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hampton research 34 journey ali so vi ejo, ca 92656- 3317 usa
telephone: 800- 452- 3899 or 949- 425- 1321 fax: 949- 425- 1611
emai l: i nfo@hrmai l. com websi te: www. hamptonresearch. com
v o l u m e 1 8
C R Y S T A L L I Z A T I O N
S O L U T I O N S F O R C R Y S T A L G R O W T H
c o nt a c t i nf o r ma t i o n
Business Hours: 7:00 a.m. to 5:00 p.m.
Monday - Friday
(Pacific Standard Time)
Phone: (800) 452-3899 or
(949) 425-1321

Fax: (949) 425-1611
Address: Hampton Research
34 Journey
Aliso Viejo, CA 92656 U.S.A.
c us t o me r s up p o rt s e rv i c e s
Customer Service: Phone: (800) 452-3899 or
(949) 425-1321
Fax: (949) 425-1611
Email: info@hrmail.com
Technical Support: Phone: (949) 425-1321
Fax: (949) 425-1611
Email: tech@hrmail.com
Website: www.hamptonresearch.com
Solutions for Crystal Growth
On the cover: Crystals of Big-R protein of Xyllela fastidiosa.
Produced in Brazilian Synchrotron Light Laboratory (LNLS),
Center for Structural Molecular Biology by Rosicler Lzaro Barbosa.
T A B L E O F C O N T E N T S
1
On the cover: Crystals of Big-R protein of Xyllela fastidiosa.
Produced in Brazilian Synchrotron Light Laboratory (LNLS),
Center for Structural Molecular Biology by Rosicler Lzaro Barbosa.
2 - 33 s c r e e n s
34 - 37 c u s t o m s h o p c r y s t a l l i z a t i o n r e a g e n t s
38 - 59 o p t i m i z e c r y s t a l l i z a t i o n g r a d e r e a g e n t s
60 - 69 s t o c k o p t i o n s k i t s
70 - 105 c r y s t a l l i z a t i o n p l a t e s , h a r d w a r e & a c c e s s o r i e s
106 -115 t o o l s , s e e d i n g & r e s i n
116 - 135 c r y o c r y s t a l l o g r a p h y
136 - 145 c a p i l l a r y m o u n t s & s u p p l i e s
146 - 153 g o n i o m e t e r h e a d s & s u p p l i e s
154 - 157 x e n o n d e r i v a t i z a t i o n
158 - 163 l a b e l s a n d p e n s
164 - 173 b o o k s
174 - 179 p r o t e i n c r y s t a l l i z a t i o n s t a n d a r d s
180 - 267 c r y s t a l g r o w t h 1 0 1
268 - 275 i n d e x
276 - 277 g e n e r a l i n f o r m a t i o n
P A G E S
Here is a recipe to try:
Mosaicity is about 0.5
t0 0.6
Reagent:
Crystal Screen Cryo
Reagent 23
Mix equal amounts of
Glucose Isomerase
and reagent. Vapor
diffusion method.

Mount crystal in
CryoLoop.

Mosaicity may be
a bit more in this
reagent and the
unit cell will shrink a
s c r e e n s
Empire State Building-shaped crystal of neuron guidance receptor protein grown in Hampton Research Crystal Screen reagent 15.
Momchil Kolev, Dorothea Robev and Dimitar Nikolov, Structural Biology Department at Memorial Sloan-Kettering Cancer Center, New York, USA.
T A B L E O F C O N T E N T S
5 p c t

p r e - c r y s t a l l i z a t i o n t e s t
6 - 7 i n d e x

8 - 9 c r y s t a l s c r e e n


10 - 11 p e g r x


12 - 13 p e g / i o n

14 - 15 g r i d s c r e e n s

q u i k s c r e e n

16 - 17 s a l t r x

18 - 19 m e m b f a c

c r y s t a l s c r e e n l i t e

20 - 21 n a t r i x


22 - 23 c r y s t a l s c r e e n c r y o

24 n u c l e i c a c i d m i n i s c r e e n

25 l o w i o n i c s t r e n g t h s c r e e n

26 - 27 s i l v e r b u l l e t s

28 a d d i t i v e s c r e e n

29 d e t e r g e n t s c r e e n

30 - 32 h e a v y a t o m k i t s


P A G E S
Fish shaped protein crystal grown from
Hampton Research crystallization reagents.
Paul Morin and Kevin Kish, Bristol Myers-Squibb,
Princeton, New Jersey, USA.
Crystals of kinase complex optimized from
a Hampton Research Tacsimate based reagent.
Michelle Quiles, GlaxoSmithKline, RTP,
North Carolina, USA.
Crystals of human mineralocorticoid receptor complexed with ligand.
Grown using Hampton Salt Rx screen.

Kevin P Madauss, GlaxoSmithkline, Research Triangle Park,
North Carolina, USA.
PCT Reagent A1/B1 Results PCT Reagent A2/B2 Results Action
Heavy amorphous precipitate Heavy amorphous precipitate Dilute sample 1:1, repeat steps 1-7
Concentrate sample to half the original
volume, repeat steps 1-7
Clear Clear
Perform screen Light granular precipitate Clear
Perform screen Clear Light granular precipitate
Perform screen Light granular precipitate Light granular precipitate
Heavy amorphous precipitate Clear
Perform PCT with B1 & B2 /
perform diagnostic testing
Clear Heavy amorphous precipitate
Perform PCT with B1 & B2 /
perform diagnostic testing
Figure 1. Light Precipitate
Figure 2. Heavy Amorphous Precipitate
n
Determine the appropriate sample concentra-
tion for crystallization screening
n
Conserve sample
n
Optimize sample concentration prior to initial
screens
n
Provide insight to sample homogeneity
a p p l i c a t i o n
f e a t u r e s
The PCT Pre-Crystallization Test is used
to determine the appropriate sample
concentration for crystallization screen-
ing. Sample concentration is a signifi-
cant crystallization variable. Samples too
concentrated can result in amorphous
precipitate, while samples too dilute
can result in clear drops. Precipitate and
clear drops are typical crystallization screen results for reagent conditions which do not promote crystal-
lization and are part of every crystallization screen. However, by optimizing protein concentration for the
screen, the number of clear and precipitate results can often be reduced, which in turn results in more effi-
cient sample utilization while at the same time enhancing the chances for crystallization. PCT can minimize
or prevent situations where a screen results in an over abundance of precipitate or clear drops.
The PCT kit contains 4 unique, preformulated, sterile filtered reagents used to evaluate protein concentra-
tion for crystallization screening. Initially, the sample protein is mixed with two of the reagents to determine
if the protein concentration is appropriate for crystallization screening. If the protein is very sensitive to salt
and polymer concentration, based on initial PCT results, the protein may be evaluated using a second set
of PCT reagents. PCT results will then provide insight to either the appropriate sample concentration or
indicate that other diagnostic testing such as native gel electrophoresis or dynamic light scattering should
be performed to demonstrate sample homogeneity appropriate for crystallization.
Each PCT kit contains 4 unique reagents, 50 ml each. This kit is useful for researchers who already have
crystallization plates and cover slides.
Each PCT kit (with plates) contains 4 unique reagents, 30 ml each, plus 24 well VDX Plates with sealant
and plain cover slides. This kit is useful for labs without access to crystallization plates and cover slides.
References
1. Crystallization and x-ray diffraction analysis of human CLEC-2. Aleksandra A. Watson and Christopher A. OCallaghan. Acta Cryst. (2005). F61, 10941096
Order Information
Each PCT kit contains 4 unique reagents. To order individual reagents, use Custom Shop

catalog
number listed below. Refer to page 36 for further details.
Cat. No. Name Description Price
HR2-140 PCT 50 ml bottles (4 ea), plain cover slides (1 pk) $80.00
HR2-142 PCT (with Plates) 30 ml bottles (4 ea), plain cover slides (1 pk), $98.00
VDX Plates with sealant (5 ea)
HR2-940-** PCT Custom Shop 185 ml $138.00
** = reagent number A1-B2
d e s c r i p t i o n
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P C T

P R E - C R Y S T A L L I Z A T I O N T E S T
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 6
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s
n
Primary, diverse reagent system crystalliza-
tion screen for proteins, complexes, peptides,
nucleic acids, & water soluble small molecules
n
Developed at Hampton Research
n
A data-driven biased sparse matrix and grid
screen
n
Screens classic, contemporary, & modern
crystallization reagents
n
Samples pH 3 to 9
n
Compatible with microbatch, vapor diffusion,
& liquid diffusion methods
n
Specially formulated reagent zones:
n
Traditional salts versus pH
n
Neutralized organic acids
n
High [salt] with low [polymer]
n
High [polymer] with low [salt]
n
Low ionic strength versus pH
n
PEG & Salt versus pH
n
PEG & Salt
n
Tube or Deep Well block format
a p p l i c a t i o n
f e a t u r e s
Index is designed as a 96 reagent crystallization screen
that combines the strategies of the grid, sparse matrix,
and incomplete factorial screening with traditional, con-
temporary, and new crystallization reagent systems into a
highly effective and efficient format.
Index, as the name implies, efficiently samples a series
of specially formulated reagent zones to identify which reagent class or classes and pH are effective in
producing crystals or limiting sample solubility. Results from Index can be used to design optimization
experiments and to identify follow on screens by reagent class. For example, positive results with salt based
reagent in Index may be followed up with further screening using SaltRx or Grid Screen Salt. Success with
polymer based reagents in Index may be followed up with further screening using PEGRx or PEG/Ion.
Index utilizes a broad, yet refined portfolio of crystallization reagent systems. These include the following:
(1) traditional salts such as Ammonium sulfate and Sodium chloride versus pH; (2) neutralized organic acids
such as Sodium malonate and Tacsimate; (3) High salt concentration mixed with low polymer concentra-
tion as well as high polymer concentration mixed with low salt concentration and; (4) Low ionic strength
using polymers such as PEG, MPD, Pentaerythritols versus pH. These reagent systems are formulated
across a sparse matrix and incomplete factorial of concentration ranges, sampling a pH range of 3 to 9.
Index contains 96 unique reagents, 10 ml each.
Index HT contains 96 unique reagents in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest
purity salts, polymers, organics and buffers.
Measured pH range of kit is 3 to 9 at 25C
Average measured pH of kit is 6.8 at 25C
Median measured pH of kit is 6.9 at 25C
References
1. The advantages of using a modified microbatch method for rapid screening of protein crystallization conditions. A. D'Arcy, A. Mac Sweeney, M. Stihle and
A. Haber. Acta Cryst. (2003). D59, 396-399.
2. Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of propionate kinase (TdcD) from Salmonella typhimurium.
D. K. Simanshu and M. R. N. Murthy. Acta Cryst. (2005). F61, 52-55.
3. Preparation, crystallization and preliminary X-ray analysis of the methionine synthase (MetE) from Streptococcus mutans. T.-M. Fu, X.-Y. Zhang, L.-F. Li,
Y.-H. Liang and X.-D. Su. Acta Cryst. (2006). F62, 984-985.
d e s c r i p t i o n
I N D E X

I n d e x H T

Crystals of a diabetes related protein.


Courtesy of Allan DArcy and Aengus Mac Sweeney.
Morphochem AG
s u c c e s s s t o r y
Order Information
Each Index kit contains 96 unique reagents. To order individual reagents, use Custom Shop

cata-
log number listed below. Refer to page 36 for further details.
Cat. No. Name Description Price
HR2-144 Index 10 ml, tube format $570.00
HR2-134 Index HT 1 ml, Deep Well block format $185.00
HR2-944-** Index Custom Shop 185 ml $138.00

** = reagent number 1-96
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1. 0.1 M Citric Acid pH 3.5, 2.0 M Ammonium Sulfate
2. 0.1 M Sodium Acetate trihydrate pH 4.5, 2.0 M Ammonium Sulfate
3. 0.1 M Bis-Tris pH 5.5, 2.0 M Ammonium Sulfate
4. 0.1 M Bis-Tris pH 6.5, 2.0 M Ammonium Sulfate
5. 0.1 M HEPES pH 7.5, 2.0 M Ammonium Sulfate
6. 0.1 M Tris pH 8.5, 2.0 M Ammonium Sulfate
7. 0.1 M Citric Acid pH 3.5, 3.0 M Sodium Chloride
8. 0.1 M Sodium Acetate trihydrate pH 4.5, 3.0 M Sodium Chloride
9. 0.1 M Bis-Tris pH 5.5, 3.0 M Sodium Chloride
10. 0.1 M Bis-Tris pH 6.5, 3.0 M Sodium Chloride
11. 0.1 M HEPES pH 7.5, 3.0 M Sodium Chloride
12. 0.1 M Tris pH 8.5, 3.0 M Sodium Chloride
13. 0.1 M Bis-Tris pH 5.5, 0.3 M Magnesium Formate
14. 0.1 M Bis-Tris pH 6.5, 0.5 M Magnesium Formate
15. 0.1 M HEPES pH 7.5, 0.5 M Magnesium Formate
16. 0.1 M Tris pH 8.5, 0.3 M Magnesium Formate
17. 1.4 M Sodium/Potassium Phosphate pH 5.6
18. 1.4 M Sodium/Potassium Phosphate pH 6.9
19. 1.4 M Sodium/Potassium Phosphate pH 8.2
20. 0.1 M HEPES pH 7.5, 1.4 M tri-Sodium Citrate dihydrate
21. 1.8 M tri-Ammonium Citrate pH 7.0
22. 0.8 M Succinic Acid pH 7.0
23. 2.1 M DL-Malic Acid pH 7.0
24. 2.8 M Sodium Acetate trihydrate pH 7.0
25. 3.5 M Sodium Formate pH 7.0
26. 1.1 M di-Ammonium Tartrate pH 7.0
27. 2.4 M Sodium Malonate pH 7.0
28. 35% v/v Tacsimate pH 7.0
29. 60% v/v Tacsimate pH 7.0
30. 0.1 M Sodium Chloride, 0.1 M Bis-Tris pH 6.5, 1.5 M Ammonium Sulfate
31. 0.8 M Potassium Sodium Tartrate tetrahydrate, 0.1 M Tris pH 8.5, 0.5% w/v Polyethylene Glycol Monomethyl ether 5000
32. 1.0 M Ammonium Sulfate, 0.1 M Bis-Tris pH 5.5, 1% w/v Polyethylene Glycol 3350
33. 1.1 M Sodium Malonate pH 7.0, 0.1 M HEPES pH 7.0, 0.5% v/v Jeffamine ED-2001 Reagent pH 7.0
34. 1.0 M Succinic Acid pH 7.0, 0.1 M HEPES pH 7.0, 1% w/v Polyethylene Glycol Monomethyl ether 2000
35. 1.0 M Ammonium Sulfate, 0.1 M HEPES pH 7.0, 0.5% w/v Polyethylene Glycol 8000
36. 15% v/v Tacsimate pH 7.0, 0.1 M HEPES pH 7.0, 2% w/v Polyethylene Glycol 3350
37. 25% w/v Polyethylene Glycol 1500
38. 0.1 M HEPES pH 7.0, 30% v/v Jeffamine M-600 Reagent pH 7.0
39. 0.1 M HEPES pH 7.0, 30% v/v Jeffamine ED-2001 Reagent pH 7.0
40. 0.1 M Citric Acid pH 3.5, 25% w/v Polyethylene Glycol 3350
41. 0.1 M Sodium Acetate trihydrate pH 4.5, 25% w/v Polyethylene Glycol 3350
42. 0.1 M Bis-Tris pH 5.5, 25% w/v Polyethylene Glycol 3350
43. 0.1 M Bis-Tris pH 6.5, 25% w/v Polyethylene Glycol 3350
44. 0.1 M HEPES pH 7.5, 25% w/v Polyethylene Glycol 3350
45. 0.1 M Tris pH 8.5, 25% w/v Polyethylene Glycol 3350
46. 0.1 M Bis-Tris pH 6.5, 20% w/v Polyethylene Glycol Monomethyl ether 5000
47. 0.1 M Bis-Tris pH 6.5, 28% w/v Polyethylene Glycol Monomethyl ether 2000
48. 0.2 M Calcium Chloride dihydrate, 0.1 M Bis-Tris pH 5.5, 45% v/v 2-Methyl-2,4-pentanediol
49. 0.2 M Calcium Chloride dihydrate, 0.1 M Bis-Tris pH 6.5, 45% v/v 2-Methyl-2,4-pentanediol
50. 0.2 M Ammonium Acetate, 0.1 M Bis-Tris pH 5.5, 45% v/v 2-Methyl-2,4-pentanediol
51. 0.2 M Ammonium Acetate, 0.1 M Bis-Tris pH 6.5, 45% v/v 2-Methyl-2,4-pentanediol
52. 0.2 M Ammonium Acetate, 0.1 M HEPES pH 7.5, 45% v/v 2-Methyl-2,4-pentanediol
53. 0.2 M Ammonium Acetate, 0.1 M Tris pH 8.5, 45% v/v 2-Methyl-2,4-pentanediol
54. 0.05 M Calcium Chloride dihydrate, 0.1 M Bis-Tris pH 6.5, 30% v/v Polyethylene Glycol Monomethyl ether 550
55. 0.05 M Magnesium Chloride hexahydrate, 0.1 M HEPES pH 7.5, 30% v/v Polyethylene Glycol Monomethyl ether 550
56. 0.2 M Potassium Chloride, 0.05 M HEPES pH 7.5, 35% v/v Pentaerythritol Propoxylate (5/4 PO/OH)
57. 0.05 M Ammonium Sulfate, 0.05 M Bis-Tris pH 6.5, 30% v/v Pentaerythritol Ethoxylate (15/4 EO/OH)
58. 0.1 M Bis-Tris pH 6.5, 45% v/v Polypropylene Glycol P 400
59. 0.02 M Magnesium Chloride hexahydrate, 0.1 M HEPES pH 7.5, 22% w/v Polyacrylic Acid 5100 Sodium salt
60. 0.01 M Cobalt Chloride hexahydrate, 0.1 M Tris pH 8.5, 20% w/v Polyvinylpyrrolidone K15
61. 0.2 M Proline, 0.1 M HEPES pH 7.5, 10% w/v Polyethylene Glycol 3350
62. 0.2 M Trimethylamine N-oxide dihydrate, 0.1 M Tris pH 8.5, 20% w/v Polyethylene Glycol Monomethyl ether 2000
63. 5% v/v Tacsimate pH 7.0, 0.1 M HEPES pH 7.0, 10% w/v Polyethylene Glycol Monomethyl ether 5000
64. 0.005 M Cobalt Chloride hexahydrate, 0.005 M Nickel (II) Chloride hexahydrate, 0.005 M Cadmium Chloride dihydrate,
0.005 M Magnesium Chloride hexahydrate, 0.1 M HEPES pH 7.5, 12% w/v Polyethylene Glycol 3350
65. 0.1 M Ammonium Acetate, 0.1 M Bis-Tris pH 5.5, 17% w/v Polyethylene Glycol 10,000
66. 0.2 M Ammonium Sulfate, 0.1 M Bis-Tris pH 5.5, 25% w/v Polyethylene Glycol 3350
67. 0.2 M Ammonium Sulfate, 0.1 M Bis-Tris pH 6.5, 25% w/v Polyethylene Glycol 3350
68. 0.2 M Ammonium Sulfate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene Glycol 3350
69. 0.2 M Ammonium Sulfate, 0.1 M Tris pH 8.5, 25% w/v Polyethylene Glycol 3350
70. 0.2 M Sodium Chloride, 0.1 M Bis-Tris pH 5.5, 25% w/v Polyethylene Glycol 3350
71. 0.2 M Sodium Chloride, 0.1 M Bis-Tris pH 6.5, 25% w/v Polyethylene Glycol 3350
72. 0.2 M Sodium Chloride, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene Glycol 3350
73. 0.2 M Sodium Chloride, 0.1 M Tris pH 8.5, 25% w/v Polyethylene Glycol 3350
74. 0.2 M Lithium Sulfate monohydrate, 0.1 M Bis-Tris pH 5.5, 25% w/v Polyethylene Glycol 3350
75. 0.2 M Lithium Sulfate monohydrate, 0.1 M Bis-Tris pH 6.5, 25% w/v Polyethylene Glycol 3350
76. 0.2 M Lithium Sulfate monohydrate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene Glycol 3350
77. 0.2 M Lithium Sulfate monohydrate, 0.1 M Tris pH 8.5, 25% w/v Polyethylene Glycol 3350
78. 0.2 M Ammonium Acetate, 0.1 M Bis-Tris pH 5.5, 25% w/v Polyethylene Glycol 3350
79. 0.2 M Ammonium Acetate, 0.1 M Bis-Tris pH 6.5, 25% w/v Polyethylene Glycol 3350
80. 0.2 M Ammonium Acetate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene Glycol 3350
81. 0.2 M Ammonium Acetate, 0.1 M Tris pH 8.5, 25% w/v Polyethylene Glycol 3350
82. 0.2 M Magnesium Chloride hexahydrate, 0.1 M Bis-Tris pH 5.5, 25% w/v Polyethylene Glycol 3350
83. 0.2 M Magnesium Chloride hexahydrate, 0.1 M Bis-Tris pH 6.5, 25% w/v Polyethylene Glycol 3350
84. 0.2 M Magnesium Chloride hexahydrate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene Glycol 3350
85. 0.2 M Magnesium Chloride hexahydrate, 0.1 M Tris HCl pH 8.5, 25% w/v Polyethylene Glycol 3350
86. 0.2 M Potassium Sodium Tartrate tetrahydrate, 20% w/v Polyethylene Glycol 3350
87. 0.2 M Sodium Malonate pH 7.0, 20% w/v Polyethylene Glycol 3350
88. 0.2 M tri-Ammonium Citrate pH 7.0, 20% w/v Polyethylene Glycol 3350
89. 0.1 M Succinic Acid pH 7.0, 15% w/v Polyethylene Glycol 3350
90. 0.2 M Sodium Formate, 20% w/v Polyethylene Glycol 3350
91. 0.15 M DL-Malic Acid pH 7.0, 20% w/v Polyethylene Glycol 3350
92. 0.1 M Magnesium Formate, 15% w/v Polyethylene Glycol 3350
93. 0.05 M Zinc Acetate dihydrate, 20% w/v Polyethylene Glycol 3350
94. 0.2 M tri-Sodium Citrate dihydrate, 20% w/v Polyethylene Glycol 3350
95. 0.1 M Potassium Thiocyanate, 30% w/v Polyethylene Glycol Monomethyl ether 2000
96. 0.15 M Potassium Bromide, 30% w/v Polyethylene Glycol Monomethyl ether 2000
I N D E X F O R M U L A T I O N
A1
A2
A3
A4
A5
A6
A7
A8
A9
A10
A11
A12
B1
B2
B3
B4
B5
B6
B7
B8
B9
B10
B11
B12
C1
C2
C3
C4
C5
C6
C7
C8
C9
C10
C11
C12
D1
D2
D3
D4
D5
D6
D7
D8
D9
D10
D11
D12
E1
E2
E3
E4
E5
E6
E7
E8
E9
E10
E11
E12
F1
F2
F3
F4
F5
F6
F7
F8
F9
F10
F11
F12
G1
G2
G3
G4
G5
G6
G7
G8
G9
G10
G11
G12
H1
H2
H3
H4
H5
H6
H7
H8
H9
H10
H11
H12
A1
A2
A3
A4
A5
A6
A7
A8
A9
A10
A11
A12
B1
B2
B3
B4
B5
B6
B7
B8
B9
B10
B11
B12
C1
C2
C3
C4
C5
C6
C7
C8
C9
C10
C11
C12
D1
D2
D3
D4
D5
D6
D7
D8
D9
D10
D11
D12
E1
E2
E3
E4
E5
E6
E7
E8
E9
E10
E11
E12
F1
F2
F3
F4
F5
F6
F7
F8
F9
F10
F11
F12
G1
G2
G3
G4
G5
G6
G7
G8
G9
G10
G11
G12
H1
H2
H3
H4
H5
H6
H7
H8
H9
H10
H11
H12
Range from 3 to 9
p
H
h i g h [ p o l y m e r ] /
l o w [ s a l t ]
n e u t r a l i z e d
o r g a n i c a c i d s
t r a d i t i o n a l
s a l t s
p o l y m e r /
s a l t
i n d e x
f a c t o r s
h i g h [ s a l t ] /
l o w [ p o l y m e r ]
p o l y m e r / s a l t / p H
l o w i o n i c
s t r e n g t h
n
Primary screen for proteins, soluble peptides,
nucleic acids, & water soluble small molecules
n
Sparse matrix additive screen
n
The original sparse matrix screen
n
Sparse matrix formula efficiently samples
salts, polymers, organics, & pH
n
Proven effective with more than 1,000
biological macromolecules
n
Tube or DeepWell block format
a p p l i c a t i o n
f e a t u r e s
The Crystal Screen and Crystal Screen 2 reagent
kits are designed to provide a highly effective and
rapid screening method for the crystallization
of macromolecules. The screens are simple and
practical for finding initial crystallization condi-
tions. The initial crystallization conditions for
more than 1,000 proteins, peptides, oligonucle-
otides, and small molecules have been deter-
mined using Crystal Screen.
A highly effective approach to overcome the exhaustive search for suitable crystallization conditions is the
use of a sparse matrix method of trial conditions that is biased and selected from known crystallization
conditions for macromolecules. The formulation utilized in Crystal Screen and Crystal Screen 2 evaluates
96 unique mixtures of pH, salts, polymers and organics, and their ability to promote crystal growth.
Crystal Screen contains 50 unique reagents, 10 ml each and is based on the sparse matrix formulation first
described by Jancarik and Kim in 1991.
Crystal Screen 2, an extension of Crystal Screen, contains 48 unique reagents, 10 ml each and is based on
the formulation first described by Cudney et al in 1994.
Crystal Screen HT contains 1 ml each of reagents 1-48 from Crystal Screen and all 48 reagents from Crystal
Screen 2 in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest
purity salts, polymers, organics and buffers.
References
1. Jancarik, J. & Kim, S.H. J. Appl. Cryst. 24, 409-411, (1991).
2. Expression, purification, crystallization and preliminary X-ray analysis of two arginine-biosynthetic enzymes from Mycobacterium tuberculosis.
F. Moradian, C. Garen, L. Cherney, M. Cherney and M. N. G. James. Acta Cryst. (2006). F62, 986-988.
d e s c r i p t i o n
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 8
Crystal of gp120, the exterior envelope glycoprotein
of type 1 HIV. Preliminary crystallization conditions
obtained using Crystal Screen from Hampton Research.
Courtesy of P.D. Kwong
1
, R. Wyatt
2
, E. Desjardins
2
,
J. Robinson
3
, F.C. Culp
4
, B.D. Hellmig
4
, R.W. Sweet
4
,
J. Sodroski
2
, and W.A. Hendrickson
1,5
.
1
Columbia University,
2
Dana-Farber Cancer Institute,
3
Tulane
University School of Medicine,
4
GlaxoSmithKline ,
5
HHMI-
Columbia University
s u c c e s s s t o r y
CRYSTAL SCREEN

CrysTal sCreen 2

CrysTal sCreen

HT

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Order Information
Each Crystal Screen kit contains 50 unique reagents. Each Crystal Screen 2 kit contains 48 unique
reagents. To order individual reagents, use Custom Shop

catalog numbers listed below. Refer to


page 36 for further details.
Cat. No. Name Description Price
HR2-110 Crystal Screen 10 ml, tube format $285.00
HR2-112 Crystal Screen 2 10 ml, tube format $285.00
HR2-130 Crystal Screen HT 1 ml, Deep Well block format $185.00
HR2-910-** Crystal Screen Custom Shop 185 ml $138.00
HR2-912-** Crystal Screen 2 Custom Shop 185 ml $138.00

** = reagent number 1-50 (for Crystal Screen)
** = reagent number 1-48 (for Crystal Screen 2)
CRYSTAL SCREEN

CrysTal sCreen 2

CrysTal sCreen

HT

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B1
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B3
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B6
B7
B8
B9
B10
B11
B12
C1
C2
C3
C4
C5
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C7
C8
C9
C10
C11
C12
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H3
H4
H5
H6
H7
H8
H9
H10
H11
H12
C R Y S T A L S C R E E N F O R M U L A T I O N
1. 0.02 M Calcium chloride dihydrate, 0.1 M Sodium acetate trihydrate pH 4.6, 30% v/v (+/-)-2-Methyl-2,4-pentanediol
2. 0.4 M Potassium sodium tartrate tetrahydrate
3. 0.4 M Ammonium phosphate monobasic
4. 0.1 M TRIS hydrochloride pH 8.5, 2.0 M Ammonium sulfate
5. 0.2 M Sodium citrate tribasic dihydrate, 0.1 M HEPES sodium pH 7.5, 30% v/v (+/-)-2-Methyl-2,4-pentanediol
6. 0.2 M Magnesium chloride hexahydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% w/v Polyethylene glycol 4,000
7. 0.1 M Sodium cacodylate trihydrate pH 6.5, 1.4 M Sodium acetate trihydrate
8. 0.2 M Sodium citrate tribasic dihydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 30% v/v 2-Propanol
9. 0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 30% w/v Polyethylene glycol 4,000
10. 0.2 M Ammonium acetate, 0.1 M Sodium acetate trihydrate pH 4.6, 30% w/v Polyethylene glycol 4,000
11. 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 1.0 M Ammonium phosphate monobasic
12. 0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES sodium pH 7.5, 30% v/v 2-Propanol
13. 0.2 M Sodium citrate tribasic dihydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% v/v Polyethylene glycol 400
14. 0.2 M Calcium chloride dihydrate, 0.1 M HEPES sodium pH 7.5, 28% v/v Polyethylene glycol 400
15. 0.2 M Ammonium sulfate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 30% w/v Polyethylene glycol 8,000
16. 0.1 M HEPES sodium pH 7.5, 1.5 M Lithium sulfate monohydrate
17. 0.2 M Lithium sulfate monohydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% w/v Polyethylene glycol 4,000
18. 0.2 M Magnesium acetate tetrahydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 20% w/v Polyethylene glycol 8,000
19. 0.2 M Ammonium acetate, 0.1 M TRIS hydrochloride pH 8.5, 30% v/v 2-Propanol
20. 0.2 M Ammonium sulfate, 0.1 M Sodium acetate trihydrate pH 4.6, 25% w/v Polyethylene glycol 4,000
21. 0.2 M Magnesium acetate tetrahydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 30% v/v (+
/
-)-2-Methyl-2,4-pentanediol
22. 0.2 M Sodium acetate trihydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% w/v Polyethylene glycol 4,000
23. 0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES sodium pH 7.5, 30% v/v Polyethylene glycol 400
24. 0.2 M Calcium chloride dihydrate, 0.1 M Sodium acetate trihydrate pH 4.6, 20% v/v 2-Propanol
25. 0.1 M Imidazole pH 6.5, 1.0 M Sodium acetate trihydrate
26. 0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 30% v/v (+
/
-)-2-Methyl-2,4-pentanediol
27. 0.2 M Sodium citrate tribasic dihydrate, 0.1 M HEPES sodium pH 7.5, 20% v/v 2-Propanol
28. 0.2 M Sodium acetate trihydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 30% w/v Polyethylene glycol 8,000
29. 0.1 M HEPES sodium pH 7.5, 0.8 M Potassium sodium tartrate tetrahydrate
30. 0.2 M Ammonium sulfate, 30% w/v Polyethylene glycol 8,000
31. 0.2 M Ammonium sulfate, 30% w/v Polyethylene glycol 4,000
32. 2.0 M Ammonium sulfate
33. 4.0 M Sodium formate
34. 0.1 M Sodium acetate trihydrate pH 4.6, 2.0 M Sodium formate
35. 0.1 M HEPES sodium pH 7.5, 0.8 M Sodium phosphate monobasic monohydrate, 0.8 M Potassium phosphate monobasic
36. 0.1 M TRIS hydrochloride pH 8.5, 8% w/v Polyethylene glycol 8,000
37. 0.1 M Sodium acetate trihydrate pH 4.6, 8% w/v Polyethylene glycol 4,000
38. 0.1 M HEPES sodium pH 7.5, 1.4 M Sodium citrate tribasic dihydrate
39. 0.1 M HEPES sodium pH 7.5, 2% v/v Polyethylene glycol 400, 2.0 M Ammonium sulfate
40. 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 20% v/v 2-Propanol, 20% w/v Polyethylene glycol 4,000
41. 0.1 M HEPES sodium pH 7.5, 10% v/v 2-Propanol, 20% w/v Polyethylene glycol 4,000
42. 0.05 M Potassium phosphate monobasic, 20% w/v Polyethylene glycol 8,000
43. 30% w/v Polyethylene glycol 1,500
44. 0.2 M Magnesium formate dihydrate
45. 0.2 M Zinc acetate dihydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 18% w/v Polyethylene glycol 8,000
46. 0.2 M Calcium acetate hydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5 , 18% w/v Polyethylene glycol 8,000
47. 0.1 M Sodium acetate trihydrate pH 4.6, 2.0 M Ammonium sulfate
48. 0.1 M TRIS hydrochloride pH 8.5, 2.0 M Ammonium phosphate monobasic
49. 1.0 M Lithium sulfate monohydrate, 2% w/v Polyethylene glycol 8,000
50. 0.5 M Lithium sulfate monohydrate, 15% w/v Polyethylene glycol 8,000
1. 2.0 M Sodium chloride, 10% w/v Polyethylene glycol 6,000
2. 0.5 M Sodium chloride, 0.01 M Magnesium chloride hexahydrate, 0.01 M Hexadecyltrimethylammonium bromide
3. 25% v/v Ethylene glycol
4. 35% v/v 1,4-Dioxane
5. 2.0 M Ammonium sulfate, 5% v/v 2-Propanol
6. 1.0 M Imidazole pH 7.0
7. 10% w/v Polyethylene glycol 1,000, 10% w/v Polyethylene glycol 8,000
8. 1.5 M Sodium chloride, 10% v/v Ethanol
9. 0.1 M Sodium acetate trihydrate pH 4.6, 2.0 M Sodium chloride
10. 0.2 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 4.6, 30% v/v (+
/
-)-2-Methyl-2,4-pentanediol
11. 0.01 M Cobalt(II) chloride hexahydrate, 0.1 M Sodium acetate trihydrate pH 4.6, 1.0 M 1,6-Hexanediol
12. 0.1 M Cadmium chloride hydrate, 0.1 M Sodium acetate trihydrate pH 4.6, 30% v/v Polyethylene glycol 400
13. 0.2 M Ammonium sulfate, 0.1 M Sodium acetate trihydrate pH 4.6, 30% w/v Polyethylene glycol monomethyl ether 2,000
14. 0.2 M Potassium sodium tartrate tetrahydrate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 2.0 M Ammonium sulfate
15. 0.5 M Ammonium sulfate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 1.0 M Lithium sulfate monohydrate
16. 0.5 M Sodium chloride, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 2% v/v Ethylene imine polymer
17. 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 35% v/v tert-Butanol
18. 0.01 M Iron(III) chloride hexahydrate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 10% v/v Jeffamine M-600
19. 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 2.5 M 1,6-Hexanediol
20. 0.1 M MES monohydrate pH 6.5, 1.6 M Magnesium sulfate heptahydrate
21. 0.1 M Sodium phosphate monobasic monohydrate, 0.1 M Potassium phosphate monobasic,
0.1 M MES monohydrate pH 6.5, 2.0 M Sodium chloride
22. 0.1 M MES monohydrate pH 6.5, 12% w/v Polyethylene glycol 20,000
23. 1.6 M Ammonium sulfate, 0.1 M MES monohydrate pH 6.5, 10% v/v 1,4-Dioxane
24. 0.05 M Cesium chloride, 0.1 M MES monohydrate pH 6.5, 30% v/v Jeffamine M-600
25. 0.01 M Cobalt(II) chloride hexahydrate, 0.1 M MES monohydrate pH 6.5, 1.8 M Ammonium sulfate
26. 0.2 M Ammonium sulfate, 0.1 M MES monohydrate pH 6.5, 30% w/v Polyethylene glycol monomethyl ether 5,000
27. 0.01 M Zinc sulfate heptahydrate, 0.1 M MES monohydrate pH 6.5, 25% v/v Polyethylene glycol monomethyl ether 550
28. 1.6 M Sodium citrate tribasic dihydrate pH 6.5
29. 0.5 M Ammonium sulfate, 0.1 M HEPES pH 7.5, 30% v/v (+
/
-)-2-Methyl-2,4-pentanediol
30. 0.1 M HEPES pH 7.5, 10% w/v Polyethylene glycol 6,000, 5% v/v (+
/
-)-2-Methyl-2,4-pentanediol
31. 0.1 M HEPES pH 7.5, 20% v/v Jeffamine M-600
32. 0.1 M Sodium chloride, 0.1 M HEPES pH 7.5, 1.6 M Ammonium sulfate
33. 0.1 M HEPES pH 7.5, 2.0 M Ammonium formate
34. 0.05 M Cadmium sulfate hydrate, 0.1 M HEPES pH 7.5, 1.0 M Sodium acetate trihydrate
35. 0.1 M HEPES pH 7.5, 70% v/v (+
/
-)-2-Methyl-2,4-pentanediol
36. 0.1 M HEPES pH 7.5, 4.3 M Sodium chloride
37. 0.1 M HEPES pH 7.5, 10% w/v Polyethylene glycol 8,000, 8% v/v Ethylene glycol
38. 0.1 M HEPES pH 7.5, 20% w/v Polyethylene glycol 10,000
39. 0.2 M Magnesium chloride hexahydrate, 0.1 M Tris pH 8.5, 3.4 M 1,6-Hexanediol
40. 0.1 M Tris pH 8.5, 25% v/v tert-Butanol
41. 0.01 M Nickel(II) chloride hexahydrate, 0.1 M Tris pH 8.5, 1.0 M Lithium sulfate monohydrate
42. 1.5 M Ammonium sulfate, 0.1 M Tris pH 8.5, 12% v/v Glycerol
43. 0.2 M Ammonium phosphate monobasic, 0.1 M Tris pH 8.5, 50% v/v (+
/
-)-2-Methyl-2,4-pentanediol
44. 0.1 M Tris pH 8.5, 20% v/v Ethanol
45. 0.01 M Nickel(II) chloride hexahydrate, 0.1 M Tris pH 8.5, 20% w/v Polyethylene glycol monomethyl ether 2,000
46. 0.1 M Sodium chloride, 0.1 M BICINE pH 9.0, 20% v/v Polyethylene glycol monomethyl ether 550
47. 0.1 M BICINE pH 9.0, 2.0 M Magnesium chloride hexahydrate
48. 0.1 M BICINE pH 9.0, 2% v/v 1,4-Dioxane, 10% w/v Polyethylene glycol 20,000
C R Y S T A L S C R E E N 2 F O R M U L A T I O N
Range from 4.0 to 9.0
1,4-Dioxane
Ethanol
2-Propanol
tert-Butanol
1,6-Hexanediol
(+/-)-2-Methyl-2,4-pentanediol
Ethylene glycol
Glycerol
Ethylene imine polymer
Jeffamine
M-600
Polyethylene glycol 400
Polyethylene glycol 1,000
Polyethylene glycol 6,000
Polyethylene glycol 8,000
Polyethylene glycol 10,000
Polyethylene glycol 20,000
Polyethylene glycol MME 550
Polyethylene glycol MME 2,000
Polyethylene glycol MME 5,000
BICINE
HEPES
Imidazole
MES
Sodium acetate
Sodium citrate
TRIS hydrochloride
o r g a n i c
p
H
n o n - v o l a t i l e
o r g a n i c
s a l t
p o l y m e r
b u f f e r
Ammonium phosphate
Potassium phosphate
Sodium phosphate
Ammonium sulfate
Cadmium sulfate
Lithium sulfate
Magnesium sulfate
Zinc sulfate
K/Na tartrate
Hexadecyltrimethylammonium bromide
Sodium acetate
Cadmium chloride
Cesium chloride
Cobalt(II) chloride
Iron(III) chloride
Magnesium chloride
Nickel(II) chloride
Sodium chloride
Sodium citrate
Ammonium formate
p o l y m e r
Range from 4.0 to 9.0
2-Propanol
(+/-)-2-Methyl-2,4-pentanediol
Polyethylene glycol 400
Polyethylene glycol 1,500
Polyethylene glycol 4,000
Polyethylene glycol 8,000
Ammonium acetate
Calcium acetate
Magnesium acetate
Sodium acetate
Zinc acetate
Calcium chloride
Magnesium chloride
Sodium citrate
Magnesium formate
Sodium formate
Ammonium phosphate
Potassium phosphate
Sodium phosphate
Ammonium sulfate
Lithium sulfate
K/Na tartrate
o r g a n i c
p
H
n o n - v o l a t i l e
o r g a n i c
s a l t
HEPES sodium
Imidazole
Sodium acetate
Sodium cacodylate
Sodium citrate
TRIS hydrochloride
b u f f e r
crystal screen
factors
crystal screen 2
factors
n
Primary and secondary, polymer and pH
based crystallization screen for biological
macromolecules
n
Developed at Hampton Research
n
PEGRx 1 primary screen variables are
polymer type (16 different polymers), polymer
molecular weight, pH and low ionic strength.
n
PEGRx 2 primary screen variables are
polymer type (13 different polymers), polymer
molecular weight, pH and secondary reagents
which include additives, salts, volatile
organics and polyols.
n
PEGRx HT combines PEGRx 1 and PEGRx 2
in a single 96 Deep Well block
n
pH range 3.5 - 9, using 10 different buffer
systems
n
Polymer molecular weight range 200 to
20,000
n
Tube or Deep Well block format
a p p l i c a t i o n
f e a t u r e s
PEGRx is a primary and secondary, polymer
and pH based crystallization screen devel-
oped at Hampton Research. It is
designed to evaluate polymer based crystal-
lization reagents and pH in low (PEGRx 1) to
medium ionic strength (PEGRx 2). It is also
designed for use as a secondary or optimiza-
tion screen to follow the Hampton Research
Index screen when polymer based reagents
produce hits and interesting solubility leads.
PEGRx 1 is a crystallization reagent kit designed to evaluate an array of polymers of varying molecu-
lar weight in a low ionic strength environment versus a wide range of pH. Polymer reagents include
Polyethylene glycols, Polyethylene glycol monomethylethers, and Jeffamines. The molecular weight range
between 200 and 20,000 is evaluated in a low ionic strength formulation. Ten different buffers are used to
span the range of pH between 3.5 and 9. The primary screen variables are polymer type, polymer molecular
weight, pH and low ionic strength.
PEGRx 2 is a crystallization reagent kit designed to evaluate an array of polymers of varying molecular
weight in a medium ionic strength environment in the presence of additives, salts, volatile organics and
polyols versus a wide range of pH. Polymer reagents include Polyethylene glycols and Polyethylene gly-
col monomethylethers. The polymer molecular weight range between 200 and 20,000 is evaluated in a
medium ionic strength formulation. Ten different buffers are used to span the range of pH between 3.5 and
9. The primary screen variables are polymer type, polymer molecular weight, pH and secondary reagents
which include additives, salts, volatile organics and polyols.
PEGRx 1 contains 48 unique reagents, 10 ml each.
PEGRx 2 contains 48 unique reagents, 10 ml each.
PEGRx HT contains 1 ml of each reagent from PEGRx 1 and PEGRx 2 in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest
purity salts, polymers, organics and buffers.
d e s c r i p t i o n
Order Information
PEGRx 1 and PEGRx 2 kits each contain 48 unique reagents. To order individual reagents, use
Custom Shop

catalog numbers listed below. Refer to page 36 for further details.


Cat. No. Name Description Price
HR2-082 PEGRx 1 10 ml, tube format $285.00
HR2-084 PEGRx 2 10 ml, tube format $285.00
HR2-086 PEGRx HT 1 ml, Deep Well block format $185.00
HR2-982-** PEGRx 1 Custom Shop 185 ml $138.00
HR2-984-** PEGRx 2 Custom Shop 185 ml $138.00
** = reagent number 1-48
P E G R X 1

P e G r x 2

P e G r x H T

Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 10
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A2
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A4
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A6
A7
A8
A9
A10
A11
A12
B1
B2
B3
B4
B5
B6
B7
B8
B9
B10
B11
B12
C1
C2
C3
C4
C5
C6
C7
C8
C9
C10
C11
C12
D1
D2
D3
D4
D5
D6
D7
D8
D9
D10
D11
D12
E1
E2
E3
E4
E5
E6
E7
E8
E9
E10
E11
E12
F1
F2
F3
F4
F5
F6
F7
F8
F9
F10
F11
F12
G1
G2
G3
G4
G5
G6
G7
G8
G9
G10
G11
G12
H1
H2
H3
H4
H5
H6
H7
H8
H9
H10
H11
H12
P E G R X 1 F O R M U L A T I O N
1. 0.1 M Citric acid pH 3.5, 34% v/v Polyethylene glycol 200
2. 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 38% v/v Polyethylene glycol 200
3 0.1 M HEPES pH 7.5, 42% v/v Polyethylene glycol 200
4. 0.1 M Sodium acetate trihydrate pH 4.5, 30% v/v Polyethylene glycol 300
5. 0.1 M BIS-TRIS pH 6.5, 25% v/v Polyethylene glycol 300
6. 0.1 M BICINE pH 8.5, 20% v/v Polyethylene glycol 300
7. 0.1 M Sodium acetate trihydrate pH 4.0, 15% v/v Polyethylene glycol 400
8. 0.1 M MES monohydrate pH 6.0, 22% v/v Polyethylene glycol 400
9. 0.1 M Tris pH 8.0, 30% v/v Polyethylene glycol 400
10. 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 30% v/v Polyethylene glycol monomethyl ether 550
11. 0.1 M Imidazole pH 7.0, 25% v/v Polyethylene glycol monomethyl ether 550
12. 0.1 M BIS-TRIS propane pH 9.0, 20% v/v Polyethylene glycol monomethyl ether 550
13. 0.1 M Sodium acetate trihydrate pH 4.0, 10% v/v Jeffamine

M-600

pH 7.0
14. 0.1 M MES monohydrate pH 6.0, 20% v/v Jeffamine

M-600

pH 7.0
15. 0.1 M Tris pH 8.0, 30% v/v Jeffamine

M-600

pH 7.0
16. 0.1 M Citric acid pH 3.5, 14% w/v Polyethylene glycol 1,000
17. 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 22% w/v Polyethylene glycol 1,000
18. 0.1 M HEPES pH 7.5, 30% w/v Polyethylene glycol 1,000
19. 0.1 M Sodium acetate trihydrate pH 4.5, 30% w/v Polyethylene glycol 1,500
20. 0.1 M BIS-TRIS pH 6.5, 20% w/v Polyethylene glycol 1,500
21. 0.1 M BICINE pH 8.5, 15% w/v Polyethylene glycol 1,500
22. 0.1 M Sodium acetate trihydrate pH 4.0, 10% w/v Polyethylene glycol monomethyl ether 2,000
23. 0.1 M MES monohydrate pH 6.0, 20% w/v Polyethylene glycol monomethyl ether 2,000
24. 0.1 M Tris pH 8.0, 30% w/v Polyethylene glycol monomethyl ether 2,000
25. 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 30% v/v Jeffamine

ED-2001 pH 7.0
26. 0.1 M Imidazole pH 7.0, 20% v/v Jeffamine

ED-2001 pH 7.0
27. 0.1 M BIS-TRIS propane pH 9.0, 10% v/v Jeffamine

ED-2001 pH 7.0
28. 0.1 M Citric acid pH 3.5, 25% w/v Polyethylene glycol 3,350
29. 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 18% w/v Polyethylene glycol 3,350
30. 0.1 M HEPES pH 7.0, 12% w/v Polyethylene glycol 3,350
31. 0.1 M Sodium acetate trihydrate pH 4.0, 10% w/v Polyethylene glycol 4,000
32. 0.1 M MES monohydrate pH 6.0, 14% w/v Polyethylene glycol 4,000
33. 0.1 M Tris pH 8.0, 28% w/v Polyethylene glycol 4,000
34. 0.1 M Sodium acetate trihydrate pH 4.5, 30% w/v Polyethylene glycol monomethyl ether 5,000
35. 0.1 M BIS-TRIS pH 6.5, 20% w/v Polyethylene glycol monomethyl ether 5,000
36. 0.1 M BICINE pH 8.5, 8% w/v Polyethylene glycol monomethyl ether 5,000
37. 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 10% w/v Polyethylene glycol 6,000
38. 0.1 M Imidazole pH 7.0, 20% w/v Polyethylene glycol 6,000
39. 0.1 M BIS-TRIS propane pH 9.0, 30% w/v Polyethylene glycol 6,000
40. 0.1 M Citric acid pH 3.5, 28% w/v Polyethylene glycol 8,000
41. 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 16% w/v Polyethylene glycol 8,000
42. 0.1 M HEPES pH 7.5, 4% w/v Polyethylene glycol 8,000
43. 0.1 M Sodium acetate trihydrate pH 4.5, 10% w/v Polyethylene glycol 10,000
44. 0.1 M BIS-TRIS pH 6.5, 16% w/v Polyethylene glycol 10,000
45. 0.1 M BICINE pH 8.5, 20% w/v Polyethylene glycol 10,000
46. 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 18% w/v Polyethylene glycol 20,000
47. 0.1 M Imidazole pH 7.0, 12% w/v Polyethylene glycol 20,000
48. 0.1 M BIS-TRIS propane pH 9.0, 8% w/v Polyethylene glycol 20,000
1. 0.8 M Lithium sulfate monohydrate, 0.1 M Sodium acetate trihydrate pH 4.0, 4% v/v Polyethylene glycol 200
2. 0.2 M Lithium sulfate monohydrate, 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 26% v/v Polyethylene glycol 200
3. 0.05 M Calcium chloride dihydrate, 0.1 M MES monohydrate pH 6.0, 45% v/v Polyethylene glycol 200
4. 28% v/v 2-Propanol, 0.1 M BIS-TRIS pH 6.5, 3% v/v Polyethylene glycol 200
5. 20% v/v Tacsimate pH 7.0, 0.1 M HEPES pH 7.5, 2% v/v Polyethylene glycol 200
6. 10% v/v 2-Propanol, 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 26% v/v Polyethylene glycol 400
7. 0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 24% v/v Polyethylene glycol 400
8. 0.2 M Ammonium sulfate, 0.1 M BIS-TRIS pH 6.5, 18% v/v Polyethylene glycol 400
9. 0.19 mM CYMAL

-7, 0.1 M HEPES pH 7.5, 40% v/v Polyethylene glycol 400


10. 6% v/v 2-Propanol, 0.1 M Sodium acetate trihydrate pH 4.5, 26% v/v Polyethylene glycol monomethyl ether 550
11. 1.8 M Ammonium sulfate, 0.1 M BIS-TRIS pH 6.5, 2% v/v Polyethylene glycol monomethyl ether 550
12. 0.15 M DL-Malic acid pH 7.0, 0.1 M Imidazole pH 7.0, 22% v/v Polyethylene glycol monomethyl ether 550
13. 0.1 M Succinic acid pH 7.0, 0.1 M BICINE pH 8.5, 30% v/v Polyethylene glycol monomethyl ether 550
14. 0.1 M Lithium sulfate monohydrate, 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 20% w/v Polyethylene glycol 1,000
15. 0.1 M Sodium malonate pH 8.0, 0.1 M Tris pH 8.0, 30% w/v Polyethylene glycol 1,000
16. 4% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.1 M Citric acid pH 3.5, 20% w/v Polyethylene glycol 1,500
17. 0.2 M L-Proline, 0.1 M HEPES pH 7.5, 24% w/v Polyethylene glycol 1,500
18. 10% v/v 2-Propanol, 0.1 M BICINE pH 8.5, 30% w/v Polyethylene glycol 1,500
19. 0.1 M Sodium chloride, 0.1 M BIS-TRIS propane pH 9.0, 25% w/v Polyethylene glycol 1,500
20. 0.02 M Nickel(II) chloride hexahydrate, 0.02 M Magnesium chloride hexahydrate, 0.02 M Cadmium chloride hydrate,
0.1 M Sodium acetate trihydrate pH 4.5, 24% w/v Polyethylene glycol monomethyl ether 2,000
21. 20% v/v 2-Propanol, 0.1 M MES monohydrate pH 6.0, 20% w/v Polyethylene glycol monomethyl ether 2,000
22. 0.2 M Ammonium citrate tribasic pH 7.0, 0.1 M Imidazole pH 7.0, 20% w/v Polyethylene glycol monomethyl ether 2,000
23. 4.0 M Potassium formate, 0.1 M BIS-TRIS propane pH 9.0, 2% w/v Polyethylene glycol monomethyl ether 2,000
24. 50% v/v Tacsimate pH 4.0, 0.1 M Sodium acetate trihydrate pH 4.5, 1% w/v Polyethylene glycol 3,350
25. 0.10% w/v n-Octyl-b-D-glucoside, 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 22% w/v Polyethylene glycol 3,350
26. 2% v/v Tacsimate pH 7.0, 5% v/v 2-Propanol, 0.1 M Imidazole pH 7.0, 8% w/v Polyethylene glycol 3,350
27. 2% v/v 1,4-Dioxane, 0.1 M Tris pH 8.0, 15% w/v Polyethylene glycol 3,350
28. 18% v/v 2-Propanol, 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 20% w/v Polyethylene glycol 4,000
29. 6% v/v Tacsimate pH 6.0, 0.1 M MES monohydrate pH 6.0, 25% w/v Polyethylene glycol 4,000
30. 0.2 M Magnesium formate dihydrate, 0.1 M Sodium acetate trihydrate pH 4.0,
18% w/v Polyethylene glycol monomethyl ether 5,000
31. 2% v/v Polyethylene glycol 400, 0.1 M Imidazole pH 7.0, 24% w/v Polyethylene glycol monomethyl ether 5,000
32. 0.2 M Sodium formate, 0.1 M BICINE pH 8.5, 20% w/v Polyethylene glycol monomethyl ether 5,000
33. 4% v/v 2-Propanol, 0.1 M BIS-TRIS propane pH 9.0, 20% w/v Polyethylene glycol monomethyl ether 5,000
34. 6% v/v Ethylene glycol, 0.1 M Citric acid pH 3.5, 10% w/v Polyethylene glycol 6,000
35. 0.15 M Lithium sulfate monohydrate, 0.1 M Citric acid pH 3.5, 18% w/v Polyethylene glycol 6,000
36. 10% v/v 2-Propanol, 0.1 M Sodium acetate trihydrate pH 4.0, 22% w/v Polyethylene glycol 6,000
37. 0.2 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 4.0, 22% w/v Polyethylene glycol 8,000
38. 20% v/v 2-Propanol, 0.1 M Tris pH 8.0, 5% w/v Polyethylene glycol 8,000
39. 10% v/v Polyethylene glycol 200, 0.1 M BIS-TRIS propane pH 9.0, 18% w/v Polyethylene glycol 8,000
40. 15% v/v 2-Propanol, 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 10% w/v Polyethylene glycol 10,000
41. 0.4 M Sodium malonate pH 6.0, 0.1 M MES monohydrate pH 6.0, 0.5% w/v Polyethylene glycol 10,000
42. 0.2 M Potassium sodium tartrate tetrahydrate, 0.1 M BIS-TRIS pH 6.5, 10% w/v Polyethylene glycol 10,000
43. 5% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.1 M HEPES pH 7.5, 10% w/v Polyethylene glycol 10,000
44. 0.2 M Ammonium acetate, 0.1 M Tris pH 8.0, 16% w/v Polyethylene glycol 10,000
45. 5% v/v 2-Propanol, 0.1 M Citric acid pH 3.5, 6% w/v Polyethylene glycol 20,000
46. 1.0 M Sodium malonate pH 5.0, 0.1 M Sodium acetate trihydrate pH 4.5, 2% w/v Polyethylene glycol 20,000
47. 0.2 M Magnesium chloride hexahydrate, 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 10% w/v Polyethylene glycol 20,000
48. 3% w/v Dextran sulfate sodium salt, 0.1 M BICINE pH 8.5, 15% w/v Polyethylene glycol 20,000
P E G R X 2 F O R M U L A T I O N
p o l y m e r
Range from 3.5 to 9
BICINE
BIS-TRIS
BIS-TRIS propane
Citric acid
HEPES
Imidazole
MES monohydrate
Sodium acetate
Sodium citrate
Tris
Jeffamine

ED-2001
Jeffamine

M-600

Polyethylene glycol 200


Polyethylene glycol 300
Polyethylene glycol 400
Polyethylene glycol 1,000
Polyethylene glycol 1,500
Polyethylene glycol 3,350
Polyethylene glycol 4,000
Polyethylene glycol 6,000
Polyethylene glycol 8,000
Polyethylene glycol 10,000
Polyethylene glycol 20,000
Polyethylene glycol MME 550
Polyethylene glycol MME 2,000
Polyethylene glycol MME 5,000
p
H
b u f f e r s
s e c o n d a r y
r e a g e n t s
b u f f e r s
p o l y m e r s
BICINE
BIS-TRIS
BIS-TRIS propane
Citric acid
HEPES
Imidazole
MES monohydrate
Sodium acetate
Sodium citrate
Tris
Range from 3.5 to 9
p
H
Polyethylene glycol 200
Polyethylene glycol 400
Polyethylene glycol 1,000
Polyethylene glycol 1,500
Polyethylene glycol 3,350
Polyethylene glycol 4,000
Polyethylene glycol 6,000
Polyethylene glycol 8,000
Polyethylene glycol 10,000
Polyethylene glycol 20,000
Polyethylene glycol MME 550
Polyethylene glycol MME 2,000
Polyethylene glycol MME 5,000
Additive
Cation
Detergent
Organic Acid
Polyol
Volatile Organic
p e g r x 1
f a c t o r s
p e g r x 2
f a c t o r s
n
Primary or secondary, polymer, salt and pH
matrix crystallization screen for biological
macromolecules
n
Developed at Hampton Research
n
PEG/Ion is a sparse matrix profile of anions
and cations in the presence of monodisperse
Polyethylene glycol 3,350 over pH 4.5 - 9.2
n
PEG/Ion 2 screens a complete profile of
titrated organic acids at varying pH levels
(3.7 - 8.8) in the presence of monodisperse
PEG 3,350
n
PEG/Ion HT combines PEG/Ion and PEG/Ion
2 in a single 96 Deep Well block
n
Tube or Deep Well block format
a p p l i c a t i o n
f e a t u r e s
PEG/Ion, developed by Hampton Research,
is a crystallization screen designed to evalu-
ate monodisperse, high purity Polyethylene
glycol 3,350 and 48 unique salts represent-
ing a very complete range of anions and
cations frequently used in the crystallization
of biological macromolecules. The primary
screening variables are PEG, ion type, ionic
strength, and pH. More than 60% of the
published crystallizations utilized PEG as a
primary crystallization reagent and in approx-
imately 50% of those reports, the PEG was combined with an ion as a secondary crystallization reagent.
PEG/Ion 2 is an extension to the fundamental crystallization strategy in PEG/Ion. PEG/Ion 2 reagents cover
the monodisperse, high purity Polyethylene glycol 3,350 and an array of neutralized and pH adjusted
organic acids, multivalent ions, a novel Citrate BIS-TRIS propane buffer system and pH (4 - 8.8). The
formulation of PEG/Ion 2 was developed at Hampton Research. Each of the 48 reagents in PEG/Ion 2
contains PEG 3,350 as the polymer (precipitant). The concentration of PEG is varied from 12% w/v to 20%
w/v depending upon the type and concentration of buffer/salt paired with the polymer. Thirteen of the
forty-eight PEG/Ion 2 reagents contain a separate buffer component. The remaining PEG/Ion 2 reagents
are buffered by the titrated organic acid salt. Six of these thirteen conditions feature a novel Citric acid BIS-
TRIS propane (CBTP) buffer. The CBTP buffer uses Citric acid and BIS-TRIS propane as the acid base pair
to create a two component buffer system effective across pH 2.5 to 9.5. The ratio of Citric acid to BIS-TRIS
propane determines the solution pH. Thirty-five of the forty-eight PEG/Ion 2 reagents contain a neutralized
or pH adjusted organic acid in the presence of the polymer. Neutralized organic acids are highly effective
crystallization salts.
1
Four PEG/Ion 2 reagents feature polyvalent cations. Two of these reagents contain
cation mixes, saving sample by screening six different cations with only two reagents. Tryptone, a casein
digest combinatorial library of peptides, is included in PEG/Ion 2.
PEG/Ion contains 48 unique reagents, 10 ml each.
PEG/Ion 2 contains 48 unique reagents, 10 ml each.
PEG/Ion HT contains 1 ml of each reagent from PEG/Ion and PEG/Ion 2 in a single Deep Well block
format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest
purity salts, polymers, organics and buffers.
PEG/Ion 2
Measured pH range of kit is 3.7 to 8.8 at 25C
Average measured pH of kit is 6.4 at 25C
Median measured pH of kit is 6.7 at 25C
Mode measured pH of kit is 6.7 at 25C
References
1. A comparison of salts for the crystallization of macromolecules. Alexander McPherson. Protein Science (2001), 10:418-422.
2. Searching for silver bullets: An alternative strategy for crystallizing macromolecules. Alexander McPherson and Bob Cudney. Journal of Structural Biology
Volume 156, Issue 3 , December 2006, Pages 387-406
d e s c r i p t i o n
Order Information
PEG/Ion Screen and PEG/Ion 2 Screen kits each contain 48 unique reagents. To order individual
reagents, use Custom Shop

catalog numbers listed below. Refer to page 36 for further details.


