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Values of D-dimer test in the diagnostics of pulmonary embolism
Vildana Arnautovi-Torlak
, Belma Pojski
, Hasan utic
, Admir Rama
Department of Internal Diseases, Cantonal Hospital Zenica,
Clinic for Pulmonary Diseases, Podhrastovi, Sarajevo,
Clinic of Gynecol-
ogy and Obstetrics Sarajevo; Bosnia and Herzegovina
Corresponding author:
Vildana Torlak-Arnautovi
Department of Internal Diseases,
Cantonal Hospital Zenica
Crkvice, 72000 Zenica,
Bosnia and Herzegovina
Phone: +387 32 405 133;
Fax: +387 32 226 576;
Original submission:
06 January 2014;
Revised submission:
01 March 2014;
07 April 2014.
Aim To establish a value of D-dimer and compare fndings of ele-
vated and normal values with the golden standard, computed to-
mography (CT) of lungs in patients who had symptoms indicating
pulmonary thromboembolism (PTE) at admission.
Methods This retrospective/prospective study was conducted at
the Department of Internal Diseases of the Cantonal Hospital Ze-
nica, Bosnia and Herzegovina. A sample included 80 patients with
symptoms indicating PTE at the time of admission, D-dimers and
CT scan of thoracic organs were performed. The patients were di-
vided into two groups: 40 examinees with PTE confrmed by CT
scan and 40 patients (control group) whose PTE was not confr-
med by the CT scan.
Results Sensitivity of D-dimer according to statistical calculation
was 87.5%. Specifcity of D-dimer was 57.5%. The chance of a
patient to have PTE in case of elevated values of D-dimer was
3.58 higher than in patients with normal D-dimer values. The po-
sitive predicative value of D-dimer was 0.54, the negative predica-
tive value was 0.75. Test accuracy was 57.5%. Values of D-dimer
>0.83 using a ROC curve and present clinical symptoms of the
disease have indicated further diagnostic examination according
algorithm and a need for CT scan (of thoracic organs )
Conclusion D-dimer is important in the diagnostics of PTE, high
sensitivity and low specifcity have been proven. A positive D-di-
mer test indicates the presence of PTE. However, the test is not
reliable. In order to set a diagnosis it is necessary to visualize a
blood clot by computed tomography of lungs.
Key words: product fbrin, CT, Wels score
Med Glas (Zenica) 2014; 11(2):
Pulmonary thromboembolism (PTE) is a clinical
and pathophysiological condition occurring when
a blood clot originating from veins of systemic
circulation taken by bloodstream occludes one of
the branches of the pulmonary artery and prevents
blood to reach a part of lungs (1). Pulmonary
thromboembolism is a leading cause of illness
and mortality, and it can occur in relation to many
clinical conditions (1,2). Pulmonary thromboem-
bolism is on the third place according to prevalen-
ce after the ischemic heart disease and cerebro-
vascular disease with the incidence of 1-2% in the
general population and 12-20% in the population
of hospitalized patients (2-4). Around 60% of all
cases of vein thromboembolism are hospitalized
patients, with 5-10% of patients suffering from
pulmonary thromboembolism (2,5,6). Epidemio-
logical studies indicate that it is responsible for
over 50,000 death cases in the USA (3,7). Howe-
ver, the available data show that less than 10%
of all cases of pulmonary embolism end up with
death (1,2,8,9). Therefore, the prevalence of fatal
embolism cases together with non-fatal embolism
is probably higher than 500,000 annually (2,3,5).
Researchers from the Duke University have fo-
und that the use of automatic, immunoturbidime-
tric D-dimer assay, in combination with clinical
algorithm of risk, favors the the assay as the frst
line in the treatment of patients with a high risk
of pulmonary embolism due to a small number of
samples available for the analysis from that po-
pulation (1,3,5). In clinical practice, the biggest
problem arises from the question which is the va-
lue of D-dimer assay as a marker for further dia-
gnostic algorithm with clinical suspicion of PTE
(10,12). The aim of the research was to compare
the elevated and normal D dimer test in patients
who had clinical suspicion of PTE at admission to
hospital and compare the value of D- dimer with
the golden standard, CT scan of thoracic organs.
Obtained data will help clinicians use the diagno-
stic algorithm and ensure timely treatment of pul-
monary embolism and prevention of death (8,13).
