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05












BIOHIT


M-LINE


TECHNICAL


SERVICE MANUAL














Page 2 of 42 Ver.03 22.02.05



Table of Contents

1 Product Description...................................................................................................... 3
2 Protective tip cone filters and filter ejection .................................................................. 4
3 Maintenance................................................................................................................. 4
3.1 Daily cleaning of the outer surface of the pipettor ................................................. 4
3.2 Cleaning and decontaminating the lower parts ..................................................... 5
3.2.1 Single channel pipettors: disassembling, regreasing and cleaning................ 5
3.2.2 Single channel pipettors: reassembling ......................................................... 7
3.2.3 Cleaning stop surfaces .................................................................................. 8
3.2.4 Multichannel pipettors: disassembling, regreasing and cleaning ................... 9
3.2.5 Multichannels pipettors: reassembling......................................................... 12
3.3. Sterilization of the pipettor ...................................................................................... 13
4 Testing the performance and recalibration................................................................. 14
4.1 Testing the performance ..................................................................................... 14
4.2 Recalibration....................................................................................................... 14
5 Disassembling and reassembling the handle............................................................. 15
5.1 Disassembling single channel models m10, m20, m100, m200 and m1000:...... 15
5.2 Reassembling single channel models m10, m20, m100, m200 and m1000 ....... 17
5.3 Disassembling model m5000.............................................................................. 18
5.4 Reassembling model m5000............................................................................... 20
5.5 Disassembling MCP-models .............................................................................. 22
5.6 Reassembling MCP models............................................................................... 24
6 Spare parts................................................................................................................. 27
6.1 Handle assembly: m10, m20, m100, m200 and m1000 single channel pipettors27
6.2 Handle assembly m5000..................................................................................... 28
6.3 Model m10 .......................................................................................................... 29
6.4 Model m20 .......................................................................................................... 30
6.5 Model m100 ........................................................................................................ 31
6.6 Model m200 ........................................................................................................ 32
6.7 Model m1000 ...................................................................................................... 33
6.8 Model m5000 ...................................................................................................... 34
6.9 Handle assembly; MCP m10, MCP m100 and MCP m300............................... 35
6.10 Final assembly MCP models m10, m100 and m300........................................... 36
6.11 Dispensing Head, MCP m10............................................................................... 37
6.12 Dispensing Head, MCP m100............................................................................. 38
6.13 Dispensing Head, MCP m300............................................................................. 39
6.14 List of tools and accessories............................................................................... 40
7 Warranty information.................................................................................................. 41
8 Certificate of Conformity............................................................................................. 42


Page 3 of 42 Ver.03 22.02.05


1 Product Description

Your new Biohit mLINE is an autoclavable air displacement pipettor. The attractive and
ergonomical design of the mLINE pipettor together with its very low pipetting forces reduce
the risk of repetitive strain injuries (RSI). The pipettor is designed for both right- and left-
handed use.

The special tip cone design of the pipettor offers the possibility to use replaceable Safe-
Cone filters to help prevent the contamination and damage of the pipettor. The operating
button of the mLINE allows safe and easy filter ejection. The pipettor utilizes disposable
tips.










































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2 Protective tip cone filters and filter ejection

The tip cone design of the Biohit mLINE pipettors (> 10 l) allows the use of Biohit Safe-
Cone filters in the tip cones. These removable filters prevent liquids and aerosols from
entering the pipettor and thus protects the pipettor from contamination and damage.

The Safe-Cone filters are available as Standard or Plus versions. It is recommended to
use the Standard filter for general applications and the Plus filter for more demanding
applications such as cell culture, bacterial and virological work and molecular biology.
Filters need to be changed regularly. The interval for a filter replacement is application
dependant but the recommendation is to change the filter daily (after 50 250 pipetting
cycles) and always in case of over-aspiration.
The Safe-Cone filters can be removed with the unique filter ejector without touching the
filters by hand. Remove the operating button cap and eject the filter by pressing the
operating button down. Make sure that the filter is disposed into a suitable waste
container. Clean the tip cone if needed and put the new filter in place.


