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MONICA ELA1
Innopan, Spanish Centre of Baking Technology. Parc de Gardeny, Edifici H3, 1a pl. 25003 Lleida, Spain
1
Corresponding author. TEL: 0034 691267649;
FAX: 0034 973283849; EMAIL:
monica@innopan.com
ABSTRACT
Sensory evaluation is a powerful tool with a wide range of applications in the bakery
industry. However, it is necessary to establish a complete methodology for the evaluation. In this study, Innopan has proposed a methodology for the sensory analysis of
bread, defining a set of descriptors by setting the appropriate vocabulary for a complete description of bread and closely related products. The protocol outlined
included 46 attributes sorted by sensory groups (17 for visual, nine for odor, 12 for
flavor and eight for texture), evaluating crumb and crust separately. A trained panel
with 10 people has been formed. Once the protocol was established, the validation of
the panel was performed after 1 year of training. Significant differences were found
between different products.
PRACTICAL APPLICATIONS
This work becomes a guide for the sensory analysis of bread. It provides a complete
set of attributes for the description of bread and closely related products and the
optimum procedure for the evaluation. A standard sensory methodology is an interesting tool for the industry in the development of bakery products to achieve not
only the best technological quality but also to consistently meet consumers expectations. Moreover, the use of a standard sensory methodology facilitates communication among research and producer groups.
INTRODUCTION
Bread is a basic food worldwide. It is part of the Mediterranean diet, which has recently been awarded World Heritage
status as a model for healthy eating (UNESCO, United
Nations, Educational, Scientific and Cultural Organization
2010). In Spain alone, there are over 300 different types of
breads, and there is an increasing interest among bakers to
recover and protect this heritage. Sensory evaluation would
not only constitute an important part for the whole quality
assessment of such diverse breads but also identify factors that
could lead to the development of new products. However,
previous reports on parameters to describe a bread are scattered and use different terms, definitions and methodologies
(Lotong et al. 2000; Gmbaro et al. 2002; Battochio et al.
2006; Heino 2006; Heenan et al. 2008; Jensen et al. 2010).
More recently, Hayakawa et al. (2010) have developed a broad
number of parameters concluding with a study of a selected
set of 23 for differentiating the sensory qualities of French
Journal of Sensory Studies 26 (2011) 269277 2011 Wiley Periodicals, Inc.
M. ELA
Development of Protocol
The development of the protocol was conducted in 1 1/2-h
sessions in a two-step process: (I) generation of descriptors
and their definitions, for establishing a general protocol for
wide range of breads and (II) design of a standard method of
evaluation.
Generation of Descriptors and Their Definitions.
During each session, each of the panelists evaluated separately a set of at least three commercial breads. Afterwards, the
group discussed their findings openly with the intervention of
the panel leader as a moderator. Panelists were asked to
mention the attributes they considered important for
descriptive evaluation of bread and their definitions through
open discussion. After each session, the panel leader discussed
with the panelists other attributes and methodologies previously reported in studies of bread, and the group finally
decided whether or not to include them.
Design of a Standard Method of Evaluation. Three
samples were evaluated in each 1 1/2-h session by each panelist. Samples were always coded with a three-digit random
numbers in a randomized and balanced order and assessed at
room temperature (ISO [International Organization for
Standardization] 8589 1998). Sessions were carried out in a
library.
Breads were specifically processed with a distinguishing
feature according to the group of attributes to evaluate in a
session. For instance, bread samples were prepared with different levels of added yogurt for the evaluation of lactic fat
aroma. Panelists were then asked to evaluate the intensity of
this character on a 10-point continuous linear scale of the
descriptors generated under (I) earlier.
Samples were presented to each panelist with crumb, upper
crust and lower crust separately in different flasks, with different sample sizes to achieve the best presentation for the
sample. Different parts of the breads were sampled. Also, different time intervals since baking were tested in order to
choose the best for sensory evaluation.
M. ELA
1,000
580
18
20
0
60
100
50
1,000
580
9
20
0
60
100
40
1,000
580
18
20
5
60
100
35
1,000
580
0
20
0
60
100
25
ficult to produce consistent samples for each loaf over all four
sessions so visual attributes were also assessed upon the same
set of samples for all the panelists. A limited number of
attributes were chosen for the validation test in order to avoid
panelists fatigue. Four attributes were assessed on the whole
loaf (brightness, score width, score depth and score color),
two attributes on a cut piece (convexity and crumb regularity)
and three attributes of flavor/taste (acetic acid, lactic fat, salty)
on a consumed piece.
