Documenti di Didattica
Documenti di Professioni
Documenti di Cultura
Reversed-Phase
C18 Columns (ODS)
14
15
YMC-Triart C18
1623
YMC-UltraHT series
YMC-UltraHT series
YMC-UltraHT Pro C18
YMC-UltraHT Hydrosphere C18
24,25
26
27
Pro series
Features of Pro series ODS columns
YMC-Pack Pro C18
Hydrosphere C18
YMC-Pack Pro C18 RS
2831
3235
3639
4043
ODS-A
ODS-AM
ODS-AQ
ODS-AL
44,45
46,47
48,49
50,51
,
J sphere ODS series
ODS-H80M80L80
52,53
YMC-Pack PolymerC18
54,55
13
01.indd 13
11.1.28 10:39:03 AM
Products
Hybrid silica
ODS
YMC-Triart C18
Silica
YMC-UltraHT series
YMC-Pack Pro C18
Hydrosphere C18
YMC-Pack Pro C18 RS
YMC-Pack ODS series
J
sphere ODS series
Polymer
YMC-Pack PolymerC18
ODS
Pore Particle
Usable
size
size
Silanol pH range
m C% treatment
YMC-Triart C18
120
20
Yes
5
120
16
Yes
Pro C18
120
Pro C18 RS
YMC-Pack ODS series
80
120
ODS-A
200
300
ODS-AM
ODS-AQ
120
120
200
ODS-AL
J'sphere ODS series ODS-H80
ODS-M80
ODS-L80
YMC-Pack PolymerC18
120
80
80
80
2
3
5
3
5
3
5
3
5
10
5
10
5
10
3
5
3
5
10
5
10
5
4
4
4
6
10
Pages
UltraHT series
Pro C18
Characteristics
2.0 8.0
12
26
Superior separation of
hydrophilic compounds
Reduced analysis time while
maintaining resolution
27
Yes
12
Yes
22
Yes
16
17
12
Yes
7
17
14
Yes
Yes
10
17
22
14
9
No
Yes
Yes
Yes
2.0 7.5
44,45
44,45
46,47
Superior separation of
hydrophilic compounds
48,49
50,51
52,53
54,55
14
01.indd 14
11.1.28 10:39:04 AM
Low molecular
weight compounds
High molecular
weight compounds
Hydrophobicity diers
greatly among solutes
Dicult to separate
using standard ODS
Hydrosphere C18
YMC-Pack Pro C18 RS
YMC-Pack ODS-AL
Jsphere ODS-H80
Jsphere ODS-L80
YMC-Pack PolymerC18
Basic compounds
YMC-Triart C18
YMC-Pack Pro C18
Hydrosphere C18
YMC-Pack Pro C18 RS
YMC-Pack ODS-AM
YMC-Pack PolymerC18
Coordination compounds
YMC-Triart C18
YMC-Pack Pro C18
Hydrosphere C18
YMC-Pack Pro C18 RS
YMC-Pack ODS-AM
YMC-Pack PolymerC18
Hydrosphere C18
YMC-Pack ODS-AQ
Jsphere ODS-L80
ODS
YMC-Triart C18
YMC-Pack Pro C18
YMC-Pack ODS-A
Jsphere ODS-M80
YMC-Triart C18
YMC-Pack Pro C18 RS
Jsphere ODS-H80
YMC-Pack PolymerC18
15
01.indd 15
11.1.28 10:39:05 AM
YMC-Triart C18
YMC-Triart C18 is a hybrid silica based ODS column emphasizing durability, low operating pressure, and excellent performance.
YMC-Triart C18 has been developed and is manufactured using state-of-the-art technologies that define materials, granulation
and surface modification.
U
sable over a wide pH range makes the column excellent for
method development.
A
bility to use on both older and newer instrumentation.
Features
R
ugged, first choice option for analysis of acids, bases and
neutral compounds.
ODS
Specifications
Base
Carbon %
Stationary
Bonding
polymeric type
Particle
2 m, 3 m, 5 m
Endcapping
multi-stage endcapping
Pore
120
pH range
pH 1 12
20%
inorganic-silane layer
Particle
synthesis by
Microreaction
Versatile
hybrid silica
material
Multi-stage
endcapping
Image structure
for hybrid-silica gel
organic-silane layer
16
01.indd 16
11.1.28 10:39:08 AM
Triart C18
4500
4000
3000
2500
2000
1500
1000
500
0
50
100
150
200
Pore size ()
250
300
350
ODS
d Vp/d logD
3500
Endcapping methods employed for production of conventional products typically employ a single endcapping compound in one
chemical step to "neutralize" highly reactive silanols. Unfortunately, highly reactive silanols are easily hydrolyzed and contribute to
poor durability. Additionally, low reactive silanols endcap stubbornly, producing a surface modification that is often non-reproducible,
and leading to peak tailing. YMC-Triart C18 solves this problem by using an innovative, newly developed "measured multi-stage/multicompound surface modification" technology which results in the thorough endcapping of all silanols that are accessible to analytes.
Triart C18
60
Before
EC
STEP
STEP
40
3
20
0
0.0
Silanol group
60
40
10.0
15.0
min
mAU
STEP
Q090928C
5.0
Endcapping
by traditional method
Peak tailing for basic compounds
3
1
20
2
0
0.0
Q090928E
5.0
10.0
15.0
min
Column
5 m, 1503.0 mmI.D.
Eluent
20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (65/35)
Flow rate 0.425 mL/min
Temperature 40
Detection UV at 235 nm
Sample
1. Chlorpheniramine2. Dextromethorphan
3. Propyl p-hydroxybenzoate
17
01.indd 17
11.1.28 10:39:14 AM
YMC-Triart C18
Basic compounds
2
Lot. E
Lot. E
Lot. D
Lot. D
Lot. C
Lot. C
Lot. B
Lot. B
Lot. A
Lot. A
0.0
2.5
5.0
7.5
10.0
12.5
15.0
17.5
0.0
min
2. Dextromethorphan
12.0
14.0 min
Column
5 m, 1503.0 mmI.D.
Eluent
acetonitrile/0.1% H3PO4 (40/60)
Flow rate 0.425 mL/min
Temperature 40
Detection UV at 254 nm
15000
2.0
10000
1.5
Tailing factor
5000
1.0
10
20
30
40
50
0.5
18
16
14
12
10
8
6
4
2
20
30
40
50
60
70
80
90
20
10
Column
5 m, 1504.6 mmI.D.
Eluent
acetonitrile/water (60/40)
Flow rate 1.0 mL/min
Temperature ambient
Sample
butyl benzoate
100
Column
5 m, 1504.6 mmI.D.
Eluent
acetonitrile/water or methanol/water
Flow rate 1.0 mL/min
Temperature 25
10.0
2.5
Column number
8.0
6.0
Rigid control of theoretical plate number (N) and tailing factor (Tf) are exhibited for each YMC-Triart C18 packed
column.
20000
4.0
1. Hinokitiol
2. Methyl benzoate
Column
5 m, 1503.0 mmI.D.
Eluent
20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (65/35)
Flow rate 0.425 mL/min
Temperature 40
Detection UV at 235 nm
Packed Column
2.0
J091201X
J091201Y
1. Chlorpheniramine
3. Propyl p-hydroxybenzoate
Tailing factor
ODS
Coordination compounds
0.180
0.160
Develosil C30-UG
Hydrosphere C18
0.140
Atlantis T3
0.120
Standard ODS
Pro C18
0.100
Triart C18
0.080
TSKgel ODS-100S
Cadenza CD-C18
L-column2 ODS
Develosil ODS-SR
Pro C18 RS
0.060
0.040
2.00
4.00
6.00
8.00
10.00
Hydrophobicity k (amylbenzene)
12.00
14.00
(S090805A)
18
01.indd 18
11.1.28 10:39:17 AM
120%
YMC-Triart C18
100%
80%
60%
40%
20%
0%
10
20
30
40
80%
60%
40%
20%
0%
50
YMC-Triart C18
100%
50
100
Time (hr)
150
200
250
ODS
120%
300
Time (hr)
Column
5 m, 1504.6 mmI.D.
Eluent
50 mM K2HPO4-K3PO4 (pH 11.5)/methanol (90/10)
Flow rate 1.0 mL/min
Temperature 40
Sample
benzyl alcohol
Column
5 m, 1504.6 mmI.D.
Eluent
50 mM triethylamine (pH 11.5)/methanol (90/10)
Flow rate 1.0 mL/min
Temperature 40
Sample
benzyl alcohol
pH 6.9, 70
pH 1, 70
120%
120%
YMC-Triart C18
100%
100%
YMC-Triart C18
80%
60%
40%
40%
20%
0%
0%
100
200
300
400
500
600
700
60%
20%
20
40
60
80
100
120
Time (hr)
Time (hr)
Column
5 m, 502.0 mmI.D.
Eluent
20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (90/10)
Flow rate 0.2 mL/min
Temperature 70
Sample
phenol
YMC-Triart C18 shows great chemical stability provided by newly developed hybrid silica. Even in high pH or high temperature
conditions, the lifetime of YMC-Triart C18 is more than 10 times greater than conventional ODS columns.
1.2E+09
Triart C18
No column
8.0E+08
Column
Eluent
4.0E+08
0.0E+00
10
12 min
5 m, 502.0 mmI.D.
A) water/formic acid (100/0.1)
B) acetonitrile/formic acid (100/0.1)
5% B (0-1 min), 5-100% B (1-5 min), 100% B (5-10 min),
100-5% B (10-10.1 min), 5% B (10.1-12.5 min)
Flow rate 0.4 mL/min
Temperature 40
Detection ESI positive, TIC (Mass Range: 50-1000)
Bleeding of stationary phase can often contribute to high background signals when employing MS and other hyphenated detection
techniques. In the example cited above, use of a 502.0 mmI.D. YMC-Triart C18 column shows no contribution to the signal observed
by the MS positive total ion current (TIC) trace. Such performance allows for low limits of detection (high S/N ratio) for MS and other
detection techniques. The inherent hybrid particle strength is also superior to conventional silica materials for detection techniques
that are sensitive to particle degradation.
