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winCATS Planar Chromatography Manager

LABORATORIUM BIOFARMAKA
FAKULTAS FARMASI
UNIVERSITAS HASANUDIN
Analysis Report
SOP document
Validated
Description :

Design

Analysis

C:\CAMAG\winCATS\Data\Praktikan Fitokimia Golongan


Senin 2013\Fito Kam
is 2015\kelompok 6 Bayam duri.cna

Created/used by
Current user

camag
camag

Saturday, January 01, 2005 12:50:21 AM

Stationary phase
Executed by
Plate size (X x Y)
Material
Manufacturer
Batch
GLP code
Pre-washing
Modification

camag
Saturday, January 01, 2005 12:44:08 AM
5.0 x 10.0 cm
HPTLC plates silica gel 60 F 254
E. MERCK KGaA

No
No

Definitions - Screening
Executed by

camag

Saturday, January 01, 2005 12:47:37 AM

Samples
awal
Heksan
BJA
Substance name
spot 1
spot 2

Rf

Window size

0.94
0.75

0.500
0.500

Manufacturer

Batch
number

Expiry date

Product
number

Detection - CAMAG TLC Scanner 3


Information
Application position
Solvent front position

11.5 mm
94.0 mm

Instrument

CAMAG TLC Scanner 3 "Scanner3_140717" S/N 140717 (1.14.28)


camag
Saturday, January 01, 2005 12:44:08 AM
3
11.5 mm
10.0 mm
9.0 mm
94.0 mm
4.00 x 0.30 mm, Micro
Light
20 mm/s
100 m/step

Executed by
Number of tracks
Position of first track X
Distance between tracks
Scan start pos. Y
Scan end pos. Y
Slit dimensions
Optimize optical system
Scanning speed:
Data resolution:

User : camag
Saturday, January 01, 2005 12:50:43 AM

Approved :. . . . . . . . . . . . . . . . . . .
Report ID : 07D5010107003215

SN 1410W024, V1.4.3
Page 1 of 5

winCATS Planar Chromatography Manager


Measurement Table
Wavelength
Lamp
Measurement Type
Measurement Mode
Optical filter
Detector mode
PM high voltage

254
D2 & W
Remission
Absorption
Second order
Automatic
334 V

Detector properties
Y-position for 0 adjust
Track # for 0 adjust
Analog Offset
Sensitivity

9.0 mm
0
10%
Automatic (29)

Integration
Properties
Data filtering
Baseline correction
Peak threshold min. slope
Peak threshold min. height
Peak threshold min. area
Peak threshold max. height
Track start position
Track end position
Display scaling

Savitsky-Golay 7
Lowest Slope
5
10 AU
50
990 AU
11.5 mm
94.0 mm
Automatic

All tracks at Wavelength

500
[ AU ]
400
350
300
250
200
150
50

100

[ mm ]

50

40

35

0.00
30
0.20

25
20

0.40
15

0.60

10
[ Rf ]