3 Maintenance

Biohit mLINE pipettors have been designed for easy in-house service. If the pipettor is
used daily it is recommended to clean/decontaminate and check the performance of the
pipettor every three months. Biohit also provides complete repair and recalibration service
for your pipettor including a service report and performance certificate.

Note: Please make sure that the pipettor has been decontaminated before you send it to
us or your local representative. Please advise if any hazardous material has been used
with your pipettor.

Note: The use of the tip cone filters may prolong the interval of the service. Change the
filters regularly.

Note: It is recommended to always use gloves when cleaning the pipettor.


3.1 Daily cleaning of the outer surface of the pipettor

Your Biohit mLINE pipettor should be checked every day for cleanliness. To clean and
decontaminate the outer surface of your pipettor use Biohit Proline Biocontrol (Cat.no.
724004, 5 litres) and a soft lint-free cloth. It is also possible to use ethanol (70%),
isopropanol (60%) or a mild detergent as a cleaning agent.
Gently clean the surface of the pipettor with moistened cloth and wipe dry. Pay special
attention to the tip cone. Change the tip cone filter.







Page 5 of 42 Ver.03 22.02.05

3.2 Cleaning and decontaminating the lower parts

If your pipettor is in daily use it is recommended to clean/decontaminate and grease the
pipettor every three months. To clean and decontaminate the lower parts of the pipettor
follow these steps:

3.2.1 Single channel pipettors: disassembling, regreasing and cleaning

1. Eject the tip cone filter





2. Unscrew the tip ejector collar counter
clockwise and remove it.






3. Pipettors m10, m20, m100, m200 and m1000:
Unscrew the tip cone holder counter clockwise
and carefully remove it with the tip cone.




Pipettor m5000:
Unscrew the tip cone cylinder counter
clockwise and remove it.




4. Remove the piston (if needed to replace, otherwise it is not
required to remove it during basic maintenance). Turn the
pipettor upside down, push it against a table, hold the
white collar with one hand and unscrew the piston counter
clockwise with another hand.





5. Clean the tip ejector collar, the tip cone holder, the tip cone (cylinder) and the piston
with Biohit Proline Biocontrol, ethanol (70%), isopropanol (60%) or mild detergent
and soft lint-free cloth.



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6. Clean the interior of the tip ejector collar and the tip cone (cylinder) with a cotton
swab. Be careful with the pipettors m10, m20 and m100 so that the seal inside the
tip cone will not damage.
7. Rinse the parts with distilled water if needed and let the parts dry.
8. Pipettors m10, m20, and m100:
Put a thin layer of grease on the piston:



Pipettors m200 and 1000:
Put a thin layer of grease around the seal:






Pipettor m5000:
Put a thin layer of grease on the seal and the interior of the tip cone cylinder:





m10 and m20:
Grease on this
area only
m100: Grease
on this area only
m200: Grease
on the small
seal only
m1000:
Grease on the
seal only
Grease on
the seal
Grease on the
inner surface of
the tip cone
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Note: Avoid excess grease. Use only the grease provided with the pipettor (731140, 2g
tube).

Note: The spare part code for the grease is 731141 (tube 20g). The 731140 (2g tube)
provided along with the pipettor package is not available as a spare part.

Note: Before reassembling check that no lint or particles are on the surface of the piston.

Decontamination:
For complete decontamination of the lower parts place the tip ejector collar, tip cone
holder, tip cone and tip cone cylinder (only the model m5000) into a beaker containing
Biohit Proline Biocontrol and leave for at least 30 minutes. Wipe the piston with Biohit
Proline Biocontrol and the lint-free cloth. Rinse the parts with distilled or sterile water. Let
the parts dry. Grease the piston and seal according to the instructions given earlier in this
chapter.

3.2.2 Single channel pipettors: reassembling

1. Reinstall the piston (if it has been removed, removal of the piston
is not necessarily needed during normal maintenance). Turn the
pipettor upside down, push it against a table, hold the white collar
with one hand and screw the piston clockwise with another hand.






