VIS

O000ra10101L-200
8011
Edited by
Marine Bioprocess
Engineering
Proceedings of an International Symposium organized under auspices of
The Working Party an Applied Biocatalysis of the Eurpean Federation of Biotechnology
and The European Society for Marine Biotechnology,
Noordwijkerhout, The Netherlands, November 8-11, 1998
R. Osinga
Food and Bioprocess Engineering Group, Wageningen Agricultural University,
Wageningen, The Netherlands
J. Tramper
Food and Bioprocess Engineering group, Wageningen Agricultural University,
Wageningen, The Netherlands
J. G. Burgess
Department of Biological Science, Heriott-Watt University,
Edinburgh, United Kingdom
R.H. Wijffels
Food and Bioprocess Engineering group, Wageningen Agricultural University,
Wageningen, The Netherlands
progress in industrial microbiology
1999
ELSEVIER
Amsterdam - Lausanne - New York - Oxford - Shannon - Singapore - Tokyo
Contents
Cited in: Biological Abstracts; Biological & Agricultural Index; Cambridge Scientific Abstracts; Chemical Abstracts;
Elsevier BIOBASE/Current Awareness in Biological Sciences; Current Contents (Agriculture, Biology & Environmental
Sciences); EMBASE/Excerpta Medica; Index Medicus
Special lssue
Marine Bioprocess Engineering
Editorial

1
Opening keynote
S.A. Pomponi (USA)
The bioprocess-technological potential of the sea

5
Products
M.H.G. Munro, J.W. Blunt, E.J. Dumdei, S.J.H. Hickford, R.E. Lill, S. Li, C.N. Battershill and A.R. Duckworth
(New Zealand)
The discovery and development of marine compounds with pharmaceutical potential 1 5
J.G. Burgess, E.M. Jordan, M. Bregu, A. Mearns-Spragg and K.G. Boyd (UK)
Microbial antagonism: a neglected avenue of natural products research 27
T. Matsunaga, H. Takeyama, T. Nakao and A. Yamazawa (Japan)
Screening of marine microalgae for bioremediation of cadmium-polluted seawater 33
E.G. Vrieling, T.P.M. Beelen, R.A. van Santen and W.W.C. Gieskes (The Netherlands)
Diatom silicon biomineralization as an inspirational source of new approaches to silica production 39
M. Turkiewicz, E. Gromek, H. Kalinowska and M. Zielitiska (Poland)
Biosynthesis and properties of an extracellular metalloprotease from the Antarctic marine bacterium
Sphingomonas paucimobilis 53
S. Kreitlow, S. Mundt and U. Lindequist (Germany)
Cyanobacteriaa potential source of new biologically active substances 6 1
F. Sponga, L. Cavaletti, A. Lazzarini, A. Borghi, Ciciliato, D. Losi and F. Marinelli (Italy)
Biodiversity and potentials of marine-derived microorganisms 6 5
Csgr, D. Melgar, K. Schmidt and C. Posten (Germany)
Production and particle characterization of the frustules of Cyclotella cryptica in comparison with siliceous earth 71
G. Liebezeit, T.D. Knnemann and G. Gad (Germany)
Biotechnological potential of North Sea satt marsh plantsa review of traditional knowledge 77
A. Koulman, L.M.C. Pruijn, T.S.A. Sandstra, H.J. Woerdenbag and N. Pras (The Netherlands)
The pharmaceutical exploration of cold water ascidians from the Netherlands: a possible source of new
cytotoxic natural products

85
Energy
J. Miyake, M. Miyake and Y. Asada (Japan)
Biotechnological hydrogen production: research for efficient light energy conversion

89
1. Eroglu, K. Aslan, U. Gndz, M. Ycel and L. Trker (Turkey)
Substrate consumption rates for hydrogen production by Rhodobacter sphaeroides in a column photo-
bioreactor

1 03
V. Sediroglu, 1. Eroglu, M. Ycel, L. Trker and U. Gndz (Turkey)
The biocatalytic effect of Halobacterium halobium on photoelectrochemical hydrogen production

1 1 5
D.O. Yet, U. Gndz, L. Trker, M. Ycel and 1. Eroglu (Turkey)
Identification of by-products in hydrogen producing bacteria; Rhodobacter sphaeroides O.U. 001 grown in
the walte water of a sugar refinery