Cat. No. Name Description Price
HR2-126 PEG/Ion Screen 10 ml, tube format $285.00
HR2-098 PEG/Ion 2 Screen 10 ml, tube format $285.00
HR2-139 PEG/Ion HT 1 ml, Deep Well block format $185.00
HR2-922-** PEG/Ion Screen Custom Shop 185 ml $138.00
HR2-998-** PEG/Ion 2 Screen Custom Shop 185 ml $138.00
** = reagent number 1-48
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 12
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Crystals of Proteinase K. Preliminary crystallization con-
ditions obtained using PEG/Ion Screen from Hampton
Research.
Crystals grown at Hampton Research.
s u c c e s s s t o r y
P E G / I O N S C R E E N

P e G / I o n 2 s C r e e n

P e G / I o n H T

13
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s
A1
A2
A3
A4
A5
A6
A7
A8
A9
A10
A11
A12
B1
B2
B3
B4
B5
B6
B7
B8
B9
B10
B11
B12
C1
C2
C3
C4
C5
C6
C7
C8
C9
C10
C11
C12
D1
D2
D3
D4
D5
D6
D7
D8
D9
D10
D11
D12
E1
E2
E3
E4
E5
E6
E7
E8
E9
E10
E11
E12
F1
F2
F3
F4
F5
F6
F7
F8
F9
F10
F11
F12
G1
G2
G3
G4
G5
G6
G7
G8
G9
G10
G11
G12
H1
H2
H3
H4
H5
H6
H7
H8
H9
H10
H11
H12
P E G / I O N S C R E E N F O R M U L A T I O N
1. 0.2 M Sodium uoride, 20% w/v Polyethylene glycol 3,350
2. 0.2 M Potassium uoride, 20% w/v Polyethylene glycol 3,350
3. 0.2 M Ammonium uoride, 20% w/v Polyethylene glycol 3,350
4. 0.2 M Lithium chloride, 20% w/v Polyethylene glycol 3,350
5. 0.2 M Magnesium chloride hexahydrate, 20% w/v Polyethylene glycol 3,350
6. 0.2 M Sodium chloride, 20% w/v Polyethylene glycol 3,350
7. 0.2 M Calcium chloride dihydrate, 20% w/v Polyethylene glycol 3,350
8. 0.2 M Potassium chloride, 20% w/v Polyethylene glycol 3,350
9. 0.2 M Ammonium chloride, 20% w/v Polyethylene glycol 3,350
10. 0.2 M Sodium iodide, 20% w/v Polyethylene glycol 3,350
11. 0.2 M Potassium iodide, 20% w/v Polyethylene glycol 3,350
12. 0.2 M Ammonium iodide, 20% w/v Polyethylene glycol 3,350
13. 0.2 M Sodium thiocyanate, 20% w/v Polyethylene glycol 3,350
14. 0.2 M Potassium thiocyanate, 20% w/v Polyethylene glycol 3,350
15. 0.2 M Lithium nitrate, 20% w/v Polyethylene glycol 3,350
16. 0.2 M Magnesium nitrate hexahydrate, 20% w/v Polyethylene glycol 3,350
17. 0.2 M Sodium nitrate, 20% w/v Polyethylene glycol 3,350
18. 0.2 M Potassium nitrate, 20% w/v Polyethylene glycol 3,350
19. 0.2 M Ammonium nitrate, 20% w/v Polyethylene glycol 3,350
20. 0.2 M Magnesium formate dihydrate, 20% w/v Polyethylene glycol 3,350
21. 0.2 M Sodium formate, 20% w/v Polyethylene glycol 3,350
22. 0.2 M Potassium formate, 20% w/v Polyethylene glycol 3,350
23. 0.2 M Ammonium formate, 20% w/v Polyethylene glycol 3,350
24. 0.2 M Lithium acetate dihydrate, 20% w/v Polyethylene glycol 3,350
25. 0.2 M Magnesium acetate tetrahydrate, 20% w/v Polyethylene glycol 3,350
26. 0.2 M Zinc acetate dihydrate, 20% w/v Polyethylene glycol 3,350
27. 0.2 M Sodium acetate trihydrate, 20% w/v Polyethylene glycol 3,350
28. 0.2 M Calcium acetate hydrate, 20% w/v Polyethylene glycol 3,350
29. 0.2 M Potassium acetate, 20% w/v Polyethylene glycol 3,350
30. 0.2 M Ammonium acetate, 20% w/v Polyethylene glycol 3,350
31. 0.2 M Lithium sulfate monohydrate, 20% w/v Polyethylene glycol 3,350
32. 0.2 M Magnesium sulfate heptahydrate, 20% w/v Polyethylene glycol 3,350
33. 0.2 M Sodium sulfate decahydrate, 20% w/v Polyethylene glycol 3,350
34. 0.2 M Potassium sulfate, 20% w/v Polyethylene glycol 3,350
35. 0.2 M Ammonium sulfate, 20% w/v Polyethylene glycol 3,350
36. 0.2 M Sodium tartrate dibasic dihydrate, 20% w/v Polyethylene glycol 3,350
37. 0.2 M Potassium sodium tartrate tetrahydrate, 20% w/v Polyethylene glycol 3,350
38. 0.2 M Ammonium tartrate dibasic, 20% w/v Polyethylene glycol 3,350
39. 0.2 M Sodium phosphate monobasic monohydrate, 20% w/v Polyethylene glycol 3,350
40. 0.2 M Sodium phosphate dibasic dihydrate, 20% w/v Polyethylene glycol 3,350
41. 0.2 M Potassium phosphate monobasic, 20% w/v Polyethylene glycol 3,350
42. 0.2 M Potassium phosphate dibasic, 20% w/v Polyethylene glycol 3,350
43. 0.2 M Ammonium phosphate monobasic, 20% w/v Polyethylene glycol 3,350
44. 0.2 M Ammonium phosphate dibasic, 20% w/v Polyethylene glycol 3,350
45. 0.2 M Lithium citrate tribasic tetrahydrate, 20% w/v Polyethylene glycol 3,350
46. 0.2 M Sodium citrate tribasic dihydrate, 20% w/v Polyethylene glycol 3,350
47. 0.2 M Potassium citrate tribasic monohydrate, 20% w/v Polyethylene glycol 3,350
48. 0.2 M Ammonium citrate dibasic, 20% w/v Polyethylene glycol 3,350

1. 0.1 M Sodium malonate pH 4.0, 12% w/v Polyethylene glycol 3,350
2. 0.2 M Sodium malonate pH 4.0, 20% w/v Polyethylene glycol 3,350
3. 0.1 M Sodium malonate pH 5.0, 12% w/v Polyethylene glycol 3,350
4. 0.2 M Sodium malonate pH 5.0, 20% w/v Polyethylene glycol 3,350
5. 0.1 M Sodium malonate pH 6.0, 12% w/v Polyethylene glycol 3,350
6. 0.2 M Sodium malonate pH 6.0, 20% w/v Polyethylene glycol 3,350
7. 0.1 M Sodium malonate pH 7.0, 12% w/v Polyethylene glycol 3,350
8. 0.2 M Sodium malonate pH 7.0, 20% w/v Polyethylene glycol 3,350
9. 4% v/v Tacsimate pH 4.0, 12% w/v Polyethylene glycol 3,350
10. 8% v/v Tacsimate pH 4.0, 20% w/v Polyethylene glycol 3,350
11. 4% v/v Tacsimate pH 5.0, 12% w/v Polyethylene glycol 3,350
12. 8% v/v Tacsimate pH 5.0, 20% w/v Polyethylene glycol 3,350
13. 4% v/v Tacsimate pH 6.0, 12% w/v Polyethylene glycol 3,350
14. 8% v/v Tacsimate pH 6.0, 20% w/v Polyethylene glycol 3,350
15. 4% v/v Tacsimate pH 7.0, 12% w/v Polyethylene glycol 3,350
16. 8% v/v Tacsimate pH 7.0, 20% w/v Polyethylene glycol 3,350
17. 4% v/v Tacsimate pH 8.0, 12% w/v Polyethylene glycol 3,350
18. 8% v/v Tacsimate pH 8.0, 20% w/v Polyethylene glycol 3,350
19. 0.1 M Succinic acid pH 7.0, 12% w/v Polyethylene glycol 3,350
20. 0.2 M Succinic acid pH 7.0, 20% w/v Polyethylene glycol 3,350
21. 0.1 M Ammonium citrate tribasic pH 7.0, 12% w/v Polyethylene glycol 3,350
22. 0.2 M Ammonium citrate tribasic pH 7.0, 20% w/v Polyethylene glycol 3,350
23. 0.1 M DL-Malic acid pH 7.0, 12% w/v Polyethylene glycol 3,350
24. 0.2 M DL-Malic acid pH 7.0, 20% w/v Polyethylene glycol 3,350
25. 0.1 M Sodium acetate trihydrate pH 7.0, 12% w/v Polyethylene glycol 3,350
26. 0.2 M Sodium acetate trihydrate pH 7.0, 20% w/v Polyethylene glycol 3,350
27. 0.1 M Sodium formate pH 7.0, 12% w/v Polyethylene glycol 3,350
28. 0.2 M Sodium formate pH 7.0, 20% w/v Polyethylene glycol 3,350
29. 0.1 M Ammonium tartrate dibasic pH 7.0, 12% w/v Polyethylene glycol 3,350
30. 0.2 M Ammonium tartrate dibasic pH 7.0, 20% w/v Polyethylene glycol 3,350
31. 2% v/v Tacsimate pH 4.0, 0.1 M Sodium acetate trihydrate pH 4.6, 16% w/v Polyethylene glycol 3,350
32. 2% v/v Tacsimate pH 5.0, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 16% w/v Polyethylene glycol 3,350
33. 2% v/v Tacsimate pH 6.0, 0.1 M BIS-TRIS pH 6.5, 20% w/v Polyethylene glycol 3,350
34. 2% v/v Tacsimate pH 7.0, 0.1 M HEPES pH 7.5, 20% w/v Polyethylene glycol 3,350
35. 2% v/v Tacsimate pH 8.0, 0.1 M Tris pH 8.5, 16% w/v Polyethylene glycol 3,350
36. (0.07 M Citric acid, 0.03 M BIS-TRIS propane / pH 3.4), 16% w/v Polyethylene glycol 3,350
37. (0.06 M Citric acid, 0.04 M BIS-TRIS propane / pH 4.1), 16% w/v Polyethylene glycol 3,350
38. (0.05 M Citric acid, 0.05 M BIS-TRIS propane / pH 5.0), 16% w/v Polyethylene glycol 3,350
39. (0.04 M Citric acid, 0.06 M BIS-TRIS propane / pH 6.4), 20% w/v Polyethylene glycol 3,350
40. (0.03 M Citric acid, 0.07 M BIS-TRIS propane / pH 7.6), 20% w/v Polyethylene glycol 3,350
41. (0.02 M Citric acid, 0.08 M BIS-TRIS propane / pH 8.8), 16% w/v Polyethylene glycol 3,350
42. 0.02 M Calcium chloride dihydrate, 0.02 M Cadmium chloride hydrate,
0.02 M Cobalt(II) chloride hexahydrate, 20% w/v Polyethylene glycol 3,350
43. 0.01 M Magnesium chloride hexahydrate, 0.005 M Nickel(II) chloride hexahydrate
0.1 M HEPES sodium pH 7.0, 15% w/v Polyethylene glycol 3,350
44. 0.02 M Zinc chloride, 20% w/v Polyethylene glycol 3,350
45. 0.15 M Cesium chloride, 15% w/v Polyethylene glycol 3,350
46. 0.2 M Sodium bromide, 20% w/v Polyethylene glycol 3,350
47. 1% w/v Tryptone, 0.05 M HEPES sodium pH 7.0, 12% w/v Polyethylene glycol 3,350
48. 1% w/v Tryptone, 0.05 M HEPES sodium pH 7.0, 20% w/v Polyethylene glycol 3,350
P E G / I O N 2 S C R E E N F O R M U L A T I O N
m u l t i v a l e n t
i o n s
b u f f e r s
o r g a n i c s a l t s
Neutralized Organic Salts
BIS-TRIS
Citric acid BIS-TRIS propane (CBTP)
HEPES
HEPES sodium
Sodium acetate
Sodium citrate
Tris
Range from 4 to 8
p
H
Ammonium tartrate
Ammonium citrate
Malic acid
Succinic acid
Sodium acetate
Sodium formate
Sodium malonate
Tacsimate

Range from 4.0 to 9.0


Polyethylene glycol 3,350
Acetate
Citrate
Chloride
Fluoride
Formate
Iodide
Nitrate
Phosphate
Sulfate
Thiocyanate
Tartrate
p
H
a n i o n
p o l y m e r
Ammonium
Calcium
Lithium
Magnesium
Potassium
Sodium
Zinc
c a t i o n
Polyethylene glycol 3,350
p o l y m e r
Cadmium chloride
Calcium chloride
Cesium chloride
Cobalt(II) chloride
Magnesium chloride
Nickel(II) chloride
Sodium bromide
Zinc chloride
p e p t i d e s /
a m i n o a c i d s
Tryptone
p e g / i o n
f a c t o r s
p e g / i o n 2
f a c t o r s
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 14
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Grid Screen Salt HT combines Grid Screen
Ammonium Sulfate, Grid Screen Sodium
Malonate, Quik Screen and Grid Screen
Sodium Chloride into a single 96 Deep Well
block format. The block contains 1 ml of
each reagent.
Grid Screen Ammonium Sulfate systemati-
cally evaluates Ammonium sulfate at four
concentrations (0.8, 1.6, 2.4, 3.2 M) versus 6
pH levels (4, 5, 6, 7, 8, 9).
Grid Screen Sodium Malonate evaluates Sodium malonate at six concentrations (1.0, 1.5, 1.9, 2.4, 2.9,
3.4 M) versus four pH levels (4, 5, 6, 7).
Quik Screen evaluates Sodium potassium phosphate at four concentrations (0.8, 1.0, 1.4, 1.8 M) versus
six pH levels (5.0, 5.6, 6.3, 6.9, 7.5, 8.2).
Grid Screen Sodium Chloride evaluates Sodium chloride at four concentrations (1.0, 2.0, 3.0, 4.0 M)
versus six pH levels (4, 5, 6, 7, 8, 9).
Grid Screen PEG 6000 evaluates Polyethylene glycol at four concentrations (5, 10, 20, 30 %w/v) versus
six pH levels (4, 5, 6, 7, 8, 9).
Grid Screen MPD evaluates MPD at four concentrations (10, 20, 40, 65 %v/v) versus six pH levels (4,
5, 6, 7, 8, 9).
Grid Screen PEG/LiCl evaluates Polyethylene glycol at four concentrations (0, 10, 20, 30 %w/v) in the
presence of 1.0 M Lithium chloride versus six pH levels (4, 5, 6, 7, 8, 9).
Grid Screens and Quik Screen each contain 10 ml of 24 unique reagents.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the high-
est purity salts, polymers, organics and buffers.
References
1. A protein crystallization strategy using automated grid searches on successively finer grids. Patricia C. Weber. Methods: A Companion to Methods in
Enzymology Vol. 1, No. 1, August, pp. 31-37, 1990.
2. Protein Crystallization; Techniques, Strategies, and Tips. A Laboratory Manual. Edited by Terese M. Bergfors. International University Line, 1999. ISBN
0-9636817-5-3.
d e s c r i p t i o n
a p p l i c a t i o n
f e a t u r e s
n
Primary or secondary, salt, polymer, organic
and pH grid crystallization screen for biologi-
cal macromolecules
n
A systematic grid screen varying salt or poly-
mer or organic versus pH
n
Samples pH 4 to 9
n
Combine reagents within or between screens
to customize your screen
n
Combine reagents between rows or columns
to create expanded grid screens
n
Tube or Deep Well block format
Order Information
Each Grid Screen Salt HT kit contains 96 unique reagents.
Cat. No. Name Description Price
HR2-248 Grid Screen Salt HT 1 ml, Deep Well block format $185.00
G R I D S C R E E N S

q u I k s C r e e n


Number of crystals grown
versus varying A/S
concentrations [M]
A
m
m
oni um
S
ul f at e [ M
]
60
50
40
30
20
10
0
0
10
20
30
40
50
60
0
to
5
6
to
1
0
1
1
to
1
6
1
6
to
2
0
2
1
to
2
5
2
5
to
3
0
3
0
+
C
ry
s
ta
ls
G
ro
w
n
N
u
m
b
e
r

o
f

C
r
y
s
t
a
l
s
%
w
/v P
E
G
6000
Number of crystals grown
versus varying PEG 6000
concentrations (% w/v)
7
0

t
o

7
9
6
0

t
o

6
9
5
0

t
o

5
9
4
0

t
o

4
9
3
0

t
o

3
9
2
0

t
o

2
9
1
0

t
o

1
9
0

t
o

9
C
rystals G
ro
w
n
30
25
20
15
10
5
0
N
u
m
b
e
r

o
f

C
r
y
s
t
a
l
s
%
v/v M
PD
0
5
10
15
20
25
30
Number of crystals grown
versus varying MPD
concentrations (% v/v)
15
s
c
r
e
e
n
s
Cat. No. Name Description Price
HR2-211 Grid Screen Ammonium Sulfate 10 ml, tube format $175.00
HR2-924-** Grid Screen Ammonium Sulfate Custom Shop 185 ml $138.00
** = reagent number A1-D6
Order Information
Each Grid Screen and Quik Screen kit contains 24 unique reagents. To order individual reagents, use Custom Shop

catalog number
listed below. Refer to page 36 for further details.
Cat. No. Name Description Price
HR2-247 Grid Screen Sodium Malonate 10 ml, tube format $175.00
HR2-947-** Grid Screen Sodium Malonate Custom Shop 185 ml $138.00
** = reagent number A1-D6
Cat. No. Name Description Price
HR2-221 Quik Screen 10 ml, tube format $175.00
HR2-921-** Quik Screen Custom Shop 185 ml $138.00
** = reagent number A1-D6
Cat. No. Name Description Price
HR2-219 Grid Screen Sodium Chloride 10 ml, tube format $175.00
HR2-932-** Grid Screen Sodium Chloride Custom Shop 185 ml $138.00
** = reagent number A1-D6
Cat. No. Name Description Price
HR2-213 Grid Screen PEG 6000 10 ml, tube format $175.00
HR2-926-** Grid Screen PEG 6000 Custom Shop 185 ml $138.00
** = reagent number A1-D6
Cat. No. Name Description Price
HR2-215 Grid Screen MPD 10 ml, tube format $175.00
HR2-930-** Grid Screen MPD Custom Shop 185 ml $138.00
** = reagent number A1-D6
Cat. No. Name Description Price
HR2-217 Grid Screen PEG/LiCl 10 ml, tube format $175.00
HR2-928-** Grid Screen PEG/LiCl Custom Shop 185 ml $138.00
** = reagent number A1-D6
[ M ]
Sodium malonate
1
1.0
2
1.5
3
1.9
4
2.4
5
2.9
6
3.4
B
A
C
5.0
4.0
6.0
7.0 D
pH
[ M ]
Sodium/Potassium
Phosphate
1
5.0
2
5.6
3
6.3
4
6.9
5
7.5
6
8.2
B
A
C
1.0 M
0.8 M
1.4 M
1.8 M D
pH
0.1 M Buffer
[ M ]
Ammonium
sulfate
1
4
2
5
3
6
4
7
5
8
6
9
1.6 M B
0.8 M A
2.4 M C
3.0 M D
C
itr
ic
a
c
id
M
E
S
H
E
P
E
S
T
r
is
B
IC
IN
E
C
itr
ic
a
c
id
[ % w/v ]
Polyethylene
glycol
6,000
0.1 M Buffer
1
4
2
5
3
6
4
7
5
8
6
9
10% B
5% A
20% C
30% D
C
itr
ic
a
c
id
M
E
S
H
E
P
E
S
T
r
is
B
IC
IN
E
C
itr
ic
a
c
id
[ M ]
Sodium
chloride
0.1 M Buffer
1
4
2
5
3
6
4
7
5
8
6
9
2.0 M B
1.0 M A
3.0 M C
4.0 M D
C
itr
ic
a
c
id
M
E
S
H
E
P
E
S
T
r
is
B
IC
IN
E
C
itr
ic
a
c
id
[ % v/v ]
MPD
0.1 M Buffer
1
4
2
5
3
6
4
7
5
8
6
9
20% B
10% A
40% C
65% D
C
itr
ic
a
c
id
M
E
S
H
E
P
E
S
T
r
is
B
IC
IN
E
C
itr
ic
a
c
id
[ % w/v ]
Polyethylene
glycol
6,000
1.0 M Lithium chloride in all reagents
0.1 M Buffer
1
4
2
5
3
6
4
7
5
8
6
9
10% B
5% A
20% C
30% D
C
itr
ic
a
c
id
M
E
S
H
E
P
E
S
T
r
is
B
IC
IN
E
C
itr
ic
a
c
id
Crystals grown using SaltRx, reagent 62. They were
grown using the Cyberlab C240 robot and the Neuro
Probe plate. These were grown just in time to freeze
and take to the synchrotron (no time for optimizations).
MAD data were collected on these crystals and the
structure has been solved to 2.3 resolution.
Courtesy of Annie Hassell.
GlaxoSmithKline
s u c c e s s s t o r y
n
Primary or secondary, salt and pH matrix
crystallization screen for biological macromol-
ecules
n
Developed at Hampton Research
n
Salt versus pH matrix crystallization screen
n
Samples pH 4 - 9
n
22 unique salts versus salt concentration
and pH
n
Compatible with microbatch, vapor diffusion,
liquid & gel diffusion methods
n
Preformulated, ready to screen
n
All salts and buffers in screen readily avail-
able as Optimize reagents for reproducing
and optimizing crystals
n
Tube or Deep Well block format
a p p l i c a t i o n
f e a t u r e s
SaltRx was developed by Hampton Research
as a primary and secondary, salt and pH
based crystallization screen for biological
macromolecules. Salt is the only primary
crystallization reagent (precipitant) utilized.
Based on a design of 96 conditions, the
screen evaluates a broad portfolio of crystalli-
zation salts of varying concentration and pH.
The selection, concentration, and pH of the
salts were determined by data mining the BMCD
1
and other crystallization databases, crystallization reports
in the literature, and internal crystallization trials performed at Hampton Research. Based on this analysis,
up to 35% of protein crystallizations involve salt as the primary crystallization reagent.
SaltRx can be used as a primary crystallization screen when salt, ionic strength and pH are desired or
suspected as appropriate crystallization variables. It is also useful as a secondary screen when salt based
reagents/conditions from screens such as Index, Crystal Screen, and Grid Screen produce crystals and
when further screening for additional salt conditions or optimization is desired.
SaltRx is designed to be used as a 96 reagent screen.
SaltRx 1 contains 48 unique reagents, 10 ml each.
SaltRx 2 contains 48 unique reagents, 10 ml each.
SaltRx HT contains 1 ml of each reagent from SaltRx 1 and SaltRx 2 in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest
purity salts, polymers, organics and buffers.
References
1. Gilliland, G.L., Tung, M., Blakeslee, D.M. and Ladner, J. 1994. The Biological Macromolecule Crystallization Database, Version 3.0: New
Features, Data, and the NASAArchive for Protein Crystal Growth Data. Acta Crystallogr. D50 408-413.
2. Crystallization and preliminary crystallographic analysis of molybdenumcofactor biosynthesis protein C from Thermus thermophilus. S. P. Kanaujia, C. V.
Ranjani, J. Jeyakanthan, S. Baba, L. Chen, Z.-J. Liu, B.-C. Wang, M. Nishida, A. Ebihara, A. Shinkai, S. Kuramitsu, Y. Shiro,
K. Sekar and S. Yokoyama. Acta Cryst. (2007). F63, 27-29.
Order Information
SaltRx 1 and SaltRx 2 kits each contain 48 unique reagents. To order individual reagents, use
Custom Shop

catalog number listed below. Refer to page 36 for further details.


Cat. No. Name Description Price
HR2-107 SaltRx 1 10 ml, tube format $285.00
HR2-109 SaltRx 2 10 ml, tube format $285.00
HR2-136 SaltRx HT 1 ml, Deep Well block format $185.00
HR2-907-** SaltRx 1 Custom Shop 185 ml $138.00
HR2-909-** SaltRx 2 Custom Shop 185 ml $138.00
** = reagent number 1-48
d e s c r i p t i o n
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 16
s
c
r
e
e
n
s
S A L T R X 1

s a l T r x 2

s a l T r x H T

17
s
c
r
e
e
n
s
A1
A2
A3
A4
A5
A6
A7
A8
A9
A10
A11
A12
B1
B2
B3
B4
B5
B6
B7
B8
B9
B10
B11
B12
C1
C2
C3
C4
C5
C6
C7
C8
C9
C10
C11
C12
D1
D2
D3
D4
D5
D6
D7
D8
D9
D10
D11
D12
E1
E2
E3
E4
E5
E6
E7
E8
E9
E10
E11
E12
F1
F2
F3
F4
F5
F6
F7
F8
F9
F10
F11
F12
G1
G2
G3
G4
G5
G6
G7
G8
G9
G10
G11
G12
H1
H2
H3
H4
H5
H6
H7
H8
H9
H10
H11
H12
S A L T R X 1 F O R M U L A T I O N
1. 1.8 M Sodium acetate trihydrate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0
2. 2.8 M Sodium acetate trihydrate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0
3. 1.5 M Ammonium chloride, 0.1 M Sodium acetate trihydrate pH 4.6
4. 1.5 M Ammonium chloride, 0.1 M BIS-TRIS propane pH 7.0
5. 1.5 M Ammonium chloride, 0.1 M Tris pH 8.5
6. 3.5 M Ammonium chloride, 0.1 M Sodium acetate trihydrate pH 4.6
7. 3.5 M Ammonium chloride, 0.1 M BIS-TRIS propane pH 7.0
8. 3.5 M Ammonium chloride, 0.1 M Tris pH 8.5
9. 2.2 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 4.6
10. 2.2 M Sodium chloride, 0.1 M BIS-TRIS propane pH 7.0
11. 2.2 M Sodium chloride, 0.1 M Tris pH 8.5
12. 3.2 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 4.6
13. 3.2 M Sodium chloride, 0.1 M BIS-TRIS propane pH 7.0
14. 3.2 M Sodium chloride, 0.1 M Tris pH 8.5
15. 1.0 M Ammonium citrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6
16. 1.8 M Ammonium citrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6
17. 1.0 M Ammonium citrate tribasic pH 7.0, 0.1 M BIS-TRIS propane pH 7.0
18. 2.0 M Ammonium citrate tribasic pH 7.0, 0.1 M BIS-TRIS propane pH 7.0
19. 0.7 M Sodium citrate tribasic dihydrate, 0.1 M BIS-TRIS propane pH 7.0
20. 0.7 M Sodium citrate tribasic dihydrate, 0.1 M Tris pH 8.5
21. 1.2 M Sodium citrate tribasic dihydrate, 0.1 M BIS-TRIS propane pH 7.0
22. 1.2 M Sodium citrate tribasic dihydrate, 0.1 M Tris pH 8.5
23. 0.4 M Magnesium formate dihydrate, 0.1 M Sodium acetate trihydrate pH 4.6
24. 0.4 M Magnesium formate dihydrate, 0.1 M BIS-TRIS propane pH 7.0
25. 0.4 M Magnesium formate dihydrate, 0.1 M Tris pH 8.5
26. 0.7 M Magnesium formate dihydrate, 0.1 M BIS-TRIS propane pH 7.0
27. 2.0 M Sodium formate, 0.1 M Sodium acetate trihydrate pH 4.6
28. 2.0 M Sodium formate, 0.1 M BIS-TRIS propane pH 7.0
29. 2.0 M Sodium formate, 0.1 M Tris pH 8.5
30. 3.5 M Sodium formate, 0.1 M Sodium acetate trihydrate pH 4.6
31. 3.5 M Sodium formate, 0.1 M BIS-TRIS propane pH 7.0
32. 3.5 M Sodium formate, 0.1 M Tris pH 8.5
33. 1.2 M DL-Malic acid pH 7.0, 0.1 M BIS-TRIS propane pH 7.0
34. 2.2 M DL-Malic acid pH 7.0, 0.1 M BIS-TRIS propane pH 7.0
35. 1.4 M Sodium malonate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0
36. 2.4 M Sodium malonate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0
37. 2.5 M Ammonium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6
38. 2.5 M Ammonium nitrate, 0.1 M BIS-TRIS propane pH 7.0
39. 2.5 M Ammonium nitrate, 0.1 M Tris pH 8.5
40. 6.0 M Ammonium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6
41. 6.0 M Ammonium nitrate, 0.1 M BIS-TRIS propane pH 7.0
42. 6.0 M Ammonium nitrate, 0.1 M Tris pH 8.5
43. 1.5 M Sodium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6
44. 1.5 M Sodium nitrate, 0.1 M BIS-TRIS propane pH 7.0
45. 1.5 M Sodium nitrate, 0.1 M Tris pH 8.5
46. 4.0 M Sodium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6
47. 4.0 M Sodium nitrate, 0.1 M BIS-TRIS propane pH 7.0
48. 4.0 M Sodium nitrate, 0.1 M Tris pH 8.5
1. 1.0 M Ammonium phosphate monobasic, 0.1 M Sodium acetate trihydrate pH 4.6
2. 1.8 M Ammonium phosphate monobasic, 0.1 M Sodium acetate trihydrate pH 4.6
3. 1.5 M Ammonium phosphate dibasic, 0.1 M Tris pH 8.5
4. 2.4 M Ammonium phosphate dibasic, 0.1 M Tris pH 8.5
5. 1.0 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 5.0
6. 1.0 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 6.9
7. 1.0 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 8.2
8. 1.8 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 5.0
9. 1.8 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 6.9
10. 1.8 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 8.2
11. 0.5 M Succinic acid pH 7.0, 0.1 M BIS-TRIS propane pH 7.0
12. 1.0 M Succinic acid pH 7.0, 0.1 M BIS-TRIS propane pH 7.0
13. 1.5 M Ammonium sulfate, 0.1 M Sodium acetate trihydrate pH 4.6
14. 1.5 M Ammonium sulfate, 0.1 M BIS-TRIS propane pH 7.0
15. 1.5 M Ammonium sulfate, 0.1 M Tris pH 8.5
16. 2.5 M Ammonium sulfate, 0.1 M Sodium acetate trihydrate pH 4.6
17. 2.5 M Ammonium sulfate, 0.1 M BIS-TRIS propane pH 7.0
18. 2.5 M Ammonium sulfate, 0.1 M Tris pH 8.5
19. 0.8 M Lithium sulfate monohydrate, 0.1 M Sodium acetate trihydrate pH 4.6
20. 0.8 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS propane pH 7.0
21. 0.8 M Lithium sulfate monohydrate, 0.1 M Tris pH 8.5e
22. 1.5 M Lithium sulfate monohydrate, 0.1 M Sodium acetate trihydrate pH 4.6
23. 1.5 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS propane pH 7.0
24. 1.5 M Lithium sulfate monohydrate, 0.1 M Tris pH 8.5
25. 1.0 M Magnesium sulfate hydrate, 0.1 M Sodium acetate trihydrate pH 4.6
26. 1.0 M Magnesium sulfate hydrate, 0.1 M BIS-TRIS propane pH 7.0
27. 1.0 M Magnesium sulfate hydrate, 0.1 M Tris pH 8.5
28. 1.8 M Magnesium sulfate hydrate, 0.1 M Sodium acetate trihydrate pH 4.6
29. 1.8 M Magnesium sulfate hydrate, 0.1 M BIS-TRIS propane pH 7.0
30. 1.8 M Magnesium sulfate hydrate, 0.1 M Tris pH 8.5
31. 0.7 M Ammonium tartrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6
32. 0.7 M Ammonium tartrate dibasic, 0.1 M BIS-TRIS propane pH 7.0
33. 0.7 M Ammonium tartrate dibasic, 0.1 M Tris pH 8.5
34. 1.1 M Ammonium tartrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6
35. 1.3 M Ammonium tartrate dibasic, 0.1 M BIS-TRIS propane pH 7.0
36. 1.4 M Ammonium tartrate dibasic, 0.1 M Tris pH 8.5
37. 0.6 M Potassium sodium tartrate tetrahydrate 0.1 M BIS-TRIS propane pH 7.0
38. 1.2 M Potassium sodium tartrate tetrahydrate 0.1 M BIS-TRIS propane pH 7.0
39. 0.6 M Potassium sodium tartrate tetrahydrate, 0.1 M Tris pH 8.5
40. 1.2 M Potassium sodium tartrate tetrahydrate, 0.1 M Tris pH 8.5
41. 0.5 M Potassium thiocyanate, 0.1 M Sodium acetate trihydrate pH 4.6
42. 0.5 M Potassium thiocyanate, 0.1 M BIS-TRIS propane pH 7.0
43. 0.5 M Potassium thiocyanate, 0.1 M Tris pH 8.5
44. 4.0 M Ammonium acetate, 0.1 M Sodium acetate trihydrate pH 4.6
45. 0.1 M BIS-TRIS propane pH 7.0, 4.0 M Ammonium Acetate
46. 4.0 M Ammonium acetate, 0.1 M Tris pH 8.5
47. 35% v/v Tacsimate

, 0.1 M BIS-TRIS propane pH 7.0


48. 60% v/v Tacsimate

, 0.1 M BIS-TRIS propane pH 7.0


S A L T R X 2 F O R M U L A T I O N
Ammonium citrate dibasic
Ammonium citrate tribasic
Magnesium formate dihydrate
Sodium acetate
Sodium citrate tribasic dihydrate
Sodium formate
Sodium malonate
DL-Malic acid
Range from 4.6 to 8.5
p H
o r g a n i c s a l t
Ammonium chloride
Sodium chloride
Ammonium nitrate
Sodium nitrate
s a l t
BIS-TRIS propane
Sodium acetate trihydrate
Tris
Ammonium acetate
Ammonium tartrate dibasic
K/Na Tartrate
Succinic acid
Tacsimate
TM
Range from 4.6 to 8.5
buffer
p H
o r g a n i c s a l t
Ammonium phosphate mono.
Ammonium phosphate dibasic
Ammonium sulfate
Lithium sulfate monohydrate
Magnesium sulfate hydrate
Na/K Phosphate
Potassium thiocyanate
s a l t
BIS-TRIS propane
Sodium acetate trihydrate
Tris
buffer
s a l t r x 1
f a c t o r s
s a l t r x 2
f a c t o r s
Crystals above are those of the membrane protein
succinate:quinone oxireductase. Preliminary crystalliza-
tion conditions determined using MembFac.
Courtesy of Michael Stowell.
MRC-LMB
s u c c e s s s t o r y
n
Primary sparse matrix crystallization screen
for membrane proteins and samples with
limited solubility
n
Membrane protein sparse matrix screens
1
n
Sparse matrix formula efficiently samples
salts, polymers, organics and pH
n
pH range 4.6 - 8.5
n
Formulated for use with detergents
n
Crystal Screen Lite features a lower ionic
strength than the original Crystal Screen
n
Tube or Deep Well block format
a p p l i c a t i o n
f e a t u r e s
MembFac is a highly effective sparse matrix
screen specifically designed as a preliminary
screen for the crystallization ofmembrane pro-
teins. MembFac is based upon the highly effec-
tive biased sparse matrix methodology. The
MembFac screen matrix is optimized for the
crystallization of membrane proteins.
MembFac contains 48 unique reagent formula-
tions. With this set of 48 conditions, numer-
ous chemicals and chemical combinations are
utilized, allowing one to evaluate a large variety of potential crystallization conditions. MembFac covers a
broad pH range between 4 and 9.
The Rationale
The basic rationale of MembFac is to perform a sparse matrix screen while changing the detergent dimen-
sion because detergents play an important factor in the crystallization of membrane proteins. A MembFac
screen is to be completed for each detergent. Detergent screens are available separately.
The Method
The membrane protein of interest is first isolated in the detergent which gives the highest stability and
activity. The protein concentration should be approximately 10 to 20 mg/ml and the detergent concentra-
tion should be only slightly above the CMC. The sample is then set against MembFac, using all 48 solutions
plus the screening detergent(s) of choice. The screening detergent concentration should be approximately
one to three times the CMC. Hence, for each detergent screened, one would utilize approximately 1 to 2
mg of sample.
The MembFac protocol was used to crystallize membrane proteins including SQR, fumerate: quinone
oxireductase, LHII, and LHCII.
Crystal Screen Lite is a sparse matrix of trial crystallization reagent conditions based upon the Crystal
Screen.
3
The primary screen variables are salt, pH, and precipitant (salts, polymers, volatile organics, and
non-volatile organics).
2
Crystal Screen Lite differs from the original Crystal Screen kit in that the primary
precipitant reagents are one-half the concentration of that used in the original Crystal Screen formulation.
The secondary salts, ions, and buffers remain at the original Crystal Screen concentration. Reducing the
primary concentration of the primary precipitant results in a screen which is more gentle on the sample
and typically produces much less precipitate conditions than the original Crystal Screen.
MembFac contains 48 unique reagents, 10 ml each.
MembFac HT contains 1 ml each of all 48 reagents from MembFac and reagents 1-48 from Crystal Screen
Lite in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest
purity salts, polymers, organics and buffers.
References
1. UCLA Crystallization Workshop, June 21, 1993.
2. Crystallization of nucleic acids and proteins, Edited by A. Ducruix and R. Giege, The Practical Approach Series, Oxford Univ. Press, 1992.
3. McPherson, A., Current approaches to macromolecular crystallization., Eur. J. Biochem. (1990) 189, 1-23.
4. Jancarik, J. and Kim, S.H., Sparse Matrix Sampling: a screening method for crystallization of proteins. (1991) J. Appl. Cryst., 24,409-411.
5. Protein and Nucleic Acid Crystallization. Methods, A Companion to Methods in Enzymology, Academic Press, Volume 1, Number 1, August 1990.
Order Information
Each MembFac kit contains 48 unique reagents. Crystal Screen Lite kit contains 50 unique
reagents. To order individual reagents, use Custom Shop

catalog number listed below. Refer to


page 36 for further details.
Cat. No. Name Description Price
HR2-114 MembFac 10 ml, tube format $285.00
HR2-128 Crystal Screen Lite 10 ml, tube format $285.00
HR2-137 MembFac HT 1 ml, Deep Well block format $185.00
HR2-920-** MembFac Custom Shop 185 ml $138.00
HR2-916-** Crystal Screen Lite Custom Shop 185 ml $138.00
** = reagent number 1-48 (for MembFac)
** = reagent number 1-50 (for Crystal Screen Lite)
d e s c r i p t i o n
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 18
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M E M B F A C

C r y s T a l s C r e e n l I T e

M e M b F a C H T

19
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Range from 4.6 to 8.5
2-Propanol
(+/-)-2-Methyl-2,4-pentanediol
Polyethylene glycol 400
Polyethylene glycol 4,000
Polyethylene glycol 6,000
Magnesium chloride
Sodium chloride
Ammonium sulfate
Lithium sulfate
Magnesium sulfate
Ammonium phosphate
Potassium phosphate
Sodium phosphate
Sodium citrate
K/Na tartrate
o r g a n i c
p
H
s a l t
p o l y m e r
n o n - v o l a t i l e
o r g a n i c
A1
A2
A3
A4
A5
A6
A7
A8
A9
A10
A11
A12
B1
B2
B3
B4
B5
B6
B7
B8
B9
B10
B11
B12
C1
C2
C3
C4
C5
C6
C7
C8
C9
C10
C11
C12
D1
D2
D3
D4
D5
D6
D7
D8
D9
D10
D11
D12
E1
E2
E3
E4
E5
E6
E7
E8
E9
E10
E11
E12
F1
F2
F3
F4
F5
F6
F7
F8
F9
F10
F11
F12
G1
G2
G3
G4
G5
G6
G7
G8
G9
G10
G11
G12
H1
H2
H3
H4
H5
H6
H7
H8
H9
H10
H11
H12
1. 0.1 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 4.6, 12% v/v (+
/
-)-2-Methyl-2,4-pentanediol
2. 0.1 M Zinc acetate dihydrate, 0.1 M Sodium acetate trihydrate pH 4.6, 12% w/v Polyethylene glycol 4,000
3. 0.2 M Ammonium sulfate, 0.1 M Sodium acetate trihydrate pH 4.6, 10% w/v Polyethylene glycol 4,000
4. 0.1 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 4.6, 12% v/v 2-Propanol
5. 0.1 M Sodium acetate trihydrate pH 4.6, 12% w/v Polyethylene glycol 4,000
6. 0.1 M Sodium acetate trihydrate pH 4.6, 1.0 M Ammonium sulfate
7. 0.1 M Sodium acetate trihydrate pH 4.6, 1.0 M Magnesium sulfate heptahydrate
8. 0.1 M Magnesium chloride hexahydrate, 0.1 M Sodium acetate trihydrate pH 4.6, 18% v/v Polyethylene glycol 400
9. 0.1 M Lithium sulfate monohydrate, 0.1 M Sodium acetate trihydrate pH 4.6, 1.0 M Ammonium phosphate monobasic
10. 0.1 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 4.6, 12% w/v Polyethylene glycol 6,000
11. 0.1 M Magnesium chloride hexahydrate, 0.1 M Sodium acetate trihydrate pH 4.6, 12% w/v Polyethylene glycol 6,000
12. 0.1 M Sodium chloride, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 18% v/v Polyethylene glycol 400
13. 0.1 M Lithium sulfate monohydrate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 12 % w/v Polyethylene glycol 4,000
14. 0.1 M Sodium citrate tribasic dihydrate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 10% v/v 2-Propanol
15. 0.1 M Sodium chloride, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 12% v/v (+
/
-)-2-Methyl-2,4-pentanediol
16. 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 1.0 M Magnesium sulfate heptahydrate
17. 0.1 M Sodium chloride, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 12% w/v Polyethylene glycol 4,000
18. 0.1 M Lithium sulfate monohydrate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 12% w/v Polyethylene glycol 6,000
19. 0.1 M Magnesium chloride hexahydrate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 4% v/v (+
/
-)-2-Methyl-2,4-pentanediol
20. 0.1 M Sodium citrate trihydrate dihydrate pH 5.6, 0.1 M Sodium chloride
21. 0.1 M Lithium sulfate monohydrate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 4% v/v Polyethylene glycol 400
22. 0.1 M ADA pH 6.5, 1.0 M Ammonium sulfate
23. 0.1 M Lithium sulfate monohydrate, 0.1 M ADA pH 6.5, 12% w/v Polyethylene glycol 4,000, 2% v/v 2-Propanol
24. 0.1 M ADA pH 6.5, 1.0 M Ammonium phosphate dibasic
25. 0.1 M Magnesium chloride hexahydrate, 0.1 M ADA pH 6.5, 12% w/v Polyethylene glycol 6,000
26. 0.1 M ADA pH 6.5, 12% v/v (+
/
-)-2-Methyl-2,4-pentanediol
27. 0.1 M Lithium sulfate monohydrate, 0.1 M ADA pH 6.5, 1.0 M Magnesium sulfate hydrate
28. 0.3 M Lithium sulfate monohydrate, 0.1 M ADA pH 6.5, 4% v/v Polyethylene glycol 400
29. 0.1 M Ammonium sulfate, 0.1 M HEPES sodium pH 7.5, 0.5 M Sodium phosphate dibasic dihydrate,
0.5 M Potassium phosphate dibasic
30. 0.1 M Sodium chloride, 0.1 M HEPES sodium pH 7.5, 10% w/v Polyethylene glycol 4,000
31. 0.1 M Magnesium chloride hexahydrate, 0.1 M HEPES sodium pH 7.5, 18% v/v Polyethylene glycol 400
32. 0.1 M HEPES sodium pH 7.5, 1.0 M Potassium sodium tartrate tetrahydrate
33. 0.1 M Ammonium sulfate, 0.1 M HEPES sodium pH 7.5, 18% v/v Polyethylene glycol 400
34. 0.1 M Ammonium sulfate, 0.1 M HEPES sodium pH 7.5, 10% w/v Polyethylene glycol 4,000
35. 0.1 M Sodium citrate tribasic dihydrate, 0.1 M HEPES sodium pH 7.5, 12% v/v (+
/
-)-2-Methyl-2,4-pentanediol
36. 0.1 M HEPES sodium pH 7.5, 1.0 M Sodium citrate tribasic dihydrate
37. 0.6 M Magnesium sulfate hydrate, 0.1 M HEPES sodium pH 7.5, 4% v/v Polyethylene glycol 400
38. 0.6 M Magnesium sulfate hydrate, 0.1 M HEPES sodium pH 7.5, 4% v/v (+
/
-)-2-Methyl-2,4-pentanediol
39. 0.1 M Lithium sulfate monohydrate, 0.1 M HEPES sodium pH 7.5, 0.1 M Potassium sodium tartrate tetrahydrate
40. 0.1 M Lithium sulfate monohydrate, 0.1 M TRIS hydrochloride pH 8.5, 12% v/v (+
/
-)-2-Methyl-2,4-pentanediol
41. 0.1 M Ammonium phosphate dibasic, 0.1 M TRIS hydrochloride pH 8.5, 0.5 M Sodium phosphate dibasic dihydrate,
0.5 M Potassium phosphate dibasic
42. 0.1 M TRIS hydrochloride pH 8.5, 0.1 M Sodium acetate trihydrate
43. 0.1 M TRIS hydrochloride pH 8.5, 0.1 M Sodium chloride
44. 0.1 M Ammonium phosphate dibasic, 0.1 M TRIS hydrochloride pH 8.5, 12% w/v Polyethylene glycol 6,000
45. 0.1 M Potassium sodium tartrate tetrahydrate, 0.1 M TRIS hydrochloride pH 8.5, 0.4 M Magnesium sulfate hydrate
46. 0.1 M TRIS hydrochloride pH 8.5, 0.2 M Lithium sulfate monohydrate
47. 0.1 M TRIS hydrochloride pH 8.5, 0.5 M Ammonium sulfate
48. 0.1 M Sodium citrate tribasic dihydrate, 0.1 M TRIS hydrochloride pH 8.5, 5% v/v Polyethylene glycol 400
1. 0.02 M Calcium chloride dihydrate, 0.1 M Sodium acetate trihydrate pH 4.6, 15% v/v (+
/
-)-2-Methyl-2,4-pentanediol
2. 0.2 M Potassium sodium tartrate tetrahydrate
3. 0.2 M Ammonium phosphate monobasic
4. 0.1 M TRIS hydrochloride pH 8.5, 1.0 M Ammonium sulfate
5. 0.2 M Sodium citrate tribasic dihydrate, 0.1 M HEPES sodium pH 7.5, 15% v/v (+
/
-)-2-Methyl-2,4-pentanediol
6. 0.2 M Magnesium chloride hexahydrate, 0.1 M TRIS hydrochloride pH 8.5, 15% w/v Polyethylene glycol 4,000
7. 0.1 M Sodium cacodylate trihydrate pH 6.5, 0.7 M Sodium acetate trihydrate
8. 0.2 M Sodium citrate tribasic dihydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 15% v/v 2-Propanol
9. 0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 15% w/v Polyethylene glycol 4,000
10. 0.2 M Ammonium acetate, 0.1 M Sodium acetate trihydrate pH 4.6, 15% w/v Polyethylene glycol 4,000
11. 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 0.5 M Ammonium phosphate monobasic
12. 0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES sodium pH 7.5, 15% v/v 2-Propanol
13. 0.2 M Sodium citrate tribasic dihydrate, 0.1 M TRIS hydrochloride pH 8.5, 15% v/v Polyethylene glycol 400
14. 0.2 M Calcium chloride dihydrate, 0.1 M HEPES sodium pH 7.5, 14% v/v Polyethylene glycol 400
15. 0.2 M Ammonium sulfate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 15% w/v Polyethylene glycol 8,000
16. 0.1 M HEPES sodium pH 7.5, 0.75 M Lithium sulfate monohydrate
17. 0.2 M Lithium sulfate monohydrate, 0.1 M TRIS hydrochloride pH 8.5, 15% w/v Polyethylene glycol 4,000
18. 0.2 M Magnesium acetate tetrahydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 10% w/v Polyethylene glycol 8,000
19. 0.2 M Ammonium acetate, 0.1 M TRIS hydrochloride pH 8.5, 15% v/v 2-Propanol
20. 0.2 M Ammonium sulfate, 0.1 M Sodium acetate trihydrate pH 4.6, 12.5% w/v Polyethylene glycol 4,000
21. 0.2 M Magnesium acetate tetrahydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 15% v/v (+
/
-)-2-Methyl-2,4-pentanediol
22. 0.2 M Sodium acetate trihydrate, 0.1 M TRIS hydrochloride pH 8.5, 15% w/v Polyethylene glycol 4,000
23. 0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES sodium pH 7.5, 15% v/v Polyethylene glycol 400
24. 0.2 M Calcium chloride dihydrate, 0.1 M Sodium acetate trihydrate pH 4.6, 10% v/v 2-Propanol
25. 0.1 M Imidazole pH 6.5, 0.5 M Sodium acetate trihydrate
26. 0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 15% v/v (+
/
-)-2-Methyl-2,4-pentanediol
27. 0.2 M Sodium citrate tribasic dihydrate, 0.1 M HEPES sodium pH 7.5, 10% v/v 2-Propanol
28. 0.2 M Sodium acetate trihydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 15% w/v Polyethylene glycol 8,000
29. 0.1 M HEPES sodium pH 7.5, 0.4 M Potassium sodium tartrate tetrahydrate
30. 0.2 M Ammonium sulfate, 15% w/v Polyethylene glycol 8,000
31. 0.2 M Ammonium sulfate, 15% w/v Polyethylene glycol 4,000
32. 1.0 M Ammonium sulfate
33. 2.0 M Sodium formate
34. 0.1 M Sodium acetate trihydrate pH 4.6, 1.0 M Sodium formate
35. 0.1 M HEPES sodium pH 7.5, 0.4 M Sodium phosphate monobasic monohydrate, 0.4 M Potassium phosphate monobasic
36. 0.1 M TRIS hydrochloride pH 8.5, 4% w/v Polyethylene glycol 8,000
37. 0.1 M Sodium acetate trihydrate pH 4.6, 4% w/v Polyethylene glycol 4,000
38. 0.1 M HEPES sodium pH 7.5, 0.7 M Sodium citrate tribasic dihydrate
39. 0.1 M HEPES sodium pH 7.5, 2% v/v Polyethylene glycol 400, 1.0 M Ammonium sulfate
40. 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 10% v/v 2-Propanol, 10% w/v Polyethylene glycol 4,000
41. 0.1 M HEPES sodium pH 7.5, 5% v/v 2-Propanol, 10% w/v Polyethylene glycol 4,000
42. 0.05 M Potassium phosphate monobasic, 10% w/v Polyethylene glycol 8,000
43. 15% w/v Polyethylene glycol 1,500
44. 0.1 M Magnesium formate dihydrate
45. 0.2 M Zinc acetate dihydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 9% w/v Polyethylene glycol 8,000
46. 0.2 M Calcium acetate hydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 9% w/v Polyethylene glycol 8,000
47. 0.1 M Sodium acetate trihydrate pH 4.6, 1.0 M Ammonium sulfate
48. 0.1 M TRIS hydrochloride pH 8.5, 1.0 M Ammonium phosphate monobasic
49. 0.5 M Lithium sulfate monohydrate, 2% w/v Polyethylene glycol 8,000
50.

0.5 M Lithium sulfate monohydrate, 7.5% w/v Polyethylene glycol 8,000
C R Y S T A L S C R E E N L I T E F O R M U L A T I O N
M E M B F A C F O R M U L A T I O N
Range from 4.0 to 9.0
2-Propanol
(+/-)-2-Methyl-2,4-pentanediol
Polyethylene glycol 400
Polyethylene glycol 1,500
Polyethylene glycol 4,000
Polyethylene glycol 8,000
Ammonium acetate
Calcium acetate
Magnesium acetate
Sodium acetate
Zinc acetate
Calcium chloride
Magnesium chloride
Sodium citrate
Magnesium formate
Sodium formate
Ammonium phosphate
Potassium phosphate
Sodium phosphate
Ammonium sulfate
Lithium sulfate
K/Na tartrate
o r g a n i c
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s a l t
p o l y m e r
HEPES sodium
Imidazole
Sodium acetate
Sodium cacodylate
Sodium citrate
TRIS hydrochloride
b u f f e r
m e m b f a c
f a c t o r s
crystal screen lite
f a c t o r s
n
Crystallization screen for nucleic acids &
protein/nucleic acid complexes
n
Nucleic acid sparse matrix screen
1
n
Sparse matrix formula efficiently samples
salts, polyols, organics, & pH
n
pH range 5.6 - 8.5
n
Screen Magnesium, Potassium,
& Ammonium anions
a p p l i c a t i o n
f e a t u r e s
Natrix is based upon a published protocol
which is highly effective for the crystallization
of nucleic acids and protein-nucleic acid com-
plexes. A variety of hammerhead ribozymes
and other ribozymes, RNAs, DNAs, RNA-drug
complexes, and RNA-protein complexes have
been crystallized using the Natrix protocol.
By using sparse matrix sampling technology,
Natrix allows one to quickly test wide ranges
of pH, salts, and precipitants using a very small sample (50 to 100 l) of nucleic acid.
Natrix is unique in that rather than relying solely on the traditional nucleic acid precipitant MPD, Natrix
also utilizes Polyethylene glycols (PEGs) in a variety of molecular weights (200, 400, 4,000, 8,000) as well as
2-Propanol, Polyethylene glycol monomethyl ether (PEG MME), and 1,6-Hexanediol. Many of the polymeric
and low molecular weight organic precipitants are combined with various monovalent salts as precipitating
agents. This combination of salts and low molecular weight organics and polyalcohols, as well as the utiliza-
tion of varying chain length PEGs, has proven to be a successful combination for producing nucleic acid
and protein-nucleic acid complex crystals.
RNA Crystallization
Typical crystallization trials using Natrix will use the hanging or sitting drop methodology. Sample prepara-
tion is straightforward. The highly purified sample is heated to 75C and annealed in the presence of the
appropriate polyamine and buffer. After cooling to room temperature, the sample is mixed with an equal
amount of Natrix solution and set against a reservoir containing the same Natrix solution for vapor diffusion
equilibration. Typically, crystals are observed within the first week of setting up the trial.
Each Natrix contains 48 unique reagents of varying salt and precipitant composition over six different
pH levels. Each preformulated, sterile filtered solution provides sufficient reagent for more than a dozen
screens. The method is suitable for hanging drop, sitting drop, sandwich drop, and microbatch crystalliza-
tion methodologies. Crystallization plates are sold separately. Natrix does not include polyamines. It is rec-
ommended that a polyamine such as Spermine be added to the sample prior to screening and that various
polyamines be screened AFTER preliminary crystallization conditions have been determined.
Each Natrix kit contains 48 unique reagents, 10 ml each. Ready-to-use reagents are sterile filtered and
formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics, and buffers.
References
1. Scott, W. G., Finch, J.T., Grenfell, R., Fogg, J., Smith, T., Gait, M.J., Klug, A., Journal of Molecular Biology (1995) 250: 327-332.
2. Purification, crystallization and preliminary X-ray analysis of the BseCI DNA methyltransferase from Bacillus stearothermophilus in complex with it's cognate
DNA. E. G. Kapetaniou, D. Kotsifaki, M. Providaki, M. Rina, V. Bouriotis and M. Kokkinidis. Acta Cryst. (2007). F63, 12-14.
3. Crystallization and preliminary X-ray diffraction analysis of an Escherichia coli tRNAGly acceptor-stem microhelix. C. Frster, M. Perbandt, A. B. E. Brauer,
S. Brode, J. P. Frste, C. Betzel and V. A. Erdmann. Acta Cryst. (2007). F63, 46-48.
4. Combinatorial crystallization of an RNA-protein complex. Danielle Bodrero Hoggan, Jeffrey A. Chao, G. S. Prasad, C. David Stouta and James R. Williamson.
Acta Cryst (2003). D59, 466-473
5. Cocrystallizing natural RNA with its unnatural mirror image: biochemical and preliminary X-ray diffraction analysis of a 5S rRNA A-helix racemate.
Volker A. Erdmanna et al. Acta Cryst. (2007). F63, 839843.
Order Information
Each Natrix kit contains 48 unique reagents. To order individual reagents, use Custom Shop

catalog number listed below. Refer to page 36 for further details.


Cat. No. Name Description Price
HR2-116 Natrix 10 ml, tube format $285.00
HR2-918-** Natrix Custom Shop 185 ml $138.00
** = reagent number 1-48
d e s c r i p t i o n
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 20
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Crystal of MtaN, the N-terminal DNA binding domain of
the Multidrug Transporter Activation protein from B. subti-
lis. Initial crystals grown from Natrix reagent number 29.
Courtesy of Michael Godsey.
Oregon Health Sciences University, Dept. of Biochemistry
and Molecular Biology
s u c c e s s s t o r y
N A T R I X

21
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1. 0.01 M Magnesium chloride hexahydrate, 0.05 M MES monohydrate pH 5.6, 1.8 M Lithium sulfate monohydrate
2. 0.01 M Magnesium acetate tetrahydrate, 0.05 M MES monohydrate pH 5.6, 2.5 M Ammonium sulfate
3. 0.1 M Magnesium acetate tetrahydrate, 0.05 M MES monohydrate pH 5.6,
20% v/v (+
/
-)-2-Methyl-2,4-pentanediol
4. 0.2 M Potassium chloride, 0.01 M Magnesium sulfate heptahydrate, 0.05 M MES monohydrate pH 5.6,
10% v/v Polyethylene glycol 400
5. 0.2 M Potassium chloride, 0.01 M Magnesium chloride hexahydrate, 0.05 M MES monohydrate pH 5.6,
5% w/v Polyethylene glycol 8,000
6. 0.1 M Ammonium sulfate, 0.01 M Magnesium chloride hexahydrate, 0.05 M MES monohydrate pH 5.6,
20% w/v Polyethylene glycol 8,000
7. 0.02 M Magnesium chloride hexahydrate, 0.05 M MES monohydrate pH 6.0, 15% v/v 2-Propanol
8. 0.1 M Ammonium acetate, 0.005 M Magnesium sulfate heptahydrate, 0.05 M MES monohydrate pH 6.0,
0.6 M Sodium chloride
9. 0.1 M Potassium chloride, 0.01 M Magnesium chloride hexahydrate, 0.05 M MES monohydrate pH 6.0,
10% v/v Polyethylene glycol 400
10. 0.005 M Magnesium sulfate heptahydrate, 0.05 M MES monohydrate pH 6.0, 5% w/v Polyethylene glycol 4,000
11. 0.01 M Magnesium chloride hexahydrate, 0.05 M Sodium cacodylate trihydrate pH 6.0,
1.0 M Lithium sulfate monohydrate
12. 0.01 M Magnesium sulfate heptahydrate, 0.05 M Sodium cacodylate trihydrate pH 6.0,
1.8 M Lithium sulfate monohydrate
13. 0.015 M Magnesium acetate tetrahydrate, 0.05 M Sodium cacodylate trihydrate pH 6.0, 1.7 M Ammonium sulfate
14. 0.1 M Potassium chloride, 0.025 M Magnesium chloride hexahydrate,
0.05 M Sodium cacodylate trihydrate pH 6.0, 15% v/v 2-Propanol
15. 0.04 M Magnesium chloride hexahydrate, 0.05 M Sodium cacodylate trihydrate pH 6.0,
5% v/v (+
/
-)-2-Methyl-2,4-pentanediol
16. 0.04 M Magnesium acetate tetrahydrate, 0.05 M Sodium cacodylate trihydrate pH 6.0,
30% v/v (+
/
-)-2-Methyl-2,4-pentanediol
17. 0.2 M Potassium chloride, 0.01 M Calcium chloride dihydrate, 0.05 M Sodium cacodylate trihydrate pH 6.0,
10% w/v Polyethylene glycol 4,000
18. 0.01 M Magnesium acetate tetrahydrate, 0.05 M Sodium cacodylate trihydrate pH 6.5,
1.3 M Lithium sulfate monohydrate
19. 0.01 M Magnesium sulfate heptahydrate, 0.05 M Sodium cacodylate trihydrate pH 6.5, 2.0 M Ammonium sulfate
20. 0.1 M Ammonium acetate, 0.015 M Magnesium acetate tetrahydrate, 0.05 M Sodium cacodylate trihydrate pH 6.5,
10% v/v 2-Propanol
21. 0.2 M Potassium chloride, 0.005 M Magnesium chloride hexahydrate, 0.05 M Sodium cacodylate trihydrate pH 6.5,
0.9 M 1,6-Hexanediol
22. 0.08 M Magnesium acetate tetrahydrate, 0.05 M Sodium cacodylate trihydrate pH 6.5,
15% v/v Polyethylene glycol 400
23. 0.2 M Potassium chloride, 0.01 M Magnesium chloride hexahydrate, 0.05 M Sodium cacodylate trihydrate pH 6.5,
10% w/v Polyethylene glycol 4,000
24. 0.2 M Ammonium acetate, 0.01 M Calcium chloride dihydrate, 0.05 M Sodium cacodylate trihydrate pH 6.5,
10% w/v Polyethylene glycol 4,000
25. 0.08 M Magnesium acetate tetrahydrate, 0.05 M Sodium cacodylate trihydrate pH 6.5,
30% w/v Polyethylene glycol 4,000
26. 0.2 M Potassium chloride, 0.1 M Magnesium acetate tetrahydrate, 0.05 M Sodium cacodylate trihydrate pH 6.5,
10 % w/v Polyethylene glycol 8,000
27. 0.2 M Ammonium acetate, 0.01 M Magnesium acetate tetrahydrate, 0.05 M Sodium cacodylate trihydrate pH 6.5,
30% w/v Polyethylene glycol 8,000
28. 0.05 M Magnesium sulfate hydrate, 0.05 M HEPES sodium pH 7.0, 1.6 M Lithium sulfate monohydrate
29. 0.01 M Magnesium chloride hexahydrate, 0.05 M HEPES sodium pH 7.0, 4.0 M Lithium chloride
30. 0.01 M Magnesium chloride hexahydrate, 0.05 M HEPES sodium pH 7.0, 1.6 M Ammonium sulfate
31. 0.005 M Magnesium chloride hexahydrate, 0.05 M HEPES sodium pH 7.0,
25% v/v Polyethylene glycol monomethyl ether 550
32. 0.2 M Potassium chloride, 0.01 M Magnesium chloride hexahydrate, 0.05 M HEPES sodium pH 7.0,
1.7 M 1,6-Hexanediol
33. 0.2 M Ammonium chloride, 0.01 M Magnesium chloride hexahydrate, 0.05 M HEPES sodium pH 7.0,
2.5 M 1,6-Hexanediol
34. 0.1 M Potassium chloride, 0.005 M Magnesium sulfate hydrate, 0.05 M HEPES sodium pH 7.0,
15% v/v (+
/
-)-2-Methyl-2,4-pentanediol
35. 0.1 M Potassium chloride, 0.01 M Magnesium chloride hexahydrate, 0.05 M HEPES sodium pH 7.0,
5% v/v Polyethylene glycol 400
36. 0.1 M Potassium chloride, 0.01 M Calcium chloride dihydrate, 0.05 M HEPES sodium pH 7.0,
10% v/v Polyethylene glycol 400
37. 0.2 M Potassium chloride, 0.025 M Magnesium sulfate hydrate, 0.05 M HEPES sodium pH 7.0,
20% v/v Polyethylene glycol 200
38. 0.2 M Ammonium acetate, 0.15 M Magnesium acetate tetrahydrate, 0.05 M HEPES sodium pH 7.0,
5% w/v Polyethylene glycol 4,000
39. 0.1 M Ammonium acetate, 0.02 M Magnesium chloride hexahydrate, 0.05 M HEPES sodium pH 7.0,
5% w/v Polyethylene glycol 8,000
40. 0.01 M Magnesium chloride hexahydrate, 0.05 M TRIS hydrochloride pH 7.5, 1.6 M Ammonium sulfate
41. 0.1 M Potassium chloride, 0.015 M Magnesium chloride hexahydrate, 0.05 M TRIS hydrochloride pH 7.5
10% v/v Polyethylene glycol monomethyl ether 550
42. 0.01 M Magnesium chloride hexahydrate, 0.05 M TRIS hydrochloride pH 7.5, 5% v/v 2-Propanol
43. 0.05 M Ammonium acetate, 0.01 M Magnesium chloride hexahydrate, 0.05 M TRIS hydrochloride pH 7.5,
10% v/v (+
/
-)-2-Methyl-2,4-pentanediol
44. 0.2 M Potassium chloride, 0.05 M Magnesium chloride hexahydrate, 0.05 M TRIS hydrochloride pH 7.5,
10% w/v Polyethylene glycol 4,000
45. 0.025 M Magnesium sulfate hydrate, 0.05 M TRIS hydrochloride pH 8.5, 1.8 M Ammonium sulfate
46. 0.005 M Magnesium sulfate hydrate, 0.05 M TRIS hydrochloride pH 8.5, 2.9 M 1,6-Hexanediol
47. 0.1 M Potassium chloride, 0.01 M Magnesium chloride hexahydrate, 0.05 M TRIS hydrochloride pH 8.5,
30% v/v Polyethylene glycol 400
48. 0.2 M Ammonium chloride, 0.01 M Calcium chloride dihydrate, 0.05 M TRIS hydrochloride pH 8.5,
30% w/v Polyethylene glycol 4,000
N A T R I X F O R M U L A T I O N
Range from 5.6 to 8.5
p
H
o r g a n i c
p o l y m e r
n o n - v o l a t i l e
o r g a n i c
s a l t
(+/-)-2-Methyl-2,4-pentanediol
1,6-Hexanediol
2-Propanol
Ammonium acetate
Ammonium chloride
Ammonium sulfate
Calcium chloride
Lithium chloride
Lithium sulfate
Magnesium chloride
Magnesium acetate
Magnesium sulfate
Potassium chloride
Polyethylene glycol 200
Polyethylene glycol 400
Polyethylene glycol 4,000
Polyethylene glycol 8,000
Polyethylene glycol MME 550
n a t r i x
f a c t o r s
n
Crystallization screen for proteins, peptides,
& nucleic acids
n
Cryo ready reagents for cryocrystallography
1
n
Sparse matrix formula efficiently samples
salts, polymers, organics, & pH
n
Screen for cryo and crystallization conditions
using only one screen
a p p l i c a t i o n
f e a t u r e s
m e t h o d o l o g i e s
The proportion of macromolecular x-ray
structures solved using cryocrystallographic
methods is increasing at an exponential rate.
The advantages of macromolecular cryocrys-
tallography are numerous. The most widely
used method of flash cooling macromolecu-
lar crystals is to support the crystal in a film of
cryoprotected mother liquor on or in a small
fiber loop which is subsequently cooled in
liquid nitrogen at around 100K. The liquid about the crystal must freeze as an amorphous glass to avoid
crystal damage and diffraction from ordered ice. Cryoprotection is achieved by mixing the mother liquor
and crystal with a suitable cryoprotectant reagent. The most frequently utilized cryoprotectant is glycerol.
Determining a Cryoprotectant
Determining initial and optimal cryoprotectant concentration is often a process of trial and error. One must
find suitable cryoprotectant concentrations which stabilize the crystal while at the same time combine with
the crystallization reagent to form an amorphous glass.
Crystal Screen Cryo removes the guesswork from determining the preliminary glycerol concentration
required to mix with the crystallization reagent to form an amorphous glass. Crystal Screen Cryo reagents
are preformulated with the appropriate amount of glycerol required to form an amorphous glass with
each unique reagent composition. Crystals obtained in Crystal Screen Cryo will already have a suitable
concentration of glycerol in the reagent. This avoids exhaustive empirical searches for preliminary cryopro-
tectant conditions. From the preliminary cryoprotectant conditions, cryoprotectant concentration can be
optimized to minimize mosaic spread and maximize diffraction resolution.
Crystal Screen Cryo is a complete sparse matrix reagent kit designed to provide a rapid screening method
for the crystallization of biological macromolecules in the presence of glycerol, the most frequently utilized
cryoprotectant. Crystal Screen Cryo utilizes the original Crystal Screen protocol but is optimized to
include the appropriate concentration of glycerol required to form an amorphous glass at 100K. The pri-
mary screen variables are salt, pH, precipitant (salts, polymers, volatile organics, and non-volatile organics),
and cryoprotectant concentration. The screen is a straightforward, effective, and practical kit for determin-
ing preliminary crystallization conditions and provides a good starting point for finding suitable cryopro-
tectant conditions for macromolecular crystals grown in a wide range of reagents. Crystal Screen Cryo is
also effective in determining the solubility of a macromolecule in a wide range of precipitants and pH.
Each Crystal Screen Cryo kit contains 50 unique reagents, 10 ml each. Ready-to-use reagents are sterile
filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics,
and buffers.
References
1. Garman, E.F. and Mitchell, E.P., J. Appl. Cryst. (1996) 29, 584- 587.
d e s c r i p t i o n
Order Information
Each Crystal Screen Cryo kit contains 50 unique reagents. To order individual reagents, use
Custom Shop

catalog number listed below. Refer to page 36 for further details.


