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Santos, G.1, Silverio, A.1, Soriano, R.1, Tan, J.1, Tequillo, H.1
University of Santo Tomas, Faculty of Pharmacy1
Group 8 3F Pharmacy
ABSTRACT
The experiment was performed to understand the process of shelf life determination. Acetylsalicylic acid, also
known as aspirin, is one of the most commonly used analgesics worldwide and was used for this experiment. From a
stock solution of salicylic acid, five standard solutions were prepared and their absorbance values were determined
using the UV-VIS spectrophotometer at 296 nm. The best fit line was determined using linear regression. A sample
solution containing aspirin dissolved in water was placed in an oven at 50C. The absorbance readings at 296 nm
were taken after 15, 30, 45, 60 and 90 minutes. From this data, the concentrations of aspirin remaining together with
its logarithmic equivalent were determined. Using linear regression, the best fit line was once again obtained, which
is essential for the computation of shelf-life.
INTRODUCTION
Shelf life is best defined as the time span
over which the quality of a product remains within
specifications. That is, it is the time period over
which the efficacy, safety, and esthetics of a product
can be assured. When the degradation of the essential
components cannot be adequately described by a rate
expression, shelf life cannot be easily estimated or
projected.
Estimation of product shelf-life is done by
two methods estimation from data obtained under
the same conditions as those that the final product is
expected to withstand and the estimation from tests
conducted under accelerated conditions. [4]
Half-life is the amount of time required for a
quantity to fall to half its value as measured at the
beginning of the time period. It is used to describe a
quantity undergoing exponential decay, and is
constant over the lifetime of the decaying quantity.
[8]
Aspirin, also known as acetylsalicylic acid,
is a derivative of salicylic acid. It is the most widely
used medicine in the world. It has the ability to
reduce fever (antipyretic), reduce pain (analgesic),
relieve swelling and pain associated with rheumatism
and arthritis (anti-inflammatory), enhance the
B. Procedure
1.
60 mins
x = log (conc. of ASA)
x = log (0.082723)
x = -1.0824
90 mins
x = log (conc. of ASA)
x = log (0.082374)
x = -1.0842
CONCLUSION
Shelf-life is the time required for 10% of the drug to
degrade and 90% of the drug to remain intact. The
computed value for aspirin at 25oC is 2 days, 4 hours
and 14 minutes. Half-life is the time required for 50%
of the drug to degrade and 50% of the drug to remain
intact. The computed value for aspirin at 25oC is 14
days, 8 hours and 47 minutes.
REFERENCES
[1] Bayquen, A., et. al. (2009). Synthesis of
Acetylsalicylic Acid (Aspirin). In Laboratory
Manual in Organic Chemistry (p. 141). Quezon City,
Philippines: C & E Publishing.
[2] https://pharmahub.org/resources/535/download/
ASA_Freshman_Lab_Handout.pdf
[3] http://en.wikipedia.org/wiki/Aspirin
[4] Yoshioka, S., et. Al (2000). Stability of Drugs and
Dosage Form. Springer Science and Business Media.
[5] http://en.wikipedia.org/wiki/Aspirin
[6] http://en.wikipedia.org/wiki/Ultraviolet%E2%
80%93visible_spectroscopy
[7] http://en.wikipedia.org/wiki/Arrhenius_equation
[8] http://en.wikipedia.org/wiki/Half-life