8/17/2014 First test microscope PZO, DIC Pluta

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Invisible Life " INVISIBLE LIFE. FORUM COM INSTRUMENTAL and DIY Forum First test microscope PZO, DIC Pluta
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First test microscope PZO, DIC
Pluta
on: July 31, 2013, 20:03:43 pm
francisco
Hero
Posts: 752
Reputation 5
Hi
I purchased a PZO microscope equipped with DIC Pluta.
's first impression is quite good, it is a sturdy and good mechanical precision
microscope.
A level on Dec optical system seems interesting and a value very favorable. I
guess the most modern systems in December; Olympus, Nikon, Zeiss ....
proporcinaran more accurate results that this system PZO.
microscope came without me lighting system which, provisionally, I improvised
a pvc pipe with 1W Led waiting to find some enlightening for adaptation.
The binocular head is apparently very few trinocular heads that were
manufactured for these microscopes so to make the video have replaced the
head by the cctv camera. It is a heavy and unpleasant task looking specimen
through a field of view as small as that provided by the camera.
The head diameter is 42mm I think the same as the Olympus BH Is it okay ?.
Buscare some head to adapt and have vision and puero to camera. Do you
know of any trinocular head (besides the Olympus) having this measure ?.
DIC My experience is nil so any suggestions, criticism, help and information will
be received with great interest and appreciation.
What is the difference between the different prisms 10X, 20X, 40X
(condenser)?
Thank
Francisco http://youtu.be/16CuJYcNhdA
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Re: First test PZO microscope, DIC
RogelioMoreno
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Pluta
Reply # 1 on: July 31, 2013,
23:27:06 pm
Hero
Posts: 928
Reputation 2
Sex:
Francisco, Very interesting, congratulations on your new DEC computer. With
practice you'll master the best settings to get the results you want. Prisms
capacitor (10x, 20x, 40x, etc) are to be used for objectives with the same
increases as specified in the prism, ie one objective should be used with 10x
10x objective and so on. , you're right in terms of diameter heads and Olympus
BH BH2 are 42mm. I'm not sure but I think Nikon Optiphot / 43mm or 42mm is
Labophot. have to ask a seller on ebay. Regarding configuration DIC: if you
use lenses designed for that microscope then I do not think you need to move
the prism goes up goals (I have understood that the Dec PZO Fits any such
objectives and allows the prism goes above objectives can move up / down to
locate the prism in the back focal plane of the objective (to get the best DIC)).
I would see pictures of the DIC system espcificamente area of the prism is
above objectives. Greetings, Rogelio
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Re: First test PZO microscope, DIC
Pluta
Reply # 2 on: July 31, 2013, 23:29:45 pm

Hugo
Hero
Posts: 815
Reputation 17
Sex:
Hi Francisco. I really like this goes DIC, very good resolution. The polarizer
makes the funds are colored ("tint plate" or quarter-lambda if I remember
correctly) is fixed or can be removed?
A priori, without taking action, could be a Lomo or Carl Zeiss Jena head works
for you, East microscopes were quite compatible Were you thinking of a head
BH2?
Zeiss West 43,5mm measures
42mm Olympus BH2 Regards Hugo
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Re: First test PZO microscope, DIC
Pluta
Reply # 3 on: July 31, 2013, 23:37:33 pm

Hugo
Hero
From this comes the name ... Maksymilian Pluta
http://micro.magnet.fsu.edu/optics/timeline/people/pluta.html Hugo
8/17/2014 First test microscope PZO, DIC Pluta
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Posts: 815
Reputation 17
Sex:
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Re: First test PZO microscope, DIC
Pluta
Reply # 4 on: July 31, 2013, 23:48:02 pm