Cat. No. Name Description Price
HR2-122 Crystal Screen Cryo 10 ml, tube format $285.00
HR2-914-** Crystal Screen Cryo Custom Shop 185 ml $138.00
** = reagent number 1-50
C R Y S T A L S C R E E N C R Y O

Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 22
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The protein crystal above is a LFA-1 I-domain/Inhibitor
complex. Crystallization conditions obtained using
Crystal Screen Cryo from Hampton Research.
Courtesy of Joerg Kallen.
Novartis Pharma AG
s u c c e s s s t o r y
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1. 0.02 M Calcium chloride dihydrate, 0.1 M Sodium acetate trihydrate pH 4.6,
30% v/v (+
/
-)-2-Methyl-2,4-pentanediol
2. 0.26 M Potassium sodium tartrate tetrahydrate, 35% v/v Glycerol
3. 0.26 M Ammonium phosphate monobasic, 35% v/v Glycerol
4. 0.075 M Tris hydrochloride pH 8.5, 1.5 M Ammonium sulfate, 25% v/v Glycerol
5. 0.2 M Sodium citrate tribasic dihydrate, 0.1 M HEPES sodium pH 7.5,
30% v/v (+
/
-)-2-Methyl-2,4-pentanediol
6. 0.16 M Magnesium chloride hexahydrate, 0.08 M Tris hydrochloride pH 8.5,
24% w/v Polyethylene glycol 4,000, 20% v/v Glycerol
7. 0.07 M Sodium cacodylate trihydrate pH 6.5, 0.98 M Sodium acetate trihydrate, 30% v/v Glycerol
8. 0.14 M Sodium citrate tribasic dihydrate, 0.07 M Sodium cacodylate trihydrate pH 6.5,
21% v/v 2-Propanol, 30% v/v Glycerol
9. 0.17 M Ammonium acetate, 0.085 M Sodium citrate tribasic dihydrate pH 5.6,
25.5% w/v Polyethylene glycol 4,000, 15% v/v Glycerol
10. 0.17 M Ammonium acetate, 0.085 M Sodium acetate trihydrate pH 4.6,
25.5% w/v Polyethylene glycol 4,000, 15% v/v Glycerol
11. 0.07 M Sodium citrate tribasic dihydrate pH 5.6, 0.7 M Ammonium phosphate monobasic,
30% v/v Glycerol
12. 0.18 M Magnesium chloride hexahydrate, 0.09 M HEPES sodium pH 7.5, 27% v/v 2-Propanol,
10% v/v Glycerol
13. 0.2 M Sodium citrate tribasic dihydrate, 0.1 M Tris hydrochloride pH 8.5,
30% v/v Polyethylene glycol 400
14. 0.19 M Calcium chloride dihydrate, 0.095 M HEPES sodium pH 7.5, 26.6% v/v Polyethylene glycol 400,
5% v/v Glycerol
15. 0.17 M Ammonium sulfate, 0.085 M Sodium cacodylate trihydrate pH 6.5,
25.5% w/v Polyethylene glycol 8,000, 15% v/v Glycerol
16. 0.075 M HEPES sodium pH 7.5, 1.125 M Lithium sulfate monohydrate, 25% v/v Glycerol
17. 0.17 M Lithium sulfate monohydrate, 0.085 M Tris hydrochloride pH 8.5,
25.5% w/v Polyethylene glycol 4,000, 15% v/v Glycerol
18. 0.16 M Magnesium acetate tetrahydrate, 0.08 M Sodium cacodylate trihydrate pH 6.5,
16% w/v Polyethylene glycol 8,000, 20% v/v Glycerol
19. 0.16 M Ammonium acetate, 0.08 M Tris hydrochloride pH 8.5, 24% v/v 2-Propanol, 20% v/v Glycerol
20. 0.16 M Ammonium sulfate, 0.08 M Sodium acetate trihydrate pH 4.6,
20% w/v Polyethylene glycol 4,000, 20% v/v Glycerol
21. 0.2 M Magnesium acetate tetrahydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5,
30% v/v (+
/
-)-2-Methyl-2,4-pentanediol
22. 0.17 M Sodium acetate trihydrate, 0.085 M Tris hydrochloride pH 8.5,
25.5% w/v Polyethylene glycol 4,000, 15% v/v Glycerol
23. 0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES sodium pH 7.5, 30% v/v Polyethylene glycol 400
24. 0.14 M Calcium chloride dihydrate, 0.07 M Sodium acetate trihydrate pH 4.6, 14% v/v 2-Propanol,
30% v/v Glycerol
25. 0.07 M Imidazole pH 6.5, 0.7 M Sodium acetate trihydrate, 30% v/v Glycerol
26. 0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6,
30% v/v (+
/
-)-2-Methyl-2,4-pentanediol
27. 0.14 M Sodium citrate tribasic dihydrate, 0.07 M HEPES sodium pH 7.5, 14% v/v 2-Propanol,
30% v/v Glycerol
28. 0.17 M Sodium acetate trihydrate, 0.085 M Sodium cacodylate trihydrate pH 6.5,
25.5% w/v Polyethylene glycol 8,000, 15% v/v Glycerol
29. 0.065 M HEPES sodium pH 7.5, 0.52 M Potassium sodium tartrate tetrahydrate, 35% v/v Glycerol
30. 0.17 M Ammonium sulfate, 25.5% w/v Polyethylene glycol 8,000, 15% v/v Glycerol
31. 0.17 M Ammonium sulfate, 25.5% w/v Polyethylene glycol 4,000, 15% v/v Glycerol
32. 1.5 M Ammonium sulfate, 25% v/v Glycerol
33. 3.6 M Sodium formate, 10% v/v Glycerol
34. 0.07 M Sodium acetate trihydrate pH 4.6, 1.4 M Sodium formate, 30% v/v Glycerol
35. 0.075 M HEPES sodium pH 7.5, 0.6 M Sodium phosphate monobasic monohydrate,
0.6 M Potassium phosphate monobasic, 25% v/v Glycerol
36. 0.065 M Tris hydrochloride pH 8.5, 5.2% w/v Polyethylene glycol 8,000, 35% v/v Glycerol
37. 0.07 M Sodium acetate trihydrate pH 4.6, 5.6% w/v Polyethylene glycol 4,000, 30% v/v Glycerol
38. 0.09 M HEPES sodium pH 7.5, 1.26 M Sodium citrate tribasic dihydrate, 10% v/v Glycerol
39. 0.085 M HEPES sodium pH 7.5, 1.7% v/v Polyethylene glycol 400, 1.7 M Ammonium sulfate,
15% v/v Glycerol
40. 0.095 M Sodium citrate tribasic dihydrate pH 5.6, 19% v/v 2-Propanol,
19% w/v Polyethylene glycol 4,000, 5% v/v Glycerol
41. 0.085 M HEPES sodium pH 7.5, 8.5% v/v 2-Propanol, 17% w/v Polyethylene glycol 4,000,
15% v/v Glycerol
42. 0.04 M Potassium phosphate monobasic, 16% w/v Polyethylene glycol 8,000,
20% v/v Glycerol
43. 24% w/v Polyethylene glycol 1,500, 20% v/v Glycerol
44. 0.1 M Magnesium formate dihydrate, 50% v/v Glycerol
45. 0.16 M Zinc acetate dihydrate, 0.08 M Sodium cacodylate trihydrate pH 6.5,
14.4% w/v Polyethylene glycol 8,000, 20% v/v Glycerol
46. 0.16 M Calcium acetate hydrate, 0.08 M Sodium cacodylate trihydrate pH 6.5,
14.4% w/v Polyethylene glycol 8,000, 20% v/v Glycerol
47. 0.08 M Sodium acetate trihydrate pH 4.6, 1.6 M Ammonium sulfate, 20% v/v Glycerol
48. 0.08 M Tris hydrochloride pH 8.5, 1.6 M Ammonium phosphate monobasic, 20% v/v Glycerol
49. 0.8 M Lithium sulfate monohydrate, 1.6% w/v Polyethylene glycol 8,000, 20% v/v Glycerol
50. 0.4 M Lithium sulfate monohydrate, 12% w/v Polyethylene glycol 8,000, 20% v/v Glycerol
C R Y S TA L S C R E E N C R Y O F O R M U L AT I O N
Range from 4.0 to 9.0
p
H
o r g a n i c
p o l y m e r
n o n - v o l a t i l e
o r g a n i c
s a l t
(+/-)-2-Methyl-2,4-pentanediol
2-Propanol
Ammonium acetate
Ammonium phosphate
Ammonium sulfate
Calcium acetate
Calcium chloride
Lithium sulfate
Magnesium acetate
Magnesium chloride
Magnesium formate
Potassium phosphate
K/Na tartrate
Sodium acetate
Sodium citrate
Sodium formate
Sodium phosphate
Zinc acetate
Polyethylene glycol 400
Polyethylene glycol 1,500
Polyethylene glycol 4,000
Polyethylene glycol 8,000
b u f f e r
HEPES sodium
Imidazole
Sodium acetate
Sodium cacodylate
Sodium citrate
TRIS hydrochloride
Glycerol
c r y o p r o c t e c a n t
crystal screen cryo
f a c t o r s
Crystal of RNA tetraloop.
Courtesy of Imre Berger and Li Su.
MIT
s u c c e s s s t o r y
n
Crystallization screen for nucleic acid
fragments
n
Screen a matrix of pH, polyamine, & ions
n
Can be used with ribozymes, pseudoknots,
RNA, & DNA
n
Uses MPD as the primary precipitant
a p p l i c a t i o n
f e a t u r e s
The Nucleic Acid Mini Screen is an efficient
screen formulated to assist in the determi-
nation of preliminary crystallization condi-
tions of nucleic acid fragments.
1
The for-
mulation is based upon the publication, A
Highly Efficient 24 Condition Matrix for the
Crystallization of Nucleic Acid Fragments
where the preliminary crystallization condi-
tions of 35 nucleic acids were determined
utilizing this formulation.
1
Samples include
DNA-Drug complexes, C-Tetrad and G-Quartet Motifs, RNA oligomers, and others. By using 1 to 4 mM
oligonucleotide stock concentration, the screen requires less than 100 l of sample. The screen is typically
performed at 4C although room temperature incubations can also be performed. To evaluate the effect of
equilibration kinetics as well as initial and final sample concentrations, the screens are typically performed
using the hanging or sitting drop vapor diffusion method with two drops on a slide (1 l + 2 l and 2 l +
2 l), side by side. The 24 well format of the mini screen provides for a fast setup and uses small amounts of
sample. This makes it possible to cost-effectively screen many sequences as well as variations of a particular
sequence. The composition of the Nucleic Acid Mini Screen allows one to apply the formulation to other
nucleic acids such as deoxy- and ribozymes, pseudoknots, and tRNAs.
Each Nucleic Acid Mini Screen kit contains 24 unique reagents, 1.0 ml each plus a 250 ml volume of dehy-
drant (35% v/v MPD). All solutions are sterile filtered and formulated with ultra-pure Type 1 water.
References
1. Berger, et al., A Highly Effective 24 Condition Matrix for the Crystallization of Nucleic Acid Fragments. Acta Cryst. Section D.
(1996) Vol. D52 Part 3, 465-468.
2. Adams, A., Nucleic Acids Research (2002) Vol. 30, 719-725.
3. Crystallization and preliminary X-ray diffraction analysis of an Escherichia coli tRNAGly acceptor-stem microhelix. C. Frster, M. Perbandt,
A. B. E. Brauer, S. Brode, J. P. Frste, C. Betzel and V. A. Erdmann. Acta Cryst. (2007). F63, 46-48.
d e s c r i p t i o n
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 24
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N U C L E I C A C I D M I N I S C R E E N

Order Information
Each Nucleic Acid Mini Screen kit contains 24 unique reagents plus dehydrant.
Cat. No. Name Description Price
HR2-118 Nucleic Acid Mini Screen 1 ml, tube format + 250 ml bottle $195.00
5.5 to 7.0
using
40 mM Sodium
cacodylate
pH
35% v/v MPD
Lithium
Sodium
Potassium
Magnesium
Strontium
Barium
chlorides
Cobalt hexamine
Spermine tetra-HCl
polyamines
+
+
2
+
+
2
dehydrant
Tube # Precipitant
1. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
2. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
3. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
4. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
5. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
6. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
7. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
8. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
9. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
10. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
11. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
12. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
13. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
14. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
15. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
16. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
17. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
18. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
19. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
20. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
21. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
22. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
23. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
24. 10% v/v (+/-)-2-Methyl-2,4-pentanediol
Tube # Buffer
1. 40 mM Sodium cacodylate pH 5.5
2. 40 mM Sodium cacodylate pH 5.5
3. 40 mM Sodium cacodylate pH 5.5
4. 40 mM Sodium cacodylate pH 5.5
5. 40 mM Sodium cacodylate pH 6.0
6. 40 mM Sodium cacodylate pH 6.0
7. 40 mM Sodium cacodylate pH 6.0
8. 40 mM Sodium cacodylate pH 6.0
9. 40 mM Sodium cacodylate pH 6.0
10. 40 mM Sodium cacodylate pH 6.0
11. 40 mM Sodium cacodylate pH 6.0
12. 40 mM Sodium cacodylate pH 6.0
13. 40 mM Sodium cacodylate pH 6.0
14. 40 mM Sodium cacodylate pH 7.0
15. 40 mM Sodium cacodylate pH 7.0
16. 40 mM Sodium cacodylate pH 7.0
17. 40 mM Sodium cacodylate pH 7.0
18. 40 mM Sodium cacodylate pH 7.0
19. 40 mM Sodium cacodylate pH 7.0
20. 40 mM Sodium cacodylate pH 7.0
21. 40 mM Sodium cacodylate pH 7.0
22. 40 mM Sodium cacodylate pH 7.0
23. 40 mM Sodium cacodylate pH 7.0
24. 40 mM Sodium cacodylate pH 7.0
Tube # Polyamine
1. 20 mM Cobalt hexamine
2. 20 mM Cobalt hexamine
3. 20 mM Cobalt hexamine
4. 20 mM Cobalt hexamine
5. 12 mM Spermine tetrahydrochloride
6. 12 mM Spermine tetrahydrochloride
7. 12 mM Spermine tetrahydrochloride
8. 12 mM Spermine tetrahydrochloride
9. 12 mM Spermine tetrahydrochloride
10. 12 mM Spermine tetrahydrochloride
11. 12 mM Spermine tetrahydrochloride
12. 12 mM Spermine tetrahydrochloride
13. 12 mM Spermine tetrahydrochloride
14. 12 mM Spermine tetrahydrochloride
15. 12 mM Spermine tetrahydrochloride
16. 12 mM Spermine tetrahydrochloride
17. 12 mM Spermine tetrahydrochloride
18. 12 mM Spermine tetrahydrochloride
19. 12 mM Spermine tetrahydrochloride
20. 12 mM Spermine tetrahydrochloride
21. 12 mM Spermine tetrahydrochloride
22. 12 mM Spermine tetrahydrochloride
23. 12 mM Spermine tetrahydrochloride
24. 12 mM Spermine tetrahydrochloride
Tube # Monovalent Ion
1. None
2. 80 mM Sodium chloride
3. 12 mM Sodium chloride,
80 mM Potassium chloride
4. 40 mM Lithium chloride
5. 80 mM Potassium chloride
6. 80 mM Potassium chloride
7. 80 mM Sodium chloride
8. 80 mM Sodium chloride
9. 80 mM Sodium chloride,
12 mM Potassium chloride
10. 12 mM Sodium chloride,
80 mM Potassium chloride
11. 80 mM Sodium chloride
12. 80 mM Potassium chloride
13. None
14. 80 mM Potassium chloride
15. 80 mM Potassium chloride
16. 80 mM Sodium chloride
17. 80 mM Sodium chloride
18. 80 mM Sodium chloride,
12 mM Potassium chloride
19. 12 mM Sodium chloride,
80 mM Potassium chloride
20. 80 mM Sodium chloride
21. 80 mM Potassium chloride
22. 40 mM Lithium chloride
23. 40 mM Lithium chloride
24. None
Tube # Divalent Ion
1. 20 mM Magnesium chloride
2. 20 mM Magnesium chloride
3. None
4. 20 mM Magnesium chloride
5. 20 mM Magnesium chloride
6. None
7. 20 mM Magnesium chloride
8. None
9. 20 mM Magnesium chloride
10. None
11. 20 mM Barium chloride
12. 20 mM Barium chloride
13. 80 mM Strontium chloride
14. 20 mM Magnesium chloride
15. None
16. 20 mM Magnesium chloride
17. None
18. 20 mM Magnesium chloride
19. None
20. 20 mM Barium chloride
21. 20 mM Barium chloride
22. 80 mM Strontium chloride,
20 mM Magnesium chloride
23. 80 mM Strontium chloride
24. 80 mM Strontium chloride,
20 mM Magnesium chloride
N U C L E I C A C I D M I N I S C R E E N F O R M U L A T I O N
Crystal is that of the intact canine lymphoma
antibody. Preliminary crystallization conditions
determined using the Low Ionic Strength Screen.
Courtesy of Lisa Harris.
University of California, Irvine
s u c c e s s s t o r y
n
Crystallization screen for intact monoclonal
antibodies, monoclonal antibody fragments,
& proteins less soluble at low ionic strength
n
Screen a complete pH profile (2-12) at low
ionic strength
n
Enhanced temperature effects due to low
ionic strength
n
Monodisperse PEG 3350
a p p l i c a t i o n
f e a t u r e s
The Low Ionic Strength Screen is effective for
determining the preliminary crystallization
conditions of intact monoclonal antibodies
1
.
However, this screen is not just an intact
antibody screen. The screen has effectively
determined the preliminary crystallization
conditions for numerous monoclonal anti-
body fragments as well as other soluble
proteins. The screen should be utilized as a
low ionic strength crystallization screen for
proteins where this strategy could be effective in determining preliminary crystallization screens.
In this screen, the concentration of a high purity, monodisperse PEG 3,350 is varied from 4 to 24% w/v (4, 8,
12, 16, 20 and 24% w/v) versus a pH range of 2 to 12 (2, 3, 3.5, 4, 4.5, 5, 5.5, 6.0, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10,
11, 12). Stock buffer concentrations are 50 mM. Final buffer concentration in the drop is typically 10 mM.
Unique features of this screen: (1) Low ionic strength. Buffer concentration is supplied as 50 mM, result-
ing in an initial drop concentration of 10 mM (Drop = 4 l sample, 2 l buffer, 5 l precipitant); (2)
The Polyethylene glycol (PEG) 3,350 is a special, high-purity, monodisperse preparation with an Mr of
3300-3400. Most PEGs of this molecular weight have an Mr of plus or minus 500 rather than 50; (3) An
extremely broad range of pH 2 to 12 is sampled. At low ionic strength, the effects of pH and temperature
upon sample solubility are amplified. Hence this screen allows one to critically evaluate the effects of tem-
perature and pH upon sample solubility and crystallization. It is recommended the screen be repeated at
several temperatures between 4C and 37C to take advantage of the low ionic strength feature.
The format of this screen is different than other screens offered by Hampton Research. The screen is sup-
plied as a set of eighteen 50 mM buffers, six PEG 3,350 concentrations, and a single dehydrant, PEG 3,350.
This formulation allows one to screen up to 108 conditions if desired. The formulation is designed to offer
options. Coarse sample of pH and/or precipitant concentration can be performed with 24 or even 12 drops.
This will eliminate large regions of sampling space and conserve sample. Subsequent screens and perhaps
even optimization can encompass an increasingly finer grid matrix of pH, precipitant concentration, as well
as other variables significant for sample crystallization. The protocol requires the following pipetting steps
for a typical vapor diffusion experiment: (1) Pipet dehydrant to reservoir; (2) Pipet drop; (3) Pipet buffer
to drop; (4) Pipet precipitant to drop.
Each kit contains 24 unique reagents of varying pH and PEG 3,350 concentration. Buffers of 50 mM are sup-
plied for pH 2, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 11, and 12 in 1 ml volumes. The precipitant
Polyethylene glycol 3,350 is supplied as 4, 8, 12, 16, 20, and 24% w/v in 1 ml volumes. Dehydrant is supplied
as 250 ml of 24% PEG 3,350. Combining each pH and precipitant supplied can generate 108 crystallization
screening conditions. All solutions are sterile filtered and formulated with ultra-pure Type 1 water.
References
1. Harris, et al., Crystallization of intact monoclonal antibodies, Proteins: Structure, Function, and Genetics (1995) 23:285-289.
Order Information
Each Low Ionic Strength Screen kit contains 24 unique reagents plus dehydrant.
Cat. No. Name Description Price
HR2-120 Low Ionic Strength Screen 1 ml, tube format + 250 ml bottle $195.00
d e s c r i p t i o n
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l o w I o n I C s T r e n G T H s C r e e n

Precipitant
4% w/v Polyethylene glycol 3,350
8% w/v Polyethylene glycol 3,350
12% w/v Polyethylene glycol 3,350
16% w/v Polyethylene glycol 3,350
20% w/v Polyethylene glycol 3,350
24% w/v Polyethylene glycol 3,350
Buffer
0.05 M Potassium chloride pH 2.0
0.05 M Citric acid pH 3.0
0.05 M Citric acid pH 3.5
0.05 M Citric acid pH 4.0
0.05 M Citric acid pH 4.5
0.05 M Citric acid pH 5.0
0.05 M Citric acid pH 5.5
0.05 M MES monohydrate pH 6.0
0.05 M BIS-TRIS pH 6.5
0.05 M Imidazole pH 7.0
0.05 M HEPES pH 7.5
0.05 M Tris pH 8.0
0.05 M Tris pH 8.5
0.05 M Glycine pH 9.0
0.05 M Glycine pH 9.5
0.05 M Glycine pH 10.0
0.05 M Sodium phosphate dibasic pH 11.0
0.05 M Sodium phosphate dibasic pH 12.0
Dehydrant
24% w/v Polyethylene glycol 3,350
Low Ionic Strength
Screen Components
n
Primary, secondary or orthoganol crystallization
screen for biological macromolecules
n
For use with soluble proteins and membrane
proteins
n
A methodology to uncover different crystal
forms
n
Optimization screening in conjunction with
preliminary crystallization conditions
n
ThermoFluor

assays
n
Solubility, folding and stability studies
n
Developed at Hampton Research
n
Screens a portfolio of small molecules for their
ability to establish stabilizing, intermolecular,
hydrogen bonding, hydrophobic and electro-
static interactions which could promote lattice
formation and crystallization

n
Organic salts & acids

n
Biologically active small molecules

n
Amino acids and peptide

n
Macromolecular digests
n
Modular screen design allows Silver Bullets to
be screened against virtually any crystallization
reagent
n
Original & Bio Formulations
n
A combinatorial library of more than 1,090
chemicals, of which more than 400 are unique
n
Tube or Deep Well block format
a p p l i c a t i o n s
f e a t u r e s
An alternative approach to macromolecular crystallization.
The Silver Bullets formulation and methodology screens
validated small molecule libraries against a few select crys-
tallization reagents as an effective, orthogonal approach
to macromolecular crystallization. The formulation offers
an alternative and efficient approach that is an excellent
complement to current crystallization method-ologies.
Silver Bullets is a library of small molecules that have been
shown to promote crystal lattice formation. X-ray diffraction analysis has demonstrated the reagents have
the ability to:
Stabilize the conformation of the protein
Perturb the interaction of the protein with the solvent
Participate in forming important lattice contacts
Build the crystal lattice by forming reversible cross-links between the macromolecules in the crystal
Results with the Silver Bullets approach have been very encouraging, with more than twice as many
proteins being crystallized overall as were crystallized from controls free of any small molecules.
1-3
There
have been frequent occasions where some exceptional result has been obtained for specific proteins,
and numerous examples where new or unusual crystal forms were obtained. X-ray diffraction analysis has
revealed the small molecules in the crystal lattice, involved at the centers of hydrogen bonding networks
and electrostatic interaction.
3
Silver Bullets can be used as a primary crystallization screen; as a secondary or orthogonal screen to pro-
duce crystals when traditional screens are not successful; as an optimization screen in conjunction with
preliminary crystallization conditions; as a methodology to uncover different crystal forms; to promote
intramolecular interactions that may stabilize a macromolecules conformation and promote crystallization.
Silver Bullets solutions are designed for use with the Silver Bullets PEG/Tacsimate Crystallization Reagents
but may also be used with other crystallization reagents.
The Silver Bullets kits are composed of 96 solutions in either screw cap tubes or a single Deep Well block
(Greiner 786261) HT format. Each reagent is a mixture of small molecules or macromolecular digest in
0.02 M HEPES sodium pH 6.8 buffer. Each Silver Bullets solution is supplied in a 0.25 ml volume. Each
solution contains between 2 and 20 small molecules. Silver Bullets reagents include: Organic salts and acids
Biologically active molecules Amino acids and peptides Macromolecular digests. Silver Bullets Bio
reagents include: Small organic molecules, organic salts, and organic acids Biologically active molecules,
co-factors, and ligands Amino acids, peptides, nucleotides, drugs, and carbohydrates Biochemical
pathway intermediates.
References
1. Searching for silver bullets: An alternative strategy for crystallizing macromolecules. Alexander McPherson and Bob Cudney. Journal of Structural Biology
156 (2006) 387-406.
2. A novel strategy for the crystallization of proteins: X-ray diffraction validation. Steven B. Larson, John S. Day, Robert Cudney, and Alexander McPherson.
Acta Cryst. (2007) D63, 310-318.
3. Development of an alternative approach to protein crystallization. McPherson, Alexander; Nguyen, Chieniang; Larson, Steven B; Day, John S; Cudney,
Bob. J Struct Funct Genomics, Volume 8, Number 4, December 2007, 193-198.
d e s c r i p t i o n
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 26
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S I L V E R B U L L E T S

s I l v e r b u l l e T s b I o

Mellitic acid, a Silver Bullet for Trypsin


Order Information
Silver Bullets and Silver Bullets Bio kits each contain 96 unique reagents. To order individual
reagents, use Custom Shop

catalog numbers listed below. Refer to page 36 for further details.


Cat. No. Name Description Price
HR2-078 Silver Bullets 0.25 ml, tube format $490.00
HR2-096 Silver Bullets HT 0.25 ml, Deep Well block format $490.00
HR2-080 Silver Bullets Bio 0.25 ml, tube format $490.00
HR2-088 Silver Bullets Bio HT 0.25 ml, Deep Well block format $490.00
HR2-090 PEG/Tacsimate pH 5.8 1 ml, Deep Well block format $185.00
HR2-092 PEG/Tacsimate pH 6.8 1 ml, Deep Well block format $185.00
HR2-094 PEG/Tacsimate pH 7.8 1 ml, Deep Well block format $185.00
HR2-996-** Silver Bullets Custom Shop 0.25 ml $65.00
HR2-988-** Silver Bullets Bio Custom Shop 0.25 ml $65.00

** = reagent number 1-96
27
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organi c salts
organi c aci ds
compati ble wi th a di verse
array of reagents
f a c t o r s
silver bullets

macromolecular di gests
ami no aci ds
pepti des
combi natori al
formulati on
combi natori al
formulati on
bi ologi cally acti ve small molecules
ami no aci ds
pepti des
nucleoti des
carbohydrates
small organi c molecules
organi c salts
organi c aci ds
compati ble wi th a di verse
array of reagents
bi ologi cally acti ve molecules co- factors li gands
f a c t o r s
engage in multiple, strong hydrogen bonds within the protein
form hydrophobic interactions with the protein
become involved in electrostatic interactions with the protein
enhance, disrupt and alter the pattern of clustering in the critical
nucleus of the crystal
develop stabilizing lattice interactions within the crystal
serve as transient stabilizing intermediaries
c o m p o s e d o f c o m p o u n d s w i t h t h e a b i l i t y t o
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 28
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n
Manipulate sample-sample & sample-solvent
interactions to enhance or alter sample
solubility
n
18 classes of reagents
n
Highly concentrated (10x) reagent formulation
n
96 unique reagents
n
Crystallization or sample solubility
optimization
n
Tube or Deep Well block format
a p p l i c a t i o n
f e a t u r e s
Additive Screen is a library of small molecules
that can affect the solubility and crystalliza-
tion of biological macromolecules, including
both soluble and membrane proteins. These
small molecules can perturb and manipulate
sample-sample and sample-solvent interac-
tions, as well as perturb water structure
which can alter and improve both the solubil-
ity and crystallization of a sample. Additives
can stabilize or engender conformity by spe-
cific interaction with the macromolecules. There are numerous reports of the use of additives to improve
the quality and size of macromolecular crystals.
1-5

Additive Screen contains 96 unique reagents, 1 ml each, tube format.
Additive Screen HT contains 96 unique reagents, 1 ml each, in a single Deep Well block format.
References
1. Crystallization of membrane proteins., Edited by Hartmut Michel, CRC Press (1991).
2. Crystallization of Nucleic Acids and Proteins: A Practical Approach., Edited by A. Ducruix and R. Giege, Oxford University Press (1992).
3. Cudney, R., et al., Screening and optimization strategies for macromolecular crystal growth., Acta Cryst. (1994) D50, 414-423.
4. Sousa R., Use of glycerol, polyols and other protein structure stabilizing agents in protein crystallization., Acta Cryst. (1995) D51, 271-277.
5. Trakhanov, S. and Quiocho, F.A., Influence of divalent cations on protein crystallization., Protein Science (1995) 4, 9, 1914-1919.
Order Information
Each Additive Screen kit contains 96 unique reagents. To order individual reagents, use Custom
Shop

catalog number listed below. Refer to page 36 for further details.


Cat. No. Name Description Price
HR2-428 Additive Screen 1 ml, tube format $490.00
HR2-138 Additive Screen HT 1 ml, Deep Well block format $490.00
HR2-428-** Additive Screen Custom Shop 1 ml $65.00
** = reagent number 1-96
d e s c r i p t i o n
Initial crystals grown using Crystal Screen Reagent
Number 9. Crystals were approximately 100 microns
in size. With the addition of Dextran Sulfate from the
Additive Screen kit, larger crystals were produced as
pictured above (approximately 250 microns).
Courtesy of Ranjit K. Deka.
University of Texas Southwestern Medical Center
s u c c e s s s t o r y
A D D I T I V E S C R E E N

a d d I T I v e s C r e e n H T

Tube # Additive
73. (G1) 30% w/v Trimethylamine N-oxide dihydrate
74. (G2) 30% w/v 1,6-Hexanediol
75. (G3) 30% v/v (+/-)-2-Methyl-2,4-pentanediol
76. (G4) 50% v/v Polyethylene glycol 400
77. (G5) 50% v/v Jeffamine M-600

pH 7.0
78. (G6) 40% v/v 2,5-Hexanediol
79. (G7) 40% v/v ()-1,3-Butanediol
80. (G8) 40% v/v Polypropylene glycol P 400
81. (G9) 30% v/v 1,4-Dioxane
82. (G10) 30% v/v Ethanol
83. (G11) 30% v/v 2-Propanol
84. (G12) 30% v/v Methanol
85. (H1) 40% v/v 1,4-Butanediol
86. (H2) 40% v/v tert-Butanol
87. (H3) 40% v/v 1,3-Propanediol
88. (H4) 40% v/v Acetonitrile
89. (H5) 40% v/v Formamide
90. (H6) 40% v/v 1-Propanol
91. (H7) 5% v/v Ethyl acetate
92. (H8) 40% v/v Acetone
93. (H9) 0.25% v/v Dichloromethane
94. (H10) 7% v/v 1-Butanol
95. (H11) 40% v/v 2,2,2-Trifluoroethanol
96. (H12) 40% v/v 1,1,1,3,3,3-Hexafluoro-2-propanol
A D D I T I V E S C R E E N F O R M U L A T I O N
Tube # Additive
1. (A1) 0.1 M Barium chloride dihydrate
2. (A2) 0.1 M Cadmium chloride hydrate
3. (A3) 0.1 M Calcium chloride dihydrate
4. (A4) 0.1 M Cobalt(II) chloride hexahydrate
5. (A5) 0.1 M Copper(II) chloride dihydrate
6. (A6) 0.1 M Magnesium chloride hexahydrate
7. (A7) 0.1 M Manganese(II) chloride tetrahydrate
8. (A8) 0.1 M Strontium chloride hexahydrate
9. (A9) 0.1 M Yttrium(II) chloride hexahydrate
10. (A10) 0.1 M Zinc chloride
11. (A11) 0.1 M Iron(III) chloride hexahydrate
12. (A12) 0.1 M Nickel(II) chloride hexahydrate
13. (B1) 0.1 M Chromium(III) chloride hexahydrate
14. (B2) 0.1 M Praseodymium(III) acetate hydrate
15. (B3) 1.0 M Ammonium sulfate
16. (B4) 1.0 M Potassium chloride
17. (B5) 1.0 M Lithium chloride
18. (B6) 2.0 M Sodium chloride
19. (B7) 0.5 M Sodium fluoride
20. (B8) 1.0 M Sodium iodide
21. (B9) 2.0 M Sodium thiocyanate
22. (B10) 1.0 M Potassium sodium tartrate tetrahydrate
23. (B11) 1.0 M Sodium citrate tribasic dihydrate
24. (B12) 1.0 M Cesium chloride
Tube # Additive
25. (C1) 1.0 M Sodium malonate pH 7.0
26. (C2) 0.1 M L-Proline
27. (C3) 0.1 M Phenol
28. (C4) 30% v/v Dimethyl sulfoxide
29. (C5) 0.1 M Sodium bromide
30. (C6) 30% w/v 6-Aminohexanoic acid
31. (C7) 30% w/v 1,5-Diaminopentane dihydrochloride
32. (C8) 30% w/v 1,6-Diaminohexane
33. (C9) 30% w/v 1,8-Diaminooctane
34. (C10) 1.0 M Glycine
35. (C11) 0.3 M Glycyl-glycyl-glycine
36. (C12) 0.1 M Taurine
37. (D1) 0.1 M Betaine hydrochloride
38. (D2) 0.1 M Spermidine
39. (D3) 0.1 M Spermine tetrahydrochloride
40. (D4) 0.1 M Hexammine cobalt(III) chloride
41. (D5) 0.1 M Sarcosine
42. (D6) 0.1 M Trimethylamine hydrochloride
43. (D7) 1.0 M Guanidine hydrochloride
44. (D8) 0.1 M Urea
45. (D9) 0.1 M b-Nicotinamide adenine dinucleotide
hyd.
46. (D10) 0.1 M Adenosine-5'-triphosphate disodium salt
47. (D11) 0.1 M TCEP hydrochloride
48. (D12) 0.01 M GSH (L-Glutathione reduced),
0.01 M GSSG (L-Glutathione oxidized)
Tube # Additive
49. (E1) 0.1 M EDTA sodium salt
50. (E2) 5% w/v Polyvinylpyrrolidone K15
51. (E3) 30% w/v Dextran sulfate sodium salt (Mr 5,000)
52. (E4) 40% v/v Pentaerythritol ethoxylate (3/4 EO/OH)
53. (E5) 10% w/v Polyethylene glycol 3,350
54. (E6) 30% w/v D(+)-Glucose monohydrate
55. (E7) 30% w/v Sucrose
56. (E8) 30% w/v Xylitol
57. (E9) 30% w/v D-Sorbitol
58. (E10) 12% w/v myo-Inositol
59. (E11) 30% w/v D-(+)-Trehalose dihydrate
60. (E12) 30% w/v D-(+)-Galactose
61. (F1) 30% v/v Ethylene glycol
62. (F2) 30% v/v Glycerol
63. (F3) 3.0 M NDSB-195
64. (F4) 2.0 M NDSB-201
65. (F5) 2.0 M NDSB-211
66. (F6) 2.0 M NDSB-221
67. (F7) 1.0 M NDSB-256
68. (F8) 0.15 mM CYMAL

-7
69. (F9) 20% w/v Benzamidine hydrochloride hydrate
70. (F10) 5% w/v n-dodecyl-N,N-dimethylamine-N-oxide
71. (F11) 5% w/v n-Octyl-b-D-glucoside
72. (F12) 5% w/v n-Dodecyl-b-D-maltoside
Concentrations are actual formulations.
29
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a p p l i c a t i o n
f e a t u r e s
Detergent Screen kits are designed to allow the
rapid and convenient evaluation of 96 unique
detergent reagents for their ability to influence
the solubility and crystallization of the sample.
These screens are designed to be compatible
with most popular crystallization reagents.
The detergents in these kits are capable of
manipulating hydrophobic sample-sample inter-
actions which can lead to non-specific aggre-
gation, and prevent or interfere with sample
crystallization. The detergents also perturb water
structure which may play a role in sample crystal-
lization.
Non-specific aggregation is a common deterrent
to the crystallization of soluble macromolecules
as well as membrane proteins. There is exten-
sive literature demonstrating the effectiveness of including detergents in the crystallization trial towards
preventing non-specific aggregation due to hydrophobic interactions and hence improving crystallization.
1
The kits allow one to screen the most effective detergents used in crystal growth.
The Detergent Screen kits are preformulated so that simple pipetting is all that is required to screen the
detergents with the hanging or sitting drop vapor diffusion technique. Screens with these kits are usually
performed after preliminary crystallization conditions have been determined, although ab initio screens
are also practical. The kits are recommended for both soluble and membrane proteins where non-specific
aggregation is a suspected complication or where one simply wishes to screen detergents as an optimiza-
tion variable. The screens are suitable for hanging drop, sitting drop, microbatch, free interface diffusion
and sandwich drop crystallization methodologies.
Each Detergent Screen kit contains 96 unique reagents. 0.25 ml of detergent solution is preformulated at
10 times the reported CMC (unless otherwise noted) in sterile filtered water.
References
1. Crystallization of membrane proteins. Edited by Hartmut Michel, CRC Press, (1991).
Order Information
Each Detergent Screen kit contains 96 unique reagents. To order individual reagents, use Custom Shop


catalog number listed below. Refer to page 36 for further details.
Cat. No. Name Description Price
HR2-408 Detergent Screen 0.25 ml, tube format $490.00
HR2-406 Detergent Screen HT 0.25 ml, Deep Well block format $490.00
HR2-406-** Detergent Screen Custom Shop 0.5 ml $65.00
** = reagent number 1-96
d e s c r i p t i o n
n
Prevent and manipulate non-specific
aggregation due to hydrophobic interactions
n
Kits include popular detergents used in the
crystallization of membrane proteins

n
Ionic detergents

n
Non-ionic detergents

n
Zwitterionic detergents

n
Non-detergent Sulfobetaines

n
Synthetic lipids
n
Useful with soluble proteins where
hydrophobic interactions limit sample solubility
n
Detergents are compatible with microbatch
under oil crystallization, as well as vapor
diffusion, free interface diffusion, & dialysis
D E T E R G E N T S C R E E N

d e T e r G e n T s C r e e n H T

Detergent molecule interacting with beta-lactamase.


Crystal above is that of -Lactamase. Crystal was
grown using 15% w/v PEG 6000, 50 mM HEPES
pH 7.0, and in the presence of CYMAL-5 and
CYMAL-6 detergent from the Hampton Research
Detergent Screen kit 1.
Courtesy of James Knox.
University of Connecticut,
Department of Molecular and Cell Biology
s u c c e s s s t o r y
D E T E R G E N T S C R E E N F O R M U L A T I O N
Well # Detergent CMC (mM)
1. (A1)
2. (A2)
3. (A3)
4. (A4)
5. (A5)
6. (A6)
7. (A7)
8. (A8)
9. (A9)
10. (A10)
11. (A11)
12. (A12)
13. (B1)
14. (B2)
15. (B3)
16. (B4)
17. (B5)
18. (B6)
19. (B7)
20. (B8)
21. (B9)
22. (B10)
23. (B11)
24. (B12)
BAM
n-Dodecyl-b-iminodipropionic acid,
monosodium salt
Dodecyltrimethylammonium chloride
CTAB
Deoxycholic acid, sodium salt
Sodium dodecyl sulfate
Sodium cholate
Sodium dodecanoyl sarcosine
ANAPOE

-X-305
IPTG
n-Hexadecyl-b-D-maltoside
ANAPOE

-58
n-Tetradecyl-b-D-maltoside
ANAPOE

-80
n-Tridecyl-b-D-maltoside
ANAPOE

-C12E9
ANAPOE

-20
Thesit

ANAPOE

-35
ANAPOE

-C13E8
ANAPOE

-C12E8
n-Dodecyl-b-D-maltoside
CYMAL

-7
ANAPOE

-X-114
None
None
0.046
1.0
6.0
8.27
14.0
14.4
None
None
0.0006
0.004
0.010
0.012
0.033
0.05
0.059
0.09
0.091
0.10
0.11
0.17
0.19
0.20
Well # Detergent CMC (mM)
25. (C1)
26. (C2)
27. (C3)
28. (C4)
29. (C5)
30. (C6)
31. (C7)
32. (C8)
33. (C9)
34. (C10)
35. (C11)
36. (C12)
37. (D1)
38. (D2)
39. (D3)
40. (D4)
41. (D5)
42. (D6)
43. (D7)
44. (D8)
45. (D9)
46. (D10)
47. (D11)
48. (D12)
ANAPOE

-C12E10
Sucrose monolaurate
CYMAL

-6
n-Undecyl-b-D-maltoside
ANAPOE

-X-405
TRITON

X-100
ANAPOE

-C10E6
n-Decyl-b-D-thiomaltoside
Octyl maltoside, fluorinated
ANAPOE

-C10E9
Big CHAP, deoxy
n-Decyl-b-D-maltoside
LDAO
n-Decanoylsucrose
n-Nonyl-b-D-thioglucoside
n-Nonyl-b-D-thiomaltoside
CYMAL

-5
n-Nonyl-b-D-maltoside
n-Nonyl-b-D-glucoside
HEGA

-10
MEGA -10
C8E5
CYMAL

-4
C8E4
0.20
0.30
0.56
0.59
0.81
0.90
0.90
0.90
1.02
1.3
1.4
1.8
2.0
2.5
2.9
3.2
5.0
6.0
6.5
7.0
7.0
7.1
7.6
8.0
Well # Detergent CMC (mM)
73. (G1)
74. (G2)
75. (G3)
76. (G4)
77. (G5)
78. (G6)
79. (G7)
80. (G8)
81. (G9)
82. (G10)
83. (G11)
84. (G12)
85. (H1)
86. (H2)
87. (H3)
88. (H4)
89. (H5)
90. (H6)
91. (H7)
92. (H8)
93. (H9)
94. (H10)
95. (H11)
96. (H12)
NDSB-195
NDSB-201
NDSB-211
NDSB-221
NDSB-256
ZWITTERGENT

3-14
n-Dodecyl-N,N-dimethylglycine
FOS-Choline

-12
FOS-Choline

-8, fluorinated
n-Undecyl-N,N-Dimethlamine-oxide
ZWITTERGENT

3-12
DDMAB
FOS-MEA

-10
CHAPS
CHAPSO
FOS-Choline

-10
n-Decyl-N,N-dimethylglycine
FOS-Choline

-9
ZWITTERGENT

3-10
CYCLOFOS
TM
-3
FOS-Choline

-8
ZWITTERGENT

3-08
LysoFos
TM
Choline 12
LysoFos
TM
Choline 10
N/A
N/A
N/A
N/A
N/A
0.40
1.5
1.5
2.2
3.21
4.0
4.3
5.25
8.0
8.0
11.0
19.0
39.5
40.0
43.0
114.0
330.0
0.7
7.0
Well # Detergent CMC (mM)
49. (E1)
50. (E2)
51. (E3)
52. (E4)
53. (E5)
54. (E6)
55. (E7)
56. (E8)
57. (E9)
58. (E10)
59. (E11)
60. (E12)
61. (F1)
62. (F2)
63. (F3)
64. (F4)
65. (F5)
66. (F6)
67. (F7)
68. (F8)
69. (F9)
70. (F10)
71. (F11)
72. (F12)
n-Octyl-b-D-thiomaltoside
n-Octyl-b-D-thioglucoside
Hexaethylene glycol monooctyl ether
DDAO
C-HEGA

-11
Pluronic

F-68
HECAMEG

n-Octyl-b-D-glucoside
n-Octanoylsucrose
MEGA-9
2,6-Dimethyl-4-heptyl-b-D-malto-pyranoside
n-Heptyl-b-D-thioglucopyranoside
n-Octyl-b-D-galactopyranoside
CYMAL

-3
C-HEGA

-10
HEGA

-9
Dimethyloctylphosphine oxide
MEGA-8
C-HEGA

-9
HEGA

-8
CYMAL

-2
n-Hexyl-b-D-glucopyranoside
C-HEGA

-8
CYMAL

-1
8.5
9.0
10.0
10.4
11.5
17.9
19.5
20.0
24.4
25.0
27.5
29.0
29.5
34.5
35.0
39.0
40.0
79.0
108.0
109.0
120.0
250.0
277.0
340.0
Iridium Compounds
H
1.008 1
Li
6.940 3
Na
22.99 11
K
39.09 19
Ca
40.08 20
Sc
45.10 21
Ti
47.90 22
V
50.95 23
Cr
52.01 24
Mn
54.93 25
Fe
55.84 26
Co
58.94 27
Ni
58.69 28
Cu
63.57 29
Zn
65.38 30
Ga
69.72 31
Ge
72.60 32
As
74.91 33
Se
78.96 34
Br
79.916 35
Kr
83.70 36
Mg
24.32 12
Be
9.02 4
Ar
39.944 18
Cl
35.457 17
S
32.06 16
P
30.98 15
Si
28.06 14
Al
26.97 13
B
10.82 5
C
12.01 6
N
14.008 7
O
16.00 8
F
19.00 9
Ne
20.183 10
He
4.003 2
Rb
85.48 37
Sr
87.63 38
Y
88.92 39
Zr
91.22 40
Nb
92.91 41
Mo
95.95 42
Tc
97.907 43
Ru
101.70 44
Rh
102.91 45
Pd
106.70 46
Ag
107.88 47
Cd
112.41 48
In
114.76 49
Sn
118.70 50
Sb
121.76 51
Te
127.61 52
I
126.92 53
Xe
131.30 54
Cs
132.91 55
Ba
137.36 56
La
138.92 57
Hf
178.60 72
Ta
180.88 73
W
183.92 74
Re
186.31 75
Os
190.20 76
Ir
193.10 77
Pt
195.23 78
Au
197.20 79
Hg
200.61 80
Tl
204.39 81
Pb
207.21 82
Bi
209.00 83
Po
208.98 84
At
209.99 85
Rn
222.02 86
Fr
223.02 87
Ra
226.03 88
Ac
227.03 89 90
to
103
58
to
71
Lanthanides
Actinides
Ce
140.13 58
Pr
140.92 59
Nd
144.27 60
Pm
144.91 61
Sm
150.43 62
Eu
152.00 63
Gd
156.90 64
Tb
159.20 65
Dy
162.46 66
Ho
164.94 67
Er
167.20 68
Tm
169.40 69
Yb
173.04 70
Lu
175.00 71
Th
232.12 90
Pa
231.04 91
U
238.07 92
Np
237.05 93
Pu
244.06 94
Am
243.06 95
Cm
247.07 96
Bk
247.07 97
Cf
242.06 98
Es
252.08 99
Fm
257.10 100
Md
258.10 101
No
259.10 102
Lr
260.11 103
Osmium Compounds
Silver Compounds
Platinum Compounds
Mercury Compounds Thallium Compounds
Lead Compounds Gold Compounds
Cadmium Compounds
Tungsten Compounds
Samarium Compounds
Lanthanum Compounds
Europium Compounds Gadolinium Compounds
Ytterbium Compounds
Dysprosium Compounds
Praseodymium Compounds
Neodymium Compounds Holmium Compounds
Rhenium Compounds
Lutetium Compounds
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 30
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Heavy atoms for multiple isomorphous
replacement
n
Convenient sets of popular heavy atoms
n
Each kit includes a heavy atom tutorial and
formulation guide
a p p l i c a t i o n
f e a t u r e s
A Heavy Atom Screen kit provides 50 mg
of each heavy atom in an o-ring screw cap
micro tube. This convenient format provides
sufficient material for the preparation of
numerous (15 x 100 mM or 1,500 x 1 mM)
small volume (100 l) fresh stock solutions
of heavy atoms. The quantity is sufficient for
screening and derivatization but avoids the
problem of storing large quantities of heavy
atoms. Individual heavy atoms from each
kit are available in larger quantities on a special order basis for researchers requiring larger amounts of
material.
References
1. Heavy-atom derivatization. Elspeth Garman and James W. Murray, Acta Cryst. (2003). D59, 1903-1913.
2. Towards a rational approach for heavy-atom derivative screening in protein crystallography. Johnson Agniswamy, M. Gordon Joyce, Carl H. Hammer,
and Peter D. Sun. Acta Cryst. (2008) D64, 354-367.
3. Screening for phasing atoms in protein crystallography. Titus J Boggon and Lawrence Shapiro. Structure 2000, Vol 8 No 7, 143-149.
d e s c r i p t i o n
H e a v y a T o M s C r e e n s
Protein crystal with the heavy atom derivative
Potassium osmate.
Kimberly J. Skinner, Structural Biology and Biophysics,
Pfizer Global R&D, Sandwich, Kent, United Kingdom.
s u c c e s s s t o r y
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Order Information
Each Heavy Atom Screen Pt kit contains 12 unique reagents. To order individual
reagents, use Custom Shop

catalog number listed below. Refer to page 36 for


further details.
Cat. No. Name Description Price
HR2-442 Heavy Atom Screen Pt 50 mg, tube format $418.00
HR2-442-** Heavy Atom Screen Pt 100 mg $80.00
Custom Shop
** = reagent number 1-12
Order Information
Each Heavy Atom Screen Au kit contains 6 unique reagents. To order individual
reagents, use Custom Shop

catalog number listed below. Refer to page 36 for


further details.
Cat. No. Name Description Price
HR2-444 Heavy Atom Screen Au 50 mg, tube format $275.00
HR2-444-** Heavy Atom Screen Au 100 mg $80.00
Custom Shop
** = reagent number 1-6
Order Information
Each Heavy Atom Screen Hg kit contains 15 unique reagents. To order individual
reagents, use Custom Shop

catalog number listed below. Refer to page 36 for


further details.
Cat. No. Name Description Price
HR2-446 Heavy Atom Screen Hg 50 mg, tube format $314.00
HR2-446-** Heavy Atom Screen Hg 100 mg $80.00
Custom Shop
** = reagent number 1-15
Heavy Atom Screen Pt (Platinum)
1. Potassium tetrachloroplatinate(II)
2. Ammonium tetrachloroplatinate(II)
3. Potassium hexachloroplatinate(IV)
4. Potassium tetranitroplatinate(II)
5. Potassium tetracyanoplatinate(II) hydrate
6. Dichloro(ethylenediamine)platinum(II)
7. Diammino Platinum Dinitrite
8. Potassium tetrabromoplatinate(II)
9. Potassium hexabromoplatinate(IV)
10. Platinum potassium iodide
11. Platinum potassium thiocyanate
12. Di--iodobis(ethylenediamine)diplatinum(II) nitrate
Heavy Atom Screen Au (Gold)
1. Gold(I) potassium cyanide
2. Potassium tetrachloroaurate(III) hydrate
3. Sodium tetrachloroaurate(III) dihydrate
4. Gold(III) chloride
5. Hydrogen tetrachloroaurate(III) trihydrate
6. Potassium tetrabromoaurate(III) dihydrate
Heavy Atom Screen Hg (Mercury)
1. Mersalyl acid
2. Ethyl Mercuric Phosphate
3. Mercury(II) chloride
4. Mercury(II) acetate
5. Ethylmercurithiosalicylic acid, sodium salt
6. Phenylmercury acetate
7. Mercury(II) potassium iodide
8. p-Chloromercuribenzoic acid
9. Ethylmercury chloride
10. Mercury(II) bromide
11. Mercury(II) iodide
12. Mercury(II) nitrate monohydrate
13. Mercury(II) cyanide
14. Mercury(II) oxide, yellow
15. Tetrakis(acetoxymercuri)methane
Heavy Atom Screen M2
1. Sodium tungstate dihydrate
2. Ammonium tetrathiotungstate(VI)
3. Samarium(III) chloride hexahydrate
4. Samarium(III) acetate hydrate
5. Samarium(III) nitrate hexahydrate
6. Lanthanum(III) nitrate hexahydrate
7. Europium(III) nitrate hexahydrate
8. Europium(III) chloride hexahydrate
9. Gadolinium(III) chloride hydrate
10. Lutetium(III) chloride hexahydrate
11. Lutetium(III) acetate hydrate
12. Ytterbium(III) chloride hydrate
13. Dysprosium(III) chloride hexahydrate
14. Praseodymium(III) chloride heptahydrate
15. Neodymium(III) chloride hydrate
16. Holmium(III) chloride hexahydrate
17. Potassium hexachlororhenate(IV)
18. Potassium perrhenate
Order Information
Each Heavy Atom Screen M1 kit contains 19 unique reagents. To order individual
reagents, use Custom Shop

catalog number listed below. Refer to page 36 for


further details.
Cat. No. Name Description Price
HR2-448 Heavy Atom Screen M1 50 mg, tube format $343.00
HR2-448-** Heavy Atom Screen M1 100 mg $80.00
Custom Shop
** = reagent number 1-19
Order Information
Each Heavy Atom Screen M2 kit contains 18 unique reagents. To order individual
reagents, use Custom Shop

catalog number listed below. Refer to page 36 for


further details.
Cat. No. Name Description Price
HR2-450 Heavy Atom Screen M2 50 mg, tube format $320.00
HR2-450-** Heavy Atom Screen M2 100 mg $80.00
Custom Shop
** = reagent number 1-18
Heavy Atom Screen M1
1. Thallium(III) chloride hydrate
2. Thallium(I) chloride
3. Thallium(III) acetate hydrate
4. Lead(II) acetate trihydrate
5. Lead(II) nitrate
6. Lead(II) chloride
7. Silver nitrate
8. Cadmium chloride hydrate
9. Cadmium iodide
10. Potassium hexachloroiridate(IV)
11. Iridium(III) chloride hydrate
12. Sodium hexachloroiridate(III) hydrate
13. Ammonium hexachloroiridate(III) hydrate
14. Potassium hexanitroiridium(III)
15. Potassium osmate(VI) dihydrate
16. Ammonium hexabromoosmate(IV)
17. Potassium hexachloroosmate(IV)
18. Osmium(III) chloride hydrate
19. Acetoxytrimethyllead(IV)
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 32
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Produce heavy atom derivative of biological
macromolecules for phasing
n
I3C kit for soaking or cocrystallization
n
Phasing using SAD or SIRAS
n
Ready-to-use, no weighing required
a p p l i c a t i o n
f e a t u r e s
I3C can be used for heavy atom derivatization
of biological macromolecules for subsequent
single wavelength anomalous dispersion
(SAD) or single isomorphous replacement
plus anomalous scattering (SIRAS).
The two carboxylic acid groups and one
amino group of I3C can interact by way of
hydrogen bonds with both the backbone and
side chains of proteins. This can result in a
relatively highoccupancy of the bound ligand
I3C. The three iodine atoms per I3C mol-
ecule provide for a strong anomalous signal.
The three iodine atoms in I3C form an equilateral triangle with 6.0 side lengths. These triangular struc-
tures can readily be identified in the anomalous electron density map.
Kit Contents
12 aliquots of I3C (280 mg each) (5-Amino-2,4,6-triiodoisophthalic acid CAS number 35453-19-1)
12 aliquots of 2.0 M Lithium hydroxide (650 l each)
User Guide
References
1. A magic triangle for experimental phasing of macromolecules. Beck et al. Acta Cryst. D64, 1179, 2008.
2. Structure determination of the cancer-associated Mycoplasma hyorhinis protein Mhp37. Sippel et al. Acta Cryst. D64, 1172, 2008.
3. 5-Amino-2,4,6 triiodo-isophthalic acid monohydrate. Beck et al. Acta Cryst. E64:1286, 2008.
Order Information
Cat. No. Name Description Price
HR3-133 I3C Phasing Kit 24 tubes $155.00
d e s c r i p t i o n
1 3 C P H a s I n G k I T

Crystal of cytochrome P450 Cyp121 from Mycobacterium tuberculosis with red color due
to the ferric heme iron. Crystal diffracts to 1.4Ang at Proxima1/Soleil, France.
Pascal Belin, iBiTec-s/CEA, Saclay, France.
Crystal of human phosphatase.
Chris Gray at the Institute of Cancer Research,
London, United Kingdom.
Crystal Image.
Allan DArcy, Switzerland.
Crystals of apo kinase.
Initial hit from Hampton Research Grid Screen Salt HT.
Michelle Quiles and Annie Hassell,
GlaxoSmithKline, RTP, North Carolina, USA.
c u s t o m s h o p
c r y s t a l l i z a t i o n
r e a g e n t s
Protein crystal grown with additive.
Ranjit Deka, University of Texas Southwest Medical Center, USA.
T A B L E O F C O N T E N T S
36 - 37 c u s t o m s h o p

c r y s t a l l i z a t i o n r e a g e n t s
P A G E S
C R Y S T A L S C R E E N L I T E

Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
The Hampton Research Custom Shop delivers ready-to-use crystallization
reagents for screening, optimizing and producing crystals. The reagents are
formulated at the time of order, using the same production protocol, chemi-
cals, and quality control used when making the original Hampton Research
crystallization kit.
Formulating the Custom Shop reagents at the time of order ensures consis-
tency as well as saves time and offers convenience. They are shipped within 48
hours. Custom Shop reagents may not be returned for refund or exchange.
To order Custom Shop reagents, use the catalog numbers listed below. If you
have any questions, please feel free to contact Customer Service.
When reproducing and optimizing reagent conditions, please be sure to also
consider the Hampton Research Optimize and StockOptions reagents
and kits.
d e s c r i p t i o n
Heavy Atom Pt (Platinum) - 100 mg $80.00
Catalog Number: HR2-442-**
** = reagent number 1-12
For example, reagent number 1 = HR2-442-01
Additive Screen - 1.0 ml $65.00
Catalog Number: HR2-428-**
** = reagent number 1-96
For example, reagent number A1 = HR2-428-01
Crystal Screen - 185 ml $138.00
Catalog Number: HR2-910-**
** = reagent number 1-50
For example, reagent number 1 = HR2-910-01
Crystal Screen Cryo - 185 ml $138.00
Catalog Number: HR2-914-**
** = reagent number 1-50
For example, reagent number 1 = HR2-914-01
Crystal Screen Lite - 185 ml $138.00
Catalog Number: HR2-916-**
** = reagent number 1-50
For example, reagent number 1 = HR2-916-01
Grid Screen PEG/LiCl - 185 ml $138.00
Catalog Number: HR2-928-**
** = reagent number A1-D6
For example, reagent number A1 = HR2-928-A1
Grid Screen Sodium Chloride - 185 ml $138.00
Catalog Number: HR2-932-**
** = reagent number A1-D6
For example, reagent number A1 = HR2-932-A1
Grid Screen Sodium Malonate - 185 ml $138.00
Catalog Number: HR2-947-**
** = reagent number A1-D6
For example, reagent number A1 = HR2-947-A1
Crystal Screen 2 - 185 ml $138.00
Catalog Number: HR2-912-**
** = reagent number 1-48
For example, reagent number 1 = HR2-912-01
Heavy Atom Au (Gold) - 100 mg $80.00
Catalog Number: HR2-444-**
** = reagent number 1-6
For example, reagent number 1 = HR2-444-01
Heavy Atom Hg (Mercury) - 100 mg $80.00
Catalog Number: HR2-446-**
** = reagent number 1-16
For example, reagent number 1 = HR2-446-01
Heavy Atom M1 - 100 mg $80.00
Catalog Number: HR2-448-**
** = reagent number 1-19
For example, reagent number 1 = HR2-448-01
Heavy Atom M2 - 100 mg $80.00
Catalog Number: HR2-450-**
** = reagent number 1-18
For example, reagent number 1 = HR2-450-01
Index - 185 ml $138.00
Catalog Number: HR2-944-**
** = reagent number 1-96
For example, reagent number 1 = HR2-944-01
Quik Screen - 185 ml $138.00
Catalog Number: HR2-921-**
** = reagent number A1-D6
For example, reagent number A1 = HR2-921-A1
MembFac - 185 ml $138.00
Catalog Number: HR2-920-**
** = reagent number 1-48
For example, reagent number 1 = HR2-920-01
Natrix - 185 ml $138.00
Catalog Number: HR2-918-**
** = reagent number 1-48
For example, reagent number 1 = HR2-918-01
PCT - 185 ml $138.00
Catalog Number: HR2-940-**
** = reagent number A1-B2
For example, reagent number A1 = HR2-940-A1
PEG/Ion Screen - 185 ml $138.00
Catalog Number: HR2-922-**
** = reagent number 1-48
For example, reagent number 1 = HR2-922-01
PEG/Ion Screen 2 - 185 ml $138.00
Catalog Number: HR2-998-**
** = reagent number 1-48
For example, reagent number 1 = HR2-998-01
PEGRx 1 - 185 ml $138.00
Catalog Number: HR2-982-**
** = reagent number 1-48
For example, reagent number 1 = HR2-982-01
PEGRx 2 - 185 ml $138.00
Catalog Number: HR2-984-**
** = reagent number 1-48
For example, reagent number 1 = HR2-984-01
SaltRx 1 - 185 ml $138.00
Catalog Number: HR2-907-**
** = reagent number 1-48
For example, reagent number 1 = HR2-907-01
SaltRx 2 - 185 ml $138.00
Catalog Number: HR2-909-**
** = reagent number 1-48
For example, reagent number 1 = HR2-909-01
Silver Bullets - 0.25 ml $65.00
Catalog Number: HR2-996-**
** = reagent number 1-96
For example, reagent number A1 = HR2-996-01
Silver Bullets Bio - 0.25 ml $65.00
Catalog Number: HR2-988-**
** = reagent number 1-96
For example, reagent number A1 = HR2-988-01
Detergent Screen - 0.5 ml $65.00
Catalog Number: HR2-406-**
** = reagent number 1-96
For example, reagent number A1 = HR2-406-01
Grid Screen MPD - 185 ml $138.00
Catalog Number: HR2-930-**
** = reagent number A1-D6
For example, reagent number A1 = HR2-930-A1
Grid Screen PEG 6000 - 185 ml $138.00
Catalog Number: HR2-926-**
** = reagent number A1-D6
For example, reagent number A1 = HR2-926-A1
C u s T o M s H o P

C R Y S T A L L I Z A T I O N R E A G E N T S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 36
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Grid Screen Ammonium Sulfate - 185 ml $138.00
Catalog Number: HR2-924-**
** = reagent number A1-D6
For example, reagent number A1 = HR2-924-A1
StockOptions ADA - 185 ml $208.00
Catalog Number: HR2-997-**
** = reagent number 1-13
For example, reagent number 1 = HR2-998-01
StockOptions Imidazole - 185 ml $162.00
Catalog Number: HR2-995-**
** = reagent number 1-17
For example, reagent number 1 = HR2-995-01
StockOptions MES - 185 ml $138.00
Catalog Number: HR2-943-**
** = reagent number 1-20
For example, reagent number 1 = HR2-943-01
StockOptions pH - 185 ml
Catalog Number: HR2-941-**
Reagents 1-39 $139.00
Reagents 40-45 $151.00
** = reagent number 1-45
For example, reagent number 1 = HR2-941-01
StockOptions Sodium Acetate - 185 ml $150.00
Catalog Number: HR2-933-**
** = reagent number 1-21
For example, reagent number 1 = HR2-933-01
StockOptions Sodium Cacodylate - 185 ml $221.00
Catalog Number: HR2-939-**
** = reagent number 1-24
For example, reagent number 1 = HR2-939-01
StockOptions Sodium Citrate - 185 ml $173.00
Catalog Number: HR2-935-**
** = reagent number 1-24
For example, reagent number 1 = HR2-935-01
StockOptions Sodium HEPES - 185 ml $190.00
Catalog Number: HR2-931-**
** = reagent number 1-15
For example, reagent number 1 = HR2-931-01
StockOptions Tris - 185 ml $169.00
Catalog Number: HR2-900-**
** = reagent number 1-21
For example, reagent number 1 = HR2-900-01
StockOptions Tris Hydrochloride - 185 ml $139.00
Catalog Number: HR2-937-**
** = reagent number 1-21
For example, reagent number 1 = HR2-937-01
StockOptions Bicine - 185 ml $139.00
Catalog Number: HR2-999-**
** = reagent number 1-15
For example, reagent number 1 = HR2-999-01
StockOptions Bis-Tris - 185 ml $151.00
Catalog Number: HR2-906-**
** = reagent number 1-21
For example, reagent number 1 = HR2-906-01
StockOptions Bis-Tris Propane - 185 ml $201.00
Catalog Number: HR2-993-**
** = reagent number 1-33
For example, reagent number 1 = HR2-993-01
StockOptions Citric Acid - 185 ml $144.00
Catalog Number: HR2-904-**
** = reagent number 1-44
For example, reagent number 1 = HR2-904-01
StockOptions HEPES - 185 ml $172.00
Catalog Number: HR2-902-**
** = reagent number 1-15
For example, reagent number 1 = HR2-902-01
37
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Crystals growing on a bubble.