2. Pipettors m10, m20, m100, m200, m1000:
Carefully place the tip cone on the piston and attach it
by screwing the tip cone holder clockwise.



Pipettor m5000:
Carefully place the tip cone cylinder on the piston and
screw clockwise. Make sure that the tip cone (cylinder)
is properly tightened. Avoid over tightening.



3. Attach the tip ejector collar by screwing it clockwise.





4. Place the new tip cone filter on its place with forceps



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5. Press the operating button several times to ensure that the grease has spread
evenly.

6. Check the performance of the pipettor.

Note: It is always necessary to check the performance of the pipettor after in-house
service or maintenance.

3.2.3 Cleaning stop surfaces

If there is a problem with the accuracy of the pipettor or the pipettor is very dirty, the
cleaning of the critical stop surfaces might be required.

1. Remove tip ejector collar, tip cone holder, tip cone and piston as described above
2. Turn the volume to the maximum.
3. Insert a 1000ul tip into the machinery: push upwards and simultaneously turn
clockwise. The gray plastic part is connected to the machinery with a bayonet type
of connection. Piston spring comes out with a metallic sleeve that acts as a stop
surface.











4. Clean the stop surface thoroughly with ethanol and apply some compressed air
inside the machinery.










5. Insert the piston spring and metallic sleeve into the machinery with the help of a tip.
Push upwards and turn counter clockwise.






Stop surface
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6. Reassembly the rest of the parts as described above.

3.2.4 Multichannel pipettors: disassembling, regreasing and cleaning

1. Unscrew the dispensing head by turning it counter clockwise and remove it.










2. Push down the piston support plate as far as it goes (for example
with a pen)

Note: It is very important that the pistons are at the lowest position
during service. Otherwise the reassembly of the pistons and tip
cones is tricky.








3. Push down the tip ejector bar locking piece
(731145) with one hand and simultaneously
hold the tip ejector bar at one end with another
hand. Remove the tip ejector bar by pulling it
out.






4. Attach a tip onto the tip cone. Push the tip cone
upwards (it is spring loaded) and turn it counter
clockwise. Pull the tip cone out of the tip cone
housing






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5. Pull out the piston. It is connected to the piston support plate with a magnet. Please
insert the piston into the tip cone if you store them on the table. That way piston wont get
damaged and collect dirt. Remove all tip cones and pistons.










6. Clean the tip cone housing, the tip ejector bar, the tip cones and the pistons with Biohit
Proline Biocontrol, ethanol (70%), isopropanol (60%) or mild detergent and soft lint-free
cloth.

7. Disassembling tip cones:

Models m10 and m100:

Unscrew the o-ring support nut (m10, 731156) or seal support nut (m100, 726167).












Model m300:

The tip cone can not be disassembled.


The assemblies include the
following parts:











731158 731157 731156 731159 731155
726169 726168 726167 726170 726147
726148
726151
m100
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Clean the parts with Biohit Proline Biocontrol, ethanol (70%), isopropanol (60%) or mild
detergent if needed. Rinse the parts with distilled water if needed and let the parts dry

8. Regreasing.

Models m10 and m100:

Place the parts in an order as indicated in the pictures below. It makes regreasing and
reassembly easier. Apply a thin layer of grease on the seals and pistons only.









Model m300:

Apply a thin layer of grease on the sealing only.









Note: Avoid excess grease. Use only the grease provided with the pipettor (731140, 2g
tube).

Note: The spare part code for the grease is 731141 (tube 20g). The 731140 (2g tube)
provided with the pipettor package is not available as a spare part.

Note: Before reassembling check that no lint or particles are on the surface of the piston.

9. Reassembling

Models m10 and m100:

Tighten the o-ring support nut (m10, 731156) or seal support nut (m100, 726167). Please
make sure the nut is properly tightened but avoid over tightening. Keep the piston inserted
all the time.







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Please move the piston up and down to spread grease evenly.

Model m300:

Insert the piston into the tip cone.