The clinical retrospective/prospective study was
conducted in the periods February-August 2012,
and September 2012 -March 2013, respectively,
at the Department of Internal Diseases, Cantonal
Hospital Zenica, Bosnia and Herzegovina. The
research was approved by the Ethics Committee
of the School of Medicine in Sarajevo and the
Director of the Cantonal Hospital Zenica. The
patients in the prospective study were informed
about the vital importance of conducting the dia-
gnostic algorithm and timely treatment, for whi-
ch they gave their consents. The sample included
80 patients with symptoms indicating probable
presence of PTE, and consequently D-dimers and
computed tomography (CT) of thoracic organs
were performed. All the patients were divided
into two groups: control group (n=40) included
patients with symptoms indicating the presence
of PE, who underwent complete diagnostic pro-
cedure, and the CT scan excluded pulmonary
thromboembolism. The study group (n=40) in-
volved patients with symptoms indicating PTE,
who underwent compete diagnostic procedure
and the CT scan confrmed PTE diagnosis.
The research examined the following parameters
taken at the onset of the disease (risk factors): tra-
uma, fractures (especially large bone of lower lim-
bs), orthopedic surgery (especially hip and knee),
major abdominal, gynecological, and vein surgery,
cardiovascular diseases accompanied by cardiac
decompensation and arrhythmias, septic conditi-
ons, malignant diseases, miscarriage, childbirth.
D-dimers were determined by a new method of
immunoturbidimetry (BCSX System, hemostasis
testing, Siemens Healthcare Diagnostic, Erlan-
gen, Germany) and interpreted as elevated above
the reference values (<500 ng/L) in the Biochemi-
cal Laboratory of the Cantonal Hospital Zenica.
Computed tomography was performed (Si-
emens Somatom defnition 44 slice device,
Erlangen,Germany) immediately after the admi-
ssion to hospital. The Ultravist 300 mg/ml pack
iopromide (Bayer Pharma AG, Berlin,Germany)
radiological contrast agent was used. Interpre-
tation of the scan was done by an experienced
radiologist of the Cantonal Hospital Zenica.
Nominal and ordinal variables were analyzed
using the
test, and in case of a lack of expected
frequency, Fisher exact test was applied. For con-
tinuous variables symmetry of their distribution
was frst analyzed with the Kolmogorov-Smirnov
test. When the distribution of continuous varia-
bles was symmetric, arithmetic mean and stan-
dard deviation were used to show mean values
Arnautovi-Torlak et al. D-dimer and pulmonary embolism
Medicinski Glasnik, Volume 11, Number 2, July 2014
and dispersion measure. To compare variables
parametric tests were applied (Students t test and
ANOVA). Signifcance level was at p<0.05.
The average age of the control group and the
study group was 61.0714.44 (22-84) and
58.6016.40 years (21-80), respectively. Average
age of the patients involved in the research was
59.8315.40 years (21-84) (p=0.476) (Figure 1).
The analysis of average values of coagulation
factors in the study and control group has shown
signifcantly elevated levels of D-dimer in the
study group (p=0.002). In the analysis of the ave-
rage values of D-dimer test, we found that the
value of D dimer was statistically higher in the
study group patients (p = 0.002). The values of
D-dimer varied from physiological values and
they were signifcantly higher in the study gro-
up (p=0.002). Average values of activated partial
thromboplastin time (APTT) and international
normalized ratio (INR) were not statistically dif-
ferent (Figure 2).
Cough 25 (62.5) 23 (57.5) 0.803
Pain 29 (72.5) 36 (90.0) 0.045
Diffcult breathing and suffocation 29 (72.5) 29 (72.5) 1
Table 1. Prevalence of clinical symptoms in patients with
clinical suspicion of pulmonary thromboembolism
No of patients
Parameter Study group Control group
Increased D-dimer values 37 (92.5%) 31 (77.5%)
Within normal physiological values
3 (7.5%) 9 (22.5%)
Table 2. Prevalence of physiological and pathological values
of D-dimer within the examined groups
Figure 1. Age of patients as compared with the control group
Figure 2. Average values of coagulation factors in the study
and control groups; APTT, activated partial thromboplastin time;
INR, international normalised ratio;
The analysis of symptoms indicating pulmonary
thromboembolism has shown that 25 (62.5%)
patients from the control group and 23 (57.5%)
from the examined group were coughing, equal
percentage of the examinees in both groups, 29
(72.5%), had diffculties breathing and suffo-
cation; however, when it comes to the presence
of pain, 36 (90%) patients from the study group
experienced pain as compared with 29 (72.5%)
from the control group (p=0.045) (Table 1).