19
01.indd 19
11.1.28 10:39:21 AM
YMC-Triart C18
5 m, 1503.0 mmI.D.
1.
Cl
CH3
2.
CH3
H3C
3
N
ODS
N
H
Q090928C
CH3
Chlorpheniramine
1
2
5 m, 1504.6 mmI.D.
3.
O
H3C
O
Q090821C
Propyl p-hydroxybenzoate
Column
Eluent
Flow rate
3 m, 1503.0 mmI.D.
J091111K
1 2
Dextromethorphan
OH
5 m, 1504.6 mmI.D.
Q090123F
0.0
2.5
5.0
7.5
10.0
12.5
15.0
17.5
min
Hinokitiol
CH3
1.
CH3
2.
O
CH3
YMC-Triart C18
5 m, 1503.0 mmI.D.
OH
O
Q090925G
2
1
3 m, 1503.0 mmI.D.
J091110B
0.0
2.0
4.0
Q090120A
6.0
8.0
10.0
12.0
14.0 min
5 m, 1503.0 mmI.D.
Q090605C
5 m, 1504.6 mmI.D.
Q090717A
1.5
2.0
1. Formic acid
2. Acetic acid
3. Propionic acid
1.0
Column
Eluent
Flow rate
Column
Eluent
Flow rate
0.5
Methyl benzoate
Organic acid
0.0
Hinokitiol
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
7.5 min
20
01.indd 20
11.1.28 10:39:24 AM
10 mM CH3COONH4/organic solvent
methanol
acetonitrile
acetonitrile
YMC-Triart C18
502.0 mmI.D.
Tf 0.97
Tf 1.11
Tf 1.21
502.1 mmI.D.
Tf 1.56
F090727H07
Tf 1.03
Hybrid-silica
based C18
Brand I8
Tf 1.14
502.1 mmI.D.
10.0
H3C
N
11.0
12.0
Tf 1.78
F090731G07
F090731F07
min
8.0
9.0
10.0
min
6.0
7.0
8.0
min
Column
Eluent
CH3
8.0
F090722G07
Tf 1.22
F090731I07
7.0
Tf 1.88
F090722F07
Tf 1.88
6.0
F090714G07
Tf 1.13
F090722I07
min
Tf 2.21
F090714F07
Tf 3.87
F090731H07
502.1 mmI.D.
F090803G07
Tf 1.05
F090717I07
F090722H07
Tf 1.43
F090727F07
Tf 2.83
F090717H07
F090817G07
Tf 1.13
F090727I07
Tf 0.93
502.0 mmI.D.
Tf 1.16
F090818F07
ODS
Tf 1.03
F090818I07
F090818H07
Cl
Clemastine
Clemastine is a well known basic compound which can easily tail on conventional ODS columns. YMC-Triart C18 can separate
clemastine sharply using many buffer/solvent combinations.
injection volume
acetonitrile
1.0 L
1.5 L
2.0 L
3.0 L
YMC-Triart C18
Column
Eluent
2.
OCH2CHCH2NHCH(CH3)2
OH
H3CO
H3CO
CN
CH3
C (CH2)3NCH2CH2
OCH3
OCH3
CH(CH3)2
1.5
2.0
2.5
min
YMC-Triart C18 can tolerate larger injection volumes of samples containing strong solvents (e.g., acetonitrile) while allowing for
better peak shape than traditional columns. This can be important for difficult to solubilize samples that need higher concentrations of
organic solvent for solubilization prior to analysis.
21
01.indd 21
11.1.28 10:39:27 AM
YMC-Triart C18
25
20
ODS
mAU
20
Eluent optimization
Insufficient
separation
15
10
R091126J
Baseline separation,
But long analysis time
15
10
25
10
12
14 min
R091127C
10
15
20
25 min
Downsizing
1.
2.
N+
N+
mAU
2Br
N+ CH3
2Cl
H3C N+
25
20
Paraquat
Diquat
15
10
Eluent
5
0
R091201B
Application
min
Q091110E
Anthocyanin
1. Delphinidin-3-O-galactoside
2. Delphinidin-3-O-glucoside
3. Cyanidin-3-O-galactoside
4. Delphinidin-3-O-arabinoside
5. Cyanidin-3-O-glucoside
6. Petunidin-3-O-galactoside
7. Cyanidin-3-O-arabinoside
8. Petunidin-3-O-glucoside
9. Peonidin-3-O-galactoside
10. Petunidin-3-O-arabinoside
11. Delphinidin
12. Peonidin-3-O-glucoside
13. Malvidin-3-O-galactoside
14. Peonidin-3-O-arabinoside
15. Malvidin-3-O-glucoside
16. Malvidin-3-O-arabinoside
17. Cyanidin
18. Petunidin
19. Peonidin
20. Malvidin
mAU
100
80
4
5
60
3
8
7
40
15
6
16
17
12
20
10
11 13
18 20
19
14
0
0
10
15
20
25
30
35
40
min
Column
22
01.indd 22
11.1.28 10:39:38 AM
Application
R091009C
Peptide
mAU
1. Oxytocin
2. Met-Enkephalin
3. Leu-Enkephalin
4. Neurotensin
5. -Endorphin
6. -Endorphin
100
1
75
50
Column
5
25
10
15
25 min
20
Application
S100121A
1.
+
forM+H
H3C
N
N
Cl
2.
N
N
H3C
S
H3C
N
Cl
Cl
Column
1
0
0
10
12
14
Time [min]
ODS
(MW 1,007)
(MW 574)
(MW 556)
(MW 1,673)
(MW 1,859)
(MW 3,465)
Ordering information
Analytical columns
Particle size
m
S-3
S-5
Semi-preparative columns
inner diameterlengthmm
120
120
2.0 20
2.0 30
2.0 50
2.0 75
2.0100
2.0150
3.0 50
3.0100
3.0150
4.6 35
4.6 50
4.6 75
4.6100
4.6150
2.0 20
2.0 30
2.0 50
2.0 75
2.0100
2.0150
3.0 50
3.0 75
3.0100
3.0150
4.6 50
4.6 75
4.6100
4.6150
4.6250
Product number
TA12S03-0202WT
TA12S03-0302WT
TA12S03-0502WT
TA12S03-L502WT
TA12S03-1002WT
TA12S03-1502WT
TA12S03-0503WT
TA12S03-1003WT
TA12S03-1503WT
TA12S03-H546WT
TA12S03-0546WT
TA12S03-L546WT
TA12S03-1046WT
TA12S03-1546WT
TA12S05-0202WT
TA12S05-0302WT
TA12S05-0502WT
TA12S05-L502WT
TA12S05-1002WT
TA12S05-1502WT
TA12S05-0503WT
TA12S05-L503WT
TA12S05-1003WT
TA12S05-1503WT
TA12S05-0546WT
TA12S05-L546WT
TA12S05-1046WT
TA12S05-1546WT
TA12S05-2546WT
Particle size
m
S-5
inner diameterlengthmm
120
10150
10250
20100
20150
20250
Product number
TA12S05-1510WT
TA12S05-2510WT
TA12S05-1020WT
TA12S05-1520WT
TA12S05-2520WT
TA12S05-1020WX
TA12S05-0530WX
TA12S05-L530WX
TA12S05-1030WX
Guard cartridge columns (with inner diameter 2.0 mm: 2/pack, 4.0 mm: 3/pack)
TA12S03-0102CC
2.010
S-3
120
S-5
120
4.023
2.010
4.023
TA12S03-G304CC
TA12S05-0102CC
TA12S05-G304CC
A cartridge holder will need to be purchased separately before using this product for the first time.
Cartridge holder
Semi-micro guard cartridge holder
(inner diameter: 1.0, 1.5, or 2.0 mm)
Cartridge holder
(inner diameter: 4.0 mm)
Guard columns
S-5
120
1030
2050
XPCHSMW
XPCHW
TA12S05-0310WTG
TA12S05-0520WTG
23
01.indd 23
11.1.28 10:39:40 AM
Ultra-Fast LC column
YMC-UltraHT
ODS
conditions.
Column pressure
0.020
80
Optimum ow rate
for 5 m
0.015
YMC 3 m
0.010
Brand L1 1.8 m
YMC 2 m
0.005
70
YMC 5 m
HETP (mm/plates)
Brand I7 1.7 m
Brand I7 1.7 m
Brand L1 1.8 m
60
50
40
YMC 2 m
applicable
pressure range
for conventional LC
30
YMC 3 m
20
YMC 5 m
10
0.000
10
11
10
11
Column
: 502.0 or 2.1 mmI.D.
Eluent
: acetonitrile/water (60/40)
Temperature : 25
Sample
: Butyl benzoate
YMC-UltraHT series
Pro C18
Particle Size (m)
Pore Size ()
Hydrosphere C18
120
120
330
330
Carbon Content
16%
12%
Monomeric
Monomeric
Bonding Type
Available pH Range
Characteristics
2-8
2-8
Standard type
24
01.indd 24
11.1.28 10:39:43 AM
1.0 mL/min
5 L injection
8.5 MPa
Ultra-fast LC methodYMC-UltraHT
C18
502.0 mmI.D. 2 m
Flavonoids
1. Myricetin
2. Quercetin
3. Apigenin
4. Kaempferol
5. Baicalein
6. Chrysin
7. Acacetin
0.6 mL/min
1 L injection
30 MPa
Eluent
: acetonitrile/water/formic acid
(35/65/0.1)
Temperature : 40
Detection : UV at 260 nm
ODS
Triplear
line city
Velo
This example shows the method transfer from conventional HPLC method to Ultra-fast LC method using a YMC-UltraHT column. Since
the column efficiency is not decreased even when the flow rate is 3 times as fast as the conventional condition, it can be shorten
analysis time by 90% without losing the separation characteristics.
80
60
40
20
0
502 mmI.D.
