1

S HNH THANH MOT

AP NG MIEN DCH
GS Pham Hoang Phiet
BS Huynh Thanh Bnh
2
.
.
ong hoc mot ap ng mien dch
3
So sanh ap ng th 1 va th 2
MD
Tnh chat
Th 1 Th 2
Dien
tien
Tiem an 1-2 tuan 3-5 ngay
Tang SLKT Tang nhanh Tang rat nhanh
Bnh on 2-3 tuan Nhieu thang- nam
Giam sut Nhanh T t
So
lng
Cao hn th 1 nhieu
Chat
lng
Lp KT IgM IgG
Ai lc vi
KN
+ +++
4
Te bao trnh dien
khang nguyen
HLA lp II TCD4
(peptid 15-34 aa)
HLA lp I TCD8
(peptid 9 aa)
5
Class I MHC Pathway
Viral protein is made
on cytoplasmic
ribosomes
Plasma membrane
Proteasome
degrades
protein to
peptides
Peptide transporter
protein moves
peptide into ER
MHC class I alpha
and beta proteins
are made on the rER
Peptide associates
with MHC-I complex
Peptide with MHC
goes to Golgi body
Peptide passes
with MHC from Golgi
body to surface
Peptide is presented
by MHC-I to CD8
cytotoxic T cell
Golgi body
rER
Globular viral
protein - intact
6
Class II MHC Pathway
Globular
protein
Endocytosis
Protein is processed to
peptides in endosome
or lysosome
Endosome
Lysosome
Fusion of endosome
and exocytic vesicle
Endoplasmic reticulum
Class II MHC
Synthesis
3 chains: , and Ii
Golgi
body
Exocytic vesicle fuses
with endosome
releasing Ii from dimer

Ii
Immunodominant
peptide binds
to class II MHC
Endosome fuses with
plasma membrane
Peptide MHC-II
complex is presented
to CD4 helper T cell
CD4 helper T cell
7
IL2 (IL4)
TB,Tc, (B)
Hoat hoa Lympho T
1
2
3
4
8
Points Concerning Antigen
Processing and Presentation
1. Location of pathogen
viruses in cytosol, MHC class I
pathway, Tc response
extracellular bacteria, MHC class II
pathway, Th2 response, Ab formation
intracellular bacteria, MHC class II
pathway, Th1 response
9
Points Concerning Antigen
Processing and Presentation
2. Peptides derived from both self and
non-self proteins can associate with
MHC class I and class II molecules.
3. Chemical nature of MHC groove
determines which peptides it will
bind.
10
Hoat hoa Lympho bao
T B
Tn hieu
KN
(1)Ti (TCR) KN
(2) CD4 (T
H
)
HLA
II
(APC)
sIg (BCR) KN
Tn hieu
th 2
(1) IFN- (T
H
)
(2) IL1 (APC)
IL2,IL4, IL5
11
Hoat hoa
Lympho B
12
S hp tac gia
ai thc bao,
lympho T va
lympho B
13
Cellular Events in 1
o
Response to
T-dependent Ags
Lag
Clonal selection
Log
IgM
Class switching
Stationary
Decline
Memory Cell Pool
IgM
Memory Cells
IgG
1
o
Ag
14
Cellular Events in 2
o
Response to
T-dependent Ags
Lag phase
Virgin cells
Memory cells
Log phase
Pool size
IgG, IgA or IgE
Stationary
Decline
Sustained
production
IgM
Memory
Cells
IgG
IgG
Memory
Cells
Memory
Pool
Virgin B cell
15
Hieu ng Hapten-tai
Hapten khong co tnh gay MD
Hapten phai gan vi 1 protein tai =>
phc hapten tai (hapten-carrier
complex)
Th nhan dien tai, B nhan dien hapten
Tai th 1 va th 2 phai giong nhau.
16
S hp tac han che trong nhom phu hp mo
(hien tng Zinkernagel va Doherty)
Mouse
Strain A or B
Immunize
with antigen
Period of 7 days
T cells isolated
from lymph nodes
T cell primed with
antigen
Mouse
Strain A or B
Peritoneal macrophage
Antigen
Macrophage presents antigen
Macrophages and
T cells are co-cultivated
Anti X-induced
Macrophage T-cell proliferation
Strain A A yes
Strain A B no
Strain B B yes
Strain B A no
Assay DNA
synthesis
after 48 hr
17
Tc Cell Self MHC Restriction
Mouse
Strain A or B
Immunize
with virus
Period of 7 days
Virus-specific
cytotoxic T cells (CTLs)
isolated from spleen
Virus-specific
CTLs
Mouse
Strain A or B
Purify
Fibroblasts
CTLs and fibroblasts
are co-cultivated
Spleen Fibroblast 51Cr release
CTLs (target cells) from fibroblasts
immunized infected (lysis)
with with
Strain A A yes
Strain A B no
Strain B B yes
Strain B A no
Assay 51Cr
release
Isolate spleen
Infect fibroblasts
with virus and
radiolabel with 51Cr
51Cr labeled fibroblast
presents antigen
18
Self MHC Restriction in the Thymus
4 -
8 low
4 low
8 low
4 + 8 +
TCR
4 + 8 +
TCR
macrophage
Productive TCR
rearrangement
Non-productive TCR
rearrangement
Recognise self MHC
Not recognise self MHC
TCR does not
recognise self antigens
Negative selection
TCR recognises
self antigens
APOPTOSIS
4 + 8 +
TCR
4 - 8 -
Sub-capsular region
Cortex
Cortico-medullary region
4 + 8 -
TCR
4 - 8 +
TCR
vessel
Medulla
19
KN khong le thuoc thymus
Cau truc: ai phan t do trung phan cac n
v nho (lipopolysaccharide, dextran)
Cac epitop giong nhau tren ai phan t lien
ket vi nhieu sIg tao thanh am (hien tng
oi mu) => hoat hoa Lympho B thanh tng
bao => SX KT
KT thuoc lp IgM, khong tao ra tr nh MD
20
Hien tng oi mu