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PS-2820
Part Number
PS-2002
PS-2110
PS-2500
SE-7288
Purpose
The purpose of the activity is to compare the production of carbon dioxide gas by germinating
seeds with the production of carbon dioxide gas by dormant seeds. Also, determine the effect of
temperature on seed germination.
Background
Dormant seeds contain all the materials required to start new plant growth including starches,
enzymes, DNA to guide the process, RNA, and all the necessary cellular machinery. They need
water and oxygen to start the process called germination, which is the process of initiating new
plant growth from a seed.
When seeds become moist, the water is first absorbed by the seeds covering, which softens and
allows water to penetrate the seed. As soon as the cells inside the seed absorb the water, the
enzymes, which must be suspended in water to function, begin the process of cellular respiration
to provide the new plant with energetic adenosine triphosphate (ATP) molecules that it needs to
fuel the building of new plant material.
The process of cellular respiration converts sugar and oxygen and other substances into ATP,
water, and carbon dioxide gas. Therefore, carbon dioxide gas production is evidence of cellular
respiration in germinating seeds.
Pre-lab Questions
Compare dormant seeds and germinating seeds.
1.
2.
How will the amount of carbon dioxide gas given off by dormant seeds compare to the
amount of carbon dioxide gas given off by germinating seeds?
2005 PASCO
p. 9
Activity 2
PS-2820
3.
How will the amount of gas given off by cold germinating seeds compare to the amount of
gas given off by warm (room temperature) seeds?
4.
What other conditions beside temperature might affect the rate of production of gas by
germinating seeds?
Safety Precautions
Procedure
GLX Setup
1.
2.
Equipment Setup
3.
4.
Put the end of the CO2 Gas Sensor into the sampling bottle. Firmly plug
the end of the sampling bottle with the rubber stopper.
Note: Avoid bumping the equipment because jarring or bumping the CO2
Gas Sensor may cause it to record erratically.
on the GLX.
6.
7.
Remove the seeds from the sampling bottle and examine them closely.
Write a description of how they look in the Lab Report section.
8.
Put 20 germinating seeds into the sampling bottle. Put the CO2 Gas
Sensor into the bottle so that the rubber stopper firmly plugs the end of the bottle.
9.
10.
on the GLX.
2005 PASCO
p. 10
Activity 2
11.
PS-2820
Fill a large beaker about half full of water and add about half a liter of cubed or crushed
ice.
13.
Return the CO2 Gas Sensor to the sampling bottle so that the rubber stopper firmly plugs
the end of the bottle.
14.
Put the sampling bottle into the ice water in the beaker and hold the neck of the sampling
bottle so that most of the bottle remains in the ice water.
15.
16.
17.
Remove the sampling bottle from the ice water and dry the outside of the bottle.
18.
Remove the seeds from the sampling bottle and examine them closely. Write a description
of how the germinating seeds look in the Lab Report section.
19.
Follow instructions for disposing of materials and putting away the equipment.
on the GLX.
Analysis
1.
Draw a sketch of your CO2 concentration versus time graph as requested in the Lab Report
section.
2.
Use your recorded data to find the change in CO2 concentration for the dormant seeds, the
germinating seeds, and the germinating seeds at a low temperature.
3.
In the Graph Screen, press F3 to open the Tools menu. Select Statistics and press the
Activate key. The Statistics show the minimum and maximum values.
4.
Calculate the rate of change of CO2 concentration versus time, or the ratio of CO2
concentration (in ppm) divided by the time (in minutes), for each run of data.
How do your results compare with others in your class?
2005 PASCO
p. 11
Activity 2
PS-2820
Appendix:
To open a specific GLX file, go to the Home Screen (press
). In the Home Screen, select Data
Files and press the Activate (
) key. Use the cursor keys to navigate to the file you want. Press
F1 (
) to open the file.
2005 PASCO
p. 12
Activity 2
PS-2820
2.
How will the amount of carbon dioxide gas given off by dormant seeds compare to the
amount of carbon dioxide gas given off by germinating seeds?
3.
How will the amount of gas given off by cold germinating seeds compare to the amount of
gas given off by warm (room temperature) seeds?
4.
What other conditions beside temperature might affect the rate of production of gas by
germinating seeds?
Data
Description of Seeds
Dry, dormant seeds
Germinating seeds
2005 PASCO
p. 13
Activity 2
PS-2820
Data
Make a sketch of one run of CO2 concentration versus time, including labels for the y- and xaxes.
Data Table
Run
Initial CO2
concentration
Final CO2
concentration
Total time
Rate of CO2
production
Questions
1.
How does the rate of CO2 production for germinating seeds compare to the rate of CO2
production for the dry, dormant seeds?
2.
How does the rate of CO2 production for cold germinating seeds compare to the rate of
CO2 production for the room temperature germinating seeds?
3.
How does the appearance of the germinating seeds compare to the appearance of the dry,
dormant seeds?
2005 PASCO
p. 14