N(1) = 6300
N(2) = 6300
N(3) = 7500
N(4) = 7800
Rs(1, 2) = 2.21
mAU
1
100
50
3 min
N(1) = 3500
N(2) = 3800
N(3) = 6100
N(4) = 6800
Rs(1, 2) = 1.61
mAU
150
3 min
12
100
50
0
(N070606E)
N(1) = 3700
N(2) = 4100
N(3) = 6100
N(4) = 6900
Rs(1, 2) = 1.61
(N070608E)
20
3 min
40
0
1
150
60
(N070606B)
mAU
N(1) = 1400
N(2) = 1500
N(3) = 3600
4
N(4) = 4500
Rs(1, 2) = 0.90
(N070608B)
3 min
Cephalosporin antibiotics
1. Cephalexin
2. Cefaclor
3. Cephaloglycin
4. Cephaloridine
Column
YMC-UltraHT Pro C18
Eluent
acetonitrile/20 mM KH2PO4
(10/90)
Temperature 37
Detection UV at 260 nm
25
01.indd 25
11.1.28 10:39:45 AM
Analytical columns
Pore size120
Carbon content16
Usable pH range2.0 8.0
YMC-UltraHT
C18
502.0 mmI.D 2 m
1
40
N(5) 106,000/m
Tf(5) 1.07
30 MPa
20
mV
60
sub-2 m ODS
2
20
N(5) 89,000/m
Tf(5) 3.99
49 MPa
40
mV
60
40
0.4
0.8
1.2
1.6
2.0
[min]
N(5) 78,000/m
Tf(5) 2.35
37 MPa
1.2
1.6
20
0
0.0
sub-2 m ODS
0.0
0.4
0.8
1.2
1.6
Alkaloids
1. Scopolamine
2. Atropine
3. Cinchonine
4. Quinine
5. Dihydroquinine
2.0
[min]
0.0
0.4
0.8
2.0
[min]
Column
: 502.0 or 2.1 mmI.D.
Eluent
:2
0 mM CH3COOH-CH3COONH4 (pH 4.9)/
acetonitrile (80/20)
Flow rate
: 0.6 mL/min
Temperature : 40
Detection : UV at 220 nm
Peptide mapping
mV
YMC-Pack
C18
1502.0 mmI.D. 5 m
80
40
(06082204.D)
15
mV
YMC-UltraHT
C18
502.0 mmI.D. 2 m
200
45
30
100
min
*undigested
Cytochrome c
N060811I
10
15
min
Ordering information
Analytical columns
Particle size
m
S-2
inner diameterlengthmm
120
2.0 30
2.0 50
2.0 75
2.0100
3.0 50
3.0 75
3.0100
Product number
AS12S02-0302WT
AS12S02-0502WT
AS12S02-L502WT
AS12S02-1002WT
AS12S02-0503WT
AS12S02-L503WT
AS12S02-1003WT
26
01.indd 26
11.1.28 10:40:11 AM
Analytical columns
Pore size120
Carbon content12
Usable pH range2.0 8.0
Rs(10,11)=2.38
2
4
11
12
10
7 8
(N060509E)
10 min
ODS
Rs(10,11)=2.91
Rs(10,11)=2.91
Rs(7,8)=2.46
3
3
1
1 2
2
9
9
5
4 5
4
6
6
12
12
10
10 11
11
7
7 8
8
(N070130I)
(N070130I)
0
0
2
2
4
4
6
6
Soy isoflavones
10
10 min
min
8
8
1
1 2
2
Rs(7,8)=1.75
5
4
5
4
6
6
7 8
7 8
Rs(10,11)=2.64
Rs(10,11)=2.64
9
9
10
10
12
12
11
11
1. Daidzin
2. Glycitin
3. Genistin
4. 6"-O-Malonyldaidzin
5. 6"-O-Malonylglycitin
6. 6"-O-Acetyldaidzin
7. 6"-O-Acetylglycitin
8. 6"-O-Malonylgenistin
9. Daidzein
10. Glycitein
11. 6"-O-Acetylgenistin
12. Genistein
MPa
(N070130L)
0
0
(N070130L)
1
1
2
2
3
3
3.3 min
3.3 min
Eluent
A) water/acetic acid (100/3)
B) acetonitrile/acetic acid (100/3)
Temperature 35
Detection UV at 254 nm
Ordering information
Analytical columns
Particle size
m
S-2
inner diameterlengthmm
120
2.0 30
2.0 50
2.0 75
2.0100
3.0 50
3.0 75
3.0100
Product number
HS12S02-0302WT
HS12S02-0502WT
HS12S02-L502WT
HS12S02-1002WT
HS12S02-0503WT
HS12S02-L503WT
HS12S02-1003WT
27
01.indd 27
11.1.28 10:40:30 AM
stable quality all over the world to fulfill the needs of the
characteristics.
ODS
Hydrosphere C18
YMC-Pack
YMC-Pack
C18 is a high
C18 RS is a high
natural products.
Pro C18
Hydrosphere C18
Pro C18 RS
2 , 3 , 5 , 10
2,3,5
3,5
120
120
80
330
330
510
Carbon Content
16%
12%
22%
monomeric
monomeric
polymeric
2-8
2-8
1 - 10
Standard type
High durability
Bonding type
Available pH Range
Characteristics
28
01.indd 28
11.1.28 10:40:32 AM
C18
Hydrosphere C18
3
C18 RS
1
5
2
2
YMC-Pack
3
4
5
4
J020805B
1. Uracil
2. n-Butylbenzene
3. o-Terphenyl
4. n-Amylbenzene
5. Triphenylene
10
15
20 min
J020805D
10
15
20 min
J020805E
10
15
20
25 min
ODS
YMC-Pack
0.160
Develosil C30-UG
Hydrosphere C18
0.140
Atlantis T3
0.120
Hydrophobicity: intermediate
Hydrogen-bonding capacity: middle
Pro C18
0.100
0.080
TSKgel ODS-100S
Cadenza CD-C18
L-column2 ODS
Hydrophobicity: high
Hydrogen-bonding capacity: low
Develosil ODS-SR
Pro C18 RS
0.060
0.040
2.00
4.00
6.00
8.00
10.00
Hydrophobicity k (amylbenzene)
12.00
14.00
(S090805A)
29
01.indd 29
11.1.28 10:40:35 AM
Comparison of separation behavior on the three kinds of ODS columns in the Pro series
Parabenzoates
C18
ODS
1
1
1.
OH
2
2
COOCH3
Methyl p-hydroxybenzoate
2.
OH
34
3 4
COOCH2CH3
Ethyl p-hydroxybenzoate
34
3.
CH3
OH
COOCH
CH3
56
Isopropyl p-hydroxybenzoate
5 6
4.
OH
C18 RS
Hydrosphere C18
COO(CH2)2CH3
Propyl p-hydroxybenzoate
5.
OH
20 min
10
J020827D
J020827C
10
20 min
J020827E
10
20
30 min
CH3
COOCH2CH
CH3
Isobutyl p-hydroxybenzoate
6.
OH
Column
1504.6 mmI.D.
Eluent
methanol/water (55/45)
Flow rate 1.0 mL/min
Temperature 30
Detection UV at 270 nm
COO(CH2)3CH3
Butyl p-hydroxybenzoate
Flavonoid glycosides
2
1
C18
J020828A
HO
OH
J020828F
2
3
4
C18 RS
J020828C
OH
Hydrosphere C18
HO
10
15
min
1. Rutin
2. Isoquercitrin
3. Quercitrin
4. Quercetin
OR
O
R
R
R
R
= rutinose
= glucose
= rhamnose
=H
Hydrogen-bonding
interactions significantly
contribute to the retention
of hydrophilic compounds,
including glycosides.
Therefore, the retention is
longer in the Hydrosphere
C18, which has strong
hydrogen-bonding capacity,
than in Pro C18 RS, which
has highly hydrophobic
packing materials. To
separate such hydrophilic
compounds, the Hydrosphere
C18 is suitable for
precise separation of
multicomponent samples,
while Pro C18 RS and
Pro C18 are suitable for
rapid separation of simple
components.
Column
1504.6 mmI.D.
Eluent
acetonitrile/10 mM H3PO4 (25/75)
Flow rate 1.0 mL/min
Temperature 40
Detection UV at 260 nm
30
01.indd 30
11.1.28 10:40:39 AM
Terphenyl isomers
C18
C18 RS
Hydrosphere C18
Brand C1
2,3
1.
1 3
1
o-Terphenyl
1
1
2.
m-Terphenyl
3.
0
J020719J
10
20 min
J020722A
10
20 min
J020719E
10
20 min
J020719K
10
ODS
20 min
p-Terphenyl
Column
1504.6 mmI.D.
Eluent
methanol/water
(85/15)
Flow rate 1.0 mL/min
Temperature 37
Detection UV at 254 nm
Saikosaponins
Peaks 2 and 3, which are not easily separated by other
ODS, can be completely separated by Hydrosphere C18. It
is thought that hydrogen-bonding interaction of hydrophilic
functional groups in sugar chain and terpene skeleton as
well as hydrophobic interaction has great contribution to the
separation of saikosaponins.
Hydrosphere C18 is the most appropriate in the separation of
this kind of class of compound.
3
2
C18
F030222B
Hydrosphere C18
23
1.
3.
CH2OH
OH
OH
F030222A
1
2,3
glucose 6 glucose
fucose
rhamnose
C18 RS
Saikosaponin c
2.
H
OH
OH
O
2,3
fucose
4
10
20
30
CH2OH
glucose
Saikosaponin a
F030224E
4.
Brand B2
CH2OH
glucose
Saikosaponin b2
F030222C
40 min
fucose
CH2OH
glucose
Saikosaponin d
Column
1504.6 mmI.D.
Eluent
acetonitrile/water (38/62)
Flow rate 1.0 mL/min
Temperature 37
Detection UV at 210 nm
31
01.indd 31
11.1.28 10:40:44 AM
Analytical columns
Excellent reproducibility
Utilizes highly pure silica gel base
T
wo types of test reports are included
endcapping technology
Pore size120
Carbon content16
Usable pH range2.0 8.0
Two test reports are included with each product; a Column Test Report,
indicating the column performance, and a Packing Material Test
Report, detailing strictly controlled physical properties and separation
characteristics of the packing material.
23
1
2.
HCI
N
CH2CH2CH2NHCH3
Desipramine hydrochloride
Acenaphthene(I.S.)
3.
4.