Jose Ricardo
User Jr
Posts: 67
Reputation 0
. Hola Francisco
Congratulations on your purchase. I do not know the quality of the most
modern in December and I have no possibility to compare. What if I think is
that the optics have come a long way and the level of the current goals is very
high. Just look at the "Chinese" objectives that offer very nearby images to
the middle range of Olympus and Nikon a few years ago, for a fraction of the
cost. Regarding the quality of this December, I can say I've seen pictures in
forums that used hybrids thereof and the result is impressive. equipment is
easy to use (at least if you limit yourself to December, which is what I have).
The imaging requires lots of light, 1W not have much. You can easily contruirte
an aluminum cylinder with a powerful LED on the end (no information on this.)
I have a box of 100w halogen lamps and the 40x objective notice that I need
light. Regarding the head, I use a trinocular the BH2. It is a little loose and
therefore misaligned with the optical axis (although this is fixable with a bit of
DIY). Finally, with respect to the difference of the prisms, do not quite
understand what you're asking. But you commented elocubracin a mine on
them: because there prisms for each of the increases (causing diffractions on
demand for each of them) and one common to reassemble the scattered light,
I understand that the result of the prism assembly homologous target and the
image size / diffraction corrected for the common prism. I'll be happy if I can
answer to the extent of my limited knowledge, to some concerns over this
element. Glad there is another forero with this team and to share knowledge
and experience. Greetings. R. Jose
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Re: First test PZO microscope, DIC
Pluta
Hugo
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Reply # 5 on: August 1, 2013, 00:01:02
am
Hero
Posts: 815
Reputation 17
Sex:
If the head is loose then BH2 should be tailored to the Lomo Zeiss and they
are bigger 1.5mm. And better too because there are many of these. Hugo
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Re: First test PZO microscope, DIC
Pluta
Reply # 6 on: August 1, 2013, 00:22:45
am
francisco
Hero
Posts: 752
Reputation 5
Quote: Ricardo Jose on July 31, 2013, 23:48:02 pm
. Hola Francisco
Congratulations on your purchase. I do not know the quality of the most modern in
December and I have no possibility to compare. What if I think is that the optics
have come a long way and the level of the current goals is very high. Just look at
the "Chinese" objectives that offer very nearby images to the middle range of
Olympus and Nikon a few years ago, for a fraction of the cost. Regarding the quality
of this December, I can say I've seen pictures in forums that used hybrids thereof
and the result is impressive. equipment is easy to use (at least if you limit yourself
to December, which is what I have). The imaging requires lots of light, 1W not have
much. You can easily contruirte an aluminum cylinder with a powerful LED on the
end (no information on this.) I have a box of 100w halogen lamps and the 40x
objective notice that I need light. Regarding the head, I use a trinocular the BH2. It
is a little loose and therefore misaligned with the optical axis (although this is
fixable with a bit of DIY). Finally, with respect to the difference of the prisms, do not
quite understand what you're asking. But you commented elocubracin a mine on
them: because there prisms for each of the increases (causing diffractions on
demand for each of them) and one common to reassemble the scattered light, I
understand that the result of the prism assembly homologous target and the image
size / diffraction corrected for the common prism. I'll be happy if I can answer to
the extent of my limited knowledge, to some concerns over this element. Glad
there is another forero with this team and to share knowledge and experience.
Greetings. R. Jose
Jose Ricardo Hi
Thanks for the reply and offer. Your knowledge and experience in this system
will be of great help.
Yeah, 1W is poor lighting. I have received a specific illuminator for these
microscopes, in this case the PZO Studar tomorrow for themselves to adapt. I
hope I have the problem that the field of view of the eyepiece is not full
8/17/2014 First test microscope PZO, DIC Pluta
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disappear even think this will be because the previous owner has tampered
with the internal lenses of the lighting system.
Personally despite the targets used in video "standard "(The 20X is a phase
contrast Nikon because I do not have another 20X) the outcome seemed
pretty interesting.
Greetings
Francisco
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Re: First test PZO microscope, DIC
Pluta
Reply # 7 on: August 1, 2013, 00:37:29
am
francisco
Hero
Posts: 752
Reputation 5
Quote: RogelioMoreno on July 31, 2013, 23:27:06 pm
Francisco, Very interesting, congratulations on your new DEC computer. With practice
you'll master the best settings to get the results you want. Prisms capacitor (10x,
20x, 40x, etc) are to be used for objectives with the same increases as specified in
the prism, ie one objective should be used with 10x 10x objective and so on. ,
you're right in terms of diameter heads and Olympus BH BH2 are 42mm. I'm not
sure but I think Nikon Optiphot / 43mm or 42mm is Labophot. have to ask a seller
on ebay. Regarding configuration DIC: if you use lenses designed for that
microscope then I do not think you need to move the prism goes up goals (I have
understood that the Dec PZO Fits any such objectives and allows the prism goes
above objectives can move up / down to locate the prism in the back focal plane of
the objective (to get the best DIC)). I would see pictures of the DIC system
espcificamente area of the prism is above objectives. Greetings, Rogelio
Hi Rogelio
Regarding the prisms was asking because apparently any combination of
condenser and objective prism gives me the same effect, ie ejmplo; condenser
prism 10X or 10X objective and 20X or 40X offer me the same effect, same
with condenser prism 20X or 40X and 10X objective ..... I repeat it yet
apparently not well know the DIC sitemas.
I think This system is used for both DIC (position 1 of the "head") and also
interference microscope (positions 2 and 3 of "head"). The particular aims of
this system are used for the band system for determining the refractive index
,. thickness of the sample ... etc but they are not valid for "normal" image for
only double or so separate images are observed.
Tomorrow make a few photographs for publication system
Regards
Francisco
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Re: First test PZO microscope, DIC
Pluta
Reply # 8 on: August 1, 2013, 00:50:17
am
francisco
Hero
Posts: 752
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Reputation 5
Quote: Hugo on July 31, 2013, 23:29:45 pm
Hi Francisco. I really like this goes DIC, very good resolution. The polarizer makes
the funds are colored ("tint plate" or quarter-lambda if I remember correctly) is
fixed or can be removed?
A priori, without taking action, could be a Lomo or Carl Zeiss Jena head works for
you, East microscopes were quite compatible Were you thinking of a head BH2?
Zeiss West 43,5mm measures
42mm Olympus BH2 Regards Hugo
Hi Hugo
Thanks for your comment and information
I think is a very attractive system for performance and price
The system uses two polarizers, the head which is movable and condenser
which is movable and removable since it also is used in the condenser variable
phase contrast PZO (I think unique to this brand) that allows a single capacitor
with the same goals and contrast both positive and negative phases. The
system is based on darken polarizer by rotating either of the two rings has the
PHZ phase goals. Simple and very interesting system.
tried the Zeiss photomicroscope my head and fits well but this kind of heads
are not in trinocular version as the port for the camera is built into the
Optovar. I think Lomo only has a trinocular head and the few that have come
out on Ebay lozacilazo beloved, I guess because of its rarity.
's why I'm looking for some Po trinocular head approx. 42mm, Olympus or
other mark
Regards
Francisco
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Re: First test PZO microscope, DIC
Pluta
Reply # 9 on: August 1, 2013, 1:05:39 am