Michelle Dechene, North Carolina State University, North Carolina, USA.
Here is a recipe to try:
Mosaicity is about 0.5
t0 0.6
Reagent:
Crystal Screen Cryo
Reagent 23
Mix equal amounts of
Glucose Isomerase
and reagent. Vapor
diffusion method.

Mount crystal in
CryoLoop.

Mosaicity may be
a bit more in this
reagent and the
unit cell will shrink a
o p t i m i z e


c r y s t a l l i z a t i o n g r a d e
r e a g e n t s
Protein crystal.
Alexey Rak, Max-Planck-Institut fur Molekulare Physiologie, Department of Physical Biochemistry, Dortmund, Germany.
P r e - C r y s t a l l i z a t i o n T e s t
I n d e x
S a l t R x
P E G / I o n S c r e e n
C r y s t a l S c r e e n
C r y s t a l S c r e e n 2
C r y s t a l S c r e e n L i t e
C r y s t a l S c r e e n C r y o
G r i d S c r e e n s
q u i k s c r e e n & q u i k o p t i m i z e
L o w I o n i c S t r e n g t h S c r e e n
N u c l e i c A c i d M i n i S c r e e n
N a t r i x
M e m b f a c
S t o c k o p t i o n s S c r e e n s
A d d i t i v e S c r e e n
D e t e r g e n t S c r e e n
H e a v y a t o m s c r e e n s
C r y o p r o - C r y o p r o t e c t a n t
XX
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T A B L E O F C O N T E N T S
40 - 42 o p t i m i z e

- p o l y m e r s
42 o p t i m i z e

- o r g a n i c s ( v o l a t i l e )
42 o p t i m i z e

- o r g a n i c s ( n o n - v o l a t i l e )
43 - 49 o p t i m i z e

- s a l t s
50 - 52 o p t i m i z e

- b u f f e r s
52 o p t i m i z e

- s o l u b i l i z i n g a g e n t s ( n d s b )
53 o p t i m i z e

- r e d u c i n g a g e n t
54 o p t i m i z e

- c r y o p r o t e c t a n t s
55 - 56 o p t i m i z e

- o i l s
57 o p t i m i z e

- s i l i c a h y d r o g e l k i t
57 o p t i m i z e

- l m a g a r o s e
58 o p t i m i z e

- i z i t c r y s t a l d y e

P A G E S
n
Crystallization grade reagents for formulating
screens or for optimization
n
Quantitive formulation
n
Lot to lot consistency
n
Synergistic with Hampton Research
screens & kits
n
Quality and convenience
n
Sterile filtered
a p p l i c a t i o n
f e a t u r e s
Optimize reagents are quantitatively formulated
macromolecular crystallization grade reagents designed
specifically for the crystallization of proteins, peptides,
and nucleic acids. Each Optimize solution is formulated
using high purity salts, polymers, and buffers. Sterile
filtered Optimize reagents are formulated at convenient,
ready to use concentrations. Optimize reagents remove
the guesswork and make the process of reproducing
preliminary screening conditions and general optimiza-
tion faster, easier, and more convenient. When using
Optimize reagents, a scientist moves directly from
the screen to the optimization with no time wasted
searching for and formulating salts, buffers, and viscous
polymers. Each Optimize reagent is supplied with a
Certificate of Analysis as documentation of reagent qual-
ity and performance. Optimize buffer reagents are sup-
plied with calibrated titration curves to make titration
easy, fast, and accurate.
d e s c r i p t i o n
P O L Y M E R S
Ethylene imine polymer
HR2-599 50% solution 200 ml $78.00
Pentaerythritol ethoxylate (15/4 EO/OH)
HR2-745 50% v/v solution 200 ml $54.00
Pentaerythritol propoxylate (5/4 PO/OH)
HR2-739 50% v/v solution 200 ml $54.00
Synonyms: PEI or Poly(ethylene imine) or
Poly(ethyleneimine) solution or Ethyleneimine polymer solution
M
r
600,000 - 1,000,000 CAS [9002-98-6] EC No 205-793-9
Density: 1.08 g/mL at 20C Measured pH Range: 12.0 - 12.1 at 25C
Refractive Index n20/D: 1.450 Conductivity Range: 244.3 - 257.0 mS at 25C
Synonyms: Pentaerythritol ethoxylate (3/4 EO/OH)
C(CH2(OCH2CH2)nOH)4, N ~3.75 Average M
n
~797
CAS [30599-15-6] EC No 500-071-2
Density: 1.2 g/mL at 25C (lit.)
Measured pH Range: 5.2 - 6.0 at 25C
Refractive Index Range: 1.41401 - 1.41578 at 20C
Conductivity Range: 4.2 - 11.4 mS at 25C
Jeffamine

M-600

HR2-501 50% v/v solution pH 7.0 200 ml $94.00


HR2-503 100% solution (untitrated pH >12) 200 ml $94.00
Synonyms: O-(2-Aminopropyl)-O'-(2-methoxyethyl)polypropylene
glycol 500 or Polypropylene glycol 500 mono-2-aminoethyl
mono-2-methoxyethyl ether CH3OCH2CH2O[CH(CH3)CH2O]nCH2CH(NH2)
M
r
600 CAS [77110-54-4] Density: 0.983 g/mL at 20C
HR2-501: Refractive Index Range: 1.40754 - 1.40988 at 20C
Conductivity Range: 8.8 - 10.3 mS at 25C
HR2-503: Refractive Index Range: 1.44501 - 1.44546 at 20C
Conductivity Range: 0.0 - 0.1 mS at 25C
Synonyms: Pentaerythritol ethoxylate (15/4 EO/OH)
C(CH2(OCH2CH2)nOH)2 Average M
n
~270
CAS [30599-15-6] EC No 500-071-2
Density: 1.2 g/mL at 25C (lit.)
Measured pH Range: 7.5 - 8.7 at 25C
Refractive Index: 1.39719 at 20C
Conductivity Range: 37.8 - 40.5 mS at 25C
Pentaerythritol ethoxylate (3/4 EO/OH)
HR2-743 40% v/v solution 200 ml $46.00
Synonyms: Pentaerythritol propoxylate
C[CH2[OCH2CH(CH3)]nOH]4, N ~5 Average M
n
~426
CAS [9051-49-4] EC No 500-030-9
Density of 100% solution: 1.05 g/mL at 25C
Measured pH Range: 7.7 - 8.3 at 25C
Refractive Index: 1.40424 - 1.40433 at 20C
Conductivity Range: 3.8 - 4.7 mS at 25C
Synonyms: Pentaerythritol propoxylate
C(CH2(OCH2CH(CH3))nOH)4, N = 2.1 Average M
n
~629
CAS [9051-49-4] EC No 500-030-9
Density: 1.05 g/mL at 25C (lit.)
Measured pH Range: 4.3 - 4.6 at 25C
Refractive Index: 1.40418 at 20C
Conductivity Range: 6.1 - 6.9 mS at 25C
Synonyms: Poly(sodium acrylate) or Sodium polyacrylate
C3H3NaO2 M
r
94.04 CAS [9003-04-7]
Measured pH Range: 6.4 - 6.9 at 25C
Refractive Index Range: 1.40716 - 1.40821 at 20C Conductivity Range: 30.5 - 41.1 mS at 25C
Synonyms: PEG 200 H(OCH2CH2)nOH Mr 190 - 210
CAS [25322-68-3] EC No 500-038-2
Density: 1.124 g/mL at 20C
Measured pH Range: 8.0 - 10.2 at 25C
Refractive Index Range: 1.46008 - 1.46083 at 20C Conductivity Range: 2.5 - 2.8 mS at 25C
Pentaerythritol propoxylate (17/8 PO/OH)
HR2-741 50% v/v solution 200 ml $58.00
Poly(acrylic acid sodium salt) 5,100
HR2-773 50% w/v solution 200 ml $95.00
Polyethylene glycol 200
HR2-601 100% solution 200 ml $30.00
O P T I M I Z E


- R E A G E N T S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 40
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Jeffamine

ED-2001 pH 7.0
HR2-597 50% w/v solution pH 7.0 200 ml $98.00
Synonyms: O,O'-Bis(2-aminopropyl) polypropylene
glycol-block-polyethylene glycol-block-polypropylene
glycol 1,900 CH3CH(NH2)CH2(OCH2CH2)nOCH2CH(NH2)CH3
M
r
~2,000 CAS [65605-36-9]
Refractive Index Range: 1.40637 - 1.40666 at 20C
Conductivity Range: 4.0 - 5.7 mS at 25C
pH before titration with Hydrochloric acid is > 10
Final measured pH of HR2-597 is 7.0
CH3 CH3 CH3
H2NCHCH2 NH2 (OCHCH2)a (OCH2CH2)b (OCH2CH)c
b 38.7, (a+c) 6.0
CH3 CH3 CH3
O O
x y z
O H2N NH2
( ( ( (((
y 39, (x+z) 6
41
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Polyethylene glycol 1,500
Polyethylene glycol 400
HR2-603 100% solution 200 ml $53.00
Polyethylene glycol 1,000
HR2-523 50% w/v solution 200 ml $90.00
Peroxides (as H2O2) 0.001%
Al ........................... 0.0005%
As ........................ 0.00001%
Ba .......................... 0.0005%
Bi ........................... 0.0005%
Ca ............................ 0.001%
Cd .......................... 0.0005%
Cl ............................. 0.005%
Co .......................... 0.0005%
Cr ........................... 0.0005%
Cu .......................... 0.0005%
Fe .......................... 0.0005%
K ................................ 0.02%
Li ............................ 0.0005%
Mg .......................... 0.0005%
Mn .......................... 0.0005%
Mo .......................... 0.0005%
Na .............................. 0.02%
Ni ........................... 0.0005%
Pb .......................... 0.0005%
SO4 .......................... 0.005%
Sr ........................... 0.0005%
Zn .......................... 0.0005%
HR2-525 50% w/v solution 200 ml $60.00
Polyethylene glycol 3,350 Monodisperse
HR2-591 flake 500 g $118.00
HR2-527 50% w/v solution 200 ml $80.00
HR2-837 25% w/v PEG 3,350, 0.1 M MES pH 5.8 100 ml $95.00
HR2-841 25% w/v PEG 3,350, 0.1 M HEPES Na pH 6.8 100 ml $95.00
HR2-845 25% w/v PEG 3,350, 0.1 M BIS-TRIS pH 7.8 100 ml $95.00
HR2-849 25% w/v PEG 3,350,
0.1 M BIS-TRIS propane pH 7.8 100 ml $95.00
P O L Y M E R S
Synonyms: PEG 400 H(OCH2CH2)nOH Mr 380 - 420
CAS [25322-68-3] EC No 500-038-2 Density: 1.126 g/mL at 20C
Measured pH Range: 9.1 - 9.7 at 25C
Refractive Index Range: 1.46686 - 1.46761 at 20C
Conductivity Range: 2.5 - 2.8 mS at 25C
Synonyms: PEG 1,000 H(OCH2CH2)nOH Mr 950 - 1050
CAS [25322-68-3] EC No 500-038-2
Measured pH Range: 6.5 - 7.7 at 25C
Refractive Index Range: 1.40078 - 1.40104 at 20C
Conductivity Range: 23.1 - 37.5 mS at 25C
Synonyms: PEG 3,350 H(OCH2CH2)nOH Mr 3,300 - 3,400
CAS [25322-68-3] EC No 500-038-2
HR2-527: Measured pH Range: 7.9 - 9.7 at 25C
Refractive Index Range: 1.40164 - 1.40208 at 20C
Conductivity Range: 44.0 - 56.5 mS at 25C
HR2-837: Measured Conductivity: 826 mS at 25C
HR2-841: Measured Refractive Index: 1.37193 at 20C
Measured Conductivity: 4.4 mS at 25C
HR2-845: Measured Conductivity: 379 mS at 25C
HR2-849: Measured Conductivity: 3.7 mS at 25C
Polyethylene glycol 4,000
HR2-605 flake 500 g $80.00
HR2-529 50% w/v solution 200 ml $80.00
Synonyms: PEG 4,000 H(OCH2CH2)nOH Mr 3,500 - 4,500
CAS [25322-68-3] EC No 500-038-2
HR2-529: Measured pH Range: 6.9 - 9.5 at 25C
Refractive Index Range: 1.40198 - 1.40279 at 20C
Conductivity Range: 38.6 - 46.9 mS at 25C
Polyethylene glycol monomethyl ether 550
HR2-611 100% solution 200 ml $85.00
Synonyms: Methoxypolyethylene glycol or mono-Methyl polyethylene glycol or PEG MME 550
CH3O(CH2CH2O)nH Mr 470 - 530 CAS [9004-74-4] EC No 215-801-2
Purity > 100.0% Density: 1.089 g/mL at 25C (lit.) Measured pH Range: 8.5 - 10.5 at 25C
Refractive Index Range: 1.46144 - 1.46187 at 20C Conductivity Range: 0.3 - 0.5 mS at 25C
Polyethylene glycol monomethyl ether 2,000
HR2-613 50% w/v solution 200 ml $85.00
Synonyms: Methoxypolyethylene glycol 2,000 or
mono-Methyl polyethylene glycol 2,000 or PEG MME 2,000
CH3O(CH2CH2O)nH CAS [9004-74-4] EC No 215-801-2
Purity > 95.0% Density: 1.094 g/mL at 25C (lit.) Measured pH Range: 7.0 - 8.8 at 25C
Refractive Index Range: 1.40135 - 1.40161 at 20C Conductivity Range: 28.0 - 60.2 mS at 25C
Synonyms: PEG 1,500 H(OCH
2CH2)nOH Mr 1,400 - 1,600
CAS [25322-68-3] EC No 500-038-2
Measured pH Range: 6.1 - 8.7 at 25C
Refractive Index Range: 1.40117 - 1.40127 at 20C
Conductivity Range: 39.7 - 48.5 mS at 25C
Polyethylene glycol 6,000
HR2-513 flake 500 g $122.00
HR2-533 50% w/v solution 200 ml $80.00
DNases .............. none detected
RNases .............. none detected
Proteases .......... none detected
Phosphatases .... none detected
Peroxides (H2O2) ..... 0.001%
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl .............................. 0.005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ................................. 0.02%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Na ............................... 0.02%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO
4
........................... 0.005%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
Synonyms: PEG 6,000 H(OCH2CH2)nOH Mr 5,000 - 7,000
CAS [25322-68-3] EC No 500-038-2
HR2-533: Measured pH Range: 6.2 - 9.1 at 25C
Refractive Index Range: 1.40209 - 1.40249 at 20C
Conductivity Range: 32.5 - 39.6 mS at 25C
Polyethylene glycol 8,000
HR2-515 flake 500 g $122.00
HR2-535 50% w/v solution 200 ml $80.00
DNases ............. none detected
RNases ............. none detected
Proteases ......... none detected
Phosphatases ... none detected
Peroxides (as H2O2) 0.001%
Al ........................... 0.0005%
As ........................ 0.00001%
Ba .......................... 0.0005%
Bi ........................... 0.0005%
Ca ............................ 0.001%
Cd .......................... 0.0005%
Cl ............................. 0.005%
Co .......................... 0.0005%
Cr ........................... 0.0005%
Cu .......................... 0.0005%
Fe .......................... 0.0005%
K ................................ 0.02%
Li ............................ 0.0005%
Mg .......................... 0.0005%
Mn .......................... 0.0005%
Mo .......................... 0.0005%
Na .............................. 0.02%
Ni ........................... 0.0005%
Pb .......................... 0.0005%
SO
4 .......................... 0.005%
Sr ........................... 0.0005%
Zn .......................... 0.0005%
Synonyms: PEG 8,000 H(OCH2CH2)nOH Mr 7,000 - 9,000
CAS [25322-68-3] EC No 500-038-2
HR2-535: Measured pH Range: 6.6 - 9.5 at 25C
Refractive Index Range: 1.40206 - 1.40261 at 20C
Conductivity Range: 30.4 - 39.3 mS at 25C
Polyethylene glycol 10,000
HR2-607 50% w/v solution 200 ml $80.00
Synonyms: PEG 10,000 H(OCH2CH2)nOH Mr 8,500 - 11,500
CAS [25322-68-3] EC No 500-038-2
Measured pH Range: 6.4 - 8.6 at 25C
Refractive Index Range: 1.40165 - 1.40254 at 20C Conductivity Range: 39.4 - 48.3 mS at 25C
Polyethylene glycol 20,000
HR2-609 30% w/v solution 200 ml $80.00
Synonyms: PEG 20,000 H(OCH2CH2)nOH Average Mn ~16,000 - 24,000
CAS [25322-68-3] EC No 500-038-2
Measured pH Range: 4.6 - 10.2 at 25C
Refractive Index Range: 1.37438 - 1.37476 at 20C Conductivity Range: 45.3 - 64.5 mS at 25C
O
OH H
3
C
[ [
n
Polyethylene glycol 300
HR2-517 100% solution 200 ml $45.00
Synonyms: PEG 300 H(OCH2CH2)nOH Mr 285 - 315
CAS [25322-68-3] EC No 500-038-2 Density: 1.125 g/mL at 20C
Measured pH Range: 8.0 - 8.5 at 25C
Refractive Index Range: 1.46492 - 1.46535 at 20C
Conductivity Range: 7.7 - 8.1 mS at 25C
Peroxides (as H2O2) 0.001%
Al ........................... 0.0005%
As ........................ 0.00001%
Ba .......................... 0.0005%
Bi ........................... 0.0005%
Ca ............................ 0.001%
Cd .......................... 0.0005%
Cl ............................. 0.005%
Co .......................... 0.0005%
Cr ........................... 0.0005%
Cu .......................... 0.0005%
Fe .......................... 0.0005%
K .................................. 0.2%
Li ............................ 0.0005%
Mg .......................... 0.0005%
Mn .......................... 0.0005%
Mo .......................... 0.0005%
Na ................................ 0.2%
Ni ........................... 0.0005%
Pb .......................... 0.0005%
SO
4 .......................... 0.005%
Sr ........................... 0.0005%
Zn .......................... 0.0005%
O P T I M I Z E


- R E A G E N T S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 42
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P O L Y M E R S
Polyethylene glycol monomethyl ether 5,000
HR2-615 50% w/v solution 200 ml $85.00
Polyvinylpyrrolidone K 15
HR2-769 50% w/v solution 200 ml $45.00
Polypropylene glycol P 400
HR2-771 100% solution 200 ml $30.00
1,4-Dioxane
HR2-617 100% solution 100 ml $48.00
2-Propanol
HR2-619 100% solution 200 ml $46.00
DNases................none detected
RNases................none detected
Proteases............none detected
Phosphatases......none detected
Al .......................... 0.00005%
Ba ......................... 0.00001%
Bi .......................... 0.00001%
Ca ......................... 0.00005%
Cd ....................... 0.000005%
Co ....................... 0.000002%
Cr ........................ 0.000002%
Cu ....................... 0.000002%
Fe ......................... 0.00001%
K ........................... 0.00005%
Li ........................... 0.00001%
Mg ......................... 0.00001%
Mn ....................... 0.000002%
Mo ......................... 0.00001%
Na ......................... 0.00005%
Ni ........................ 0.000002%
Pb ......................... 0.00001%
Sr .......................... 0.00001%
Zn ......................... 0.00001%
Al .......................... 0.00005%
Ba ......................... 0.00001%
Bi .......................... 0.00001%
Ca ......................... 0.00005%
Cd ....................... 0.000005%
Co ....................... 0.000002%
Cr ........................ 0.000002%
Cu ....................... 0.000002%
Fe ......................... 0.00001%
K ........................... 0.00005%
Li ........................... 0.00001%
Mg ......................... 0.00001%
Mn ....................... 0.000002%
Mo ......................... 0.00001%
Na ......................... 0.00005%
Ni ........................ 0.000002%
Pb ......................... 0.00001%
Sr .......................... 0.00001%
Zn ......................... 0.00001%
O R G A N I C S ( V O L A T I L E )
Ethylene glycol
HR2-621 100% solution 100 ml $34.00
A ........................ 0.00005%
Ba ...................... 0.00001%
Bi ....................... 0.00001%
Ca ...................... 0.00005%
Cd .................... 0.000005%
Cl ........................... 0.001%
Co .................... 0.000002%
Cr ..................... 0.000002%
Cu .................... 0.000002%
Fe ...................... 0.00005%
K ........................ 0.00005%
Li ........................ 0.00001%
Mg ...................... 0.00001%
Mn .................... 0.000005%
Mo ...................... 0.00001%
Na ........................ 0.0001%
Ni ..................... 0.000002%
Pb ...................... 0.00001%
SO4 ........................ 0.001%
Sr ....................... 0.00001%
Zn ...................... 0.00002%
Glycerol
HR2-623 100% solution 100 ml $61.00
DNases ........... None detected
RNases ........... None detected
Proteases ....... None detected
Phosphatases . None detected
Ag ........................ 0.0005%
Al ......................... 0.0001%
As ...................... 0.00001%
Ba ........................ 0.0001%
Bi ......................... 0.0001%
Ca ........................ 0.0005%
Cd ........................ 0.0001%
Cl ......................... 0.0001%
Co ........................ 0.0001%
Cr ......................... 0.0001%
Cu ....................... 0.0001%
Fe ........................ 0.0001%
K ............................ 0.002%
Li .......................... 0.0001%
Mg ........................ 0.0001%
Mn ........................ 0.0001%
Mo ........................ 0.0001%
NH4
+
.................... 0.0005%
Na .......................... 0.002%
Ni ......................... 0.0001%
Pb ........................ 0.0001%
SO4 ........................ 0.001%
Sr ......................... 0.0001%
Tl .......................... 0.0005%
Zn ........................ 0.0001%
1,6-Hexanediol
HR2-625 6.0 M (71% w/v) solution 200 ml $52.00
(+/-)-2-Methyl-2,4-pentandiol
HR2-627 100% solution 200 ml $62.00
O R G A N I C S ( N O N - V O L A T I L E )
Synonyms: Methoxypolyethylene glycol 5,000 or
mono-Methyl polyethylene glycol 5,000 or PEG MME 5,000
CH3O(CH2CH2O)nH CAS [9004-74-4] EC No 215-801-2
Purity > 95.0% Measured pH Range: 7.7 - 8.9 at 25C
Refractive Index Range: 1.40199 - 1.40236 at 20C Conductivity Range: 19.8 - 25.7 mS at 25C
Synonyms: 1,2-Ethanediol
C2H6O2 or HOCH2CH2OH Mr 62.07
CAS [107-21-1] EC No 203-473-3
Density: 1.113 g/mL at 25C (lit.) Purity > 99.5%
Measured pH Range: 5.5 - 7.8 at 25C Refractive Index Range: 1.43174 - 1.43207 at 20C
Conductivity Range: 0.1 - 0.8 mS at 25C
Synonyms: None
(C3H6O)n CAS [25322-69-4]
EC No 500-039-8 Density: 1.01 g/mL at 20C
Measured pH Range: 3.8 - 8.5 at 25C
Refractive Index Range: 1.44501 - 1.44827 at 20C Conductivity Range: 0.0 - 0.1 mS at 25C
Synonyms: 1,2,3-Propanetriol or Glycerin
HOCH2CH(OH)CH2OH or C3H8O3 Mr 92.09
CAS [56-81-5] EC No 200-289-5 Density: 1.25 g/mL (lit.)
Purity > 99.5% Measured pH Range: 6.9 - 7.4 at 25C
Refractive Index Range: 1.47220 - 1.47412 at 20C Conductivity Range: 0.0 mS at 25C
Synonyms: Polyvidone or Povidone or PVP or
Poly(1-vinyl-2-pyrrolidinone) homopolymer
(C6H9NO)n Average M
r
~10,000 CAS [9003-39-8] EC No 201-800-4
Measured pH Range: 4.2 - 5.4 at 25C
Refractive Index Range: 1.42196 - 1.42234 at 20C Conductivity Range: 476 - 812 mS at 25C
Synonyms: Diethylene oxide or Dioxane
C4H8O2 M
r
88.11
CAS [123-91-1]
EC No 204-661-8 Purity > 99.5%
Synonyms: Hexamethylene glycol
HO(CH2)6OH or C6H14O2 Mr
118.18
CAS [629-11-8] EC No 211-074-0 Purity > 97.0%
Measured pH Range: 5.3 - 8.2 at 25C
Refractive Index Range: 1.42351 - 1.42386 at 20C Conductivity Range: 0.1 - 1.5 mS at 25C
Synonyms: MPD or Hexylene glycol
CH3CH(OH)CH2C(CH3)2OH or C6H14O2
Mr
118.18 CAS [107-41-5] EC No 203-489-0
Purity > 99.0% Density: 0.925 g/mL at 25 C (lit.)
Refractive Index Range: 1.42755 - 1.42787 at 20C
Conductivity Range: 0.0 - 0.3 mS at 25C
Synonyms: sec-Propyl alcohol or Isopropanol or Isopropyl alcohol
(CH3)2CHOH or C3H8O Mr 60.10 CAS [67-63-0]
EC No 200-661-7 Purity > 99.5% Density: 0.785 g/mL at 25C (lit.)
Refractive Index Range: 1.37694 - 1.37748 at 20C
O
OH H
[ [
n
CH
3
O
OH H
3
C
[ [
n
HO
OH
OH
H
3
C CH
3
O
O
CH
3
CHCH
2
C
OH OH
CH
3
CH
3
Protein crystals with a wheat-sheaf habit.
Jennifer Wingard, University of Maryland, USA.
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S A L T S
Ammonium acetate
HR2-565 1.0 M solution 100 ml $70.00
HR2-799 8.0 M solution 200 ml $98.00
Al ......................... 0.0005%
As ...................... 0.00001%
Ba ........................ 0.0005%
Bi ......................... 0.0005%
Ca .......................... 0.001%
Cd ........................ 0.0005%
Cl ......................... 0.0005%
Co ........................ 0.0005%
Cr ......................... 0.0005%
Cu ........................ 0.0005%
Fe ........................ 0.0002%
K ............................ 0.005%
Li .......................... 0.0005%
Mg ........................ 0.0005%
Mn ........................ 0.0005%
Mo ........................ 0.0005%
Na .......................... 0.005%
Ni ......................... 0.0005%
NO3........................ 0.001%
Pb ........................ 0.0005%
SO4 ........................ 0.001%
Sr ......................... 0.0005%
Zn ........................ 0.0005%
Ammonium chloride
HR2-691 5.0 M solution 200 ml $65.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0002%
Fe ........................... 0.0002%
K ............................... 0.005%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Na ............................. 0.005%
Ni ............................ 0.0001%
NO
3......................... 0.0005%
Pb ........................... 0.0001%
PO
4 ......................... 0.0002%
SO4 ........................... 0.002%
Sr ............................ 0.0005%
Zn ........................... 0.0002%
Ammonium phosphate monobasic
HR2-555 2.5 M solution 200 ml $68.00
Al ............................ 0.0005%
As ......................... 0.00005%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.005%
Cd ........................... 0.0005%
Cl .............................. 0.005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ............................. 0.002%
K ............................... 0.005%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Na ............................. 0.005%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO4 ........................... 0.005%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
Ammonium fluoride
HR2-689 10.0 M solution 200 ml $71.00
Ammonium formate
HR2-659 10.0 M solution 200 ml $134.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl .............................. 0.005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Na ............................. 0.005%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO
4 ........................... 0.005%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
Ammonium citrate dibasic
HR2-685 2.5 M solution 200 ml $95.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl ............................ 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Na ............................. 0.005%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
PO
4 ......................... 0.0005%
SO4 ........................... 0.005%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
Ammonium phosphate dibasic
HR2-629 3.5 M solution 200 ml $75.00
Al ........................... 0.0005%
As ........................ 0.00001%
Ba .......................... 0.0005%
Bi ........................... 0.0005%
Ca ............................ 0.001%
Cd .......................... 0.0005%
Cl ........................... 0.0005%
Co .......................... 0.0005%
Cr ........................... 0.0005%
Cu .......................... 0.0005%
Fe .......................... 0.0005%
K .............................. 0.005%
Li ............................ 0.0005%
Mg .......................... 0.0005%
Mn .......................... 0.0005%
Mo .......................... 0.0005%
Na ............................ 0.005%
Ni ........................... 0.0005%
NO3.......................... 0.001%
Pb .......................... 0.0005%
SO
4 .......................... 0.002%
Sr ........................... 0.0005%
Zn .......................... 0.0005%
Ammonium nitrate
HR2-665 10.0 M solution 200 ml $89.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl ............................ 0.0003%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0002%
K ............................... 0.005%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Na ............................. 0.005%
Ni ............................ 0.0005%
NO2......................... 0.0005%
Pb ........................... 0.0005%
PO
4 ......................... 0.0005%
SO4 ........................... 0.002%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
Ammonium citrate tribasic pH 7.0
HR2-759 pH 7.0 - 2.5 M solution 200 ml $95.00
Synonyms: None C2H7NO2 or CH3CO2NH4
Mr 77.08 CAS [631-61-8] EC No 211-162-9 Purity > 99.0%
HR2-565: Measured pH Range: 6.8 - 7.1 at 25C
Refractive Index Range: 1.34382 - 1.34410 at 20C
Conductivity Range: 62.0 - 70.5 mS at 25C
HR2-799: Measured pH Range: 7.6 - 7.9 at 25C
Refractive Index Range: 1.40811 - 1.40885 at 20C
Conductivity Range: 68.9 - 80.0 mS at 25C
Synonyms: Formic acid ammonium salt
HCOONH4 or CH5NO2 Mr 63.06
CAS [540-69-2] EC No 208-753-9 Purity > 99.0%
Measured pH Range: 7.0 - 7.3 at 25C
Refractive Index Range: 1.40348 - 1.40398 at 20C
Conductivity Range: 579.6 - 706.0 mS at 25C
Synonyms: None NH4NO3 Mr 80.04
CAS [6484-52-2] EC No 229-347-8 Purity > 99.5%
Measured pH Range: 3.8 - 5.6 at 25C
Refractive Index Range: 1.41941 - 1.41957 at 20C
Conductivity Range: 942 - 1036 mS at 25C
Synonyms: Ammonium hydrogenphosphate or
di-Ammonium hydrogen phosphate (sec) or
Diammonium hydrogen phosphate
(NH4)2HPO4 or H9N2O4P Mr 132.06
CAS [7783-28-0] EC No 231-987-8 Purity > 99.0%
Measured pH Range: 7.8 - 8.1 at 25C Refractive Index Range: 1.40135 - 1.40362 at 20C
Conductivity Range: 87.4 - 105.7 mS at 25C
Synonyms: Ammonium dihydrogen phosphate or
Mono-ammonium phosphate or prim-Ammonium phosphate
NH4H2PO4 Mr 115.03 CAS [7722-76-1] EC No 231-764-5
Purity > 99.5% Measured pH Range: 3.5 - 3.8 at 25C
Refractive Index Range: 1.36710 - 1.36745 at 20C
Conductivity Range: 81.9 - 95.5 mS at 25C
Synonyms: Salmiac NH4Cl Mr 53.49
CAS [12125-02-9] EC No 235-186-4 Purity > 99.5%
Measured pH Range: 3.9 - 4.3 at 25C Refractive Index Range: 1.38063 - 1.38075 at 20C
Conductivity Range: 515.2 - 588.0 mS at 25C
Synonyms: Citric acid ammonium salt or
Diammonium hydrogen citrate or Di-Ammnonium citrate
C6H14N2O7 or (NH4)2C6H6O7 or HOC(CO2H)(CH2CO2NH4)2
Mr 226.19 CAS [3012-65-5] EC No 221-146-3 Purity > 99.0%
Measured pH Range: 4.7 - 4.8 at 25C
Refractive Index: 1.42003 at 20C
Conductivity Range: 76.8 - 81.9 mS at 25C
Synonyms: Citric acid triammonium salt
HOC(CO2NH4)(CH2CO2NH4)2 or C6H17N3O7
Mr 243.22 CAS [3458-72-8] EC No 222-394-5 Purity > 97.0%
HR2-759 titrated to pH 7.0 at 25C using Hydrochloric acid (HR2-581)
Refractive Index Range: 1.43010 - 1.43183 at 20C
Conductivity Range: 90.4 - 100.8 mS at 25C
Synonyms: None NH4F Mr 37.04 CAS [12125-01-8] EC No 235-185-9
Purity > 98.0% Measured pH Range: 7.4 - 8.0 at 25C Refractive Index: 1.42003 at 20C
Conductivity Range: 350.8 - 428.8 mS at 25C
O
H
3
C ONH
4
N
O O
Ag ........................... 0.0005%
Al ............................ 0.0005%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.005%
Cd ........................... 0.0005%
Cl ............................ 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Na ............................. 0.005%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SiF
6............................... 0.1%
SO4 ........................... 0.005%
Sr ............................ 0.0005%
Ti ............................. 0.0005%
Zn ........................... 0.0005%
O P T I M I Z E


- R E A G E N T S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 44
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Calcium chloride dihydrate
HR2-557 2.0 M solution 100 ml $74.00
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Cd ........................... 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Li ............................. 0.0005%
Mg ............................. 0.005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
N ............................... 0.002%
Na ............................... 0.01%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO4 ........................... 0.005%
Sr ................................ 0.01%
Zn ........................... 0.0005%
Cesium chloride
HR2-719 1.0 M solution 100 ml $128.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.005%
Cd ........................... 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
N ............................... 0.001%
Na ............................. 0.005%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO4 ........................... 0.002%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
Cobalt(II) chloride hexahydrate
HR2-713 1.0 M solution 100 ml $64.00
Iron(III) chloride hexahydrate
HR2-717 1.0 M solution 100 ml $58.00
As ......................... 0.0005%
Ca ........................... 0.005%
Cd ........................... 0.001%
Cl ............................ 0.002%
Co ........................... 0.005%
Cr .............................. 0.01%
Cu ........................... 0.002%
Fe2
+
........................ 0.002%
K ............................. 0.005%
Mg ........................... 0.001%
Mn ............................... 0.1%
N ............................. 0.001%
Na ............................. 0.01%
Ni ............................ 0.005%
NO3........................... 0.01%
Pb ........................... 0.002%
PO4 ........................... 0.01%
SO4 ......................... 0.005%
Zn ........................... 0.002%
Hexadecyltrimethylammonium bromide
HR2-711 0.05 M solution 200 ml $61.00
Al .......................... 0.0005%
As ....................... 0.00005%
Ba ......................... 0.0005%
Bi .......................... 0.0005%
Ca ........................... 0.001%
Cd ......................... 0.0005%
Co ......................... 0.0005%
Cr .......................... 0.0005%
Cu ......................... 0.0005%
Fe ......................... 0.0005%
K ............................. 0.005%
Li ........................... 0.0005%
Mg ......................... 0.0005%
Mn ......................... 0.0005%
Mo ......................... 0.0005%
Na ........................... 0.005%
Ni .......................... 0.0005%
Pb ......................... 0.0005%
SO4 ......................... 0.005%
Sr .......................... 0.0005%
Zn ......................... 0.0005%
Cadmium sulfate hydrate
HR2-721 1.0 M solution 100 ml $88.00
As ......................... 0.00001%
Ca ............................. 0.005%
Cl .............................. 0.001%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
N ............................. 0.0005%
Na ............................. 0.005%
Ni ............................ 0.0005%
Pb ............................. 0.002%
Zn ............................. 0.005%
Calcium acetate hydrate
HR2-567 1.0 M solution 100 ml $68.00
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Cd ........................... 0.0005%
Cl .............................. 0.005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ................................. 0.01%
Li .............................. 00005%
Mg ................................. 0.1%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Na ............................... 0.01%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO4 ............................. 0.01%
Sr ................................ 0.02%
Zn ........................... 0.0005%
S A L T S
Ammonium sulfate
HR2-541 3.5 M solution 200 ml $68.00
Al ............................ 0.0005%
As ......................... 0.00002%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0001%
Cl ............................ 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0002%
Fe ........................... 0.0002%
K ............................... 0.005%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Na ............................. 0.005%
Ni ............................ 0.0005%
NO3........................... 0.001%
Pb ........................... 0.0002%
PO4 ......................... 0.0005%
Sr ............................ 0.0005%
Zn ........................... 0.0001%
Ammonium tartrate dibasic
HR2-679 2.0 M solution 200 ml $75.00
HR2-767 pH 7.0 - 1.6 M solution 200 ml $94.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl .............................. 0.005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Na ............................. 0.005%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO4 ........................... 0.005%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
Cadmium chloride hydrate
HR2-715 1.0 M solution 100 ml $98.00
Synonyms: Ammonium sulphate
(NH4)2SO4 or H8N2O4S Mr 132.14
CAS [7783-20-2] EC No 231-984-1 Purity > 99.5%
Measured pH Range: 5.0 - 5.2 at 25C
Refractive Index Range: 1.39131 - 1.39166 at 20C
Conductivity Range: 313.2 - 424.2 mS at 25C
Synonyms: None CaCl2 2H2O Mr 147.01
CAS [10035-04-8] EC No 233-1408 Purity > 99.5%
Measured pH Range: 6.1 - 7.9 at 25C Refractive Index Range: 1.38108 - 1.38195 at 20C
Conductivity Range: 170.0 - 196.9 mS at 25C
Synonyms: None CsCl Mr 168.36 CAS [7647-17-8] EC No 231-600-2
Purity > 99.0% Measured pH Range: 6.0 - 6.5 at 25C
Refractive Index: 1.34591 at 20C
Conductivity Range: 106.5 - 112.9 mS at 25C
Synonyms: Cobaltous chloride hexahydrate Cl2Co2 6H2O Mr 237.93
CAS [7791-13-1] EC No 231-589-4 Purity > 98.0%
Measured pH Range: 2.1 - 3.6 at 25C
Conductivity Range: 103.4 - 114.8 mS at 25C
Synonyms: CTAB or cetrimide or cetrimonium bromide or
cetyltrimethylammonium bromide or
palmityltrimethylammonium bromide
C19H42BrN or CH3(CH2)15N(Br)(CH3)3 Mr 364.45
CAS [57-09-0] EC No 200-311-3 Purity > 99.0%
Measured pH Range: 5.3 - 5.9 at 25C
Refractive Index: 1.33573 at 20C
Conductivity Range: 1109 - 1344 mS at 25C
Synonyms: Ferric chloride hexahydrate
FeCl3 6H2O or Cl3Fe 6H2O Mr 270.30 CAS [10025-77-1] EC No 231-729-4
Measured pH Range: 0.8 - 1.0 at 25C Refractive Index: 1.37469 at 20C
Conductivity Range: 90.0 - 94.8 mS at 25C
Synonyms: L-(+)-Tartaric acid diammonium salt or
Diammonium tartrate (NH4)2C4H4O6 or C4H12N2O6 Mr 184.15
CAS [3164-29-2] EC No 221-618-9 Purity > 99.5%
HR2-679: Measured pH Range: 6.4 - 6.8 at 25C
Refractive Index Range: 1.39234 - 1.39254 at 20C
Conductivity Range: 118.2 - 134.4 mS at 25C
HR2-767: Titrated to pH 7.0 at 25C using Sodium hydroxide (HR2-583)
Refractive Index Range: 1.38139 - 1.38147 at 20C
Conductivity Range: 115.7 - 128.5 mS at 25C
Synonyms: None CdCl2 xH2O Mr 183.32 (anhyd.) CAS [654054-66-7] EC No 233-296-7
Purity 98.0% Density: 3.327 g/mL at 25C (lit.) Measured pH Range: 3.3 - 5.0 at 25C
Refractive Index Range: 1.35455 - 1.35477 at 20C Conductivity Range: 26.9 - 28.9 mS at 25C
Synonyms: Cadmium sulfate octahydrate 3CdSO4 8H2O Mr
769.52
CAS [7790-84-3] EC No 233-331-6
Purity > 99.0% (calc. based on CdSO4 8/3 H2O, KT)
Measured pH Range: 3.5 - 4.4 at 25C
Refractive Index: 1.40310 at 20C
Conductivity Range: 40.5 - 45.3 mS at 25C
Synonyms: None (CH3COO)2Ca xH2O C4H6CaO4 xH2O
Mr 158.17 (anhyd.) CAS [114460-21-8] EC No 200-540-9
Purity > 99.0% Measured pH Range: 7.6 - 7.9 at 25C
Refractive Index Range: 1.35719 - 1.35722 at 20C
Conductivity Range: 34.1 - 38.1 mS at 25C
Co Cl
Cl
O
O
-
S
O
-
O
Cd
++
45
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Lithium acetate dihydrate
Al ............................ 0.0005%
Ca ............................. 0.005%
Cl .................................. 0.5%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Mg ............................. 0.001%
NH
4
+
........................... 0.05%
Na ............................. 0.005%
P ............................. 0.0005%
Pb ............................. 0.001%
SO4 ............................. 0.05%
Zn ........................... 0.0005%
HR2-669 5.0 M solution 200 ml $93.00
Lithium chloride
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ............................. 0.001%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
N ............................... 0.001%
Na ............................. 0.005%
Ni .............................. 0.001%
Pb ........................... 0.0005%
SO4 ........................... 0.005%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
HR2-631 10.0 M solution 200 ml $124.00
Lithium citrate tribasic tetrahydrate
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl .............................. 0.005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Na ............................. 0.005%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO4 ........................... 0.005%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
HR2-681 1.5 M solution 200 ml $98.00
Lithium nitrate
Al .............................. 0.001%
As ......................... 0.00001%
Ba ............................. 0.001%
Bi ............................ 0.0005%
Ca ............................... 0.01%
Cd ........................... 0.0005%
Cl .............................. 0.005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ................................. 0.02%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Na ............................... 0.02%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO
4 ............................. 0.01%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
HR2-697 8.0 M solution 200 ml $85.00
HR2-545 2.0 M solution 200 ml $98.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl .............................. 0.005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Na ............................. 0.005%
Ni ............................ 0.0005%
NO3........................... 0.001%
Pb ........................... 0.0005%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
S A L T S
Lithium sulfate monohydrate
Magnesium acetate tetrahydrate
HR2-561 1.0 M solution 100 ml $81.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi .............................. 0.001%
Ca ............................. 0.002%
Cd ........................... 0.0005%
Cl .............................. 0.005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Li ............................. 0.0005%
Mn ............................. 0.002%
Mo ........................... 0.0005%
Na ............................. 0.005%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO4 ............................. 0.02%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
Synonyms: Acetic acid lithium salt CH3COOLi 2H2O
Mr 102.02 CAS [6108-17-4] EC No 208-914-3
Measured pH Range: 8.3 - 9.2 at 25C
Refractive Index: 1.38124 at 20C
Conductivity Range: 24.7 - 26.5 mS at 25C
Synonyms: Lithium chloride anhydrous
LiCl Mr 42.39 CAS [7447-41-8] EC No 231-212-3 Purity > 99.0%
Measured pH Range: 5.7 - 6.6 at 25C
Refractive Index: 1.41030 at 20C
Conductivity Range: 107.4 - 132.9 mS at 25C
Synonyms: Acetic acid magnesium salt
Mg(CH
3
COO)2Mg 4H2O or C4H6MgO4 4H2O
Mr 214.45 CAS [16674-78-5] EC No 205-554-9
Purity > 99.0% Measured pH Range: 8.1 - 8.4 at 25C
Refractive Index Range: 1.35767 - 1.35799 at 20C
Conductivity Range: 32.4 - 36.8 mS at 25C
Synonyms: None MgCl2 6H2O Mr 203.30
CAS [7791-18-6] EC No 232-094-6 Purity > 99.0%
HR2-559: Measured pH Range: 5.1 - 6.0 at 25C
Refractive Index Range: 1.37544 - 1.37603 at 20C
Conductivity Range: 136.2 - 160.2 mS at 25C
HR2-803: Measured pH Range: 3.7 - 5.7 at 25C Refractive Index: 1.43079 at 20C
Conductivity Range: 60.0 - 84.5 mS at 25C
Synonyms: Diformic acid magnesium salt C2H2O4Mg 2H2O
Mr 150.38 CAS [557-39-1] EC No 209-173-9 Purity 98.5%
Measured pH Range: 7.6 - 7.8 at 25C
Refractive Index Range: 1.35214 - 1.35241 at 20C
Conductivity Range: 45.6 - 50.3 mS at 25C
Synonyms: Magnesium nitrate
Mg(NO3)2 6H2O MgN2O6 6H2O Mr 256.41
CAS [13446-18-9] EC No 233-826-7 Purity > 99.0%
Measured pH Range: 2.7 - 3.8 at 25C
Refractive Index Range: 1.39348 - 1.39373 at 20C
Conductivity Range: 112.4 - 136.1 mS at 25C
Synonyms: Epsom salts MgSO4 7H2O Mr 246.47
CAS [10034-99-8] EC No 231-298-2 Purity > 99.5%
Measured pH: 5.6 at 25C
Conductivity Range: 51.8 mS at 25C
Synonyms: Citric acid trilithium salt or Trilithium citrate tetrahydrate
HOC(COOLi)(CH2COOLi)2 4H2O or C6H5Li3O7 4H2O
Mr 281.99 CAS [6080-58-6] EC No 213-045-8 Purity > 99.5%
Measured pH Range: 8.4 - 9.3 at 25C
Refractive Index Range: 1.38910 - 1.38970 at 20C
Conductivity Range: 30.9 - 32.5 mS at 25C
Synonyms: None LiNO3 Mr
68.95
CAS [7790-69-4] EC No 232-218-9 Purity > 99.0%
Measured pH Range: 7.0 - 7.8 at 25C
Refractive Index: 1.39391 at 20C
Conductivity Range: 139.2 - 151.5 mS at 25C
Synonyms: Lithium sulfate Li2SO4 H2O or Li2O4S H2O
Mr 127.96 CAS [10102-25-7] EC No 233-820-4 Purity > 99.0%
Measured pH Range: 3.0 - 4.3 at 25C
Refractive Index Range: 1.36520 - 1.36553 at 20C
Conductivity Range: 76.4 - 86.5 mS at 25C
Magnesium chloride hexahydrate
HR2-559 2.0 M solution 100 ml $76.00
HR2-803 5.0 M solution 200 ml $98.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.005%
Cd ........................... 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ................................... 0.2%
Li ............................. 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
N ............................. 0.0002%
Na ................................. 0.3%
Ni ............................ 0.0005%
PO4 ......................... 0.0005%
Pb ........................... 0.0005%
SO4 ........................... 0.005%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
Magnesium sulfate heptahydrate
HR2-821 2.0 M solution 200 ml $102.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl ............................ 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0001%
K ............................... 0.001%
Li ............................. 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
N ............................... 0.002%
Na ............................. 0.001%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
Magnesium nitrate hexahydrate
HR2-657 3.0 M solution 200 ml $83.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ............................. 0.001%
Bi .............................. 0.001%
Ca ................................. 0.5%
Cd ........................... 0.0005%
Cl .............................. 0.005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ................................. 0.01%
Li ............................. 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Na ............................... 0.01%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO
4
........................... 0.005%
Sr .............................. 0.001%
Zn ........................... 0.0005%
Magnesium formate dihydrate
HR2-537 1.0 M solution 200 ml $82.00
O P T I M I Z E


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Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 46
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S A L T S
Magnesium sulfate hydrate
HR2-633 2.5 M solution 200 ml $83.00
Ca ............................... 0.02%
Cd ............................. 0.005%
Cl ................................ 0.01%
Co ............................. 0.005%
Cu ............................. 0.005%
Fe ............................. 0.005%
K ................................. 0.05%
Na ............................... 0.01%
Ni .............................. 0.005%
Pb ............................. 0.005%
Zn ............................. 0.005%
Nickel(II) chloride hexahydrate
HR2-687 4.0 M solution 200 ml $69.00
Ca ............................. 0.005%
Cd ............................. 0.005%
Co ............................... 0.05%
Cu ............................. 0.005%
Fe ............................. 0.005%
K ................................. 0.01%
Pb ............................. 0.005%
Zn ............................. 0.005%
Potassium acetate
DNases................none detected
RNases................none detected
Proteases............none detected
Phosphatases......none detected
Pb .... 5ppm (parts per million)
HR2-671 5.0 M solution 200 ml $85.00
Potassium citrate tribasic monohydrate
HR2-683 2.5 M solution 200 ml $74.00
DL-Malic acid pH 7.0
HR2-761 pH 7.0 - 3.0 M solution 200 ml $62.00
Potassium fluoride
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl .............................. 0.005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
HF ............................. 0.001%
KOH ............................ 0.01%
K2SiF6 ...................... 0.003%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Na ................................. 0.2%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO4 ........................... 0.005%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
HR2-647 6.0 M solution 100 ml $68.00
Potassium formate
Ca ............................. 0.005%
Cd ............................. 0.005%
Cl .............................. 0.005%
Co ............................. 0.005%
Cu ............................. 0.005%
Fe ............................. 0.005%
Na ................................. 0.5%
Ni .............................. 0.005%
Pb ............................. 0.005%
SO4 ........................... 0.005%
Zn ............................. 0.005%
HR2-667 14.0 M solution 200 ml $118.00
Potassium phosphate dibasic
HR2-635 4.0 M solution 200 ml $74.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl .............................. 0.005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Na ................................. 0.5%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO
4 ........................... 0.005%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
Synonyms: Magnesium sulphate hydrate MgSO4 xH2O
Mr 120.37 (anhyd.) CAS [22189-08-8] EC No 231-298-2 Purity > 99.0%
Measured pH Range: 6.2 - 8.2 at 25C
Refractive Index: 1.37187 at 20C
Conductivity Range: 49.2 - 59.5 mS at 25C
Synonyms: None KCl Mr 74.55
CAS [7447-40-7] EC No 231-211-8 Purity > 99.5%
Measured pH Range: 5.4 - 8.1 at 25C Refractive Index Range: 1.36868 - 1.36882 at 20C
Conductivity Range: 391.0 - 449.6 mS at 25C
Synonyms: Citric acid tripotassium salt or tri-potassium citrate
HOC(COOK)(CH2COOK)2 H2O or C6H5K3O7 H2O
Mr 324.41 CAS [6100-05-6] EC No 231-905-0 Purity > 98.0%
Measured pH Range: 8.2 - 9.9 at 25C
Refractive Index: 1.42503 at 20C
Conductivity Range: 80.6 - 91.7 mS at 25C
Synonyms: None KF or FK Mr 58.10
CAS [7789-23-3] EC No 232-151-5 Purity > 99.5%
Measured pH Range: 8.0 - 10.0 at 25C
Refractive Index: 1.35344 at 20C
Conductivity Range: 346.6 - 416.2 mS at 25C
Synonyms: Formic acid potassium salt HCOOK or CHKO2
Mr 84.12 CAS [590-29-4] EC No 209-677-9 Purity > 99.0%
Measured pH Range: 9.5 - 10.8 at 25C
Refractive Index: 1.41516 at 20C
Conductivity Range: 77.9 - 99.4 mS at 25C
Synonyms: Dipotassium hydrogenphosphate or
Dipotassium phosphate or sec.-Potassium phosphate
K2HPO4 or HK2O4P Mr 174.18 CAS [7758-11-4] EC No 231-834-5
Measured pH Range: 9.6 - 9.8 at 25C
Refractive Index Range: 1.40781 - 1.40791 at 20C
Conductivity Range: 126.9 - 148.5 mS at 25C
Synonyms: Nickel chloride NiCl2 6H2O Mr 237.69
CAS [7791-20-0] EC No 231-743-0 Purity > 98.0%
Measured pH Range: 3.1 - 3.8 at 25C
Refractive Index Range: 1.43878 - 1.43890 at 20C
Conductivity Range: 82.2 - 104.6 mS at 25C
Synonyms: ()-2-Hydroxysuccinic acid or DL-Hydroxybutanedioic acid
HO2CCH2CH(OH)CO2H or C4H6O5
Mr 134.09 CAS [6915-15-7] EC No 230-022-8 Purity > 99.0%
Titrated to pH 7.0 at 25C using Sodium hydroxide (HR2-583)
Refractive Index Range: 1.40605 - 1.40638 at 20C
Conductivity Range: 43.0 - 52.1 mS at 25C
Synonyms: None KBr or BrK Mr 119.00
CAS [7758-02-3] EC No 231-830-3 Purity > 99.5%
Measured pH Range: 8.2 - 8.6 at 25C
Refractive Index Range: 1.38454 - 1.38686 at 20C
Conductivity Range: 384.6 - 438.8 mS at 25C
Synonyms: K(acac) C2H3KO2 or CH3COOK Mr 98.14
CAS [127-08-2] EC No 204-822-2 Purity > 99.0%
Measured pH Range: 7.9 - 8.9 at 25C
Refractive Index Range: N/A
Conductivity Range: 132.3 - 146.7 mS at 25C
Potassium bromide
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
BrO
3.......................... 0.001%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl .................................. 0.1%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
I................................. 0.001%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
N ............................... 0.001%
Na ............................... 0.02%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO4 ........................... 0.005%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
HR2-779 4.0 M solution 100 ml $74.00
Potassium chloride
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ............................. 0.001%
Bi ............................ 0.0005%
Br ................................ 0.05%
Ca ............................. 0.005%
Cd ........................... 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0002%
I................................. 0.002%
Li ............................. 0.0005%
Mg ............................. 0.001%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
N ............................... 0.001%
Na ............................... 0.02%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
PO
4 ......................... 0.0005%
SO4 ........................... 0.003%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
HR2-649 4.0 M solution 200 ml $80.00
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S A L T S
Potassium nitrate
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.005%
Cd ........................... 0.0005%
Cl ................................ 0.01%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0002%
IO
3 .......................... 0.0005%
Li ............................. 0.0005%
Mg ............................. 0.005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
NH4
+
......................... 0.001%
Na ............................. 0.005%
Ni ............................ 0.0005%
NO2........................... 0.001%
Pb ........................... 0.0005%
PO
4 ......................... 0.0005%
SO4 ........................... 0.002%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
HR2-663 3.0 M solution 200 ml $79.00
Potassium sulfate
HR2-675 0.5 M solution 200 ml $65.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.005%
Cd ........................... 0.0005%
Cl ............................ 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
Li ............................. 0.0005%
Mg ............................. 0.002%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
N ............................. 0.0005%
Na ............................. 0.005%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
PO4 ........................... 0.001%
Sr .............................. 0.005%
Zn ........................... 0.0005%
Potassium thiocyanate
HR2-695 8.0 M solution 200 ml $105.00
Al .............................. 0.001%
Ca ............................. 0.001%
Cl .............................. 0.005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
Mg ........................... 0.0005%
NH
4
+
......................... 0.003%
Na ............................. 0.005%
Pb ............................. 0.001%
SO4 ........................... 0.005%
Zn ........................... 0.0005%
Potassium sodium tartrate tetrahydrate
HR2-539 1.5 M solution 200 ml $91.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl ............................ 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
N ............................... 0.002%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
PO4 ........................... 0.001%
SO4 ........................... 0.005%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
Sodium acetate trihydrate
Al ............................ 0.0005%
As ......................... 0.00001%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl ............................ 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
PO
4 ......................... 0.0005%
SO4 ........................... 0.002%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
HR2-543 3.0 M solution 200 ml $72.00
HR2-763 pH 7.0 - 4.0 M solution 200 ml $65.00
Sodium bromide
HR2-699 5.0 M solution 200 ml $41.00
Ca ............................. 0.005%
Cd ............................. 0.005%
Cl .................................. 0.1%
Co ............................. 0.005%
Cu ............................. 0.005%
Fe ............................. 0.005%
K ................................... 0.2%
Ni .............................. 0.005%
Pb ............................. 0.005%
SO4 ........................... 0.005%
Zn ............................. 0.005%
L-Proline
HR2-775 1.0 M solution 100 ml $60.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl ................................ 0.01%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Na ............................. 0.005%
NH4
+
........................... .002%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO4 ............................. 0.01%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
Sodium chloride
HR2-637 5.0 M solution 200 ml $33.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Br .............................. 0.005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0001%
I................................. 0.001%
K ............................... 0.005%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
N ............................... 0.001%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
PO4 ......................... 0.0005%
SO4 ............................. 0.01%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
Potassium phosphate monobasic
HR2-553 1.5 M solution 200 ml $69.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl ............................ 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
N ............................... 0.001%
Na ............................. 0.005%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO4 ........................... 0.003%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
Synonyms: Monopotassium phosphate or
Potassium dihydrogen phosphate or prim.-Potassium phosphate
H2KO4P or KH2PO4 Mr 136.09 CAS [7778-77-0]
EC No 231-913-4 Purity > 99.5%
pKa
1
= 2.15 at 25C
pKa
2
= 6.82 at 25C
pKa
3
= 12.38 at 25C
Measured pH Range: 3.8 - 4.3 at 25C
Refractive Index Range: 1.35379 - 1.35414 at 20C
Conductivity Range: 67.7 - 78.1 mS at 25C
Synonyms: Potassium rhodanide KSCN or CKNS
Mr 97.18 CAS [333-20-0] EC No 206-370-1 Purity > 99.0%
Measured pH Range: 7.2 at 25C
Refractive Index Range: 1.45988 at 20C
Conductivity Range: 767.6 mS at 25C
Synonyms: (S)-pyrrolidine-2-carboxylic acid C5H9NO2
Mr 115.13 CAS [147-85-3] EC No 205-702-2 Purity > 99.5%
Measured pH Range: 6.0 - 7.0 at 25C
Refractive Index Range: 1.35260 at 20C
Conductivity Range: 5.4 - 9.1 mS at 25C
Synonyms: None KNO3 Mr 101.10
CAS [7757-79-1] EC No 231-818-8 Purity > 99.5%
Measured pH Range: 5.9 - 7.3 at 25C
Refractive Index: 1.35835 at 20C
Conductivity Range: 171.4 - 211.1 mS at 25C
Synonyms: Acetic acid sodium salt or Sodium acetate
C2H3NaO2 3H2O or CH3COONa 3H2O Mr 136.08
CAS [6131-90-4] EC No 204-823-8 Purity > 99.5%
HR2-543: Measured pH Range: 9.0 - 9.7 at 25C
Refractive Index Range: 1.36432 - 1.36440 at 20C
Conductivity Range: 73.7 - 79.9 mS at 25C
HR2-763: Titrated to pH 7.0 at 25C using Hydrochloric acid (HR2-581)
Refractive Index Range: 1.37343 - 1.37369 at 20C
Conductivity Range: 74.3 - 77.8 mS at 25C
Synonyms: None NaBr or BrNa Mr 102.89
CAS [7647-15-6] EC No 231-599-9 Purity > 99.0% Measured pH Range: 6.6 - 8.4 at 25C
Refractive Index Range: 1.39146 - 1.39846 at 20C
Conductivity Range: 309.0 - 369.4 mS at 25C
Synonyms: None NaCl Mr 58.44 CAS [7647-14-5] EC No 231-598-3 Purity > 99.5%
Measured pH Range: 5.5 - 8.6 at 25C Refractive Index Range: 1.37712 - 1.37742 at 20C
Conductivity Range: 281.2 - 354.8 mS at 25C
Synonyms: L(+)-Tartaric acid potassium sodium salt or
Rochelle salt or Seignette salt
KOCOCH(OH)CH(OH)COONa 4H2O or C
4
H
4
KNaO
6
4H2O
Mr
282.22 CAS [6381-59-5] EC No 206-156-8 Purity > 99.5%
Measured pH Range: 8.4 - 9.1 at 25C
Refractive Index Range: 1.37582 - 1.37599 at 20C
Conductivity Range: 81.1 - 97.4 mS at 25C
Synonyms: None K2SO4 Mr 174.26
CAS [7778-80-5] EC No 231-915-5 Purity > 99.0%
Measured pH Range: 6.6 - 6.8 at 25C
Refractive Index Range: N/A
Conductivity Range: 70.8 - 77.8 mS at 25C
O
N
H
OH
H
3H
2
O
CH
3
ONa C
O
HO OK P
O
O
O P T I M I Z E


