WORLD

AQUACULTURE 2015
Aquaculture For Healthy People, Planet and Profit

May 26-30, 2015

Jeju Exhibition & Convention Center
Jeju, Korea

THE ANNUAL INTERNATIONAL CONFERENCE & EXPOSITION OF

WORLD AQUACULTURE SOCIETY
ASIAN-PACIFIC CHAPTER, WAS
KOREAN CHAPTER, WAS
HOSTED BY
Korean Society of Fisheries and Aquatic Science
Jeju Special Self-governing Province
Jeju Special Self-governing Provincial Council
Jeju Fish-Culture Fisheries Cooperatives

www.was.org

ASSOCIATE SPONSORS
Aquaculture Engineering Society
International Association of Aquaculture Economics &
Management

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Next generation sequencing uncovers

unexpected pathogens associated with early
mortality syndrome (EMS) in shrimp
Kallaya Sritunyalucksana1,
Anuphap Prachumwat1,
Sissades Tongsima2,
Siripong Thitamadee3,
Timothy W. Flegel4

Shrimp-virus Interaction Laboratory (ASVI), National Center for Genetic Engineering and Biotechnology,
National Science and Technology Development Agency, Bangkok 10400, Thailand
2
Biostatistics and Informatics Laboratory, Genome Institute National Center for GeneticEngineering and
Biotechnology, Pathum Thani 12120, Thailand
3
Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400 Thailand
4
National Center for Genetic Engineering and Biotechnology, National Science and Technology
Development Agency, Pathum Thani 12120, Thailand
E-mail: kallaya@biotec.or.th
1

Presented in "Next Generation Sequencing for Genetic and Genomic Studies (NGS2014)" organized by Mahidol University,
29-30 July, 2014 Winser Hotel, Bangkok, Thailand

pathogens, we also found the concurrent problems in shrimp associated

with EMS including the occurrence of aggregated transformed microvilli
(ATM). Preliminary study using the next generation sequencing and
bioinformatic analysis suggested that there might be the pathogen
involved. Further investigations
on these pathogens are
urgently required in order to
design the appropriate strategy
to control EMS in shrimp
industry.

ACKNOWLEDGEMENTS:

We thank National Research

Council of Thailand
(NRCT), National Center for
Genetic Engineering and
Biotechnology (BIOTEC)
and Agricultural Research
Development Agency (ARDA)
for grant support for the study
on shrimp EMS.

NEXT GENERATION GENOMIC STUDIES

The most threatening new problem in Asian shrimp aquaculture since

2009 has been serious mortality within approximately the first 35
days after stocking shrimp ponds with post larvae. The problem is
commonly called early mortality syndrome (EMS) and started at the
early of 2012 in Thailand and has
caused Thai shrimp production to
reduce 54% in 2013. The cause of
EMS was originally unknown. We
carried out a metagenomic analysis
to determine whether any unique
bacteria (culturable or unculturable)
might be present in EMS ponds
when compared to normal ponds.
DNA was extracted from pooled HP
tissue samples from normal and
EMS shrimp ponds and subjected to
PCR amplification of small subunit
ribosomal RNA (ssu rRNA) gene
fragments using universal bacterial
primers. After primer and multiplex
adapter sequence trimming, the
remaining 59.7 million bases from
163,102 reads with lengths of at least
100 bp were used to construct unique
sequences (operational taxonomic
units, OTUs). Since no OTU were
found to be exclusive to EMS ponds,
we compared the proportion of reads
for OTU between EMS ponds and
normal ponds. This comparison revealed 32 OTUs of six genera from
four algorithms that had a significantly higher proportion of reads in
EMS ponds than in normal ponds. This comparison revealed OTU with
high identity to expected Vibrio species (e.g., V. parahaemolyticus, V.
harveyi and V. campbellii) and with high identity to other bacteria known
from aquatic environments, but never previously reported as shrimp
pathogens. The latter included the genera Delftia and Ralstonia from the
order Berkholderiales and Leifsonia and Rhodococcus from the Order
Actinomycetales. Preliminary studies with bioassay confirmed that
these bacteria could be direct shrimp pathogens. Besides the bacterial

11

Case 11-041. Normal hepatopancreas (HP)

Uneaten Feed
Bacteria
No2 Nitrite

Mineralization

Bacteria

Algae bloom

No2 Nitrite

Fixation
Volatilized
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5th - 7th NOVEMBER

PRELIMINARY PROGRAM

FIACUI-LACQUA 2014

Hotel Presidente Intercontinental Guadalajara.

