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3

Allozyme
CODE
D0101
D0102
D0103
D0104
D0105
D0106
D0107
D0108
D0109
D0110
D0111
D0112
D0113
D0114
D0115
D0116
D0117
D0118
D0119
D0120
D0121
D0122
D0123
D0124
D0125
D0126
D0127
D0128
D0129
D0130
D0131
D0132
D0133
D0134
D0135
D0136
D0137
D0138
D0139
D0140
D0141
D0142
D0143
D0144
D0145

120

30
RVA

SITE
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVA
RVB
RVB
RVB
RVB
RVB
RVB
RVB
RVB
RVB
RVB
RVB
RVB
RVB
RVB
RVB

EST
100
80
90
98
100
82
94
100
88
90
100
90
80
84
100
100
100
94
94
100
100
92
94
90
92
100
100
100
100
96
90
80
92
100
100
100
100
100
94
96
98
100
80
92
90

RVB
FBA
98
86
92
100
98
100
98
100
90
80
84
100
100
100
94
94
100
92
94
90
92
100
100
100
100
96
90
80
92
100
100
100
96
90
80
92
100
100
100
100
100
100
90
98
82

30

30
LKA

100
90
80
84
100
100
100
94
94
100
90
80
84
100
100
100
84
98
86
92
100
100
100
98
94
84
100
100
100
92
100
100
100
100
100
84
94
100
100
92
94
90
92
100
100

LKB
MDH
90
100
90
80
84
100
100
100
86
92
100
100
100
98
94
84
100
100
92
100
100
100
100
100
94
100
100
92
94
90
92
100
100
100
100
96
90
80
92
100
100
100
90
90
98

30

100
86
92
98
86
92
100
100
100
98
94
84
100
100
100
90
100
90
80
84
100
100
100
92
100
100
100
100
100
100
100
92
94
90
92
100
100
100
100
96
90
80
92
100
100

96
86
98
80
100
100
90
98
82
100
100
96
86
98
80
100
100
90
92
98
86
92
100
92
100
100
100
100
100
100
100
92
94
90
92
100
100
100
100
96
90
80
92
100
100

D0146
D0147
D0148
D0149
D0150
D0151
D0152
D0153
D0154
D0155
D0156
D0157
D0158
D0159
D0160
D0161
D0162
D0163
D0164
D0165
D0166
D0167
D0168
D0169
D0170
D0171
D0172
D0173
D0198
D0175
D0176
D0177
D0178
D0179
D0180
D0196
D0182
D0194
D0190
D0196
D0186
D0192
D0188
D0198
D0190
D01100
D0192
D0180

RVB
RVB
RVB
RVB
RVB
RVB
RVB
RVB
RVB
RVB
RVB
RVB
RVB
RVB
RVB
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKA
LKB
LKB
LKB

96
86
98
80
100
100
90
98
82
90
98
100
100
100
86
90
94
100
100
100
82
90
86
100
100
90
92
98
86
92
100
100
100
98
94
84
100
100
100
100
100
90
98
100
100
90
94
100

90
98
100
98
96
86
98
80
100
100
90
90
96
86
98
80
100
100
90
98
82
94
100
100
100
82
86
98
80
100
90
80
84
100
100
100
94
94
92
98
86
92
100
98
94
90
100
90

100
100
96
90
80
92
100
100
90
98
82
90
98
100
86
90
94
100
100
100
82
100
90
98
84
100
100
90
92
98
86
92
100
96
86
98
80
100
86
98
80
100
100
90
92
92
100
100

82
90
98
96
86
98
80
100
100
90
100
90
80
84
100
100
100
94
94
82
98
100
100
100
86
90
94
100
100
100
82
100
90
92
98
86
92
100
90
96
86
98
80
100
100
100
98
100

96
86
98
80
100
100
90
98
90
98
100
100
100
100
100
90
96
86
98
80
100
100
90
98
82
100
100
96
86
98
80
100
100
90
92
98
86
92
100
100
90
92
98
86
92
90
80
84

96
86
100
86
92
98
86
92
100
100
100
98
94
84
100
100
100
90
100
90
80
84
100
100
100
92
100
100
100
100
100
100
100
92
94
90
92
100
100
100
100
96
90
80
92
90
92
98

D0194
D0190
D0196
D0184
D0198
D0186
D0200
D0201
D0202
D0203
D0204
D0205
D0206
D0207
D0208
D0209
D0210
D0211
D0212
D0213
D0214
D0215
D0216
D0217
D0218
D0219
D0220

LKB
LKB
LKB
LKB
LKB
LKB
LKB
LKB
LKB
LKB
LKB
LKB
LKB
LKB
LKB
LKB
LKB
LKB
LKB
LKB
LKB
LKB
LKB
LKB
LKB
LKB
LKB

100
100
82
100
90
98
84
100
100
90
92
98
86
92
100
96
86
98
80
100
86
98
80
100
100
90
92

80
84
100
100
100
92
100
100
100
100
100
100
100
92
94
90
92
100
100
100
100
96
90
80
92
100
100

100
98
94
84
100
100
92
100
100
100
100
100
94
100
100
92
94
90
92
100
100
100
100
96
90
80
92

90
80
84
100
100
100
94
94
100
92
94
90
92
100
100
100
100
96
90
80
92
100
100
100
96
90
80

100
100
100
94
94
100
100
92
94
90
92
100
100
100
100
96
90
80
92
100
100
100
100
100
94
96
98

86
92
100
100
100
98
94
84
100
100
100
100
100
90
98
100
100
90
94
100
100
100
82
100
90
98
84

Allele Frequencies by Population for Codominant Data


*With Step by Step option
Data Sheet
Sheet1
Data Title
Allozyme
No. Loci
No. Samples
No. Pops.

3
120
4

Allele Counts and Total Count by Population


Locus
EST

Locus2

Locus3

Allele/n RVA
N
80
82
84
86
88
90
92
94
96
98
100
N
80
82
84
86
90
92
94
96
98
100
N
80
82
84
86
90
92
94
96
98