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Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 48
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S A L T S
Sodium formate
HR2-547 7.0 M solution 200 ml $68.00
HR2-765 pH 7.0 - 5.0 M solution 200 ml $69.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl .............................. 0.005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ................................. 0.01%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO4 ........................... 0.005%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
Sodium fluoride
HR2-645 0.8 M solution 100 ml $63.00
Al ............................ 0.0005%
As ......................... 0.00005%
Ba ............................. 0.001%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl .............................. 0.005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ............................. 0.001%
HF ............................... 0.05%
K ................................. 0.02%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
NaOH .......................... 0.04%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SiF
6............................... 0.1%
SO4 ............................. 0.02%
SO3 ........................... 0.005%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
Sodium malonate
HR2-747 pH 4.0 - 3.4 M solution 200 ml $79.00
HR2-749 pH 5.0 - 3.4 M solution 200 ml $79.00
HR2-751 pH 6.0 - 3.4 M solution 200 ml $79.00
HR2-707 pH 7.0 - 3.4 M solution 200 ml $79.00
HR2-807 pH 8.0 - 3.4 M solution 200 ml $79.00
HR2-809 pH 9.0 - 3.4 M solution 200 ml $79.00
Sodium nitrate
HR2-661 7.0 M solution 200 ml $75.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl ............................ 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0002%
IO
3
.......................... 0.0005%
K ............................... 0.005%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
NH
4
+
......................... 0.002%
Ni ............................ 0.0005%
NO2........................... 0.001%
Pb ........................... 0.0005%
PO
4 ......................... 0.0002%
SO4 ........................... 0.003%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
Sodium citrate tribasic dihydrate
HR2-549 1.6 M solution 200 ml $74.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.005%
Cd ........................... 0.0005%
Cl .............................. 0.001%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ................................. 0.01%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
NH4
+
......................... 0.001%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO4 ........................... 0.005%
Zn ........................... 0.0005%
Synonyms: Citric acid trisodium salt dihydrate or Trisodium citrate dihydrate
C6H5Na3O7 2H2O or HOC(COONa)(CH2COONa)2 2H2O
Mr 294.10 CAS [6132-04-3] EC No 200-675-3 Purity > 99.0 %
Measured pH Range: 8.2 - 8.3 at 25C
Refractive Index Range: 1.39431 - 1.39467 at 20C
Conductivity Range: 50.2 - 60.1 mS at 25C
Synonyms: Chile salpeter
NaNO3 Mr 84.99 CAS [7631-99-4] EC No 231-554-3 Purity > 99.0%
Measured pH Range: 5.0 - 6.6 at 25C
Refractive Index Range: 1.38545 - 1.38576 at 20C
Conductivity Range: 174.4 - 195.3 mS at 25C
Sodium phosphate dibasic dihydrate
HR2-639 1.0 M solution 200 ml $94.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl .............................. 0.001%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
N ............................... 0.001%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO4 ........................... 0.005%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
Synonyms: sec-Sodium phosphate or di-sodium hydrogen phosphate dihydrate or
Disodium hydrogen phosphate dihydrate or Disodium phosphate
HNa2O4P 2H2O or Na2HPO4 2H2O Mr 177.99
CAS[10028-24-7] EC No 231-448-7 Purity > 99.0%
Measured pH Range: 8.7 - 8.9 at 25C
Refractive Index Range: 1.35711 - 1.35717 at 20C
Conductivity Range: 62.3 - 69.0 mS at 25C
Synonyms: None NaF Mr 41.99 CAS [7681-49-4] EC No 231-667-8 Purity > 99.0%
Measured pH Range: 8.7 - 9.9 at 25C Refractive Index Range: 1.33713 - 1.33716 at 20C
Conductivity Range: 49.9 - 52.7 mS at 25C
Synonyms: Formic acid sodium salt CHNaO2 or HCOONa
Mr 68.01 CAS [141-53-7] EC No 205-488-0 Purity > 99.0%
HR2-547: Measured pH Range: 8.4 - 9.3 at 25C
Refractive Index Range: 1.38040 - 1.38045 at 20C
Conductivity Range: 107.5 - 120.5 mS at 25C
HR2-765: Titrated to pH 7.0 at 25C using Hydrochloric acid (HR2-581)
Refractive Index Range: 1.36899 - 1.36909 at 20C
Conductivity Range: 121.8 - 134.7 mS at 25C
Synonyms: Propanedioic acid or Malonic acid
C3H4O4 (before titration with NaOH)
Mr 104.06 (before titration with NaOH) CAS [141-82-2] EC No 205-503-0 Purity > 99.0%
HR2-747: Titrated to pH 4.0 at 25C using Sodium hydroxide (HR2-583)
Refractive Index Range: 1.38388 - 1.38458 at 20C Conductivity Range: 64.2 - 74.1 mS at 25C
HR2-749: Titrated to pH 5.0 at 25C using Sodium hydroxide (HR2-583)
Refractive Index Range: 1.39285 - 1.39476 at 20C Conductivity Range: 63.5 - 77.9 mS at 25C
HR2-751: Titrated to pH 6.0 at 25C using Sodium hydroxide (HR2-583)
Refractive Index Range: 1.39940 - 1.39982 at 20C Conductivity Range: 64.0 - 75.5 mS at 25C
HR2-707: Titrated to pH 7.0 at 25C using Sodium hydroxide (HR2-583)
Refractive Index Range: 1.40091 - 1.40112 at 20C Conductivity Range: 66.8 - 77.1 mS at 25C
HR2-807: Titrated to pH 8.0 at 25C using Sodium hydroxide (HR2-583)
Refractive Index Range: 1.40121 - 1.40135 at 20C Conductivity Range: 72.6 - 73.2 mS at 25C
HR2-809: Titrated to pH 9.0 at 25C using Sodium hydroxide (HR2-583)
Refractive Index Range: 1.40119 - 1.40128 at 20C Conductivity Range: 72.2 - 73.1 mS at 25C
Sodium phosphate monobasic monohydrate
HR2-551 4.0 M solution 200 ml $94.00
As ......................... 0.00005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl ............................ 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
N ............................... 0.001%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO4 ........................... 0.003%
Zn ........................... 0.0005%
Synonyms: Monosodium phosphate or
Sodium dihydrogen phosphate monohydrate
H2NaPO4 H2O or NaH2PO4 H2O Mr 137.99 CAS [10049-21-5]
EC No 231-449-2 Purity > 99.0%
Measured pH Range: 3.4 at 25C
Refractive Index Range: 1.38216 - 1.38229 at 20C
Conductivity Range: 58.8 - 60.5 mS at 25C
Sodium sulfate decahydrate
HR2-673 1.0 M solution 200 ml $75.00
Ca ............................. 0.002%
Cd ........................... 0.0005%
Cl ............................ 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.002%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
N ............................. 0.0005%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
PO4 ........................... 0.001%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
Synonyms: Glaubers salt
Na2SO4 10H2O Mr 322.20 CAS [7727-73-3]
EC No 231-820-9 Purity > 99.0%
Measured pH Range: 6.0 - 7.8 at 25C
Refractive Index Range: 1.35184 - 1.35190 at 20C
Conductivity Range: 89.0 - 96.1 mS at 25C
O
NaO
O
O
ONa
ONa
HO
H
2
O
H
2
O
Sodium potassium phosphate (Quik Optimize)
HR2-223 Quik Optimize 100 ml, 2 bottles $175.00
Quik Optimize kit includes:
4.0 M Sodium phosphate monobasic monohydrate, 100 ml (HR2-551)
Mr 137.99 NaH2PO4 H2O CAS [10049-21-5] EC No 231-449-2
4.0 M Potassium phosphate dibasic, 100 ml (HR2-635)
Mr 174.18 K2HPO4 CAS [7758-11-4] EC No 231-834-5
Each kit contains a dilution table to create any pH (between 5.0 and 8.2) and any concentration
(between 0.2 and 4.0 M) in 0.2 increments. This kit can be used to reproduce Quik Screen
(HR2-221) reagent conditions as well as formulate optimization conditions.
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S A L T S
Succinic acid pH 7.0
HR2-709 pH 7.0 - 1.2 M solution 200 ml $94.00
Ca ............................. 0.005%
Cd ........................... 0.0005%
Cl .............................. 0.001%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Mg ............................. 0.001%
Mn ........................... 0.0005%
N ............................... 0.001%
Na ............................. 0.005%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
PO4 ........................... 0.001%
SO4 ........................... 0.003%
Zn ........................... 0.0005%
Tacsimate pH 4, 5, 6, 7, 8, & 9
continued....Tacsimate pH 4, 5, 6, 7, 8, & 9
HR2-823 100% solution pH 4.0 200 ml $88.00
HR2-825 100% solution pH 5.0 200 ml $88.00
HR2-827 100% solution pH 6.0 200 ml $88.00
HR2-755 100% solution pH 7.0 200 ml $88.00
HR2-829 100% solution pH 8.0 200 ml $88.00
HR2-813 100% solution pH 9.0 200 ml $88.00
HR2-839 55% Tacsimate pH 6.0, 0.1 M MES pH 5.8 100 ml $95.00
HR2-843 55% Tacsimate pH 7.0, 0.1 M HEPES pH 6.8 100 ml $95.00
HR2-847 55% Tacsimate pH 8.0, 0.1 M BIS-TRIS pH 7.8 100 ml $95.00
HR2-851 55% Tacsimate pH 8.0, 0.1 M BTP pH 7.8 100 ml $95.00
Zinc acetate dihydrate
HR2-563 1.0 M solution 100 ml $74.00
Ca ............................... 0.005%
Cd ............................... 0.005%
Cl ................................ 0.005%
Co ............................... 0.005%
Cu ............................... 0.005%
Fe ............................... 0.005%
K ................................... 0.01%
Na ................................. 0.01%
Ni ................................ 0.005%
Pb ............................... 0.005%
SO4 ............................. 0.005%
Zinc sulfate heptahydrate
HR2-641 2.0 M solution 100 ml $67.00
As ......................... 0.00001%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl ............................ 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.001%
Mg ........................... 0.0005%
Mn ........................... 0.0002%
N ............................. 0.0005%
Na ............................. 0.001%
Ni ............................ 0.0005%
Pb ............................. 0.001%
Trimethylamine N-oxide dihydrate
Tryptone
HR2-777 1.0 M solution 100 ml $96.00
HR2-835 10% w/v Tryptone 100 ml $69.00
Sodium tartrate dibasic dihydrate
HR2-677 1.5 M solution 200 ml $85.00
Al ............................. 0.0005%
As .......................... 0.00001%
Ba ............................ 0.0005%
Bi ............................. 0.0005%
Ca .............................. 0.001%
Cd ............................ 0.0005%
Cl ............................. 0.0005%
Co ............................ 0.0005%
Cr ............................. 0.0005%
Cu ............................ 0.0005%
Fe ............................ 0.0005%
K ................................ 0.005%
Li .............................. 0.0005%
Mg ............................ 0.0005%
Mn ............................ 0.0005%
Mo ............................ 0.0005%
Ni ............................. 0.0005%
Pb ............................ 0.0005%
PO4 .......................... 0.0005%
SO4 ............................ 0.005%
Sr ............................. 0.0005%
Zn ............................ 0.0005%
Sodium thiocyanate
HR2-693 8.0 M solution 200 ml $75.00
Ca ................................ 0.02%
Cd .............................. 0.005%
Cl ................................. 0.05%
Co .............................. 0.005%
Cu .............................. 0.005%
Fe .............................. 0.005%
K .................................... 0.2%
Ni ............................... 0.005%
Pb .............................. 0.005%
SO
4 .............................. 0.05%
Zn .............................. 0.005%
Zinc chloride
HR2-811 2.0 M Zinc chloride 100 ml $56.00
Ca ............................. 0.001%
Cd ........................... 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.001%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
N ............................. 0.0005%
Na ............................. 0.001%
Ni ............................ 0.0005%
NO3............................. 0.003%
Pb ............................. 0.001%
SO4 ............................. 0.002%
Synonyms: L-(+)-Tartaric acid disodium salt or
Disodium tartrate dihydrate or Sodium tartrate dihydrate
C4H4Na2O6 2H2O Mr 230.08 CAS [6106-24-7]
EC No 212-773-3 Purity > 99.0%
Measured pH Range: 7.5 - 8.8 at 25C
Refractive Index Range: 1.37600 - 1.37625 at 20C
Conductivity Range: 67.9 - 71.0 mS at 25C
Synonyms: TMANO or N,N-Dimethylmethanamine oxide
(CH3)3NO 2H2O Mr 111.14 CAS [62637-93-8]
EC No 214-675-6 Purity > 98.0%
Measured pH Range: 8.0 - 9.3 at 25C
Refractive Index: 1.34327 at 20C
Conductivity Range: 31.0 - 67.1 mS at 25C
Synonyms: Sodium isothiocyanate or Sodium rhodanate or
Sodium rhodanide or Sodium sulfocyanate
NaSCN or CNNaS Mr 81.07 CAS [540-72-7]
EC No 208-754-4 Purity > 98.0%
Measured pH Range: 5.4 - 6.9 at 25C
Refractive Index Range: 1.45963 - 1.46046 at 20C
Conductivity Range: 137.1 - 161.5 mS at 25C
Synonyms: Zinc diacetate
Zn(CH3COO)2 2H2O or C4H6O4Zn 2H2O Mr 219.52
CAS [5970-45-6] EC No 209-170-2 Purity > 98.0%
Measured pH Range: 5.5 - 5.8 at 25C
Refractive Index Range: 1.35739 - 1.35749 at 20C
Conductivity Range: 16.0 - 17.6 mS at 25C
Synonyms: Zinc chloride anhydrous ZnCl
2
Mr
136.30
CAS [7646-85-7] EC No 231-592-0 Purity > 98.0%
Measured pH Range: 3.1 - 4.1 at 25C
Refractive Index Range: 1.37596 - 1.37597 at 20C
Conductivity Range: 90.2 - 104.1 mS at 25C
Synonyms: None ZnSO4 7H2O Mr 287.56
CAS [7446-20-0] EC No 231-793-3 Purity > 99.5%
Measured pH Range: 3.4 - 4.0 at 25C
Refractive Index Range: 1.38259 - 1.38264 at 20C
Conductivity Range: 54.1 - 57.4 mS at 25C
Synonyms: Dicarboxylic acid C4. or Butanedioic acid
C4H6O4 or HOOCCH2CH2COOH Mr 118.09 CAS [110-15-6]
EC No 203-740-4 Purity > 99.5%
pKa
1
= 4.2 at 25C pKa
2
= 5.6 at 25C
Titrated to pH 7.0 at 25C using Sodium hydroxide (HR2-583)
Refractive Index Range: 1.36373 - 1.36407 at 20C
Conductivity Range: 70.1 - 79.7 mS at 25C
O
NaO
O
ONa
OH
2H
2
O
OH H
H
2H
2
O Zn
2+
[ [
2
O
O H
3
C
-
S
N
Na
O
O
S
O
O
Zn
++
Tacsimate is a pH titrated mixture of organic acids: 1.8305 M Malonic acid,
0.25 M Ammonium citrate tribasic, 0.12 M Succinic acid, 0.3 M DL-Malic acid,
0.4 M Sodium acetate trihydrate, 0.5 M Sodium formate, 0.16 M Ammonium tartrate dibasic
HR2-823: Titrated to pH 4.0 at 25C using Sodium hydroxide (HR2-583)
Refractive Index Range: 1.38590 - 1.38655 at 20C Conductivity Range: 73.1 - 79.8 mS at 25C
HR2-825: Titrated to pH 5.0 at 25C using Sodium hydroxide (HR2-583)
Refractive Index Range: 1.39432 - 1.39476 at 20C Conductivity Range: 76.2 - 83.1 mS at 25C
HR2-827: Titrated to pH 6.0 at 25C using Sodium hydroxide (HR2-583)
Refractive Index Range: 1.39981 - 1.40017 at 20C Conductivity Range: 77.4 - 79.9 mS at 25C
HR2-755: Titrated to pH 7.0 at 25C using Sodium hydroxide (HR2-583)
Refractive Index Range: 1.40102 - 1.40171 at 20C Conductivity Range: 75.7 - 90.7 mS at 25C
Continued next column....
HR2-829: Titrated to pH 8.0 at 25C using Sodium hydroxide (HR2-583)
Refractive Index Range: 1.40129 - 1.40166 at 20C Conductivity Range: 74.9 - 79.8 mS at 25C
HR2-813: Titrated to pH 9.0 at 25C using Sodium hydroxide (HR2-583)
Refractive Index Range: 1.40142 - 1.40151 at 20C Conductivity Range: 75.6 - 75.9 mS at 25C
HR2-839: Titrated to pH 5.8 at 25C using Hydrochloric acid (HR2-581)
Refractive Index Range: 1.37622 at 20C Conductivity Range: 94.2 mS at 25C
HR2-843: Titrated to pH 6.8 at 25C using Hydrochloric acid (HR2-581)
Refractive Index Range: 1.37822 at 20C Conductivity Range: 92.6 - 96.0 mS at 25C
HR2-847: Titrated to pH 7.8 at 25C using Hydrochloric acid (HR2-581)
Refractive Index Range: 1.37808 at 20C Conductivity Range: 93.8 mS at 25C
HR2-851: Titrated to pH 7.8 at 25C using Hydrochloric acid (HR2-581)
Refractive Index Range: 1.37949 at 20C Conductivity Range: 93.7 mS at 25C
Measured pH Range: 7.0 - 7.1 at 25C
Refractive Index: 1.35052 at 20C
Conductivity Range: 7.7 - 8.5 mS at 25C
O P T I M I Z E


- R E A G E N T S
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BIS-TRIS propane pH 7.0 Buffer
HR2-795 pH 7.0 - 1.0 M solution 100 ml $119.00
HEPES sodium Buffer (Useful pH Range: 6.6 - 8.5)
HR2-577 1.0 M solution 100 ml $98.00
HR2-733 pH 7.5 - 1.0 M solution 100 ml $98.00
HR2-931-01 pH 6.8 - 1.0 M solution 185 ml $206.00
HEPES Buffer (Useful pH Range: 6.8 - 8.2)
HR2-585 1.0 M solution 100 ml $84.00
HR2-785 pH 7.0 - 1.0 M solution 100 ml $84.00
HR2-729 pH 7.5 - 1.0 M solution 100 ml $84.00
Citric acid pH 3.5 Buffer (Useful pH Range: 2.2 - 6.5)
Citric acid BIS-TRIS propane Buffer (Useful pH Range: 2.5 - 9.5)
HR2-757 pH 3.5 - 1.0 M solution 100 ml $32.00
HR2-831 1.0 M Citric acid 100 ml $33.00
HR2-833 1.0 M BIS-TRIS propane 100 ml $119.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl ............................ 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Na ............................. 0.005%
Ni ............................ 0.0005%
Oxalate (C2O4) ........... 0.05%
Pb ........................... 0.0005%
PO4 ......................... 0.0005%
SO4 ........................... 0.002%
Sr ............................ 0.0005%
Tartrate (C4H4O6) ......... 0.2%
Zn............................. 0.0005%
B U F F E R S
ADA Buffer (Useful pH Range: 5.6 - 7.5)
HR2-507 0.5 M solution 100 ml $88.00
HR2-817 pH 6.5 - 1.0 M solution 100 ml $98.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl .................................. 0.1%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ............................. 0.005%
Na ............................... 0.05%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO4 ........................... 0.005%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
BICINE Buffer (Useful pH Range: 7.4 - 9.3)
HR2-509 1.0 M solution 100 ml $76.00
HR2-723 pH 9.0 - 1.0 M solution 100 ml $76.00
BIS-TRIS Buffer
Ca............................... 0.001%
Cd ........................... 0.0005%
Cl .............................. 0.005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Na ............................. 0.005%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO4 ........................... 0.005%
Zn ........................... 0.0005%
Synonyms: N-(2-Acetamido)iminodiacetic acid or
N-(Carbamoylmethyl)iminodiacetic acid
C6H10N2O5 or H2NCOCH2N(CH2CO2H)2 Mr 190.16
CAS [26239-55-4] EC Number 247-530-0 Purity > 99.0%
pKa = 6.6 at 25C
HR2-507: Titrate to useful pH range using Sodium hydroxide (HR2-583)
Refractive Index: 1.35012 at 20C
Conductivity Range: 23.9 - 28.6 mS at 25C
HR2-817: Titrated to pH 6.5 at 25C using Sodium hydroxide (HR2-583)
Refractive Index Range: 1.36776 - 1.36803 at 20C
Conductivity Range: 42.7 - 43.8 mS at 25C
Synonyms: Citric acid anhydrous
C6H8O7 or HOC(COOH)(CH2COOH)2
Mr 192.12 CAS [77-92-9] EC No 201-069-1 Purity > 99.5%
pKa1 = 3.13 at 25C pKa2 = 4.76 at 25C pKa3 = 6.4 at 25C
HR2-757: Titrated to pH 3.5 at 25C using Sodium hydroxide (HR2-583)
Refractive Index Range: N/A
Conductivity Range: 28.7 - 33.3 mS at 25C
Citrate BIS-TRIS propane (CBTP) is a buffer system useful across pH 2.5 to 9.5. Using CBTP
one can use a single buffer system to screen pH 2.5 to 9.5 by varying the ratio of Citric acid to
BIS-TRIS propane. Although the two buffer reagents are made available separately for conve-
nient, individual replacement, they are designed to be used together, with the supplied CBTP
buffer titration table to create a crystallization buffer system covering pH 2.5 to 9.5.
HR2-831: Measured pH Range: 1.2 - 1.5 at 25C
Refractive Index: 1.35682 at 20C Conductivity Range: 7.8 - 8.7 mS at 25C
HR2-833: Measured pH Range: 11.2 - 11.3 at 25C
Refractive Index: 1.38076 at 20C Conductivity Range: 309.0 - 364.0 mS at 25C
Synonyms: 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid or
N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) or
2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid
C8H18N2O4S Mr 238.31 CAS [7365-45-9] EC No 230-907-9
Purity > 99.0% pKa = 7.5 at 25C
HR2-585: Titrate to useful pH range using Sodium hydroxide (HR2-583)
Measured pH Range: 5.5 - 5.5 at 25C
Refractive Index Range: 1.37121 - 1.37186 at 20C
Conductivity Range: 161.2 - 178.4 mS at 25C
HR2-785: Titrated to pH 7.0 at 25C using Sodium hydroxide (HR2-583)
Refractive Index Range: 1.37161 at 20C Conductivity Range: 7.1 mS at 25C
HR2-729: Titrated to pH 7.5 at 25C using Sodium hydroxide (HR2-583)
Refractive Index Range: 1.37184 at 20C Conductivity Range: 12.1 mS at 25C
Synonyms: HEPES sodium salt or
4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt or
N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) sodium salt
C8H17N2NaO4S Mr 260.29 CAS [75277-39-3] EC No 278-169-7
Purity > 99.5% pKa = 7.5 at 25C
HR2-577: Titrate to useful pH range using Hydrochloric acid (HR2-581)
Measured pH Range: 10.5 - 10.9 at 25C
Refractive Index Range: N/A
Conductivity Range: 25.9 - 28.2 mS at 25C
HR2-733: Titrated to pH 7.5 at 25C using Hydrochloric acid (HR2-581)
Refractive Index Range: 1.37713 - 1.37743 at 20C
Conductivity Range: 40.6 - 42.1 mS at 25C
HR2-931-01: HEPES sodium pH 6.8 - 1.0 M solution is a Custom Shop reagent and is used to
reproduce and optimize Crystallization Reagents for use with the Silver Bullets kits.
Synonyms: N,N-Bis(2-hydroxyethyl)glycine
C6H13NO4 Mr 163.17 CAS [150-25-4] EC No 205-755-1
Purity > 99.0% pKa = 8.3 at 25C
HR2-509: Titrate to useful pH range using Sodium hydroxide (HR2-583)
Measured pH Range: 5.1 - 5.3 at 25C
Refractive Index Range: 1.35805 - 1.35821 at 20C
Conductivity Range: 114.3 - 114.7 mS at 25C
HR2-723: Titrated to pH 9.0 at 25C using Sodium hydroxide (HR2-583)
Refractive Index Range: 1.36078 - 1.36104 at 20C
Conductivity Range: 24.3 - 24.7 mS at 25C
Synonyms: 2,2-Bis(hydroxymethyl)-2,2',2"-nitrilotriethanol or
2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol or
Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane
C8H19NO5 Mr 209.24 CAS [6976-37-0] EC No 230-237-7
Purity > 99.0% pKa = 6.4 at 20C pKa = 6.5 at 25C
HR2-781: Titrated to pH 5.5 at 25C using Hydrochloric acid (HR2-581)
Refractive Index Range: 1.37130 - 1.37150 at 20C
Conductivity Range: 38.4 - 43.1 mS at 25C
HR2-783: Titrated to pH 6.5 at 25C using Hydrochloric acid (HR2-581)
Refractive Index Range: 1.36860 - 1.36926 at 20C
Conductivity Range: 22.9 - 28.7 mS at 25C
HR2-906-24: BIS-TRIS pH 7.8 - 1.0 M solution is a Custom Shop reagent and is used to
reproduce and optimize Crystallization Reagents for use with the Silver Bullets kits.
Synonyms: 1,3-Bis[tris(hydroxymethyl)methylamino]propane
CH2[CH2NHC(CH2OH)3]2 or C11H26N2O6
Mr 282.34 CAS [64431-96-5] EC No 264-899-3
Purity > 99.0% pKa1 = 6.8 at 25C pKa2 = 9.0 at 25C
HR2-795: Titrated to pH 7.0 at 25C using Hydrochloric acid (HR2-581)
Refractive Index Range: 1.39139 - 1.39143 at 20C
Conductivity Range: 41.7 - 42.5 mS at 25C
N
OH
O
HO
O
NH2 O
N N
S OH OH
O
O
HR2-781 pH 5.5 - 1.0 M solution 100 ml $124.00
HR2-783 pH 6.5 - 1.0 M solution 100 ml $124.00
HR2-906-24 pH 7.8 - 1.0 M solution 185 ml $151.00
51
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B U F F E R S
MES monohydrate Buffer (Useful pH Range: 5.2 - 7.1)
HR2-587 0.5 M solution 100 ml $98.00
HR2-787 pH 6.5 - 1.0 M solution 100 ml $98.00
HR2-943-07 pH 5.8 - 1.0 M solution 185 ml $138.00
Ca ............................. 0.005%
Cd ........................... 0.0005%
Cl .............................. 0.005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Na ............................. 0.005%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO4 ........................... 0.005%
Zn ........................... 0.0005%
Synonyms: MES or 2-(N-morpholino)ethanesulfonic acid or
4-morpholineethanesulfonic acid monohydrate
C6H13NO4S H2O Mr 213.25 CAS [145224-94-8]
EC No 224-632-3 Purity > 99.0% pKa = 6.1 at 25C
HR2-587: Titrate to useful pH range using Sodium hydroxide (HR2-583)
Measured pH Range: 3.2 - 3.4 at 25C
Refractive Index Range: 1.34690 - 1.34731 at 20C
Conductivity Range: 166.4 - 259.0 mS at 25C
HR2-787: Titrated to pH 6.5 at 25C using Sodium hydroxide (HR2-583)
Refractive Index Range: 1.36197 - 1.36229 at 20C
Conductivity Range: 19.2 - 25.6 mS at 25C
HR2-943-07: MES monohydrate pH 5.8 - 1.0 M solution is a Custom Shop reagent and is used to
reproduce and optimize Crystallization Reagents for use with the Silver Bullets kits.
Sodium cacodylate trihydrate Buffer (Useful pH Range: 5.0 - 7.4)
HR2-575 1.0 M solution 100 ml $178.00
HR2-737 pH 6.5 - 1.0 M solution 100 ml $178.00
Synonyms: Cacodylic acid sodium salt trihydrate or
Dimethylarsinic acid sodium salt or Dimethylarsonic acid sodium salt
C2H6AsNaO2 3H2O or (CH3)2AsO2Na 3H2O Mr 214.03
CAS [6131-99-3] EC No 204-708-2 pKa = 6.2 at 25C Purity > 98.0%
HR2-575: Titrate to useful pH range using Hydrochloric acid (HR2-581)
Measured pH Range: 8.3 - 8.5 at 25C
Refractive Index: 1.35596 at 20C
Conductivity Range: 33.3 - 37.7 mS at 25C
HR2-737: Titrated to pH 6.5 at 25C using Hydrochloric acid (HR2-581)
Refractive Index Range: 1.35651 - 1.35707 at 20C
Conductivity Range: 43.5 - 45.4 mS at 25C
Imidazole Buffer (Useful pH Range: 6.2 - 7.8)
HR2-573 1.0 M solution 100 ml $68.00
HR2-819 pH 7.0 - 1.0 M solution 100 ml $82.00
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl .............................. 0.005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Na ............................. 0.005%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO4 ................................ 0.005%
Zn ........................... 0.0005%
Synonyms: 1,3-Diaza-2,4-cyclopentadiene or Glyoxaline
C3H4N2 Mr 68.08 CAS [288-32-4] EC No 206-019-2
Purity > 99.5% pKa = 6.95 at 25C
HR2-573: Titrate to useful pH range using Hydrochloric acid (HR2-581)
Measured pH Range: 10.2 - 10.6 at 25C
Refractive Index: 1.34423 at 20C
Conductivity Range: 72.2 - 85.2 mS at 25C
HR2-819: Titrated to pH 7.0 at 25C using Hydrochloric acid (HR2-581)
Refractive Index Range: 1.34847 - 1.34872 at 20C
Conductivity Range: 46.1 - 49.2 mS at 25C
Sodium citrate tribasic dihydrate Buffer (Useful pH Range: 3.0 - 6.2)
HR2-571 1.0 M solution 100 ml $41.00
HR2-735 pH 5.6 - 1.0 M solution 100 ml $41.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.005%
Cd ........................... 0.0005%
Cl .............................. 0.001%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ................................. 0.01%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
NH
4
+
......................... 0.001%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO
4 ................................ 0.005%
Zn ........................... 0.0005%
Synonyms: Citric acid trisodium salt dihydrate or
Trisodium citrate dihydrate C6H5Na3O7 2H2O or
HOC(COONa)(CH2COONa)2 2H2O Mr 294.10
CAS [6132-04-3] EC No 200-675-3 Purity > 99.0%
pKa1 = 3.1 at 25C pKa2 = 4.8 at 25C pKa3 = 5.4 at 25C
HR2-571: Titrate to useful pH range using Hydrochloric acid (HR2-581)
Measured pH Range: 8.1 - 8.3 at 25C Refractive Index: 1.37370 at 20C
Conductivity Range: 60.0 - 67.5 mS at 25C
HR2-735: Titrated to pH 5.6 at 25C using Hydrochloric acid (HR2-581)
Refractive Index Range: 1.37423 - 1.37502 at 20C
Conductivity Range: 67.4 - 72.4 mS at 25C
O
NaO
O
O
ONa
ONa
HO
H
2
O
H
2
O
Sodium acetate trihydrate Buffer (Useful pH Range: 3.6 - 5.6)
HR2-569 1.0 M solution 100 ml $29.00
HR2-789 pH 4.5 - 1.0 M solution 100 ml $29.00
HR2-731 pH 4.6 - 1.0 M solution 100 ml $29.00
Al ............................ 0.0005%
As ......................... 0.00001%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl ............................ 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
PO4 ......................... 0.0005%
SO4 ........................... 0.002%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
Synonyms: Acetic acid sodium salt C2H3NaO2 3H2O or
CH3COONa2 3H2O M
r
136.08 CAS [6131-90-4]
EC No 204-823-8 Purity > 99.5% pKa = 4.8 at 25C
HR2-569: Titrate to useful pH range using Hydrochloric acid (HR2-581)
Measured pH Range: 8.7 - 9.2 at 25C
Refractive Index Range: 1.34405 - 1.34408 at 20C
Conductivity Range: 48.0 - 51.7 mS at 25C
HR2-789: Titrated to pH 4.5 at 25C using Hydrochloric acid (HR2-581)
Refractive Index Range: 1.34558 - 1.34564 at 20C
Conductivity Range: 60.8 - 67.7 mS at 25C
HR2-731: Titrated to pH 4.6 at 25C using Hydrochloric acid (HR2-581)
Refractive Index Range: 1.34553 - 1.34556 at 20C
Conductivity Range: 59.3 - 63.4 mS at 25C
3H
2
O
CH
3
ONa C
O
Tris Buffer (Useful pH Range: 7.0 - 9.0)
HR2-589 1.0 M solution 100 ml $44.00
HR2-725 pH 8.5 - 1.0 M solution 100 ml $44.00
Al ............................ 0.0005%
As ......................... 0.00001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Cl ............................ 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Na ............................. 0.005%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO
4
......................... 0.0005%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
Synonyms: Trizma

base or THAM or Tris base or


2-Amino-2-(hydroxymethyl)-1,3-propanediol or
Tris(hydroxymethyl)aminomethane or Trometamol
NH2C(CH2OH)3 or C4H11NO3 Mr 121.14 CAS [77-86-1]
EC No 201-064-4 Purity > 99.8% pKa = 8.1 at 25C
HR2-589: Titrate to useful pH range using Hydrochloric acid (HR2-581)
Measured pH Range: 10.9 - 11.0 at 25C
Refractive Index Range: 1.35044 - 1.35048 at 20C
Conductivity Range: 197.0 - 223.0 mS at 25C
HR2-725: Titrated to pH 8.5 at 25C using Hydrochloric acid (HR2-581)
Refractive Index Range: 1.35364 - 1.35369 at 20C
Conductivity Range: 18.1 - 21.5 mS at 25C
O P T I M I Z E


- R E A G E N T S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 52
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Synonyms: TRIS HCl or Trizma

hydrochloride or
Tris(hydroxymethyl)aminomethane hydrochloride
NH2C(CH2OH)3 HCl or C4H11NO3 HCl
Mr 157.60 CAS [1185-53-1] EC No 214-684-5
Purity > 99.0% pKa = 8.1 at 25C
HR2-579: Titrate to useful pH range using Sodium hydroxide (HR2-583)
Measured pH Range: 3.9 - 4.1 at 25C Refractive Index: 1.35882 at 20C
Conductivity Range: 49.1 - 53.3 mS at 25C
HR2-727: Titrated to pH 8.5 at 25C using Sodium hydroxide (HR2-583)
Refractive Index: 1.35961 at 20C Conductivity: 60.3 mS at 25C
TRIS hydrochloride Buffer (Useful pH Range: 7.0 - 9.0)
HR2-579 1.0 M solution 100 ml $45.00
HR2-727 pH 8.5 - 1.0 M solution 100 ml $45.00
Al ............................ 0.0005%
As ........................... 0.0001%
Ba ........................... 0.0005%
Bi ............................ 0.0005%
Ca ............................. 0.001%
Cd ........................... 0.0005%
Co ........................... 0.0005%
Cr ............................ 0.0005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ............................... 0.005%
Li ............................. 0.0005%
Mg ........................... 0.0005%
Mn ........................... 0.0005%
Mo ........................... 0.0005%
Na ............................. 0.005%
Ni ............................ 0.0005%
Pb ........................... 0.0005%
SO
4
........................... 0.005%
Sr ............................ 0.0005%
Zn ........................... 0.0005%
B U F F E R S
Hydrochloric acid
HR2-581 1.0 M solution 100 ml $27.00
Ag ....................... 0.000001%
Al ........................ 0.000005%
As ..................... 0.0000005%
Ba ....................... 0.000002%
Bi .......................... 0.00001%
Br .............................. 0.005%
Ca ......................... 0.00005%
Cd ....................... 0.000001%
Co ....................... 0.000001%
Cr ........................ 0.000002%
Cu ....................... 0.000002%
Fe ......................... 0.00002%
Hg ....................... 0.000001%
K ........................... 0.00001%
Li ......................... 0.000001%
Mg ......................... 0.00001%
Mn ....................... 0.000001%
Mo ....................... 0.000002%
Na ......................... 0.00005%
NH4
+
....................... 0.0001%
Ni ........................ 0.000002%
PO
4 ......................... 0.0005%
Pb ....................... 0.000002%
SO4 ......................... 0.0001%
SO3 ......................... 0.0001%
Sr ........................ 0.000001%
TI ........................ 0.000005%
Zn ....................... 0.000005%
Synonyms: None HCl Mr
36.46 CAS [7647-01-0] EC No 231-595-7
Conductivity Range: 320.4 - 378.2 mS at 25C
Sodium hydroxide
HR2-583 1.0 M solution 100 ml $27.00
Al ............................ 0.0005%
Ca ........................... 0.0005%
Cd ........................... 0.0005%
Cl .............................. 0.005%
Cu ........................... 0.0005%
Fe ........................... 0.0005%
K ................................. 0.02%
Mg ........................... 0.0005%
P ............................. 0.0005%
Pb ............................. 0.001%
SO
4 ............................. 0.05%
Zn ........................... 0.0005%
Synonyms: Caustic soda NaOH or HNaO Mr 40.00 CAS [1310-73-2]
EC No 215-185-5 Purity > 98.0%
d e s c r i p t i o n
Non Detergent Sulfobetaines (NDSB) are a new family of non denaturing
protein solubilizing agents with a wide range of applications. NDSBs have
been used for protein extraction, solubilization, and crystallization. NDSBs
are zwitterionic, they can be removed by dialysis since they do not form
micelles, and they do not alter the biophysical properties of biological
buffers.
This particular class of reagent was developed by Laurent Vuillard
(University of Cambridge, Department of Pathology, UK) in collaboration
with T. Rabilloud (CENG, Grenoble, France).
References:
1. Vuillard, L., T. Rabilloud, M.E. Goldberg, A New Additive for Protein Crystallization., FEBS Letters., 1994.,
353(3):, 294-296.
2. Vuillard, L., et al., Protein crystallography with non detergent sulfobetaines., J. Cryst. Growth. (1996) 168,
150-154.
3. Vuillard, L., Rabilloud, T., Goldberg, M.E., Eur. J. Biochem., (1998) 256, 1, 128-135.
4. http://www.path.cam.ac.uk/~lv213/
Crystal picture of RPA in an earring motif.
Courtesy of Gloria Borgstahl.
University of Toledo
Department of Chemistry
NDSB-195
HR2-703 powder 5 g $98.00
NDSB-201
HR2-701 powder 5 g $27.00
NDSB-221
HR2-791 powder 5 g $133.00
NDSB-211
HR2-793 powder 5 g $181.00
NDSB-256
HR2-705 powder 5 g $98.00
S O L U B I L I Z I N G A G E N T S
w H a T a r e n d s b s ?
Synonyms: Dimethylethylammonium propane sulfonate or
Non-detergent sulfobetaine C
7
H
17
NO
3
S Mr 195.3
Purity > 95.0% by TLC
Synonyms: 3-(1-Pyridino)-1-propane sulfonate
C
8
H
11
NO
3
S Mr 201.2
CAS Number 15471-17-7 EC No 2394913
Purity > 99.0%
Synonyms: Dimethyl-2(-Hydroxyethyl)-(3-Sulfopropyl)-ammonium,
Inner Salt or Non detergent sulfobetaine 211
C
7
H
17
NO
4
S Mr 211.28 Purity > 99.0%
Synonyms: Non-detergent sulfobetaine 221 or
3-(1-Methylpiperidinium)-1-propane Sulfonate
C
9
H
19
NO
3
S Mr 221.3 Purity > 99.0%
Synonyms: Dimethylbenzylammonium propane sulfonate
C
12
H
19
NO
3
S Mr 257.4
Purity > 99.0% by TLC
53
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d e s c r i p t i o n
Try using TCEP hydrochloride instead of dithiothreitol (DTT) as a
reducing agent in your crystallization setups. TCEP hydrochloride
is soluble in water to 310 g per L. TCEP hydrochloride is Tris
(2-carboxyethyl)phosphine hydrochloride. It is an odorless (non-
volatile) reducing agent that is more stable and effective than DTT
or 2-Mercaptoethanol. Unlike DTT, TCEP hydrochloride retains
its reducing power at acid pHs (pH 5) and at pHs above 7.5. It
is unreactive toward other functional groups found in proteins.
Unlike DTT (dithiothreitol), TCEP hydrochloride does not contain a free thiol, and therefore does
not require removal before reaction with a thiol-reactive reagents. It reduces disulfides but apparently
not mercurythiol bonds. TCEP hydrochloride is compatible with many heavy atoms and may be used
during heavy atom derivatization. It is more stable at a higher pH and at higher temperatures than is
DTT and for a longer period of time in buffers without metal chelators such as EGTA.
References
1. Tris(2-carboxyethyl)phosphine stabilization of RNA: comparison with dithiothreitol for use with nucleic acid and thiophosphoryl chemistry. Rhee SS, Burke DH.
Anal Biochem 325, 137-43 (2004) PN51541.
2. A comparison between the sulfhydryl reductants tris(2-carboxyethyl)phosphine and dithiothreitol for use in protein biochemistry. Getz EB, Xiao M, Chakrabarty
T, Cooke R, Selvin PR. Anal Biochem 273, 73-80 (1999) PN34481
3. Burns, J.A., et al. (1991). Selective reduction of disulfides by tris-(2-carboxyethyl)-phosphine. J. Org. Chem. 56, 2648-2650
4. Oda , Y., et al. (2001). Nature Biotech19, 379-382
n
Stable reducing agent for crystallization trials

n
Highly soluble
n
Odorless and non-volatile
n
Effective at acidic and alkaline pH

a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR2-651 TCEP hydrochloride 1 g $46.00
HR2-801 TCEP hydrochloride 10 g $290.00
P OH
O
HO
O
OH O
HCl

H
2
O
2 RSH
R R S S
P OH
O
HO
O
OH O
HCl

O
T C e P H y d r o C H l o r I d e
s I l I C a H y d r o G e l

O P T I M I Z E

- R E D U C I N G A G E N T
Hanging drop vapor diffusion crystal.
Ertugrul Cansizoglu, University of Texas, Southwestern, USA.
O P T I M I Z E

- C R Y O P R O T E C T A N T S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 54
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Paratone-N is a viscous cryoprotectant for both small and large
molecule crystallography.
1
Mildly air-unstable, small molecule com-
pounds can be coated with Paratone-N under an inert atmosphere.
The Paratone-N protected crystal sample can be cryocooled in a
chilled nitrogen gas stream. Paratone-N has also been used suc-
cessfully as a cryoprotectant for biological macromolecule crystals.
It is used during cryocrystallography to displace and reduce the
amount of water (mother liquor, reagent) on the crystal after the
crystal is mounted in a cryoloop. Coating the crystal with Paratone-N can minimize evaporation from
the crystal and reduce exposure and slow diffusion of air (oxygen) to the crystal.
Paratone-N is also known as Parabar 10312, Paratone 8277, and Infineum V8512.
References
1. H. Hope, Cryocrystallography of biological macromolecules: a generally applicable method. Acta Cryst. (1988) B44, 22-26.
2. Structure of the ligand-binding domain (LBD) of human androgen receptor in complex with a selective modulator LGD2226. F. Wang, X.- Liu, H. Li, K.- Liang,
J. N. Miner, M. Hong, E. A. Kallel, A. van Oeveren, L. Zhi and T. Jiang. Acta Cryst. (2006). F62, 1067-1071
3. H. Hope, Annu. Rev. Biophys. Chem. 1990 19:107-126
4. S. Parkin and H. Hope, J. Appl. Cryst. (1998) pages 945-953
n
Cryoprotectant

a p p l i c a t i o n
Order Information
Cat. No. Name Description Price
HR2-643 Paratone-N 100 ml $55.00
P A R A T O N E - N
d e s c r i p t i o n
Used during cryocrystallography to displace and reduce the
amount of water (mother liquor, reagent) on the crystal after the
crystal is mounted in a cryoloop. Coating the crystal with oil can
minimize evaporation from the crystal and reduce exposure and
slow diffusion of air (oxygen) to the crystal.
References
1. Structure of the open form of Aspergillus nidulans 3-dehydroquinate synthase at 1.7 resolution. C.E.
Nichols, A.R. Hawkins and D.K. Stammersa. Acta Crystallographic Section D, Volume 60, Part 5, Pages 971-973, May 2004.
2. H. Hope, Annu. Rev. Biophys. Chem. 1990 19:107-126
n
Cryoprotectant

n
Perfluoropolyether oil
n
Low viscosity
n
Low surface tension
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR2-814 Perfluoropolyether PFO-X175/08 1 ml $25.00
P e r F l u o r o P o l y e T H e r P F o - x 1 7 5 / 0 8
Trypsin jungle.
Allan DArcy, Switzerland.
s I l I C a H y d r o G e l

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- O I L S
d e s c r i p t i o n
Floating Drop Vapor Diffusion
The floating drop vapor diffusion technique
1
has been described
using Fluorinert FC-70. The transparent and high density FC-70
liquid is not miscible with most crystallization reagents, samples,
oil, or water. When Fluorinert liquid and the sample/reagent are
mixed, the sample/reagent drop immediately separates from
the Fluorinert and floats on top of the Fluorinert (see figure 1).
Crystals grown using the floating drop technique do not stick
to the crystallization plate. As the sample/reagent/crystal is not
miscible with the Fluorinert liquid, complete separation of the crystal from the Fluorinert is straight
forward. Crystals grown using the floating drop technique can be removed from the drop without
mechanical damage. Drop volumes can vary widely using the floating drop method. Handling of the
crystal is improved.
Floating and Stirring Technique (FAST)
The floating and stirring technique (FAST)
2
has been described using Fluorinert FC-70 liquid. The
method, which involves a sample and reagent mixture applied over a non-miscible dense liquid
(Fluorinert FC-70) without contact and slow stirring can also be combined with slow cooling or warm-
ing (See figure 2). The floating and stirring method has been reported to accelerate growth of the
crystal as well as prevent subsequent spontaneous nucleation. The method has been used with seed
crystals to promote the growth of a larger crystal without the appearance of subsequent crystals.
Fluorinert: FC-70 Fluid
Mr: 820
CAS number: [86508-42-1]
Pour point: -25C
Boiling point: 215C
Nonflammable
Vapor pressure: 15 pascals
Liquid density: 1,940 kg/m3
Kinematic viscosity: 12 centistokes
Absolute viscosity: 24 centipoise
Coeffecient of expansion: 0.0010C-1
Surface tension: 18 dynes/cm
Refractive index: 1.303
Water solubility: 8 ppmw
Ozone depletion potential: 0
Dielectric strength: 40 kV, 0.1 gap
Dielectric constant: 1.98
Electrical resistivity: 2.3 x 1015 ohm cm
Appearance: Clear, colorless
References
1. Application of a two-liquid system to sitting-drop vapour-diffusion protein crystallization. Adachi, H. et al, Acta Cryst. (2003) D59, 194-196
2. Promotion of large protein crystal growth with stirring solution. Adachi, H. et al. Jpn. J. Appl. Phys. Vol. 41 (2002) pp.1025-1027
3. Two-liquid hanging-drop vapour-diffusion technique of protein crystallization. Hiroaki Adachi et al. Japanese Journal of Applied Physics.
Vol. 43, No. 1A/B, 2004, pp.L79-L81.
n
Drop floating crystallization

n
Viscosity similar to water with approximately
75% greater density
n
Stable
n
Completely fluorinated
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR2-797 100% Fluorinert FC-70 Fluid 100 ml $99.00
F L U O R I N E R T

F C - 7 0 F L U I D
figure 2
Floating and stirring technique
Fluorinert
Protein Solution
Stir Bar
figure 1
Floating drop vapor diffusion technique
Crystallization Drop
Reservoir Solution
Fluorinert
O P T I M I Z E

- O I L S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 56
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d e s c r i p t i o n
Oils used for microbatch and modified microbatch crystallization
under oil.
Al's Oil is a 50:50 (volume:volume) mixture of Paraffin Oil and
Silicon Oil.
Paraffin oil
Synonym: Mineral oil
CAS Number [8042-47-5]
EC Number 2324558
RTECS PY8047000
Appearance Colorless, clear, viscous liquid
Refractive Index n20/D 1.467 (literature) n20/D 1.468
IR Spectroscopy Grade
Density 0.85 g/ml at 20C
Viscosity 40-42 CST at 25C
References
1. The advantages of using a modified microbatch method for rapid screening of protein crystallization conditions. Allan D'Arcy et al. Acta Cryst. (2003).
D59, 396-399
Containerless crystallization is a micromethod of batch crystalliza-
tion of proteins under conditions where the sample and precipi-
tant have no contact with the surface of the crystallization plate.
Drops (2 to 20 l) of sample combined with crystallization reagent
are pipetted at the interface between two layers of inert and immis-
cible silicon oils contained in a 24, 48, or 96 well plate and sealed.
Plates and plain cover slides are available separately. It is easier to
position drops into the center of the oil using plates with larger
reservoirs (i.e. 24 and 48 well plates) but this requires more oil. When using the 96 well plates, drops of
higher density (polymer based reagents such as PEG) tend to migrate to the side walls of the plate.
The method relies upon the use of two immiscible oils (FMS and DMS). Poly-3, 3, 3-Trifluoropropyl-
methylsiloxane (FMS), a branched silicone compound, has a higher density than Polydimethylsiloxane
(DMS). Therefore, one can create a bilayer with a distinct interface by placing the two oils in a reservoir.
Neither DMS nor FMS is miscible with water. The oils are compatible with most crystallization reagents
including but not limited to buffers, salts, polymers, and MPD. Additional protein or crystallization
reagents can be added to the drop in the oil. Crystal mounting as well as seeding can be performed
while the drop is in the oil. During crystal mounting, the drop will maintain a spherical shape and
decrease in size as sample is withdrawn. During seeding, crystal seeds will sediment to the bottom of
the drop. It has been observed that large, perfect, single crystals can be grown using the containerless
crystallization method. Crystals typically nucleate at the oil/water interface and grow into the drop.
n
Microbatch crystallization
n
Screen different temperatures without
condensation
n
Protect the sample from oxidation
n
Under oil crystallization

n
Contact free crystallization by microbatch
under oil
n
Utilizes two immiscible oils to create a
simulated containerless system
a p p l i c a t i o n
a p p l i c a t i o n
f e a t u r e s
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR3-411 Paraffin Oil 100% - 250 ml $40.00
HR3-421 Paraffin Oil 100% - 1 L $100.00
HR3-415 Silicon Oil 100% - 250 ml $150.00
HR3-423 Silicon Oil 100% - 1 L $350.00
HR3-413 Al's Oil (50:50 Paraffin:Silicon) - 250 ml $90.00
HR3-417 Combo Oil Pack (Paraffin, Als, & Silicon Oil) - 250 ml of each $225.00
Order Information
Cat. No. Name Description Price
HR2-593 DMS Oil 100 ml $73.00
HR2-595 FMS Oil 100 ml $171.00
M I C r o b a T C H C r y s T a l l I z a T I o n o I l s
FMS Oil (Lower):
M
r
: 2350
D: 1.25
cSt: 300
Ref Index: 1.381
DMS Oil (Upper):
M
r
: 410
D: 0.873
cSt: 2
Ref Index: 1.390
C O N T A I N E R L E S S C R Y S T A L L I Z A T I O N
CH
3
CH
3
CH
3
F
CH
3 Si Si Si
Si
CH
3
CH
3
CH
3
CH
3
O O CH
3
F F
( (
n
CH
3
CH
3
CH
3
CH
3 Si Si Si
CH
3
CH
3
CH
3
O O CH
3
( (
n
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Gels are a very efficient media for growing macromolecular crys-
tals.
1-5
Silica gels in particular have the advantage in that they are
stable, usable over a wide range of temperatures (0-60C), and are
compatible with a wide variety of precipitants and additives used
for crystal growth.
Gels can reduce nucleation and sedimentation, provide added sta-
bility, and allow crystals to grow larger. The porous network mini-
mizes natural convection. Crystals are suspended in the gel net-
work so that they do not form sediments and can grow free from
strain exerted by the container or other crystals. Heterogeneous
and secondary nucleation are reduced in the presence of a Silica Hydrogel.
3
The Silica Hydrogel can
be used for liquid-gel, liquid-gel-liquid, vapor diffusion, as well as dialysis crystallization methodolo-
gies. The gel is compatible with a wide range of salts, polymers, organic solvents, and buffers used for
macromolecular crystallization in a pH range from 3 to 10.
Each Silica Hydrogel kit contains 12 tubes of Sodium silicate solution and 12 tubes of Acetic acid solu-
tion, 500 l each. All solutions are sterile filtered and formulated using ultra-pure water. Crystallization
accessories are sold separately.
References
1. Robert, M.C. & Lefaucheux, F., J. Crystal Growth (1988) 90, 358.
2. Provost, K. & Robert, M.C., J. Crystal Growth (1991) 110, 258.
3. M.C. Robert, K. Provost, & F. Lefaucheux, Crystallization of Nucleic Acids and Protein, A Practical Approach, Oxford Univ Press (1992) 127-143.
4. McPherson A., Methods in Enzymology (1985) 114, 112.
5. Cudney, B., Patel, S., McPherson, A., Acta Cryst. (1994) D50, 479-483.
n
Quick & easy kit format for crystallization in
gels
n
Proprietary Silica Hydrogel formulation with
neutral pH
n
Gel matrix can reduce nucleation &
sedimentation
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR2-310 Silica Hydrogel 500 l, 24 tubes $95.00
O P T I M I Z E

- s I l I C a H y d r o G e l k I T
d e s c r i p t i o n
Low melting (LM) agaroses are the result of a derivatization process
by organic synthesis. The main properties of these agaroses are
their low melting and gelling temperatures when compared with
standard agaroses. LM agaroses have higher clarity (gel transpar-
ency) than gels of standard agaroses. The gelling temperature of
LM agaroses is 24 to 28C.
The structure of the polysaccharide is that of a galactan, formed by linking agarobioses by links 1-3, 1-4.
This chemical structure gives agaroses the capacity to form strong gels even at low temperatures. The
gels have a macroreticular structure with a very open mesh which can be adjusted simply by varying
the concentration of the agarose. The macroreticule structure of the agarose gel is formed by hydrogen
bonds, which makes the gel reversible, transforming the gel into a solution by heating. The absence
of ionic groups makes the gel a neutral structure. With no interaction, macromolecules can migrate
through the gel mesh, making the gel an efficient sieve for biological macromolecules.
n
Crystallization in agarose gel
n
Gel matrix can reduce nucleation and sedi-
mentation
n
Crystallization grade
n
Low melting agarose
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR8-092 LM Agarose 10 g $58.00
O P T I M I Z E

- L M A G A R O S E
O P T I M I Z E

- I Z I T C R Y S T A L D Y E


Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 58
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Differentiate protein crystals from salt crystals
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0.22 micron sterile filtered solution
n
Small molecule dye penetrates solvent
channels of macromolecular crystals,
coloring the crystals blue.
n
Salt crystals cannot absorb Izit and remain
colorless

a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR4-710 Izit Crystal Dye 0.5 ml vial $15.00
A crystal! Is it protein or is it salt? Have you ever asked yourself this
question? Sure you could mount the crystal in question and take a
quick look at the diffraction pattern but perhaps youre too lazy or
you dont have an x-ray facility. Well, theres always the crush test.
One simply takes a MicroTool or Crystal Probe and crushes
the suspect crystal. A click or solid crunch is indicative of salt while
a powder or silent destruction might indicate one just destroyed a
perfectly good protein crystal. Izit is here to help. Simply place one
l of Izit in the sample drop and wait for an hour or so. Izit is a small
molecule dye which will fill the solvent channels in protein crystals,
coloring the crystals blue. With the appropriate dilution, Izit will in
fact leave a clear drop with blue crystals, as illustrated in the picture
to the right. Salt crystals do not possess these large solvent channels.
Therefore, Izit cannot enter the crystal, leaving one with a clear crystal
and a blue drop. Izit is especially nice for small microcrystals or ques-
tionable precipitate. The 0.5 ml vial of Izit is sufficient for more than
thousands of crystallization drops.
Each Izit vial contains 0.5 ml of Izit dye. All solutions are formulated using ultra-pure water and are sterile
filtered. Crystallization accessories are sold separately.
d e s c r i p t i o n
Picturesque crystal of multidrug efflux pump AcrB.

Markus Seeger, University Zrich, Switzerland.
Nuclear receptor crystal.
Gilbert Bey, ALIX, Illkirch, France.
Crystals of Thermus thermophilus enolase.
From the group of Paola Fucini,
Max Planck Institute for Molecular Genetics, Berlin, Germany.
Protein crystals.
Kasumi Kobayashi, Taiji Nakae and Hiroyuki Akama.
The Kitasato Institute, Kanagawa, Japan.
s t o c k o p t i o n s

k i t s
Protein crystal.
Alexey Rak, Max-Planck-Institut fysiologie, Department of Physical Biochemistry, Dortmund, Germany.
T A B L E O F C O N T E N T S
P A G E S
62 s t o c k o p t i o n s

s a l t
63 s t o c k o p t i o n s

p h
64 - 67 s t o c k o p t i o n s

b u f f e r
68 s t o c k o p t i o n s

- c r y o p r o

n
Crystallization grade salt reagent stock
solutions for screen formulation and
optimization
n
Preformulated and sterile filtered
n
Easy transition from screening to optimization
n
Synergistic with Hampton Research screens,
kits, & reagents
n
49 unique salts, including organic acids
n
Highly concentrated, ready to dilute
a p p l i c a t i o n
f e a t u r e s
d e s c r i p t i o n
StockOptions reagent kits are reagent tool boxes for the
macromolecular crystal grower. They offer precisely for-
mulated, high quality crystallization grade reagent stocks
in convenient, cost-effective kits. The chemicals utilized
in these kits are the same crystallization grade, ultra-high
purity chemicals utilized in the Hampton Research kits such
as Crystal Screen and Crystal Screen 2. StockOptions
reagents are carefully formulated under strict quality stan-
dards to ensure reliable performance and lot-to-lot consis-
tency.
Each reagent in a StockOptions kit is available in convenient concentration, making crystal setups quick
and easy. Gone is the tedious task of finding and sourcing reagents, as well as costly and time-consuming
reagent formulation.
Preformulated reagents also reduce the activation energy between the discovery of preliminary screen
conditions and the task of setting experiments for optimization. The generation of custom screens or
optimized conditions now simply involves pipetting StockOptions reagents from convenient kits.
StockOptions Salt contains 49 unique salts, preformulated at convenient stock concentrations, each in 10
ml volumes, all in a single kit with a 5" x 9" footprint that saves precious lab space. It is designed to help
researchers improve the speed, accuracy, precision, and quality of the formulation of crystallization opti-
mization solutions. Researchers can use the individual StockOptions reagents to formulate custom screen
solutions or accurately reproduce standard screen solutions from Hampton Research crystallization kits.
All one needs to do is select the reagent and pipet.
The convenience also reduces the chance of errors. Preformulated stocks remove calculation, measure-
ment, and formulation errors. No more second guessing how the reagent was formulated, what specific
chemical was used, when it was made, or how to precisely reproduce that reagent when it is gone and
more reagent is required for additional setups.
StockOptions are cost-effective, time saving reagents. When a StockOptions kit is purchased, one is
using reagents as preformulated stocks in reasonable volumes. You buy only the reagents you need, not
a large container of raw material that may sit out on shelves for years to come. Waste is further reduced
since there is no chance for formulation or measuring errors. When you need larger volumes, Hampton
Research offers individual, preformulated, sterile filtered Optimize crystallization reagents which include
salts, polymers, organics solvents, and buffers. Each of the 49 salts offered in StockOptions Salt is available
individually as an Optimize crystallization grade reagent. Optimize, Custom Shop, StockOptions, and all
Hampton Research kits are synergistic research tools.
StockOptions kits also lower the costs associated with making crystallization reagents since there is no
need to purchase sterile filters, filtration devices, or pre-sterilized storage containers. Cost savings are also
extended to labor since time can now be better utilized for sample production and purification or setting
crystallization experiments.
References
1. Purification, crystallization and preliminary crystallographic characterization of the caspase-recruitment domain of human Nod1. T. Srimathi, S. L. Robbins,
R. L. Dubas, J.-H. Seo and Y. C. Park. Acta Cryst. (2007). F63, 21-23.
s T o C k o P T I o n s

S A L T
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 62
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Order Information
Each StockOptions Salt kit contains 49 unique reagents.
Cat. No. Name Description Price
HR2-245 StockOptions Salt 10 ml, tube format $285.00
stockoptions salt kit formulation
1.0 M Ammonium acetate
5.0 M Ammonium chloride
2.5 M Ammonium phosphate monobasic
10.0 M Ammonium fluoride
10.0 M Ammonium formate
2.5 M Ammonium citrate dibasic
3.5 M Ammonium phosphate dibasic
10.0 M Ammonium nitrate
3.5 M Ammonium sulfate
2.0 M Ammonium tartrate dibasic
1.0 M Calcium acetate hydrate
2.0 M Calcium chloride dihydrate
5.0 M Lithium acetate dihydrate
10.0 M Lithium chloride
1.5 M Lithium citrate tribasic tetrahydrate
8.0 M Lithium nitrate
2.0 M Lithium sulfate monohydrate
1.0 M Magnesium acetate tetrahydrate
2.0 M Magnesium chloride hexahydrate
1.0 M Magnesium formate dihydrate
3.0 M Magnesium nitrate hexahydrate
2.5 M Magnesium sulfate hydrate
4.0 M Nickel(II) chloride hexahydrate
5.0 M Potassium acetate
4.0 M Potassium chloride
2.5 M Potassium citrate tribasic monohydrate
1.5 M Potassium phosphate monobasic
6.0 M Potassium fluoride
14.0 M Potassium formate
3.0 M Potassium phosphate dibasic
3.0 M Potassium nitrate
1.5 M Potassium sodium tartrate tetrahydrate
0.5 M Potassium sulfate
8.0 M Potassium thiocyanate
3.0 M Sodium acetate trihydrate
5.0 M Sodium chloride
1.6 M Sodium citrate tribasic dihydrate
5.0 M Sodium phosphate monobasic monohydrate
0.8 M Sodium fluoride
7.0 M Sodium formate
1.0 M Sodium phosphate dibasic dihydrate
3.4 M Sodium malonate pH 7.0
7.0 M Sodium nitrate
1.0 M Sodium sulfate decahydrate
1.5 M Sodium tartrate dibasic dihydrate
8.0 M Sodium thiocyanate
1.2 M Succinic acid pH 7.0
1.0 M Zinc acetate dihydrate
2.0 M Zinc sulfate heptahydrate
Reagent
Number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
Reagent
n
Crystallization grade buffer stocks for crystal
screening, optimization, & production
n
Titrated, preformulated, & ready-to-use buffers
n
pH 2.2 - 11.0
n
Easy transition from screening to optimization
and production
n
Synergistic with Hampton Research screens,
kits, & reagents
n
Concentrated stocks
n
Sterile filtered
a p p l i c a t i o n
f e a t u r e s
d e s c r i p t i o n
StockOptions reagent kits are reagent tool boxes for the
macromolecular crystal grower. They offer precisely for-
mulated, high quality crystallization grade reagent stocks
in convenient, cost-effective kits. The chemicals utilized
in these kits are the same crystallization grade, ultra-high
purity chemicals utilized in the Hampton Research kits such
as Crystal Screen and Crystal Screen 2. StockOptions
reagents are carefully formulated under strict quality stan-
dards to ensure reliable performance and lot-to-lot con-
sistency.
StockOptions pH is a convenient and complete kit with buffers pHed from 2.2 to 11.0 in increments of
0.2 pH units. Each reagent is available in convenient concentration, making crystal setups quick and easy.
Gone is the tedious task of finding and sourcing reagents, as well as costly and time-consuming reagent
formulation.
The generation of custom screens or optimized conditions now simply involves pipetting StockOptions
pH reagents from one convenient kit. This portfolio of buffers between pH 2.2 and 11, along with wide
arrays of salts, polymers, and organic solvents also stimulates creativity since all of the tools are readily and
conveniently available. All one needs to do is select the reagent and pipet.
The convenience also reduces the chance of errors. Preformulated stocks remove calculation, measure-
ment, and formulation errors. No more second guessing how the reagent was formulated, what specific
chemical was used, when it was made, or how to precisely reproduce that reagent when it is gone and
more reagent is required for additional setups.
StockOptions pH is cost-effective. When a StockOptions kit is purchased, one is using reagents as prefor-
mulated stocks in reasonable volumes. You buy only the reagents you need, not a large container of raw
material that may sit out on shelves for years to come. When you need larger volumes, Hampton Research
offers individual, preformulated, sterile filtered Optimize crystallization reagents which include salts,
polymers, organics solvents, and buffers. Optimize, Custom Shop, StockOptions, and all Hampton
Research kits are synergistic research tools.
References
1. Structure of the ribosomal interacting GTPase YjeQ from the enterobacterial species Salmonella typhimurium. D. K. Stammers et al. Acta Cryst. (2007).
F63, 922928
2. Increasing the size of microcrystals by fine sampling of pH limits. A. McPherson. J. Appl. Cryst. (1995). 28, 362-365.
63
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Order Information
Each StockOptions pH kit contains 45 unique reagents. To order individual reagents, use Custom
Shop

catalog number listed below. Refer to page 37 for further details.


