ABSTRACT

DETECTION OF ENTEROPATHOGENIC Escherichia coli (EPEC) O86,

O114, O125, O127, O128 FROM CHILDREN UNDER-FIVE YEARS WITH
DIARRHEA ATTENDING THE HOSPITALITY PRIMARY HEALTH CARE IN
PEKANBARU

By
SABELLA GUSTIKA VERNANDA
Diarrheal diseases remain one of the leading cause of childhood morbidity
and mortality in most developing countries, with Enteropathogenic Escherichia
coli (EPEC) being one of the most important aetiologic agents of children underfive years diarrhea in many of these countries. In Indonesia especially
Pekanbaru, the epidemiology of these EPEC have not been well studied. During
the period from April 2014 to September 2014, 47 stool sample were collected
from children under-five years with acute diarrhea in the hospitality primary
health care in Pekanbaru and the examination has been done in Microbiology
Laboratory of Medical Faculty Riau University. The Escherichia coli were
isolated and diagnosed by standard microbiological methods. The isolates
confirmed to be Esherichia coli were subjected to a slide agglutination test with
Escherichia coli polyvalent antisera. The following typing sera was used
polyvalent 3 (O86, O114, O125, O127, O128). This study was a descriptive study
using a consecutive sampling method to describe the prevalence of
Enteropathogenic Escherichia coli (EPEC) O86, O114, O125, O127, O128.
Based on gender and age from all specimens that were collected, the diarrhea
mostly found in male (59.5%), and in 12-59 months patients (93.6%). Most of the
patients who had diarrhea experiencing fever (57.4%) and mild-moderate
dehydration (74.5%). Out of the 47 stool samples, 1 isolate (2.2%) were positive
for EPEC O86, O114, O125, O127, O128. The isolate was recovered from the 19
months female who experienced fever and mild-moderate dehydration.

Key words: diarrhea, enteropathogenic Escherichia coli, EPEC, children underfive years, hospitality primary health care

ABSTRAK
PEMERIKSAAN ENTEROPATHOGENIC Escherichia coli (EPEC) O86,
O114, O125, O127, O128 PADA BALITA DENGAN DIARE DI
PUSKESMAS RAWAT INAP KOTA PEKANBARU
Oleh
SABELLA GUSTIKA VERNANDA

Diare merupakan penyebab utama morbiditas dan mortalitas pada balita di

banyak negara berkembang, dengan Enteropathogenic Echerichia coli (EPEC)
sebagai salah satu etiologi terpenting pada balita dengan diare di banyak negara
berkembang tersebut. Di Indonesia, khususnya Pekanbaru, epidemiologi dari
EPEC masih belum diteliti secara mendalam. Selama kurun waktu April 2014
sampai dengan September 2014, berhasil dikumpulkan sampel tinja sebanyak 47
sampel dari balita dengan diare akut di Puskesmas Rawat Inap Kota Pekanbaru.
Pemeriksaan kultur tinja dilakukan di Laboratorium Mikrobiologi Fakultas
Kedokteran Universitas Riau. Escherichia coli diidentifikasi dan diisolasi
menggunakan metode mikrobiologi standar. Isolat yang positif Escherichia coli
kemudian di uji kembali menggunakan tes slide aglutinasi dengan antiserum
polyvalent Escherichia coli. Polyvalent yang digunakan terdiri dari O86, O114,
O125, O127, O128. Penelitian ini merupakan penelitian deskriptif dengan metode
consecutive sampling untuk mengetahui gambaran prevalensi dari EPEC O86,
O114, O125, O127, O128. Berdasarkan umur dan jenis kelamin dari seluruh 47
sampel yang berhasil dikumpulkan, diare terbanyak ditemukan pada balita lakilaki (59,5%) dan dalam rentang usia 12-59 bulan (93,6%). Mayoritas pasien
mengalami demam (57,4%) dan dehidrasi ringan-sedang (74,5%). Dari seluruh 47
sampel, didapatkan 1 isolat (2,2%) yang positif EPEC O86, O114, O125, O127,
O128. Isolat ditemukan pada tinja balita perempuan yang mengalami demam dan
dehidrasi ringan-sedang.
Kata kunci: diare, enteropathogenic Escherichia coli, EPEC, balita, puskesmas
rawat inap