RVB
60
4
1
2
1
1
7
6
7
2
4
25
60
3
0
6
2
5
5
6
0
3
30
60
3
1
2
5
4
6
1
2
6

LKA
60
5
2
0
4
0
9
3
1
5
9
22
60
4
2
2
2
10
5
2
3
5
25
60
3
0
1
4
6
8
3
4
5

LKB
60
3
3
2
4
0
6
4
6
0
7
25
60
3
3
1
6
7
5
4
2
7
22
60
4
1
1
4
8
8
1
3
5

60
4
1
2
3
0
8
7
2
2
4
27
60
4
0
2
0
6
8
6
3
2
29
60
2
1
3
1
7
5
6
2
5

100

30

26

25

28

Allele Frequencies and Sample Size by Populations


Locus
EST

Locus2

Locus3

Allele/n RVA
RVB
LKA
LKB
N
30
30
30
30
80
0.067
0.083
0.050
0.067
82
0.017
0.033
0.050
0.017
84
0.033
0.000
0.033
0.033
86
0.017
0.067
0.067
0.050
88
0.017
0.000
0.000
0.000
90
0.117
0.150
0.100
0.133
92
0.100
0.050
0.067
0.117
94
0.117
0.017
0.100
0.033
96
0.033
0.083
0.000
0.033
98
0.067
0.150
0.117
0.067
100
0.417
0.367
0.417
0.450
N
30
30
30
30
80
0.050
0.067
0.050
0.067
82
0.000
0.033
0.050
0.000
84
0.100
0.033
0.017
0.033
86
0.033
0.033
0.100
0.000
90
0.083
0.167
0.117
0.100
92
0.083
0.083
0.083
0.133
94
0.100
0.033
0.067
0.100
96
0.000
0.050
0.033
0.050
98
0.050
0.083
0.117
0.033
100
0.500
0.417
0.367
0.483
N
30
30
30
30
80
0.050
0.050
0.067
0.033
82
0.017
0.000
0.017
0.017
84
0.033
0.017
0.017
0.050
86
0.083
0.067
0.067
0.017
90
0.067
0.100
0.133
0.117
92
0.100
0.133
0.133
0.083
94
0.017
0.050
0.017
0.100
96
0.033
0.067
0.050
0.033
98
0.100
0.083
0.083
0.083
100
0.500
0.433
0.417
0.467

Allele Frequencies by Population with Graph over Loci for Codominant Data
Data Sheet
Data Title

Sheet1
Allozyme

No. Loci
No. Samples
No. Pops.

3
120
4

Allele Frequency

EST

Locus2

Allele Frequencies by Populations

Locus2

Allele

RVA
80
82
84
86
88
90
92
94
96
98
100
80
82
84
86
90
92
94

RVB
0.067
0.017
0.033
0.017
0.017
0.117
0.100
0.117
0.033
0.067
0.417
0.050
0.000
0.100
0.033
0.083
0.083
0.100

LKA
0.083
0.033
0.000
0.067
0.000
0.150
0.050
0.017
0.083
0.150
0.367
0.067
0.033
0.033
0.033
0.167
0.083
0.033

LKB
0.050
0.050
0.033
0.067
0.000
0.100
0.067
0.100
0.000
0.117
0.417
0.050
0.050
0.017
0.100
0.117
0.083
0.067

0.067
0.017
0.033
0.050
0.000
0.133
0.117
0.033
0.033
0.067
0.450
0.067
0.000
0.033
0.000
0.100
0.133
0.100

98

94

90

84

Locus3

Locus

Locus
EST

80

98

94

90

84

80

98

94

90

86

82

Frequency

0.600
0.400
0.200
0.000

Locus3

96
98
100
80
82
84
86
90
92
94
96
98
100

0.000
0.050
0.500
0.050
0.017
0.033
0.083
0.067
0.100
0.017
0.033
0.100
0.500

0.050
0.083
0.417
0.050
0.000
0.017
0.067
0.100
0.133
0.050
0.067
0.083
0.433

0.033
0.117
0.367
0.067
0.017
0.017
0.067
0.133
0.133
0.017
0.050
0.083
0.417

0.050
0.033
0.483
0.033
0.017
0.050
0.017
0.117
0.083
0.100
0.033
0.083
0.467

RVA

Locus3

98

94

90

84

80

RVB
LKA
LKB

Allele Frequencies with Graphs by Population and Locus for Codominant Data
Data Sheet
Data Title

Sheet1
Allozyme

No. Loci
No. Samples
No. Pops.

3
120
4

Allele Frequency for EST


0.600
0.400
Frequency

0.200
0.000
80

82

84

86

88

90
EST

92

94

96

98

100

Locus

Allele Frequency for Locus2


0.600
0.400
Frequency

0.200
0.000
80

82

84

86

90
92
Locus2

94

96

98

100

94

96

98

100

Locus

Allele Frequency for Locus3


0.600
0.400
Frequency

0.200
0.000
80

82

84

86

90
92
Locus3
Locus

Allele Frequencies by Populations For Graph By Locus


Locus
EST

Allele

Locus
Locus2

Allele

Locus
Locus3

Allele

RVA
80
82
84
86
88
90
92
94
96
98
100

RVB
0.067
0.017
0.033
0.017
0.017
0.117
0.100
0.117
0.033
0.067
0.417

RVA
80
82
84
86
90
92
94
96
98
100

RVB
0.050
0.000
0.100
0.033
0.083
0.083
0.100
0.000
0.050
0.500

RVA
80
82
84
86
90
92
94
96
98
100

LKA
0.083
0.033
0.000
0.067
0.000
0.150
0.050
0.017
0.083
0.150
0.367
LKA
0.067
0.033
0.033
0.033
0.167
0.083
0.033
0.050
0.083
0.417

RVB
0.050
0.017
0.033
0.083
0.067
0.100
0.017
0.033
0.100
0.500

LKB
0.050
0.050
0.033
0.067
0.000
0.100
0.067
0.100
0.000
0.117
0.417
LKB
0.050
0.050
0.017
0.100
0.117
0.083
0.067
0.033
0.117
0.367

LKA
0.050
0.000
0.017
0.067
0.100
0.133
0.050
0.067
0.083
0.433

0.067
0.017
0.033
0.050
0.000
0.133
0.117
0.033
0.033
0.067
0.450

0.067
0.000
0.033
0.000
0.100
0.133
0.100
0.050
0.033
0.483
LKB

0.067
0.017
0.017
0.067
0.133
0.133
0.017
0.050
0.083
0.417

0.033
0.017
0.050
0.017
0.117
0.083
0.100
0.033
0.083
0.467

nant Data

RVA
RVB
LKA
94

96

98

100

LKB

RVA
RVB
LKA
96

98

100

LKB

RVA
RVB
LKA
96

98

100

LKB

Allele Frequencies at EST with Graphs by Population for Codominant Data


Data Sheet
Data Title
No. Loci
No. Samples
No. Pops.

Sheet1
Allozyme
3
120
4

Allele Frequency at EST for RVA (n=30)

84; 3%
86; 2%
100; 42%
88; 2%
90; 12%
92; 10%
98; 7% 96; 3% 94; 12%
80; 7%

82; 2%

Allele Frequency at EST for RVB (n=30)

80; 8% 82; 3%
86; 7%
100; 37%
90; 15%
92; 5%
98; 15% 96; 8%

94; 2%

Allele Frequency at EST for LKA (n=30)

80; 5% 82; 5%
100; 42%

98; 12%

84; 3%
86; 7%
90; 10%
92; 7%

94; 10%

Allele Frequency at EST for LKB (n=30)

80; 7%

82; 2%

84; 3%

Allele Frequency at EST for LKB (n=30)

80; 7%

82; 2%

84; 3%
86; 5%
100; 45%
90; 13%
92; 12%
98; 7% 96; 3% 94; 3%

Allele Frequencies by Population for EST


Allele

RVA
80
82
84
86
88
90
92
94
96
98
100

Allele

0.067
0.017
0.033
0.017
0.017
0.117
0.100
0.117
0.033
0.067
0.417
RVB

80
82
84
86
88
90
92
94

0.083
0.033
0.000
0.067
0.000
0.150
0.050
0.017

96
98
100
Allele

0.083
0.150
0.367
LKA

80
82
84
86
88
90
92
94
96
98
100
Allele

0.050
0.050
0.033
0.067
0.000
0.100
0.067
0.100
0.000
0.117
0.417
LKB

80
82
84
86
88
90
92
94
96
98
100

0.067
0.017
0.033
0.050
0.000
0.133
0.117
0.033
0.033
0.067
0.450

=30)

=30)

=30)

=30)

=30)

Allele Frequencies at Locus2 with Graphs by Population for Codominant Data


Data Sheet
Data Title
No. Loci
No. Samples
No. Pops.