Hugo
Hero
Posts: 815
Reputation 17
Sex:
Zeiss has used these Trinocular with looking on Ebay "Zeiss trinocular" out
quite a bit. Hugo
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Re: First test PZO microscope, DIC
Pluta
Reply # 10 on: August 1, 2013, 9:52:53
am
francisco
Hero
Posts: 752
Reputation 5
Thanks Hugo for the information
Regards
Francisco
In line
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Re: First test PZO microscope, DIC
Pluta
Reply # 11 on: August 1, 2013, 9:57:33
am
ABEL
Technical Staff
Manager
Hero
Posts: 1774
Reputation 11

Congratulations on the DIC. The PZO not know you could have a DIC. It's an
awesome microscope.
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Re: First test PZO microscope, DIC
Pluta
Reply # 12 on: August 1, 2013, 10:54:31
am
Jose Ricardo
User Jr
Posts: 67
Reputation 0
. Hola Francisco ; Regarding what you state that "any combination of
condenser and objective prism gives me the same effect" this should not be.
The combination of prism and lens is the featuring more sharpness in the
image and is clearly the best. If it is true that the effect of December with the
other combinations are seen, but the image is significantly worse. You must
make sure that the polarizing lenses are effectively cross. With the analyzer
no problem because it takes your system fixing (if you have it in position
crossed 45 degrees). In the case of the capacitor; This position does not have
a fixed point is mounted to "eye." What I do is align the screw of the capacitor
with the reference mark of the polarizer. And I adjust the polarizer on the "X"
position. So I make sure that they are indeed crossed. acquired on Dec. When
the polarizer had no condenser, replaced it with a polarized lens I had. The
results were somewhat variable from one session to another, depending on
the position of the polarizer that day. Greetings.
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Re: First test PZO microscope, DIC
Pluta
Reply # 13 on: August 1, 2013, 13:01:04
pm
francisco
Hero
Posts: 752
Reputation 5
Thanks Abel.
DIC is an old I think dates from approx. 1960 - 1970
Jose Ricardo Thanks for the info, I'm still not familiar with this system and not
doing something correctly. I appreciate your advice and information
I was fortunate to acquire joint DIC in very good condition, I think it was new
for any sign of use is observed.
arrive with the polarizer fitro condenser and this is marked, like that of "head
"with an X to indicate when they are crossed, in fact no sample in the field of
view and the two filters in the x position the image is completely black.
microscope if you had more than evident signs of having been treated with
little " dedication "
Some photographs System

8/17/2014 First test microscope PZO, DIC Pluta
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DSC00094.JPG (59.56 kB, 640x480 - viewed 220 times.) DSC00095.JPG (63.71 kB,
640x480 - viewed 218 times.) DSC00108.JPG (50.67 kB, 640x480 - viewed 207
times.) DSC00105.JPG (54.12 kB, 640x480 - seen 219 times).
8/17/2014 First test microscope PZO, DIC Pluta
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Re: First test PZO microscope, DIC
Pluta
Reply # 14 on: August 1, 2013, 13:56:58
pm
Jose Ricardo
User Jr
Posts: 67
Reputation 0
If indeed it is the right place if you see the dark field. What I wanted to say is
that the capacitor has no fixed position mounting and must be aligned by eye.
If it is not graduation polarizer is incorrect. Dec. My buy parts. Specifically three
Ebay sellers: head, condenser and condenser polarizer. The entire team
seemed too what they asked for. microscope also was / is in a rather poor
state. Although I have removed almost everything to clean and grease, dust
still has in the next lens diaphragm Illuminator (this cleanup is pending). With
the 10x objective this powder as well as deficiencies in lighting (gradient color
temperature of the lamp filament is seen) is appreciated. The latter hope to
correct going Led lighting and placing a diffuser directly in front (like the BH2
and the like). Salu2.
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