HCI
HCI
N
CH2CH2CH2N
CH3
CHCH2CH2N
CH3
Imipramine hydrochloride
10
CH3
CH3
Amitriptyline hydrochloride
20 min
A961015G
Column
YMC-Pack Pro C18 (5 m, 120 )
1504.6 mmI.D.
Eluent
20 mM KH2PO4-K2HPO4 (pH6.9)/
methanol (25/75)
Flow rate 1.0 mL/min
Temperature 37
Detection UV at 254 nm
Basic peptides
C18
1. Bradykinin
2. Dynorphin A (Human,1-13)
3. Eledoisin related peptide
4. Neurotensin
5. Bombesin
6. Substance P
ODS-A
4
6
2
3
2
3
10
15
20
min
A990123B
10
15
20
min
A990121A
Neutral peptides
C18
2
3
ODS-A
5
4
2
6
Column
YMC-Pack Pro C18 and ODS-A (5 m, 120 )
754.6 mmI.D.
Eluent
A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
20-40 B (0-20 min,linear)
Flow rate 1.0 mL/min
Temperature 37
Detection UV at 220 nm
5
4
A990121B
10
15
20 min
A990119B
10
15
20 min
1. Oxytocin
2. Met-Enkephalin
3. Leu-Enkephalin
4. Angiotensin l
5. -Mating factor
6. Insulin
32
01.indd 32
11.1.28 10:40:48 AM
Test B
1. Caffeine
2. Pyridine
3. Phenol
Lot C
Lot C
Lot B
Lot B
Lot A
Lot A
10
15
ODS
1. Uracil
2. 4-n-Butoxybenzoic acid
3. Ethylbenzene
4. n-Propylbenzene
min
min
column-to-column
1504.6 mml.D. theoretical plate number
24000
21000
18000
15000
12000
9000
6000
3000
20
40
60
80
100
Column No.
33
01.indd 33
11.1.28 10:40:51 AM
Analytical columns
Application
3
4 5
C18
5
3
Brand I4
1
4, 5
Brand L1
1
2
2
6
0
10
F040119B
15
20 min
10
F030130C
15
20 min
F040130E
10
15
20 min
2
CHCOOH
CHCOOH
CH3 O
NHCCH2 N(CH2 CH3 )2
CH3
4
H
Maleic acid
Lidocaine
6
OH
COOCH3
CH3
CH3
CH3
Chlorpheniramine
CH3
CH3 C CH2 C
CH3
CH3
Methyl p-hydroxybenzoate
Cl
Naphazoline
CH3
N
CHCH2CH2N
CH2
ODS
(OCH2CH2)2 N CH2
CH3
Cl-
Column
1504.6 mmI.D.
Eluent
A) 20 mM KH2PO4-H3PO4 (pH 2.5)
B) methanol
20-90% B (0-15 min), 90% B (15-20 min)
Flow rate 1.0 mL/min
Temperature 37
Detection UV at 260 nm
Benzethonium chloride
Application
N050620A
catechins
1
1. (-)-Epigallocatechin
2. (+)-Catechin
3. (-)-Epicatechin
4. (-)-Epigallocatechin gallate
5. (-)-Epicatechin gallate
Column
: YMC-Pack Pro C18 (5 m, 120 )
1504.6 mmI.D.
Eluent
: acetonitrile/ethyl acetate/water/TFA
(9/3/88/0.1)
Flow rate
: 1.0 mL/min
Temperature : 37
Detection : UV at 280 nm
10
20
min
Application
A970526C
antioxidants
5
1. n-Propyl gallate(PG)
2. 2,4,5-Trihydroxybutyrophenone (THBP)
3. tert-Butylhydroquinone(TBHQ)
4. Nordihydroguaiaretic acid (NDGA)
5. 4-Hydroxymethyl-2,6-di-tert-butylphenol (HMBP)
6. n-Octyl gallate(OG)
7. n-Dodecyl gallate(DG)
4
6
1
Column
YMC-Pack Pro C18 (5 m, 120 )
754.6 mm I.D.
Eluent
A) acetonitrile/methanol/5% acetic acid (20/20/60)
B) acetonitrile/methanol/5% acetic acid (45/45/10)
0-100% B (0-5 min), 100% B (5-15 min)
Flow rate 1.0 mL/min
Temperature 30
Detection UV at 280 nm
10
15
min
34
01.indd 34
11.1.28 10:40:55 AM
Ordering information
Analytical columns
Particle size
m
S-3
S-10
inner diameterlengthmm
120
120
120
1.0 35
1.0 50
1.0100
1.5 35
1.5 50
1.5100
2.0 35
2.0 50
2.0 75
2.0100
2.0150
3.0 50
3.0100
3.0150
4.6 35
4.6 50
4.6 75
4.6100
4.6150
6.0 50
6.0 75
6.0100
1.0150
1.0250
1.5150
1.5250
2.0 35
2.0 50
2.0 75
2.0100
2.0150
2.0250
3.0 50
3.0 75
3.0100
3.0150
3.0250
4.6 50
4.6 75
4.6100
4.6150
4.6250
6.0150
6.0250
4.6150
4.6250
Product number
AS12S03-H501WT
AS12S03-0501WT
AS12S03-1001WT
AS12S03-H5P5WT
AS12S03-05P5WT
AS12S03-10P5WT
AS12S03-H502WT
AS12S03-0502WT
AS12S03-L502WT
AS12S03-1002WT
AS12S03-1502WT
AS12S03-0503WT
AS12S03-1003WT
AS12S03-1503WT
AS12S03-H546WT
AS12S03-0546WT
AS12S03-L546WT
AS12S03-1046WT
AS12S03-1546WT
AS12S03-0506WT
AS12S03-L506WT
AS12S03-1006WT
AS12S05-1501WT
AS12S05-2501WT
AS12S05-15P5WT
AS12S05-25P5WT
AS12S05-H502WT
AS12S05-0502WT
AS12S05-L502WT
AS12S05-1002WT
AS12S05-1502WT
AS12S05-2502WT
AS12S05-0503WT
AS12S05-L503WT
AS12S05-1003WT
AS12S05-1503WT
AS12S05-2503WT
AS12S05-0546WT
AS12S05-L546WT
AS12S05-1046WT
AS12S05-1546WT
AS12S05-2546WT
AS12S05-1506WT
AS12S05-2506WT
AS12S11-1546WT
AS12S11-2546WT
Particle size
m
S-5
S-10
inner diameterlengthmm
120
120
10150
10250
20100
20150
20250
20100
20150
20250
Product number
AS12S05-1510WT
AS12S05-2510WT
AS12S05-1020WT
AS12S05-1520WT
AS12S05-2520WT
AS12S11-1020WT
AS12S11-1520WT
AS12S11-2520WT
See P142 for preparative columns other than those listed above.
Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)
AS12S03-0101CC
1.010
S-3
120
S-5
120
1.510
2.010
4.023
1.010
1.510
2.010
4.023
AS12S03-01P5CC
AS12S03-0102CC
AS12S03-G304CC
AS12S05-0101CC
AS12S05-01P5CC
AS12S05-0102CC
AS12S05-G304CC
ODS
S-5
Semi-preparative columns
A guard cartridge holder will need to be purchased separately before using this
product for the first time.
Cartridge holder
Semi-micro guard cartridge holder
(inner diameter: 1.0, 1.5, or 2.0 mm)
Cartridge holder
(inner diameter: 4.0 mm)
XPCHSMW
XPCHW
AS12S03-G304CCPK
AS12S05-G304CCPK
Guard columns
S-5
120
S-10
120
1030
2050
AS12S05-0310WTG
AS12S05-0520WTG
2050
AS12S11-0520WTG
When columns packed with material from different lots are needed for validation
purposes, please contact us.
35
01.indd 35
11.1.28 10:40:56 AM
Analytical columns
Hydrosphere C18
Strong retention of hydrophilic compounds
Can be used with 100% water mobile phase
Superior separation of basic compounds
Pore size120
Carbon content12
Usable pH range2.0 8.0
Excellent reproducibility
Hydrophilic ODS
Hydrosphere C18 is designed to maintain adequate hydrophilicity on
the packing surface for superior separation of hydrophilic compounds.
Hydrophilic compounds are retained with much greater affinity than
with conventional ODS columns, permitting the product to be used
ODS
Hydrosphere C18
1
1
3
3,4
A000310E
10
min
A000615A
10
Column
1504.6 mm I.D.
Eluent
20 mM NaH2PO4-H3PO4 (pH 2.8)
Flow rate 1.0 mL/min
Temperature 30
Detection UV at 210 nm
1. Lactic acid
2. Acetic acid
3. Citric acid
4. Fumaric acid
5. Succinic acid
min
Reproducibility of retention time when used with 100% water mobile phase
conventional ODS columnBrand
Hydrosphere C18
Before standing
10
20 min
S0517A
10
S0518H
After standing
S0515A
2
1
10
98
20 min
S0519A
20
min
S0518F
10
89
10
20
min
S0519C
10
20 min
71
20 min
After completion of analysis, the flow was stopped and the columns were left to stand overnight (ca. 15 hours).
1. Cytosine
2. Uracil
3. Guanine
4. Thymine
5. Adenine
Column
1504.6 mm I.D.
Eluent
20 mM KH2PO4-K2HPO4 (pH 6.9)
Flow rate 1.0 mL/min
Temperature 37
Detection UV at 254 nm
36
01.indd 36
11.1.28 10:41:01 AM
10
S000309A
20
30
40
min
C18
2
1
10
S990607B
20
30
40
min
ODS
Brand
1
2
5
6
10
F000614B
20
30
40
50
60
min
Brand
5
6
F000614C
10
20
30
40
50
1. Toluene
2. Testosterone
3. n-Propylbenzene
4. Acenaphthene
5. n-Butylbenzene
6. Amitriptyline hydrochloride
60
70
80
90
min
Column
1504.6 mm I.D.