Sheet1
Allozyme
3
120
4

Allele Frequency at Locus2 for RVA (n=30)

80; 5%
100; 50%
98; 5%

84; 10%
86; 3%
90; 8%
92; 8%
94; 10%

Allele Frequency at Locus2 for RVB (n=30)

82; 3%
84; 3%
86; 3%
100; 42%
90; 17%
92; 8%
96; 5% 94; 3%
80; 7%

98; 8%

Allele Frequency at Locus2 for LKA (n=30)

80; 5% 82; 5%
100; 37%

98; 12% 96; 3% 94; 7%

84; 2%
86; 10%
90; 12%
92; 8%

Allele Frequency at Locus2 for LKB (n=30)

80; 7%

84; 3%

Allele Frequency at Locus2 for LKB (n=30)

80; 7%
100; 48%
98; 3%

Allele Frequencies by Population for Locus2


Allele

RVA
80
82
84
86
90
92
94
96
98
100

Allele

0.050
0.000
0.100
0.033
0.083
0.083
0.100
0.000
0.050
0.500
RVB

80
82
84
86
90
92
94
96
98

0.067
0.033
0.033
0.033
0.167
0.083
0.033
0.050
0.083

84; 3%
90; 10%
92; 13%
94; 10%
96; 5%

100
Allele

0.417
LKA

80
82
84
86
90
92
94
96
98
100
Allele

0.050
0.050
0.017
0.100
0.117
0.083
0.067
0.033
0.117
0.367
LKB

80
82
84
86
90
92
94
96
98
100

0.067
0.000
0.033
0.000
0.100
0.133
0.100
0.050
0.033
0.483

nant Data

n=30)

n=30)

n=30)

n=30)

n=30)

Allele Frequencies at Locus3 with Graphs by Population for Codominant Data


Data Sheet
Data Title
No. Loci
No. Samples
No. Pops.

Sheet1
Allozyme
3
120
4

Allele Frequency at Locus3 for RVA (n=30)

80; 5%

82; 2% 84; 3%
86; 8%
90; 7%
100; 50%
92; 10%
94; 2%
98; 10%
96; 3%

Allele Frequency at Locus3 for RVB (n=30)

80; 5% 84; 2%
100; 43%

98; 8% 96; 7%

86; 7%
90; 10%
92; 13%
94; 5%

Allele Frequency at Locus3 for LKA (n=30)

80; 7%

82; 2%

84; 2%

100; 42%

86; 7%
90; 13%
92; 13%
98; 8% 96; 5% 94; 2%

Allele Frequency at Locus3 for LKB (n=30)

80; 3% 82; 2% 84; 5%

Allele Frequency at Locus3 for LKB (n=30)

80; 3% 82; 2% 84; 5%


86; 2%
100; 47%
90; 12%
92; 8%
94; 10%
98; 8% 96; 3%

Allele Frequencies by Population for Locus3


Allele

RVA
80
82
84
86
90
92
94
96
98
100

Allele

0.050
0.017
0.033
0.083
0.067
0.100
0.017
0.033
0.100
0.500
RVB

80
82
84
86
90
92
94
96
98

0.050
0.000
0.017
0.067
0.100
0.133
0.050
0.067
0.083

100
Allele

0.433
LKA

80
82
84
86
90
92
94
96
98
100
Allele

0.067
0.017
0.017
0.067
0.133
0.133
0.017
0.050
0.083
0.417
LKB

80
82
84
86
90
92
94
96
98
100

0.033
0.017
0.050
0.017
0.117
0.083
0.100
0.033
0.083
0.467

nant Data

n=30)

n=30)

n=30)

n=30)

n=30)

Allele Frequencies by Locus for Codominant Data


*With Step by Step option
Data Sheet
Sheet1
Data Title
Allozyme
No. Loci
No. Samples
No. Pops.

3
120
4

Allele Frequencies, Sample Size and Counts by Locus


Pop
RVA

RVB

LKA

Allele/n EST
N
80
82
84
86
88
90
92
94
96
98
100
N
80
82
84
86
88
90
92
94
96
98
100
N
80
82
84
86
88
90
92
94

Count
30
0.067
0.017
0.033
0.017
0.017
0.117
0.100
0.117
0.033
0.067
0.417
30
0.083
0.033
0.000
0.067
0.000
0.150
0.050
0.017
0.083
0.150
0.367
30
0.050
0.050
0.033
0.067
0.000
0.100
0.067
0.100

Locus2
60
4
1
2
1
1
7
6
7
2
4
25
60
5
2
0
4
0
9
3
1
5
9
22
60
3
3
2
4
0
6
4
6

30
0.050
0.000
0.100
0.033
0.083
0.083
0.100
0.000
0.050
0.500
30
0.067
0.033
0.033
0.033
0.167
0.083
0.033
0.050
0.083
0.417
30
0.050
0.050
0.017
0.100
0.117
0.083
0.067

Count

Locus3
60
3
0
6
2
0
5
5
6
0
3
30
60
4
2
2
2
0
10
5
2
3
5
25
60
3
3
1
6
0
7
5
4

30
0.050
0.017
0.033
0.083
0.067
0.100
0.017
0.033
0.100
0.500
30
0.050
0.000
0.017
0.067
0.100
0.133
0.050
0.067
0.083
0.433
30
0.067
0.017
0.017
0.067
0.133
0.133
0.017

96
98
100
LKB

N
80
82
84
86
88
90
92
94
96
98
100

0.000
0.117
0.417
30
0.067
0.017
0.033
0.050
0.000
0.133
0.117
0.033
0.033
0.067
0.450

0
7
25
60
4
1
2
3
0
8
7
2
2
4
27

0.033
0.117
0.367
30
0.067
0.000
0.033
0.000
0.100
0.133
0.100
0.050
0.033
0.483

2
7
22
60
4
0
2
0
0
6
8
6
3
2
29

0.050
0.083
0.417
30
0.033
0.017
0.050
0.017
0.117
0.083
0.100
0.033
0.083
0.467

Count
60
3
1
2
5
0
4
6
1
2
6
30
60
3
0
1
4
0
6
8
3
4
5
26
60
4
1
1
4
0
8
8
1

3
5
25
60
2
1
3
1
0
7
5
6
2
5
28

Heterozygosity, Fstatistics and Polymorphism by Population for Codominant Data


*With Step by Step option
Data Sheet
Sheet1
Data Title
Allozyme
No. Loci
No. Samples
No. Pops.