Please move the piston up and down to spread grease evenly.

Decontamination:
For complete decontamination of the lower parts place the tip cone housing, tip cones,
pistons and tip ejector bar into a beaker containing Biohit Proline Biocontrol and leave for
at least 30 minutes. Rinse the parts with distilled or sterile water. Let the parts dry. Grease
the seal and piston as described above.

3.2.5 Multichannels pipettors: reassembling

1. Please make sure that the piston support plate is at its lowest
position.







2. Attach a tip on the tip cone and insert the piston assembly complete (piston+tip cone)
into the tip cone housing. Push it upwards as far as it goes and turn the tip cone clockwise.
Make sure that the tip cone is properly placed.













appr.20m
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3. Reassemble the tip ejector bar. Push down the tip
ejector bar locking piece (731145) with one hand and
simultaneously hold the tip ejector bar with another hand
and push it upwards. Make sure the snap fit will connect
properly.





4.Insert tip cone filters. Attach the dispensing head to the handle and tighten it properly.
Please avoid over tightening. Please make sure that the dispensing head can rotate freely.












3.3. Sterilization of the pipettor

The entire mLINE pipettor can be sterilized by steam autoclaving at 121
o
C, (252
o
F), 1
atm for 20 minutes (please ref. instruction manual for details). Remove the tip cone filter (if
fitted, Chapter 7.3). Put the pipettor into the sterilization bag and place it into the
autoclave. After autoclaving the pipettor must be cooled down at least for two hours before
use. It is recommended to grease the piston/seal after every 10 autoclaving cycle. It is
recommended to check the performance of the pipettor after every autoclaving cycle.
















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4 Testing the performance and recalibration

It is recommended to check the performance of your Biohit mLINE pipettors regularly (e.g.
every 3 months) and always after in-house maintenance. However, the user should
establish a regular testing routine for their pipettors with regard to accuracy requirements
of the application, frequency of use, number of operators using the pipettor, nature of the
liquid dispensed and the acceptable maximum permissible errors established by the user.
(ISO 8655-1.)

4.1 Testing the performance

Performance testing should take place in a draught-free room at 15 30
O
C, constant to
+/- 0.5
O
C and humidity above 50%. The pipettor, tips and the test water should have
stood in the test room a sufficient time (at least 2 hours) to reach equilibrium with the room
conditions. Use distilled or deionised water (grade 3, ISO 3696). Use an analytical balance
with a readability of 0.01 mgs. (ISO 8655-6.)
If the results fall within the specifications, the pipettor is ready for use. Otherwise check
both systematic and random errors and, when necessary, proceed to the recalibration
procedure .

Note: Systematic error (inaccuracy) is the difference between the dispensed volume and
the selected test volume. Random error (imprecision) is the scatter of the dispensed
volumes around the mean of the dispensed volume. (ISO 8655-1)

Note: Biohit specifications are achieved in strictly controlled conditions (ISO 8655-6). The
user should establish own specifications based on the field of use and the accuracy
requirements placed on the pipettor (ISO8655-1).

Note: This method is based on ISO 8655.
4.2 Recalibration

The calibration of your mLINE pipettor has been factory checked and certified at 22
O
C
using grade 3 distilled water according to ISO 3696. The calibration is based on ISO 8655-
6: Gravimetric test method for volumetric instruments. The pipettors specifications are
guaranteed only with genuine Biohit tips. If you find the pipettor to be inaccurate after
performance testing, please follow the instructions below:

1. Remove the lid of the calibration nut, located at the backside of the handle, with the
aid of the calibration tool.
2. Place the hexagonal head of the calibration tool into the hole of the calibration nut.
3. Turn the adjustment lock counter clockwise to decrease and clockwise to increase
the volume.
4. Repeat testing the performance procedure. Continue until the results are correct.

Note: Biohit offers accredited calibration service. Please contact your local Biohit
representative for further information.