Guadalajara, Jalisco, Mexico.

Schedule subject to change without notice

LYNX HALL

AQUARIUM HALL

ANTARES HALL

PERSEUS HALL

SCORPIO HALL

ARIES HALL

Wednesday, November 5th

07:00 - 09:00
08:00 - 16:00
09:00 - 09:40
09:40 - 10:20
10:20 - 13:00

13:00 - 15:00
15:00 - 17:00

17:00 - 17:20
17:20 - 19:00

19:30 - 22:00
17:00 - 20:00

Registration & Inscriptions + Posters Assembly.

Tradeshow Assembly.
MASTER CONFERENCE: "Effects of Climate Change in Aquaculture", Adalberto Val - Director of the National Institute for Amazonian Research, INPA, Brazil.
Recess & Coffee break, Lobby.
Aquaculture of Indigenous
Sustainable Aquaculture.
Crustaceans Aquaculture.
Mollusks Aquaculture.
General Contributions to
Ecology & Limnology.
- Macrobrachium and other
Species.
- For a changing World.
-Reproduction.
Aquaculture.
crustaceans.
- Financial Integration of
- Genetics.
- Moderator: Patricia Valenti,
sustainable models.
CAUNESP, Brazil; Eduardo Ballester,
UFRG, Brazil; Fernando Villasante,
- Moderator: Sergio Guevara,
- Moderator: Carlos Martinez, - Moderator: Adalberto Val,
- Moderator: Helcio Marques,
CESAIBC, Mexico.
UdeG, Mexico.
INPA, Brazil.
UMSNH, Mexico.
APTA, Brazil.
Lunch.
Aquaculture Economics.
Education, Training & Technology
Mollusks Aquaculture.
Micro-Algae.
Aquaculture of Indigenous
Sustainable Aquaculture.
Transfer.
- Nutrition.
-Multi-trophic integrated
Species.
- Moderator: Newton Rodrigues,
- Moderator: Pilar Sanchez, - Moderator: Fco. Javier
- Grow-out.
aquaculture.
Martinez Cordero, CIAD, Mexico. Brazil.
CICESE, Mexico.
- Moderator: Luiz Poersch, Brazil.
Recess & Coffee break, Lobby.
Sustainable Aquaculture.
Mollusks Aquaculture.
Fish Larviculture.
Aquaculture Economics.
Aquaculture in LA&C.
Macro-Algae.
- Aquaponics.
- Environmental & Health
- Moderator: Wilfrido Contreras,
- Organic Aquaculture.
Managment.
UJAT, Mexico; Antonio Garza,
- Moderator: Ma. Celia Portella, - Moderator: Carlos Leon, BoFish, Mexico;
LACC-WAS. Mexico; Felipe
- Moderator: Pilar Sanchez,
Manuel Segovia, CICESE, Mexico.
CAUNESP, Brazil.
Matias, FAO, Brazil.
CICESE, Mexico.
Welcome Cocktail.
TRADESHOW SCHEDULE.

Thursday, November 6th

08:00 - 10:00
10:00 - 11:00
11:00 - 11:40
11:40 - 12:00
12:00 - 13:00

Registration & Inscriptions + Posters Assembly.

OFFICIAL INAUGURATION BY FEDERAL AND LOCAL AUTHORITIES.
MASTER CONFERENCE: "Strategies for the Development of Aquaculture in Mexico", TBA.
Recess & Coffee break, Tradeshow.
Tilapia Aquaculture.
Crustaceans Aquaculture.
Feeds & Additives.
Marine Finfish Aquaculture.
- Reproduction & Genetics.
- Nutrition.
- Reproduction & Genetics.
- Moderator: Lorenzo Juarez,
- Moderator: Wilfrido Contreras,
ANCPN, Mexico; Luis Andre
UJAT, Mexico; Kevin Fitzsimmons,
- Moderator: Teresa Viana,
Sampaio, UFRC, Brazil; Juan
U. of Arizona, USA.
UABC, Mexico.
Pablo Lazo, CICESE, Mexico.
13:00 - 15:00 Lunch.
Feeds & Additives.
Marine Finfish Aquaculture.
15:00 - 16:40 Tilapia Aquaculture.
Crustaceans Aquaculture.
- Larviculture.
- Integrated Multi-trophic
Aquaculture.
16:40 - 17:00 Recess & Coffee break, Tradeshow.
Crustaceans Aquaculture.
17:00 - 18:40 Tilapia Aquaculture.
- Health.

Feeds & Additives.

Marine Finfish Aquaculture.

