CULTURE MEDIUM IN 1000 mL SOLUTION

Eosin-Methylene Blue Agar (EMB) 35.96 g

MacConkey Agar with 0.15% Bile salts, CV,
and NaCl (MAC)
51.53 g
Hektoen Enteric Agar (HEA) 76.66 g
Xylose-Lysine Deoxycholate Agar (XLD) 56.68 g
Salmonella-Shigella Agar (SSA) 63.02 g
Bismuth Sulphite Agar (BSA) 52.33 g
(TCBS) 89.08 g
Mannitol Salt Agar (MSA) 111.02 g
(BA)
(CA)
(PEA) NOT YET PREPARED
*Unless mentioned otherwise, heating to dissolve is done through heating ONLY.
**Autoclave - Autoclave at 15 lb pressure (121 C) for 15 minutes.
FORMATION SPECIAL NOTES
Plated
Autoclave*; Avoid Overheating; Shake to oxidize
methylene blue, to restore its blue color, and suspend
flocculent precipitate; if inoculated on same day, it may
be used without autoclave sterilization
Plated
Heat to boiling with gentle swirling to dissolve agar
completely; Autoclave; Surface of medium should be dry
when inoculated
Plated DO NOT AUTOCLAVE
Plated
DO NOT AUTOCLAVE OR OVERHEAT; Not advisable to
prepare large volumes which will require prolonged
heating
Plated
DO NOT AUTOCLAVE OR OVERHEAT; Heat with frequent
agitation to dissolve medium completely; Overheating
may destroy selectivity of the medium
Plated
DO NOT STERILIZE IN AUTOCLAVE OR BY FRACTIONAL
STERILIZATION - overheating may destroy selectivity of
medium; Sensitivity of medium depends largely upon
uniform dispersion of precipitated bismuth sulphite in the
final gel, which should be dispersed before puring into
the sterile petri plates.
Plated DO NOT AUTOCLAVE
Plated
Autoclave; Contains 7.5% NaCl as one of its ingredients.
On repeated exposure to air and moisture, NaCl has
tendency to form lumps, therefore it is strongly
recommended that storage should be in tightly closed
containers in a dry place away from bright light
NOT YET PREPARED
*Unless mentioned otherwise, heating to dissolve is done through heating ONLY.
**Autoclave - Autoclave at 15 lb pressure (121 C) for 15 minutes.
FUNCTION COLOR
For differential isolation of gram-negative
enteric bacilli from clinical and nonclinical
specimen
RED-VIOLET
For selective isolation and differentiation of
coliform organisms and other enteric
organisms
MAGENTA PINK
For differential and selective isolation of
Salmonella and Shigella species from
enteric pathological specimen
MUDDY GREEN
For selective isolation and enumeration of
Salmonella typhi and other Salmonella sp.
ORANGE
A differential, selective medium for the
isolation of Salmonella and some Shigella
species f rom pathological specimen,
suspended food stuff, etc.
SALMON PINK
(VERY LIGHT PINK)
Recommended for the selective isolation of
Salmonella from feces, urine, sewage, and
other materials.
COLORLESS
Recommended for the selective isolation
and cultivation of Vibrio cholerae and other
enteropathogenic Vibrios causing food
poisoning.
BLUE-GREEN (DARK
TURQUOISE)
For selective isolation of pathogenic
Staphylococci from pharmaceutical
products in accordance with the microbial
limit testing by harmonized methodology of
USP/EP/BP/JP/IP (Medium 13)
NOT YET PREPARED