Note: Please do not use the plastic mLine calibration tool provided with the pipettor
package for calibration purposes in technical service. The plastic tool has not been
designed for continuous professional use. Please use a standard metallic tool.
Page 15 of 42 Ver.03 22.02.05



5 Disassembling and reassembling the handle

The upper part of the pipettor (handle) must be disassembled if, for example, there is a
problem with the counter or any other inner part. The disassembly of the upper part is not
a normal service routine!
5.1 Disassembling single channel models m10, m20, m100, m200 and m1000:

1) Remove the tip ejector collar, tip cone holder and tip cone

2) Next remove the tip ejector and handle collar by pushing the tip
ejector upwards with your thumb.








3) Turn the pipettor upside down, push it against a table, hold the
white collar with one hand and unscrew the piston counter
clockwise with another hand.






4) Now the following parts have been removed:





















Tip ejector collar
Piston
Tip cone Tip cone holder
Handle collar
Cap
Tip ejector
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5) Remove spring lock, primary and
secondary springs and tip cone
locking ring with the mLine tool
(726201). Push the tool upwards and
simultaneously turn counter
clockwise. The spring lock is locked
with a bayonet type of connection.













6) Pull out the machinery assembly (counter and operating button). Place your fore finger
into the handle. Remove the display by pushing it with the fore finger.










7) Now the following parts have been removed:


8) Clean, repair and replace parts if needed.





Tip cone
locking
ring
Primary
spring
Secondary
spring
Spring
lock
Page 17 of 42 Ver.03 22.02.05


5.2 Reassembling single channel models m10, m20, m100, m200 and m1000

1) Please note that there are two small slots on the machinery body and two small ribs
on the calibration wheel inside the handle (please see the pics below).

















2) Insert the machinery into the handle








3) Make sure that the two ribs on the calibration wheel
go into the slots on the machinery body.









4) The machinery has been reassembled in a right way when the lines of the upper most
counter wheel are visible
Two ribs
Two slots
The lines must
be visible this
way
Page 18 of 42 Ver.03 22.02.05



5) Reassemble the tip cone locking ring, primary spring, secondary spring and spring lock
in a right order. Lock the spring lock with the mLine tool by pushing the spring lock
upwards and simultaneously turning it clockwise. Please place your thumb on the counter
to keep the machinery body still. Otherwise it may slip out of the handle.


Note! Please make sure that the counter lines are clearly visible.

6) Reassemble the window and counter
release nut; the upper snap fit must go first. If
the window is not sitting properly, then the
calibration wheel might not be sitting well. In
that case push the wheel back into its groove.
Replace the display sticker (used for attaching
the display), if needed.


7) Reassemble the tip ejector and handle cover. Screw the piston












9) Reassemble the tip ejector collar, tip cone holder and tip cone.

10) Make sure the operating button and counter works properly

5.3 Disassembling model m5000

1) Remove the tip ejector collar, tip cone holder and tip cone

2) Next remove the tip ejector and handle collar by pushing the tip
ejector upwards with your thumb.


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3) Turn the pipettor upside down, push it against a table, hold the white
collar with one hand and unscrew the piston counter clockwise with
another hand.


4) Now the following parts have been removed:
















5) Remove the tip cone holder with mLine 5000 tool (726202) by pushing the tool upwards
continuously and simultaneously turning counter clockwise


6) Remove spring lock, primary and
secondary springs and tip cone locking ring
with the mLine tool. Push the tool upwards
and simultaneously turn counter clockwise.
The spring lock is locked with a bayonet type
of connection.













Tip ejector collar
Piston
Tip cone
Tip cone
locking
ring
Primary
spring
Secondary
spring
Spring
lock
Handle collar
Cap
Tip ejector
Tip
cone
holder
Page 20 of 42 Ver.03 22.02.05



7) Pull out the machinery assembly (counter and operating button). Place your fore finger
into the handle. Remove the display by pushing it with the fore finger.










9) Now the following parts have been removed:

10) Clean, repair and replace parts if needed.

5.4 Reassembling model m5000

1) Please note that there are two small slots on the machinery body and two small ribs
on the calibration wheel inside the handle (please see the pics below).


