Analyzing the values of coagulation factors in
the control and study group we found that the ele-
vated D-dimer was more present in the patients
from the study group (n=37), as compared with
the examinees from the control group; there is a
statistically signifcant difference in the prevalen-
ce of pathological fndings of D-dimer between
the groups (p=0.047) (Table 2).
The patients had 76.2% higher chance of ge-
tting PTE if the values of D-dimer were hi-
gher than 0.83 (p<0.05; cut off >0.83; CI95%
0.653 0.850). The sensitivity of D-dimer of
87.5% (CI95% 73.2 95.8), and increased va-
lues of D-dimer obtained by the immunoturbidi-
metry method occurred in 87.5% patients with
the symptoms of PTE, whose diagnosis was con-
frmed by the CT scan. D-dimer specifcity was
57.5% (CI 95% 40.973.0), e.g. 57.5% patients
D-dimer obtained by immunoturbidimetry was
increased with no presence of pulmonary throm-
boembolism (it was not proven by the CT scan)
(Figure 3).
Determining D-dimer concentration is one of the
basic tests used for exclusion of the diagnosis of
thromboembolic diseases, deep vein thrombosis
and pulmonary embolism (11-13). Testing of pla-
smatic D-dimers in the patients in this research
admitted to the Department for Internal Diseases,
the Cantonal Hospital Zenica, proved to be an
excellent non-invasive triage test in patients with
suspected PTE (10,14,15). The age difference of
both studied groups was not statistically signif-
cant. Average age in this study was 59.83 years.
A number of studies showed that the incidence
of PTE is higher in the middle and old age, and
signifcantly higher in patients over 45 (7,11). In
our study we analyzed the symptoms that indi-
cated pulmonary thromboembolism and it was
found that 62.5% of the patients of the control
group and 57.5% of the test group had had cough,
equal percentage of respondents in both groups
(72.5%) had experienced wheezing and choking,
and 90% and 72.5% of the patients from the
studied and control group, respectively, had felt
pain. D -dimer has a signifcant place in the di-
agnostic algorithm of the acute PTE (6,16,). Our
results showed high sensitivity of D-dimer test.
D-dimer test is sensitive, but it is not a speci-
fc marker for pulmonary thromboembolism
(13,17). It has a low level of false negative re-
sults (15,18). Our results showed that from the
study group only 3.75% of patients had a nega-
tive value of D-dimer and the risk of pulmonary
thromboembolism was high, which is the reason
why CT of thoracic organs was performed thus
proving PTE. The negative value of D dimer test
in combination with low risk by Wells score can
effectively eliminate the need for expensive di-
agnostic imaging methods, which is confrmed
by the references (13,15). Researchers at Duke
University have found that the use of automa-
tic immunoturbidimetric D-dimer test is useful
in combination with certain risk factors through
Walls, as the frst-line screening test for diagno-
sing pulmonary thromboembolism (19,20).
This study suggests that a negative D-dimer test
with a little pretest is likely exclude the presence
of venous thromboembolism, which was shown
by Bates and his associates (7).
Based on the obtained values of D-dimer test in
the present study, the negative predicative value
of D-dimer was 0.75, which means that in case
patients had physiological values of D-dimer
with unclear clinical presentation of PTE, the
probability that they had no PTE was 75%.
Carrier et al. discovered that among the patients
aged 60-80, a low to negative Wells score in com-
bination with a negative D-dimer test occurred in
99% of the negative predictive value (NPV), in-
dicating the probability that patients have pulmo-
nary thromboembolism is 99%. (7,10). However,
in patients older than 80 with low negative Wells
score in combination with the negative D-dimer
test, 21-31% resulted in a negative predicative
value (15). A study by Wels, Carries et al, et al.
indicated that the use of a manual D-dimer test
of blood agglutination in combination with the
grading system for clinical pretest probability
known as the Wells score resulted in the signi-
fcant decrease of number of patients who had
undergone the diagnostic method of compression
ultrasound (7,20).
D- dimer test is not a specifc marker for PTE,
however, it serves as an accessory test in the di-
agnostic protocol for the exclusion of pulmonary
thromboembolism. In our study a positive predi-
cative value of the D-dimer implied that the CT
confrmed PTE in 54% of patients corresponding
to the results in the reviewed literature (11,21).