3
120
4

Sample Size, No. Alleles, No. Effective Alleles, Information Index, Observed Heterozygosity
Pop
RVA

RVB

LKA

LKB

Locus
EST
Locus2
Locus3
EST
Locus2
Locus3
EST
Locus2
Locus3
EST
Locus2
Locus3

Na
30
30
30
30
30
30
30
30
30
30
30
30

Ne
11.000
8.000
10.000
9.000
10.000
9.000
9.000
10.000
10.000
10.000
8.000
10.000

I
4.489
3.448
3.488
4.959
4.412
4.225
4.500
5.279
4.380
4.018
3.564
3.838

Ho
1.889
1.634
1.708
1.863
1.861
1.797
1.850
1.968
1.825
1.798
1.638
1.764

0.900
0.767
0.633
0.900
0.800
0.367
0.833
0.933
0.800
0.800
0.733
0.733

Mean and SE over Loci for each Pop


Pop
RVA

Na

Ne

Ho

Mean
SE

30.000
0.000

9.667
0.882

3.808
0.340

1.744
0.076

0.767
0.077

RVB

Mean
SE

30.000
0.000

9.333
0.333

4.532
0.220

1.841
0.022

0.689
0.164

LKA

Mean
SE

30.000
0.000

9.667
0.333

4.719
0.282

1.881
0.044

0.856
0.040

LKB

Mean
SE

30.000
0.000

9.333
0.667

3.807
0.132

1.733
0.049

0.756
0.022

Grand Mean and SE over Loci and Pops


N
Total

Mean
SE

Na
30.000
0.000

Ne
9.500
0.261

I
4.217
0.166

Ho
1.800
0.029

0.767
0.044

F-Statistics and Estimates of Nm over All Pops for each Locus


All Pops.

Locus
EST
Locus2
Locus3

Ht

Mean He Mean Ho
0.783
0.776
0.858
0.763
0.753
0.808
0.751
0.747
0.633

Fis
-0.106
-0.073
0.152
Mean
SE

-0.009
0.081

Percentage of Polymorphic Loci


Population
RVA
RVB
LKA
LKB
Mean
SE

%P
100.00%
100.00%
100.00%
100.00%
100.00%
0.00%

Na = No. of Different Alleles


Ne = No. of Effective Alleles = 1 / (Sum pi^2)
I = Shannon's Information Index = -1* Sum (pi * Ln (pi))
Ho = Observed Heterozygosity = No. of Hets / N
He = Expected Heterozygosity = 1 - Sum pi^2
uHe = Unbiased Expected Heterozygosity = (2N / (2N-1)) * He
F = Fixation Index = (He - Ho) / He = 1 - (Ho / He)
Where pi is the frequency of the ith allele for the population & Sum pi^2 is the sum of the

Fis = (Mean He - Mean Ho) / Mean He


Fit = (Ht - Mean Ho) / Ht
Fst = (Ht - Mean He) / Ht
Nm = [(1 / Fst) - 1] / 4
Key: Mean He = Average He across the populations. Mean Ho = Average Ho across the pop
Ht = Total Expected Heterozygosity = 1 - Sum tpi^2 where tpi is the frequency of the ith a

Note:
Mean F-Statistics represent arithmetic averages. See G-Statistics for an alternative mean

dominant Data

bserved Heterozygosity, Expected and Unbiased Expected Heterozygosity, and Fixation Index
He

uHe
0.777
0.710
0.713
0.798
0.773
0.763
0.778
0.811
0.772
0.751
0.719
0.739

He

F
0.790
0.722
0.725
0.812
0.786
0.776
0.791
0.824
0.785
0.764
0.732
0.752

uHe

-0.158
-0.080
0.112
-0.127
-0.034
0.520
-0.071
-0.151
-0.037
-0.065
-0.019
0.008

0.734
0.022

0.746
0.022

-0.042
0.080

0.778
0.010

0.792
0.011

0.119
0.202

0.787
0.012

0.800
0.012

-0.087
0.034

0.737
0.009

0.749
0.009

-0.025
0.021

He

uHe
0.759
0.009

F
0.772
0.010

-0.009
0.053

Fit

Fst
-0.096
-0.060
0.157

0.009
0.012
0.006

Nm
28.222
20.089
41.369

0.000
0.079

0.009
0.002

29.893
6.200

m pi^2 is the sum of the squared population allele frequencies.

erage Ho across the populations.


e frequency of the ith allele for the total & Sum tpi^2 is the sum of the squared total allele frequen

or an alternative mean Fst estimate, calculated based on the average Hs and Ht over loci.

d Fixation Index

ed total allele frequencies.

Ht over loci.

Heterozygosity, Fstatistics and Polymorphism by Locus for Codominant Data


*With Step by Step option
Data Sheet
Sheet1
Data Title
Allozyme
No. Loci
No. Samples
No. Pops.

3
120
4

Sample Size, No. Alleles, No. Effective Alleles, Information Index, Observed Heterozygosity
Pop
RVA

RVB

LKA

LKB

EST
N
Na
Ne
I
Ho
He
uHe
F
N
Na
Ne
I
Ho
He
uHe
F
N
Na
Ne
I
Ho
He
uHe
F
N
Na
Ne
I
Ho
He
uHe
F

30
11
4.489
1.889
0.900
0.777
0.790
-0.158
30
9
4.959
1.863
0.900
0.798
0.812
-0.127
30
9
4.500
1.850
0.833
0.778
0.791
-0.071
30
10
4.018
1.798
0.800
0.751
0.764
-0.065

Locus2
30
8
3.448
1.634
0.767
0.710
0.722
-0.080
30
10
4.412
1.861
0.800
0.773
0.786
-0.034
30
10
5.279
1.968
0.933
0.811
0.824
-0.151
30
8
3.564
1.638
0.733
0.719
0.732
-0.019

Locus3
30
10
3.488
1.708
0.633
0.713
0.725
0.112
30
9
4.225
1.797
0.367
0.763
0.776
0.520
30
10
4.380
1.825
0.800
0.772
0.785
-0.037
30
10
3.838
1.764
0.733
0.739
0.752
0.008

Mean and SE over Pops for each Locus

N
Na
Ne
I
Ho
He
uHe
F

Mean
SE
Mean
SE
Mean
SE
Mean
SE
Mean
SE
Mean
SE
Mean
SE
Mean
SE

EST
Locus2
Locus3
30.000
30.000
30.000
0.000
0.000
0.000
9.750
9.000
9.750
0.479
0.577
0.250
4.491
4.176
3.983
0.192
0.426
0.200
1.850
1.775
1.773
0.019
0.083
0.025
0.858
0.808
0.633
0.025
0.044
0.095
0.776
0.753
0.747
0.010
0.024
0.013
0.789
0.766
0.760
0.010
0.024
0.013
-0.105
-0.071
0.151
0.022
0.030
0.127

Grand Mean and SE over Pops and Loci

N
Na
Ne
I
Ho
He
uHe
F

Mean
SE
Mean
SE
Mean
SE
Mean
SE
Mean
SE
Mean
SE
Mean
SE
Mean
SE

Total
30.000
0.000
9.500
0.261
4.217
0.166
1.800
0.029
0.767
0.044
0.759
0.009
0.772
0.010
-0.009
0.053

F-Statistics and Estimates of Nm over All Pops for each Locus


All Pops.

