1.

The graph below shows the effect of changing the substrate concentration on an enzyme
controlled reaction.
4 0 % s u b s tra te
2 0 % s u b s tra te
1 0 % s u b s tra te
A m o u n t o f p ro d u c t fo rm e d
5 % s u b s tra te

2 .5 % s u b s tra te
0 % s u b s tra te
T im e

What is the correct interpretation of these data?

A.

The rate of reaction increases continuously with increase in substrate concentration.

B.

The rate of reaction decreases continuously with increase in substrate concentration.

C.

The rate of reaction increases up to a point and then remains constant.

D.

The rate of reaction is not affected by any change in the substrate concentration.
(Total 1 mark)

2.

What is an active site?

A.

The part of an enzyme that binds only to the product molecules.

B.

The sequence of amino acids responsible for the catalytic activity of enzymes.

C.

The sequence of amino acids responsible for the structure of an enzyme.

D.

The specific area responsible for the activity of all proteins.

(Total 1 mark)

3.

Consider the metabolic pathway shown below.

1

V WXYZ
If there is end-product inhibition, which product would inhibit which enzyme?
Product

Enzyme

A.

B.

C.

D.

1
(Total 1 mark)

4.
Which graph shows the relationship between the substrate concentration and the rate of
an enzyme controlled reaction?
B.

R a te o f
re a c tio n

R a te o f
re a c tio n

A .

S u b s tra te
c o n c e n tra tio n

S u b s tr a te
c o n c e n tra tio n
C.

D .

(Total 1 mark)

5.

The graph below shows enzyme activity plotted against temperature. What is happening at point
I?

I
E n z y m e a c tiv ity / a rb itra ry u n its

T e m p e r a tu r e / C
A.

The enzyme is being denatured.

B.

pH changes are slowing the reaction.

C.

The concentration of the substrate remains constant.

D.

The reaction is increasing in speed.

(Total 1 mark)

6.
reaction?

Which diagram shows how enzymes change the activation energy in an endergonic
A .

B.

Y
X

Y
E n e rg y

E n e rg y

P ro g re s s o f re a c tio n

P ro g re s s o f re a c tio n

C.

D .

Y
E n e rg y

E n e rg y

P ro g re s s o f re a c tio n

Key: X = without enzyme

P ro g re s s o f re a c tio n

Y = with enzyme
(Total 1 mark)

7.

The diagram below shows the energy levels of a reaction in the presence or absence of an
enzyme. What is the best explanation of the different energy levels labelled I, II and III?

E n e rg y le v e l

I
II
E n e rg y o f
re a c ta n ts

III

E n e rg y o f p ro d u c ts
P ro g re s s o f re a c tio n

II

III

A.

Absence of an enzyme

Presence of an enzyme

Endergonic reaction

B.

Presence of an enzyme

Absence of an enzyme

Exergonic reaction

C.

Absence of an enzyme

Presence of an enzyme

Exergonic reaction

D.

Presence of an enzyme

Absence of an enzyme

Endergonic reaction
(Total 1 mark)

8.

Which statement describes how allosteric enzymes work?

4

Reversible

Competitive
inhibition

End-product
inhibition

Active and
inactive forms

A.
B.
C.
D.
Key:

= yes

= no
(Total 1 mark)

9.

What determines the specificity of an enzyme for its substrate?

A.

The temperature at which it is operating

B.

The optimum pH of the enzymes

C.

The concentration of the substrate

D.

The structure of the enzyme molecule

(Total 1 mark)

10.

According to the induced fit model of enzyme function, which of the following statements is
correct?
A.

Active sites on enzymes are specific to a single substrate.

B.

The shape of the active site can be changed by the binding of an allosteric inhibitor.

C.

The binding of the substrate changes the shape of the active site slightly.

D.

Competitive inhibitors can change the shape of enzymes.

(Total 1 mark)

11.

Which of the following could cause denaturation of an enzyme?

A.

Substrate concentration

B.

A competitive inhibitor

C.

High temperature

D.

Low salt concentration

(1)

12.

The reaction below shows the energy changes in a chemical reaction.

What would happen to the changes in energy if this reaction was controlled by an enzyme?
A.

I would increase.

B.

II would decrease.

C.

I and IV would decrease.

D.

II and III would decrease.

(Total 1 mark)

13.

The graphs below show the energy changes during endergonic and exergonic reactions, with
and without enzymes.

Which line represents an endergonic reaction, without an enzyme?

A.

B.

II

C.

III

D.

IV
(Total 1 mark)

14.

What effect do enzymes have on the activation energy of exergonic and endergonic reactions?
6

Activation energy of Activation energy of

exergonic reactions endergonic reactions
A.

increases

increases

B.

decreases

decreases

C.

increases

decreases

D.

decreases

increases
(Total 1 mark)

15.

What can reduce the effect of a competitive inhibitor of an enzyme?

A.

Decrease the temperature at which the reaction takes place.

B.

Increase the temperature at which the reaction takes place.

C.

Increase the substrate concentration.

D.

Add a non-competitive inhibitor.

(Total 1 mark)