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ticular proteins from rats receiving selenium. Selenium is also noted for its interaction with other trace
minerals,7 and we recently reported that cadmium
ameliorated the frequency of selenium-induced cataract.8 However, the lens contains a variety of other
trace minerals,9 yet it is not known if other trace elements modify selenium-induced cataracts. Most of
the selenium taken in by man is from the diet, yet
there is no evidence on whether or not oral administration of selenium is cataractogenic. Thus, the purposes of the experiments described below were: (1)
to compare 14 trace elements to selenium in their
abilities to promote cataract in the animal model,(2)
to determine if other trace elements ameliorate selenium-induced cataract, and (3) to determine if oral
administration of selenium promotes cataract formation.
Several laboratories have recently shown that injections of elevated quantities of the trace mineral
selenium to rats during the suckling period caused
the formation of bilateral nuclear cataracts.1"4 These
cataracts are interesting because of their speed of formation (3 days)4 and because they may be useful
models for cataracts caused by oxidant stressors. Selenium-induced cataracts have been characterized
histologically,' and several important biochemical
changes have been noted. For example, glutathione
and proteinaceous sulfhydryl levels were decreased,4
while the ratio of insoluble to soluble proteins and
H2O2 were increased in lenses of rats with seleniuminduced cataract5. Hess et al6 recently reported changes
in specific peptides in the soluble and insoluble lenFrom the Departments of Biochemistry and Ophthalmology,
The Oregon Health Sciences University, Portland, Oregon.
Presented in part at the Annual Meeting of the Association for
Research in Vision and Ophthalmology, Sarasota, Florida, May
1982.
Supported by NIH grant EY-3600 from the National Eye Institute and by the R. Blaine Bramble Medical Research Foundation.
Submitted for publication June 17, 1982.
Reprint requests: Thomas R. Shearer, PhD, Departments of
Biochemistry and Ophthalmology, The Oregon Health Sciences
University, Portland, OR 97201.
417
418
Element
Selenium
Tellurium
Arsenic
Molybdenum
Tungsten
Vanadium
Cyanide
Zinc
Iron
Lead
Cadmium
Copper
Silver
Chromium
Mercury
Ionic
form
Compound
injected
SeO 3 =
TeO-T
AsO 2 "
MoO 4 "
WO 4 "
VO3=
Na 2 SeO 3
K 2 TeO 3
NaAsO 2
Na 2 MoO 4
Na 2 WO 4
NH 4 VO 3
CN"
++
Zn
Fe + +
Pb + +
Cd + +
Cu + +
Ag+
Cr +3
Hg ++
Frequency
Dosage
of cataract
(fimoles
element/rat) (percent)
KCN
0.15
0.15
0.30
0.30
0.30
0.30
0.30
100
0
0
0
0
0
0
ZnCl 2
FeCl2
Pb(C 2 H 3 O 2 ) 2
CdCl2
Cu(C 2 H 3 O 2 ) 2
AgNO 3
CrCl3
HgCl2
0.76
0.45
0.30
0.30
0.30
0.30
0.30
0.15
0
0
0
0
0
0
0
0
Vol. 24
=
=
=
=
Not visible;
Visible;
Pronounced;
Fibers seemed separated.
No. 4
419
Fig. 1. Slit-lamp photographs of eyes of rats with selenium-induced cataract. A, Approximately 3 weeks after suckling rat pup
received subcutaneous injections of 0.15 umoles Se on days 5-9
postpartum. The central nucleus was opaque and the peripheral
nucleus showed diffuse opacity (asterisk). B, Nuclear cataract in
lens of animal receiving oral selenium, C, Eye of rat receiving
simultaneous injections of selenium and copper.
The most effective trace element studied for prevention of selenium-induced cataract was mercuric
ion (Table 3). At dosage levels equal to selenite, mer-
420
Vol. 24
0.0
0.11
0.23
0.46
96 2(10)*
0
91 2(10)
0
96 2 (9)
0
69 6 (7)
30
Frequency of cataract
Animals
Percent
0/10
0
0/10
0
10/10
100
7/7
100
Clear (10)
Clear(10)
Absent
Clear (10)
Clear (10)
Absent
Hypermature (6)
Hypermature (6)
Hypermature (6)
cury was totally effective in preventing selenium-induced cataract formation. Further titration of the
mercury dosage to half the molar level of selenium
(0.075 /imoles Hg per rat) still prevented cataract formation in 70% of the animals receiving selenium.
Zinc, iron, lead, chromium, molybdenum, tungstate, and vanadium had no influence on the frequency of selenium-induced cataract (Table 4). This
occurred despite the fact that the levels of these trace
minerals administered were two to five times the dosage level of selenium.
The data in Table 5 show the influence of protective trace minerals on the severity of selenium-induced cataract. Selenium alone caused very dense
cataract formation in the nucleus, resulting in an average nuclear score of 3.9. The peripheral nucleus was
only mildly involved, with an average score of 1.1.