EST
Ht
Mean He

Locus2
Locus3
0.783
0.763
0.751
0.776
0.753
0.747

Mean Ho

0.858

0.808

0.633

Fis
Fit
Fst

-0.106
-0.096
0.009

-0.073
-0.060
0.012

0.152
0.157
0.006

Nm

28.222

20.089

41.369

Mean
SE
-0.009
0.000
0.009

0.081
0.079
0.002

29.893

6.200

Percentage of Polymorphic Loci


Population
RVA
RVB
LKA
LKB
Mean
SE

%P
100.00%
100.00%
100.00%
100.00%
100.00%
0.00%

Na = No. of Different Alleles


Ne = No. of Effective Alleles = 1 / (Sum pi^2)
I = Shannon's Information Index = -1* Sum (pi * Ln (pi))
Ho = Observed Heterozygosity = No. of Hets / N
He = Expected Heterozygosity = 1 - Sum pi^2
uHe = Unbiased Expected Heterozygosity = (2N / (2N-1)) * He
F = Fixation Index = (He - Ho) / He = 1 - (Ho / He)
Where pi is the frequency of the ith allele for the population & Sum pi^2 is the sum of the

Fis = (Mean He - Mean Ho) / Mean He


Fit = (Ht - Mean Ho) / Ht
Fst = (Ht - Mean He) / Ht
Nm = [(1 / Fst) - 1] / 4
Key: Mean He = Average He across the populations. Mean Ho = Average Ho across the pop
Ht = Total Expected Heterozygosity = 1 - Sum tpi^2 where tpi is the frequency of the ith a

Note:
Mean F-Statistics represent arithmetic averages. See G-Statistics for an alternative mean

nant Data

bserved Heterozygosity, Expected and Unbiased Expected Heterozygosity, and Fixation Index

m pi^2 is the sum of the squared population allele frequencies.

erage Ho across the populations.


e frequency of the ith allele for the total & Sum tpi^2 is the sum of the squared total allele frequen

or an alternative mean Fst estimate, calculated based on the average Hs and Ht over loci.

d Fixation Index

ed total allele frequencies.

Ht over loci.

Allelic Patterns for Codominant Data


*With Step by Step option
Data Sheet
Sheet1
Data Title
Allozyme
No. Loci
No. Samples
No. Pops.

3
120
4

Allelic Patterns across Populations


15.000

0.800

Mean

Na Freq. >=

0.780

10.000

Ne

0.760
0.740

5.000

Heterozygosity

0.720

0.000
RVB

LKA

He

Totals for Allelic Patterns by Populations


RVA
29
19.000
1.000
0.000
3.000

RVB
28
21.000
0.000
0.000
4.000

LKA

LKB

29
23.000
0.000
0.000
4.000

28
18.000
0.000
0.000
4.000

Mean Allelic Patterns Across Populations


EST
Population
Na
Na Freq. >= 5%
Ne
I
No. Private Alleles
No. LComm Alleles (<=25%)
No. LComm Alleles (<=50%)
He

No. Private

No. LComm

LKB

Populations

Population
Na
Na Freq. >= 5%
Ne
No. LComm Alleles (<=25%)
No. LComm Alleles (<=50%)

No. LComm

0.700
RVA

Na

RVA
RVB
LKA
11.000
9.000
6.000
7.000
4.489
4.959
1.889
1.863
1.000
0.000
0.000
0.000
1.000
1.000
0.777
0.798

LKB
9.000
10.000
8.000
6.000
4.500
4.018
1.850
1.798
0.000
0.000
0.000
0.000
1.000
1.000
0.778
0.751

uHe
Locus2
Population
Na
Na Freq. >= 5%
Ne
I
No. Private Alleles
No. LComm Alleles (<=25%)
No. LComm Alleles (<=50%)
He
uHe
Locus3
Population
Na
Na Freq. >= 5%
Ne
I
No. Private Alleles
No. LComm Alleles (<=25%)
No. LComm Alleles (<=50%)
He
uHe
Mean values
Population
Na
Na Freq. >= 5%
Ne
I
No. Private Alleles
No. LComm Alleles (<=25%)
No. LComm Alleles (<=50%)
He
uHe
Standard Error (SE) values
Population
Na
Na Freq. >= 5%
Ne
I
No. Private Alleles

0.790

RVA

0.812

0.791

0.764

RVB
LKA
LKB
8.000
10.000
10.000
7.000
6.000
8.000
3.448
4.412
5.279
1.634
1.861
1.968
0.000
0.000
0.000
0.000
0.000
0.000
0.000
1.000
1.000
0.710
0.773
0.811
0.722
0.786
0.824

8.000
6.000
3.564
1.638
0.000
0.000
1.000
0.719
0.732

RVA
RVB
LKA
LKB
10.000
9.000
10.000
10.000
6.000
8.000
7.000
6.000
3.488
4.225
4.380
3.838
1.708
1.797
1.825
1.764
0.000
0.000
0.000
0.000
0.000
0.000
0.000
0.000
2.000
2.000
2.000
2.000
0.713
0.763
0.772
0.739
0.725
0.776
0.785
0.752

RVA

RVB
9.667
6.333
3.808
1.744
0.333
0.000
1.000
0.734
0.746

RVA

LKA
9.333
7.000
4.532
1.841
0.000
0.000
1.333
0.778
0.792

RVB
0.882
0.333
0.340
0.076
0.333

LKB
9.667
7.667
4.719
1.881
0.000
0.000
1.333
0.787
0.800

LKA
0.333
0.577
0.220
0.022
0.000

9.333
6.000
3.807
1.733
0.000
0.000
1.333
0.737
0.749

LKB
0.333
0.333
0.282
0.044
0.000

0.667
0.000
0.132
0.049
0.000

No. LComm Alleles (<=25%)