Statistical analysis was conducted using Fizz 2.5B software
(Couternon, France) (Biosystmes 2010). Analysis of variance (ANOVA) was used to determine if the attributes used to
differentiate the samples differed between panelists. Fishers
least significant difference test was used to detect any statistically significant differences between samples and panelists.
refer to the same sensory concept (ISO [International Organization for Standardization] 1087 1990; ISO 5492 1992;
Giboreau et al. 2007). For some visual attributes, it was necessary to add a graphic on the sensory sheet to improve understanding of the descriptor (Figs. 1 and 2).
Some terms included in the present set of descriptors are
common to recent studies with other bakery products, which
confirm their importance in bread sensory evaluation and
facilitate comparison (Gmbaro et al. 2002; Heino et al.
2003; Shogren et al. 2003; Kihlberg et al. 2004, 2006; Collar
et al. 2005; Carr et al. 2006; Annett et al. 2007). However,
others differ; e.g., the termyeastutilized in the current study
refers to an attribute very similar to other terms such asbeer
or stout used in other reports (Lotong et al. 2000; Hayakawa
et al. 2010; Jensen et al. 2010). These differences enhance the
importance of a standard protocol for bread evaluation.
Intensity in the 10-point linear continuous scales was verbally anchored at the beginning of each session. In the following sessions, as the attributes were repeatedly worked, the
scales were anchored with bread references for those
attributes difficult to consensus, when possible (Table 2). The
inclusion of such references on the scales is very valuable,
especially among the texture attributes (Romero del Castillo
et al. 2008). However, this cannot be done in the short term
and should only be done with products well known to the
panel.
Finally, the procedure for the evaluation was agreed by the
entire panel. The panel defined the optimum moment of
evaluation as 810 h after baking, at room temperature. The
evaluation was conducted on samples from the crumb, upper
crust and lower crust separately as it has been reported that
they have different flavor properties (Kirchoff and Schieberle
2001; Heino 2006; Hayakawa et al. 2010). The adequate
sample vessel was a standard olive oil flask covered by a watch
glass (ISO [International Organization for Standardization]
16657 2007). The best presentation of the samples for proper
perception of sensory properties was defined as four pieces
(2 2 cm) taken from the central part of the product, sliced
(15-mm-thick slices) and cut with scissors. General features
of the developed methodology are summarized in Table 3.
The first step in the sensory evaluation was defined as the
testing of aroma properties. Panelists must handle the glass
with both hands to warm it in order to facilitate the perception of aromatics wherever room temperature was. The time
was not limited. After assessing aromatic attributes, the same
samples were assessed for the taste and flavor attributes. The
panelists placed the samples one by one into the mouth and
an additional sample was available upon request. For this
attribute group, all the panelists must chew the sample until it
becomes a homogeneous paste in the mouth in order to arrive
at the same evaluation point, independent of the speed and
strength of each panelist. A 15-min break was then held
before assessing the texture attribute group. For this group,
271
M. ELA
TABLE 2. LEXICON (TERMS AND DEFINITIONS) FOR SENSORY ANALYSES OF BREAD PRODUCTS DEVELOPED IN THIS STUDY
Sensory group
Appearance
Entire product
Descriptor
Definition
Reference*
Color
Shininess
Cracked
Intensity of color
Reflection of light on the piece
Presence of cracks on the surface of the sample
Flour
Number
Gap
Color
Color
Flour
Intensity of color
Intensity of color
Presence of flour on the low surface of the sample
Crust
Volume
Score
Cut product
Concavity
Convexity
Depth
Thickness
Crumb
Aroma
Taste
and flavor
Crust
Crumb
Crust
Color
Pore size
Pore regularity
Acetic acid
Butyric acid
Lactic acid
Butter
Lactic fat
Wood
Caramel
Smoke
Mouldy
Intensity of color
Size of the holes in the crumb
Homogeneity of the pores in the crumb
The sour aroma associated with vinegar
The aroma associated with regurgitated milk
The aroma associated with soured milk
The aroma associated with butter
The aroma associated with milk fat
The aroma associated with dry wood
The aroma associated with toasted sugar
The aroma associated with dust and fire
The aromatics associated with damp closed air
spaces
Pig fat
The aroma associated with animal fat
Those described for crust
Sweet
Sweet basic taste
Salty
Sour
Bitter
Pungent
Straw
Toasted
272
M. ELA
TABLE 2. CONTINUED
Sensory group
Texture
Crust
Descriptor
Definition
Yeasty
Oily
Aftertaste
Global intensity
Crumb
Those described for crust
Crustiness
Hardness
Elasticity
Friability
Graininess
Doughy
Chewiness
Mouth residue
Crumb
Reference*
M. ELA
FIG. 2. DISTANCES TO CONSIDER IN TESTING THE ATTRIBUTES OF CONCAVITY (a) AND CONVEXITY (b) ATTRIBUTES AND AN EXAMPLE OF A FLAT
BREAD (c)
particular attributes so that before assessing specific products, the attributes to evaluate should be established by consensus with the panel.