Two ribs
Two slots
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2) Insert the machinery into the handle










3) Make sure that the two ribs on the calibration wheel go
into the slots on the machinery body.







4) The machinery has been reassembled in a right way
when the lines of the upper most counter wheel are visible

5) Reassemble the tip cone locking ring, primary spring, secondary spring and spring lock
in a right order. Lock the spring lock with the mLIne tool by pushing the spring lock
upwards and simultaneously turning it clockwise. Please place your thumb on the counter
to keep the machinery body still. Otherwise it may slip out of the handle.


Note! Please make sure that the counter lines are clearly visible.




The lines must
be visible this
way
Page 22 of 42 Ver.03 22.02.05



6) Reassemble the tip cone locking nut by screwing it counter clockwise by hand until it
clicks


7) Reassemble the window and counter release nut; the upper snap fit must go first. If the
window is not sitting properly, then the calibration wheel might not be sitting well. In that
case push the wheel back into its groove. Replace the display sticker (used for attaching
the display), if needed.

8) Reassemble the tip ejector and handle cover. Screw the piston











9) Reassemble the tip ejector collar and tip cone.

10) Make sure the operating button and counter works properly

5.5 Disassembling MCP-models


1) Remove dispensing head

2) Next remove the tip ejector and handle collar by pushing the tip
ejector upwards with your thumb.




Page 23 of 42 Ver.03 22.02.05




3) Turn the pipettor upside down, push it against a table, hold the
white collar with one hand and unscrew the magnet holder (726166)
counter clockwise with another hand.

Note: Do not hold the white part with forceps; it might damage the
part!

4) Unscrew the locking nut (726165) with the mLine MCP tool (726204). Insert the tool and
turn counter clockwise. Remove all parts and pull out the machinery








5) Place your fore finger into the handle. Remove the display by pushing it with the fore
finger.








6) Now the following parts have been removed:














7) Clean, repair and replace parts if needed. Clean the piston spring (726071) and stop
surface (metallic sleeve) thoroughly with ethanol and apply some compressed air inside
the machinery (ref chapter 3.2.3).




Page 24 of 42 Ver.03 22.02.05



5.6 Reassembling MCP models

1. Please note that there are two small slots on the machinery body and two small ribs on
the calibration wheel inside the handle (please see the pics below).

















2. Insert the machinery into the handle








3. Make sure that the two ribs on the calibration wheel go
into the slots on the machinery body.







4. The machinery has been reassembled in a right way when the lines of the upper most
counter wheel are visible


Two ribs
Two slots
The lines must
be visible this
way
Page 25 of 42 Ver.03 22.02.05




5. Please place your thumb on the counter to keep the machinery body still. Otherwise it
may slip out of the handle. Insert the piston spring (726071).








6. Insert the tip ejection adapter (726163) so that the flat end goes first. Then insert tip
ejection collar (726163) and connecting collar (726164)










7. Insert the locking nut (726165) and tighten it with the mLine MCP tool (726204). Push
down the spring loaded locking nut and simultaneously turn the tool clockwise. Please
make sure the locking nut is properly tightened but avoid over tightening. Make sure that
the connecting collar can be rotated freely. If not, then the nut is over tightened.











8. Reassemble the window and counter release nut; the upper snap fit must go first. If the
window is not sitting properly, then the calibration wheel might not be sitting well. In that
case push the wheel back into its groove. Replace the display sticker (used for attaching
the display), if needed.


Page 26 of 42 Ver.03 22.02.05





9. Reassemble the tip ejector and handle cover. Screw the magnet holder.



10. Attach the dispensing head.




































Page 27 of 42 Ver.03 22.02.05


6 Spare parts

6.1 Handle assembly: m10, m20, m100, m200 and m1000 single channel pipettors




Handle, mline; 726012
Calibration wheel; 726066
Machinery, complete;
Model 10; 726061
Model 20: 726085
Model 100; 726062
Model 200, 726063
Model 1000; 726064
Tip cone locking ring; 726069
Primary spring; 726073
Secondary spring; 726074
Spring lock; 726075
Display;
10; 726022
100; 726026
20: 726082
200; 726027
1000; 726028
Counter release nut;
726068
Display sticker, 5x20pcs
726121
mLine opening tool: 726201
Page 28 of 42 Ver.03 22.02.05


