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Laboratory name
Location
Head/Responsible person
Standard Operating Procedure (SOP)
Use and maintenance of
class I and class II biological safety cabinets
Code
!ersion no"
#ate of release
Page 1 of 15
Content
1. Scope
2. Definitions and abbreviations
3. Personnel qualifications
$"% &edical fitness
$"' (ducation and training
4. Procedure
)"% Principle
)"' Samples
)"$ (*uipment and materials
)") Reagents and solutions
)"+ #etailed instructions for use
)", Recording and reporting
)"- .uality control
)"/ Safety precautions
5. elated documents
!nne" 1# $easurements of do%n%ard airflo% velocity in a class && 'SC.
!nne" 2# 'SC lo(boo) * daily+%ee)ly maintenance form
!nne" 3# 'SC servicin( form
Compiled by (0amined by 1ppro2ed by Replaced 3e4 2ersion
3ame Code Code
#ate
Signature
Laboratory area 3o of copies Reason for c5ange
Institution
Laboratory name
Location
Head/Responsible person
Standard Operating Procedure (SOP)
Use and maintenance of
class I and class II biological safety cabinets
Code
!ersion no"
#ate of release
Page 2 of 15
1. Scope
65is SOP describes optimal operation of t5e biological safety cabinet t5roug5 regular ser2icing7
certification and pre2enti2e maintenance" 65e biological safety cabinet is a fragile7 precision
piece of e*uipment intended for protecting t5e user (t5e 68 laboratory 4or9er) from airborne
aerosols t5at may cause infection" 8ot5 class I and class II biological safety cabinets are
ade*uate for 68 laboratories"
2. Definitions and abbreviations
anemometer
Instrument for measuring t5e 2elocity of air"
downflow
!ertical airflo4 inside t5e biosafety cabinet"
exhaust airflow
1ir lea2ing t5e biosafety cabinet (recirculated to t5e room or ducted to t5e outdoors)"
inward airflow
1ir entering t5e cabinet: it is e*ui2alent to e05aust airflo4"
8SC biological safety cabinet
8SL biosafety le2el
H(P1 5ig5;efficiency particulate air
PP( personal protecti2e e*uipment
UPS uninterrupted po4er supply
U! ultra2iolet lig5t: for t5is application it s5ould be U!;C (s5ort 4a2e7 germicidal)
<&6 good microbiological tec5ni*ues = 4or9ing met5ods applied to eliminate or minimi>e
e0posure to pat5ogens7 e"g" aerosols7 splas5es7 accidental inoculation
3. Personnel qualifications
3.1 Medical fitness
In accordance 4it5 national la4s and practices7 arrangements s5ould be made for appropriate
5ealt5 sur2eillance of 68 laboratory 4or9ers
before enrolment in t5e 68 laboratory:
at regular inter2als t5ereafter7 annually or bi;annually:
after any bio5a>ard incident:
at t5e onset of 68 symptoms"
Ideally7 indi2idual medical records s5all be 9ept for up to %? years follo4ing t5e end of
occupational e0posure"
Laboratory 4or9ers s5ould be educated about t5e symptoms of 68 and pro2ided 4it5 ready
access to free medical care if symptoms arise"
Institution
Laboratory name
Location
Head/Responsible person
Standard Operating Procedure (SOP)
Use and maintenance of
class I and class II biological safety cabinets
Code
!ersion no"
#ate of release
Page 3 of 15
Confidential HI! counselling and testing s5ould be offered to laboratory 4or9ers" Options for
reassignment of HI!;positi2e or immuno;suppressed indi2iduals a4ay from t5e 5ig5;ris9 areas
of t5e 68 laboratory s5ould be considered"
1ll cases of disease or deat5 identified in accordance 4it5 national la4s and/or practice as
resulting from occupational e0posure to biological agents s5all be notified to t5e competent