No. LComm Alleles (<=50%)
He
uHe

0.000
0.577
0.022
0.022

0.000
0.333
0.010
0.011

0.000
0.333
0.012
0.012

0.000
0.333
0.009
0.009

Na = No. of Different Alleles


Na (Freq >= 5%) = No. of Different Alleles with a Frequency >= 5%
Ne = No. of Effective Alleles = 1 / (Sum pi^2)
I = Shannon's Information Index = -1* Sum (pi * Ln (pi))
No. Private Alleles = No. of Alleles Unique to a Single Population
No. LComm Alleles (<=25%) = No. of Locally Common Alleles (Freq. >= 5%) Found in 25% o
No. LComm Alleles (<=50%) = No. of Locally Common Alleles (Freq. >= 5%) Found in 50% o
He = Expected Heterozygosity = 1 - Sum pi^2
uHe = Unbiased Expected Heterozygosity = (2N / (2N-1)) * He

sity

Na
Na Freq. >= 5%
Ne
I
No. Private Alleles
No. LComm Alleles (<=25%)
No. LComm Alleles (<=50%)
He

5%) Found in 25% or Fewer Populations


5%) Found in 50% or Fewer Populations

Allele List for Codominant Data


Data Sheet
Data Title

Sheet1
Allozyme

No. Loci
No. Samples
No. Pops.
Alleles
80
82
84
86
88
90
92
94
96
98
100
Total No. Alleles

3
120
4
EST

Locus2
1
1
1
1
1
1
1
1
1
1
1
11

Locus3
1
1
1
1
0
1
1
1
1
1
1
10

1
1
1
1
0
1
1
1
1
1
1
10

Summary of Pairwise Population Nei Genetic Distance and Identity


*With Step by Step option
Data Sheet
Sheet1
Data Title
Allozyme
No. Loci
No. Samples
No. Pops.

3
120
4

Pairwise Pops: RVA versus RVB


Locus
EST

RVA
x

Allele

RVB
y

x^2

80
82
84
86
88
90
92
94
96
98
100

0.067
0.017
0.033
0.017
0.017
0.117
0.100
0.117
0.033
0.067
0.417

0.083
0.033
0.000
0.067
0.000
0.150
0.050
0.017
0.083
0.150
0.367

0.004
0.000
0.001
0.000
0.000
0.014
0.010
0.014
0.001
0.004
0.174
0.223

0.007
0.001
0.000
0.004
0.000
0.023
0.003
0.000
0.007
0.023
0.134
0.202

80
82
84
86
90
92
94
96
98
100

0.050
0.000
0.100
0.033
0.083
0.083
0.100
0.000
0.050
0.500

0.067
0.033
0.033
0.033
0.167
0.083
0.033
0.050
0.083
0.417

0.003
0.000
0.010
0.001
0.007
0.007
0.010
0.000
0.003
0.250
0.290

0.004
0.001
0.001
0.001
0.028
0.007
0.001
0.003
0.007
0.174
0.227

80
82
84
86
90
92
94
96

0.050
0.017
0.033
0.083
0.067
0.100
0.017
0.033

0.050
0.000
0.017
0.067
0.100
0.133
0.050
0.067

0.003
0.000
0.001
0.007
0.004
0.010
0.000
0.001

0.003
0.000
0.000
0.004
0.010
0.018
0.003
0.004

Sum over EST


Locus2

Sum over Locus2


Locus3

y^2

98
100

0.100
0.500

0.083
0.433

0.010
0.250
0.287

Sum over Locus3


Jx
Sum over Loci

0.007
0.188
0.237
Jy

0.799
JxJy

(Jx*Jy)^0.5)
0.698
0.729

Nei Genetic Identity


Nei Unbiased Genetic Identity

0.958
1.011

Nei Genetic Distance


Nei Unbiased Genetic Distance

0.043
-0.011

0.665

JxJy / ((Jx *Jy)^0.5)


0.958

Pairwise Pops: RVA versus LKA


Locus
EST

RVA
x

Allele

LKA
y

x^2

80
82
84
86
88
90
92
94
96
98
100

0.067
0.017
0.033
0.017
0.017
0.117
0.100
0.117
0.033
0.067
0.417

0.050
0.050
0.033
0.067
0.000
0.100
0.067
0.100
0.000
0.117
0.417

0.004
0.000
0.001
0.000
0.000
0.014
0.010
0.014
0.001
0.004
0.174
0.223

0.003
0.003
0.001
0.004
0.000
0.010
0.004
0.010
0.000
0.014
0.174
0.222

80
82
84
86
90
92
94
96
98
100

0.050
0.000
0.100
0.033
0.083
0.083
0.100
0.000
0.050
0.500

0.050
0.050
0.017
0.100
0.117
0.083
0.067
0.033
0.117
0.367

0.003
0.000
0.010
0.001
0.007
0.007
0.010
0.000
0.003
0.250
0.290

0.003
0.003
0.000
0.010
0.014
0.007
0.004
0.001
0.014
0.134
0.189

80

0.050

0.067

0.003

0.004

Sum over EST


Locus2

Sum over Locus2


Locus3

y^2

82
84
86
90
92
94
96
98
100

0.017
0.033
0.083
0.067
0.100
0.017
0.033
0.100
0.500

0.017
0.017
0.067
0.133
0.133
0.017
0.050
0.083
0.417

0.000
0.001
0.007
0.004
0.010
0.000
0.001
0.010
0.250
0.287

Sum over Locus3


Jx
Sum over Loci

0.000
0.000
0.004
0.018
0.018
0.000
0.003
0.007
0.174
0.228
Jy

0.799
JxJy

(Jx*Jy)^0.5)
0.688
0.715

Nei Genetic Identity


Nei Unbiased Genetic Identity

0.962
1.018

Nei Genetic Distance


Nei Unbiased Genetic Distance

0.038
-0.018

0.640

JxJy / ((Jx *Jy)^0.5)


0.962

Pairwise Pops: RVB versus LKA


Locus
EST

RVB
x

Allele

LKA
y

x^2

0.083
0.033
0.000
0.067
0.000
0.150
0.050
0.017
0.083
0.150
0.367

0.050
0.050
0.033
0.067
0.000
0.100
0.067
0.100
0.000
0.117
0.417

0.007
0.001
0.000
0.004
0.000
0.023
0.003
0.000
0.007
0.023
0.134
0.202

0.003
0.003
0.001
0.004
0.000
0.010
0.004
0.010
0.000
0.014
0.174
0.222

80
82
84
86
90
92

0.067
0.033
0.033
0.033
0.167
0.083

0.050
0.050
0.017
0.100
0.117
0.083

0.004
0.001
0.001
0.001
0.028
0.007

0.003
0.003
0.000
0.010
0.014
0.007

Sum over EST


Locus2

y^2

80
82
84
86
88
90
92
94
96
98
100

94
96
98
100

0.033
0.050
0.083
0.417

0.067
0.033
0.117
0.367

0.001
0.003
0.007
0.174
0.227

0.004
0.001
0.014
0.134
0.189

80
82
84
86
90
92
94
96
98
100

0.050
0.000
0.017
0.067
0.100
0.133
0.050
0.067
0.083
0.433

0.067
0.017
0.017
0.067
0.133
0.133
0.017
0.050
0.083
0.417

0.003
0.000
0.000
0.004
0.010
0.018
0.003
0.004
0.007
0.188
0.237

0.004
0.000
0.000
0.004
0.018
0.018
0.000
0.003
0.007
0.174
0.228

Sum over Locus2


Locus3

Sum over Locus3


Jx
Sum over Loci

Jy
0.665

JxJy
0.633
Nei Genetic Identity
Nei Unbiased Genetic Identity

0.970
1.033

Nei Genetic Distance


Nei Unbiased Genetic Distance

0.030
-0.033

0.640

(Jx*Jy)^0.5)
0.652

JxJy / ((Jx *Jy)^0.5)


