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Life
Universe = 13.8bya
Solar System = 4.6bya
Life = 3.8bya
1.8million species identified, thousands more each year, with 10100 million species in total, of which are arthopods
Characteristics of Life:
Reproduce
Grow and Develop
Metabolise
Respond to Stimuli/Environmental Changes
Have Cells (organizational units)
Possess the Chemicals of Life
o Carbohydrates
most abundant, chemically simple organic
molecules
store/transport energy (mostly in plants, animals
use lipids), structural components
monosaccharides link to form oligosaccharides (26) or polysaccharides
o Proteins
Dependent on amino acid sequence, linked by
peptide bonds
4 different levels of organisation (shapedependent)
o Lipids
fats, oils, waxes, cholesterol, fat-soluble vitamins
(A, D, E, K), monoglycerides, diglycerides,
phospholipids
energy storage, structural component of cell
membrane
o Nucleic Acids
formed by linking nucleotides
store/transfer genetic information
DNA, RNA
Prions (proteinaceous infectious particles) are altered proteins
that can change other proteins through conformation.
Domains (classification), defined by Carl Woese (compared
ribosomal RNA, formed phylogenetic tree):
Eukarya (35 subdivisions) - plantae, fungi, animalia, 50100 protist kingdoms
Bacteria (19 subdivisions)
Archaea (16 subdivisions) - many are extremophiles
(halophiles,
thermophiles,
methanogens
swamps/marshes, anaerobic and produce methane)
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Bacterial Morphology
and Colony Formation
Archaea
Cell membrane contains ether
linkages
Cell wall lacks peptidoglycan
Three RNS polymerases (like
eukaryotes - genes and enzymes
are more like this)
Archaea (and Eukarya) are not
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Macromole
cule
Subunit
Bond
Examples
Storage and Structure
Carbohydr
ate
Monosaccharid
es (glucose,
can form
disaccharides
like maltose)
Lipid
Fatty Acids
(In TAG, three
fatty acids
each join to a
glycerol by an
ester bond,
varying in
length, and
number and
positions of
double bonds)
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Glycosidic
Bond
Ester Bond
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Protein
Amino Acids
(20 different
ones that form
polypeptides
which fold into
3D structure)
Peptide
Bonds
Nucleic
Nucleotides
Phosphodies
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When polymerised they become a backbone with various sidechains that determine how it folds and 3D structure (primary,
secondary, tertiary and quaternary levels of folding determine final
shape)
Used as structure (keratin), storage
(casein), transport
(haemoglobin), hormones (insulin), movement (actin), enzymes
(sucrase)
Enzymes - catalytic proteins selectively speed up chemical reactions
without being consumed, allowing reactions to be fast enough for a
cell to survive
E (enzyme) + S (substrate) ES E + P
(product)
Catalysis occurs at the active site
Enzymes lower the activation energy (EA) of
a thermodynamically favourable reaction,
but do not affect the equilibrium or free
energy change (G - the difference in the
energy
between
the
reactants
and
products)
and
cannot
make
a
thermodynamically unfavourable reaction favourable
DNA or RNA, store hereditary information, polymers also called
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Acids
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ter Bonds
polynucleotides
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Cell Integrity
The membrane prevents unwanted nutrients and toxins from
entering/leaving, and hence maintains cell integrity. There were two
proposed models for the membrane:
Davson-Danielli Model (1935) - phospholipid bilayer with
proteins above and below
Fluid Mosaic Model (1972 by Singer and Nicholson) integral membrane proteins sat inside, peripheral
proteins above and below, with a cytoskeleton
supporting it; had sidedness or asymmetrical
distribution of proteins, carbohydrates and lipids (like
cholesterol) between each side as many were formed
inside the cell but cannot pass to the outside
o the fluidity refers to the rapid movement of lipids
and proteins laterally - shown by:
the fusing of mouse and human cells, and
proteins were mixed, not one-side human,
the other mouse
microscopy with staining
FRAP (fluorescence recovery after photobleaching) - altering DNA to produce
proteins that lose colour after laser beam
exposure to one section of the membrane,
and over time colour comes back as this
area is filled with non-zapped proteins
Membrane members:
Lipids - of which 0-25% is cholesterol; lipid rafts are
semi-solid molecules that keeps proteins together or
anchors them to the cytoskeleton
Proteins - both peripheral and integral that span the
membrane and shoot out either side but with different
domains on each side
Carbohydrates
(glycolipids
and
glycoproteins) - the addition of the
sugar groups allow cells to be
recognised by other proteins or present
different messages through a variety of
combinations
Sidedness is important for cell recognition and
adhesion
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Photosynthesis
The physico-chemical process is used by
plants, algae (oxygenic) and photosynthetic
bacteria (anoxygenic; uses bacterial chlorophylls) to
produce organic compounds with light as oxygen
(only 0.5% of the 21% is produced by NONbiological processes, the main sources being
cyanobacteria, plankton and plants), whilst
consuming the toxic CO2. Photosynthesis supplies all food, petrol
(and natural gas, coal and ethanol), and clothing and building
materials. In eukaryotes, photosynthesis occurs in chloroplasts
(green + form or entity), which are double membrane-bound
flat discs 2-10m in diameter and 1m thick, containing lots of small
discs called thylakoid (thylakos = sac) which consist of a thylakoid
membrane surrounding a thylakoid lumen, and exist as stacks called
grana (Latin for stacks of coins), connected by intergrana or
stroma thylakoids. This is placed in a thick fluid called the stroma
(the site of light-independent reactions). The pigment chlorophyll
is used to absorb light on the thylakoid membrane, and green light
is reflected whilst red and blue are mostly absorbed (by chlorophyll
a and b and carotenoids).