aut5ority"
3.2 Education and training
8asic education and training must be gi2en on t5e follo4ing topics
potential ris9s to 5ealt5 (symptoms of 68 disease and transmission):
precautions to be ta9en to minimi>e aerosol formation and pre2ent e0posure:
5ygiene re*uirements:
4earing and use of protecti2e e*uipment and clot5ing:
5andling of potentially infectious materials:
laboratory design7 including airflo4 conditions:
pre2ention of incidents and steps to be ta9en by 4or9ers in t5e case of incidents
(bio5a>ard incidents7 c5emical7 electrical and fire 5a>ards):
good laboratory practice and good microbiological tec5ni*ues:
organi>ation of 4or9 flo4 from clean to dirty areas:
4aste management:
use of anemometer (operation7 identification of malfunctions7 maintenance)"
65e training s5all be
gi2en before a staff member ta9es up 5is/5er post:
strictly super2ised:
adapted to ta9e account of ne4 or c5anged conditions: and
repeated periodically7 preferably e2ery year"
4. Procedure
4.1 Principle
1 class I 8SC is an open;fronted 2entilated cabinet 4it5 a unidirectional in4ard airflo4 a4ay
from t5e operator" (05aust air is 5ard;ducted t5roug5 H(P1 filters (@igure %)" 65e H(P1 filter
traps AA"A-B of particles of ?"$ Cm in diameter and
AA"AAB of particles of greater or smaller si>e" 65e class I 8SC pro2ides protection for t5e
4or9er and t5e en2ironment but does not protect t5e product (specimens7 strain cultures)
against contamination"
1 class II 8SC additionally pro2ides protection of t5e product (specimens7 strain cultures)
against contamination by recirculating part of t5e H(P1;filtered air in a laminar 2ertical flo4
inside t5e cabinet so t5at a Dcurtain D of clean air descends across t5e 45ole 4or9ing surface" 1
class II 8SC s5ould be 2ented to t5e outside t5roug5 a t5imble or 4it5 5ard ducting (@igure ')"
Institution
Laboratory name
Location
Head/Responsible person
Standard Operating Procedure (SOP)
Use and maintenance of
class I and class II biological safety cabinets
Code
!ersion no"
#ate of release
Page 4 of 15
8ot5 class I and II 8SCs must be installed under t5e super2ision of an engineer from (or
aut5ori>ed by) t5e manufacturing company"
4.2 Samples
Specimens for isolation and culture of tubercle bacilli"
Cultures of tubercle bacilli and ot5er mycobacterial species"
4.3 Equipment and materials
4.3.1 PPE
Personnel s5ould be e*uipped 4it5 appropriate personal protecti2e e*uipment (see SOP on PP()"
4.3.2 BSCs
Class I and II 8SCs s5ould 5a2e a 2isible front display of current face 2elocity"
8SCs s5ould be 2ented to t5e outside in accordance 4it5 6able %"
,i(ure 1. Sc-ematic dia(ram of a Class & biolo(ical safety cabinet.
17 front opening: 87 sas5: C7 e05aust H(P1 filter: #7 e05aust plenum
(Source Laboratory biosafety manual7 $rd ed" <ene2a7 Eorld Healt5 Organi>ation7 '??))
Institution
Laboratory name
Location
Head/Responsible person
Standard Operating Procedure (SOP)
Use and maintenance of
class I and class II biological safety cabinets
Code
!ersion no"
#ate of release
Page 5 of 15
,i(ure 2 Sc-ematic dia(ram of a class &&'1 biolo(ical safety cabinet
17 front opening: 87 sas5: C7 e05aust H(P1 filter: #7 supply H(P1 filter: (7 negati2e;pressure
e05aust plenum: @7 blo4er: <7 H(P1 filter for supply air" Connection of t5e cabinet e05aust to
t5e building e05aust air system is re*uired"
(Source Laboratory biosafety manual7 $rd ed" <ene2a7 Eorld Healt5 Organi>ation7 '??))