- Nutrition.

- Moderator: Scott Horton,

Nutrimar, Mexico.

Fish Morphology & Physiology.

Production Systems.
- Biofloc.

- Moderator; Wilson Wasielesky,

UFRC, Brazil; Sergio Zimmermann, - Moderator: Roberto Mendoza,
Zimmermann AquaSolutions, Brazil. UANL, Mexico.
Production Systems.
- Biofloc.

Fish Morphology & Physiology.

Production Systems.
- Recirculation.
- Moderator: Marcelo Shei,
PREAMAR, Brazil; Michael
Schwarz, Virginia Tech, USA.

Gender Inclusion in Aquaculture.

- Moderator: Felipe Matias, FAO,
Brazil.

10:00 - 20:00 TRADESHOW SCHEDULE.

Friday, November 7th

09:00 - 11:00 Fish Feeding & Nutrition.

Crustaceans Aquaculture.
Health.

- Moderator: Juan Pablo Lazo, - Moderator: Scott Horton,

CICESE, Mexico.
Nutrimar, Mexico.
11:00 - 11:20 Recess & Coffee Break, Tradeshow.
Crustaceans Aquaculture.
11:20 - 13:00 Fish Feeding & Nutrition.
- Nutrition.

13:00 - 15:00 Lunch.

Crustaceans Aquaculture.
15:00 - 17:40 Use of Fish Meal & Fish Oil.
- Moderator: Juan Pablo Lazo, - Nutrition.
CICESE, Mexico; Albert Tacon,
Aquatic Farms, USA.
17:40 - 19:00 CLOSING EVENT, TRADESHOW.
10.00 - 20:00 TRADESHOW SCHEDULE.

@FIACUI

Fish Health.

Production Systems.
- Recirculation.
- Moderator: Marcelo Shei,
- Moderator: Francisco Arregui, PREAMAR, Brazil; Michael
S.P.-Bagre, Mexico.
Schwarz, Virginia Tech, USA.
Catfish, Carp & other
Freshwater Species.

Reproduction & Genetics.

Fish Health.

Catfish, Carp & other

Freshwater Species.

Ornamental Species.
Reproduction & Genetics.
- Moderator: Marcelo Shei,
PREAMAR, Brazil; Nuno Simes,
UNAM, Mexico; Serafin Almenara,
AZOO Mexico, Mexico.

Fish Health.

Salmon & Trout Aquaculture.

Ornamental Species.

- Moderator: Camilo Pohlenz,

BIOMAR, Costa Rica.

Career Seminary for Students.

- Moderator: Juan Pablo Lazo,
CICESE, Mexico; Ma. Celia
Portella, CAUNESP, Brazil.

- Moderator: Luis Hector

Hernandez, UNAM, Mexico.

Panorama Acuicola Magazine

Aquaculture Magazine

@PanoramAcuicola
@AquacultureMag

- Moderator: Ricardo Ribeiro,

UEM, Brazil; Roberto Solis,
Acuaplan, Mexico.

Panorama Acuicola Magazine

Aquaculture Magazine

BOOTH INFORMATION: Carolina Mrquez, servicioaclientes@globaldp.es | International Sales: Steve Reynolds, marketing@dpinternationalinc.com and Mario Stael at mario@marevent.com | General Information: Mario Stael, mario@marevent.com

www.fiacui.com | www.panoramaacuicola.com | www.was.org | www.marevent.com

Participating companies

Sponsors

Media sponsor

Gold Sponsor

Bronze Sponsor

Bronze Sponsor

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Reduction in farm density was element in over

outbreak in Chile (A. Alvial).

A regional professional network in aquaculture established in 2009, the AANs mission is to help aquaculturists and farmers operate a profitable and environmentally sound
business, and in doing so, provide the world affordable products at high quality in a sustainable manner.

WORLD AQUACULTURE 2015 WILL FOLLOW THE VERY SUCCESSFUL

FIRST KOREAN MEETING - WORLD AQUACULTURE 2008 IN BUSAN
World Aquaculture 2015 will be held in Jeju Island one of the leading flatfish production centers in the world.
The rest of the growing aquaculture industry in Korea and the Asian Pacific region will also be represented.
A major international trade show at WORLD AQUACULTURE 2015 is the place to learn about the latest in
aquaculture, see the newest technology presented by exhibitors from around the world.

Stay in touch with the program developments for WORLD

AQUACULTURE 2015 through our Web Page at: www.was.org

Manufectured by

Innovation Towards Sustainability

MixotrophicTM System