Eluent
20 mM KH2PO4-K2HPO4 (pH 6.9)/methanol (35/65)
Flow rate 1.0 mL/min
Temperature 37
Detection UV at 254 nm
37
01.indd 37
11.1.28 10:41:04 AM
Analytical columns
Hydrosphere C18
Hydrosphere C18
2 4
2 4
ODS
Antioxidants
J010216B
10
15
min
J010215B
10
15
min
Column
504.6 mm I.D. (3 m, 120 )
Eluent
acetonitrile/methanol/5% acetic acid (35/35/30)
Flow rate 1.0 mL/min
Temperature 30
Detection UV at 280 nm
Application
F000707B
1 2
Column
Hydrosphere C18 (5 m, 120 )
1504.6 mm I.D.
Eluent
20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (99/1)
Flow rate 1.0 mL/min
Temperature 37
Detection UV at 260 nm
10
15
20
25
min
Application
A000922A
Nucleotides
5
1. 5-UTP
2. 5-UDP
3. 5-CMP
4. 5-UMP
5. 5-dCMP
6. 5-GMP
1
7
2
10
Column
Hydrosphere C18 (5 m, 120 )
1504.6 mm I.D.
Eluent
100 mM KH2PO4-K2HPO4 (pH 5.5)
Flow rate 1.0 mL/min
Temperature 30
Detection UV at 260 nm
11
10
15
7. 5-ATP
8. 5-IMP
9. 5-ADP
10. 5-TMP
11. 5-AMP
20 min
38
01.indd 38
11.1.28 10:41:07 AM
Ordering information
Analytical columns
Particle size
m
S-3
inner diameterlengthmm
120
120
1.0 35
1.0 50
1.0100
1.5 35
1.5 50
1.5100
2.0 35
2.0 50
2.0 75
2.0100
2.0150
3.0 50
3.0100
3.0150
4.6 35
4.6 50
4.6 75
4.6100
4.6150
6.0 50
6.0 75
6.0100
1.0150
1.0250
1.5150
1.5250
2.0 35
2.0 50
2.0 75
2.0100
2.0150
2.0250
3.0 50
3.0 75
3.0100
3.0150
3.0250
4.6 50
4.6 75
4.6100
4.6150
4.6250
6.0150
6.0250
Product number
HS12S03-H501WT
HS12S03-0501WT
HS12S03-1001WT
HS12S03-H5P5WT
HS12S03-05P5WT
HS12S03-10P5WT
HS12S03-H502WT
HS12S03-0502WT
HS12S03-L502WT
HS12S03-1002WT
HS12S03-1502WT
HS12S03-0503WT
HS12S03-1003WT
HS12S03-1503WT
HS12S03-H546WT
HS12S03-0546WT
HS12S03-L546WT
HS12S03-1046WT
HS12S03-1546WT
HS12S03-0506WT
HS12S03-L506WT
HS12S03-1006WT
HS12S05-1501WT
HS12S05-2501WT
HS12S05-15P5WT
HS12S05-25P5WT
HS12S05-H502WT
HS12S05-0502WT
HS12S05-L502WT
HS12S05-1002WT
HS12S05-1502WT
HS12S05-2502WT
HS12S05-0503WT
HS12S05-L503WT
HS12S05-1003WT
HS12S05-1503WT
HS12S05-2503WT
HS12S05-0546WT
HS12S05-L546WT
HS12S05-1046WT
HS12S05-1546WT
HS12S05-2546WT
HS12S05-1506WT
HS12S05-2506WT
Particle size
m
S-5
inner diameterlengthmm
120
10150
10250
20100
20150
20250
Product number
HS12S05-1510WT
HS12S05-2510WT
HS12S05-1020WT
HS12S05-1520WT
HS12S05-2520WT
Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)
HS12S03-0101CC
1.010
S-3
120
S-5
120
1.510
2.010
4.023
1.010
1.510
2.010
4.023
HS12S03-01P5CC
HS12S03-0102CC
HS12S03-G304CC
HS12S05-0101CC
HS12S05-01P5CC
HS12S05-0102CC
HS12S05-G304CC
A guard cartridge holder will need to be purchased separately before using this
product for the first time.
Cartridge holder
Semi-micro guard cartridge holder
(inner diameter: 1.0, 1.5, or 2.0 mm)
Cartridge holder
(inner diameter: 4.0 mm)
ODS
S-5
Semi-preparative columns
XPCHSMW
XPCHW
HS12S03-G304CCPK
HS12S05-G304CCPK
Guard columns
S-5
120
1030
2050
HS12S05-0310WTG
HS12S05-0520WTG
39
01.indd 39
11.1.28 10:41:08 AM
Analytical columns
Excellent reproducibility
Utilizes highly pure silica gel base
T
wo types of test reports are included
Pore size80
Carbon content22
Usable pH range1.0 10.0
Excellent durability
The
pH 1
pH 10
100
100
80
80
60
60
20
40
( )
40
C18 RS
ODS
Pro C18 RS
Monomeric ODS
20
0
20
40
60
80
100(hours)
Hours exposure to acetonitrile/water/TFA (10/90/1),
change in packing material hydrophobicity at 70 .
0
200
400
600
800(hours)
Sequential analysis using 20 mM H3BO3-NaOH (pH 9.8)/methanol (50/50)
pH 3.2
3,4,5
504.6 mmI.D. 3 m
pH 6.8
pH 8.8
pH 8.8
Downsizing
1 2
12
F040716A
10
1. Flurbiprofen
2. Methyl benzoate
3. Lidocaine
4. Diltiazem
5. Verapamil
15
12 3 4
20 min
F040714E
10
15
20 min
Column
YMC-Pack Pro C18 RS
Eluent
20 mM acetate buffer/methanol (35/65)
Flow rate 1.0 mL/min
Temperature 30
Detection UV at 220 nm
F040714C
10
15
20 min
F040714D
10 min
40
01.indd 40
11.1.28 10:41:12 AM
C18 RS
Tf(4) 2.87
3
1
0
10
J011114B
20 min
10
J020523D
20 min
1. Desipramine
2. Diphenyl (I.S.)
3. Imipramine
4. Amitriptyline
Column
1504.6 mmI.D.
Eluent
20 mM KH2PO4-K2HPO4 (pH 6.9)
/methanol (25/75)
Flow rate 1.0 mL/min
Temperature 37
Detection UV at 254 nm
C18 RS
5 m
Brand F1
(standard-type ODS 5 m)
ODS
Tf(4) 1.16
Rs=1.24
Rs=0.8
F040713D
10
20
30
min
0 F040714
10
20
30 min
Column
1504.6 mmI.D.
Eluent
acetonitrile/THF/water (35/30/35)
Flow rate 1.0 mL/min
Temperature 30
Detection UV at 210 nm
C18 RS
3 m
1
Rs=1.54
F040713A
10
20
30 min
41
01.indd 41
11.1.28 10:41:15 AM
Analytical columns
530.95
M+2
100
%
ODS
Bradykinin(MW: 1059.56)
531.47
M+1
1060.27
400
600
800
100
18
5.00
1000
3 5
1200(m/z)
1. [Arg8]-Vasopressin
2. Bradykinin
3. Angiotensin
4. Oxytocin
5. Angiotensin
6. Leu-Enkephalin
10.00
15.00
Column
YMC-Pack Pro C18 RS (5 m, 80 )
1502.0 mmI.D.
Eluent
A) water/TFA (100/0.01)
B) acetonitrile/TFA (100/0.01)
10-35% B (0-15 min)
Flow rate 0.2 mL/min
Temperature 37
Detection ESI positive-mode
20.00
Time
Application
Chloroacetophenone isomers
1.
Chlorophenol isomers
COCH3
Cl
1.
o-Chloroacetophenone
2.
3.
2.
J011026B
10
20 min
3.
Cl
m-Chloroacetophenone
OH
Cl
p-Chlorophenol
COCH3
Column
YMC-Pack Pro C18 RS (5 m, 80 )
1504.6 mmI.D.
Eluent
acetonitrile/water (40/60)
Flow rate 1.0 mL/min
Temperature 30
Detection UV at 254 nm
Cl
o-Chlorophenol
COCH3
Cl
p-Chloroacetophenone
OH
OH
Cl
m-Chlorophenol
Column
Eluent
J011113D
10
20 min
42
01.indd 42
11.1.28 10:41:19 AM
Ordering information
Analytical columns
Particle size
m
S-3
inner diameterlengthmm
80
80
1.0 35
1.0 50
1.0100
1.5 35
1.5 50
1.5100
2.0 35
2.0 50
2.0 75
2.0100
2.0150
3.0 50
3.0100
3.0150
4.6 35
4.6 50
4.6 75
4.6100
4.6150
6.0 50
6.0 75
6.0100
1.0150
1.0250
1.5150
1.5250
2.0 35
2.0 50
2.0 75
2.0100
2.0150
2.0250
3.0 50
3.0 75
3.0100
3.0150
3.0250
4.6 50
4.6 75
4.6100
4.6150
4.6250
6.0150
6.0250
Product number
RS08S03-H501WT
RS08S03-0501WT
RS08S03-1001WT
RS08S03-H5P5WT
RS08S03-05P5WT
RS08S03-10P5WT
RS08S03-H502WT
RS08S03-0502WT
RS08S03-L502WT
RS08S03-1002WT
RS08S03-1502WT
RS08S03-0503WT
RS08S03-1003WT
RS08S03-1503WT
RS08S03-H546WT
RS08S03-0546WT
RS08S03-L546WT
RS08S03-1046WT
RS08S03-1546WT
RS08S03-0506WT
RS08S03-L506WT
RS08S03-1006WT
RS08S05-1501WT
RS08S05-2501WT
RS08S05-15P5WT
RS08S05-25P5WT
RS08S05-H502WT
RS08S05-0502WT
RS08S05-L502WT
RS08S05-1002WT
RS08S05-1502WT
RS08S05-2502WT
RS08S05-0503WT
RS08S05-L503WT
RS08S05-1003WT
RS08S05-1503WT
RS08S05-2503WT
RS08S05-0546WT
RS08S05-L546WT
RS08S05-1046WT
RS08S05-1546WT
RS08S05-2546WT
RS08S05-1506WT
RS08S05-2506WT
Particle size
m
S-5
inner diameterlengthmm
80
10150
10250
20100
20150
20250
Product number
RS08S05-1510WT
RS08S05-2510WT
RS08S05-1020WT
RS08S05-1520WT
RS08S05-2520WT
Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)
RS08S03-0101CC
1.010
S-3
80
S-5
80
1.510
2.010
4.023
1.010
1.510
2.010
4.023
RS08S03-01P5CC
RS08S03-0102CC
RS08S03-G304CC
RS08S05-0101CC
RS08S05-01P5CC
RS08S05-0102CC
RS08S05-G304CC
A guard cartridge holder will need to be purchased separately before using this
product for the first time.