6.2 Handle assembly m5000



















































Handle, mline; 726012
Machinery, complete;
5000; 726065
Tip cone locking ring 5000;
Primary spring; 726073
Secondary spring; 726074
Spring lock; 726075
Display;
5000; 726029
Counter release nut;
726068
Calibration wheel: 726066
Display sticker, 5x20pcs
726121
mLine 5000 opening tool: 726202
Page 29 of 42 Ver.03 22.02.05


6.3 Model m10



















































Cap, set of 5 colours; 726001
Handle collar, 726002
ID window; 726004
ID tag; 726003
Tip ejector; 726005
Calibration nut cover;
Piston 10; 731076
Tip cone complete;
10; 731084
Tip cone 10, upper part,
731075
Tip cone 10 , lower part,
731077
O-rings, 10pcs: 731085
Tip cone holder; 726041
Tip ejector collar; 726051
mLine autoclavable grease, 20g tube:
731141
Page 30 of 42 Ver.03 22.02.05



6.4 Model m20


















































Handle collar, 726002
ID window; 726004
ID tag; 726003
Tip ejector; 726005
Calibration nut cover;
Piston 20; 726084
Tip cone 20; 726083
Tip cone holder; 726041
Tip ejector collar; 726052
Cap, set of 5 colours; 726001
mLine autoclavable grease, 20g tube:
731141
Page 31 of 42 Ver.03 22.02.05


6.5 Model m100



















































Cap, set of 5 colours; 726001
Handle collar, 726002
ID window; 726004
ID tag; 726003
Tip ejector; 726005
Calibration nut cover;
Piston 100; 726034
Tip cone;
100; 726042
Tip cone holder; 726041
Tip ejector collar; 726052
mLine autoclavable grease, 20g tube:
731141
Page 32 of 42 Ver.03 22.02.05



6.6 Model m200


















































Cap, set of 5 colours; 726001
Handle collar, 726002
ID window; 726004
ID tag; 726003
Tip ejector; 726005
Calibration nut cover;
Piston 200; 726035
Tip cone;
200; 726039
Tip cone holder; 726041
Tip ejector collar; 726052
mLine autoclavable grease, 20g tube:
731141
Page 33 of 42 Ver.03 22.02.05



6.7 Model m1000


















































Cap, set of 5 colours; 726001
Handle collar, 726002
ID window; 726004
ID tag; 726003
Tip ejector; 726005
Calibration nut cover;
726006
Piston 1000; 726036
Tip cone;
Tip cone holder 1000;
Tip ejector collar; 726053
mLine autoclavable grease, 20g tube:
731141
Page 34 of 42 Ver.03 22.02.05


6.8 Model m5000



















































Cap, set of 5 colours; 726001
Handle collar, 726002
ID window; 726004
ID tag; 726003
Tip ejector; 726005
Calibration nut cover;
Piston 5000; 726037
Tip cone;
Tip cone holder 5000; 726076
Tip ejector collar
5000; 726054
mLine autoclavable grease, 20g tube:
731141
Page 35 of 42 Ver.03 22.02.05
Connecting collar
and friction o-ring
Locking nut
726165
Magnet holder
726166


6.9 Handle assembly; MCP m10, MCP m100 and MCP m300



















































Machinery complete mline MCP:
0,5-10: 726160
5-100: 726161
30-300: 726162
Calibration Wheel: 726066
Handle: 726012
Counter Release nut:
726068
Display:
0,5-10: 726022
5-100: 726141
30-300: 726142
Display sticker, 5x20pcs
726121
Tip ejection adapter
Piston spring
726071
mLine opening tool MCP: 726205
Page 36 of 42 Ver.03 22.02.05