Swollen fibers scored an average of 1.9 (pronounced).
The protective ions tellurium, copper, cadmium, arsenic, cyanide, and silver all tended to reduce the
severity of selenium-induced cataracts (Table 5).
Opacity scores in the central nucleus, peripheral nucleus, or swollen fibers tended to be lower in animals
receiving these ions along with selenium. The selenium plus mercury group could not be scored because
Trace mineral
Trace mineral
Frequency oj cataract
Freque,ncyof
cataract
Group
Dosage (\imoles
element/rat)
Form
Animals
Percent
Group
Dosage (^moles
element/rat)
Form
Animals
Percent
Se
Se + Te
Se + Cu
Se + Cd
Se + As
Se + CN
Se + Ag
Se + Hg
0.15
0.15
0.30
0.30
0.30
0.30
0.30
0.15
SeO 3 =
TeO 3 =
Cu + +
Cd + +
AsO 2 "
Cn"
Ag+
Hg + +
44/44
8/10
100
80
56
40
25
25
20
0
Se
Se + Zn
Se + Fe
Se + Pb
Se + Cr
Se + Mo
Se + W
Se + V
0.15
0.76
0.45
0.30
0.30
0.30
0.30
0.30
SeO 3 =
Zn + +
Fe + +
Pb + +
Cr +3
Mo<V
WO 4 "
Vo 3 -
44/44
11/11
10/10
10/10
10/10
10/10
10/10
100
100
100
100
100
100
100
100
5/9
4/10
2/8
2/8
2/10
0/10
9/9
No. 4
The studies presented above provided trace minerals in daily injections over a 5-day period. In another experiment, similar to previous studies,2"5 we
found that a single injection of 0.42 /umoles Se on
day 10 postpartum caused cataract formation in 90%
of the injected animals, and the average opacity score
for the central nucleus was 4.0 0 (9). Cataracts were
generally observable as soon as the eyes opened (14
days postpartum). A second group of animals receiving a single injection of 0.42 jumoles of Cd along with
the single injection of 0.42 ^moles Se showed a markedly decreased frequency (38%) and severity of cataract (central nucleus opacity score: 1.3 0.33).
421
Group
Se
Se
Se
Se
Se
Se
Se
Se
+ Te
+ Cu
+ Cd
+ As
+ CN
+ Ag
+ Hg
Central nucleus
Peripheral
nucleus
3.9
2.9
2.2
3.6
2.0
2.0
4.0
1.1
0.8
0.6
0.3
0.3
1.0
0.0
0.04 (29)*
0.4 (8)
0.6 (5)
0.4 (4)
1.0(2)
1.0(2)
0.0 (2)
0
0.1
0.3
0.2
0.1
0.3
1.0
0.0
Swollen
fibers
.9 0.1
.6 0.2
.4 0.7
.3 0.3
.0 1.0
().8 0.3
.5 0.5
0
Discussion
The data presented above emphasized two facets
of trace mineral-induced cataractogenesis: (1) compared to 14 other trace elements, selenium was a potent and extremely rapid promoter of cataracts; and
(2) the data also pointed out the rather dramatic influence trace element interactions can have on cataractogenesis.
Regarding the comparative strength of selenium
in promoting cataracts, we attempted to inject as
much of the other trace minerals so that dosage levels
were equimolar or greater than selenium, yet so as
not to exceed moderate toxicity levels of the elements.
Mercury and tellurium could be reasonably tolerated
only at levels equimolar to selenium. The animals in
the groups receiving zinc, iron, lead, silver, copper,
cyanide, arsenic, cadmium, chromium, molybdenum, tungsten, and vanadium received from two to
five times the dosage level of selenium. Despite these
higher dosages, none of the animals receiving these
elements alone showed any form of significant cataract at 27 days postpartum. On the other hand, 100%
of the animals receiving selenium developed very
dense bilateral nuclear cataracts that were visible as
soon as the eyes opened. It is possible that the other
elements could have produced cataracts under more
long-term exposure or after a longer latent period.
However, the data serve to emphasize how potent
selenium is in promoting cataract in a short period
of time.
Other investigators have reported that a single injection of selenium on day 10 postpartum will promote nuclear cataract formation in the rat, and that
the rabbit and guinea pig are also susceptible to this
cataract.2"4 Our present slit-lamp study revealed that
several other lenticular changes can be present along
with the obvious central nuclear opacity. A less dense
nuclear opacity was observed surrounding the central
nuclear cataract, and a single layer of a spoke-like
pattern of swollen lenticular fibers extended around
the peripheral nuclear opacity to the Y suture. Transmission electron microscopy of lens fibers from selenium-induced cataracts showed no changes in the
apposition of one fiber to another, but rather extensive vacuolization of the cytoplasm within the fibers.10 Although the data presented in this report did
not show an increase in overall wet weight of selenium treated lenses (Table 6), other studies5 using
higher dosages of selenium showed a significant increase in the water content of hypermature cataracts.