0.970

RVA
x

x^2

Pairwise Pops: RVA versus LKB


Locus
EST

Sum over EST

Allele
80
82
84
86
88
90
92
94
96
98
100

LKB
y
0.067
0.017
0.033
0.017
0.017
0.117
0.100
0.117
0.033
0.067
0.417

0.067
0.017
0.033
0.050
0.000
0.133
0.117
0.033
0.033
0.067
0.450

y^2
0.004
0.000
0.001
0.000
0.000
0.014
0.010
0.014
0.001
0.004
0.174
0.223

0.004
0.000
0.001
0.003
0.000
0.018
0.014
0.001
0.001
0.004
0.203
0.249

Locus2

80
82
84
86
90
92
94
96
98
100

0.050
0.000
0.100
0.033
0.083
0.083
0.100
0.000
0.050
0.500

0.067
0.000
0.033
0.000
0.100
0.133
0.100
0.050
0.033
0.483

0.003
0.000
0.010
0.001
0.007
0.007
0.010
0.000
0.003
0.250
0.290

0.004
0.000
0.001
0.000
0.010
0.018
0.010
0.003
0.001
0.234
0.281

80
82
84
86
90
92
94
96
98
100

0.050
0.017
0.033
0.083
0.067
0.100
0.017
0.033
0.100
0.500

0.033
0.017
0.050
0.017
0.117
0.083
0.100
0.033
0.083
0.467

0.003
0.000
0.001
0.007
0.004
0.010
0.000
0.001
0.010
0.250
0.287

0.001
0.000
0.003
0.000
0.014
0.007
0.010
0.001
0.007
0.218
0.261

Sum over Locus2


Locus3

Sum over Locus3


Jx
Sum over Loci

Jy
0.799

JxJy

(Jx*Jy)^0.5)
0.776
0.795

Nei Genetic Identity


Nei Unbiased Genetic Identity

0.976
1.024

Nei Genetic Distance


Nei Unbiased Genetic Distance

0.024
-0.024

0.790

JxJy / ((Jx *Jy)^0.5)


0.976

Pairwise Pops: RVB versus LKB


Locus
EST

RVB
x

Allele
80
82
84
86
88

LKB
y
0.083
0.033
0.000
0.067
0.000

x^2
0.067
0.017
0.033
0.050
0.000

y^2
0.007
0.001
0.000
0.004
0.000

0.004
0.000
0.001
0.003
0.000

90
92
94
96
98
100

0.150
0.050
0.017
0.083
0.150
0.367

0.133
0.117
0.033
0.033
0.067
0.450

0.023
0.003
0.000
0.007
0.023
0.134
0.202

0.018
0.014
0.001
0.001
0.004
0.203
0.249

80
82
84
86
90
92
94
96
98
100

0.067
0.033
0.033
0.033
0.167
0.083
0.033
0.050
0.083
0.417

0.067
0.000
0.033
0.000
0.100
0.133
0.100
0.050
0.033
0.483

0.004
0.001
0.001
0.001
0.028
0.007
0.001
0.003
0.007
0.174
0.227

0.004
0.000
0.001
0.000
0.010
0.018
0.010
0.003
0.001
0.234
0.281

80
82
84
86
90
92
94
96
98
100

0.050
0.000
0.017
0.067
0.100
0.133
0.050
0.067
0.083
0.433

0.033
0.017
0.050
0.017
0.117
0.083
0.100
0.033
0.083
0.467

0.003
0.000
0.000
0.004
0.010
0.018
0.003
0.004
0.007
0.188
0.237

0.001
0.000
0.003
0.000
0.014
0.007
0.010
0.001
0.007
0.218
0.261

Sum over EST


Locus2

Sum over Locus2


Locus3

Sum over Locus3


Jx
Sum over Loci

0.665
JxJy

(Jx*Jy)^0.5)
0.700
0.725

Nei Genetic Identity


Nei Unbiased Genetic Identity

0.965
1.020

Nei Genetic Distance


Nei Unbiased Genetic Distance

0.035
-0.020

Pairwise Pops: LKA versus LKB

Jy
0.790

JxJy / ((Jx *Jy)^0.5)


0.965

Locus
EST

LKA
x

Allele

LKB
y

x^2

0.050
0.050
0.033
0.067
0.000
0.100
0.067
0.100
0.000
0.117
0.417

0.067
0.017
0.033
0.050
0.000
0.133
0.117
0.033
0.033
0.067
0.450

0.003
0.003
0.001
0.004
0.000
0.010
0.004
0.010
0.000
0.014
0.174
0.222

0.004
0.000
0.001
0.003
0.000
0.018
0.014
0.001
0.001
0.004
0.203
0.249

80
82
84
86
90
92
94
96
98
100

0.050
0.050
0.017
0.100
0.117
0.083
0.067
0.033
0.117
0.367

0.067
0.000
0.033
0.000
0.100
0.133
0.100
0.050
0.033
0.483

0.003
0.003
0.000
0.010
0.014
0.007
0.004
0.001
0.014
0.134
0.189

0.004
0.000
0.001
0.000
0.010
0.018
0.010
0.003
0.001
0.234
0.281

80
82
84
86
90
92
94
96
98
100

0.067
0.017
0.017
0.067
0.133
0.133
0.017
0.050
0.083
0.417

0.033
0.017
0.050
0.017
0.117
0.083
0.100
0.033
0.083
0.467

0.004
0.000
0.000
0.004
0.018
0.018
0.000
0.003
0.007
0.174
0.228

0.001
0.000
0.003
0.000
0.014
0.007
0.010
0.001
0.007
0.218
0.261

Sum over EST


Locus2

Sum over Locus2


Locus3

y^2

80
82
84
86
88
90
92
94
96
98
100

Sum over Locus3


Jx
Sum over Loci

Jy
0.640

JxJy

(Jx*Jy)^0.5)
0.680
0.711

0.790

JxJy / ((Jx *Jy)^0.5)