Cat. No. Name Description Price
HR2-241 StockOptions pH 10 ml, tube format $275.00
HR2-941-** StockOptions pH Custom Shop 185 ml
Reagents 1-39 $139.00
Reagents 40-45 $151.00
** = reagent number 1-45
s T o C k o P T I o n s


P
H
sToCkoPTIons PH kIT ForMulaTIon
pH
2.2
2.4
2.6
2.8
3.0
3.2
3.4
3.6
3.8
4.0
4.2
4.4
4.6
4.8
5.0
5.2
5.4
5.6
5.8
6.0
6.2
6.4
6.6
6.8
7.0
7.2
7.4
7.6
7.8
8.0
8.2
8.4
8.6
8.8
9.0
9.2
9.4
9.6
9.8
10.0
10.2
10.4
10.6
10.8
11.0
Reagent
1.0 M Citric acid
1.0 M Citric acid
1.0 M Citric acid
1.0 M Citric acid
1.0 M Citric acid
1.0 M Citric acid
1.0 M Citric acid
1.0 M Citric acid
1.0 M Citric acid
1.0 M Sodium acetate trihydrate
1.0 M Sodium acetate trihydrate
1.0 M Sodium acetate trihydrate
1.0 M Sodium acetate trihydrate
1.0 M Sodium acetate trihydrate
1.0 M Sodium citrate tribasic dihydrate
1.0 M Sodium citrate tribasic dihydrate
1.0 M Sodium citrate tribasic dihydrate
1.0 M Sodium citrate tribasic dihydrate
1.0 M Sodium citrate tribasic dihydrate
1.0 M Sodium cacodylate trihydrate
1.0 M Sodium cacodylate trihydrate
1.0 M Sodium cacodylate trihydrate
1.0 M Sodium cacodylate trihydrate
1.0 M Sodium cacodylate trihydrate
1.0 M HEPES sodium
1.0 M HEPES sodium
1.0 M HEPES sodium
1.0 M HEPES sodium
1.0 M HEPES sodium
1.0 M TRIS hydrochloride
1.0 M TRIS hydrochloride
1.0 M TRIS hydrochloride
1.0 M TRIS hydrochloride
1.0 M TRIS hydrochloride
0.5 M CAPSO
0.5 M CAPSO
0.5 M CAPSO
0.5 M CAPSO
0.5 M CAPSO
0.5 M CAPS
0.5 M CAPS
0.5 M CAPS
0.5 M CAPS
0.5 M CAPS
0.5 M CAPS
Titrant
Sodium hydroxide
Sodium hydroxide
Sodium hydroxide
Sodium hydroxide
Sodium hydroxide
Sodium hydroxide
Sodium hydroxide
Sodium hydroxide
Sodium hydroxide
Hydrochloric acid
Hydrochloric acid
Hydrochloric acid
Hydrochloric acid
Hydrochloric acid
Hydrochloric acid
Hydrochloric acid
Hydrochloric acid
Hydrochloric acid
Hydrochloric acid
Hydrochloric acid
Hydrochloric acid
Hydrochloric acid
Hydrochloric acid
Hydrochloric acid
Hydrochloric acid
Hydrochloric acid
Hydrochloric acid
Hydrochloric acid
Hydrochloric acid
Sodium hydroxide
Sodium hydroxide
Sodium hydroxide
Sodium hydroxide
Sodium hydroxide
Sodium hydroxide
Sodium hydroxide
Sodium hydroxide
Sodium hydroxide
Sodium hydroxide
Sodium hydroxide
Sodium hydroxide
Sodium hydroxide
Sodium hydroxide
Sodium hydroxide
Sodium hydroxide
Reagent
Number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
n
Focused, titrated buffer stocks for crystal
screening, optimization & production
n
pH range 2.2 - 6.5 (44 unique reagents)
n
0.1 pH unit increments for fine tuning pH
n
Compatible with Hampton Research screens,
kits & reagents
n
1.0 M concentrated stocks
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 64
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StockOptions Sodium Acetate buffer kit is a preformulated, sterile filtered set of titrated buffer stocks.
The reagents are supplied as 1.0 M stock solutions in 10 ml volumes. Each StockOptions Sodium
Acetate buffer reagent is carefully titrated using Hydrochloric acid. The kit is comprised of 21 unique
reagents covering the pH range of 3.6 to 5.6 in 0.1 pH unit increments.
References
1. Crystal structures of reduced, oxidized, and mutated human thioredoxins: evidence for a regulatory homodimer. Weichsel, A; Gasdaska, JR; Powis,
G; Montfort, WR. Structure 4, 735- 751, 1996.
2. Increasing the size of microcrystals by fine sampling of pH limits. A. McPherson. J. Appl. Cryst. (1995). 28, 362-365.
s t o c k o p t i o ns s o d i um a c e t a t e
O
ONa H
3
C
3H
2
O
pH 3.6 - 5.6
Order Information
Each StockOptions Citric Acid kit contains 44 unique reagents. To order individual reagents, use
Custom Shop

catalog number listed below. Refer to page 37 for further details.


Cat. No. Name Description Price
HR2-104 StockOptions Citric Acid 10 ml, tube format $195.00
HR2-904-** StockOptions Citric Acid Custom Shop 185 ml $144.00
** = reagent number 1-44
Order Information
Each StockOptions Sodium Acetate kit contains 21 unique reagents. To order individual reagents,
use Custom Shop

catalog number listed below. Refer to page 37 for further details.


Cat. No. Name Description Price
HR2-233 StockOptions Sodium Acetate 10 ml, tube format $195.00
HR2-933-** StockOptions Sodium Acetate Custom Shop 185 ml $150.00
** = reagent number 1-21
StockOptions Citric Acid buffer kit is a preformulated, sterile fltered set of titrated buffer stocks.
The reagents are supplied as 1.0 M stock solutions in 10 ml volumes. Each StockOptions Citric Acid
buffer reagent is carefully titrated using Sodium hydroxide. StockOptions Citric Acid is comprised of
44 unique reagents covering the pH range of 2.2 to 6.5 in 0.1 pH unit increments.
References
1. Increasing the size of microcrystals by ne sampling of pH limits. A. McPherson. J. Appl. Cryst. (1995). 28, 362-365.
s t o c k o p t i o ns c i t r i c a c i d
pH 2.2 - 6.5
OH O O
OH O
OH HO
d e s c r i p t i o n
StockOptions Buffer kits are reagent
toolboxes for the macromolecular crystal
grower. StockOptions reagent kits offer
precisely formulated, high quality crystal-
lization grade reagent stocks in convenient,
cost-effective kits. The chemicals utilized in
StockOptions kits are the same crystallization
grade, ultra-high purity chemicals utilized
in the Hampton Research kits. StockOptions
reagents are carefully formulated under strict
quality standards to ensure reliable performance and lot-to-lot consistency.
Preformulated stocks remove calculation, measurement, and formulation errors. No more second
guessing how the reagent was formulated, what specific chemical was used, when it was made, or how
to precisely reproduce that reagent when it is gone and more reagent is required for additional setups.
s T o C k o P T I o n s

B U F F E R
65
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Order Information
Each StockOptions Sodium Citrate kit contains 24 unique reagents. To order individual reagents, use
Custom Shop

catalog number listed below. Refer to page 37 for further details.


Cat. No. Name Description Price
HR2-235 StockOptions Sodium Citrate 10 ml, tube format $195.00
HR2-935-** StockOptions Sodium Citrate Custom Shop 185 ml $173.00
** = reagent number 1-24
StockOptions Sodium Citrate buffer kit is a preformulated, sterile filtered set of titrated buffer stocks.
The reagents are supplied as 1.0 M stock solutions in 10 ml volumes. Each StockOptions Sodium Citrate
buffer reagent is carefully titrated using Hydrochloric acid. The kit is comprised of 24 unique reagents
covering the pH range of 4.2 - 6.5 in 0.1 pH unit increments.
Formulated using Sodium citrate tribasic dihydrate and HCl.
pKa1 = 3.1 pKa2 = 4.8 pKa3 = 5.4
References
1. Increasing the size of microcrystals by fine sampling of pH limits. A. McPherson. J. Appl. Cryst. (1995). 28, 362-365.
s t o c k o p t i o ns s o d i um c i t r a t e b uf f e r
NaO
O
ONa
ONa
HO
H
2
O
H
2
O
O
O
CH
3
3H
2
O As
O
NaO CH
3
OH
S
O
O
O N
H
2
O
pH 4.2 - 6.5
StockOptions Sodium Cacodylate buffer kit is a preformulated, sterile filtered set of titrated buffer
stocks. The reagents are supplied as 1.0 M stock solutions in 10 ml volumes. Each StockOptions
Sodium Cacodylate buffer reagent is carefully titrated using Hydrochloric acid. The kit is comprised of
24 unique reagents covering the pH range of 5.1 to 7.4 in 0.1 pH unit increments.
References
1. Increasing the size of microcrystals by fine sampling of pH limits. A. McPherson. J. Appl. Cryst. (1995). 28, 362-365.
s t o c k o p t i o ns s o d i um c a c o d y l a t e b uf f e r
pH 5.1 - 7.4
StockOptions MES buffer kit is a preformulated, sterile filtered set of titrated buffer stocks. The
reagents are supplied as 1.0 M stock solutions in 10 ml volumes. Each StockOptions MES buffer reagent
is carefully titrated using Sodium hydroxide. The kit is comprised of 20 unique reagents covering the
pH range of 5.2 to 7.1 in 0.1 pH unit increments.
References
1. Characterization, crystallization and preliminary crystallographic analysis of human recombinant cyclooxygenase-2. Marco, SD; Priestle, JP; Grutter, MG;
Wennogle, LP; Boyar, W. Acta Crystallogr D 53, 224- 226, 1997.
2. Increasing the size of microcrystals by ne sampling of pH limits. A. McPherson. J. Appl. Cryst. (1995). 28, 362-365
s t o c k o p t i o ns me s b uf f e r
pH 5.2 - 7.1
Order Information
Each StockOptions Sodium Cacodylate kit contains 24 unique reagents. To order individual
reagents, use Custom Shop

catalog number listed below. Refer to page 37 for further details.


Cat. No. Name Description Price
HR2-239 StockOptions Sodium Cacodylate 10 ml, tube format $250.00
HR2-939-** StockOptions Sodium Cacodylate Custom Shop 185 ml $221.00
** = reagent number 1-24
Order Information
Each StockOptions MES kit contains 20 unique reagents. To order individual reagents, use Custom
Shop

catalog number listed below. Refer to page 37 for further details.


Cat. No. Name Description Price
HR2-243 StockOptions MES 10 ml, tube format $195.00
HR2-943-** StockOptions MES Custom Shop 185 ml $138.00
** = reagent number 1-20
O
O
S ONa HO
N N
pH 6.8 - 8.2
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 66
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StockOptions Bis-Tris buffer kit is a preformulated, sterile filtered set of titrated buffer stocks. The
reagents are supplied as 1.0 M stock solutions in 10 ml volumes. Each StockOptions Bis-Tris buffer
reagent is carefully titrated using Hydrochloric acid. The kid is comprised of 21 unique reagents cover-
ing the pH range of 5.5 to 7.5 in 0.1 pH unit increments.
References
1. Increasing the size of microcrystals by fine sampling of pH limits. A. McPherson. J. Appl. Cryst. (1995). 28, 362-365.
Each kit contains 15 unique 10 ml volumes of 1.0 M HEPES buffer solution titrated to the indicated
pH using NaOH.
References
1. The expression, purification and crystallization of the epsilon subunit of the F1 portion of the ATPase of Escherichia coli. Codd, R; Cox, GB; Guss, JM;
Solomon, RG; Webb, D. J Mol Biol 228, 306- 309, 1992.
2. Increasing the size of microcrystals by fine sampling of pH limits. A. McPherson. J. Appl. Cryst. (1995). 28, 362-365.
s t o c k o p t i o ns b i s - t r i s
s t o c k o p t i o ns he p e s
StockOptions Sodium HEPES buffer kit is a preformulated, sterile filtered set of titrated buffer stocks.
The reagents are supplied as 1.0 M stock solutions in 10 ml volumes. Each StockOptions Sodium HEPES
buffer reagent is carefully titrated using Hydrochloric acid. The kit is comprised of 15 unique reagents
covering the pH range of 6.8 to 8.2 in 0.1 pH unit increments.
References
1. Increasing the size of microcrystals by fine sampling of pH limits. A. McPherson. J. Appl. Cryst. (1995). 28, 362-365.
s t o c k o p t i o ns s o d i um he p e s
s T o C k o P T I o n s

B U F F E R
pH 6.8 - 8.2
pH 5.5 - 7.5
N
OH
OH
HO
HO
HO
S
O
O
OH HO
N N
Order Information
Each StockOptions Bis-Tris kit contains 21 unique reagents. To order individual reagents, use
Custom Shop

catalog number listed below. Refer to page 37 for further details.


Cat. No. Name Description Price
HR2-106 StockOptions Bis-Tris 10 ml, tube format $195.00
HR2-906-** StockOptions Bis-Tris Custom Shop 185 ml $151.00
** = reagent number 1-21
Order Information
Each StockOptions HEPES kit contains 15 unique reagents. To order individual reagents, use
Custom Shop

catalog number listed below. Refer to page 37 for further details.


Cat. No. Name Description Price
HR2-102 StockOptions HEPES 10 ml, tube format $195.00
HR2-902-** StockOptions HEPES Custom Shop 185 ml $172.00
** = reagent number 1-15
Order Information
Each StockOptions Sodium HEPES kit contains 15 unique reagents. To order individual reagents,
use Custom Shop

catalog number listed below. Refer to page 37 for further details.


Cat. No. Name Description Price
HR2-231 StockOptions Sodium HEPES 10 ml, tube format $195.00
HR2-931-** StockOptions Sodium HEPES Custom Shop 185 ml $190.00
** = reagent number 1-15
67
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NH
2
OH
OH HO
NH
2
OH
OH HO HCl
pH 7.0 - 9.0
pH 7.0 - 9.0
StockOptions Tris buffer kit is a preformulated, sterile filtered set of titrated buffer stocks. The reagents
are supplied as 1.0 M stock solutions in 10 ml volumes. Each StockOptions Tris buffer reagent is carefully
titrated using Hydrochloric acid. The kit is comprised of 21 unique reagents covering the pH range of 7.0
to 9.0 in 0.1 pH unit increments.
References
1. Crystallization and Preliminary X-ray Diffraction Analysis of the ArsC Protein from the Escherichia coli Arsenical Resistance Plasmid, R773. deMel,
VSJ; Doyle, MA; Gladysheva, TB; Oden, KL; Martin, PD; Rosen, BP; Edwards, BFP. J Mol Biol 242, 701- 702, 1994.
2. Increasing the size of microcrystals by fine sampling of pH limits. A. McPherson. J. Appl. Cryst. (1995). 28, 362-365.
StockOptions Tris Hydrochloride buffer kit is a preformulated, sterile filtered set of titrated buffer
stocks. The reagents are supplied as 1.0 M stock solutions in 10 ml volumes. Each StockOptions Tris
Hydrochloride buffer reagent is carefully titrated using Sodium hydroxide. The kit is comprised of 21
unique reagents covering the pH range of 7.0 to 9.0 in 0.1 pH unit increments.
References
1. Increasing the size of microcrystals by fine sampling of pH limits. A. McPherson. J. Appl. Cryst. (1995). 28, 362-365.
s t o c k o p t i o ns t r i s
s t o c k o p t i o ns t r i s hy d r o c hl o r i d e
"The Lord of the Ring" - Crystal of bacterial di-haem Cyt c4
grown using the Hampton Research Additive Screen.
Ivana Tomcova, Institute of Physical Biology,
Academy of Science of the Czech Republic.

Order Information
Each StockOptions Tris kit contains 21 unique reagents. To order individual reagents, use Custom
Shop

catalog number listed below. Refer to page 37 for further details.


Cat. No. Name Description Price
HR2-100 StockOptions Tris 10 ml, tube format $195.00
HR2-900-** StockOptions Tris Custom Shop 185 ml $169.00
** = reagent number 1-21
Order Information
Each StockOptions Tris Hydrochloride kit contains 21 unique reagents. To order individual reagents,
use Custom Shop

catalog number listed below. Refer to page 37 for further details.


Cat. No. Name Description Price
HR2-237 StockOptions Tris Hydrochloride 10 ml, tube format $195.00
HR2-937-** StockOptions Tris Hydrochloride Custom Shop 185 ml $139.00
** = reagent number 1-21
As in selecting reagents for crystallization, the selection
of a suitable cryoprotectant involves some trial and error
as well as a screening.
1-15
A suitable cryoprotectant, when
mixed with the crystal and crystallization reagent, will
cool to cryogenic temperature without ice formation and
damage to the crystal. To assay for the proper concentra-
tion of cryoprotectant in the reagent used to grow the
crystal, one can mix the cryoprotectant with the crystal-
lization reagent and employ the desired cooling method.
For example, place the solution in a CryoLoop and
place the CryoLoop in a cryostream. Observe for ice formation either visually or with x-ray diffraction.
Upon cooling, a transparent drop and x-ray diffraction pattern, free of powder diffraction rings or ice
rings indicates success. The appearance of a cloudy drop or ice rings, indicates an inappropriate
cryoprotectant concentration or cryoprotectant. Incrementally increase the concentration and/or com-
position of the cryoprotectant serially 5 to 10% and repeat until the cooled drop remains clear while in
the cryostream. Once a clear drop is achieved in the cryostream, this is typically a good starting point
for cryopreservation of the crystal.
Some crystals can simply be dipped or washed quickly in a simple cryoprotectant such as 30% glycerol
for successful cryopreservation. However, when all else fails, a rational assay of each cryoprotectant
with incremental increases in cryoprotectant concentration, as well as a test of mixtures (i.e. a mixture
of sugars or a sugar mixed with Ethylene glycol) may be required to determine the best cryoprotectant
for a crystal.
Each CryoPro - Cryoprotectant kit contains 36 unique cryoprotectants including organics, oils, polyols,
salts, sugars, and polymers. 35 of the 36 reagents are supplied in 10 ml volumes. A single reagent L-(+)-
2,3-Butanol is supplied in a 0.2 ml aliquot. CryoPro is convenient and cost-effective.
Ready-to-use cryoprotectants are sterile filtered and formulated with ultra-pure water, using high
purity reagents. Many of the individual cryoprotectants are available as a 100 ml or 200 ml Optimize
reagent. Please refer to the CryoPro Formulations pdf file to find the catalog number for the equivalent
Optimize.
References
1. Boutron, P. (1987). Non-equilibrium formation of ice in aqueous solutions: efficiency of polyalcohol solutions for vitrification. In: Pegg, D.E. & Karow,
A.M. Jr. (eds). The biophysics of organ prese
2. Garman, E.F., & Mitchell, E.P. (1996). Glycerol concentrations required for cryoprotection of 50 typical protein crystallization conditions. J. Appl. Cryst. 29,
584-587.
3. Garman, E.F., & Schneider, T.R. (1997). Macromolecular Cryocrystallography. J. Appl. Cryst. 30, 211-237.
4. Hope, H. (1988). Cryocrystallography of biological macromolecules: a generally applicable method. Acta Cryst. B 44, 22-26.
5. Kottke, T., & Stalke, D. (1993). Crystal handling at low temperatures. J. Appl. Cryst. 26, 615-619.
6. Kwong, P.D. Liu, Y. (1999). Use of cryoprotectants in combination with immiscible oils for flash cooling macromolecular crystals. J. Appl. Cryst. 32, 102-105.
7. Mehl, P. (1989). Experimental dissection of devitrification in aqueous solutions in 1,3-butanediol. Cryobiology. 26, 567-568.
8. Parkin, S., & Hope, H. (1998). Macromolecular cryocrystallography: Cooling, mounting, storage and transportation of crystals. J. Appl. Cryst. 31, 945-953.
9. Petcock, J.M., Wang, Y.-F., DuBois, G.C., Harrison, R.W., & Weber, I.T. (2001). Effects of different post-crystallization soaking conditions on the diffraction
of Mtcp1 crystals. Acta Cryst. D57, 763-
10. Petsko, G.A. (1975). J. Mol. Biol. 96, 381-392.
11. Rodgers, D.W. (1994). Cryocrystallography. Structure. 2, 1135-1140. 12. Schneider, T.R. (1997). Cryocrystallography of biological macromolecules.
Acta Physica Polonica A. 91, 739-744.
12. Teng, T.-Y. (1990). J. Appl. Cryst. 23, 387-391.
13. Walker, L.J., Moreno, P.O., Hope, H. (1998). Cryocrystallography: effect of cooling medium on sample cooling rate. J. Appl. Cryst. 31, 954-965.
14. Watenpaugh, K.D. (1991). Curr. Op. Struct. Biol. 1, 1012.
15. Schneider, T.R. (1997), Cryocrystallography of biological macromolecules. Acta Physica Polonica A. 91, 739-744.
n
Cryoprotectant reagent kit
n
36 unique cryoprotectants
n
Includes cryoprotectant tutorial
n
Preformulated, ready-to-use
n
Polyols, organics, oils, polymers, sugars,
& salts
s T o C k o P T I o n s


-
C R Y O P R O

Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 68
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d e s c r i p t i o n
Order Information
Each CryoPro kit contains 36 unique reagents.
Cat. No. Name Description Price
HR2-132 CryoPro 10 ml, tube format $285.00
c ry o p r o
Cryoprotectant
F o r m u l a t i o n s
100% Glycerol
100% Ethylene glycol
100% Polyethylene glycol 200
100% Polyethylene glycol 400
80% v/v Polyethylene glycol 600
60% w/v Polyethylene glycol 4,000
50% w/v Polyvinylpyrrolidone K 15
100% (+/-)-2-Methyl-2,4-pentanediol
6.0 M 1,6-Hexanediol
100% 1,2-Propanediol
100% Paratone-N
100% Paraffin Oil
100% NVH Oil
100% Dimethyl sulfoxide (DMSO)
100% 2-Propanol
100% Ethanol
100% Methanol
70% w/v D-(+)-Sucrose
35% w/v meso-Erythritol
70% w/v Xylitol
15% w/v myo-Inositol
20% w/v D-(+)-Raffinose pentahydrate
50% w/v D-(+)-Trehalose dihydrate
70% w/v D-(+)-Glucose monohydrate
100% 2,3-Butanediol
100% L-(+)-2,3-Butanediol
5.0 M Lithium acetate dihydrate
10.0 M Lithium chloride
4.0 M Lithium formate monohydrate
8.0 M Lithium nitrate
2.0 M Lithium sulfate monohydrate
3.4 M Sodium malonate pH 7.0
3.5 M Magnesium acetate tetrahydrate
5.0 M Sodium chloride
7.0 M Sodium formate
7.0 M Sodium nitrate
Reagent
Number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
Crystals shaped like a dragonfly wing.
Yi-Wei Change, Institute of Molecular Biology,
Academia Sinica, Taiwan.
"Butterfly"-crystal of hydroxynitrile lyase from
Prunus amygdalus grown with the Hampton
Research Grid Screen PEG/LiCl.
Andreas Giessauf at the Institute for Chemie,
Karl-Franzens-Universitt Graz, Austria.
Crystals of the FERM domain of Focal Adhesion Kinase.
The final precipitant was derived from an initial hit obtained
with condition 17 from Hampton Research Crystal Screen.
Derek F.J. Ceccarelli, Samuel Lunenfeld Research Institute,
Mount Sinai Hospital, Toronto, Ontario, Canada.
c r y s t a l l i z a t i o n p l a t e s ,
h a r d w a r e & a c c e s s o r i e s
Crystals of a frameshift promoting RNA pseudoknot from the coronavirus IBV, grown using the Hampton Research Natrix screen.
Simon Pennell, MRC National Institute for Medical Research, United Kingdom.
T A B L E O F C O N T E N T S
72 - 77 2 4 w e l l c r y s t a l l i z a t i o n p l a t e s
78 - 79 4 8 w e l l c r y s t a l l i z a t i o n p l a t e s
79 7 2 w e l l c r y s t a l l i z a t i o n p l a t e s
80 - 90 9 6 w e l l c r y s t a l l i z a t i o n p l a t e s
90 3 8 4 w e l l c r y s t a l l i z a t i o n p l a t e s
91 - 95 c o v e r s l i d e s & r e l a t e d t o o l s
95 g l a s s s i t t i n g d r o p r o d s

96 m i c r o - b r i d g e s

97 9 w e l l g l a s s p l a t e
97 - 98 s e a l a n t s , s e a l i n g g r e a s e & o i l s
99 - 101 s e a l i n g f i l m s , t a p e s , m a t s & c o v e r s
102 - 103 d i a l y s i s b u t t o n s

, m e m b r a n e s & a p p l i c a t o r s
104 g r a n a d a c r y s t a l l i z a t i o n b o x

& c a p i l l a r i e s
P A G E S
2 4 W E L L C R Y S T A L L I Z A T I O N P L A T E S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 72
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d e s c r i p t i o n
C R Y S C H E M

P L A T E
The Cryschem Plate is a 24 well sitting drop plate that is sealed
with clear sealing tape. The plate is supplied with a raised cover so
the plate can also be sealed with vacuum grease and plain glass or
plastic cover slides (22 mm diameter). The stackable, 24 well plates
have distortion-free, flat bottom wells and a concave sitting drop
post in the center of the well for concentric equilibration of the
drop with the reagent. Each plate is individually sealed. The top
of the plate and each of the 24 wells is a single, flat plane for smooth and easy seal application. Well
spacing provides for a large seal area between wells to prevent cross contamination and evaporation.
Approximate size: 15.1 cm x 10.6 cm x 2.2 cm. Approximate well size: 1.6 cm x 1.5 cm. Typical fill
volume: 500 to 1,000 l. Well capacity: 1.5 ml. Maximum drop volume on post: 40 l. Recommended
seal: HR3-511 Crystal Clear Sealing Tape (1.88 inch x 43.7 yard roll, with cutter) or HR4-511 Crystal
Clear Sealing Tape (1.88 inch x 60 yard roll, without cutter).
The Cryschem 24-1 SBS Plate is a smaller, SBS footprint 24 well sitting drop microplate that is sealed
with tape or film. The plate is supplied with a raised cover so the plate can also be sealed with vacuum
grease and plain glass or plastic cover slides (18 mm diameter). The stackable, 24 well plates have
distortion-free, flat bottom wells and a concave sitting drop post in the center of the well for concentric
equilibration of the drop with the reagent. Each plate is individually sealed. The top of the plate and
each of the 24 wells is a single, flat plane for smooth and easy seal application. Well spacing provides
for a large seal area between wells to prevent cross contamination and evaporation. Approximate size:
127.8 mm x 85.5 mm x 14.4 mm. Typical fill volume: 500 to 700 l. Maximum drop volume on post: 12
l. Recommended seal: HR3-609 Crystal Clear Sealing Film (100 pack), HR4-521 ClearSeal Film (100
pack) or HR4-506 Crystal Clear Sealing Tape (3 inch x 55 yard roll).
References
1. Purification, crystallization and preliminary X-ray crystallographic analysis of ST1022, a putative member of the Lrp/AsnC family of transcriptional regulators
isolated from Sulfolobus tokodaii strain 7. Shigeyuki Yokoyama et al. Acta Cryst. (2007). F63, 964966
n
Sitting drop crystallization

n
Heavy atom soaks

n
Seeding
n
Optically clear, concave sitting drop post

n
Smooth, flat top for easy seal
n
Optically clear polystyrene
a p p l i c a t i o n s
f e a t u r e s
m a t e r i a l
Order Information
Cat. No. Name Description Price
HR3-158 Cryschem Plate 24 plate case $95.00
HR3-160 Cryschem Plate 100 plate case $350.00
HR1-002 Cryschem 24-1 SBS Plate 50 plate case $150.00
12 mm 18 mm 22 mm Crystal Clear ClearSeal 1.88" 3" AlumaSeal II
Plate # Wells Cover Slide Cover Slide Cover Slide Sealing Film Film Tape Tape Film
SEALS
24 X
24 X X X
24 X
24 X
24 X
24 X
24 X X X
48 X X X
48 X
96 X X X
96 X X X
96 X X X
96 X X X
96 X X X
96 X
Cryschem
Cryschem 24-1 SBS
VDX
VDXm
Greiner ComboPlate
Linbro

Intelli-Plate 24 Well
Intelli-Plate 48 Well
VDX48
Douglas Instruments
CrystalClear Strips
Corning

Greiner CrystalQuick
Intelli-Plate
MRC/Swissci
MaSTeRBLOCk

A
B
C
D
1 3 5 2 4 6
Top Side
A
B
C
D
1 3 5 2 4 6
Top Side
Top
Side
73
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Cover Slide (or Sealing Tape)
Well of VDX
Crystallization Plate
Reservoir Solution
Dialysis Button
Vacuum
Grease
n
Hanging Drop Crystallization
n
Dialysis Crystallization with Dialysis Buttons
Cover Slide (or Sealing Tape)
Crystallization Droplet
Well of VDX
or Linbro Plate
Reservoir Solution
Micro-Bridge
n
Sitting Drop Crystallization with Micro-Bridges or Glass Rods
d e s c r i p t i o n
The Intelli-Plate 24-4 is a 24 well sitting drop plate for crystallization
screening and optimization. It features 4 reagent wells along the
y-axis (A-D) and 6 reagent wells along the x-axis (1-6). The reagent
reservoir is typically filled with 250 l and is capable of holding
between 200 and 650 l. The 4 drop wells are arranged at the top
of each well and can hold up to 8 l (5 l for hydrophobic reagents
such as MPD).
The Hampton Research ClearSeal Film and Sealing Film Applicator are used to seal the Intelli-Plate
when a sitting drop experiment is performed. The advanced film is a transparent, polyolefin film with
clear, pressure-sensitive, silicone adhesive. The film is pre-cut to perfectly fit 96 well crystallization
plates and is complete with 1 cm wide, perforated end tabs which makes handling and positioning
of the film easy, without fear of fingerprints getting into the view field. The Sealing Film Applicator is
a small, hand-held applicator which should be used to create a consistent film seal across the entire
crystallization plate. The Intelli-Plate can also be sealed using one of the clear sealing tapes supplied
in a roll format.
The height of all Intelli-Plate 24-4 is 0.560 inches. The Art Robbins Instruments catalog number for this
plate is 102-0004-00.
n
Sitting drop crystallization

n
4 drop wells per reservoir

n
Optically clear polystyrene
n
alphanumeric labeled drop wells visible under
microscope
a p p l i c a t i o n
f e a t u r e s
I N T E L L I - P L A T E

2 4 - 4 W E L L
Order Information
Cat. No. Name Description Price
HR3-114 Intelli-Plate 24-4 well 40 plate case $200.00
Proteins crystals.
Kasumi Kobayashi, Taiji Nakae and Hiroyuki Akama.
The Kitasato Institute, Kanagawa, Japan.

Intelli-Plate 24-4 well
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
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2 4 W E L L C R Y S T A L L I Z A T I O N P L A T E S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 74
d e s c r i p t i o n
V D X

P L A T E
24 well crystallization plate for hanging drop or sitting drop vapor
diffusion crystallization (when used with Micro-Bridges or Glass
Rods), or Dialysis crystallization (when used with Dialysis Buttons).
Stackable, optically clear plastic 24 well plates with raised covers (to
allow room for cover slides) and flat bottoms for exceptional optics.
Raised, wide rings around each reservoir (well) minimize cross
contamination and allow each well to be individually sealed with
22 mm diameter circle or square cover slides. Plates are individu-
ally wrapped and supplied without sealant. 15.0 cm x 10.8 cm plate
footprint is convenient for manual pipetting and well access while
at the same time compatible with some automated liquid handling
systems. Approximate dimensions: 15.0 cm x 10.6 cm x 2.2 cm.
Approximate well size: 1.7 cm x 1.6 cm. Typical well volume: 500 to
1,000 l. Well capacity: 3.5 ml.
n
Hanging drop crystallization

n
Sitting drop crystallization with Micro-Bridges


or Glass Sitting Drop Rods


n
Dialysis crystallization with Dialysis Buttons

n
Compatible with 22 mm diameter square
and circle cover slides

n
24 well plate with raised cover
n
extremely versatile and cost-effective
crystallization platform
n
Optically clear plastic
a p p l i c a t i o n s
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR3-142 VDX Plate 24 plate case $65.00
HR3-140 VDX Plate 100 plate case $250.00
d e s c r i p t i o n
V D X

P L A T E W I T H S E A L A N T
24 well crystallization plate for hanging drop or sitting drop vapor
diffusion crystallization (when used with Micro-Bridges or Glass
Rods), or dialysis crystallization (when used with Dialysis Buttons).
Stackable, optically clear plastic 24 well plates with raised covers (to
allow room for cover slides) and flat bottoms for exceptional optics.
Raised, wide rings around each reservoir (well) minimize cross
contamination and allow each well to be individually sealed with 22
mm diameter circle or square cover slides. Plates are individually
wrapped and supplied with applied sealant. 15.0 cm x 10.8 cm plate
footprint is convenient for manual pipetting and well access while
at the same time compatible with some automated liquid handling
systems. Approximate dimensions: 15.0 cm x 10.6 cm x 2.2 cm.
Approximate well size: 1.7 cm x 1.6 cm. Typical well volume: 500 to
1,000 l. Well capacity: 3.5 ml.
n
Hanging drop crystallization

n
Sitting drop crystallization with Micro-Bridges

or Glass Sitting Drop Rods


n
Dialysis crystallization with Dialysis Buttons
n
Sealant applied to 24 wells, ready to seal
with cover slides

n
Compatible with 22 mm diameter circle
and square cover slides
n
24 well plate with raised cover
n
Optically clear plastic
n
extremely versatile and cost-effective
crystallization platform
a p p l i c a t i o n s
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR3-172 VDX Plate with sealant 24 plate case $110.00
HR3-170 VDX Plate with sealant 100 plate case $400.00
75
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d e s c r i p t i o n
d e s c r i p t i o n
24 well plate for hanging drop vapor diffusion crystallization with a
microplate footprint. Stackable, optically clear plastic, 24 well plates
with raised covers (to allow room for cover slides) and flat bottoms
for exceptional optics. Raised, wide rings around each reservoir
(well) minimize cross contamination and allow each well to be indi-
vidually sealed with 18 mm diameter circle cover slides. Plates are
individually wrapped and supplied without sealant. The microplate
footprint is space-saving and compatible with automated liquid
handling systems. Approximate dimensions: 128 mm x 85 mm. Approximate well ID: 14.4 mm. Typical
well volume: 100 to 500 l.
24 well plate with sealant for hanging drop vapor diffusion
crystallization with a 128 mm x 85 mm microplate footprint.
Stackable, optically clear plastic, 24 well plates with raised
covers (to allow room for cover slides) and flat bottoms for
exceptional optics. Raised, wide rings around each reservoir
(well) minimize cross contamination and allow each well to
be individually sealed with 18 mm diameter circle cover slides.
Plates are individually wrapped and supplied with applied seal-
ant. The microplate footprint is space-saving and compatible
with automated liquid handling systems. Approximate dimen-
sions: 128 mm x 85 mm. Approximate well ID: 14.4 mm. Typical
well volume: 100 to 500 l.
n
Hanging drop crystallization
n
24 well plate with raised cover

n
Compatible with 18 mm diameter circle cover
slides
n
Optically clear plastic
n
128 mm x 85 mm microplate footprint
n
Hanging drop crystallization
n
24 well plate with raised cover

n
Sealant applied to 24 wells, ready to seal
with cover slides
n
Compatible with 18 mm diameter circle cover
slides
n
Optically clear plastic
n
128 mm x 85 mm microplate footprint
a p p l i c a t i o n
a p p l i c a t i o n
f e a t u r e s
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR3-108 VDXm Plate without sealant 50 plate case $130.00
Hanging Drop Crystallization
Order Information
Cat. No. Name Description Price
HR3-306 VDXm Plate with sealant 50 plate case $210.00
Crystals of a nucleoporin complex.
James Partridge, Massachusetts Institute of Technology, USA.

V D X M

P L A T E
V D X M

P L A T E W I T H S E A L A N T
2 4 W E L L C R Y S T A L L I Z A T I O N P L A T E S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
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76
d e s c r i p t i o n
The Greiner ComboPlate is a 24 well plate with a raised cover in an
SBS microplate footprint. The plate, supplied without sealant, can
be sealed with an 18 mm diameter cover slide and sealant for hang-
ing drop crystallization. When used with the Greiner CrystalBridge,
the ComboPlate can be used for sitting drop crystallization and
sealed with sealing film/tape or 18 mm diameter cover slide and
sealant. Dimensions: 127.76 mm x 85.48 mm x 18.8 mm. Well
depth: 16.28 mm. 3.3 ml well capacity. Typical fill volume: 0.5 - 1.0
ml. Supplied with cover. The Greiner CrystalBridge is a polystyrene
sitting drop pedestal with a concave drop well (45 l capacity) and
is designed specifically for the ComboPlate.
n
Hanging and sitting drop crystallization

n
24 reagent wells

n
Sitting drop when used with CrystalBridge
n
ComboPlate has flat, raised rings about
each well to minimize spills and cross
contamination
n
ComboPlate seals with 18 mm glass or
plastic cover slides
n
Slightly raised protection lid
n
Polystyrene
a p p l i c a t i o n
f e a t u r e s
m a t e r i a l s
G R E I N E R C O M B O P L A T E

A N D C R Y S T A L B R I D G E

Order Information
Cat. No. Name Description Price
HR3-200 24 Well ComboPlate with cover, Greiner 662150 24 plate case $61.00
HR3-150 CrystalBridge for ComboPlate, Greiner 662145 100 pack $78.00
HR3-154 CrystalBridge for ComboPlate, Greiner 662145 400 pack $276.00
d e s c r i p t i o n
The Linbro 24 well plate with cover is made of clear, rigid, polysty-
rene and used primarily for hanging drop vapor diffusion crystal-
lization. Sitting drop vapor diffusion may be performed when used
with Micro-Bridges or Glass Sitting Drop Rods. Plates supplied
without sealant. Compatible with 22 mm diameter circle and
square cover slides. Each plate is individually wrapped and sterile.
Wide, raised rings around each reservoir minimizes cross contami-
nation and allows each individual well to be sealed using vacuum
grease, DC 7 Release Compound or immersion oil and a siliconized or plain cover slide. Cover is not
raised but can be raised by placing wax or mounting clay in each of the four corners of the cover. The
stackable plates have distortion-free, flat-bottom wells of fine clarity. Wells are identified by lettered
rows A through D and numbered columns 1 through 6. Approximate dimensions: 15.0 cm x 10.8 cm
x 2.2 cm. Approximate well size: 1.7 cm x 1.6 cm. Typical well volume: 700 to 1,000 l. Individual well
capacity: 3.5 ml
References
1. Expression, purification and crystallization of 2-oxo-hept-4-ene-1,7-dioate hydratase (HpcG) from Escherichia coli C. T. Adachi, A. Izumi, D. Rea, S.-Y. Park,
J. R. H. Tame and D. I. Roper. Acta Cryst. (2006). F62, 1010-1012.
n
Hanging drop crystallization

n
Sitting drop crystallization with Micro-Bridges

or Glass Sitting Drop Rods

n
Dialysis crystallization with Dialysis Buttons


n
24 well plate with cover
n
Compatible with 22 mm diameter circle and
square cover slides
n
Optically clear plastic
a p p l i c a t i o n s
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR3-110 Linbro Plate 50 plate case $302.00
HR3-112 Linbro Plate 100 plate case $592.00
L I N B R O

P L A T E
77
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d e s c r i p t i o n
The Modular VDX Plate is a standard 24 well VDX Plate with
individually removable wells. The modular format, consisting
of a VDX frame and removable wells creates a very flexible
plate format for screening and optimization of crystallization
experiments.
Preliminary crystallization screens can be set in the Modular
VDX Plate. Once the results are scored, the wells can be
removed and organized into separate frames for optimization
and observation. Preliminary crystallization screens typically
evaluate a diverse array of reagents, reagent concentrations, pH values, and other variables that can
influence sample solubility and crystallization. These crystallization variables, combined in a single well
can have a unique effect on the sample solubility and are useful for screening preliminary crystallization
conditions. The crystallization screen typically delivers a wide range of solubility results, presented in
some wells as clear drops, others as precipitate, phase separation, or crystals. Clear drops can indicate
the relative supersaturation of the sample in the drop as too low for crystallization, while precipitate
can indicate the relative supersaturation as too high. If the sample has a temperature dependent solu-
bility, the temperature of the experiment can be raised or lowered, and the effect of this change can
be evaluated based upon the appearance of the drop. For example, a clear drop at room temperature
moved to 10C might result in a change in sample solubility, hence precipitate, phase separation,
or even a crystal. A precipitate at room temperature, moved to 30C may again result in a change in
sample solubility, hence precipitate, phase separation, or a crystal. When a standard crystallization
plate with fixed wells is used for screening, the decision to move the entire plate to a different tem-
perature can be complicated since all the wells in the plate must be moved together. This may be less
than ideal when crystals appears in one of those wells, or there is a mix of clear, precipitated, and phase
sep drops where each well requires a different course of action. Each solubility result or class (clear,
precipitate, phase sep, etc.) might require a unique temperature evaluation. With the Modular VDX
Plate, individual wells can be removed from the frame, organized by crystallization score or solubility
into a separate frame or frames, and the effect of temperature evaluated on a more discrete basis. For
example, clear drops can be removed from the screen frame, complete with their respective equili-
brated reservoir solution, placed into a separate frame and that newly created module can be incubated
at a lower temperature. Drops with precipitate can be removed from the screen frame, organized
into a new module and be incubated at an elevated temperature to see if an increase in temperature
improves sample solubility and promotes crystallization.
Beyond screening, the Modular VDX Plate also offers enhanced plate flexibility and customization
during optimization of preliminary crystallization experiments. Optimization experiments can be per-
formed in Modular VDX frames, utilizing removable wells to reorganize the plate as the optimization
proceeds. More promising conditions can be grouped into a single frame, reducing the actual utilized
active screen space required. Less promising experiments can be archived and stored into other
Modular VDX frames.
Identification of the Modular VDX Plates is simple. Frames and well sides can be labeled using a variety
of color coded Tough-Tag labels.
The Modular VDX Plates are also handy for the organization of drops and wells containing crystals.
Wells containing crystals suitable for diffraction analysis can be combined, stored, and carefully trans-
ported in modules, avoiding the extra baggage of wells containing clear drops and precipitate.
Just like the original VDX Plate, the Modular VDX Plate is compatible with Micro-Bridges

, Glass Sitting
Drop Rods, as well as 22 mm circle and square cover slides.
References
1. A modular plate for the optimization of crystallization experiments. J. Appl. Cryst. (2002) 35, 509-510.
n
Sitting, hanging drop, and dialysis
crystallization

n
Modular wells for temperature studies
1

n
Removable wells

n
24 well plate with raised cover
n
Wells available with or without sealant
n
Use with 22 mm diameter circle and square
cover slides
n
Optically clear plastic
a p p l i c a t i o n s
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR3-198 Modular VDX Frames 24 frames with covers $57.00
HR3-196 Modular VDX Wells no sealant, 576 wells $70.00
HR3-204 Modular VDX Well with sealant, 96 wells $25.00
V D X

P L A T E ( M O D U L A R )
4 8 W E L L C R Y S T A L L I Z A T I O N P L A T E S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 78
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d e s c r i p t i o n
The Intelli-Plate 48-2 is a 48 well sitting drop plate for otpi-
mization. The Intelli-Plate 48-2 features 6 reagent wells along
the y-axis (A-F) and 8 reagent wells along the x-axis (1-8). The
reagent reservoir is typically filled with 200 l and is capable of
holding between 100 and 500 l. The optimization drop wells
are arranged at the top of each well. The left sample drop well
holds between 4 l or less while the elongated sample well holds
20 l or less.
The IntelliPlate 48-3 is a 48 well sitting drop plate for screening.
The Intelli-Plate 48-3 features 6 reagent wells along the y-axis
(A-F) and 8 reagent wells along the x-axis (1-8). The reagent
reservoir is typically filled with 200 l and is capable of holding
between 100 and 500 l. The screening drop wells are arranged
at the top of each well. The three sample drop wells each hold
between 4 l or less.
The Hampton Research ClearSeal Film and Sealing Film
Applicator are used to seal the Intelli-Plate when a sitting drop
experiment is performed. The advanced film is a transparent,
polyolefin film with clear, pressure-sensitive, silicone adhesive.
The film is pre-cut to perfectly fit 96 well crystallization plates and
is complete with 1 cm wide, perforated end tabs which makes
handling and positioning of the film easy, without fear of fingerprints getting into the view field. The
Sealing Film Applicator is a small, hand-held applicator which should be used to create a consistent
film seal across the entire crystallization plate. The Intelli-Plate can also be sealed using one of the clear
sealing tapes supplied in a roll format.
The height of all Intelli-Plates is 0.560 inches.
n
Sitting Drop Crystallization

n
SBS Footprint

n
Intelli-Plate 48-2 Drop Volume:
20 l & 4 l
Reservoir: 500 l
n
Intelli-Plate 48-3 Drop Volume: 4 l
Reservoir: 500 l
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR8-150 Intelli-Plate 48-2 well 10 plate case $85.00
HR8-152 Intelli-Plate 48-2 well 40 plate case $300.00
HR8-154 Intelli-Plate 48-3 well 10 plate case $85.00
HR8-156 Intelli-Plate 48-3 well 40 plate case $300.00
Individual Side Well View
Drop Support
Reservoir
Drop Support
Reservoir
Individual Top Well View Intelli-Plate & Intelli-Plate 48-2
Drop Support
Individual Top Well View Intelli-Plate 48-3
Reservoir
HR8-152
HR8-156
I N T E L L I - P L A T E 4 8 - 2 A N D 4 8 - 3
Top Side Well
Top Side Well
Top Side Well
79
Hanging Drop Crystallization
d e s c r i p t i o n
d e s c r i p t i o n
48 well plate with sealant for hanging drop vapor diffusion crys-
tallization with a microplate footprint. Stackable, optically clear
plastic, 48 well plates with raised covers (to allow room for cover
slides)and flat bottoms for exceptional optics. Raised, wide rings
around each reservoir (well) minimize cross contamination and
allow each well to be individually sealed with 12 mm diameter
circle cover slides. Plates are individually wrapped and supplied
with applied sealant. The microplate footprint is space-saving and
compatible with automated liquid handling systems. Approximate
dimensions: 128 mm x 85 mm. Approximate well ID: 9 mm. Typical
well volume: 100 to 300 l.
The 72 Well Microbatch Plate consists of a 6 by 12 well layout.
Paraffin, Silicon, or Als Oil is poured into the trough area of the
plate, submerging all 72 wells under oil. Sample and reagent are
then pipetted into each of the wells. The plate is available as a
plasma-treated, hydrophilic version for screening or an untreated,
hydrophobic version for optimization. The hydrophilic version
gives better liquid handling with small volumes, while the hydro-
phobic version reduces crystal nucleation and helps to prevent the
crystals from sticking to the plastic. Plate dimensions: 83.3 mm x 58.0 mm x 10.0 mm. Conical well with
11 l drop volume. Flat bottom well diameter: 1.3 mm. Supplied with cover.
n
Hanging drop crystallization
n
Sealant applied to 48 wells, ready to seal
with cover slide

n
Compatible with 12 mm diameter circle cover
slides
n
Optically clear plastic
n
128 mm x 85 mm microplate footprint
n
Microbatch crystallization
n
Conical, flat bottom wells

n
Hydrophobic or hydrophilic versions
n
Compact size
n
Supplied with cover
n
Polystyrene
a p p l i c a t i o n
a p p l i c a t i o n
f e a t u r e s
f e a t u r e s
m a t e r i a l
Order Information
Cat. No. Name Description Price
HR3-120 72 Well Microbatch Plate, Greiner 654180 treated, hydrophylic - $10.00
10 plate case
HR3-122 72 Well Microbatch Plate, Greiner 654180 treated, hydrophylic - $106.00
100 plate case
HR3-086 72 Well Microbatch Plate, Greiner 654102 untreated, hydrophobic - $10.00
10 plate case
HR3-087 72 Well Microbatch Plate, Greiner 654102 untreated, hydrophobic - $106.00
100 plate case
7 2 W E L L C R Y S T A L L I Z A T I O N P L A T E S
V D X 4 8

P L A T E W I T H S E A L A N T
Order Information
Cat. No. Name Description Price
HR3-275 VDX48 Plate with sealant 50 plate case $335.00
M I C R O B A T C H 7 2 W E L L P L A T E ( G R E I N E R )
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9 6 W E L L C R Y S T A L L I Z A T I O N P L A T E S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 80
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CrystalClear Strips 96 well sitting drop plates are suitable for
both automatic and manual crystallization. They are particularly
useful for screening, since they feature a space-saving microplate
footprint. Sample and reagent drops (50 nl to 4 l) are dispensed
on a shelf on one side of each well. 50 to 100 l of crystallization
reagent is placed in the well. The strip is sealed with clear sealing
tape. Losses by evaporation though the body of the strip and the
tape are around 0.25% of the reservoir per day.
CrystalClear D (Douglas Instruments CCLEAR-D/1-10)
The CrystalClear D Strips, with indent (HR3-128) have a single
depression for the sample and reagent drop on the shelf above
each reservoir. This prevents hydrophobic reagents from running
off the shelf into the reservoir. This version should be used for
reagents and screens that contain low molecular weight alcohols
and detergents. The spacing between strips is 9 mm (the regular
SBS spacing). However, the spacing between sample chambers
along a D strip is only 8.45 mm. Therefore, if you want to use a
multichannel pipette to fill either the reservoirs or the sample wells,
use a 6 or 12-channel pipette.
CrystalClear P (Douglas Instruments CCLEAR-P/1-10)
The CrystalClear P Strips, without indent (HR3-162) have a small, raised, circular platform on the shelf
where samples are placed. This provides improved viewing but is not suitable for solutions containing
hydrophobic reagents such as low molecular weight alcohols and detergents. However, drops below
0.2 l (200 nl) containing alcohol (and other hydrophobic reagents) will generally work with all plates.
The spacing between strips is 9 mm (the regular SBS spacing). However, the spacing between sample
chambers along a P strip is only 8.45 mm. Therefore, if you want to use a multichannel pipette to fill
either the reservoirs or the sample wells, use a 6 or 12-channel pipette.
CrystalClear Duo (Douglas Instruments CCLEAR-DUO/1-10)
The CrystalClear Duo Strips is a 96 well sitting drop vapor diffusion plate. The plate features SBS 9
mm spacing in both directions, which makes the plate suitable for use with all dispensing robots and
in all observation systems. The plate offers low volume reagent wells for vapor diffusion with two
depressions on the shelf for samples. A plate consists of a single frame and twelve strips, snapped into
position, secured in the frame. Each strip contains eight wells. Each of the eight wells contains a single
reagent well and two sample drop wells. Sample drop wells are flat. Frame is color coded green.
CrystalClear Strips are packaged as twelve removable 8 well strips per frame (for a total of 96 wells per
frame) with ten frames (plates) per case. CrystalClear Strips are sealed using clear sealing tape or film.
Approximate plate size: 12.8 cm x 8.6 cm.
n
Sitting drop vapor diffusion crystallization
n
96 well sitting drop plate

n
Concave or flat drop area

n
Modular frame and well design

n
One or two sample drop wells per
reagent well
n
Polystyrene

a p p l i c a t i o n
f e a t u r e s
m a t e r i a l
Order Information
Cat. No. Name Description Price
HR3-128 CrystalClear D Strips, with indent 10 plate case $169.00
HR3-162 CrystalClear P Strips, without indent 10 plate case $169.00
DI-043 CrystalClear Duo Strips 10 plate case $145.00
HR3-128 CrystalClear D Strips, with indent HR3-162 CrystalClear P Strips, without indent DI-043 CrystalClear Duo Strips
HR3-162
HR3-128
C R Y S T A L C L E A R S T R I P S

( D O U G L A S I N S T R U M E N T S )
81
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Corning

CrystalEX 96 Well Crystallization plates are designed


for sitting drop vapor diffusion, high throughput protein crystal-
lization. The plates feature 96 protein wells corresponding to 96
reagent wells. The maximum reagent well volume is 210 l with
a typical working volume of 25 to 150 l. The maximum sample
well volume is 10 l for the conical bottom and 7 l for the flat
bottom. The flat bottom (1.5 mm diameter) sample wells provide
better crystal viewing for automated imaging systems. The well
surfaces are treated to be hydrophilic for improved drop mixing.
Both versions are compatible with 8 and 12 channel manual pipetters, automated liquid handling work
stations, and imaging systems. Both versions are manufactured from an advanced polymer with high
resistance to commonly used protein crystallization reagents. The advanced polymer material provides
for low-background polarization and high optical clarity so protein crystals may be viewed under polar-
ized light with minimal background interference. Low water absorption of the polymer prevents loss of
protein drop volume. The plates can be sealed using clear sealing tape or film.
n
Sitting drop crystallization
n
96 well sitting drop plate
n
Protein well shape is either conical bottom
(3773) or flat bottom (3785)

n
Compatible with 8 & 12 channel pipetters

n
advanced optically clear polymer

a p p l i c a t i o n
f e a t u r e s
m a t e r i a l
Order Information
Cat. No. Name Description Price
HR3-273 CrystalEX 96 Well, Conical Bottom, Corning 3773 10 plate case $110.00
HR3-271 CrystalEX 96 Well, Conical Bottom, Corning 3773 50 plate case $500.00
HR3-113 CrystalEX 96 Well, Flat Bottom, Corning 3785 10 plate case $110.00
HR3-115 CrystalEX 96 Well, Flat Bottom, Corning 3785 50 plate case $500.00
Individual Top Well View
Drop Support
Reservoir
Concave, Round Bottom
Drop support
Reservoir
Conical, Flat Bottom
Corning 3773 Corning 3785
Protein well shape Conical bottom Flat bottom
Protein well volume 10 l 7 l
Protein well dimensions (top/bottom) 3 mm 3 mm/1.5 mm
Protein well depth 3.1 mm 3.1 mm
Number of reagent/protein wells 96/96 96/96
Reagent well volume 210 l 210 l
Recommended reagent working volume 25-150 l 25-150 l
C R Y S T A L E X

( C O R N I N G )
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 82
9 6 W E L L C R Y S T A L L I Z A T I O N P L A T E S
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The second generation of Corning

96 Well, sitting drop


format plates are built to SBS specifications, making them
well suited for high throughput crystallization and are fully
compatible with robotic equipment. The plates are avail-
able in several different configurations with varying drop
well shapes, plate materials, and number of drop wells
per reagent well. The basic plate design is one reagent
well flanked by one or three drop wells, with SBS stan-
dard spacing between the centers of adjacent well clusters. One may choose a plate with small (1 l),
medium (2 l), or large (4 l) drop well volumes. The choice of round, flat, or conical flat well shapes
are available. The PZero polymer provides for zero background polarization and is non-birefringent.
PZero plates are not treated. The COC polymer offers high chemical resistance. Both types of plastic
feature improved transparency. The reservoir numbers are embossed on each individual well for easy
identification. Drop well locations conform to SBS standards for robotic handling. The low-volume
reagent well saves on reagent costs. The plates can be sealed using clear sealing tape or film.
n
Sitting drop crystallization
n
96 well sitting drop plate
n
Round, flat, and conical flat bottom drop well
shapes

n
1, 2 or 4 microliter drop wells
n
1, 3 or 5 drop wells per reservoir
n
Hydrophilicity treated or untreated
n
PZero or COC plate material
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR8-135 Corning 3556 4 l round drop well, 1 drop well, COC, $105.00
untreated - 10 plate case
HR8-134 Corning 3556 4 l round drop well, 1 drop well, COC, $490.00
untreated - 50 plate case
HR8-137 Corning 3551 4 l conical flat drop well, 1 drop well, COC, $105.00
treated - 10 plate case
HR8-136 Corning 3551 4 l conical flat drop well, 1 drop well, COC, $490.00
treated - 50 plate case
HR8-139 Corning 3552 2 l round drop well, 3 drop well, PZero $105.00
- 10 plate case
HR8-138 Corning 3552 2 l round drop well, 3 drop well, PZero $490.00
- 50 plate case
HR8-141 Corning 3553 2 l conical flat drop well, 3 drop well, PZero $105.00
- 10 plate case
HR8-140 Corning 3553 2 l conical flat drop well, 3 drop well, PZero $490.00
- 50 plate case
HR8-147 Corning 3550 1 l conical flat drop well, 3 drop well, PZero $105.00
- 10 plate case
HR8-146 Corning 3550 1 l conical flat drop well, 3 drop well, PZero $490.00
- 50 plate case
Corning 3554 CrystalEx
HR8-143/42
Corning 3552 CrystalEx
HR8-139/38
Corning 3556 CrystalEx
HR8-135/34
Corning 3555 CrystalEx
HR8-145/44
Corning 3550 CrystalEx
HR8-146/47
Corning 3557 CrystalEx
HR8-160/158
Corning 3551 CrystalEx
HR8-137/36
Corning 3553 CrystalEx
HR8-141/40
Corning 3554 CrystalEx
HR8-143/42
Corning 3552 CrystalEx
HR8-139/38
Corning 3556 CrystalEx
HR8-135/34
Corning 3555 CrystalEx
HR8-145/44
Corning 3550 CrystalEx
HR8-146/47
Corning 3557 CrystalEx
HR8-160/158
Corning 3551 CrystalEx
HR8-137/36
Corning 3553 CrystalEx
HR8-141/40
Corning 3554 CrystalEx
HR8-143/42
Corning 3552 CrystalEx
HR8-139/38
Corning 3556 CrystalEx
HR8-135/34
Corning 3555 CrystalEx
HR8-145/44
Corning 3550 CrystalEx
HR8-146/47
Corning 3557 CrystalEx
HR8-160/158
Corning 3551 CrystalEx
HR8-137/36
Corning 3553 CrystalEx
HR8-141/40
Corning 3554 CrystalEx
HR8-143/42
Corning 3552 CrystalEx
HR8-139/38
Corning 3556 CrystalEx
HR8-135/34
Corning 3555 CrystalEx
HR8-145/44
Corning 3550 CrystalEx
HR8-146/47
Corning 3557 CrystalEx
HR8-160/158
Corning 3551 CrystalEx
HR8-137/36
Corning 3553 CrystalEx
HR8-141/40
C R Y S T A L E X

S E C O N D G E N E R A T I O N ( C O R N I N G )
Corning 3554 CrystalEx
HR8-143/42
Corning 3552 CrystalEx
HR8-139/38
Corning 3556 CrystalEx
HR8-135/34
Corning 3555 CrystalEx
HR8-145/44
Corning 3550 CrystalEx
HR8-146/47
Corning 3557 CrystalEx
HR8-160/158
Corning 3551 CrystalEx
HR8-137/36
Corning 3553 CrystalEx
HR8-141/40
Top Side Well
83
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Sitting drop vapor diffusion crystallization
n
Hanging drop vapor diffusion crystallization
with CrystalDrop

Lid
n
96 well sitting drop plate
n
Round or flat bottom drop wells

n
Hanging drop with CrystalDrop

Lid

n
Plastic
a p p l i c a t i o n
f e a t u r e s
m a t e r i a l
Order Information
Cat. No. Name Description Price
HR3-192 CrystalQuick 96 Well, 4 l square drop well, $130.00
Greiner 609101 3 drop well - 10 plate case
HR3-190 CrystalQuick 96 Well, 4 l square drop well, $490.00
Greiner 609101 3 drop well - 40 plate case
HR3-094G CrystalQuick Plus 96 Well, 4 l square drop well, 3 drop well, $139.00
Greiner 609830 LBR, hydrophobic - 10 plate case
HR3-095G CrystalQuick Plus 96 Well, 4 l square drop well, 3 drop well, $528.00
Greiner 609830 LBR, hydrophobic - 40 plate case
HR8-148 CrystalQuick Plus 96 Well, 4 l square drop well, 3 drop well, $130.00
Greiner 609130 hydrophobic - 10 plate case
HR8-149 CrystalQuick Plus 96 Well, 4 l square drop well, 3 drop well, $490.00
Greiner 609130 hydrophobic - 40 plate case
HR3-088 CrystalQuick 96 Well, 4 l square drop well, 3 drop well, $139.00
Greiner 609801 LBR, hydrophobic - 10 plate case
HR3-089 CrystalQuick 96 Well, 4 l square drop well, 3 drop well, $528.00
Greiner 609801 LBR, hydrophobic - 40 plate case
HR3-302 CrystalQuick 96 Well, 4 l square drop well, $230.00
Greiner 609171 1 drop well, low profile - 20 plate case
HR3-304 CrystalQuick 96 Well, 4 l square drop well, 1 drop well, $830.00
Greiner 609171 low profile - 80 plate case
HR3-092G CrystalQuick Plus 96 Well, 4 l square drop well, 1 drop well, $266.00
Greiner 609180 hydrophobic, low profile - 20 plate case
HR3-093G CrystalQuick Plus 96 Well, 4 l square drop well, 1 drop well, $1,011.00
Greiner 609180 hydrophobic, low profile - 80 plate case
HR3-284 CrystalQuick 96 Well, 4 l square drop well, 1 drop well, $255.00
Greiner 609871 LBR, low profile - 20 plate case
HR3-285 CrystalQuick 96 Well, 4 l square drop well, 1 drop well, $880.00
Greiner 609871 LBR, low profile - 80 plate case
HR3-283 CrystalQuick 96 Well, 2 l round drop well, $130.00
Greiner 609120 3 drop well - 10 plate case
HR3-281 CrystalQuick 96 Well, 2 l round drop well, $490.00
Greiner 609120 3 drop well - 40 plate case
HR3-090 CrystalQuick 96 Well, 2 l round drop well, $139.00
Greiner 609820 3 drop well, LBR - 10 plate case
HR3-091 CrystalQuick 96 Well, 2 l round drop well, $528.00
Greiner 609820 3 drop well, LBR - 40 plate case
HR3-096 CrystalDrop Lid, Greiner 609150 2 flat wells, ungreased - 10 lid case $54.00
HR3-097 CrystalDrop Lid, Greiner 609150 2 flat wells, ungreased - 40 lid case $201.00
HR3-137 Greased CrystalDrop Lid, 2 flat wells, greased - 10 lid case $233.00
Greiner 609050
HR3-138 Greased CrystalDrop Lid, 2 flat wells, greased - 40 lid case $850.00
Greiner 609050
The Greiner CrystalQuick 96 well sitting drop format plates are
built to SBS specifications, making them well suited for high
throughput crystallization and are fully compatible with robotic
equipment. The low volume reagent well saves on reagent costs.
The plates are available in round or square drop well shapes,
polystyrene, LBR and hydrophobic plate materials, and a varying
number of drop wells per reagent well. The CrystalQuick is avail-
able in a cyclic polyolefin low birefringence (LBR) material for
polarized crystal imaging, exceptional transparency, high chemical
resistance, and low water absorption. The low profile CrystalQuick version reduces the amount of
space required for storage. In combination with the CrystalDrop Lid, the CrystalQuick plates enable
simultaneous experiments using both sitting drop and hanging drop methodologies. The plates can be
sealed using clear sealing tape or film.
C R Y S T A L Q U I C K

9 6 W E L L S I T T I N G D R O P P L A T E ( G R E I N E R )
Top Side
Top Side
Top Side
Top
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1 2 3 4 5 6 7 8 9 10 11 12
Top Side
9 6 W E L L C R Y S T A L L I Z A T I O N P L A T E S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 84
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The Intelli-Plate 96-2 Original crystallization plate is an optically
clear plate for sitting drop vapor diffusion crystallization. It has
been built to SBS specifications with 8 vertical wells versus 12
horizontal wells. This plate features two locations for sample per
reservoir. The sample drop locations are located along the left side
of the reagent reservoir, along the Y-axis of the plate. The sample
wells are concave depressions on the ledge above the adjacent, flat
bottom reagent reservoir. One sample well is located above the
second sample well. The top sample well can hold 10 l or less
of sample. The lower sample well can hold 4 l or less of sample. The reagent reservoir is typically
filled with 70 l of reagent and is capable of holding up to 140 l of reagent. The sidewalls separating
adjacent wells or reservoirs are 0.9 and 1.0 mm thick in order to offer a larger area for sealing the plate
and separation of the reservoirs. The vertical wells are labeled A-H along the left side of the plate and
the horizontal wells are labeled 1-12 along the top of the plate. The Intelli-Plate 96-2 is quite rigid,
with virtually no torsional flex and is designed for either manual or automated pipetting. All wells have
standard 9 mm spacing to conform to SBS standards. The Art Robbins Instruments catalog number
for this plate is 102-0001-00.
The Intelli-Plate 96-3 well crystallization plate is an optically clear plate for sitting drop vapor diffusion
crystallization. It has been built to SBS specifications with 8 vertical wells versus 12 horizontal wells.
This plate features three identical locations for sample per reservoir. The sample drop locations are
located along the left side of the reagent reservoir, along the Y-axis of the plate. The sample wells are
concave depressions on the ledge above the adjacent, flat bottom reagent reservoir. Each identical
well features a round bottom for easy crystal harvesting. Each drop well can hold up to 1 l of sample.
The reagent reservoir is typically filled with 70 l of reagent and is capable of holding up to 140 l of
reagent. The sidewalls separating adjacent wells or reservoirs are 0.9 and 1.0 mm thick in order to offer
a larger area for sealing the plate and separation of the reservoirs. The vertical wells are labeled A-H
along the left side of the plate and the horizontal wells are labeled 1-12 along the top of the plate. All
wells have standard 9 mm spacing to conform to SBS standards.
The Intelli-Plate 96 Flat Shelf crystallization plate is an optically clear plate for sitting drop vapor dif-
fusion crystallization. It has been built to SBS specifications with 8 vertical wells versus 12 horizontal
wells. This plate features a single flat shelf for sample drops. The sample drop shelves are located along
the left side of the reagent reservoir, along the Y-axis of the plate. The reagent reservoir is typically
filled with 100 l of reagent and is capable of holding up to 300 l of reagent. Maximum drop volume
is 10 l for the upper sample well, & 4 l for the lower sample well. The sidewalls separating adjacent
wells or reservoirs are 0.9 and 1.0 mm thick in order to offer a larger area for sealing the plate and
separation of the reservoirs. The vertical wells are labeled A-H along the left side of the plate and the
horizontal wells are labeled 1-12 along the top of the plate. The Intelli-Plate 96 Flat Shelf is quite rigid,
with virtually no torsional flex and is designed for either manual or automated pipetting. All wells have
standard 9 mm spacing to conform to SBS standards.
The Intelli-Plate 96-2 LVR is a Low Volume Reservoir version of the original Intelli-Plate. The Art
Robbins Instruments catalog number for this plate is 102-0001-00. The plates can be sealed using clear
sealing tape or film. The height of all Intelli-Plates is 0.560 inches.
n
Sitting drop crystallization
n
96 reservoir wells with 2 or 3 corresponding
sample wells

n
96 well Intelli-Plate is compatible with 8 and
12 channel pipetters for manual set up

n
Footprint and well spacing meet microplate
industry standards for automation

n
Intelli-Plate 96-2 well plate features a
maximum fill volume of 140 l for the
reservoir well, 10 l for the upper sample
well, & 4 l for the lower sample well

n
Compatible with robotic equipment for
automation
n
Intelli-Plate Flat Shelf 96 well plate features
a maximum fill volume of 300 l for the reser-
voir well and a flat shelf for the sample drop
n
Intelli-Plate 96-3 plate features a maximum
fill volume of 140 l for the reservoir well,
1 l for the sample well
n
Custom bar coding available - please inquire
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR3-297 Intelli-Plate 96-2 Original 10 plate case $90.00
HR3-299 Intelli-Plate 96-2 Original 40 plate case $330.00
HR3-183 Intelli-Plate 96-3 well 10 plate case $90.00
HR3-185 Intelli-Plate 96-3 well 40 plate case $330.00
HR8-171 Intelli-Plate 96 Flat Shelf 10 plate case $90.00
HR8-172 Intelli-Plate 96 Flat Shelf 40 plate case $330.00
HR3-143 Intelli-Plate 96-2 LVR 10 plate case $90.00
HR3-145 Intelli-Plate 96-2 LVR 40 plate case $330.00
Intelli-Plate
TM
96-3 well Intelli-Plate
TM
96-2 well
Intelli-Plate 96-2 well Intelli-Plate 96-3 well
I N T E L L I - P L A T E

( A R T R O B B I N S I N S T R U M E N T S )
Top Side
Top Side
Top Side
Top Side
Top Side
MRC 2 Well Crystallization Plate
85
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The MRC 2 Well Crystallization Plate manufacted by Swissci is a 2
drop chamber, 96 well crystallization plate for sitting drop vapor
diffusion. This plate is sometimes referred to as the Innovaplate
SD-2 crystallography plate.
The plate was developed at the MRC Laboratory of Molecular
Biology (Cambridge, United Kingdom) in collaboration with Dr.
Jan Lwe. Designed to meet the stringent requirements specified
by crystallographers, and made of proprietary new materials that
facilitate enhanced imaging, the MRC Crystallization Plate provides
easier access and removal of crystals, improved mixing of reagent
and sample, and reduced sample and reagent volumes.
Easy Crystal Retrieval
Raised wide wells make the crystal mounting especially easy.
Easy Viewing
The wells are a wide conical shape and have a lens effect for perfect illumination. The micro-numbering
ensures you will never get lost again when using the microscope. The optically superior polymer is UV
transmissible and may be used to differentiate between salt and protein crystals.
Better Sealing
Wide partition walls between the wells give plenty of area for sealing with tape or film. No central
bending occurs in this very robust structure.
Wide Range of Volumes
Typical volumes are 50 to 100 l of reservoir and 100 nl to 5 l drop size. The 192 optical wells offer
twice the number of experiments of experimental constructions.
SBS Standard
The plate is designed to the 96 well SBS standard for all common holders and external alphanumeric
identification. The MRC Crystallization Plate is suitable for centrifugation.
The plates can be sealed using clear sealing tape or film.
Innovaplate is a trademark of Innovadyne Technologies, Inc.
n
Sitting drop crystallization
n
Lens effect drop wells for enhanced optics

n
Drop volume: 100 nl to 5 l

n
Micro-numbering alongside drop volumes

n
Reservoir volume: 50 to 100 l

n
SBS format
n
Rigid plate structure
n
Drop well allows easier crystal harvesting
n
Wide partition walls between wells improve
sealing
n
Developed in conjunction with the MRC
Laboratory of Molecular Biology in
Cambridge, United kingdom
n
Two drop wells per reservoir
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR3-082 MRC 2 Well Crystallization Plate 10 plate case $130.00
HR3-083 MRC 2 Well Crystallization Plate 40 plate case $490.00
M R C 2 W E L L C R Y S T A L L I Z A T I O N P L A T E ( S W I S S C I )
Protein crystal.
Bebiana Moura,
Institute for Molecular and Cell Biology (IBMC) at Oporto, Portugal.