Cartridge holder
Semi-micro guard cartridge holder
(inner diameter: 1.0, 1.5, or 2.0 mm)
Cartridge holder
(inner diameter: 4.0 mm)
80
4.023
ODS
S-5
Semi-preparative columns
XPCHSMW
XPCHW
RS08S03-G304CCPK
RS08S05-G304CCPK
Guard columns
S-5
80
1030
2050
RS08S05-0310WTG
RS08S05-0520WTG
43
01.indd 43
11.1.28 10:41:20 AM
Analytical columns
YMC-Pack ODS-A
Conventional ODS column
Currently in use worldwide
Standard ODS
YMC-Pack ODS-A has a highly endcapped surface
structure and appropriate hydrophobicity for separation
of a wide range of compounds. It is produced under strict
quality control with respect to 50 or more parameters in
10
ODS
8
6
4
2
0
4 5 6 7
Lot Number
9 10
Application
S930422D
Application
Analgesics
O
CH3COHN
OH
OC2H5
Ethenzamide
CH3
N
N
N
CH3
1.-Carotene
2.-Carotene
Caffeine
CONH2
H 3C
O
Acetaminophen
Carotenes
CH2= CHCH2
H 3C
H 3C
CHCHCONHCONH 2
Apronalide
10
20
30 min
G910523Q
Column
YMC-Pack ODS-A
(5 m, 120 )
1504.6 mmI.D.
Eluent
methanol/water (40/60)
Flow rate 0.7 mL/min
Temperature 30
Detection UV at 220 nm
10
20
min
Column
YMC-Pack ODS-A
(5 m, 120 )
1504.6 mmI.D.
Eluent
acetonitrile/methanol/THF
(50/45/5)
Flow rate 1.0 mL/min
Temperature 30
Detection UV at 450 nm
Ordering information
Analytical columns
Particle size
m
S-3
inner diameterlengthmm
120
2.0 35
2.0 50
2.0 75
2.0100
2.0150
3.0 50
3.0100
3.0150
Product number
AA12S03-H502WT
AA12S03-0502WT
AA12S03-L502WT
AA12S03-1002WT
AA12S03-1502WT
AA12S03-0503WT
AA12S03-1003WT
AA12S03-1503WT
Particle size
m
S-3
inner diameterlengthmm
120
4.6 75
4.6100
4.6150
6.0100
6.0150
Product number
AA12S03-L546WT
AA12S03-1046WT
AA12S03-1546WT
AA12S03-1006WT
AA12S03-1506WT
44
01.indd 44
11.1.28 10:41:23 AM
Ordering information
Semi-preparative columns
Analytical columns
Particle size
m
S-5
inner diameterlengthmm
120
300
S-10
120
300
AA12S05-1501WT
AA12S05-2501WT
AA12S05-15P5WT
AA12S05-25P5WT
AA12S05-L502WT
AA12S05-1502WT
AA12S05-2502WT
AA12S05-1503WT
AA12S05-2503WT
AA12S05-L546WT
AA12S05-1046WT
AA12S05-1546WT
AA12S05-2546WT
AA12S05-3046WT
AA12S05-1006WT
AA12S05-1506WT
AA12S05-2506WT
AA12S05-3006WT
AA20S05-1546WT
AA20S05-2546WT
AA30S05-1501WT
AA30S05-2501WT
AA30S05-15P5WT
AA30S05-25P5WT
AA30S05-L502WT
AA30S05-1502WT
AA30S05-2502WT
AA30S05-L546WT
AA30S05-1046WT
AA30S05-1546WT
AA30S05-2546WT
AA30S05-3046WT
AA30S05-1006WT
AA30S05-1506WT
AA30S05-2506WT
AA30S05-3006WT
AA12S11-1046WT
AA12S11-1546WT
AA12S11-2546WT
AA12S11-3046WT
AA12S11-1006WT
AA12S11-1506WT
AA12S11-2506WT
AA12S11-3006WT
AA30S11-1046WT
AA30S11-1546WT
AA30S11-2546WT
AA30S11-3046WT
AA30S11-1006WT
AA30S11-1506WT
AA30S11-2506WT
AA30S11-3006WT
Particle size
m
S-10
S-5
inner diameterlengthmm
120
200
300
10150
10250
10300
20100
20150
20250
20150
20250
10150
10250
10300
20150
20250
120
200
300
AA12S11-1510WT
AA12S11-2510WT
AA12S11-3010WT
AA12S11-1520WT
AA12S11-2520WT
AA20S11-1520WT
AA20S11-2520WT
AA30S11-1510WT
AA30S11-2510WT
AA30S11-3010WT
AA30S11-1520WT
AA30S11-2520WT
Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)
AA12S03-0102CC
2.010
S-3
S-5
120
200
300
4.023
1.010
1.510
2.010
4.023
4.023
1.010
1.510
2.010
4.023
AA12S03-G304CC
AA12S05-0101CC
AA12S05-01P5CC
AA12S05-0102CC
AA12S05-G304CC
AA20S05-G304CC
AA30S05-0101CC
AA30S05-01P5CC
AA30S05-0102CC
AA30S05-G304CC
A guard cartridge holder will need to be purchased separately before using this
product for the first time.
Cartridge holder
Semi-micro guard cartridge holder
(inner diameter: 1.0, 1.5, or 2.0 mm)
Cartridge holder
(inner diameter: 4.0 mm)
XPCHSMW
XPCHW
120
4.023
AA12S03-G304CCPK
S-5
120
4.023
AA12S05-G304CCPK
Guard columns
S-3
120
S-5
120
200
Product number
AA12S05-1510WT
AA12S05-2510WT
AA12S05-3010WT
AA12S05-1020WT
AA12S05-1520WT
AA12S05-2520WT
AA20S05-1520WT
AA20S05-2520WT
AA30S05-1510WT
AA30S05-2510WT
AA30S05-3010WT
AA30S05-1520WT
AA30S05-2520WT
10150
10250
10300
20150
20250
20150
20250
10150
10250
10300
20150
20250
Product number
See P145 for preparative columns other than those listed above.
Semi-preparative columns
Particle size
m
inner diameterlengthmm
ODS
200
1.0150
1.0250
1.5150
1.5250
2.0 75
2.0150
2.0250
3.0150
3.0250
4.6 75
4.6100
4.6150
4.6250
4.6300
6.0100
6.0150
6.0250
6.0300
4.6150
4.6250
1.0150
1.0250
1.5150
1.5250
2.0 75
2.0150
2.0250
4.6 75
4.6100
4.6150
4.6250
4.6300
6.0100
6.0150
6.0250
6.0300
4.6100
4.6150
4.6250
4.6300
6.0100
6.0150
6.0250
6.0300
4.6100
4.6150
4.6250
4.6300
6.0100
6.0150
6.0250
6.0300
Product number
300
S-10
120
200
300
4.010
5.010
4.010
5.010
1030
2050
4.010
2050
4.010
5.010
1030
2050
4.010
5.010
1030
2050
4.010
5.010
2050
4.010
5.010
1030
2050
AA12S03-0104WFG
AA12S03-0105WFG
AA12S05-0104WFG
AA12S05-0105WFG
AA12S05-0310WTG
AA12S05-0520WTG
AA20S05-0104WFG
AA20S05-0520WTG
AA30S05-0104WFG
AA30S05-0105WFG
AA30S05-0310WTG
AA30S05-0520WTG
AA12S11-0104WFG
AA12S11-0105WFG
AA12S11-0310WTG
AA12S11-0520WTG
AA20S11-0104WFG
AA20S11-0105WFG
AA20S11-0520WTG
AA30S11-0104WFG
AA30S11-0105WFG
AA30S11-0310WTG
AA30S11-0520WTG
45
01.indd 45
11.1.28 10:41:24 AM
Analytical columns
YMC-Pack ODS-AM
Similar to ODS-A in selectivity
Excellent reproducibility
Useful for quality control purposes
Pore size120
Carbon content17
Usable pH range2.0 7.5
(Methylparben/2,6-Dimethylpyridine)
ODS
10
Lot Number
Acidic compounds
Coordination compounds
1. Uracil
2. Benzoic acid
3. p-Toluic acid
4. p-Ethylbenzoic acid
5. Methy benzoate
1. Uracil
2. Acetylacetone
3. 8-Quinolinol
ODS-AM
3
2
2
4
46
01.indd 46
11.1.28 10:41:26 AM
Application
G910523J
Alkaloids
1
4
3
7
5
9
8
6
20
40
60
80
min
Column
YMC-Pack ODS-AM (5 m, 120 )
1504.6 mmI.D.
Eluent
acetonitrile/20 mM KH2PO4 (20/80)
Flow rate 1.0 mL/min
Temperature 30
Detection UV at 210 nm
Ordering information
Analytical columns
Particle size
m
S-3
S-5
Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)
inner diameterlengthmm
120
120
2.0 35
2.0 50
2.0 75
2.0100
2.0150
3.0 50
3.0100
3.0150
4.6 75
4.6100
4.6150
6.0100
6.0150
6.0250
1.0150
1.0250
1.5150
1.5250
2.0 75
2.0100
2.0150
2.0250
3.0150
3.0250
4.6 75
4.6100
4.6150
4.6250
4.6300
6.0100
6.0150
6.0250
6.0300
Semi-preparative columns
10150
S-5
120
10250
10300
20100
20150
20250
Product number
AM12S03-H502WT
AM12S03-0502WT
AM12S03-L502WT
AM12S03-1002WT
AM12S03-1502WT
AM12S03-0503WT
AM12S03-1003WT
AM12S03-1503WT
AM12S03-L546WT
AM12S03-1046WT
AM12S03-1546WT
AM12S03-1006WT
AM12S03-1506WT
AM12S03-2506WT
AM12S05-1501WT
AM12S05-2501WT
AM12S05-15P5WT
AM12S05-25P5WT
AM12S05-L502WT
AM12S05-1002WT
AM12S05-1502WT
AM12S05-2502WT
AM12S05-1503WT
AM12S05-2503WT
AM12S05-L546WT
AM12S05-1046WT
AM12S05-1546WT
AM12S05-2546WT
AM12S05-3046WT
AM12S05-1006WT
AM12S05-1506WT
AM12S05-2506WT
AM12S05-3006WT
AM12S05-1510WT
AM12S05-2510WT
AM12S05-3010WT
AM12S05-1020WT
AM12S05-1520WT
AM12S05-2520WT
See P147 for preparative columns other than those listed above.