CONCLUSION
This study has proposed a methodology and a complete protocol for sensory analysis of bread and closely related products. The work conducted with the trained panel enabled an
objective understanding of bread and its characteristics. As a
consequence, a wide range of applications related to quality
evaluation and technological approaches can be conducted.
Such a standard protocol provides a better understanding of
the product, leads to management of its ingredients and the
process variables and brings the producer closer to the con-
TABLE 3. KEY FEATURES OF DEVELOPED METHODOLOGY FOR THE SENSORY EVALUATION OF BREAD
Attributes
Sample
General practices
4 samples (2 cm 2 cm)
15-min break
Texture
4 samples (2 cm 2 cm)
15-min break
Appearance
274
M. ELA
10
9.5
9
8.5
8
7.5
7
6.5
6
5.5
5
4.5
4
3.5
3
2.5
2
1.5
1
0.5
0
Brightness
Score width
Score depth
Score color
Convexity
Lactic fat
Salty
A B C D
FIG. 3. PLOT OF MEANS OF THE NINE ATTRIBUTES EVALUATED ON FOUR BREAD SAMPLES BY THE PANEL
Means followed by the same letter are not significantly different at P < 0.05 (Fishers least significant difference test). A (1.8% salt), B (0.9% salt), C
(1.8% salt, 5% butter), D (0% salt).
Brightness
Score width
Salty
Score depth
Lactic fat
Score color
Acetic acid
Crumb regularity
Convexity
sumer. Indeed, a consensus methodology for sensory evaluation of bread brings the possibility of improving quality
assessment, leading to the development of innovative products and combining all the information with consumers
data. Further training must be carried to optimize the panel.
However, this work could become an interesting innovation
tool for the bakery industry leading to standardized bread
sensory evaluation.
ACKNOWLEDGMENTS
The authors thank the financial support of Generalitat de
Catalunya from Generalitat de Catalunya (Spain) for the
training and formation of the panel. Also, they thank Dr. L.
Guerrero and M.D. Gurdia from Institut de Recerca I Tecnologia Agroalimntaries for their technical assessment. Likewise, we thank the group of judges for their dedication and
collaboration for the success of this panel.
TABLE 4. SUM OF SQUARES OF THE ANALYSIS OF VARIANCE OF NINE ATTRIBUTES EVALUATED ON FOUR BREAD SAMPLES BY THE PANEL
Source of variation
DF
Brightness
Score width
Score depth
Score color
Convexity
Crumb regularity
Acetic acid
Lactic fat
Salty
Product
Judge
Product judge
Residuals
Total
3
8
24
108
143
20.9*
163.1***
50.2
193.0
427.1
669.1***
42.3***
15.8
100.7
827.8
303.3***
134.9***
32.8
96.2
567.1
59.9***
83.8***
14.1
58.6
216.3
96.6***
107.0***
39.3
251.7
494.6
32.2***
40.1**
55.1
168.0
295.5
22.8**
224.5***
63.5
196.0
506.8
194.2***
105.6***
44.6
193.1
537.4
175.4***
70.9***
50.2
176.1
472.5
275
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