6.10 Final assembly MCP models m10, m100 and m300



















































Cap, set of 5 colours; 726001
Handle collar, 726002
ID window; 726004
ID tag; 726003
Calibration nut cover;
Tip ejector; 726005
Dispensing head, please see
details in the following pages
Page 37 of 42 Ver.03 22.02.05
Locking bar springs;
2pcs, 731144
Tip cone assembly
complete 10ul;
731160


6.11 Dispensing Head, MCP m10



















































Tip ejector bar locking piece; 731145
Tip cone housing 8ch/10; 726131
12ch/10;726134
Tip ejector bar 8ch/10; 726137
12ch/10;726139
Piston 10; 731155
O-ring support nut ; 731156
O-rings, 2pcs; 731157
Spring support; 731158
Spring; 731159
Piston assembly
complete 10ul;
731187
(includes both
piston and tip
cone complete)
mLine autoclavable grease, 20g tube:
731141
Page 38 of 42 Ver.03 22.02.05
Locking bar springs;
2pcs, 731144
Piston assembly
complete 100ul;
726153
(includes piston
and tip cone
complete)
Tip cone
assembly
complete
100ul; 726150


6.12 Dispensing Head, MCP m100



















































Tip ejector bar locking piece; 731145
Tip cone housing 8ch/100; 726132
12ch/100;726135
Tip ejector bar 8ch/100; 726138
12ch/100;726140
Piston 100; 726147
Seal support nut ; 726167
Seal support spring, 8 pcs; 726168
Seal support, 8pcs; 726169
Seal 100/120, 8pcs 726170
mLine autoclavable grease, 20g tube:
731141
Page 39 of 42 Ver.03 22.02.05
Locking bar springs;
2pcs, 731144
Piston assembly
complete 300ul;
726154
(includes piston and
tip cone complete)
Tip cone assembly
complete 300ul;
726151
(includes also the
spring)


6.13 Dispensing Head, MCP m300



















































Tip ejector bar locking piece; 731145
Tip cone housing 8ch/300; 726133
12ch/300;726136
Tip ejector bar 8ch/300; 726138
12ch/300;726140
Piston 300; 726148
mLine autoclavable grease, 20g tube:
731141
Page 40 of 42 Ver.03 22.02.05


6.14 List of tools and accessories

721009 Biohit forceps for filter replacement
725160 Biohit Holder for mLINE pipettor
726081 Blue cap set, 5 pieces
726200 Service kit, mLine single channel pipettors
726201 mline tool
726202 mLine 5000 tool
726203 mLine calibration tool
726204 mLine MCP tool
726206 Service kit, mLine multichannel pipettors
731141 Grease, 20g tube



Note! Please check always the spare part codes from the official price
lists when ordering





























Page 41 of 42 Ver.03 22.02.05



7 Warranty information

The Biohit mLINE pipettors are covered by warranty for 3 years against defects in
materials and workmanship. Should your mLINE pipettor fail to function at any time, please
contact your local Biohit representative.

ANY WARRANTY WILL, HOWEVER, BE DEEMED AS VOID IF FAULT IS FOUND TO
HAVE BEEN CAUSED BY MALTREATMENT, MISUSE, UNAUTHORIZED
MAINTENANCE OF SERVICE OR NEGLIGENCE OF REGULAR MAINTENANCE AND
SERVICE, ACCIDENTAL DAMAGE, INCORRECT STORAGE OR USE OF THE
PRODUCTS FOR OPERATIONS OUTSIDE THEIR SPECIFIED LIMITATIONS, OUTSIDE
THEIR SPECIFICATIONS, CONTRARY TO THE INSTRUCTIONS GIVEN IN THIS
MANUAL OR WITH OTHER THAN THE MANUFACTURERS ORIGINAL TIPS.

Each Biohit mLINE pipettor is tested before shipping by the manufacturer. The Biohit
Quality Assurance Procedure guarantees that the Biohit mLINE pipettor you have
purchased is ready for use.

Each Biohit mLINE pipettor is CE marked.

































Page 42 of 42 Ver.03 22.02.05



8 Certificate of Conformity