.able 1. Differences bet%een Class & and class && biolo(ical safety cabinets
(Source Laboratory biosafety manual7 $rd ed" <ene2a7 Eorld Healt5 Organi>ation7 '??))
8SC class @ace 2elocity
(m/s)
1irflo4 (B) (05aust system
Recirculated (05austed
I
a
?"$,
b
? %?? Hard duct
II1% ?"$/=?"+% -? $?
65imble
connection
c
II1'
a
?"+% -? $?
65imble
connection
c
II8%
a
?"+% $? -? Hard duct
II8'
a
?"+% ? %?? Hard duct
Institution
Laboratory name
Location
Head/Responsible person
Standard Operating Procedure (SOP)
Use and maintenance of
class I and class II biological safety cabinets
Code
!ersion no"
#ate of release
Page / of 15
a
1ll biologically contaminated ducts are under negati2e pressure or are surrounded by negati2e pressure
ducts and plenums"
b
3ote t5at according to t5e standard (3 %'),A7 face 2elocity s5ould be 4it5in t5e
range ?"- ; % m/s
c
@or safety reasons in dealing 4it5 ris9 group $ pat5ogens (68 bacilli)7 e05aust to t5e
room s5ould be a2oided"
65e 8SC s5ould be located a4ay from sources of air disturbance suc5 as doors7 4indo4s7 air;
conditioning7 2entilation supply or return outlets7 e05aust fans7 etc" Personnel traffic in front of t5e
8SC s5ould be minimi>ed to a2oid disrupting t5e airflo4"
65e 8SC must be connected to a suitable UPS system (4it5 capacity for at least %+ minutes of
e0tra running time) along 4it5 an in2erter in any location 45ere t5e electricity supply may be
interrupted"
Warning: In ase of !ower failure" infetious !artiles are no lon#er tra!!ed to the $EP% filter
and flow ba& to the o!en front of the BSC" onstitutin# a ma'or bioha(ard for !ersonnel.
4.3.3 %nemometer
1ppropriate t5ermal or rotational anemometer7 portable7 4it5 a telescopic e0tension for t5e
probe7 automatic temperature compensation up to /? FC7 and multiple range selectors (2elocity
range)" 65e 2elocity resolution of t5e measurement s5ould be at least ?"?% m/s in t5e lo4est
2elocity range" 65e 5ousing of t5e instrument s5ould be robust7 sealed against dust and
moisture and able to be disinfected 4it5 -?B alco5ol" 65e 9eypad s5ould be co2ered for
protection"
1nemometers must be calibrated in t5e 5ori>ontal and 2ertical planes according to t5e
manufacturerGs instructions"
4.3.4 Smo&e #enerator
Commercial airflo4 testers are recommended" 65ey are small glass tubes7 sealed at eac5 end"
8ot5 ends are bro9en off 4it5 t5e tool pro2ided and a rubber bulb is fitted to one end" Pressing
t5e bulb to pass air t5roug5 t5e tube causes it to emit 45ite smo9e"
4.4 Reagents and solutions
-?B alco5ol
4.5 etailed instructions for use
4.).1 Certifiation
Certification must be done by a qualified service professional !efore t-e 'SC is put into
service and annually t-ereafter. 65e 8SC must be re;certified 45ene2er it 5as been relocated7
ser2iced or repaired and after H(P1 filters 5a2e been c5anged"
Re;certification s5ould include tests for cabinet integrity7 H(P1 filter lea9s7 do4nflo4 2elocity
profile7 face 2elocity7 negati2e pressure/2entilation rate7 air;flo4 smo9e pattern7 and alarms and
interloc9s" Optional tests for electrical lea9s7 lig5ting intensity7 ultra2iolet lig5t intensity7 noise
le2el and 2ibration may also be conducted"
Institution
Laboratory name
Location
Head/Responsible person
Standard Operating Procedure (SOP)
