PHYSIOLOGY LAB PRACTICALS

4. Filter Paper (or Cotton)

Experiment V: Differentiating Isotonic from Isometric

Contraction
A. Isotonic Contraction
Materials:
1. Dumbell

B. Hemoglobin Determination
Materials
1. Sahli-Hellige Hemometer

B. Isometric Contraction
Materials:
1. Hand grip
2. Lancet

Experiment VI: Hematology

For all experiments, requires:

3. Cotton balls (with alcohol)

4. Distilled Water

1. Cotton balls (with alcohol)

2. Syringes

C. Bleeding Time and Clotting Time

Materials:
1. Lancet
2. Glass Slide (plain)

A. RBC and WBC Count

Materials:
1. Hemocytometer

3. Pin or Matchstick
4. Absorbent Paper (Filter paper)
5. Timer

2a. Saline Solution

(For RBC)

D. Blood Typing
Materials:
1. 2 test tubes (containing saline
solution)
2. Glass Slide with 2 concavities

2b. Or 1% Acetic Acid (For WBC)

3. Microscope

3. Matchsticks

4. Blood typing sera

1b. Or microhematocrit
pipette, heparinized

2. Centrifuge

E. Cross Matching
Materials:
1. 2 test tubes (containing saline solution)
2. Glass Slide (plain)
3. Applicator Stick

Experiment VII: Respiratory Experiments

A. Static Lung Volume
Materials:
1. Spirometer

4. Centrifuge

B. Recording Respiratory Movements

Materials:
1. Pneumograph

F. RBC Fragility Test (Osmotic Fragility Test)

Materials:
1. Big rack with 12 test tubes containing different
concentrations of saline solution
2. Kymograph
3. Timer
C. Duration of Voluntary Apnea
Materials:

2. 0.5% stock solution

3. Distilled water
4. Dropper

G. Hematocrit Determination
Materials:
1a. Wintrobe tube containing anticoagulant

1. Pneumograph
2. Kymograph
3. Timer
D. Measurement of Peak Expiratory Flow Rate
Materials:
1. Peak flow meter

E. Examination of Chest and Lungs

Materials:
1. Stethoscope

2. Graduated Cylinder

3. Dip Stick

Experiment IX: Examination of the Heart and Pulses

Materials:
1. Stethoscope
2. Timer
Experiment X: Arterial Blood Pressure Determination
A. Palpatory and Auscultatory Methods
Materials:
1. Stethoscope
2. Sphygmomamometer

B. Cold Pressor Test

Materials:
1. Stethoscope
2. Sphygmomamometer
3. Pain Perception Scale

4. Urinometer

Experiment XII: General Senses

I. Cutaneous Senses
A. Punctiform Distribution of Sensory Receptors
1. Large Handkerchief
2. Graphing paper with 10x10 mm square hole
3. Fine bristle/Horse hair
4. Pin head

4. Bucket of ice cold water

(0-5 degrees Celsius)

Experiment XI: Kidney Function Test

Materials:
1. Clean small bottles
for urine collection

5. Container with ice cold water

6. 4 colored pens/Pencils
B1. Attributes of Sensation: Contrast
Materials:
1. Large Handkerchief
2. Beaker 1: filled with warm water
3. Beaker 2: filled with ice water
4. Beaker 3: filled with tap water

B2. Attributes of Sensation: Summation

Materials:
1. Large Handkerchief
2. Basin filled with warm water
B3. Attributes of Sensation: Adaptation
Materials:
1. Large Handkerchief
2. Basin filled with warm water
3. Piece of cork

E2. Synthetic Sense: Vibration Sense (Pallesthesia)

Materials:
1. Tuning fork

2. Timer
II. Muscle and Joint Senses (Proprioception)
A. Static Position Sense
Materials: none

4. Thermometer
B. Kinesthesia
Materials:
1. Protractor
Experiment XIII: Special Senses: Vision

C. Pressure Sense
Materials:
1. Large Handkerchief
2. Small bucket (or jar) filled with water and sand

D. Aristotles Experiment
Materials:
1. Large Handkerchief
2. Marble or any rounded object
E1. Synthetic Sense: Two-Point Discrimination
Materials:
1. Large Handkerchief
2. Double pointed Compass

3. Ruler
E2. Synthetic Sense: Stereogenesis
Materials:
1. Large Handkerchief
2. 3 Different Common Objects (e.g. coin, pen, key)

A. Visual Acuity for Far Vision

Materials:
1. Snellen chart

2. Eye Occluder/Pinhole

B. Visual Acuity for Near Vision

Materials:

G. Blind Spot
Materials:
1. Chin Rest

1. Jaeger Chart

2. Paper for blind spot test

C. Near Point of Distinct Vision

Materials:
1. Jaeger Chart
D. Accomodation Reflex
Materials: none

3. Pencil wrapped with white paper

H. Depth Perception
Materials:
1. Depth perception kit

E. Color Blindness
Materials:
1. Dvorine or Ishihara Plates

2. Ruler or tape measure

Experiment XIV: Taste. Smell, Hearing and Equilibrium
I. Taste
Materials:
1. Piece of gauze
2. Sugar in solution and in crystal form
3. Timer
II. Smell
A. Method of Testing
Materials:
1. Large handkerchief
2. Test substance (coffee, mint, onion, garlic)

F. Holmgren-Type Color Vision Test

Materials:

B. Adaptation of Olfactory End Organ

Materials:
1. Cotton (to plug nostrils)
2. Large handkerchief
3. Test substance (tincture of iodine)
4. Timer

1. Holmgren-type color
vision test kit

III. Hearing (Should be done in a quiet room)

2. Timer

A. Threshold of Hearing
Materials:
1. Large handkerchief
2. Clock or any object with ticking sound
3. Cotton

4. Meter stick

4. Reflex hammer - jaw, knee, ankle and plantar reflexes

B. Intensity of Sound
Materials:
1. Sound meter

C. Localization of Sound
Materials:
1. Large handkerchief
2. Two coins/any object with ticking sound
D. Weber Test
Materials:
1. Tuning forks
2. Cotton balls
E. Rinne Test
Materials:
1. Tuning forks
2. Timer

Experiment XVII: Motor System Examination

Materials:
1. Reflex Hammer

Special Mention to Back Row Notes and Physio Manual

for the lacking experiments
Prepared by Jian Narag
Photos by: Kay Ramos (and internet)
Model: Deus Mata
Words of Wisdom from Slave Driver: Pio Savaitnisagon;

ARAL!

Experiment IV. Equilibrium

A. Simple test for the Horizontal Canals
Materials:
1. Meter stick or cane
Experiment XV: Temperature Regulation
Materials:
1. Digital thermometer
2. Reading material
3. Timer
4. Snacks
5. Cotton balls with alcohol
Experiment XVI: Reflexes in Man
Materials:
1. Cotton wisp - corneal reflex
2. Penlight
pupillary light
reflex

3. Tongue depressor
- gag reflex

Deus Agrees.
Good luck with the Practicals guys!