5
1.00 0

User : camag
Saturday, January 01, 2005 12:50:43 AM

Approved :. . . . . . . . . . . . . . . . . . .
Report ID : 07D5010107003215

SN 1410W024, V1.4.3
Page 2 of 5

winCATS Planar Chromatography Manager


Track 1, ID:
500

500

450

450

400

400

350

350

300

300

250

250

200

200

150

150

100

100

50

50

0
-0.14

0.06

Start
Peak Rf
1
0.71
2
0.73
3
0.87

0.26

Start
Height
3.6
11.2
0.9

0.46

Max
Rf
0.72
0.75
0.93

Max
Height
13.5
26.5
166.3

0.66

Max
%
6.56
12.84
80.60

0.86

End
Rf
0.73
0.78
0.98

1.06

End
Height
10.4
3.9
17.5

spot 1 @ 254 nm

0
-0.14

0.06

Area
%
1.58
8.35
90.06

Area
131.3
692.3
7465.3

0.26

0.46

0.66

spot 2 @ 254 nm

0.86

1.06

Assigned substance
unknown *
spot 2
spot 1

Track 2, ID:
400

400

350

350

300

300

250

250

200

200

150

150

100

100

50

50

1spot 1 @ 254 nm

0
-0.14

0.06

Start
Peak Rf
1
0.86
2
0.93

0.26

Start
Height
6.7
129.9

0.46

Max
Rf
0.92
0.94

Max
Height
131.1
132.2

0.66

Max
%
49.80
50.20

0.86

End
Rf
0.93
0.99

1.06

End
Height
130.0
20.0

0
-0.14

0.06

Area
%
44.07
55.93

Area
3405.1
4322.0

0.26

0.46

0.66

0.86

1.06

Assigned substance
unknown *
spot 1

Track 3, ID:
400

400

350

350

300

300

250

250

200

200

150

150

100

100

50

50

0
-0.14

0.06

Start
Peak Rf
1
0.85

0.26

Start
Height
0.4

0.46

Max
Rf
0.94

0.66

Max
Max
Height
%
111.5 100.00

User : camag
Saturday, January 01, 2005 12:50:43 AM

0.86

End
Rf
0.96

1.06

End
Height
61.8

spot 1 @ 254 nm

0
-0.14

Area
6625.4

0.06

Area
%
100.00

Approved :. . . . . . . . . . . . . . . . . . .
Report ID : 07D5010107003215

0.26

0.46

0.66

0.86

1.06

Assigned substance
spot 1

SN 1410W024, V1.4.3
Page 3 of 5

winCATS Planar Chromatography Manager


Spectrum scan
Executed by
Mode
Slit dimensions
Optimize optical system
Scanning speed
Data resolution
Reference spectrum, pos X
Reference spectrum, pos Y

camag
Saturday, January 01, 2005 12:50:21 AM
All detected peaks
6.00 x 0.30 mm, Micro
Resolution
100 nm/s
1 nm/step
11.5 mm
9.0 mm

Measurement Table
Lamp
Start wavelength
End wavelength
Measurement type
Measurement Mode
Optical filter
Detector Mode

D2 & W
200 nm
700 nm
Remission
Absorption
Second order
Automatic

Detector properties
Y-position for 0 adjust
Track # for 0 adjust
Analog Offset
Sensitivity

0.0 mm
0
10%
Automatic (25)

spot 1 on all Tracks


100.0

100.0

[ AU ]

[ AU ]

80.0

80.0

70.0

70.0

60.0

60.0

50.0

50.0

40.0

40.0

30.0

30.0

20.0

20.0

10.0

10.0

0.0
0.0
200.0 250.0 300.0 350.0 400.0 450.0 500.0 550.0 600.0 [ nm ] 700.0

T
1
2
3

Rf
0.93 Rf
0.94 Rf
0.94 Rf

Substance
spot 1
spot 1
spot 1

Max. @
200 nm
200 nm
200 nm

spot 2 on all Tracks


100.0

100.0

[ AU ]

[ AU ]

80.0

80.0

70.0

70.0

60.0

60.0

50.0

50.0

40.0

40.0

30.0

30.0

20.0

20.0

10.0

10.0

0.0
0.0
200.0 250.0 300.0 350.0 400.0 450.0 500.0 550.0 600.0 [ nm ] 700.0

T
1

Rf
Substance
0.75 Rf spot 2

User : camag
Saturday, January 01, 2005 12:50:43 AM

Max. @
200 nm

Approved :. . . . . . . . . . . . . . . . . . .
Report ID : 07D5010107003215

SN 1410W024, V1.4.3
Page 4 of 5

winCATS Planar Chromatography Manager


Evaluation results
Evaluation Sequence
Track
1
2
3

Track type
Sample
Sample
Sample

Vial
1
1
1

Sample ID

Table of substances
Substance
spot 1
spot 2

Position
MD mm
88.7
73.2

Tracks
1 2 3
A A A
A

User : camag
Saturday, January 01, 2005 12:50:43 AM

Approved :. . . . . . . . . . . . . . . . . . .
Report ID : 07D5010107003215

SN 1410W024, V1.4.3
Page 5 of 5