01.indd 47
Particle size
m
inner diameterlengthmm
S-3
120
S-5
120
2.010
4.023
1.010
1.510
2.010
4.023
ODS
1. Hordenine sulfate
2. Pilocarpine chloride
3. Scopolamine bromide
4. Atropine sulfate
5. Cinchonine
6. Sparteine sulfate
7. Quinine
8. Papaverine chloride
9. Narcotine chloride
Product number
AM12S03-0102CC
AM12S03-G304CC
AM12S05-0101CC
AM12S05-01P5CC
AM12S05-0102CC
AM12S05-G304CC
A guard cartridge holder will need to be purchased separately before using this
product for the first time.
Cartridge holder
Semi-micro guard cartridge holder
(inner diameter: 1.0, 1.5, or 2.0 mm)
Cartridge holder
(inner diameter: 4.0 mm)
XPCHSMW
XPCHW
120
4.023
AM12S03-G304CCPK
S-5
120
4.023
AM12S05-G304CCPK
Guard columns
S-3
120
S-5
120
4.010
5.010
4.010
5.010
1030
2050
AM12S03-0104WFG
AM12S03-0105WFG
AM12S05-0104WFG
AM12S05-0105WFG
AM12S05-0310WTG
AM12S05-0520WTG
47
11.1.28 10:41:27 AM
Analytical columns
YMC-Pack ODS-AQ
Useful for separation of hydrophilic compounds
Can be used with 100% water in mobile phase
Different selectivity from conventional ODS
Hydrophilic ODS
YMC-Pack ODS-AQ has moderate hydrophobicity and
hydrogen-bonding capacity. It shows different retention
behavior from that of YMC-Pack ODS-A, for samples
with relatively high hydrophilicity.
ODS
G11
G12
G13
1. MaltoundecaoseG11
2. MaltododecaoseG12
3. MaltotridecaoseG13
4. MaltotetradecaoseG14
5. MaltopentadecaoseG15
G14
G15
10
20
30
Column
YMC-Pack ODS-AQ (5 m, 120 )
1504.6 mmI.D.
Eluent
methanol/water (5/995)
Flow rate 1.0 mL/min
Temperature 37
Detection RI, 810-6RIU/FS
min
M910604M
Hydrogen-bonding capacity
ODS-A
ODS-AQ
ODS-AL
2
3
3
4
5
4
1. Trigonelline
2. Theobromine
3. Theophylline
4. Caffeine
5. Phenol
(Phenol/Caffeine)
1.8
ODS-A
1.6
1.4
ODS-AQ
1.2
ODS-AL
1.0
3.0
(60 )
3.1
3.2
3
10 /T,K
3.3
-1
3.4
(20 )
48
01.indd 48
11.1.28 10:41:30 AM
Application
G910607E
1. Arbutin
2. Salicin
3. Esculine
4. G-Strophantine(Ouabain)
5. Baicalin
6. Barbaloin
7. Glycyrrhizin
8. Digitoxine
5
34
2
7
8
10
20
30
40
min
Ordering information
Analytical columns
Particle size
m
S-3
S-5
Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)
inner diameterlengthmm
120
120
200
2.0 35
2.0 50
2.0 75
2.0100
2.0150
3.0 50
3.0100
3.0150
4.6100
4.6150
6.0100
6.0150
1.0150
1.0250
1.5150
1.5250
2.0 75
2.0150
2.0250
3.0150
3.0250
4.6 75
4.6100
4.6150
4.6250
4.6300
6.0100
6.0150
6.0250
6.0300
4.6150
4.6250
Semi-preparative columns
10150
S-5
120
200
S-10
120
200
10250
10300
20100
20150
20250
20150
20250
10150
10250
10300
20150
20250
20150
20250
Product number
AQ12S03-H502WT
AQ12S03-0502WT
AQ12S03-L502WT
AQ12S03-1002WT
AQ12S03-1502WT
AQ12S03-0503WT
AQ12S03-1003WT
AQ12S03-1503WT
AQ12S03-1046WT
AQ12S03-1546WT
AQ12S03-1006WT
AQ12S03-1506WT
AQ12S05-1501WT
AQ12S05-2501WT
AQ12S05-15P5WT
AQ12S05-25P5WT
AQ12S05-L502WT
AQ12S05-1502WT
AQ12S05-2502WT
AQ12S05-1503WT
AQ12S05-2503WT
AQ12S05-L546WT
AQ12S05-1046WT
AQ12S05-1546WT
AQ12S05-2546WT
AQ12S05-3046WT
AQ12S05-1006WT
AQ12S05-1506WT
AQ12S05-2506WT
AQ12S05-3006WT
AQ20S05-1546WT
AQ20S05-2546WT
Particle size
m
S-3
120
S-5
120
200
2.010
4.023
1.010
1.510
2.010
4.023
4.023
Product number
AQ12S03-0102CC
AQ12S03-G304CC
AQ12S05-0101CC
AQ12S05-01P5CC
AQ12S05-0102CC
AQ12S05-G304CC
AQ20S05-G304CC
A guard cartridge holder will need to be purchased separately before using this
product for the first time.
Cartridge holder
Semi-micro guard cartridge holder
(inner diameter: 1.0, 1.5, or 2.0 mm)
Cartridge holder
(inner diameter: 4.0 mm)
XPCHSMW
XPCHW
120
4.023
AQ12S03-G304CCPK
S-5
120
4.023
AQ12S05-G304CCPK
Guard columns
S-3
120
S-5
AQ12S05-1510WT
AQ12S05-2510WT
AQ12S05-3010WT
AQ12S05-1020WT
AQ12S05-1520WT
AQ12S05-2520WT
AQ20S05-1520WT
AQ20S05-2520WT
AQ12S11-1510WT
AQ12S11-2510WT
AQ12S11-3010WT
AQ12S11-1520WT
AQ12S11-2520WT
AQ20S11-1520WT
AQ20S11-2520WT
inner diameterlengthmm
ODS
Column
YMC-Pack ODS-AQ (5 m, 120 )
2504.6 mmI.D.
Eluent
A) methanol/20 mM NH4H2PO4 (5/95)
B) methanol/20 mM NH4H2PO4 (80/20)
0-100 B (0-20 min,linear),100 B (20-40 min)
Flow rate 0.6 mL/min
Temperature 30
Detection UV at 250 nm
120
200
S-10
120
200
4.010
5.010
4.010
5.010
1030
2050
4.010
2050
1030
2050
2050
AQ12S03-0104WFG
AQ12S03-0105WFG
AQ12S05-0104WFG
AQ12S05-0105WFG
AQ12S05-0310WTG
AQ12S05-0520WTG
AQ20S05-0104WFG
AQ20S05-0520WTG
AQ12S11-0310WTG
AQ12S11-0520WTG
AQ20S11-0520WTG
See P146 for preparative columns other than those listed above.
49
01.indd 49
11.1.28 10:41:32 AM
Analytical columns
YMC-Pack ODS-AL
ODS with residual silanol groups
Utilizes secondary interaction caused by silanol groups
Pore size120
Carbon content17
Usable pH range2.0 7.5
Non-endcapped ODS
YMC-Pack ODS-AL uses not only hydrophobic
interaction but also secondary interaction caused by
silanol groups that affect separation. This results in a
different selectivity from conventional ODS columns.
ODS
ODS-AM
2 3
3
(4/3)=2.80
(4/3)=1.46
1. Uracil
2. Pyridine
3. 2,6-Dimethylpyridine
4. Methylparaben
4
4
ODS-AL
2,3
3
1
Disinfectants
1.
COOH
2.
COOH
OH
Benzoic acid
Salicylic acid
3.
4.
H3C
H
N
OH
CH 3
Theophylline (I.S.)
10
15
20
min
10
15
20
min
Phenol
Column
YMC-Pack ODS-AL and ODS-AQ (5 m, 120 )
1504.6 mmI.D.
Eluent
100 mM KH2PO4-Na2HPO4 (pH 7.0)/methanol (75/25)
Flow rate 0.8 mL/min
Temperature 30
Detection UV at 270 nm
(Conditions described in Japanese Pharmacopoeia 15th ed.)
50
01.indd 50
11.1.28 10:41:35 AM
Application
G900417B
ODS
Column
10
20
30
40
min
Ordering information
Analytical columns
Particle size
m
S-5
Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)
inner diameterlengthmm
120
1.0150
1.0250
1.5150
1.5250
2.0150
2.0250
4.6 75
4.6100
4.6150
4.6250
4.6300
6.0100
6.0150
6.0250
6.0300
Semi-preparative columns
10150
S-5
120
10250
10300
20100
20150
20250
Product number
AL12S05-1501WT
AL12S05-2501WT
AL12S05-15P5WT
AL12S05-25P5WT
AL12S05-1502WT
AL12S05-2502WT
AL12S05-L546WT
AL12S05-1046WT
AL12S05-1546WT
AL12S05-2546WT
AL12S05-3046WT
AL12S05-1006WT
AL12S05-1506WT
AL12S05-2506WT
AL12S05-3006WT
AL12S05-1510WT
AL12S05-2510WT
AL12S05-3010WT
AL12S05-1020WT
AL12S05-1520WT
AL12S05-2520WT
Particle size
m
S-5
inner diameterlengthmm
120
1.010
1.510
2.010
4.023
Product number
AL12S05-0101CC
AL12S05-01P5CC
AL12S05-0102CC
AL12S05-G304CC
A guard cartridge holder will need to be purchased separately before using this
product for the first time.