Use and maintenance of
class I and class II biological safety cabinets
Code
!ersion no"
#ate of release
Page 0 of 15
Special training7 s9ills and e*uipment are re*uired to perform t5ese tests
and it is 5ig5ly recommended t5at t5ey are underta9en by a *ualified professional"
4.).2 *aily use
S4itc5 on t5e 8SC airflo4 +=%+ minutes before use (c5ec9 t5e manufacturerGs instructions)"
C5ec9 t5e airflo4 conditions on t5e display (models t5at indicate positi2e7 negati2e or
differential pressure7 4it5 an accuracy of 'B7 may be a2ailable)"
Complete t5e c5ec9;off form (see anne0 %)" If t5e reading is belo4 t5e t5res5old7 do not use
t5e 8SC and immediately alert t5e 5ead of t5e laboratory"
If t5e airflo4 conditions are ade*uate7 4ipe t5e 4or9 surface7 interior 4alls and interior of t5e
glass 4indo4 4it5 -?B alco5ol"
1ssemble all items needed for 4or9 in t5e 8SC to a2oid fre*uent displacements"
Place items so t5at acti2e 4or9 flo4 from clean to contaminated areas across t5e 4or9
surface"
Organi>e t5e 4or9 to minimi>e arm mo2ements" 1rm mo2ements in and out of t5e 8SC
s5ould be 5ori>ontal so as to minimi>e turbulence and pre2ent air inside t5e 8SC from
flo4ing out" Conduct all manipulations 4it5in t5e 8SC as far as possible to4ards t5e bac9 of
t5e unit"
#o not o2ercro4d t5e 4or9ing area as t5is disturbs t5e airflo4" In class II 8SCs7 ne2er allo4
t5e grille to be co2ered by anyt5ing"
Ha2e a small autocla2able 4aste bag and/or a container 4it5 disinfectant for li*uid 4aste
(and for s5arps if needed) inside t5e 8SC" #o not accumulate 4aste in t5e 8SC = remo2e it
45en acti2ities are finis5ed"
1fter use7 allo4 t5e 8SC to operate 4it5in + minutes to allo4 t5e cabinet to purge (to allo4
time for contaminated air to be remo2ed from t5e interior of t5e 8SC)"
Eipe t5e 4or9 surface7 interior 4alls and interior of t5e glass 4indo4 4it5 -?B alco5ol"
If t5e 8SC is e*uipped 4it5 U! lamps7 s4itc5 on t5e U! lig5t and lea2e for a minimum of $?
minutes"
S4itc5 off t5e 8SC fan"
4.).2 +ee&ly maintenane
If t5e 8SC is e*uipped 4it5 U! lamps7 clean t5e U! lamp 4it5 a gau>e pad moistened 4it5 -?B
alco5ol" 1l4ays 4ear glo2es7 ne2er touc5 t5e lamp 4it5 bare 5ands"
4.).2 ,onthly maintenane
-ualitati.e he&
Perform a *ualitati2e c5ec9 4it5 a smo9e generator across t5e entire 4idt5 of t5e 8SC opening"
65e smo9e test is an indicator of airflo4 direction7 not of 2elocity"
1irflo4 smo9e patterns tests are performed to determine 45et5er
Institution
Laboratory name
Location
Head/Responsible person
Standard Operating Procedure (SOP)
Use and maintenance of
class I and class II biological safety cabinets
Code
!ersion no"
#ate of release
Page 1 of 15
t5e airflo4 along t5e entire perimeter of t5e 4or9 access opening is in4ard7
t5ere is reflu0ing to t5e outside at t5e 4indo4 4iper gas9et and side seals"
In addition in class II 8SCs7 t5e smo9e test allo4s to determine 45et5er
airflo4 4it5in t5e 4or9 area is do4n4ard 4it5 no dead spots or reflu0ing7
ambient air passes onto or o2er t5e 4or9 surface"
If t5e 8SC is found to be defecti2e7 alert t5e 5ead of t5e laboratory"
%ir flow measurements
1ir flo4 measurements s5ould be measured using an anemometer" UserGs tests are restricted to
do4n4ard airflo4 measurement in a Class II 8SC"