1 25
Cultivation of marine organisms
B. Rinkevich (Israel)
Cell cultures from marine invertebrates: obstacles, new approaches and recent improvements 1 33
R. Osinga, P.B. de Beukelaer, E.M. Melier, J. Tramper and R.H. Wijffels (The Netherlands)
Growth of the sponge Pseudosuberites (aff.) andrewsi in a closed system 1 55
E. Armstrong, J.D. McKenzie and G.T. Goldsworthy (UK)
Aquaculture of sponges on scallops for natura) products research and antifouling 1 6 3
B.J.B. Wood, P.H.K. Grimson, J.B. German and M. Turner (UK, USA)
Photoheterotrophy in the production of phytoplankton organisms 1 75
M.E. de Swaaf, T.C. de Re, G. Eggink and L. Sijtsma (The Netherlands)
Optimisation of docosahexaenoic acid production in batch cultivations by Crypthecodinium cohnii 1 85
R.D. Bowles, A.E. Hunt, G.B. Bremer, M.G. Duchars and R.A. Eaton (UK)
Long-chain n-3 polyunsaturated fatty acid production by members of the marine protistan group the
thraustochytrids: screening of isolates and optimisation of docosahexaenoic acid production 1 93
H. Helmholz, P. Etoundi and U. Lindequist (Germany)
Cultivation of the marine basidiomycete Nia vibrissa (Moore & Meyers) 203
S. La Barre, S. Singer, E. Erard-Le Denn and M. Jozefowicz (France)
Controlled cultivation of Alexandrium minutum and [33P1 orthophosphate cell labeling towards surface
adhesion tests 207
J.C. Ogbonna, S. Tomiyama and 1-1. Tanaka (Japan)
Production of a-tocopherol by sequential heterotrophicphotoautotrophic cultivation of Euglena gracilis 21 3
K. Tsukahara, S. Sawayama, T. Yagishita and T. Ogi (Japan)
Effect of Ca2+ channel blockers on glycerol levels in Dunaliella tertiolecta under hypoosmotic stress 223
P. Garcia-Jimenez, F.D. Marian, M. Rodrigo and R.R. Robaina (Spain)
Sporulation and sterilization method for axenic culture of Gelidium canariensis 227
Design and scale-up of in vitro cultures
E. Molina Grima, F.G.A. Fernndez, F. Garcia Camacho and
Y. Chisti (Spain)
Photobioreactors: light regime, mass transfer, and scaleup 231
A. Snchez Mirn, A. Contreras Gmez, F. Garcia Camacho, E. Molina Grima and
Y. Chisti (Spain)
Comparative evaluation of compact photobioreactors for large-scale monoculture of microalgae

249
M.M. Rebolloso Fuentes, J.L. Garcia Snchez, J.M. Fernndez Sevilla, F.G. Acidn Fernndez, J.A. Snchez
Pdrez and E. Molina Grima (Spain)
Outdoor continuous culture of Porphyridium cruentum in a tubular photobioreactor: quantitative analysis of
the daily cyclic variation of culture parameters
271
J.C. Ogbonna, T. Soejima and H. Tanaka (Japan)
An Integrated solar and artificial light system for internal illumination of photobioreactors 289
G. Chini Zittelli, F. Lavista, A. Bastianini, L. Rodolfi, M. Vincenzini and M.R. Tredici (Italy)
Production of eicosapentaenoic acid by Nannochloropsis sp. cultures in outdoor tubular photobioreactors 299
M.A. Borowitzka (Australia)
Commercial production of microalgae: ponds, tanks, tubes and fermenters 31 3
M. Janssen, T.C. Kuijpers, B. Veldhoen, M.B. Ternbach, J. Tramper, L.R. Mur and R.H. Wefels (Netherlands)
Specific growth rate of Chlamydomonas reinhardtii and Chlorella sorokiniana under medium duration
light/dark cycles: 1 3-87 s
323
D. Baquerisse, S. Nouals, A. lsambert, P.F. dos Santos and G. Durand (France)
Modelling of a continuous pilot photobioreactor for microalgae production 335
P.C. Wright, C. Stevenson, E. McEvoy and J.G. Burgess (UK)
Opportunities for marine bioprocess intensification using novel bioreactor design: frequency of barotoler-
ance in microorganisms obtained from surface waters
343
N. Zou and A. Richmond (Israel)
Effect of light-path length in outdoor fiat plate reactors an output rate of cell mass and of EPA in
Nannochloropsis sp. 351
K. Kotzabasis, A. Hatziathanasiou, M.V. Bengoa-Ruigomez, M. Kentouri and P. Divanach (Greece, Spain)
Methanol as alternative carbon source for quicker efficient production of the microalgae Chlorella minutis-
sima: Role of the concentration and frequente of administration 357
Product recovery
L.A.M. van der Wielen and L.K. Cabatingan (The Netherlands, Philippines)
Fishing products from the searational downstream processing of marine bioproducts 36 3
S. Hirano, T. Nakahira, M. Nakagawa and S.K. Kim (Japan, South Korea)
The preparation and applications of functional fibres from trab shell chitin 373
A. Robles Medina, L. Esteban Cerdn, A. Gimdnez Gim6nez, B. Camacho Pez, M.J. Ibnez Gonzlez and E.
Molina Grima (Spain)
Lipase-catalyzed esterification of glycerol and polyunsaturated fatty acids from fish and microalgae oils 379
L. Vandanjon, P. Jaouen, N. Rossignol, F. Oudmdneur and J.-M. Robert (France)
Concentration and desalting by membrane processes of a natural pigment produced by the marine diatom
Haslea ostrearia Simonsen

393
Closing keynote
O.R. Zaborsky (USA)
Marine bioprocess engineering: the missing link to commercialization

403
Author Index 409
Subject Index 41 3