Cartridge holder
Semi-micro guard cartridge holder
(inner diameter: 1.0, 1.5, or 2.0 mm)
Cartridge holder
(inner diameter: 4.0 mm)
Guard columns
S-5
120
4.010
5.010
1030
2050
XPCHSMW
XPCHW
AL12S05-0104WFG
AL12S05-0105WFG
AL12S05-0310WTG
AL12S05-0520WTG
See P147 for preparative columns other than those listed above.
51
01.indd 51
11.1.28 10:41:36 AM
Analytical columns
Pore size80
Carbon contentODS-H80 22 , ODS-M80 14% , ODS-L80 9
Usable pH rangeODS-H80 1.0 9.0, ODS-M80L80 2.0 7.5
ODS-H80
ODS-M80
ODS-L80
ODS-L80
ODS-M80
Column
J
sphere
1504.6 mmI.D.
Eluent
acetonitrile/water (75/25)
Flow rate 1.0 mL/min
Temperature 37
Detection UV at 254 nm
ODS-H80
0
10
20
30
40
50 min
10
20
k(octylbenzene)
0.20
0.25
(caffeine/phenol)
29.6
Column
1504.6 mmI.D.
Eluent
acetonitrile/water (75/25)
Flow rate
1.0 mL/min
Temperature 37
Detection UV at 254 nm
30
0.30
0.30
Column
1504.6 mmI.D.
Eluent
acetonitrile/20 mM KH2PO4 (15/85)
Flow rate
1.0 mL/min
Temperature 37
Detection UV at 254 nm
0.30
52
01.indd 52
11.1.28 10:41:39 AM
Ordering information
Analytical columns
Semi-preparative columns
ODS-H80
ODS-L80
S-4
S-4
80
80
80
1.0150
1.0250
1.5150
1.5250
2.0 75
2.0100
2.0150
2.0250
3.0150
3.0250
4.6 75
4.6150
4.6250
6.0150
6.0250
1.0150
1.0250
1.5150
1.5250
2.0 75
2.0100
2.0150
2.0250
3.0150
3.0250
4.6 75
4.6150
4.6250
6.0150
6.0250
1.0150
1.0250
1.5150
1.5250
2.0 35
2.0 75
2.0100
2.0150
2.0250
3.0150
3.0250
4.6 75
4.6150
4.6250
6.0150
6.0250
JH08S04-1501WT
JH08S04-2501WT
JH08S04-15P5WT
JH08S04-25P5WT
JH08S04-L502WT
JH08S04-1002WT
JH08S04-1502WT
JH08S04-2502WT
JH08S04-1503WT
JH08S04-2503WT
JH08S04-L546WT
JH08S04-1546WT
JH08S04-2546WT
JH08S04-1506WT
JH08S04-2506WT
JM08S04-1501WT
JM08S04-2501WT
JM08S04-15P5WT
JM08S04-25P5WT
JM08S04-L502WT
JM08S04-1002WT
JM08S04-1502WT
JM08S04-2502WT
JM08S04-1503WT
JM08S04-2503WT
JM08S04-L546WT
JM08S04-1546WT
JM08S04-2546WT
JM08S04-1506WT
JM08S04-2506WT
JL08S04-1501WT
JL08S04-2501WT
JL08S04-15P5WT
JL08S04-25P5WT
JL08S04-H502WT
JL08S04-L502WT
JL08S04-1002WT
JL08S04-1502WT
JL08S04-2502WT
JL08S04-1503WT
JL08S04-2503WT
JL08S04-L546WT
JL08S04-1546WT
JL08S04-2546WT
JL08S04-1506WT
JL08S04-2506WT
ODS-H80
S-4
80
ODS-M80
S-4
80
ODS-L80
S-4
80
10150
10250
20150
20250
10150
10250
20150
20250
10150
10250
20150
20250
Product number
JH08S04-1510WT
JH08S04-2510WT
JH08S04-1520WT
JH08S04-2520WT
JM08S04-1510WT
JM08S04-2510WT
JM08S04-1520WT
JM08S04-2520WT
JL08S04-1510WT
JL08S04-2510WT
JL08S04-1520WT
JL08S04-2520WT
Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)
JH08S04-0101CC
1.010
ODS-H80 S-4
80
ODS-M80
S-4
80
ODS-L80
S-4
80
1.510
2.010
4.023
1.010
1.510
2.010
4.023
1.010
1.510
2.010
4.023
JH08S04-01P5CC
JH08S04-0102CC
JH08S04-G304CC
JM08S04-0101CC
JM08S04-01P5CC
JM08S04-0102CC
JM08S04-G304CC
JL08S04-0101CC
JL08S04-01P5CC
JL08S04-0102CC
JL08S04-G304CC
ODS
ODS-M80
S-4
Product number
A guard cartridge holder will need to be purchased separately before using this
product for the first time.
Cartridge holder
Semi-micro guard cartridge holder
(inner diameter: 1.0, 1.5, or 2.0 mm)
Cartridge holder
(inner diameter: 4.0 mm)
XPCHSMW
XPCHW
JH08S04-G304CCPK
ODS-M80
S-4
80
4.023
JM08S04-G304CCPK
ODS-L80
S-4
80
4.023
JL08S04-G304CCPK
Guard columns
ODS-H80 S-4
80
ODS-M80
S-4
80
ODS-L80
S-4
80
1030
2050
1030
2050
1030
2050
JH08S04-0310WTG
JH08S04-0520WTG
JM08S04-0310WTG
JM08S04-0520WTG
JL08S04-0310WTG
JL08S04-0520WTG
53
01.indd 53
11.1.28 10:41:40 AM
Analytical columns
YMC-Pack PolymerC18
Utilizes polymer base which is not affected by silanol
Different separation characteristics from silica-based ODS
Excellent pH stability
P o l y m e r t y p e C18
PolymerC18 is a C18 column made from methacrylate
polymer. It has excellent pH stability and it is useful for
separation of basic compounds because there are no
silanol or metal impurities to cause secondary interaction.
ODS
1. Barbital
2. Phenobarbital
3. Pentobarbital
4. Hexobarbital
5. Secobarbital
10
20 min
Column
YMC-Pack PolymerC18
1504.6 mmI.D.
Eluent
50 mM Na2HPO4-Na3PO4(pH 11.0)/methanol (75/25)
Flow rate 0.5 mL/min
Temperature 30
Detection UV at 254 nm
Acid resistance
2
3
10
20 min
10
2
3
20 min
Flow conditions
Column
YMC-Pack PolymerC18 1504.6 mmI.D.
Eluent
0.1N NaOH (pH 13)/acetonitrile (80/20)
Flow rate
0.5 mL/min
Temperature ambient
Time
6 hours
Analytical conditions
Eluent
acetonitrile/water (60/40)
Flow rate
0.5 mL/min
Temperature 30
Detection UV at 270 nm
Sample
1.Uracil
2.Methyl benzoate
3.Butyl benzoate
10
20 min
10
20 min
Flow conditions
Column
YMC-Pack PolymerC18 1504.6 mmI.D.
Eluent
100 mM H3PO4 (pH 1.7)
Flow rate
0.5 mL/min
Temperature ambient
Time
100 hours
Analytical conditions
Eluent
acetonitrile/water (60/40)
Flow rate
0.5 mL/min
Temperature 30
Detection UV at 270 nm
Sample
1.Uracil
2.Methyl benzoate
3.Butyl benzoate
54
01.indd 54
11.1.28 10:41:43 AM
YMC-Pack ODS-A
YMC-Pack PolymerC18
1.
3
HO
2.
OH
OH
OH
OH
Phloroglucinol
3.
OH
Resorcinol
Phenol
1
3
10
20
30 min
Column
YMC-Pack PolymerC18 and YMC-Pack ODS-A
1506.0 mmI.D.
Eluent
methanol/water (40/60)
Flow rate 1.0 mL/min
Temperature 30
Detection UV at 280 nm
5 10 min
Triterpene saponins
YMC-Pack ODS-AM
1.
2.
19
ODS
18
1,2
COO--D-Glc
HO
COO--D-Glc
HO
HO
HO
CH2 OH
CH2 OH
0.00
7.50
15.00
22.50 min
0.00
7.50
15.00
22.50
min
Column
YMC-Pack PolymerC18 and YMC-Pack ODS-AM
1504.6 mmI.D.
Eluent
methanol/water (45/55) for PolymerC18
methanol/water (50/50) for ODS-AM
Flow rate 0.8 mL/min
Temperature 50
Detection UV at 210 nm
Ordering information
Analytical columns
Guard cartridge columns (with inner diameter 2.0 mm: 2/pack, 4.0 mm: 3/pack)
Particle size
m
Column size
inner diameterlengthmm
Product number
S-6
2.0 75
2.0150
4.6150
4.6250
6.0150
6.0250
4.6250
4.6300
6.0250
6.0300
PC99S06-L502WT
PC99S06-1502WT
PC99S06-1546WT
PC99S06-2546WT
PC99S06-1506WT
PC99S06-2506WT
PC99S10-2546WT
PC99S10-3046WT
PC99S10-2506WT
PC99S10-3006WT
S-10
Semi-preparative columns
S-6
S-10
10250
PC99S06-2510WT
10250
20300
PC99S10-2510WT
PC99S10-3020WT
Particle size
m
Column size
inner diameterlengthmm
Product number
S-6
2.010
4.023
PC99S06-0102CC
PC99S06-G304CC
A guard cartridge holder will need to be purchased separately before using this
product for the first time.
Cartridge holder
Semi-micro guard cartridge holder
(inner diameter: 1.0, 1.5, or 2.0 mm)
Cartridge holder
(inner diameter: 4.0 mm)
Guard columns
S-6
S-10
4.010
5.010
1030
4.010
5.010
1030
2050
XPCHSMW
XPCHW
PC99S06-0104WFG
PC99S06-0105WFG
PC99S06-0310WTG
PC99S10-0104WFG
PC99S10-0105WFG
PC99S10-0310WTG
PC99S10-0520WTG
55
01.indd 55
11.1.28 10:41:47 AM
01.indd 56
11.1.28 10:41:47 AM