Photosynthesis occurs in two stages:
1. Light-Dependent Reactions - light captured, electron
and proton transfer reactions to make energy-carrying
molecules, produces ATP and NADPH
2. Light-Independent Reactions - ATP and NADPH used
to convert CO2 into glucose
ATP (adenosine-5-triphosphate) is produced by either redox
reactions or photons. If it is done by photons (sunlight) it is called
photophosphorylation (phosphorylation simply means adding
phosphate group). The light energy is converted into electrical
energy and packaged into chemical energy as ATP or NADPH
(nicotinamide adenine dinucleotide phosphate; considered energy
couriers - provide temporary storage of chemical energy). This
process is performed by photosystems (protein complexes that
contain chlorophyll) found in thylakoid membranes. The chlorophyll
are bound to proteins which act as antennae that absorb photons
and transfer the excited electron to the reaction centre.
First a photon hits a chlorophyll molecule surrounding the
Photosystem II (P680 as it absorbs a wavelength of 680mm penetrated faster than longer wavelengths, hence first), and the
chlorophyll molecules transmit energy from the excited elections in
the antenna complex to a reaction centre. Each photosystem has
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Ribulose-1,5-biphosphate carboxylase oxygen (RuBisCO) which catalyses carbon fixation to RuBP, probably most
abundant protein on Earth
Ribulose-1,5-biphosphate (RuBP) - 5-carbon sugar chain, CO 2
acceptor in first major step of carbon fixation
CO2 - used during fixation
ATP and NADPH - used in reduction phase to convert 3phosphoglycerate to glyceraldehyde-3-phosphate (three
carbon precursor to flucose), and ATP is used in regeneration
phase where it converts this back into RuBP
The first stage is carbon fixation, where RuBisCO attaches
CO2 to RuBP (6-carbons), which breaks into two phosphoglyceric
acids (3-PG as they have 3 carbons each). This is phosphorylated
(adds phosphate group) by ATP to form 1, 3-biphosphoglycerate,
then NADPH reduces this in the reduction phase into
glyceraldehyde-3-phosphate (G3P) - the ultimate goal of the Calvin
Cycle. This is composed of the simplest sugar known (D-aldotriose),
which can be combined to form organic molecules like fructose
(which can then be rearranged into glucose, or other molecules like
sucrose and starch). In the regeneration phase G3P can be
converted back to RuBP by ATP. In total, one glucose molecule
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(becomes part of
6)
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Note
that
arrows without lines within them only cover the exactly
length of the gene. Each cycle (production of new
copies) resulting in a doubling of molecules (2, 4, 8,
16220). As DNA polymerase is denatured at 90 oC,
the DNA polymerase from the thermophile Thermus
aquaticus (Taq) is used, which is stable at 98 oC, but
optimal at 70oC, allowing extending to be done at a higher
temperature than annealing (now 72oC - diagram shows for normal
DNA polymerase).
We now have automated PCR machines that can do 96
samples at once using solid states to rapidly increase and decrease
temperature (as common in a molecular lab as a photocopier is in
an office). PCR is incredibly sensitive and specific, targeting only
certain genes, and the electrophoresis can be applied (as DNA is
slightly negative moves to positive electrode, smaller molecules can
move through gel mesh more easily and hence move further, only
those replicated will be potent enough to see after staining with
fluorescent that glows in UV when bound to DNA).
Simple sequence repeats (SSR) are short base pair sequences
that repeat many times, with a different number for different people.
With around 120, 000 SSRs, and each being unique, it is easy to
identify a person by their DNA. PCR is used to amplify each specific
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SSR being analysed using primers designed for those SSRs and then
these bands are compared (only identical twins should have
identical patterns). Using this, we can identify people after disasters
(as in 9/11, comparing to kin), paternity testing (particularly with
celebrity heirs), deduce crime suspects (13 used by FBI), or prove
historical truths (Anastasia and the Romanovs). However, this
evidence can only be used for EXCLUSION, as you can prove that the
SSRs dont line up. If they do line up, inclusion cannot be proved as
this may be by happenstance. Mitchondria also have their own DNA
which comes entirely from the mother, which was used in cases like
identifying if Anastasia was still alive by comparing to another greatgrandchild of Queen Victoria. Hair cannot be used (as it is just
protein, no DNA), however hair follicles can be.
Mutation
A mutation is a change in the nucleotide sequence of an
organisms DNA, ultimately creating genetic diversity. They can also
occur in virus DNA or RNA. Mutations lead to diversity which is
critical to the survival of life. For example, the British Peppered Moth
had a mutation resulting in some light, some dark, which were
better at camouflage in either lichen-covered trees or soot-covered
industrial areas during the Industrial Revolution of the mid-19 th C
when pollution was being produced. They will only be inherited in
offspring if they occur in gametes.
Mutations can occur as:
point mutations (changes single base)
insertions
deletions
duplications of sequences
chromosomal rearrangements (like fusion, fission,
inversion and translocation)
Mutations can be caused by:
errors in DNA replication (DNA polymerase makes 1
error in 105 bases, leading to incorrect base-pairing,
however DNA repair enzymes reduce this to 1 in 1010)
mutagens
o chemicals (nicotine, asbestos, free radicals,
oxidising agents, nucleotide analogues) which
damage DNA
o radiation (natural radiation like uranium, nuclear
waste/bombs, medical X-rays, UV - 20, 000
pyrimidine dimers (e.g. T-T)/hour/cell are caused at
12pm in Sydneys Summer) which damages DNA
transposable DNA (jumping genes)
Damaged DNA (like the thymine dimers caused by UV
-adjacent thymines that bend towards each other through H-bonds which causes DNA to buckle due to their pull towards each other
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