6o determine airflo4 2elocity o2er a large cross;section7 45ic5 is t5e case for a 8SC7
measurements 5a2e to be ta9en at se2eral points distributed o2er t5e 45ole plane" 65e
a2erage of t5e measured 2alues is assumed to represent t5e a2erage airflo4 2elocity"
65e instrument s5ould be e*uilibrated to ambient temperature before t5e 2oltage difference
is >eroed7 because t5e temperature difference bet4een t5e t4o t5ermistores (one protected
and one cooled by airflo4) is measured and con2erted into air 2elocity"
65e sensor at t5e telescope s5ould be placed at t5e desired point7 directed (most
instruments 5a2e an arro4) according to t5e airflo4: airflo4 o2er t5e sensor s5ould be free
and un5indered"
65e sensor tip must be 9ept stationary in a stable position 45ile a measurement is ta9en"
&easurement at any position s5ould be made o2er H ,? seconds"
Positions and interpretation of measurements are indicated in 1nne0 %"
4." Recording and reporting
(ac5 use of t5e 8SC s5ould be recorded in t5e logboo9 (1nne0 ')" 65ese records are used for
maintenance/ser2ice sc5edules7 specifically for c5ec9ing airflo4 2elocities7 replacing t5e U!
lamp" 8SC ser2icing forms (1nne0 $) s5ould be used for reporting incidents7 accidents and/or
mec5anical problems" 65ey s5ould be 9ept on file for a minimum of % year7 after 45ic5 t5ey may
be arc5i2ed"
1fter re;certification7 t5e ser2ice professional must issue a certificate indicating t5e performance
c5aracteristics of t5e 8SC and safety compliance" 65e most recent inspection certificate must
be displayed close to t5e 8SC" Certificates s5ould be retained in t5e arc5i2es for as long as t5e
8SC is in use in t5e laboratory"
4.# $ualit% control
In case of any problem or malfunction7 an DOut of Ser2iceI notice must be displayed on t5e 8SC
indicating t5at it is not to be used until t5e problem 5as been diagnosed and corrected"
65e 8SC must be re;certified by a *ualified ser2ice professional at least once a year and after
e2ery replacement of filters"
Institution
Laboratory name
Location
Head/Responsible person
Standard Operating Procedure (SOP)
Use and maintenance of
class I and class II biological safety cabinets
Code
!ersion no"
#ate of release
Page 2 of 15
4.& Safet% precautions
4./.1 +aste mana#ement
1t t5e end of eac5 day7 collect autocla2able bags containing contaminated material and
autocla2e as soon as possible"
4./.2 Personal !rotetion
Refer to specific SOP for details of protecti2e clot5ing for use in 68 culture/#S6 laboratories"
4./.3 0ther
3e2er 4or9 in t5e 8SC 4it5 t5e U! lamp on"
Replace U! lamps at t5e end of t5e period of use recommended by t5e manufacturer
(cumulati2e duration of 8SC use is recorded in t5e logboo9)" Use glo2es to remo2e lamps" Eipe
4it5 a disinfectant and discard follo4ing national guidelines for special 4aste"
@umigation of t5e 8SC is re*uired before
replacement of H(P1 filters:
release of t5e 8SC for use after a maJor bio5a>ardous spill:
repair 4or9 re*uiring access to t5e sealed plenum:
ser2ice or replacement of t5e circulation fan or components:
maintenance 4or9 in contaminated areas:
performance tests re*uiring entry into contaminated areas:
mo2ement of t5e 8SC cabinet to anot5er laboratory:
c5anging 4or9 programmes7 e"g" to non;68 4or9:
release of t5e 8SC cabinet for resale or sal2age"
@umigation must be carried out by a *ualified ser2ice professional"
Users are not to ma9e any repairs" Repair and ser2ice of t5e 8SC must be carried out by a
*ualified ser2ice professional"
1ny problem or malfunction detected must be reported to t5e laboratory manager7 45o 4ill
contact t5e maintenance contractor to correct t5e problem/malfunction as soon as possible"
H(P1 filters must ne.er be touhed by personnel and no attempt s5ould be made to clean or
disinfect filters using c5emical or mec5anical met5ods"
5. elated documents
&anufacturerKs manual7 specific to eac5 8SC
Biosafety in mirobiolo#ial and biomedial laboratories7 +t5 ed" Eas5ington7 #C7 '??-" United
States #epartment of Healt5 and Human Ser2ices/Centers for #isease Control and
Pre2ention/3ational Institutes of Healt57 '??- (a2ailable at
5ttp//444"cdc"go2/od/o5s/biosfty/bmbl+/8&8LL+t5L(dition"pdf)"
Primary ontainment for bioha(ards1 seletion" installation and use of biolo#ial safety abinets"
$rd ed" Eas5ington7 #C7 United States" #epartment of Healt5 and Human Ser2ices/Centers for
Institution
Laboratory name
Location
Head/Responsible person
Standard Operating Procedure (SOP)
Use and maintenance of
class I and class II biological safety cabinets
Code
!ersion no"
#ate of release
Page 13 of 15
#isease Control and Pre2ention/3ational Institutes of Healt57 '??-" 12ailable at
5ttp//444"cdc"go2/od/o5s/biosfty/primaryLcontainmentLforLbio5a>ards"pdf
Collins C7 <range M7 Nates &" 0r#ani(ation and !ratie in tuberulosis bateriolo#y" London7
8utter4ort5s7 %A/+"
@urr 1" C2C handboo& of laboratory safety7 +t5 ed" 8oca Raton7 @L7 CRC Press: '???"
Healt5 Canada" Laboratory biosafety manual7 'nd ed" Otta4a7 &inister of Supply and Ser2ices
Canada7 %AA,"
Healt5 Ser2ices 1d2isory Committee" Safe wor&in# and the !re.ention of infetion in linial
laboratories" London7 HS( 8oo9s7 %AA%"
Oent P67 Oubica <P" Publi health myobateriolo#y1 a #uide for the le.el III laboratory" 1tlanta7
<17 United States #epartment of Healt5 and Human Ser2ices7 Centers for #isease Control7
%A/+"
Laboratory biosafety manual7 $rd ed" <ene2a7 Eorld Healt5 Organi>ation7 '??) (a2ailable at
5ttp//444"45o"int/csr/resources/publications/biosafety/EHOLC#SLCSRLLNOL'??)L%%/en/
Laboratory ser.ies in tuberulosis ontrol. Part III1 Culture" <ene2a7 Eorld Healt5 Organi>ation7
%AA/ (EHO/68/A/"'+/)"
,aintenane manual for laboratory e3ui!ment7 '
nd
ed" <ene2a7 Eorld Healt5 Organi>ation7 '??/
(a2ailable at 444"45o"int/entity/diagnosticsLlaboratory/documents/guidance/guidance'/en/)
Rieder HL et al" 4he !ubli health ser.ie national tuberulosis referene laboratory and the
national laboratory networ&" Paris7 International Union 1gainst 6uberculosis and Lung #isease7
%AA/"
Standards 1ustralia/Standards 3e4 Pealand" Safety in laboratories 5 mirobiolo#ial as!ets
and ontainment failities" Sydney7 Standards 1ustralia International7 '??'"
Institution
Laboratory name
Location
Head/Responsible person
Standard Operating Procedure (SOP)
Use and maintenance of
class I and class II biological safety cabinets
Code
!ersion no"
#ate of release
Page 11 of 15
!nne" 1# $easurements of do%n%ard airflo% velocity in a class && 'SC.