Many factors are associated with positive D di-
mer (16). D-dimer test can be elevated in a range
of non-thrombolytic disorders including recent
surgeries, bleeding, injuries, pregnancy, postpar-
tum period, age older than 80 years, malignity
Figure 3. Analysis of ROC curve D-dimer values
Arnautovi-Torlak et al. D-dimer and pulmonary embolism
Medicinski Glasnik, Volume 11, Number 2, July 2014
and septic condition (7,15). In clinical practice
the biggest problem arises from the question whi-
ch value of D-dimer test is to serve as a marker
for further diagnostic algorithm in case of clini-
cal suspicion of PTE (17). Application of cut-off
values signifcantly increases specifcity without
changing the sensitivity of the test thereby im-
proving clinical beneft of D-dimer in the diagno-
sis of PTE (10,22). In this study we found that in
case of clinical suspicion of pulmonary thrombo-
embolism, and D-dimer values higher than 0.83,
further diagnostic algorithm needed to include
CT thoracic organs (23). Based on AUC ROC
curve, we can conclude that respondents have
76.2% greater risk of developing PTE if they
have D-dimer values higher than 0.83. Increased
limits on the values of D-dimer can reduce false
positivity, and increase the false-negative results
in older patients (11,22,23).
In conclusion, we found that D-dimer test was a
valuable tool in the evaluation of patients with
suspected PTE, and it may help physicians with
further diagnostic algorithm and eliminate the
need for radiological tests, but only in combinati-
on with clinical presentation, associated risk fac-
tors and the possible presence of other diseases,
which can give similar symptoms of pulmonary
The authors would like to thank colleagues from
the Cantonal Hospital Zenica, the Department of
Internal Medicine, experienced radiologists and
Sadida Zecevic, MSc, for accurate laboratory di-
No specifc funding was received for this study.
Competing interests: none to declare.
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Vrijednost D-dimer testa u dijagnostici akutne plune tromboembolije
Vildana Torlak-Arnautovi
, Belma Pojski
, Hasan uti

Admir Rama
Sluba za unutranje bolesti, Kantonalna bolnica Zenica;
Klinika za plune bolesti i tbc Podhrastovi Sarajevo;
Klinika za ginekologiju
i akuerstvo Sarajevo; Bosna i Hercegovina
Cilj istraivanja je utvrditi vrijednost D-dimera i uporediti nalaz povienih i normalnih vrijednosti sa
zlatnim standardom kompjuterizovane tomografje plua (CT) kod pacijenata koji su pri prijemu
imali simptome koji ukazuju na plunu tromboemboliju (PTE).
Metode U retrospektivno-prospektivnoj studiji, provedenoj na Internom odjelu Kantonalne bolnice
Zenica, uzorak je sainjavalo 80 bolesnika sa simptomima koji su, pri prijemu u bolnicu, ukazivali na
moguu PTE. Odreena je vrijednost D-dimera i uraen CT torakalnih organa. Pacijenti su podijeljeni u
dvije grupe: 40 ispitanika kojima je prema CT-u dokazana PTE i 40 ispitanika kojima to nije dokazano
(kontrolna grupa).
Rezultati Osjetljivost D-dimer testa iznosila je 87.5%, a specifnost 57.5%. Izraunavanjem omjera
izgleda ustanovljeno je da je ansa da pacijent ima PTE kod povienih vrijednosti D-dimera 3,58 puta
bila vea nego kod ispitanika koji su imali normalne vrijednosti D-dimera. Pozitivna prediktivna vrijed-
nost D-dimera iznosila je 0.54, a negativna 0.75. Tanost testa iznosila je 57,5%. Vrijednosti D-dimera
>0,83 primjenom ROC krive i prisutni kliniki simptomi bolesti, ukazuju da se u daljem dijagnostikom
algoritmu obavezno uradi CT torakalnih organa.
Zakljuak Odreivanje vrijednosti D-dimer testa u dijagnostici PTE-a je znaajna. Pozitivan D-dimer
ukazuje na plunu emboliju, ali test nije pouzdan. Za postavljanje dijagnoze PTE-a potrebna je vizuali-
zacija tromba putem kompjuterizovane tomografje plua.
Kljune rijei: produkt fbrina, CT, Wels-scor
Arnautovi-Torlak et al. D-dimer and pulmonary embolism