A
B
A
B
1
1
2
2
MRC 2 Well Crystallization Plate
9 6 W E L L C R Y S T A L L I Z A T I O N P L A T E S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 86
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The MRC 3 Well Crystallization Plate is presented in a 96-well plate
format that offers unique properties which make it ideal for both
nanoliter crystallization screening and microliter optimization alike.
Made from optically superior polymer (UVP) and with a new design
of the wells, the plate allows easy crystal viewing and retrieval.
Easy Crystal Retrieval
Raised wide wells make the crystal mounting especially easy.
Easy Viewing
The wells are a wide conical shape and have a lens effect for perfect
illumination. The micro-numbering ensures you will never get lost
again (visible by microscope). The optically superior polymer is UV
transmissible and may be used to differentiate between salt and
protein crystals. Grown crystals are easy to identify and to remove
from well due to a low-binding polymer. Plate with 3 wells for each
sample, better growing security with triplicates or the ability to use
well two and well three as mixing stations. Wells fill without micro-droplets jumping out due to static
effects. The profile allows for easier storage. Low volume buffer well enables savings on reagents.
Better Sealing
Wide partition walls between the wells give plenty of area for good sealing with tape. No central bend-
ing occurs in this very robust structure.
Wide Range of Volumes
Typical volumes are 50 l of reservior and 100 nl to 5 l drop size. The 288 optical wells offer three
times the number of experimental constructions.
SBS Standard
The plates are designed to the 96-well SBS standard for all common holders and external numbering
(A-H, 1-12) with corner location make the plate easy to use in a robotic sampler. The 3 well plate is
suitable for centrifugation.
The plates can be sealed using clear sealing tape or film.
n
Sitting drop crystallization
n
Lens effect drop wells for enhanced optics

n
Drop volume: 100 nL to 5 l

n
Micro-numbering alongside drop volumes

n
Reservoir volume: 50 l

n
SBS format
n
Drop well allows easier crystal harvesting
n
Three drop wells per reservoir
n
Low profile for easier storage
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR3-123 MRC 3 Well Crystallization Plate 10 plate case $125.00
HR3-125 MRC 3 Well Crystallization Plate 40 plate case $470.00
M R C 3 W E L L C R Y S T A L L I Z A T I O N P L A T E ( S W I S S C I )
MRC 3 Well Crystallization Plate
3 circular drop wells and 1 square reservoir
d e s c r i p t i o n
The Greiner 780261 MASTERBLOCK is a 96 Deep Well
polypropylene block (plate). Each well has a fill volume of
1.2 ml. The MASTERBLOCK is useful for holding crystal-
lization screen reagents when using liquid handling auto-
mation or multi channel pipets. Each plate is sterile and
packaged individually. Round bottom well for complete
reagent recovery. ClearChoice virgin polypropylene
resin (autoclavable to 121C). SBS recommended dimen-
sions. Alphanumerically coded wells with chimney-style, round well top.
Seal with AlumaSeal II, Crystal Clear Sealing Film, Cap Mats, or Robolid. Plate Lid is useful to cover the
opened MASTERBLOCK when not in immediate use to minimize evaporation. MASTERBLOCK is also
great for thermal sealing due to the raised chimney about each well.
n
96 Deep Well plate for crystallization screen
reagents


n
Sterile, individually sealed plates
n
Polypropylene
n
Seal with alumaSeal II, Crystal Clear Sealing
Film, Cap Mats, or Robolid
n
SBS format
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR3-105 MASTERBLOCK 96 Deep Well polypropylene plate 50 plate case $210.00
M A S T E R B L O C K

9 6 D E E P W E L L P L A T E ( G R E I N E R )
MRC 3 Well Crystallization Plate
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The MRC Under Oil 96 Well Crystallization Plate is designed for
microbatch crystallization.
The plate was developed at the MRC Laboratory of Molecular
Biology (Cambridge, United Kingdom) in collaboration with Dr.
Jan Lwe and colleagues.
It is a result of many years of experience in successful robotic high-
throughput crystallization and combines many of the features not
earlier available to the crystallographer.
The new MRC Under Oil 96 Well Crystallization Plate is designed for
microbatch crystallization using paraffin, silicon oil, or a mixture of
the two. Following the initial experiment, evaporation of the drop
through the oil allows for second crystallization stage, enabling
further crystal growth as a consequence of concentration. This is
different from other experiments in that the conditions are then extreme in nature and permit new
conditions to arise.
The new MRC Under Oil 96 Well Crystallization Plate offers unique properties that make it ideal for
both nanoliter crystallization screening and microliter optimization alike. Made from an optically supe-
rior polymer and with a new design of the wells, the plate allows easy crystal viewing and retrieval.
The advantages of the new MRC Under Oil 96 Well Crystallization Plate:
Easy Crystal Retrieval
Raised wide wells make the crystal mounting especially easy
Easy Viewing
The wells are wide conical and have a polished surface on both sides important for perfect illumina-
tion. The micro numbering ensures that you will never get lost again (visible by microscope). The
optically superior polymer is even UV transmissible and may be used to differentiate between salt
and crystals.
Better Sealing
Wide partition walls between the wells give plenty of area for good sealing with tape for the initial
experiments of microbatch. No central bending occurs in this very robust structure.
Recommended Volumes
Typical volumes validated for these plates are 20 l of oil with a shot through sample delivery of 100
to 500 nl. The 20 l volume of the individual wells gives the user a wide range of macromolecular
crystallization possibilities.
SBS Standard
The plates are designed to the 96 well SBS standard for all common holders ad external numbering
(A - H, 1 - 12) with corner location that make the plate easy to use in a robotic sampler. The plate can
also be centrifuged for better results. The unique MRC Under Oil 96 well Crystallization plate offers a
new way of microbatch crystallography. The 96 wells are optically perfect, designed to observe crystals
under a microscope.
Unique Polymer
The proprietary polymer is optically perfect - ultra low binding and guaranteed to have central drop
location in the well. There are no static effects and thus micro-droplet jumping is avoided.
d e s c r i p t i o n
n
Microbatch crystallization

n
easy crystal retrieval
n
easy viewing
n
Drop Volume 100 to 500 nl
n
Oil Volume 20 l
n
SBS Standard
n
Ultra low binding polymer, no static
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR3-102 MRC Under Oil Crystallization Plate 10 plate case $120.00
HR3-104 MRC Under Oil Crystallization Plate 40 plate case $432.00
M R C U N D E R O I L 9 6 W E L L C R Y S T A L L I Z A T I O N P L A T E ( S W I S S C I )
A
1
A
2
B B
1 2
MRC Under Oil
Crystallization Plate
MRC Under Oil Crystallization Plate
9 6 W E L L C R Y S T A L L I Z A T I O N P L A T E S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 88
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d e s c r i p t i o n
The Greiner 96 Well Imp@ct Plate for microbatch crystallization
has conical, flat bottom wells that promote central location of the
sample drop and ease crystal harvesting. The plate has a flat, opti-
cally clear bottom. A raised perimeter wall surrounds the plate to
contain Paraffin Oil, Als Oil, or Silicon Oil. The entire inner area
of the plate or each individual sample well may be filled with oil.
The plate features a double rim reservoir to hold water or reagent
to control and manipulate vapor diffusion from the microbatch
drops. Plate dimensions: 127.76 mm x 85.48 mm x 14.4 mm. Conical flat bottom well with 8 l drop
volume. Flat bottom well diameter: 1.33 mm.
Greiner 673170 plate is clear. Greiner 673096 plate is black with clear drop area.
Plates supplied without cover. Plate Lids HR3-084 or HR3-085 (Greiner 656190) are available sepa-
rately.
The Greiner 673101 96 Well Imp@ct Plate has a low profile format
with individual conical shaped sidewalls and a smooth, flat bot-
tom for enhanced microscopic investigation of crystals. Conical
well shape avoids spreading of the sample and reagent droplet
away from the center of the well. Typical well capacity for Paraffin,
Silicon, or Als Oil is 10 to 20 l per well. Vertical wells are labeled
A-H. Horizontal wells are labeled 1-12. Stackable with supplied
cover. Plate Dimensions: 127.76 mm x 85.48 mm x 8.0 mm. Conical
well with 19 l drop volume. Flat bottom well diameter: 2 mm.
n
Microbatch crystallization

n
Conical, flat bottom wells
n
Clear and black Clear

n
Double rim reservoir
n
Polystyrene
n
Microbatch crystallization

n
Conical, flat bottom wells
n
Supplied with cover
n
Microplate footprint
n
Polystyrene
a p p l i c a t i o n
a p p l i c a t i o n
f e a t u r e s
f e a t u r e s
m a t e r i a l
m a t e r i a l
Order Information
Cat. No. Name Description Price
HR3-098 96 Well Imp@ct Plate, Greiner 673170 with reservoir, no cover - 10 plate case $118.00
HR3-099 96 Well Imp@ct Plate, Greiner 673170 with reservoir, no cover - 40 plate case $444.00
HR3-100 96 Well Imp@ct Plate, Greiner 673096 with reservoir, no cover, black Clear $135.00
- 10 plate case
HR3-101 96 Well Imp@ct Plate, Greiner 673096 with reservoir, no cover, black Clear $482.00
- 40 plate case
HR3-084 Plate Lid, Greiner 656190 10 lid case $10.00
HR3-085 Plate Lid, Greiner 656190 40 lid case $35.00
Order Information
Cat. No. Name Description Price
HR3-295 96 Well Imp@ct Plate, Greiner 673101 with cover, without reservoir $98.00
- 10 plate case
HR3-293 96 Well Imp@ct Plate, Greiner 673101 with cover, without reservoir $350.00
- 40 plate case
I M P @ C T

P L A T E W I T H R E S E R V O I R ( G R E I N E R )
I M P @ C T P L A T E W I T H O U T R E S E R V O I R ( G R E I N E R )
89
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The 96 Well Microbatch Plate is an 8 by 12 well microplate with
cover. Paraffin, Silicon, or Als Oil is dispensed into each individual
well. Sample and reagent are then pipetted into the wells below
the oil. Individual well bottoms are U shaped to center the drop
under oil. Oil can be rapidly (between 3 to 20 seconds) and neatly
dispensed into the plate using V&P Scientific VP195B Multi-Spense
or V&P Scientific VP195B1-96. After sample and reagent are dis-
pensed under the oil using an 8 channel and single channel pipet,
the plates are then centrifuged at very low speed for 5 to 10 minutes to mix and center the drops. The
covered plates can be stored between 0-37C and viewed free of condensation as the covers can be
removed for viewing without drying the drops when using the microbatch method.
n
Microbatch crystallization

n
96 concave bottom wells
n
Microplate footprint
n
Supplied with cover
n
Polystyrene
a p p l i c a t i o n
f e a t u r e s
m a t e r i a l
Order Information
Cat. No. Name Description Price
HR3-265 96 Well Microbatch Plate, with cover 10 plate case $50.00
HR3-267 96 Well Microbatch Plate, with cover 50 plate case $235.00
M I C R O B A T C H 9 6 W E L L P L A T E
d e s c r i p t i o n
The Douglas Instruments Vapor Batch Plate is designed for
both microbatch and sitting drop vapor diffusion crystal-
lization. It has 96 wells in the central region of the plate and
several reservoirs around the outside of the plate. Oil is placed
in the central region followed by sample and reagent for a
microbatch experiment. When the outside reservoirs are filled
with reagent, they can be used to preserve microbatch crys-
tals by preventing the drops from drying out. The reservoirs
can also be used for common dehydrant, sitting drop vapor
diffusion experiments where up to 96 wells are equilibrated against a single reagent or dehydrant.
Preliminary experiments suggest this method may find more hits in screening experiments than the
conventional method of using the same solution in the reservoir and the drop. Wells are individually
labeled. The Vapor Batch Plate is available as a plasma-treated hydrophilic version for screening or an
untreated, hydrophobic version for optimization. The hydrophilic version gives better liquid handling
with small volumes, while the hydrophobic version reduces crystal nucleation and helps to prevent
the crystals from sticking to the plastic. Vapor Batch Plate Holders which provide a Linbro

, VDX,
or SBS footprint are available on a special order basis. Approximate plate dimensions: 81 mm x 55 mm
x 20 mm. Drop well depth: 1.8 mm. Typical drop volume: 1 to 9 l. Maximum drop volume: 20 l.
Recommended oil fill: 2.5 ml. Recommended reagent/dehydrant volume: 8 ml.
n
Sitting drop and microbatch crystallization
n
anaerobic crystallization

n
96 conical, flat bottom wells
n
Supplied with cover
n
Microbatch and vapor diffusion from the
same plate
n
Compact size
n
Hydrophobic or hydrophilic versions
n
Polystyrene
a p p l i c a t i o n s
f e a t u r e s
m a t e r i a l
Order Information
Cat. No. Name Description Price
DI-038 Vapor Batch Plate, treated, hydrophilic 10 plate case $66.00
DI-040 Vapor Batch Plate, treated, hydrophilic 80 plate case $475.00
DI-039 Vapor Batch Plate, untreated, hydrophobic 10 plate case $66.00
DI-041 Vapor Batch Plate, untreated, hydrophobic 80 plate case $475.00
V A P O R B A T C H 9 6 W E L L P L A T E ( D O U G L A S I N S T R U M E N T S )
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 90
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d e s c r i p t i o n
The Multichannel Pipetter Basin is a polypropylene trough
that allows for rapid reproduction of similar reagents or oils
throughout an entire 96 Well crystallization plate. Basin comes
with cover.
n
Pipet oil reservoir for microbatch
crystallization
n
Reagent reservoir for crystallization reagents
when setting Silver Bullets screens

n
Basin with cover
n
Compatible with 8 & 12-channel pipets
n
Polypropylene
a p p l i c a t i o n s
f e a t u r e s
m a t e r i a l
M U L T I C H A N N E L P I P E T T E R B A S I N
Order Information
Cat. No. Name Description Price
HR3-269 Multichannel Pipetter Basin each $7.00
9 6 W E L L C R Y S T A L L I Z A T I O N P L A T E S
3 8 4 W E L L C R Y S T A L L I Z A T I O N P L A T E S
d e s c r i p t i o n
The Corning

CrystalEX is designed for full automation in crys-


tal screening and built to meet industry standards for 384 well
microplate footprint and well locations. Features 192 reservoir
wells with a 105 l volume and 192 corresponding protein wells
with a maximum volume of 4 l. Typical reagent well volumes are
50 l and sample well volumes are 1 l. The reagent wells and sam-
ple wells are positioned to be compatible with multihead dispens-
ing equipment (up to 96 well heads). The plate is manufactured
from an advanced polymer with high resistance to commonly
used solvents, including acetone, acetic acid, butanone, ethanol,
iso-propanol, methanol, DMSO, nitric acid (65%), sulfuric acid
(40%), hydrochloric acid (36%), and ammonia solution (33%). The
advanced polymer features low background polarization and high optical clarity which allows crystals
to be viewed under polarized light with minimal background interference. The low water absorption of
the polymer prevents loss of protein drop volume. The plates can be sealed using Crystal Clear Sealing
Tape or ClearSeal Film. The plate is not treated.
References
1. Expanding screening space through the use of alternative reservoirs in vapor-diffusion experiments. Janet Newman. Acta Cryst. (2005). D61, 490-493.
n
Sitting drop crystallization

n
192 sample wells & 192 reagent wells
n
Flat bottom sample well
n
Low background polarization
n
SBS footprint
n
advanced polymer
n
Use the CrystaleX 384 plate to screen four
different reservoir solutions against the same
crystallization solution to expand screening
space (Newman 2005).
a p p l i c a t i o n
f e a t u r e s
m a t e r i a l
m e t h o d o l o g i e s
Order Information
Cat. No. Name Description Price
HR8-056 CrystalEX 384 Well Flat Bottom Plate 10 plate case $210.00
HR8-058 CrystalEX 384 Well Flat Bottom Plate 50 plate case $875.00
C R Y S T A L E X

3 8 4 W E L L F L A T B O T T O M P L A T E ( C O R N I N G )
Reservoir
Individual side well view
Reservoir
Individual top well view
Drop Support
Drop Support
Individual side well view Individual top well view
91
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C O V E R S L I D E S & R E L A T E D T O O L S
d e s c r i p t i o n
Siliconized glass cover slides allow a droplet to be suspended in
a position which provides near optimal conditions for vapor dif-
fusion with the surrounding reservoir solution. These siliconized
glass cover slides provide a consistent, high quality finish for
crystallization experiments. The hydrophobic surface produces a
drop which stands well and does not flatten on the glass. The
siliconized surface prevents the adhesion of crystals and precipitate
onto the glass surface. Use vacuum grease, DC Release Compound,
or immersion oil to seal cover slide to plate. Available in 12, 18 or
22 mm diameter circles and 22 mm diameter squares. Available in 0.22 mm or 0.96 mm glass thickness.
The 0.96 mm thick slides are virtually unbreakable during crystallization handling.
n
Hanging, sitting or sandwich drop
crystallization
n
Siliconized, hydrophobic glass surface
n
12 mm, 18 mm, & 22 mm glass diameter
n
0.22 mm & 0.96 mm glass thickness
n
Circle & square
a p p l i c a t i o n s
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR3-278T Siliconized circle cover slides for Tecan robot 0.5 oz pack (~240 slides) $40.00
HR3-280T Siliconized circle cover slides for Tecan robot 5 oz case (~2,400 slides) $380.00
HR3-277 12 x 0.22 mm Siliconized circle cover slides 0.5 oz pack (~240 slides) $40.00
HR3-279 12 x 0.22 mm Siliconized circle cover slides 5 oz case (~2,400 slides) $380.00
HR8-088 12 x 0.96 mm Thick Siliconized circle cover slides 1 oz pack (~106 slides) $25.00
HR8-090 12 x 0.96 mm Thick Siliconized circle cover slides 10 oz case (~1,060 slides) $238.00
HR3-239 18 x 0.22 mm Siliconized circle cover slides 0.5 oz pack (~100 slides) $22.00
HR3-241 18 x 0.22 mm Siliconized circle cover slides 5 oz case (~1,000 slides) $210.00
HR3-515 18 x 0.96 mm Thick Siliconized circle cover slides 1 oz pack (~45 slides) $20.00
HR3-517 18 x 0.96 mm Thick Siliconized circle cover slides 10 oz case (~450 slides) $190.00
HR3-231 22 x 0.22 mm Siliconized circle cover slides 1 oz pack (~120 slides) $25.00
HR3-233 22 x 0.22 mm Siliconized circle cover slides 10 oz case (~1,200 slides) $238.00
HR3-247 22 x 0.96 mm Thick Siliconized circle cover slides 3 oz pack (~75 slides) $27.00
HR3-249 22 x 0.96 mm Thick Siliconized circle cover slides 30 oz case (~750 slides) $256.00
HR3-215 22 x 0.22 mm Siliconized square cover slides 1 oz pack (~100 slides) $22.00
HR3-217 22 x 0.22 mm Siliconized square cover slides 10 oz case (~1,000 slides) $210.00
HR3-223 22 x 0.96 mm Thick Siliconized square cover slides 4 oz pack (~75 slides) $25.00
HR3-225 22 x 0.96 mm Thick Siliconized square cover slides 40 oz case (~750 slides) $238.00
S I L I C O N I Z E D G L A S S C O V E R S L I D E S
Size Thickness Fits
12 VDX48
Size Thickness Fits
12 VDX48
Size Thickness Fits
18 VDXm
Size Thickness Fits
18 VDXm
Size Thickness Fits
22 VDX
Size Thickness Fits
22 VDX
Size Thickness Fits
22 VDX
Thickness Fits
VDX
Size
22
Size Thickness Fits
Flying eagles crystals. A mutant of AppA photoreceptor protein.
Vladimira Dragnea, Department of Biology,
Indiana University, Bloomington, Indiana, USA.
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 92
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C O V E R S L I D E S & R E L A T E D T O O L S
d e s c r i p t i o n
The Cover Slide Gizmo dispenses siliconized or plain slides
for convenient drop building during hanging drop vapor diffu-
sion crystallization. Load the cover slides into the dispenser. A
clear plastic cover or sealed dispenser keeps slides clean. Slide
the loaded dispenser along the platform and six cover slides
are dispensed onto the platform. A black background on the
platform makes for easy drop visualization as one builds the
drop(s). Use finger or forceps to place the completed cover slides onto the crystallization plate.
Each Cover Slide Gizmo includes a single cover so you only need to order HR8-166 if you lose the cover
for the HR8-162 or HR8-164. Manual operation, no power required.
n
Dispense circle or square cover slides for
hanging drop crystallization

n
Dispense siliconized or plain cover slides
n
Platform displays one to six cover slides
at a time for 18 and 22 mm slides
n
Platform displays one to twelve cover slides
at a time for 12 mm slides
n
available for 12, 18 or 22 mm diameter
cover slides for either original (0.22 mm)
or thick (0.96 mm) glass thickness
n
Black platform for slides allows one to see if
drops are clear or precipitated
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR8-167 Cover Slide Gizmo Use for 12 x 0.22 mm circle cover slides $125.00
HR8-168 Cover Slide Gizmo Use for 12 x 0.96 mm circle cover slides $125.00
HR8-169 Cover Slide Gizmo Use for 18 x 0.22 mm circle cover slides $125.00
HR8-170 Cover Slide Gizmo Use for 18 x 0.96 mm circle cover slides $125.00
HR8-162 Cover Slide Gizmo Use for 22 x 0.22 mm circle cover slides $125.00
HR8-163 Cover Slide Gizmo Use for 22 x 0.96 mm circle cover slides $125.00
HR8-164 Cover Slide Gizmo Use for 22 x 0.22 mm square cover slides $125.00
HR8-165 Cover Slide Gizmo Use for 22 x 0.96 mm square cover slides $125.00
HR8-166 Replacement Cover Fits HR8-162 or HR8-164 $15.00
Square Cover Slide Gizmo
Circle Cover Slide Gizmo
G L A S S C O V E R S L I D E G I Z M O D I S P E N S E R
Protein crystal grown using the Hampton Research Crystal Screen.
Enrique Lemus Fuentes, Jefe de carrera de ing. en alimentos,
Universidad Tecnolgica de la Mixteca.
93
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Plain, non-siliconized, hydrophilic glass cover slides. Useful for
hanging drop, sitting drop, and sandwich drop vapor diffusion
crystallization methods. The hydrophilic surface creates a drop
which is more flat than a drop on a siliconized surface. This offers
enhanced imaging for very small drops. Use vacuum grease, DC
Release Compound, or immersion oil to seal cover slide to plate.
n
Hanging, sitting or sandwich drop
crystallization

n
Hydrophilic glass surface
n
12 mm, 18 mm, & 22 mm glass diameter

n
0.22 mm & 0.96 mm glass thickness
n
Circle & square
a p p l i c a t i o n s
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR3-207T 12 mm Plain circle cover slides for Tecan robot 0.5 oz pack (~240 slides) $38.00
HR3-209T 12 mm Plain circle cover slides for Tecan robot 5 oz case (~2,400 slides) $361.00
HR3-235 18 x 0.22 mm Plain circle cover slides 0.5 oz pack (~100 slides) $20.00
HR3-237 18 x 0.22 mm Plain circle cover slides 5 oz case (~1,000 slides) $190.00
HR3-227 22 x 0.22 mm Plain circle cover slides 1 oz pack (~120 slides) $23.00
HR3-229 22 x 0.22 mm Plain circle cover slides 10 oz case (~1,200 slides) $219.00
HR3-243 22 x 0.96 mm Thick Plain circle cover slides 3 oz pack (~75 slides) $25.00
HR3-245 22 x 0.96 mm Thick Plain circle cover slides 30 oz case (~750 slides) $238.00
HR3-211 22 x 0.22 mm Plain square cover slides 1 oz pack (~100 slides) $20.00
HR3-213 22 x 0.22 mm Plain square cover slides 10 oz case (~1,000 slides) $190.00
HR3-219 22 x 0.96 mm Thick Plain square cover slides 4 oz pack (~75 slides) $23.00
HR3-221 22 x 0.96 mm Thick Plain square cover slides 40 oz case (~750 slides) $219.00
P L A I N G L A S S C O V E R S L I D E S
d e s c r i p t i o n
RNase-free, hydrophobic covers are lighter weight than glass.
Plastic cover slides are ready to use without washing or siliconiza-
tion. Hydrophobic working surfaces are protected with clean
release liners to prevent RNase contamination. The plastic cover
slides remain flat and will not curl, even at high temperatures.
Plastic cover slides do not chip, crack, or break and are dust-free.
Square cover slides are supplied protected in a sandwich polyeth-
ylene film. The circle cover slides are packed between fiber-free
paper slips. Use vacuum grease, DC Release Compound, or immer-
sion oil to seal cover slide to plate.
n
Hanging, sitting and sandwich drop
crystallization

n
0.22 mm thickness
n
RNase-free; handle with forceps to prevent
contamination
n
Compatible with most crystallization reagents
n
Siliconization is not required
n
18 mm circle, 22 mm circle,
or 22 mm square
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR8-082 22 mm OptiClear Plastic circle cover slides 100 pack $42.00
HR8-084 22 mm OptiClear Plastic circle cover slides 1,000 pack $373.00
HR8-074 22 mm OptiClear Plastic square cover slides 100 pack $28.00
HR8-076 22 mm OptiClear Plastic square cover slides 1,000 pack $248.00
HR8-078 18 mm OptiClear Plastic circle cover slides 100 pack $42.00
HR8-080 18 mm OptiClear Plastic circle cover slides 1,000 pack $373.00
O P T I C L E A R

P L A S T I C C O V E R S L I D E S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 94
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C O V E R S L I D E S & R E L A T E D T O O L S
d e s c r i p t i o n
d e s c r i p t i o n
d e s c r i p t i o n
PEN-VAC is a great tool for handling small, flat surfaced objects such
as plain and siliconized cover slides, as well as plastic slides. Simply
depress the side button, touch the tip to the object to be lifted and
release the button. PEN-VAC creates a totally self-contained vacuum
that lifts up to 50 grams for up to one minute. Less than an ounce,
the lightweight aluminum body fits in a pocket. No power supply
needed. The kit includes one PEN-VAC with 5 3/4" blue body, (3)
angled and (3) straight probes with (2) each 1/8, 1/4, and 3/8 diameter blue silicone vacuum cups.
It is packaged in a clear plastic storage case.
The Cover Slide Vacuum Gadget allows one to pick up a single
cover slide, invert the slide and gadget, rest the gadget on the
bench so the slide rests as if on a pedestal, pipet the drop onto the
slide, then use the gadget to place and release the slide onto the
crystallization plate. The Gadget is a hand held vacuum bulb with
a single Buna-N vacuum cup. Squeeze the bulb, press the vacuum
cup to the slide, ease the squeeze on the bulb to create a vacuum
and the slide is held in place at the end of the Gadget. When ready
to position and place the slide give the Gadget a gentle squeeze
to release the vacuum. Single bulb with 3/8" (9.53 mm) diameter
cup.
A convenient holder for a crystallization plate, tilted at a 30 angle
to comfortably view samples and pipet without strain. Equipped
with a neoprene pad and rubber feet to prevent slippage. Works
well with smaller, 96 well footprint plates.
n
Holding and manipulating small, flat surfaces
such as glass cover slides

n
Self-contained vacuum
n
No power needed
n
Compact
n
Holding and manipulating small,
flat surfaces such as glass cover slides

n
Self-contained vacuum
n
No power needed
n
Compact
n
Convenient plate holder
a p p l i c a t i o n
a p p l i c a t i o n
a p p l i c a t i o n
f e a t u r e s
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR3-251 PEN-VAC Kit each $95.00
Order Information
Cat. No. Name Description Price
HR8-098 Cover Slide Vacuum Gadget each $27.00
P E N - V A C

C O V E R S L I D E V A C U U M G A D G E T

P L A T E S T A N D
Order Information
Cat. No. Name Description Price
HR4-468 Plate Stand each $41.00
d e s c r i p t i o n
Solid Glass Sitting Drop Rods with a concave depression in the top
can hold up to 50 l and fit into the well of a 24 well VDX Plate
or Linbro

Plate. The rods can stand freely in the well, or a small


amount of vacuum grease can be applied to the bottom of the rod
to secure it to the plate. They also offer enhanced temperature
stability due to the mass of the rod. The rods are 14 mm tall, 10
mm in diameter, and the depth of the concave depression is 2 mm.
The rods are supplied without siliconization.
n
Sitting drop crystallization
n
Perform sitting drop experiments in 24 well
hanging drop plates
n
Made of glass, may be siliconized
n
Thermal mass stabilizes drop temperature
n
Optically clear glass
a p p l i c a t i o n
Order Information
Cat. No. Name Description Price
HR3-146 Glass Sitting Drop Rods 24 pack $100.00
HR3-148 Glass Sitting Drop Rods 144 pack $540.00
f e a t u r e s
m a t e r i a l
ATPase-domain complex of HlyB.
Jelena Zaitseva and Lutz Schmitt, Institute of Biochemistry,
Heinrich Heine University, Duesseldorf, Germany.
95
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Remove dust from cover slides, plates, and other laboratory
supplies.
n
Dust removal
a p p l i c a t i o n
Order Information
Cat. No. Name Description Price
HR4-411 Duster - Canned Air 10 oz can $15.00
D U S T E R - C A N N E D A I R
G L A S S S I T T I N G D R O P R O D S

M I C R O - B R I D G E S

Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 96
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d e s c r i p t i o n
d e s c r i p t i o n
Micro-Bridges are small devices in the shape of a bridge that are
designed to carry out sitting drop vapor diffusion crystallization
when placed in a VDX Plate or Linbro

Plate. A single Micro-


Bridge fits neatly into the reservoir of a standard 24 well VDX Plate
for a sitting drop crystallization experiment. Once placed inside
the wells, Micro-Bridges are stable and there is no need to stick
them to the wells with grease or adhesive. It is therefore possible
to transfer them to other wells during or after a crystallization
experiment. Why sitting drop? Placing the droplet in the indentation greatly reduces the risk of losing
the protein by accident. Crystallization can be carried out in the presence of detergents and organic
solvents which are compatible with polystyrene (such as MPD, iso-propanol, and ethanol). The protein
drop is less affected by condensation problems. Soaking and seeding experiments can be carried out
easily. Crystals can be transported more securely. Larger drop volumes can be used. Micro-Bridges
have a concave indentation in the top surface of the bridge which holds the sample droplet during
a crystallization experiment and prevents the droplet from spreading over a large area. Made from
polystyrene, these parts are highly transparent and suitable for most crystallizations. The surface of the
indentation is highly polished to facilitate the visual inspection of the drops under a microscope. The
maximum drop volume for the Micro-Bridge is 35 l. Reservoirs can be sealed with plain cover slides
and vacuum grease or clear sealing tape.
Same great Micro-Bridges, but manufactured from clarified poly-
propylene. These Micro-Bridges are resistant to most organic
solvents, and are especially useful with crystallization experiments
that involve detergents and other hydrophobic reagents. They
resist drop-spreading typically observed when using reagents such
as detergents, volatile organics such as iso-propanol and ethanol,
and non-volatile organics such as MPD. These Micro-Bridges also
resist acetone, dioxane, acetonitrile, 2,2,2 trifluoroethanol, and
other aggressive organic solvents.
n
Sitting drop crystallization
n
Heavy atom soaks
n
Seeding
n
Perform sitting drop experiments in 24 well
hanging drop plates
n
Removable
n
Optically clear polystyrene
n
Sitting drop crystallization
n
Small molecule crystallization
n
Heavy atom soaks
n
Perform sitting drop experiments in 24 well
hanging drop plates
n
Removable
n
Clarified polypropylene
a p p l i c a t i o n s
a p p l i c a t i o n s
f e a t u r e s
f e a t u r e s
m a t e r i a l
m a t e r i a l
Cover Slide (or Sealing Tape)
Crystallization Droplet
Well of VDX
or Linbro Plate
Reservoir Solution
Micro-Bridge
Order Information
Cat. No. Name Description Price
HR3-310 Micro-Bridges 100 pack $65.00
HR3-312 Micro-Bridges 400 pack $361.00
Order Information
Cat. No. Name Description Price
HR3-340 Micro-Bridges Polypropylene 100 pack $75.00
HR3-342 Micro-Bridges Polypropylene 400 pack $255.00
M I C R O - B R I D G E S

M I C R O - B R I D G E S

P O L Y P R O P Y L E N E
9 W E L L G L A S S P L A T E
S E A L A N T S , S E A L I N G G R E A S E & O I L S
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When setting up a Sandwich Box, one pipets 25 ml of crystallization
reagent or dehydrant into the bottom of the Sandwich Box, then
places a support into the box. The 9 well depression plate is placed
upon the support. Drops of sample and reagent are pipetted into
the depressions and the lid of the Sandwich Box is sealed closed
with vacuum grease. Why Sandwich Boxes? They allow one to use
very, very large drops. They are optically superior to plastic plates
and glass slides, and offer different equilibration kinetics than other
crystallization plates. Each of the Sandwich Box components can be washed and reused so there is
little waste with the Sandwich Box Setup. These plates can be used during screening and optimiza-
tion, but are best suited for final optimization and production of crystals. Since the glass plates can be
removed from the boxes, crystal mounting is convenient. The siliconized 9 well depression plates are
also useful for heavy atom soaks, cryo solution dilution and transfers, and seeding experiments where
serial dilutions are involved. Sandwich Box Setups are available as a complete set or as individual
components so one can customize the system to meet their needs. Square cover slides can be used
to seal individual reservoirs but are not typically used in a crystallization setup. The Sandwich Box
Setup consists of a square plastic box (4 5/16 x 4 5/16 x 1 1/8), a polystyrene plate support, and a
siliconized, glass plate (4 L x 3 3/8 W (100 mm x 85 mm)) with nine concave depressions (7/8 O.D.
x 1/4 D (22 mm x 7 mm)).
References
1. In situ X-ray crystallography. A. McPherson. J. Appl. Cryst. (2000). 33, 397-400.
n
Sitting drop crystallization
n
Seeding
n
Heavy atom soaks
n
Cryo transfers
n
Siliconized, 9 well glass plates
n
Concave wells
n
Polystyrene box and support
n
Siliconized glass and polystyrene
a p p l i c a t i o n s
Order Information
Cat. No. Name Description Price
HR3-136 Sandwich Box Setup contains 6 siliconized, 9 well glass plates, $299.00
6 plastic supports, and 6 sandwich boxes
with covers
HR3-134 Siliconized 9 Well Glass Plate 6 plate pack $273.00
HR3-132 Sandwich Box with cover 40 box case $93.00
f e a t u r e s
m a t e r i a l s
9 W E L L G L A S S P L A T E & S A N D W I C H B O X S E T U P
d e s c r i p t i o n
I M M E R S I O N O I L ( T Y P E A , B A N D N V H )
Useful for sealing cover slides to crystallization plates. Type A oil
has low viscosity (150 centistokes), Type B has medium viscosity
(1250 centistokes), Type 300 has medium/low viscosity (300 cen-
tistokes), and Type NVH has a very high viscosity (21,000 centist-
okes). This works well for incubations above 25C.
n
Useful for sealing cover slides to
crystallization plates
n
Cryoprotection
a p p l i c a t i o n s
Order Information
Cat. No. Name Description Price
HR3-611 Sample Pack Contains the following: Type a - 14.8 ml, Type B - 7.4 ml, $16.00
Type 300 - 7.4 ml, Type NVH - 7.4 ml
HR3-613 Type A 4 oz bottle $16.00
HR3-615 Type B 4 oz bottle $16.00
HR3-617 Type NVH 4 oz bottle $18.00
S E A L A N T S , S E A L I N G G R E A S E & O I L S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
98
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The Dow Corning

7 Release Compound Grease sealant is similar


to vacuum grease but is less viscous. Being less viscous, it is easier
to push through a syringe when applying the sealant manually.
Squeeze the grease into a 10 cc syringe fitted with a 200 l pipet tip
trimmed to desired diameter and you are ready to grease.
D O W C O R N I N G

7 R E L E A S E C O M P O U N D G R E A S E
Order Information
Cat. No. Name Description Price
HR3-508 Dow Corning 7 Release Compound Grease 150 g tube $22.00
d e s c r i p t i o n
R E P L A C E M E N T G R E A S E C A R T R I D G E S
Replacement grease cartridges for the Grease Machine L-100
n
For the Grease Machine L-100
a p p l i c a t i o n
Order Information
Cat. No. Name Description Price
HR3-202 Grease Cartridge 5 pack $56.00
d e s c r i p t i o n
Dow Corning

Vacuum Grease. The standard sealant for hang-


ing, sitting, and sandwich drop vapor diffusion setups. Stiff, non-
melting, non-drying silicone sealant maintains its consistency from
-40 to 260C. Chemical-resistant and non-carbonaceous. Squeeze
the grease into a 10 cc syringe fitted with a 200 l pipet tip trimmed
to desired diameter and you are ready to grease, or try the Grease
Kit.
n
Standard sealant for hanging, sitting,
and sandwich drop vapor diffusion setups
a p p l i c a t i o n
Order Information
Cat. No. Name Description Price
HR3-510 Dow Corning Vacuum Grease 150 g tube $34.00
D O W C O R N I N G

V A C U U M G R E A S E
S E A L I N G F I L M S , T A P E S , M A T S & C O V E R S
99
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C R Y S T A L C L E A R S E A L I N G F I L M
Crystal Clear Sealing Film is an optically transparent sealing film
for SBS format 24, 48, 96 and 384 well plates. The 2 mil (0.05
mm) thick, optically clear, non-fluorescing polyester film with 1
mil (0.025 mm) thick acrylic custom adhesive is designed for the
convenient and secure sealing of crystallization plates. A split,
optically opaque polyester backing with two end tabs assures a
clean, uniform, adhesive layer and allows for easy and accurate
positioning on the plate. Before use, peel off the opaque center
protective polyester backing to reveal the optically clear sealing
film. Film length: 5.625" Film width: 3.125"; End tabs: 0.375"; Perforations: 0.415" from edge of film;
Corner radius: 3/32". DNase-, RNase-, and nucleic-acid-free, non-sterile. Recommended temperatures
from -40 to +120C.
n
Sealing film used to seal sitting drop
crystallization experiments
n
Fits SBS format microplates
n
No special applicator required
n
Optically clear
a p p l i c a t i o n
Order Information
Cat. No. Name Description Price
HR3-609 Crystal Clear Sealing Film 100 pack $108.00
f e a t u r e s
d e s c r i p t i o n
ClearSeal Film fits all SBS format 96 well plates. Minimal evapora-
tion, minimal cross-well contamination, easy to handle, and opti-
cally clear. Single coated, 2 mil (0.05 mm), clear polyolefin film with
pressure-sensitive silicone-based adhesive. Supplied as a 141.3 mm
x 79.4 mm film with polyester release liner (backing). Seals well to
polypropylene, polystyrene, and polycarbonate plates. Compatible
with aqueous and organic solvents. Suitable for use between -70
and 110C. For best seal, the plate must be sealed with the Sealing
Film Applicator. Limited shelf life. Must be used before expiration
date.
The Sealing Film Applicator is a specially designed tool for the
proper application of the ClearSeal Film. The design and rigid plas-
tic allows for the application of even and consistent pressure which
releases the pressure sensitive adhesive on the film to properly seal
the film to the plate.
n
Sealing film used to seal sitting drop
crystallization experiments
n
Optically clear
n
easy to handle
n
Pressure sensitive adhesive
n
For best results use specially designed
applicator
a p p l i c a t i o n
f e a t u r e s
C L E A R S E A L F I L M

A N D A P P L I C A T O R
Order information
Cat. No. Name Description Price
HR4-523 ClearSeal Film 25 pack $56.00
HR4-521 ClearSeal Film 100 pack $198.00
HR4-525 Sealing Film Applicator each $5.00
S E A L I N G F I L M S , T A P E S , M A T S & C O V E R S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 100
d e s c r i p t i o n
The new revolutionary Swissci AG UVP Hanging Drop Crystallization
Plate Seal comes ready-to-use with dust free protective coating and
a specialty polymer base. The seals accommodate up to 3 separate
drops of protein and fit the standard 2 or 3 drop MRC plates. The
product has a 100 micron thin layer of UVP specialty polymer devel-
oped for compact drop creation and ability to shoot x-ray without
any noticeable diffraction.
The seals can be run with drops in the 96, 192 or 288 drop positions. The seal covers sit directly over
the microplate crystallization reagent wells and are closed with a long-term resistant closing adhesive
which is validated not to ingress into sample, nor lead to seal corruption. To remove an individual
crystal, the seal may be peeled back or simply cut out with a scalpel. The material is very thin, although
still remaining as a perfect evaporation barrier and can be sliced with a knife very easily.
The plate seals are produced in a class 10,000 clean room environment and guaranteed to be dust and
scratch free. Optical quality is of the highest level available. The seals are able to be used in the UV
range thus enabling the user to recognize salt from protein crystals under the ultra violet light source
microscope.
n
Hanging drop sealing film for MRC plates
n
Designed for use with the 2 or 3 drop MRC
plates
n
UV compatible
n
X-ray diffraction capability
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR3-607 UVP Hanging Drop MRC Plate Seal 50 pack $470.00
U V P H A N G I N G D R O P M R C P L A T E S E A L
d e s c r i p t i o n
These optically clear tapes are compatible with protein crystal-
lization reagents. The Crystal Clear Sealing Tapes use solvent
based adhesives which are compatible with aqueous crystallization
reagents.
Catalog number HR3-511 is a 1.88 inch (48 mm) wide, 3 mil tape
on a 43.7 yard (40 M) roll with a 1.5 inch core and is supplied with
a green dispenser/cutter.
Catalog number HR4-511 is a 1.88 inch wide, 3 mil tape on a 60 yard
roll with a 3 inch core and no dispenser/cutter.
Two strips of the HR3-511 or HR4-511 will seal a Corning

, Cryschem, CrystalClear Strip, Greiner,


Intelli-Plate, Linbro

, MRC, VDX and VDXm plate.


Catalog number HR4-506 is a 3 inch wide, 3 mil tape on a 54.86 yard roll with a 3 inch core and no
dispenser/cutter.
One strip of the HR4-506 will seal a Corning, Cryschem 24-1 SBS, CrystalClear Strip, Greiner, Intelli-
Plate, MRC and VDXm plate.
See chart for tape and plate compatibility.
n
Sealing tape used to seal sitting drop
crystallization experiments
n
Optically clear
n
Compatible with a wide range of
crystallization reagents
a p p l i c a t i o n
Order Information
Cat. No. Name Description Price
HR3-511 1.88 inch wide Crystal Clear Sealing Tape 1.88 inch roll, x 43.7 yard, $7.00
with cutter
HR4-511 1.88 inch wide Crystal Clear Sealing Tape 1.88 inch x 60 yard roll $9.00
HR4-506 3 inch wide Crystal Clear Sealing Tape 3 inch x 55 yard roll $10.00
f e a t u r e s
Plate 1.88 inch 3 inch
Corning


Cryschem

Cryschem

24-1 SBS
Douglas Instruments
CrystalClear Strips

Greiner
Intelli-Plate

MRC/Swissci

X
X
X
X
X
X
X
C R Y S T A L C L E A R S E A L I N G T A P E
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101
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d e s c r i p t i o n
A 38 m soft non-permeable aluminum foil sealing film with
strong medical-grade adhesive, AlumaSeal II sealing films
eliminate the need for heat-sealing devices or mats during the
resealing of reagents in Deep Well blocks. Each sealing film
measures 82.6 x 142.9 mm and offers sufficient sealing area for
all 96 Deep Well blocks. Length between the perforations with
end tabs removed is 125.4 mm. Compared to other aluminum
foils, AlumaSeal II has less tendency to roll back on itself when
removing the backing paper and conforms well to the plate during application.
AlumaSeal II is a soft, pierceable adhesive film designed for the convenient and rapid sealing of reagent
blocks. A multiple split backing with two end tabs allows for easy, accurate positioning and secure seal-
ing. The use of an adhesive sealing film minimizes evaporation and helps to prevent well-to-well cross
contamination in reagent blocks. AlumaSeal II films are easily pierced by pipettte tips or robotic probes
or piercing tools for direct reagent recovery without significant gumming by adhesive.
Polypropylene mat snap seals MASTERBLOCK 96 Deep Well plate.
n
Sealing film used to reseal HT format
screen kits
n
excellent seal
n
Film conforms to raised chimney wells
n
easily pierceable with single or multichannel
pipetters and robotic probes
n
Less evaporation than clear films
n
Heat & cold resistant, recommended for
temperatures from -80 C to +120 C
n
Certified DNase-, RNase-,
and nucleic-acid-free
n
excellent barrier properties, virtually no
reagent evaporation or drying
n
Snap seal MaSTeRBLOCk 96 Deep Well
plate.
n
Polypropylene
a p p l i c a t i o n
a p p l i c a t i o n
f e a t u r e s
f e a t u r e
Order Information
Cat. No. Name Description Price
HR8-069 AlumaSeal II Sealing Film 100 pack $65.00
HR4-525 Sealing Film Applicator each $5.00
A L U M A S E A L I I S E A L I N G F I L M A N D A P P L I C A T O R
Order information
Cat. No. Name Description Price
HR3-103 Cap Mat for MASTERBLOCK 50 pack $160.00
C A P M A T F O R M A S T E R B L O C K

d e s c r i p t i o n
The Robolid combines the sealing abilities of a silicone rubber mat
and the automation friendliness of a polystyrene lid. Essentially,
it is a silicone mat with tapered well plugs specially bonded to a
polystyrene plate cover. It is compatible with a wide range of liquid
handling automation. The Robolid can be used to cover a 96 Deep
Well block for low evaporation, short-term storage, or to minimize
evaporation or contamination during pipetting. The Robolid is
compatible with the 96 Deep Well blocks used for Hampton Research HT kits such as Crystal Screen
HT, Index HT, and SaltRx HT.
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR3-111 Robolid 25 pack $223.00
R O B O L I D

n
Deep Well block cover for HT screens
n
Lid dimensions are suitable for a wide range
of standard microplates and liquid handling
work stations
n
No well-to-well contamination to ensure
multiple application and removal of lid
n
Silicone sealing plugs are suitable with
organic solvents and low extractables
n
Designed for low evaporation under standard
storage conditions from -20 to 37C
D I A L Y S I S B U T T O N S

, M E M B R A N E S & A P P L I C A T O R S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 102
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In the dialysis method, the sample in question is separated from
the precipitant by a semi-permeable membrane which allows
small reagent molecules to pass but prevents biological macro-
molecules from crossing the membrane.
1-3
Dialysis Buttons are
either machined from transparent perspex or injection molded
from polystyrene, and are the size of a small button. The sample is
placed in this chamber so as to create a slight dome of liquid at the
top of the button. A dialysis membrane is placed over the top of
the button/sample and is held in place with an O-ring. The O-ring
is held in place by a groove in the Dialysis Button. Dialysis Buttons
are supplied with O-rings. The golf tee is supplied with the 5 l to
100 l buttons.
References
1. McPherson, A., Preparation and Analysis of Protein Crystals, Krieger Publishing, 88-91 (1992).
2. Durcruix, A, and Giege, R., Crystallization of Nucleic Acids and Proteins, A Practical Approach,
Oxford University Press, 78-82 (1992).
3. Zeppenzauer, M., et al., Acta Chem Scan (1967) 21, 1009.
n
Crystallization by dialysis, protein
folding, & small volume sample dialysis
n
Low volume dialysis
n
Fits in 24 well plates
n
Use over and over again
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR3-336 Dialysis Buttons Sampler 5 of each size $88.00
HR3-314 5 l Dialysis Button 50 pack $88.00
HR3-316 10 l Dialysis Button 50 pack $88.00
HR3-318 15 l Dialysis Button 50 pack $88.00
HR3-320 20 l Dialysis Button 50 pack $88.00
HR3-322 25 l Dialysis Button 50 pack $88.00
HR3-326 50 l Dialysis Button 50 pack $88.00
HR3-328 100 l Dialysis Button 50 pack $88.00
HR3-330 200 l Dialysis Button 50 pack $88.00
HR3-332 350 l Dialysis Button 50 pack $88.00
How To: Using
Dialysis Buttons
Description
Crystallization by dialysis is an easy variation to the
typical vapor diffusion method used to grow crystals.
In the dialysis method, the sample in question is sep-
arated from the precipitant by a semi-permeable
membrane which allows small molecules such as
ions, additives, buffers, and, salts to pass but pre-
vents biological macromolecules from crossing the
membrane. Equilibration kinetics depend upon the
molecular weight cutoff of the dialysis membrane,
the precipitant, the ratio of the volume, the concen-
tration of the components inside and outside of the
dialysis cell, and the geometry of the cell.
Step 1 - Place sample into dialysis cell
Pipet sample into the dialysis chamber. Amount
of sample can vary depending on the size of the
Dialysis Button.
Step 2 - Placing the membrane
Choose a dialysis membrane that best suits your
molecular weight cutoff of choice. Place the mem-
brane across the top of the Dialysis Button. Using a
golf tee or applicator, press down on the membrane
firmly, making sure no air is trapped between the
membrane and dialysis chamber. Roll the O-ring
over the golf tee and onto the Dialysis Button. Make
sure the O-ring is placed securely in the O-ring sup-
port, holding down the membrane.
Step 3 - Begin dialysis
Place the sealed Dialysis Button into a VDX Plate
or Linbro

Plate. Fill the reservoir with crystalliza-


tion reagent and let the dialysis begin.
Dialysis Chamber
Dialysis Button
O-Ring Support
O-Ring
Golf Tee
Cover Slide (or Sealing Tape)
Well of VDX
Crystallization Plate
Reservoir Solution
Dialysis Button
Vacuum
Grease
D I A L Y S I S B U T T O N S

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The Applicator makes easier work of applying the dialysis mem-
brane and O-ring to Dialysis Buttons. It positions and holds the
dialysis membrane on the buttons and also allows the easy applica-
tion and position of the O-ring to secure the dialysis membrane
onto the Dialysis Button. The Applicator for Dialysis Buttons is
manufactured of glass and is available in two sizes. The Small
Applicator (HR4-348) is designed for use with 5 to 100 l Dialysis
Buttons. The Large Applicator (HR4-350) is designed for use with
200 and 350 l Dialysis Buttons.
n
application of dialysis membrane to Dialysis
Buttons
n
Manufactured of glass
n
Two sizes available
n
Small applicator (5 - 100 l Dialysis
Buttons)

n
Large applicator (200 & 350 l Dialysis
Buttons)
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No: Name: Description Price
HR4-348 Small Applicator for use with 5 to 100 l Dialysis Buttons $47.00
HR4-350 Large Applicator for use with 200 and 350 l Dialysis Buttons $47.00
A P P L I C A T O R F O R D I A L Y S I S B U T T O N S

d e s c r i p t i o n
Spectra/Por

regenerated cellulose dialysis membrane discs are


33 mm, circular, pre-cut membranes available with the following
molecular weight cutoffs:
3,500 MW cutoff
6,000 - 8,000 MW cutoff
12,000 - 14,000 MW cutoff
n
Membranes for dialysis
a p p l i c a t i o n
D I A L Y S I S M E M B R A N E D I S C S

F O R B U T T O N S
Order Information
Cat. No. Name Description Price
HR3-338 Dialysis Membrane Discs cutoff 3,500 - 50 pack $195.00
HR3-344 Dialysis Membrane Discs cutoff 6,000 to 8,000 - 50 pack $195.00
HR3-346 Dialysis Membrane Discs cutoff 12,000 to 14,000 - 50 pack $195.00
HR4-348 Small Applicator for use with 5 to 100 l Dialysis Buttons $47.00
HR4-350 Large Applicator for use with 200 and 350 l Dialysis Buttons $47.00
Protein crystals.
Kasumi Kobayashi, Taiji Nakae and Hiroyuki Akama.
The Kitasato Institute, Kanagawa, Japan.

d e s c r i p t i o n
The Granada Crystallization Box (GCB) consists of
four elements made of polystyrene:
1. A reservoir to introduce the gel
2. A guide to hold capillaries
3. A cover
4. A holder to maintain the boxes
The GCB has been designed to be used in four different ways:
1. To grow crystals inside gels under diffusion-controlled mass transport (figure 1).
2. To grow crystals inside capillaries with un-gelled precipitating agent by the counter-diffusion
technique (figure 2).
3. To grow crystals inside capillaries with gelled precipitating agent by the counter-diffusion
technique (figure 3).
4. To grow crystals inside capillaries by the batch method (figure 4).
Capillaries and gel are not included with the Granada Crystallization Box.
The capillaries described on this page are designed for use with the Granada Crystallization Box. The
capillaries are extremely thin walled. The internal diameter for these capillaries is of 0.1, 0.2 and 0.3
mm. The respective external diameters are 0.17, 0.33 and 0.40 mm. The borosilicate glass capillaries
are available in four different lengths of 30, 40, 50 or 100 mm. Although the capillaries are designed
for use with the Granada Crystallization Box they can also be used for general free-interface diffusion
crystallization.
References
1. Granada Crystallisation Box: a new device for protein crystallisation by counter-diffusion techniques. Garcia-Ruiz JM, Gonzalez-Ramirez LA, Gavira JA,
Otalora F. Acta Crystallogr D Biol Crystallogr. 2002 Oct;58 (Pt 10 Pt 1):1638-42.
2. Counterdiffusion methods for macromolecular crystallization. Garcia-Ruiz JM. Methods Enzymol. 2003;368:130-54.
3. A simplified counter diffusion method combined with a 1D simulation program for optimizing crystallization conditions. H. Tanaka, K. Inaka, S. Sugiyama, S.
Takahashi, S. Sano, M. Sato and S. Yoshitomi. J. Synchrotron Rad. (2004). 11, 45-48.
n
Crystallization using gels in capillaries
n
Crystallization inside glass or quartz
capillaries
n
Can be used with or without gel
n
Counter-diffusion or batch method
n
Optically clear polystyrene
a p p l i c a t i o n
Order Information
Cat. No. Name Description Price
HR3-194 Granada Crystallization Box 20 plates and 1 holder $261.00
HR4-677 Round Capillary 100 mm x 0.1 mm - 25 pack $30.00
HR4-678 Round Capillary 100 mm x 0.2 mm - 25 pack $30.00
HR4-679 Round Capillary 100 mm x 0.3 mm - 25 pack $30.00
HR4-680 Round Capillary 50 mm x 0.1 mm - 25 pack $26.00
HR4-681 Round Capillary 50 mm x 0.2 mm - 25 pack $26.00
HR4-682 Round Capillary 50 mm x 0.3 mm - 25 pack $26.00
HR4-683 Round Capillary 40 mm x 0.1 mm - 25 pack $26.00
HR4-684 Round Capillary 40 mm x 0.2 mm - 25 pack $26.00
HR4-685 Round Capillary 40 mm x 0.3 mm - 25 pack $26.00
HR4-686 Round Capillary 30 mm x 0.1 mm - 25 pack $26.00
HR4-687 Round Capillary 30 mm x 0.2 mm - 25 pack $26.00
HR4-688 Round Capillary 30 mm x 0.3 mm - 25 pack $26.00
HR8-092 LM Agarose 10 g bottle $58.00
figure 1
figure 2
figure 3
figure 4
f e a t u r e s
m a t e r i a l
G R A N A D A C R Y S T A L L I Z A T I O N B O X

& C A P I L L A R I E S
104
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Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
Crystals of the Deinococcus radiodurans 50S ribosome subunit.
From the group of Paola Fucini, Max Planck Institute
for Molecular Genetics, Berlin, Germany.
Protein crystals.
Kasumi Kobayashi, Taiji Nakae and Hiroyuki Akama.
The Kitasato Institute, Kanagawa, Japan.
Deoxyribonuclease crystals grown in dAMP, GDP,
naladixic acid, 15% w/v Polyethylene glycol 3,350.
John Day, University of California Irvine, USA.
t o o l s , s e e d i n g & r e s i n
Crystal image UFO.
Chongping Chen, Dr. Jaffe Lab, Fox Chase Cancer Research Center.
T A B L E O F C O N T E N T S
108 m i c r o - t o o l s

109 m i c r o - t o o l s I I

110 - 113 f o r c e p s
113 c r y s t a l p r o b e

113 c r y s t a l p e n c i l

114 s e e d b e a d

115 s e e d i n g t o o l
115 c h e l a t i n g r e s i n
P A G E S
n
Precision instruments for crystal manipulation
n
Made from chemical resistant,
hardened tool steel
n
Interchangeable tools tips
M I C R O - T O O L S