!.1 'SC dimensions# 123 cm %idt-4 /3 cm dept-
65e anemometer is placed o2er t5e 4or9 area7 + cm abo2e t5e lo4er edge of t5e front screen"
1
!.2 'SC dimensions# 153 cm %idt-4 /3 cm dept-
65e anemometer is placed o2er t5e 4or9 area7 + cm abo2e t5e lo4er edge of t5e front screen"
0 19 56 94 131 150 cm
1
0 15 45 75 105 120 cm
15
45
1
5
2
6
3 4
7
8
15
45
1
5
2
6
3 4
7
8
Institution
Laboratory name
Location
Head/Responsible person
Standard Operating Procedure (SOP)
Use and maintenance of
class I and class II biological safety cabinets
Code
!ersion no"
#ate of release
Page 12 of 15
!.3 'SC dimensions# 113 cm %idt-4 /3 cm dept-
65e anemometer is placed o2er t5e 4or9 area7 + cm abo2e t5e lo4er edge of t5e front screen"
0 22 67 112 157 180 cm
1
!.4 &nterpretation of measurements
&easurements are made for eac5 position indicated in t5e 1nne0es abo2e"
65e time for measurement is ,? seconds per measuring point"
Report measurements in t5e table
Position 1ir flo4 2elocity (m/s)
%
'
$
)
+
,
-
/
6otal
12erage (6otal / /)
&nterpretation
1ccording to (3 %'),A'???
1ir flo4 2elocity s5ould be 4it5in t5e range ?"'+ ; ?"+? m/s" 1dditionally no indi2idual
measurement s5ould differ more t5an '?B of t5e 2alue indicated by t5e manufacturer
Compare t5e a2erage (determined) LLLLLLLm/s to t5e reference of t5e manufacturer
LLLLLLLLLm/s Q/; '?B
15
45
1
5
2
6
3 4
7
8
Institution
Laboratory name
Location
Head/Responsible person
Standard Operating Procedure (SOP)
Use and maintenance of
class I and class II biological safety cabinets
Code
!ersion no"
#ate of release
Page 13 of 15
1ccording to 3S@ )A
Re*uires t5e compliance to t5e manufacturersG set points or do4n flo4 2elocity 4it5 a de2iation
of ?"?'+m/s from nominal set point"
Compare t5e a2erage (determined) LLLLLLLm/s to t5e reference of t5e manufacturer
LLLLLLLLLm/s Q/; ?"?'+m/s
Institution
Laboratory name
Location
Head/Responsible person
Standard Operating Procedure (SOP)
Use and maintenance of
biosafety cabinet class I and class II
Code
!ersion 3o"
#ate of releas'
Page 14 of 15
!nne" 2# 'SC lo(boo) * daily+%ee)ly maintenance form
#ate
6ime of
use
Cumulati2e
duration of
use
Cumulati2e duration
of use of U! lamps OperatorGs name
!isual
alarm
Sound
alarm
Smo9e
test
1irflo4
m/s
(for class II 8SC)
Obser2ations
C5ange U! lamps
after RRR 5ours of
use (according to
manufacturerGs
recommendations
)
Institution
Laboratory name
Location
Head/Responsible person
Standard Operating Procedure (SOP)
Use and maintenance of
biosafety cabinet class I and class II
Code
!ersion 3o"
#ate of releasing
Page 15 of 15
!nne" 3# 'SC servicin( form
&.5$ &D56.&,&C!.&76
(*uipment 8SC CL1SS I OR CL1SS II 8rand name
Purc5ase date &odel/type
Location 4it5in laboratory Serial no"
Earranty e0piry date
&anufacturer 6el
1ddress"
Contact person
6ec5nical ser2ice representati2e 6el
P5&7D&C&.8#
#ate Operation Operator
,!&L95 5:56.S
#ate (2ent
Correcti2e
action ta9en
Operator