard rutin (>97%) was purchased from Guoyao Group of Chemical Reagents Ltd.
(China). All reagents and chemicals used in this work
were of analytical grade. Triple-distilled water was used
throughout the study.
2. Instruments: pressure self-control microwave decomposition system (MDS-2002AT, Shanghai, China),
Auto Science AS-2060B Ultrasonic Cleaner (Tianjin,
China), and Soxhlet extractor (250 mL, Changsha,
China) were used for extraction. High-speed centrifugation (TGL-16C, Shanghai, China) was employed
to accelerate the phase separation process. A
versatile plant pulverizer (FW-100, Beijing, China)
was used to make the plant materials into powder.
A rotary evaporator (RE52CS, Shanghai, China) was
used to concentrate the sample solution. UVVis
spectrophotometer V-530 (JASCO, Japan) was used
for determination.
Extraction of Flavonoids
Before extraction, P. oleracea L. was crushed into powder
by versatile plant pulverizer. The powders of the samples
were degreased by Soxhlet extractor with petroleum ether
until the color of the eluate became colorless. Flavonoids
92
93
94
Extraction time
(min)
Numbers of
the samples
Absorbance of
each sample
Microwave-assisted extraction
Ultrasonic extraction
Soxhlet extraction
Condensing reflux extraction
Marinated extraction
9
60
300
150
2880
5
5
5
5
5
0.390,
0.371,
0.391,
0.378,
0.313,
0.393,
0.373,
0.390,
0.374,
0.316,
0.391,
0.373,
0.389,
0.375,
0.312,
0.387,
0.376,
0.390,
0.373,
0.317,
0.388
0.373
0.388
0.374
0.313
Average extraction
efficiency (mg/g)
RSD % (n=5)
7.1
6.7
7.0
6.8
5.6
0.29
0.48
0.29
0.51
0.70
95
Table 3 Results of L9 (34) orthogonal test
Abs
1
2
3
4
5
6
7
8
60%
60%
60%
70%
70%
70%
80%
80%
1:30
1:40
1:50
1:30
1:40
1:50
1:30
1:40
7
8
9
8
9
7
9
7
40
45
50
50
40
45
45
50
0.289
0.295
0.368
0.373
0.387
0.373
0.310
0.347
9
K1
K2
K3
k1 =K1/3
k2 =K2/3
80%
0.952
1.133
0.964
0.317
0.378
1:50
0.972
1.029
1.048
0.324
0.343
8
1.009
0.975
1.065
0.336
0.325
40
0.983
0.978
1.088
0.328
0.326
0.307
T=3.049
k3 =K3/3
R
0.321
0.061
0.349
0.025
0.355
0.030
0.363
0.037
Recovery Studies
A
B
C
D
60%
1:30
7
40
70%
1:40
8
45
80%
1:50
9
50
96
Sample (g)
Content (mg)
Added (mg)
Found (mg)
Recovery (%)
RSD (%)
0.5008
0.5002
0.5007
0.5007
0.5005
4.6975
4.6919
4.6966
4.6966
4.6947
5.0
5.0
5.1
5.0
5.2
9.8791
9.8255
9.8887
9.8881
9.9516
103.6
102.7
103.8
101.8
101.1
102.6
1.13
Methodology Studies
Precision Test of the Apparatus
Apparatus precision was evaluated by the analysis of a
solution of the sample for ten consecutive times by
ultravioletvisible detector. RSD obtained was 0.22%,
which showed that the precision of the apparatus was
satisfactory.
Repeatability of Analytical Method
To assess the repeatability of the method, five solutions
made by microwave extraction were determined. RSD
obtained was 0.57%, which showed acceptable
repeatability.
Stability Test of the Solution
Stability was evaluated by the analysis a solution of the
sample extracted by microwave every 15 min. RSD
obtained was 0.32%, which indicated that the stability of
the solution had excellent stability in 2 h.
Sample Analysis
Flavonoids from different kinds of P. oleracea L. extracted
by optimized microwave-assisted extraction conditions
were determined by means of UVVis spectrophotometry,
and the results are shown in Table 5.
We could conclude from Table 5 that the extraction
efficiency of flavonoids in P. oleracea L. were different
from different batches, and the flavonoids content in
different parts of P. oleracea L. (root, stem, and leaf)
indicated that the root was rich in flavonoids, followed by
stem and leaf. A comparison of flavonoids content from
P. oleracea L. between degreased and non-degreased
samples was also investigated (seen in Table 5); from the
table, we can see that lipid material had influence on the
detection results, so the samples used were all degreased in
our study.
Concluding Remarks
In the present study, the microwave-assisted extraction has
been established to extract flavonoids from P. oleracea L.
and quantification was performed by means of UVVis
spectrophotometry. The extraction yield of flavonoids by
microwave-assisted extraction is 0.71%. It is higher than
other extractions, which confirms that microwave-assisted
extraction is very suitable for the sample preparation of
Degreased or not
No
Yes
Yes
No
Yes
Yes
Yes
Yes
RSD %
7.16
7.10
9.38
6.82
6.78
11.36
5.12
1.76
0.29
0.61
0.57
0.39
0.30
0.32
0.81
1.54
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