journal of Natural

Vol. 12, No. 2, 2012

THE POTENT OF METHANOL EXTRACTS OF CASHEW

(ANACARDIUM OCCIDENTALE L.) AGAINST METHICILLINRESISTANT STAPHYLOCOCCUS AUREUS (MRSA)
Risa Nursanty and Yunita
Department of Biology, State University of Shia Kuala
Jl. Shaykh Abdur Rauf No. 3 Darussalam, Banda Aceh 23111
Phone: +62 0651-742821, Fax: +62 0651-7552291,
email: nursanty01@yahoo.co.id
Abstract. Including use of antibiotics misuse and overuse has aided natural bacterial evolution by helping the microbes resistant
Become Reviews such as methicillin-resistant Staphylococcus aureus (MRSA). The MRSA resistant in disease treatment with
commonly used antibiotics needs new drug to treat Patients. Traditional herb can be an alternative treatment Reviews such as
cashew (Anacardium occidentale L.). Antibacterial activities of methanol extracts of the stem of cashew with concentration
10%, 20% and 30% Showed zone of inhibition between 17 -20 mm.

I. INTRODUCTION
The last few years found
some of Staphylococcus aureus that has
resistant to the antibiotic methicillin and
-lactam class of antibiotics such as,
penicillins, cephalosporins, Monobactam
and
carbapenem. The S. aureus group
known as methicillin-resistant
Staphylococcus aureus (MRSA). Infections
due to MRSA has spread to
fast and is found in almost all
State [1,2]. Antibiotic resistance is
experienced
S. aureus causes for
healing requires the use of
antibiotics and special treatment, so that
will cost more expensive and
current can feared accidental poisoning
treatment. This condition causes
the need for special handling of against
infection
MRSA [2].
Considering it becomes important

to look for a natural alternative to antibiotics

and inexpensive. One is the use of
medicinal plant materials that have been
proven efficacious and widely used by
society. Cashew
(Anacardium occidentale L) is one
one among plants that have the potential
as a traditional medicine. Study [3] seed
cashew stem, the leaves are young and
3. Procedures Research
a. Extraction
The leaves and stems of plants guava
cashew weighed as much as 50 grams later
in cashew nut oil can be used to
treat various diseases. The Results
study [4] showed that the extract
methanol leaves and stems of A. occidentale
can
inhibit the growth of S. aureus ATCC
29 213.
It was known that the compound
anacardid acids contained by oil
cashews able to inhibit
MRSA bacteria growth as

reported by [5]. So in this study

want to know the possibility of potential
owned by part cashew
such as leaves and stems in
inhibit the growth of MRSA bacteria.
II. METHODOLOGY
1. The research
Extract research and testing
antibacterial against S aureus bacteria
(MRSA)
The Microbiology Laboratory of the
Department of Biology

Faculty, University of Shia Kuala Banda

Aceh.
2. Materials
Leaf and stem nut
comes from the region of Aceh Besar.
MRSA bacteria come from the Laboratory
Microbiology General Hospital Zainal
Abidin (RSUZA) Banda Aceh.
maceration with n-hexane 500 ml.
Then covered with aluminum foil
foil and stored for 24 hours. Then
maceration results filtered by using
filter paper to obtain the filtrate

THE POTENT OF METHANOL EXTRACTS OF CASHEW (Anacardium occidentale L.) AGAINST

METHICILLIN-RESISTANT Staphylococcus aureus (MRSA)
(Risa Nursanty dan Yunita)

Furthermore, the filtrate was concentrated

using

methanol extract was evaporated to obtain

cashew leaves and stems. Extracts were

tool rotary evaporator. Residues of

workmanship

obtained used as a stock solution with

maceration n-hexane macerated back

concentration of 100%, and then diluted

using methanol to obtain

to obtain a concentration of 10%, 20% and

methanol filtrate. The filtrate is then

30%. Furthermore, each concentration

The extract in bioassay against bacteria S.

aureus MRSA.

MRSA bacterial colonies was 24 hours

b. Making the bacteria suspension

Methicillin-

taken by needle inoculation ago

resistant Staphylococcus aureus (MRSA)

resuspended into sterile distilled water with

by applying the bacterial colonies on the

wall
test tube. Th stirred with a needle
inokulasii until mixed colonies with
distilled water until homogeneous. Further
suspension

a few moments to condense. In the media

densely distributed as much as 0.1 bacterial
suspension
ml which has been adapted to the standard
0.5
Mc Farland (MCF) by using
sterile spreader rod until the bacterial
suspension
evenly across the surface of the media.
Media

20 mL with concentration (A: 30%, B: 20%,

C: 10%) in the different areas. Disc
which contains a positive control (30 mg
linezolid)
placed on other media to control
negative (solvent) in different areas (D
and E). Furthermore, the media were
incubated at
37 C for 24 hours and observed growth
bacteria inhibition zone on each
area. If the inhibition zone has not been
established
or have not looked media incubated and
observed back. Regional barriers that seem
measured with a ruler in units
millimeter [6].
II. RESULTS AND DISCUSSION
a few moments to condense. In the media
densely distributed as much as 0.1 bacterial
suspension
ml which has been adapted to the standard
0.5
Mc Farland (MCF) by using
sterile spreader rod until the bacterial
suspension
evenly across the surface of the media.
Media

MHA first divided into 3 parts

MHA first divided into 3 parts

each placed discs containing

each placed discs containing

methanol extract of cashew as

methanol extract of cashew as

20 mL with concentration (A: 30%, B: 20%,

C: 10%) in the different areas. Disc
which contains a positive control (30 mg
linezolid)
placed on other media to control
negative (solvent) in different areas (D
and E). Furthermore, the media were
incubated at
37 C for 24 hours and observed growth
bacteria inhibition zone on each
area. If the inhibition zone has not been
established
or have not looked media incubated and
observed back. Regional barriers that seem
measured with a ruler in units
millimeter [6].
II. RESULTS AND DISCUSSION
Methanol extract of the leaves and stems of
plants
cashew obtained from the extraction
each further tested to
Methicillin-resistant Staphylococcus
bacteria
aureus (MRSA). More test results
Antibacterial presented in Figure 1 below,

Methanol extract of the leaves and stems of

plants
cashew obtained from the extraction
each further tested to

Methicillin-resistant Staphylococcus
bacteria
aureus (MRSA). More test results
Antibacterial presented in Figure 1 below,
synchronized with a standard 0.5 Mc
Farland
(MCF) at a concentration of 108 CFU / ml
of bacteria.
c. Antibacterial test against MRSA bacteria.
Testing was conducted using KirbyBauer uses paper discs. Media
MHA sterilized poured into each