Sei sulla pagina 1di 2

PROTEIN STANDARDS

Precision Plus Protein™


WesternC™ Standards

n Protein bands are


visible during
Prestained Standards for Chemiluminescent Blot Detection
electrophoresis,
after blot transfer,
and during Chemiluminescent
A precise protein standard is a critical Membrane Image
chemiluminescent
detection component of every gel electrophoresis MW, kD

experiment. High-quality protein standards 250


n S
 harp bands give
accurate molecular
should generate sharp, distinct, and
weight estimation reproducible bands for easy and accurate 150
molecular weight determination.
n Integral Strep-tag
sequence allows Precision Plus Protein WesternC standards 100
chemiluminescent
combine the accuracy and convenience of 75
detection
prestained protein markers with integrated
n D
 ual color makes chemiluminescent detection to allow
band reference easy 50
visualization during the entire electrophoresis
n M
 igration pattern is and western blotting process. 37
identical to that of
other standards in Traditional protein standards incorporate
the Precision Plus natural proteins of predetermined molecular 25
Protein standards
weight for sizing. Bio-Rad’s Precision Plus 20
family
Protein standards use recombinant proteins
for accurate sizing, providing clear and 15

precise bands that are not achievable with


10
native proteins. The family of Precision Plus
Protein standards includes WesternC,
all blue, Kaleidoscope™, dual color, and
Precision Plus Protein WesternC standards.
unstained standards for various
experimental applications.
Recombinant Protein Bands Prestained for Easy Monitoring
The Precision Plus Protein standards family The 25, 50, and 75 kD bands in the WesternC
uses carefully engineered recombinant standards are stained pink for easy reference
proteins to generate precise molecular and blot orientation; all other bands are
weights. All Precision Plus Protein standards stained blue. These prestained bands are
contain ten highly pure recombinant protein clearly visible during electrophoresis and
bands of 10, 15, 20, 25, 37, 50, 75, 100, 150, show equal brightness when transferred onto
and 250 kD. a membrane. Prestained protein standards
make it easy to monitor electrophoresis
progress and transfer efficiency.
Chemiluminescence and Charge-Coupled
Device (CCD) Imaging
Precision Plus Protein WesternC standards contain
an integrated Strep-tag sequence on each band that
enables parallel detection on western blots using a
StrepTactin-horseradish peroxidase (HRP) conjugate.
Thus, molecular weight of sample proteins can be
estimated directly on western blots. The WesternC
standards are ideal for visualization of blotting transfer
and accurate molecular weight estimation directly from
film exposure or CCD images.
Because WesternC standards are part of the Precision
Plus Protein standards family, they have clean, sharp
bands, enabling generation of publication-quality bands
directly on the image.
Ordering Information
Catalog # Description
161-0376 Precision Plus Protein WesternC Standards,
250 µl, 50 applications
161-0385 Precision Plus Protein WesternC Pack,
50 applications of WesternC standards and 50
applications of StrepTactin-HRP
161-0375 Precision Plus Protein Kaleidoscope Standards,
500 µl, 50 applications
161-0374 Precision Plus Protein Dual Color Standards,
500 µl, 50 applications
161-0373 Precision Plus Protein All Blue Standards,
500 µl, 50 applications
161-0363 Precision Plus Protein Unstained Standards,
1 ml, 100 applications
161-0380  recision Protein™ StrepTactin-HRP Conjugate,
P
300 µl, 150 applications
161-0381 Precision Protein StrepTactin-HRP Conjugate,
125 µl, 50 applications
170-5070 Immun-Star ™ WesternC™ Chemiluminescent Kit,
includes 50 ml luminol/enhancer, 50 ml peroxide solution
170-5044 Immun-Star Goat Anti-Mouse (GAM)-HRP
Detection Kit, 500 ml
170-5045 Immun-Star Goat Anti-Rabbit (GAR)-HRP
Detection Kit, 500 ml

StrepTactin and Strep-tag are trademarks of Institut für Bioanalytik GmbH.


Strep-tag technology for western blot detection is covered by U.S. patent
5,506,121 and by UK patent 2,272,698. StrepTactin is covered by German
patent application P 19641876.3. Bio-Rad Laboratories, Inc. is licensed by
Institut für Bioanalytik GmbH to sell these products for research use only.

Bio-Rad
Laboratories, Inc.

Life Science Web site www.bio-rad.com USA 800 4BIORAD Australia 61 02 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 21 3237 9400
Canada 905 712 2771 China 86 21 6426 0808 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00 France 01 47 95 69 65
Group Germany 089 318 84 0 Greece 30 210 777 4396 Hong Kong 852 2789 3300 Hungary 36 1 455 8800 India 91 124 4029300 Israel 03 963 6050
Italy 39 02 216091 Japan 03 5811 6270 Korea 82 2 3473 4460 Mexico 52 555 488 7670 The Netherlands 0318 540666 New Zealand 0508 805 500
Norway 23 38 41 30 Poland 48 22 331 99 99 Portugal 351 21 472 7700 Russia 7 495 721 14 04 Singapore 65 6415 3188 South Africa 27 861 246 723
Spain 34 91 590 5200 Sweden 08 555 12700 Switzerland 061 717 95 55 Taiwan 886 2 2578 7189 United Kingdom 020 8328 2000

Bulletin 5561 US/EG Rev B 07-0890 1107 Sig 1106

Potrebbero piacerti anche