Extensive vacuolization of the lens cortex was also
noted in a previous study where high doses of selenium were repeatedly injected over many days.1
These data may indicate that selenium cataract interferes with the normal functioning of the cation
pump, but this is speculation at the present time.
Another interesting feature of selenium-induced
cataracts is the fact that nuclear cataracts appear
within 3 to 4 days after selenium injection.4 Obviously, cells undergoing differentiation and appositional growth at the lens bow at the time of selenium
injection would not have sufficient time to migrate
Table 6. Selenium concentrations and wet weights
of lenses after multiple injections of protective
trace elements with selenite
Lens
Group
Control
Se
Se + Te
Se + Cu
Se + Cd
Se + As
Se + CN
Se +Ag
Se + Hg
1 (3)*
1 (3)
1 (3)
2(3)
0(3)
1(3)
0(3)
1(3)
K3)
Selenium (ppm)
0.53
0.63
0.78
0.65
0.56
0.66
0.57
0.57
0.56
0.03
0.04
0.15
0.15
0.16
0.09
0.11
0.06
0.06
(3)
(3)
(3)
(3)
(3)
(3)
(3)
(2)
(3)
422
into the nuclear region and form a cataract. Furthermore, studies using cataractous doses of selenium
tagged with 75Se indicate that there is very little 75Se
actually in the nuclear cataract.* These data indicate
that the site of action of selenium is extrinsic to the
nuclear cataract itself.
It should be noted that the ability of selenium to
induce cataract is dependent on the age of the animal.
After 18 days postpartum, selenium injections no longer cause permanent cataracts in rats.2 Why the sensitivity of the lens to selenium changes with time is
unknown, but investigations into this subject would
be useful since they may help pinpoint the biochemical basis for selenium-induced cataract.
Although the data in Table 6 showed no statistically significant increases in the selenium content of
lenses of animals receiving selenium injection, these
data should not be interpreted as showing that the
lens does not take up some selenium. Besides the
analytical variability in measuring small levels of selenium in the lens, the data may be misinterpreted
because selenium was measured almost 3 weeks after
the last selenium injection. Two other reports419 indicated that after a single injection of selenium, lens
selenium levels peak 6-24 hr postinjection, and
75
Se levels fall rapidly in the lens thereafter.* The
critical time period for cataract formation from selenium is probably within the first 3-4 days after injection. Future studies could test if some of the protective ions influence lens selenium levels at these
early time periods.
The biochemical mechanism of action of selenium
in cataract promotion may be related to the affinity
of selenite for sulfhydryl groups. Selenium in other
soft and hard tissues of the body is associated with
proteins, as selenoamino acids (ie, selenocysteine)
and as selenotrisulfides (R-S-Se-S-R).'' Selenotrisulfides are reduced further to selenopersulfides (R-SeH) by glutathione reductase and by excess reduced
glutathione, as is found in the lens. Selenite causes
the nonenzymatic oxidation of sulfhydryl groups to
form disulfides and selenotrisulfides.4'11 Two reports
described reduced levels of sulfhydryl groups in selenium-induced cataracts.4'5 It has been proposed that
selenite is cataractogenic by causing the formation of
cataractous disulfide linkages in lens proteins.5 However, we recently found that total lens disulfides were
not increased in selenite cataract. This does not exclude the possibility of disulfide formation in certain
classes of proteins or at specific anatomical sites.
The survey of 14 trace minerals in the present study
revealed six elements that offered significant protec-
Vol. 24
No. 4
7.
8.
9.
10.
11.
12.
13.
Acknowledgments
The authors wish to express appreciation to Dr. Earl
Palmer and Mr. Pat Wallace for help with the slit-lamp
biomicroscopy, and to Dr. Jack Fellman for helpful discussions.
14.
15.
References
16.
1. Shearer TR, McCormack DW, DeSart DJ, Britton JL, and
Lopez MT: Histological evaluation of selenium induced cataracts. Exp Eye Res 31:327, 1980.
2. Ostadalova I, Babicky A, and Obenberger J: Cataractogenic
and lethal effect of selenite in rats during postnatal ontogenesis.
Physiol Bohemoslov 28:393, 1979.
3. Bhuyan KC, Bhuyan DK, and Podos SM: Selenium-induced
cataract and its possible biochemical mechanism. In Selenium
in Biology and Medicine, Second International Symposium,
Spallholz JE, editor. Westport Conn. AVI Publishing, 1981.
4. Bunce GE and Hess JL: Biochemical changes associated with
selenite-induced cataract in the rat. Exp Eye Res 33:505, 1981.
5. Bhuyan KC, Bhuyan DK, and Podos SM: Cataract induced
by selenium in the rat: I. Effect on the lenticular protein and
thiols. II. Increased lipid peroxidation and impairment of enzymatic defense against oxidative damage. IRCS Med Sci: The
Eye 9:194; 195, 1981.
6. Hess JL, Wisthoff F, and Schwab SJ: Effect of selenium on
17.
18.
19.
20.
21.
423