ANTI-AGING

citrustem
TM
Orange stem cells
Organizes the inner
structure of the
skin for a younger
appearance
www.provitalgroup.com
Exclusive N.A. Distributor
CENTERCHEM, INC. NORWALK, CT
Ph: 203-822-9800 Fax: 203-822-9820
www.centerchem.com


Orange stem cells. Organizes the inner structure of the skin for a younger appearance
citrustem
TM
What are plant stem
cells?
They are undifferentiated cells that have the
capacity to regenerate the whole plant.
Its use is a very sustainable way to obtain the
benefits of plants because you dont need to
cultivate them, no land, no pesticides, less
water is needed, and they are absolutely
traceable and available at convenience.
citrustem
TM
, obtained from orange stem cells with anti-aging effect, is the first
launching of Provital Stem Cells Collection
Skin aging and order
As skin ages, collagen and elastin fibers, that form the
extracellular network, become disordered, unstructured and
sparse.
The dermis loses density and skin shows aging signs:
The microrelief structure becomes more
anisotropic resulting in a loss of softness
and velvety touch.
The skin elasticity decreases and wrinkles appear.
Keeping the skin inner structure
organized is essential for a younger
looking skin
citrustem
TM
Organizes and redensifies
the dermis structure.
Restores the extracellular
network improving cells
adhesion.
Recovers the quality of a
young skin.
...The architect of skin
Orange stem cells
Cell Treatment
% vs. Control
P17-NHDF
100
108
111
123
Control
citrustem
TM
(0.03%)
citrustem
TM
(0.1%)
citrustem
TM
(0.3%)
P17-NHDF
P17-NHDF
P17-NHDF
P17-NHDF
ANTI-AGING
In vitro efcacy
All these genes are related to quality and structure of
extracellular matrix:
Improvement of extracellular matrix structure
Elastin: the most important protein for good skin appearance.
Collagen VI: cell adhesion properties and a
diverse range of extracellular matrix protein
binding activities.
ADAM metallopeptidase: facilitates collagen function and assembly.
Gene % vs. Control
ADAM metallopeptidase with
thrombospondin type 1 motif, 2
Elastin
245
251
Collagen, type VI, alpha 1 239
TGK is an essential enzyme for the epidermis. An
increase in its synthesis means better assembly
and organization of epidermal structures:
Stimulation of TGK production in the epidermis
Aged fibroblasts increase their proliferation
capacity with citrustem
TM
.
The synthesis of collagen and other key
elements of the extracellular matrix grow.
Stimulation of fibroblasts proliferation
citrustem
TM
(0.03%)
CONTROL
T
G
K

I
N
C
R
E
A
S
E

V
S
.

C
O
N
T
R
O
L
600
500
400
300
200
100
0
citrustem
TM
(0.1%)
citrustem
TM
(0.3%)
KERATINOCYTE CULTURES
In vivo efcacy
The stratum corneum is more cohesive, with
fewer scales.
The skin is smoother and recovers its velvety touch.
Desquamation regulation
Product T0 % increase
5.95
17.50*
11.6*
4.20
4.00
Placebo
citrustem
TM
(3%)
citrustem
TM
- placebo
T56
4.45
4.70
The improvement on the microrelief is the
visible result of an organized and better
structured dermis.
Microrelief improvement
Product T0 % increase
0.09
10.15*
10.1*
0.633
0.613
Placebo
citrustem
TM
(3%)
citrustem
TM
- placebo
T56 - T0
0.001
0.062
T56
0.634
0.675
T0
T56
With citrustem
TM
, the skin will recover the
biological elasticity that had 12 years ago.
Biological elasticity Ur/Uf (R7) by Cutometer

(*) statiscally significant

20 women between 41 and 55 years old, who used a 3% formula of citrustem
TM
, compared with a placebo formula. Applied twice a day for 56 days.
Viscous liquid
White - pale yellow color
Aqua (Water), Sodium Hydroxide (10%) E
Glyceryl Caprylate, Glyceryl Undecylenate D
Bentonite, Xantham Gum
Acrylates/C10-30 Alkyl Acrylate Crosspolymer
Simmondsia Chinensis (Jojoba) Seed Oil
C
B Cyclopentasiloxane
Oleyl Erucate
Dimethicone, Polysilicone-11
Oleth-20
Tocopherol
Deion Ster. Water
Glycerin
Aqua (Water), Citric Acid (10%)
Pentylene Glycol
A
INCI / PCPC
RECOMMENDED DOSE
SOLUBILITY
APPEARANCE
2 - 4 %
Soluble in aqueous solutions
citrustem
TM
, obtained from orange
stem cells, organizes, restores
and redensifies the dermis
structure to recover the quality of
a young skin
PROPERTIES
Orange stem cells. Organizes the inner structure of the skin for a younger appearance
citrustem
TM
ANTI-AGING
cosmetic applications
Technical specications Formulation
Global anti-aging lines
Cosmetic products for skin rejuvenation
Anti-aging preventive cosmetics
Cellular regenerator
Intensive facial treatments
Treatment make-up
citrustem
TM
72800 Ageless Restructuring Serum
Parfum (Fragrance) G
citrustem
TM
F
0.68
0.90
1.00
0.30
4.00
8.00
1.00
2.00
3.00
0.20
69.12
3.00
0.15
2.50
% (w/w)
0.15
4.00
V01-2013