0.957

Nei Genetic Identity


Nei Unbiased Genetic Identity

0.957
1.012

Nei Genetic Distance


Nei Unbiased Genetic Distance

0.044
-0.012

Where x represents the allele frequencies in pop1. y represents the allele frequencies in p
xUb and yUb are calculated each locus as: xUb = (2Nx * Sum x^2-1) / (2Nx - 1); yUb=(2Ny *
Jx is the sum of x^2 over loci. Jy is the sum of y^2 over loci. JxJy is the sum of xy over loci.
Nei Genetic Identity = JxJy / (Jx * Jy)^0.5. Nei Unbiased Genetic Identity = Jxy / (JxUb * JyUb
Nei Genetic Distance = -1 * Ln(Nei Identity). Nei Unbiased Genetic Distance = -1 * Ln(Nei U
As recommended by Nei (1978), negative Unbiased Nei Genetic Distances are converted to

xy

2Nx

xUb

2Ny

yUb

0.006
0.001
0.000
0.001
0.000
0.018
0.005
0.002
0.003
0.010
0.153
0.197

60

0.210

60

0.188

0.003
0.000
0.003
0.001
0.014
0.007
0.003
0.000
0.004
0.208
0.244

60

0.278

60

0.214

0.003
0.000
0.001
0.006
0.007
0.013
0.001
0.002

0.008
0.217
0.257

60

JxJy

60

JxUb
0.762

0.698

xy

0.275

0.224
JyUb
0.625

(JxUb*JyUb)^0.5)
0.690

JxJy / ((JxUb* JyUb)^0.5)


1.011

2Nx

2Ny

xUb

yUb

0.003
0.001
0.001
0.001
0.000
0.012
0.007
0.012
0.000
0.008
0.174
0.218

60

0.210

60

0.209

0.003
0.000
0.002
0.003
0.010
0.007
0.007
0.000
0.006
0.183
0.220

60

0.278

60

0.176

0.003

0.000
0.001
0.006
0.009
0.013
0.000
0.002
0.008
0.208
0.251

60

JxJy

0.004
0.002
0.000
0.004
0.000
0.015
0.003
0.002
0.000
0.018
0.153
0.201
0.003
0.002
0.001
0.003
0.019
0.007

60

JxUb
0.762

0.688

xy

0.275

0.215
JyUb
0.600

(JxUb*JyUb)^0.5)
0.676

JxJy / ((JxUb* JyUb)^0.5)


1.018

2Nx

2Ny

xUb

60

0.188

yUb

60

0.209

0.002
0.002
0.010
0.153
0.202

60

0.214

60

0.176

0.003
0.000
0.000
0.004
0.013
0.018
0.001
0.003
0.007
0.181
0.231

60

0.224

60

0.215

JxJy

JxUb
0.625

0.633

xy
0.004
0.000
0.001
0.001
0.000
0.016
0.012
0.004
0.001
0.004
0.188
0.231

JyUb
0.600

(JxUb*JyUb)^0.5)
0.613

JxJy / ((JxUb* JyUb)^0.5)


1.033

2Nx

2Ny

xUb

60

0.210

yUb

60

0.236

0.003
0.000
0.003
0.000
0.008
0.011
0.010
0.000
0.002
0.242
0.279

60

0.278

60

0.268

0.002
0.000
0.002
0.001
0.008
0.008
0.002
0.001
0.008
0.233
0.266

60

0.275

60

0.248

JxJy

JxUb
0.762

0.776

xy
0.006
0.001
0.000
0.003
0.000

JyUb
0.753

(JxUb*JyUb)^0.5)
0.757

JxJy / ((JxUb* JyUb)^0.5)


1.024

2Nx

2Ny

xUb

yUb

0.020
0.006
0.001
0.003
0.010
0.165
0.214

60

0.188

60

0.236

0.004
0.000
0.001
0.000
0.017
0.011
0.003
0.003
0.003
0.201
0.243

60

0.214

60

0.268

0.002
0.000
0.001
0.001
0.012
0.011
0.005
0.002
0.007
0.202
0.243

60

0.224

60

0.248

JxJy
0.700

JxUb
0.625
(JxUb*JyUb)^0.5)
0.686

JyUb
0.753
JxJy / ((JxUb* JyUb)^0.5)
1.020

xy

2Nx

xUb

2Ny

yUb

0.003
0.001
0.001
0.003
0.000
0.013
0.008
0.003
0.000
0.008
0.188
0.228

60

0.209

60

0.236

0.003
0.000
0.001
0.000
0.012
0.011
0.007
0.002
0.004
0.177
0.216

60

0.176

60

0.268

0.002
0.000
0.001
0.001
0.016
0.011
0.002
0.002
0.007
0.194
0.236

60

0.215

60

0.248

JxJy
0.680

JxUb
0.600
(JxUb*JyUb)^0.5)
0.672

JyUb
0.753
JxJy / ((JxUb* JyUb)^0.5)
1.012

ele frequencies in pop 2.


2Nx - 1); yUb=(2Ny * Sum y^2-1) / (2Ny - 1)
sum of xy over loci. JxUb is the sum of xUb over loci. JyUb is the sum of yUb over loci.
= Jxy / (JxUb * JyUb)^0.5
ance = -1 * Ln(Nei Unbiased Identity).
ces are converted to zero with associated warnings in subsequent outputs!

over loci.

Allozyme Sheet1
Pairwise Population Matrix of Nei Genetic Distance
RVA
RVB
LKA
LKB
0.000
RVA
0.043
0.000
RVB
0.038
0.030
0.000
LKA
0.024
0.035
0.044
0.000 LKB

Allozyme Sheet1
Pairwise Population Matrix of Nei Genetic Identity
RVA
RVB
LKA
LKB
1.000
RVA
0.958
1.000
RVB
0.962
0.970
1.000
LKA
0.976
0.965
0.957
1.000 LKB

Allozyme Sheet1
Pairwise Population Matrix of Nei Unbiased Genetic Distance
RVA
RVB
LKA
LKB
0.000
RVA
0.000
0.000
RVB
0.000
0.000
0.000
LKA
0.000
0.000
0.000
0.000 LKB

Allozyme Sheet1
Pairwise Population Matrix of Nei Unbiased Genetic Identity
RVA
RVB
LKA
LKB
1.000
RVA
1.011
1.000
RVB
1.018
1.033
1.000
LKA
1.024
1.020
1.012
1.000 LKB

Warning!
Warning!
Warning!
Warning!
Warning!
Warning!

Negative
Negative
Negative
Negative
Negative
Negative

Unbiased
Unbiased
Unbiased
Unbiased
Unbiased
Unbiased

Nei
Nei
Nei
Nei
Nei
Nei

Genetic
Genetic
Genetic
Genetic
Genetic
Genetic

Distance
Distance
Distance
Distance
Distance
Distance

of
of
of
of
of
of

-0,011
-0,018
-0,033
-0,024
-0,020
-0,012

between
between
between
between
between
between

pops
pops
pops
pops
pops
pops

RVA and RVB co


RVA and LKA co
RVB and LKA co
RVA and LKB co
RVB and LKB co
LKA and LKB co

pops
pops
pops
pops
pops
pops

RVA and RVB converted to zero!


RVA and LKA converted to zero!
RVB and LKA converted to zero!
RVA and LKB converted to zero!
RVB and LKB converted to zero!
LKA and LKB converted to zero!

Allozyme Sheet1
Pairwise Population Fst Values
RVA
RVB
LKA
LKB
0.000
RVA
0.007
0.000
RVB
0.007
0.004
0.000
LKA
0.004
0.006
0.008
0.000 LKB
Fst Values below diagonal.