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Force microscopy has become an important tool for quantifying mechanical properties of biological materials. Here we present a method for characterizing nonadherent cells with AFM by mechanically immobilizing them in microfabricated wells. Myeloid (HL60) cells were measured to be a factor of 18 times stiffer than lymphoid (Jurkat) cells.
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Force Microscopy of Nonadherent Cells a Comparison of Leukemia
Force microscopy has become an important tool for quantifying mechanical properties of biological materials. Here we present a method for characterizing nonadherent cells with AFM by mechani…