Crystal shown being cut using the Hampton Research


Micro-Knife.
Courtesy of Lisa Edberg.
University of Alabama, Birmingham
Center for Macromolecular Crystallography
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
a p p l i c a t i o n
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108
s u c c e s s s t o r y
Micro-Tools are the smallest available precision instruments for
laboratory use. These tools are realistically proportioned for mac-
romolecular and small molecule crystallization work. The high
quality, precision Micro-Tools ease microscopic manipulations.
The eight specifically engineered Micro-Tools are useful in a vari-
ety of applications in the crystallization lab. Eight tools, a handle
and wood storage box.
Micro-Tools are designed to be versatile as well as useful for
specific crystallization manipulations. For example, the Micro-Scale is very useful for measuring
crystal dimensions as well as serving as a way to record the dimensions by photography for reference
or publication. The Micro-Chisel and Micro-Spade are helpful for separating and splitting blade and
whisker clusters during seeding or mounting. The Micro-Prober and Micro-Needle come in handy
when probing precipitate or when manipulating small or large crystals for seeding as well as during
mounting. The Micro-Spatula can be used to move crystals as well as split single crystals or clusters.
The Micro-Scraper is helpful when working with very small crystals.
The Micro-Tools Set is indispensable during crystal observation, manipulation, seeding, and mount-
ing. The set includes a single anodized aluminum handle which holds each of the eight unique tips,
all supplied in an attractive alderwood instrument case. The case provides protective storage of the
Micro-Tools as well as a convenient carrying case.
The tips are constructed from hardened tungsten steel and are resistant to most common reagents
used for crystal growth. The threaded tool tips are easily interchanged with the threaded aluminum
handle. The diameter of the tool shaft is 0.250 mm (0.010 inches). The actual dimension at the very
tip of the tool will vary with the particular tool design.
Order Information
Cat. No. Name Description Price
HR4-811 Micro-Tools Set each $298.00
HR4-813 Micro-Scale each $55.00
HR4-815 Micro-Spade each $48.00
HR4-817 Micro-Prober 45 each $48.00
HR4-819 Micro-Scraper each $54.00
HR4-821 Micro-Chisel each $54.00
HR4-823 Micro-Knife 20 each $54.00
HR4-825 Micro-Spatula each $52.00
HR4-827 Micro-Needle each $46.00
HR4-829 Micro-Tool Handle each $33.00
HR4-831 Micro-Tools Carrying Case each $56.00
1
2
3
4
5
1.
2.
3.
7.
4.
6.
8.
5.
Micro-Tool Set:
1. Micro-Scraper
2. Micro-Spatula
3. Micro-Needle
4. Micro-Scale
5. Micro-Spade
6. Micro-Knife 20
7. Micro-Chisel
8. Micro-Prober 45
109
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Precision instruments for crystal manipulation
n
Made from chemical resistant, tungsten steel
and hardened tool steel
n
Interchangeable tools tips
n
8 unique tools designed to extend the
original Micro-Tools set
a p p l i c a t i o n
f e a t u r e s
d e s c r i p t i o n
Micro-Tools II Set is an extension to the smallest available preci-
sion instruments for laboratory use. These tools are realistically
proportioned for macromolecular and small molecule crystal-
lization work. The high quality, precision Micro-Tools II ease
microscopic manipulations. The eight specifically engineered
Micro-Tools II are useful in a variety of applications in the crystal-
lization lab. Eight tools, a handle and wood storage box.
The Micro-Tools II are designed to be versatile as well as useful
for specific crystallization manipulations. For example, the Micro-
Manipulator is handy for manipulating long, thin blades or for sep-
arating long, thin blade clusters. Awkward positions encountered
during crystal manipulation can sometimes be avoided using tools
with an unusual and flexible approach angle such as the Micro-
Prober 90 and the Micro-Hook 90. Seeding experiments can be performed using the Micro-Brush.
The Ultra Micro-Needles are extremely delicate tools crafted from 5 micron radius tungsten that are
very handy for seeding as well as manipulations requiring a soft, fine, and controlled touch.
The Micro-Tools II Set is indispensable during crystal observation, manipulation, seeding, and mount-
ing. The set includes a single anodized aluminum handle which holds each of the eight unique tips,
all supplied in an attractive alderwood instrument case. The case provides protective storage of the
Micro-Tools as well as a convenient carrying case.
The tips are constructed from hardened tungsten steel and are resistant to most common reagents
used for crystal growth. The threaded tool tips are easily interchanged with the threaded aluminum
handle. The diameter of the tool shaft is 0.250 mm (0.010 inches). The actual dimension at the very
tip of the tool will vary with the particular tool design.
Order Information
Cat. No. Name Description Price
HR4-837 Micro-Tools II Set each $298.00
HR4-839 Micro-Manipulator each $49.00
HR4-841 Micro-Knife 45 each $49.00
HR4-843 Micro-Prober 90 each $48.00
HR4-845 Micro-Brush each $44.00
HR4-847 Micro-Hook 90 each $49.00
HR4-849 Ultra Micro-Needle, Straight each $41.00
HR4-851 Ultra Micro-Needle 30 each $49.00
HR4-853 Ultra Micro-Needle 90 each $49.00
HR4-829 Micro-Tool Handle each $33.00
HR4-831 Micro-Tools Carrying Case each $56.00
M I C R O - T O O L S I I

HR4-821
1.
2.
3.
4.
5.
6.
7.
8.
Micro-Tool II Set :
1. Manipulator
2. Micro-Brush
3. Micro-Knife 45
4. Micro-Prober 90
5. Ultra Micro-Needle, straight, tungsten, 5 micron radius
6. Ultra Micro-Needle, bent 30, tungsten, 5 micron radius
7. Micro-Hook 90
8. Ultra Micro-Needle, bent 90, tungsten, 5 micron radius
n
Length - 115 mm
n
Tip - 9.5 mm to point
n
Length - 150 mm
n
Tip - 10.3 mm to point
n
Length - 137 mm
n
Tip - 2.5 mm to point
n
Length - 119 mm
n
Head Length - 11.5 mm
n
Head Width - 6.1 mm
U L T R A - F I N E F O R C E P
S L I D E - T E N S I O N F O R C E P
4 5 A N G L E D F O R C E P
3 0 T I P A N G L E D F O R C E P
F O R C E P S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
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110
f e a t u r e s
f e a t u r e s
f e a t u r e s
f e a t u r e s
High quality, precision straight forceps with an extra fine
point. Smooth, non-serrated tip and handle. Great for
mounting CryoLoops.
Straight forcep with adjustable "locking" tension bar.
Increase the width of the tip opening by setting the bar to
the desired position. Slide bar can be positioned to "lock"
forceps and hold cover slides, CryoLoops, MicroTubes, and
other small parts.
All purpose angled forcep with blunt end. Tip has serration
for firm grip. Good for retrieving. 45 angle makes for easy
manipulation. Especially nice for flipping cover slides. Has
serrated non-slip handle.
Smooth, wide, angled, flat tip that will leave delicate items
undamaged, unlike tweezers with sharp points. Tip is
angled down 30 from the handle. Has serrated, non-slip
handle. Good for handling cover slides and filter paper.
d e s c r i p t i o n
d e s c r i p t i o n
d e s c r i p t i o n
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR4-855 Ultra-Fine Forcep each $10.00
Order Information
Cat. No. Name Description Price
HR4-857 Slide-Tension Forcep each $8.00
Order Information
Cat. No. Name Description Price
HR4-859 45 Angled Forcep each $10.00
Order Information
Cat. No. Name Description Price
HR4-861 30 Tip Angled Forcep each $8.00
111
n
Length - 140 mm
n
Tip Width - 2.5 mm
n
Length - 200 mm
n
Tip Width - 2.5 mm
n
Length - 300 mm
n
Tip Width - 2.5 mm
S M A L L F O R C E P
M E D I U M F O R C E P
E X T R A L A R G E F O R C E P
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f e a t u r e s
f e a t u r e s
All purpose straight forcep with blunt end. Tip has serrated
surface for firm grip. Good for retrieving. Has serrated non-
slip handle.
All purpose straight forcep with blunt end. Tip has serrated
surface for firm grip. Good for retrieving. Has serrated non-
slip handle.
All purpose straight forcep with blunt end. Tip has serrated
surface for firm grip. Good for retrieving. Has serrated non-
slip handle.
d e s c r i p t i o n
d e s c r i p t i o n
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR4-863 Small Forcep each $9.00
Order Information
Cat. No. Name Description Price
HR4-865 Medium Forcep each $13.00
Order Information
Cat. No. Name Description Price
HR4-869 Extra Large Forcep each $10.00
F O R C E P S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
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112
n
Length - 113 mm
n
Tip Length - 31 mm
n
Tip Width - 3.1 mm to point
n
Length - 113 mm
n
Tip Length - 10.9 mm
n
Tip Width - 3.5 mm to point
n
Length - 113 mm
n
Tip Length - Curved to point
n
Length - 156 mm
n
Tip Length - 18 mm
n
Tip Width - 1.7 mm
S T R A I G H T M I C R O F O R C E P
4 5 T I P A N G L E D F O R C E P
C U R V E D T I P M I C R O F O R C E P
A N G L E D T I P L O C K I N G F O R C E P
f e a t u r e s
f e a t u r e s
f e a t u r e s
f e a t u r e s
Ultra-thin, smooth, pointed tip for very precise work. Great
for retrieving or holding small parts. Smooth, non-serrated
handle and tip.
Straight forceps with angled tip. Easy to use forceps when
space is limited. Tip is slanted at a 45 angle. Smooth, non-
serrated handle and tip.
Ultra-thin, smooth, pointed tip for very precise work. Great
for retrieving or holding small parts. Smooth, non-serrated
handle and tip.
All purpose angled tip forcep. Tip has serrated surface for
firm grip. Good for retrieving. When closed and squeezed,
forcep locks to clamp and hold item in place. Lock is
released by moving lock toward tip with thumb. Serrated,
non-slip handle. Angled for easy manipulation.
d e s c r i p t i o n
d e s c r i p t i o n
d e s c r i p t i o n
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR4-871 Straight Microforcep each $13.00
Order Information
Cat. No. Name Description Price
HR4-879 Curved Tip Microforcep each $9.00
Order Information
Cat. No. Name Description Price
HR4-881 Angled Tip Locking Forcep each $13.00
Order Information
Cat. No. Name Description Price
HR4-875 45 Tip Angled Forcep each $8.00
113
a p p l i c a t i o n s
a p p l i c a t i o n s
n
Crystal manipulation
n
Seeding
n
Stainless steel probe with plastic handle
n
Crystal manipulation
n
Seeding
n
Crystal mounting
f e a t u r e s
Disposable Crystal Probe manipulators made from a 0.12
mm x 30 mm stainless steel, pointed-end probe attached
to a 20 mm plastic handle. Useful for breaking apart blade
clusters and general crystal manipulation. Small size and
disposable format makes them convenient for trips to the
synchrotron.
Useful for manipulating crystals for seeding and cryocrystal-
lography. The 0.7 mm tip accepts the Hampton Research
Mounted CryoLoops or MicroTubes with your own
cryoloop which creates an easy to hold, comfortable,
and convenient tool for manipulating crystals. CryoLoops,
MicroTubes, and Mounted CryoLoops sold separately.
d e s c r i p t i o n
d e s c r i p t i o n
C R Y S T A L P E N C I L

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C R Y S T A L P R O B E

n
Length - 156 mm
n
Tip Width- 1.7 mm
L O C K I N G F O R C E P
f e a t u r e s
All purpose straight forcep. Tip has serrated surface for firm
grip. Good for retrieving. When closed and squeezed, for-
cep locks to clamp and hold item in place. Lock is released
by moving lock toward tip with thumb. Serrated, non-slip
handle.
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR4-883 Locking Forcep each $13.00
Order Information
Cat. No. Name Description Price
HR4-217 Crystal Probe 12 pack $15.00
Order Information
Cat. No. Name Description Price
HR4-835 Mechanical Crystal Pencil each $3.00
a p p l i c a t i o n
n
Generate seeds of protein crystals
n
Easily generate consistent seed stocks
n
Use serial dilution to control the number
of seeds introduced into the drop
f e a t u r e s
S E E D B E A D

Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
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The Seed Bead kit is used to create a seed stock for
performing subsequent seeding experiments. The Seed
Bead kit contains 24 Seed Beads manufactured from PTFE,
individually contained in a special 1.5 ml microcentrifuge
tube.
Seeding allows one to grow crystals in the metastable zone.
Crystallization in this zone provides control, reproducibility
and an improved likelihood of a successful crystallization experiment. Also, crystals can grow from seeds
but cannot spontaneously nucleate. By placing a seed or solution of seeds in a drop which is saturated
to the metastable zone, one can use the seeds to grow larger single crystals. By controlling the number
of seeds introduced into the drop, one can control the number of crystals grown. It is not practically
possible to measure and know the number of seeds introduced to a drop, but by performing serial dilu-
tions from a concentrated seed stock, one can control the number of crystals grown in the drop.
Using the Seed Bead kit, one can create crystal seed stock for subsequent seeding experiments. Crystals
are placed in the microcentrifuge tube with the PTFE Seed Bead and either vortexed or sonicated to
generate a seed stock. Then by performing serial dilutions, one can control the number and size of
crystals in the experiment.
The Seed Bead kit is useful for the preparation of seed stocks for automated and semi-automated
microseeding (D'Arcy 2007, Harlos 2008).
Each kit contains 24 special microcentrifuge tubes with Seed Beads. Crystallization accessories are sold
separately.
References
1. Stura, E.A., Wilson, I.A., Methods: A Companion to Methods in Enzymology (1990) 1, 38-49.
2. Stura, E.A., Wilson, I.A., "Seeding Techniques" in Crystallization of Nucleic Acids and Proteins: A Practical Approach. Oxford University Press (1992) 99-126.
3. Luft, J.R., DeTitta, G.T., Poster: Using Ultrasound in the preparation of Micro-Seed Stock for the Crystallization of Macromolecules, 1997 ACA Meeting, St
Louis, MO. Publication in preparation.
4. J.R. Luft and G.T. DeTitta, Methods in Enzymology (1997) 276, 110-131.
5. Structure of an orthorhombic form of xylanase II from Trichoderma reesei and analysis of thermal displacement. Watanabe et al. Acta Cryst. (2006). D62,
784-792.
6. Crystallization and preliminary crystallographic analysis of p40phox, a regulatory subunit of NADPH oxidase. K. Honbou, S. Yuzawa, N. N. Suzuki, Y.
Fujioka, H. Sumimoto and F. Inagaki. Acta Cryst. (2006). F62, 1021-1023 (Used seed bead to optimize)
7. Purification, crystallization and preliminary X-ray diffraction study of human ribosomal protein L10 core domain. Yuji Kobayashi et al. Acta Cryst. (2007). F63,
950952
8. Semi-automated microseeding of nanolitre. Thomas S. Walter, Erika J. Mancini, Jan Kadlec, Stephen C. Graham, Rene Assenberg, Jingshan Ren, Sarah
Sainsbury, Raymond J. Owens, David I. Stuart, Jonathan M. Grimes and Karl Harlos Acta Cryst. (2008). F64, 1418
9. An automated microseed matrix-screening method. Allan DArcy,a* Frederic Villard and May Marsh. Acta Cryst. (2007). D63, 550554
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR2-320 Seed Bead kit 24 tubes with Seed Beads $56.00
Simply pipet your sample into the special 1.5 ml microcentrifuge
tube containing the Seed Bead. Vortex the sample and youve
got seed stock.
115
a p p l i c a t i o n
a p p l i c a t i o n
n
Streak Seeding
n
Natural fiber
n
Built-in handle/cover
n
Remove trace metals from water, reagents,
& samples
n
Scavenge multivalent metal ion contaminants
n
Remove nickel from sample following
purification over a nickel column
f e a t u r e s
f e a t u r e s
During streak seeding, one touches the Seeding Tool to
crystalline material to dislodge, remove and transfer small
crystals (seeds) to a drop that will support the growth of
potentially larger and more perfect crystals. A seed can pro-
vide a template on which additional macromolecules can
assemble and under the proper conditions, grow to form
a large single crystal. Using seeding can avoid problems
associated with growing crystals from spontaneous nucleation. Seeds can grow into larger crystals in
the metastable region of the solubility curve, which is a region of lower, relative supersaturation. One
can also streak seed from phase separation or amorphous material as a diagnostic to confirm whether
the material is crystalline. The Seeding Tool is a 1 cm natural fiber attached to a stainless steel pin and
plastic handle. The Seeding Tool is supplied with a cover to protect the fiber and when the cover is
placed on the back side of the Seeding Tool, it makes an excellent handle.
References
1. Seeds to crystals. Terese Bergfors. Journal of Structrual Biology 142 (2003) 66-76.
2. Applications of the streak seeding technique in protein crystallization. Stura and Wilson. Journal of CrystaL Growth, 110 (1991) 270-282.
Chelating Resin can be used to remove trace metals from water,
reagents, and macromolecular samples
1-3
.
Chelating Resin has a high preference for mercury, copper, nickel,
lead, zinc, cobalt, cadmium, iron, manganese, barium, calcium,
strontium, and magnesium. Its selectivity for divalent ions over
monovalent ions is high (5,000:1) and it has a very high attraction
for transition metals, even in concentrated salt solutions. The resin
will scavenge multivalent metal ion contaminants without altering the concentration of nonmetallic
ions. In most cases the resin does not have any effect on protein concentration or activity.
Chelating Resin is a high purity, 100-200 mesh resin. This resin is a styrene divinylbenzene copolymer
containing paired iminodiacetate ions which act as chelating groups in binding polyvalent metal ions.
The nominal capacity is 2.0 meq/dry gram or 0.4 meq/ml resin bed. The density is 0.65 g/ml. The resin
can be classed with weakly acidic cation exchange resins due to the presence of carboxylic acid groups
but differs from ordinary exchange resins due to its high selectivity for metal ions and high bond
strength. The resin is stable over the entire pH range and functionally active from pH 2 to 14. The resin
is stable for at least two years when stored in the original sealed container at 23C.
References
1. Removal of Nickel from protein solutions following purification over a Nickel column. Hassell, A., Recent Advances in Macromolecular Crystallization,
Bischenberg, FRANCE (1997).
2. Dunn, M.F., Pattison, S.E., Storm, M.C., and Quiel, E., Removal of metals from enzyme solutions., Biochem. (1980) 19, 718.
3. Ray, W.J., Burgner, J.W., and Post, C.B., Purification of NMR reagents., Biochem. (1990) 29, 2770.
d e s c r i p t i o n
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR8-133 Seeding Tool 5 pack $30.00
Order Information
Cat. No. Name Description Price
HR2-312 Chelating Resin 10 g bottle $38.00
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S E E D I N G T O O L
C H E L A T I N G R E S I N

Here is a recipe to try:


Mosaicity is about 0.5
t0 0.6
Reagent:
Crystal Screen Cryo
Reagent 23
Mix equal amounts of
Glucose Isomerase
and reagent. Vapor
diffusion method.

Mount crystal in
CryoLoop.

Mosaicity may be
a bit more in this
reagent and the
unit cell will shrink a
C r y s t a l l i z a t i o n S c r e e n s c r y o c r y s t a l l o g r a p h y
A star of H. influenzae carbonic anhydrase crystals.
Roger S. Rowlett, Department of Chemistry, Colgate University, Hamilton, New York, USA.
T A B L E O F C O N T E N T S
118 - 120 c r y s t a l c a p

h t s y s t e m s
121 - 122 c r y s t a l c a p

m a g n e t i c s y s t e m s
122- 123 c r y s t a l c a p

s y s t e m s
124 - 127 c r y o l o o p s & m i c r o t u b e s
128 - 132 c r y o t o o l s
133 c a n e s , s l e e v e s & c o d e r s
134 - 135 d e w a r s
P A G E S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 118
n
Cryocrystallography
n
Specially designed vial with magnetic ring

n
Cap magnetically attaches to vial
n
Copper jacket to reduce icing on MicroTube
n
Vented vial sold separately
n
No threads
n
Bar coded, color coded, alphanumeric,
magnetic cap
n
Assembled with Mounted CryoLoops,
ready-to-use; vials sold separately
n
Cap features a flat ledge used by grippers
such as SSRL Stanford Automated Mounting
System (SAM)
a p p l i c a t i o n
f e a t u r e s
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C R Y S T A L C A P

H T

S Y S T E M S
CrystalCap Copper Magnetic HT is designed to prevent ice forma-
tion along the copper pin/MicroTube in a cryostream where
the flow is positioned perpendicular, at an angle, or non-collinear
to the MicroTube. The CrystalCap Copper Magnetic HT is a non-
threaded, 1.8 ml (approximate) cryo vial storage container with a
specially engineered cap/pin for cryocrystallography. Each cap is
manufactured from an alloy base which allows the cap to be mag-
netically secured to a magnetic base in the goniometer head. Into
the alloy cap is threaded and bonded a solid, 3 mm diameter copper pin. The end of the copper pin
is machined to an aerodynamic 60 and is assembled with a MicroTube and CryoLoop. A ring magnet
is molded into the top end of the vial so that when the cap is positioned in the vial, the ring magnet
holds the cap on the vial during cryogenic storage. The vial has two holes for venting. The 18 mm
designates the distance between the top of the copper pin and the bottom of the CrystalCap Copper
Magnetic where contact is made with the magnetic base.
For crystal transfer under cryo temperature use the 18 mm CryoTong (catalog number HR4-637 for
the 110 mm overall tool length or catalog number HR5-112 for the 180 mm overall tool length).
The cap features a two dimensional bar coded and alphanumeric 16 x 16 data matrix. Each cap is also
color coded.
HR8-181 CrystalCap Copper Magnetic HT is a two dimensional bar coded and alphanumerically
labeled cap with copper pin; no Mounted CryoLoop and no vial.
Note: Caps with Mounted CryoLoops are sold without vials. Vials sold separately.
Color Coded Cap CryoLoop Size
Red 0.025 - 0.05 mm
Green 0.05 - 0.1 mm
Yellow 0.1 - 0.2 mm
Blue 0.2 - 0.3 mm
Blue/Red 0.3 - 0.4 mm
Green/Red 0.4 - 0.5 mm
Yellow/Red 0.5 - 0.7 mm
Yellow/Green 0.7 - 1.0 mm
The CrystalCap Copper Magnetic HT is not compatible with the EMBL/ESRF SC3 sample changer. For
EMBL/ESRF SC3 and other SPINE sample changers please use the CrystalCap HT.
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR8-173 CrystalCap Copper Magnetic HT 0.025 - 0.05 mm CryoLoop - 30 pack $195.00
HR8-174 CrystalCap Copper Magnetic HT 0.05 - 0.1 mm CryoLoop - 30 pack $195.00
HR8-175 CrystalCap Copper Magnetic HT 0.1 - 0.2 mm CryoLoop - 30 pack $195.00
HR8-176 CrystalCap Copper Magnetic HT 0.2 - 0.3 mm CryoLoop - 30 pack $195.00
HR8-177 CrystalCap Copper Magnetic HT 0.3 - 0.4 mm CryoLoop - 30 pack $195.00
HR8-178 CrystalCap Copper Magnetic HT 0.4 - 0.5 mm CryoLoop - 30 pack $195.00
HR8-179 CrystalCap Copper Magnetic HT 0.5 - 0.7 mm CryoLoop - 30 pack $195.00
HR8-180 CrystalCap Copper Magnetic HT 0.7 - 1.0 mm CryoLoop - 30 pack $195.00
HR4-904 CrystalCap Magnetic Vial Vial ONLY - 30 pack $84.00
HR8-181 CrystalCap Copper Magnetic HT Cap ONLY - 30 pack $130.00
C R Y S T A L C A P

C O P P E R M A G N E T I C H T
2
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2
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m
m
0.65 mm
9.7 mm
12 mm
9.8 mm
1.5 mm
CrystalCap Copper Magnetic HT
119
n
Cryocrystallography
n
Specially designed vial with magnetic ring

n
Cap magnetically attaches to vial
n
Copper jacket to reduce icing on MicroTube
n
Vented vial sold separately
n
No threads
n
Bar coded, color coded, alphanumeric,
magnetic cap
n
Assembled with Mounted CryoLoops,
ready-to-use; vials sold separately
n
Compatible with ALS style sample handlers,
mounters
n
Cap has ledge free, conical shape used by
grippers in ALS - style automounters. For
use at LBNL Berkeley Center for Structural
Biology, with the PXRR automounter at
Brookhaven NSLS, and at CHESS
a p p l i c a t i o n
f e a t u r e s
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CrystalCap Copper Magnetic ALS HT is designed to prevent ice
formation along the copper pin/MicroTube in a cryostream
where the flow is positioned perpendicular, at an angle, or non-
collinear to the MicroTube. The CrystalCap Copper Magnetic
ALS HT is a non-threaded, 1.8 ml (approximate) cryo vial storage
container with a specially engineered ALS compatible cap/pin for
cryocrystallography. Each ALS style cap is manufactured from an
alloy base which allows the cap to be magnetically secured to a
magnetic base in the goniometer head. Into the alloy cap is threaded and bonded a solid, 3 mm
diameter copper pin. The end of the copper pin is machined to an aerodynamic 60 and is assembled
with a MicroTube and CryoLoop or is available without a MicroTube and CryoLoop (HR8-182). A ring
magnet is molded into the top end of the vial so that when the cap is positioned in the vial, the ring
magnet holds the cap on the vial during cryogenic storage. The vial has two holes for venting. The
18 mm designates the distance between the top of the copper pin and the bottom of the CrystalCap
Copper Magnetic where contact is made with the magnetic base.
For crystal transfer under cryo temperature use the 18 mm CryoTong (catalog number HR4-637 for
the 110 mm overall tool length or catalog number HR5-112 for the 180 mm overall tool length).
The cap features a two dimensional bar coded and alphanumeric 16 x 16 data matrix. Each cap is also
color coded.
HR8-182 is a CrystalCap Copper Magnetic ALS HT cap only. No MicroTube, no CryoLoop, no vial.
Note: Caps with Mounted CryoLoops are sold without vials. Vials available separately.
Color Coded Cap CryoLoop Size
Red 0.025 - 0.05 mm
Green 0.05 - 0.1 mm
Yellow 0.1 - 0.2 mm
Blue 0.2 - 0.3 mm
Blue/Red 0.3 - 0.4 mm
Green/Red 0.4 - 0.5 mm
Yellow/Red 0.5 - 0.7 mm
Yellow/Green 0.7 - 1.0 mm
The ALS style automated sample changer accepts the Hampton Research CrystalCap Copper Magnetic
ALS HT.
The CrystalCap Copper Magnetic ALS HT is not compatible with the EMBL/ESRF SC3 sample changer.
For EMBL/ESRF SC3 and other SPINE sample changers please use the CrystalCap HT.
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR8-184 CrystalCap Copper Magnetic ALS HT 0.025 - 0.05 mm CryoLoop - 30 pack $195.00
HR8-186 CrystalCap Copper Magnetic ALS HT 0.05 - 0.1 mm CryoLoop - 30 pack $195.00
HR8-188 CrystalCap Copper Magnetic ALS HT 0.1 - 0.2 mm CryoLoop - 30 pack $195.00
HR8-190 CrystalCap Copper Magnetic ALS HT 0.2 - 0.3 mm CryoLoop - 30 pack $195.00
HR8-192 CrystalCap Copper Magnetic ALS HT 0.3 - 0.4 mm CryoLoop - 30 pack $195.00
HR8-194 CrystalCap Copper Magnetic ALS HT 0.4 - 0.5 mm CryoLoop - 30 pack $195.00
HR8-196 CrystalCap Copper Magnetic ALS HT 0.5 - 0.7 mm CryoLoop - 30 pack $195.00
HR8-198 CrystalCap Copper Magnetic ALS HT 0.7 - 1.0 mm CryoLoop - 30 pack $195.00
HR4-904 CrystalCap Magnetic Vial Vial ONLY - 30 pack $84.00
HR8-182 CrystalCap Copper Magnetic ALS HT Cap ONLY - 30 pack $130.00
C R Y S T A L C A P

C O P P E R M A G N E T I C A L S H T
2
1
.
5

m
m
2
0

m
m
0.65 mm
9.7 mm
12 mm
9.65 mm
1.5 mm
CrystalCap Copper Magnetic ALS HT
C R Y S T A L C A P

H T

S Y S T E M S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 120 Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
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Cryocrystallography
n
Specially designed vial with magnetic ring

n
Cap magnetically attaches to vial
n
Vented vial sold separately
n
No threads
n
Bar coded, color coded, alphanumeric,
magnetic cap
n
Assembled with Mounted CryoLoops,
ready-to-use; vials sold separately
n
Compatible with automated sample handlers
- SPINE sample changer
- ALS style sample mounters
- Brookhaven National Lab PXRR
automounter
- ESRF automated sample changer
a p p l i c a t i o n
f e a t u r e s
The CrystalCap HT is a complete crystal mount for
manual and automated cryocrystallography. The cap sup-
ports the MicroTube and CryoLoop and is made from a
corrosion resistant magnetic alloy. Chamfered edges on
the cap avoid potential blocking during transfers. The cap
design minimizes material to reduce the cooling/melt-
ing/drying temperature cycle when the sample holder
is transferred. The cap features a two dimensional, bar
coded and alphanumeric 16 x 16 data matrix. The alloy
cap, alloy MicroTube, and synthetic CryoLoop feature an overall sample holder length of 22 mm
(measured from the base of the cap to beam position). The magnetic vial, which is sold separately, is
vented, features chamfered edges for enhanced cap positioning and a magnetic alloy bottom for sta-
bility. The CrystalCap HT is compatible with numerous commercial and academic automated sample
handling systems. It is available as individual components (cap only, vial only, or cap and vial) or as
an assembled crystal mount system (cap, MicroTube, and CryoLoop). The CryoLoop is a 20 micron
diameter, synthetic material.
For crystal transfer under cryo temperature use the 18 mm CryoTong (catalog number HR4-637
for the 110 mm overall tool length or catalog number HR5-112 for the 180 mm overall tool length).
HR8-094 is the Crystal Cap HT cap only, no color code, no two dimensional bar code, no white
background on bottom of cap, no alphanumeric side labeling, and is the cap only, without Mounted
CryoLoop.
Note: Caps with Mounted CryoLoops are sold without vials. Vials available separately.
Color Coded Cap CryoLoop Size
Red 0.025 - 0.05 mm
Green 0.05 - 0.1 mm
Yellow 0.1 - 0.2 mm
Blue 0.2 - 0.3 mm
Blue/Red 0.3 - 0.4 mm
Green/Red 0.4 - 0.5 mm
Yellow/Red 0.5 - 0.7 mm
Yellow/Green 0.7 - 1.0 mm
The CrystalCap HT is compatible with the SPINE sample changer, ALS style sample mounters, the
Brookhaven National Lab PXRR automounter, and the ESRF automated sample changer.
C R Y S T A L C A P

H T ( S P I N E )
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR8-118 CrystalCap HT 0.05 mm CryoLoop - 30 pack $195.00
HR8-120 CrystalCap HT 0.05 - 0.1 mm CryoLoop - 30 pack $195.00
HR8-122 CrystalCap HT 0.1 - 0.2 mm CryoLoop - 30 pack $195.00
HR8-124 CrystalCap HT 0.2 - 0.3 mm CryoLoop - 30 pack $195.00
HR8-126 CrystalCap HT 0.3 - 0.4 mm CryoLoop - 30 pack $195.00
HR8-128 CrystalCap HT 0.4 - 0.5 mm CryoLoop - 30 pack $195.00
HR8-130 CrystalCap HT 0.5 - 0.7mm CryoLoop - 30 pack $195.00
HR8-132 CrystalCap HT 0.7 - 1.0 mm CryoLoop - 30 pack $195.00
HR4-637 CryoTong for (same as CryoTong for 18 mm) - 1 each $55.00
CrystalCap HT
HR5-112 CryoTong for (same as Long CryoTong for 18 mm) - 1 each $61.00
CrystalCap HT
HR8-112 CrystalCap HT Cap ONLY - 60 pack $81.00
HR8-114 CrystalCap HT Vial ONLY - 30 pack $84.00
HR8-116 CrystalCap HT Cap/Vial ONLY - 30 pack $155.00
HR8-094 CrystalCap HT Cap ONLY, without bar coding - 30 pack $77.00
0.65 mm
2
1
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5

m
m
12 mm
CrystalCap HT
C R Y S T A L C A P

M A G N E T I C S Y S T E M S
121
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Cryocrystallography
n
Magnetic cap

n
Specially designed vial with magnetic ring
n
Cap magnetically attaches to vial
n
Vented vial
n
No threads
n
Vial without magnetic base
a p p l i c a t i o n
f e a t u r e s
The CrystalCap Magnetic is a non-threaded, 1.8 ml (approximate)
cryo vial storage container with a specially engineered cap for
cryocrystallography. Each cap is manufactured from an alloy base
which allows the cap to be magnetically secured to a magnetic base
in the goniometer head. A ring magnet is molded into the top end
of the vial so that when the cap is positioned in the vial, the ring
magnet holds the cap on the vial during cryogenic storage. The tip
of the metal cap is ready to accept a MicroTube and CryoLoop
or Mounted CryoLoop. CrystalCap Magnetic is available with or without a vial. The vial contains two
small holes for venting.
CrystalCap Magnetic ALS (HR4-779) is compatible with ALS style sample changers.
In the comparison image above, the CrystalCap Magnetic (notice ledge at bottom of taper) appears on
the left while the CrystalCap Magnetic ALS (no ledge at bottom of taper) appears on the right. Both
cap styles can accept and use the same CrystalCap Magnetic Vial.
Brookhaven National Lab PXRR automounter is compatible with the CrystalCap Magnetic ALS.
The automounter at the Berkeley Center for Structural Biology at Lawrence Berkeley National Labs is
compatible with the CrystalCap Magnetic ALS.
ALS style sample mounters accept the CrystalCap Magnetic ALS.
The ESRF automated sample changer accepts the Hampton Research CrystalCap Magnetic with an 18
mm mounted cryoloop.
C R Y S T A L C A P

M A G N E T I C
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR4-731 CrystalCap Magnetic with Vial - 10 pack $60.00
HR4-733 CrystalCap Magnetic with Vial - 60 pack $300.00
HR4-902 CrystalCap Magnetic without Vial - 60 pack $145.00
HR4-779 CrystalCap Magnetic ALS without Vial - 60 pack $150.00
HR4-904 CrystalCap Magnetic Vial Vial ONLY - 30 pack $84.00
Fab crystals growing on a thread.
Allen B. Edmundson,
Oklahoma Medical Research Foundation, USA.
9.7 mm
2
1
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5

m
m
12 mm
0.65 mm
9.8 mm
2
1
.
5

m
m
0.65 mm
9.7 mm
12 mm
9.65 mm
CrystalCap Magnetic
CrystalCap Magnetic ALS
C R Y S T A L C A P

M A G N E T I C S Y S T E M S
C R Y S T A L C A P

S Y S T E M S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 122
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Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
n
Cryocrystallography
n
Magnetic cap

n
Specially designed vial with magnetic ring
n
Cap magnetically attaches to vial
n
Copper jacket to reduce icing on MicroTube
n
Vented vial
n
No threads
n
Vial without magnetic base
a p p l i c a t i o n
f e a t u r e s
n
Cryocrystallography
n
Cap threads to vial

n
Vented cap
n
Magnetic base plate in cap for attachment
to a magnetic base
n
Cap accepts 0.65 mm diameter MicroTube
or Mounted CryoLoop
a p p l i c a t i o n
f e a t u r e s
CrystalCap Copper Magnetic is designed to prevent ice formation
along the copper pin/MicroTube in a cryostream where the flow
is positioned perpendicular, at an angle, or non-collinear to the
MicroTube. The CrystalCap Copper Magnetic is a non-threaded,
1.8 ml (approximate) cryo vial storage container with a specially
engineered cap/pin for cryocrystallography. Each cap is manufac-
tured from an alloy base which allows the cap to be magnetically
secured to a magnet base in the goniometer head. Into the alloy
cap is threaded and bonded a solid, 3 mm diameter copper pin.
The end of the copper pin is machined to an aerodynamic 60 and has a 0.65 mm opening designed
to accept a 10 mm MicroTube and CryoLoop or Mounted CryoLoop snapped at the 10 mm mark
to properly position the CryoLoop in the beam. A ring magnet is molded into the top end of the vial so
that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic
storage. The tip of the metal cap is ready to accept a MicroTube and CryoLoop or Mounted CryoLoop.
The vial has two holes in the vial for venting. The sampler pack contains two of each copper pin length
(10, 12, 14, 16, 18, 21, 24 mm). The length is the distance between the top of the copper pin and the
bottom of the CrystalCap Copper Magnetic where contact is made with the magnetic base.
The ESRF automated sample changer accepts the Hampton Research CrystalCap Copper Magnetic
18 mm.
The CrystalCap is a threaded, 1.8 ml cryo vial storage container with
a specially engineered cap for cryocrystallography. Each cap contains
a magnetic base plate which allows the CrystalCap to be magnetically
secured to a magnetic base in the goniometer head. The opposite
end of the CrystalCap is ready to accept a MicroTube and CryoLoop
or Mounted CryoLoop. The CrystalCap lid is vented to allow cryogen
to pass in and out of the container. The CrystalCap is aerodynamically
efficient to minimize ice buildup.
C R Y S T A L C A P

C O P P E R M A G N E T I C
C R Y S T A L C A P

d e s c r i p t i o n
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR4-737 CrystalCap Copper Magnetic - 10 mm with Vial - 30 pack $190.00
HR4-739 CrystalCap Copper Magnetic - 12 mm with Vial - 30 pack $190.00
HR4-741 CrystalCap Copper Magnetic - 14 mm with Vial - 30 pack $190.00
HR4-743 CrystalCap Copper Magnetic - 16 mm with Vial - 30 pack $190.00
HR4-745 CrystalCap Copper Magnetic - 18 mm with Vial - 30 pack $190.00
HR4-747 CrystalCap Copper Magnetic - 21 mm with Vial - 30 pack $190.00
HR4-749 CrystalCap Copper Magnetic - 24 mm with Vial - 30 pack $190.00
HR4-900 CrystalCap Copper Magnetic - 18 mm without Vial - 30 pack $117.00
HR4-904 CrystalCap Magnetic Vial Vial ONLY - 30 pack $84.00
Order Information
Cat. No. Name Description Price

HR4-911 CrystalCap 10 pack $55.00
HR4-913 CrystalCap 60 pack $285.00
HR4-914 CrystalCap Ex, 0.5 - 0.7 mm 30 pack $225.00
123
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C R Y S T A L C A P

S Y S T E M S
n
Cryocrystallography
n
Cap threads to vial

n
Caps are colored to make identification &
organization easier
n
Vented cap
n
Magnetic base plate in cap for attachment to
a magnetic base
n
Cap accepts 0.65 mm diameter MicroTube
and Mounted CryoLoop
a p p l i c a t i o n
f e a t u r e s
The CrystalCap is now available is six different colors: Red,
Orange, Yellow, Green, Blue, and Pink. The vial container
remains clear. Only the cap lid is colored. Same CrystalCap
features are color coded to make identification, storage, and
recovery faster, easier, and less confusing.
The CrystalCap is a threaded, 1.8 ml cryo vial storage container
with a specially engineered cap for cryocrystallography. Each cap
contains a magnetic base plate which allows the CrystalCap to be
magnetically secured to a magnetic base in the goniometer head. The CrystalCap lid is vented to allow
cryogen to pass in and out of the vial container.
C R Y S T A L C A P

- C O L O R E D
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR8-014 CrystalCap Colored - Sampler 60 pack (10 of each color) $300.00
HR8-002 CrystalCap Colored - Red 60 pack $300.00
HR8-004 CrystalCap Colored - Orange 60 pack $300.00
HR8-006 CrystalCap Colored - Yellow 60 pack $300.00
HR8-008 CrystalCap Colored - Green 60 pack $300.00
HR8-010 CrystalCap Colored - Blue 60 pack $300.00
HR8-012 CrystalCap Colored - Pink 60 pack $300.00
CrystalCap Copper is designed to prevent ice formation when using
the MicroTube in a cryostream where the flow is positioned per-
pendicular, at an angle, or non-collinear to the MicroTube. The
CrystalCap Copper uses a 1.8 ml cryo vial storage container and a
cap which contains a sealed, zinc-plated metal core which allows
the CrystalCap Copper to be secured to a magnetic platform on the
goniometer head. Threaded into the metal base is a solid, 3 mm
diameter copper pin. The end of the copper pin is machined to an
aerodynamic 60 and has a 0.65 mm opening to accept ONLY a 10 mm MicroTube and CryoLoop
or Mounted CryoLoop snapped at the 10 mm mark. The CrystalCap Copper lid is vented to allow
cryogen to pass in and out of the container. The length (10, 12, 14, 16, 18, 21, or 24 mm) is the dis-
tance between the top of the copper pin and the bottom of the CrystalCap Copper where contact is
made with the magnetic base.
Order Information
Cat. No. Name Description Price
HR4-969 CrystalCap Copper - 10 mm 30 pack $180.00
HR4-971 CrystalCap Copper - 12 mm 30 pack $180.00
HR4-973 CrystalCap Copper - 14 mm 30 pack $180.00
HR4-665 CrystalCap Copper - 16 mm 30 pack $180.00
HR4-975 CrystalCap Copper - 18 mm 30 pack $180.00
HR4-977 CrystalCap Copper - 21 mm 30 pack $180.00
HR4-979 CrystalCap Copper - 24 mm 30 pack $180.00
n
Cryocrystallography
n
Cap threads to vial
n
Copper jacket to reduce icing on MicroTube
n
Vented cap
n
Zinc plated steel base plate for attachment
to a magnetic base
a p p l i c a t i o n
f e a t u r e s
d e s c r i p t i o n
C R Y S T A L C A P

C O P P E R
124
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C R Y O L O O P S & M I C R O T U B E S
Mounted CryoLoops with 10 micron diameter nylon. These nylon
loops are pre-staked to hollow, stainless steel MicroTubes that
are used to mount, freeze, and secure the crystal during cryocrys-
tallographic procedures and x-ray data collection. The MicroTube
is 24 mm in length and is specially engineered with EasySnap
notches at the 10, 12, 14, 18, and 21 mm measures. To obtain
the desired length of MicroTube, simply snap the MicroTube at
the desired length and stake it to the CrystalCap Magnetic or
CrystalCap Copper Magnetic. These nylon loops show minimal
diffraction, are thin for fast freezing, strong, and aerodynamic. The 10 micron Mounted CryoLoop
shows less background diffraction than the 20 micron Mounted CryoLoop. Please note that the
10 micron Mounted CryoLoops are very flexible due to their small diameter. Most data collection
geometries prefer or require a 22 mm length between the base of the cap and the beam. For a 22 mm
overall length using the CrystalCap, CrystalCap Magnetic and CrystalCap HT, snap the MicroTube at
the second notch from the bottom. Snap the MicroTube at the fifth notch from the bottom (notch
closest to the CryoLoop) and attach to an 18 mm CrystalCap Copper or CrystalCap Copper Magnetic
to create a 22mm length between the base of the cap and the beam.
M O U N T E D C R Y O L O O P

- 1 0 M I C R O N
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR4-993 Mounted CryoLoop - 10 micron Sampler - 25 pack (5 of each size) $67.00
HR4-995 Mounted CryoLoop - 10 micron 0.05 - 0.1 mm - 25 pack $67.00
HR4-997 Mounted CryoLoop - 10 micron 0.1 - 0.2 mm - 25 pack $67.00
HR4-999 Mounted CryoLoop - 10 micron 0.2 - 0.3 mm - 25 pack $67.00
HR4-615 Mounted CryoLoop - 10 micron 0.3 - 0.4 mm - 25 pack $67.00
HR4-617 Mounted CryoLoop - 10 micron 0.4 - 0.5 mm - 25 pack $67.00
n
Cryocrystallography
n
Synthetic CryoLoop on stainless steel
MicroTube

n
MicroTube specially engineered with
EasySnap notches
n
Complete range of loop diameters from
0.025 - 1.0 mm
a p p l i c a t i o n
f e a t u r e s
Mounted CryoLoops with 20 micron diameter nylon. These nylon
loops are pre-staked to hollow, stainless steel MicroTubes that
are used to mount, freeze, and secure the crystal during cryocrys-
tallographic procedures and x-ray data collection. The MicroTube
is 24 mm in length and is specially engineered with EasySnap
notches at the 10, 12, 14, 18, and 21 mm measures. To obtain
the desired length of MicroTube, simply snap the MicroTube at
the desired length and stake it to the CrystalCap Magnetic or
CrystalCap Copper Magnetic. These nylon loops show minimal
diffraction, are thin for fast freezing, strong, and aerodynamic. Most data collection geometries prefer
or require a 22 mm length between the base of the cap and the beam. For a 22 mm overall length
using the CrystalCap, CrystalCap Magnetic and CrystalCap HT, snap the MicroTube at the second
notch from the bottom. Snap the MicroTube at the fifth notch from the bottom (notch closest to the
CryoLoop) and attach to an 18 mm CrystalCap Copper or CrystalCap Copper Magnetic to create a
22 mm length between the base of the cap and the beam.
M O U N T E D C R Y O L O O P

- 2 0 M I C R O N
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR4-953 Mounted CryoLoop - 20 micron Sampler - 30 pack $78.00
(5 of each diameter size 0.1 - 1.0 mm)
HR4-313 Mounted CryoLoop - 20 micron 0.025 - 0.05 mm - 25 pack $62.00
HR4-625 Mounted CryoLoop - 20 micron 0.05 - 0.1 mm - 25 pack $62.00
HR4-955 Mounted CryoLoop - 20 micron 0.1 - 0.2 mm - 25 pack $62.00
HR4-957 Mounted CryoLoop - 20 micron 0.2 - 0.3 mm - 25 pack $62.00
HR4-959 Mounted CryoLoop - 20 micron 0.3 - 0.4 mm - 25 pack $62.00
HR4-961 Mounted CryoLoop - 20 micron 0.4 - 0.5 mm - 25 pack $62.00
HR4-963 Mounted CryoLoop - 20 micron 0.5 - 0.7 mm - 25 pack $62.00
HR4-965 Mounted CryoLoop - 20 micron 0.7 - 1.0 mm - 25 pack $62.00
n
Cryocrystallography
n
Synthetic CryoLoop on stainless steel
MicroTube

n
MicroTube specially engineered with
EasySnap notches
n
Complete range of loop diameters from
0.05 - 0.5 mm
a p p l i c a t i o n
f e a t u r e s
125
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Similar to the Mounted CryoLoop in design and func-
tion, the Adjustable Mounted CryoLoop allows one to
bend and orient the CryoLoop to multiple, unique posi-
tions previously unattainable with typical cryo setups. The
Adjustable Mounted CryoLoop is a 20 micron diameter
synthetic loop mounted inside a malleable stainless steel
sleeve, which in turn, is mounted inside the EasySnap
MicroTube.
Using a specially designed L-shaped tool with a positioning notch, one can manipulate the angle and
orientation of the CryoLoop by bending and adjusting the angle of the malleable stainless steel insert
which holds the CryoLoop. The Adjustable Mounted CryoLoop may be positioned several times. Note:
Repeated manipulation will fatigue the sleeve which can lead to failure of the sleeve. The Adjustable
Mounted CryoLoop is designed to work with the CrystalCap System components.
The MicroTube is 24 mm in length and is specially engineered with EasySnap notches at the 10, 12,
14, 18, and 21 mm measures. To obtain the desired length of MicroTube, simply snap the MicroTube
at the desired length and stake it to the CrystalCap Magnetic or CrystalCap Copper Magnetic.
These nylon loops show minimal diffraction, are thin for fast freezing, strong, and aerodynamic. Most
data collection geometries prefer or require a 22 mm length between the base of the cap and the
beam. For a 22 mm overall length using the CrystalCap, CrystalCap Magnetic and CrystalCap HT, snap
the MicroTube at the second notch from the bottom. Snap the MicroTube at the fifth notch from the
bottom (notch closest to the CryoLoop) and attach to an 18 mm CrystalCap Copper or CrystalCap
Copper Magnetic to create a 22 mm length between the base of the cap and the beam.
A D J U S T A B L E M O U N T E D C R Y O L O O P

d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR5-900 Adjustable Mounted CryoLoop Sampler - 30 pack $98.00
(5 of each diameter size 0.1 - 1.0 mm)
HR4-336 Adjustable Mounted CryoLoop 0.025 - 0.05 mm - 25 pack $82.00
HR4-338 Adjustable Mounted CryoLoop 0.05 - 0.1 mm - 25 pack $82.00
HR8-102 Adjustable Mounted CryoLoop 0.1 - 0.2 mm - 25 pack $82.00
HR8-072 Adjustable Mounted CryoLoop 0.2 - 0.3 mm - 25 pack $82.00
HR8-104 Adjustable Mounted CryoLoop 0.3 - 0.4 mm - 25 pack $82.00
HR8-106 Adjustable Mounted CryoLoop 0.4 - 0.5 mm - 25 pack $82.00
HR8-108 Adjustable Mounted CryoLoop 0.5 - 0.7 mm - 25 pack $82.00
HR8-110 Adjustable Mounted CryoLoop 0.7 - 1.0 mm - 25 pack $82.00
HR5-902 Tool for Adjustable each $35.00
Mounted CryoLoop
n
Cryocrystallography
n
Adjustable loop orientation

n
CryoLoops are pre-mounted
n
Synthetic CryoLoop on adjustable stainless
steel MicroTube
n
MicroTube specially engineered with
EasySnap notches
n
Available in 20 micron diameter nylon
n
Customize your CryoLoop orientation
a p p l i c a t i o n
f e a t u r e s
Flower shaped crystal of a hypothetical protein from S.Aureas.
Diana Benetteraj, Nickolay Chirgadze Lab, Clinical Genomics Centre,
University Health Network Max Bell Research Centre, Toronto, Ontario, Canada.
C R Y O L O O P S & M I C R O T U B E S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 126
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The MicroTube is the mount and support for the CryoLoop for crys-
tallographers who prefer to mount their own CryoLoop. It fits into the
pinhole opening on the CrystalCap or CrystalCap Copper (use 10 mm
only) and is secured with epoxy or Super Glue. A CryoLoop is then secured
into the opposite end of the MicroTube. The MicroTube is a laser-cut, 0.65
mm diameter stainless steel hollow tube. They are available in a variety
of lengths to accommodate the wide range of geometries presented by
various x-ray data collection systems. The 18 mm length MicroTube is the
standard length used at most data collection facilities. Try a MicroTube
FitKit pack to determine the best length for your application.
Determining MicroTube Length
Follow the instructions to determine which size works best for you. The following considerations have
been taken in determining the length which is best suited for you:
Most XYZ goniometer heads have 5 mm of Z adjustment. The length of exposed loop stem on a
Mounted CryoLoop is approximately 0.5 - 0.7 mm. When mounted properly, the length of exposed
MicroTube set in a CrystalCap Copper is approximately 1.0 mm.
Recommendations:
Use 18 mm MicroTube with CrystalCap
Use 10 mm MicroTube with CrystalCap Copper 18 mm
CryoLoops in 20 micron diameter nylon. They are used to mount,
freeze, and secure the crystal during cryocrystallographic pro-
cedures and x-ray data collection. These nylon CryoLoops show
minimal diffraction, are thin for fast freezing, strong, and aerody-
namic. Supplied as 10 loops per strip, 6 strips per package. Stake
CryoLoops into MicroTubes using epoxy or Super Glue.
M I C R O T U B E

d e s c r i p t i o n
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR4-928 MicroTube FitKit 6 pack (1 of each size) $25.00
HR4-915 MicroTube - 10 mm 60 pack $59.00
HR4-917 MicroTube - 12 mm 60 pack $59.00
HR4-919 MicroTube - 14 mm 60 pack $59.00
HR4-921 MicroTube - 18 mm 60 pack $59.00
HR4-923 MicroTube - 21 mm 60 pack $59.00
HR4-925 MicroTube - 24 mm 60 pack $59.00
HR4-318 Epoxy Dual Syringe 25 ml tube $10.00
HR4-346 Epoxy & Hardener 35 g tube (2 pack) $9.00
HR4-316 Super Glue 2 g tube $5.00
n
CryoLoop support for CrystalCap
n
Laser-cut steel pin

n
Use to mount CryoLoop to CrystalCap
n
Cryocrystallography
n
Synthetic CryoLoop

n
Complete range of loop diameters
from 0.05 - 1.0 mm
n
20 micron diameter nylon
a p p l i c a t i o n
a p p l i c a t i o n
f e a t u r e s
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR4-941 CryoLoop - 20 micron Sampler - 70 pack (10 of each size) $79.00
HR4-623 CryoLoop - 20 micron 0.05 - 0.1 mm - 60 pack $69.00
HR4-929 CryoLoop - 20 micron 0.1 - 0.2 mm - 60 pack $69.00
HR4-931 CryoLoop - 20 micron 0.2 - 0.3 mm - 60 pack $69.00
HR4-933 CryoLoop - 20 micron 0.3 - 0.4 mm - 60 pack $69.00
HR4-935 CryoLoop - 20 micron 0.4 - 0.5 mm - 60 pack $69.00
HR4-937 CryoLoop - 20 micron 0.5 - 0.7 mm - 60 pack $69.00
HR4-939 CryoLoop - 20 micron 0.7 - 1.0 mm - 60 pack $69.00
Crystal shown being collected using a Hampton Research
CryoLoop.
Picture courtesy of Lisa Edberg. Center for Macromolecular
Crystallography University of Alabama, Birmingham.
C R Y O L O O P S

- 2 0 M I C R O N
127
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CryoLoops are available in 10 and 20 micron diameters. They are
used to mount, freeze, and secure the crystal during cryocrystal-
lographic procedures and x-ray data collection. These synthetic
CryoLoops show minimal diffraction, are thin for fast freezing,
strong, and aerodynamic. Supplied as 10 loops per strip, 6 strips
per package. Stake CryoLoops into MicroTubes using Super Glue
(HR4-316). Please note that the 10 micron CryoLoops are very flex-
ible due to their small diameter.
5 minute, fast drying epoxy for sealing capillary tubes or secur-
ing MicroTubes into CrystalCaps or securing CryoLoops into
MicroTubes. Sets in 5 minutes, can be handled in 15 minutes. Full
bond strength in 1 hour. Requires no heat.
Super Glue for securing CryoLoops into MicroTubes.
C R Y O L O O P S

- 1 0 M I C R O N
E P O X Y
S U P E R G L U E
d e s c r i p t i o n
d e s c r i p t i o n
d e s c r i p t i o n
n
Cryocrystallography
n
Synthetic CryoLoop

n
Complete range of loop diameters
from 0.05 - 0.5 mm
n
10 micron diameter nylon
n
Sealing capillary tubes
n
Securing MicroTubes into CrystalCaps

n
Securing CryoLoops into MicroTubes
n
Staking CryoLoops to MicroTubes
a p p l i c a t i o n
a p p l i c a t i o n s
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR4-981 CryoLoop - 10 micron Sampler - 50 pack (10 of each size) $69.00
HR4-983 CryoLoop - 10 micron 0.05 - 0.1 mm - 60 pack $79.00
HR4-985 CryoLoop - 10 micron 0.1 - 0.2 mm - 60 pack $79.00
HR4-987 CryoLoop - 10 micron 0.2 - 0.3 mm - 60 pack $79.00
HR4-989 CryoLoop - 10 micron 0.3 - 0.4 mm - 60 pack $79.00
HR4-991 CryoLoop - 10 micron 0.4 - 0.5 mm - 60 pack $79.00
Crystal shown being collected using a Hampton Research CryoLoop.
Picture courtesy of Lisa Edberg. Center for Macromolecular Crystallography University of Alabama, Birmingham.
Order Information
Cat. No. Name Description Price
HR4-318 Epoxy Dual Syringe 25 ml tube $10.00
HR4-346 Epoxy & Hardener 35 g tube (2 pack) $9.00
Order Information
Cat. No. Name Description Price
HR4-316 Super Glue 2 g tube $5.00
C R Y O T O O L S
128
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The CryoTong is a tool used to manually transfer a crystal mounted
on a CrystalCap from liquid nitrogen to a magnetic base in a
goniometer head positioned in a cryogenic stream, and then back
to liquid nitrogen. The one-piece, compact CryoTong is available
in two lengths. The standard CryoTong is approximately 110 mm
in length from the edge of the tool head to the end of the handle.
The artery clamp style maintains the CryoTong in the closed posi-
tion until the clamp is squeezed, which opens the opposing heads.
The heads are non-magnetic stainless steel. The handle is magnetic stainless steel. The inside of the
head is machined to closely surround the CrystalCap with loop and crystal in place. A small retaining lip
is machined into the lower portion of the head to prevent the CrystalCap from slipping out when the
tool is in the closed position. The CryoTong can maintain the temperature of the crystal at -160C for
up to 30 seconds during room temperature crystal transfers. The CryoTong is available in seven differ-
ent sizes to fit 10 to 24 mm pin heights. Each size is designed to fit any of the the CrystalCap systems.
Choose the proper CryoTong size based upon the pin length required by the configuration of the x-ray
data collection hardware used. The 18 mm CryoTong (HR4-637) is to be used with the CrystalCap HT
systems and any CrystalCap configured to 18 mm.
C R Y O T O N G

- S T A N D A R D
d e s c r i p t i o n
n
Crystal transfer under cryo temperature
n
Artery clamp style closure

n
30 seconds of cryo temperature
n
7 different sizes
n
110 mm length
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR4-631 CryoTong - 10 mm each $55.00
HR4-633 CryoTong - 12 mm each $55.00
HR4-635 CryoTong - 14 mm each $55.00
HR4-667 CryoTong - 16 mm* each $55.00
HR4-637 CryoTong - 18 mm each $55.00
HR4-639 CryoTong - 21 mm each $55.00
HR4-641 CryoTong - 24 mm each $55.00
*The 16 mm CryoTong only works with the CrystalCap Copper 16 mm and the CrystalCap Copper
Magnetic 16 mm.
Valentines Day crystal.
Igor Nederlof, Key Drug Prototyping BV, The Netherlands.
129
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The CryoTong is a tool used to manually transfer a crystal mounted
on a CrystalCap from liquid nitrogen to a magnetic base in a
goniometer head positioned in a cryogenic stream, and then back
to liquid nitrogen. The one-piece, compact CryoTong is available
in two lengths. The long CryoTong is approximately 180 mm in
overall length. The artery clamp style maintains the CryoTong
in the closed position until the clamp is squeezed, which opens
the opposing heads. The heads are non-magnetic stainless steel.
The handle is magnetic stainless steel. The inside of the head is
machined to closely surround the CrystalCap with loop and crystal in place. A small retaining lip is
machined into the lower portion of the head to prevent the CrystalCap from slipping out when the
tool is in the closed position. The CryoTong can maintain the temperature of the crystal at -160C for
up to 30 seconds during room temperature crystal transfers. The CryoTong is available in seven dif-
ferent sizes to fit 10 to 24 mm pin heights. Each size is designed to fit all CrystalCap systems. Choose
the proper CryoTong size based upon the pin length required by the configuration of the x-ray data
collection hardware used. The 18 mm CryoTong (HR5-112) is to be used with the CrystalCap HT and
any other CrystalCap configured for 18 mm.
HR5-114 Long CryoTong 18 mm, 180 features the head in alignment with the handle of the
CryoTong.
HR5-113 Long CryoTong 18 mm, 45 features the head at a 45 angle to the handle of the CryoTong.
C R Y O T O N G

- L O N G
d e s c r i p t i o n
n
Crystal transfer under cryo temperature
n
Artery clamp style closure

n
30 seconds of cryo temperature
n
7 different sizes
n
180 mm length
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR5-104 Long CryoTong - 10 mm each $61.00
HR5-106 Long CryoTong - 12 mm each $61.00
HR5-108 Long CryoTong - 14 mm each $61.00
HR5-110 Long CryoTong - 16 mm* each $61.00
HR5-112 Long CryoTong - 18 mm each $61.00
HR5-113 Long CryoTong - 18 mm, 45 each $61.00
HR5-114 Long CryoTong - 18 mm, 180 each $61.00
HR5-118 Long CryoTong - 21 mm each $61.00
HR5-120 Long CryoTong - 24 mm each $61.00
*The 16 mm Long CryoTong only works with the CrystalCap Copper 16 mm and the CrystalCap Copper
Magnetic 16 mm.
Christmas tree crystals.
Heidi Roth, Department of Structural Biology,
University of Wuerzburg, Germany.
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 130
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C R Y O T O O L S
The Long CrystalWand is a chrome-plated steel wand, 9.6 mm in
diameter with a magnet molded inside one end. The magnet is
designed to fit the CrystalCap and CrystalCap Copper. The Long
Crystal Wand makes it easy to manipulate, seed, mount, store, and
handle crystals with the CrystalCap System. It has a plastic handle
and is available in two styles (no tab and with tab) and two lengths
(standard 130 mm and long 205 mm overall length).
Note: Long CrystalWand is not to be used with the CrystalCap
Magnetic or CrystalCap Copper Magnetic or CrystalCap HT. Use the CrystalWand Magnetic for these
products.
The CrystalWand Magnetic is designed to be used exclusively
with the CrystalCap Magnetic, CrystalCap Copper Magnetic, and
CrystalCap HT systems during transfer of the caps from the vial
to the goniometer head and from the goniometer to the vial.
The 6 1/2 (165 mm) long chrome plated steel wand features a
plastic housing enclosing a spring tensioned plunger that when
depressed, moves a non-magnetic steel platform away from the
magnet housed in the end of the wand. This causes the steel
CrystalCap to detach from the wand and be placed readily into the
vial or the CryoTong. When the platform is retracted, the wand securely holds the steel CrystalCap
Magnetic. The CrystalWand Magnetic 45 (McMiken Tool) offers the same functionality but is bent at
a 45 angle at approximately 2.5 cm from the magnet end.
C R Y S T A L WA N D

- L O N G
C R Y S T A L WA N D

M A G N E T I C
d e s c r i p t i o n
d e s c r i p t i o n
n
CrystalCap and CrystalCap Copper handling
tool
n
CrystalCap Magnetic & CrystalCap Copper
Magnetic handling tool
a p p l i c a t i o n
a p p l i c a t i o n
Order Information
Cat. No. Name Description Price
HR4-600 Long CrystalWand no Tab, 205 mm each $46.00
HR4-602 Long CrystalWand with Tab, 205 mm each $46.00
Order Information
Cat. No. Name Description Price
HR4-729 CrystalWand Magnetic, Straight each $61.00
HR4-315 CrystalWand Magnetic, 45 (McMiken Tool) each $56.00
The CrystalWand is a chrome-plated steel wand, 9.6 mm in
diameter with a magnet molded inside one end. The magnet is
designed to fit the CrystalCap and CrystalCap Copper. The Crystal
Wand makes it easy to manipulate, seed, mount, store, and handle
crystals with the CrystalCap system. The CrystalWand is available
in two styles (no tab and with tab) and two lengths (standard 130
mm and long 205 mm overall length). The long CrystalWand has
a plastic handle.
Note: CrystalWand is not to be used with the CrystalCap Magnetic
or CrystalCap Copper Magnetic or CrystalCap HT. Use the
CrystalWand Magnetic for these products.
C R Y S T A L WA N D

- S T A N D A R D
d e s c r i p t i o n
n
CrystalCap & CrystalCap Copper
handling tool
a p p l i c a t i o n
Order Information
Cat. No. Name Description Price
HR4-951 CrystalWand no Tab, 130 mm each $36.00
HR4-619 CrystalWand with Tab, 130 mm each $36.00
CrystalWand With Tab
CrystalWand No Tab
131
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The Vial Clamp - Straight is a chrome plated, hemostat style tool.
It has a tip shaped to hold the storage vial of all the CrystalCap
systems straight when the clamp is closed. The clamp can be
locked using the hemostat style closure. This clamp makes it easy
to dip the storage vial into liquid nitrogen for crystal storage. The
complete length of the clamp is straight, with an overall length of
195 mm. When the vial is placed in the clamp, the length of it is
positioned perpendicular, 90 to the length of the clamp.
d e s c r i p t i o n
n
Vial support and manipulation
n
Straight end tip
n
Hemostat style closure
a p p l i c a t i o n
Order Information
Cat. No. Name Description Price
HR4-670 Vial Clamp - Straight each $49.00
f e a t u r e s
V I A L C L A M P

- S T R A I G H T
The Vial Clamp - Curved is a chrome plated, hemostat style tool.
It has a tip shaped to hold the storage vial of all the CrystalCap
systems at an angle when the clamp is closed. The clamp can be
locked using the hemostat style closure. The end of the clamp,
where the vial is held, is curved at either a 45/135 or 110/ 70
angle. When the vial is placed in the clamp, the length of it is
positioned to the clamp at either of those same angles. The overall
length of the clamp is 195 mm.
The 7 1/2 (190 mm) long, 15 ml Tube Clamp is useful for hold-
ing and manipulating 15 ml screw top centrifuge tubes during
cryocrystallographic procedures involving liquid propane and
liquid nitrogen. The jaws of the clamp are designed to fit the out-
side diameter (16 mm) of most 15 ml screw top centrifuge tubes.
When the vial is placed in the clamp, the length of it is positioned
to the clamp at either a 45 or 135 angle, depending upon how
one holds the clamp.
d e s c r i p t i o n
d e s c r i p t i o n
n
Vial support and manipulation
n
Curved end tip
n
Hemostat style closure
n
15 ml vial support & manipulation
n
Curved end tip
n
Hemostat style closure
a p p l i c a t i o n
a p p l i c a t i o n
Order Information
Cat. No. Name Description Price
HR4-671 Vial Clamp - Curved 45/135 each $49.00
HR4-672 Vial Clamp - Curved 110/70 each $75.00
Order Information
Cat. No. Name Description Price
HR4-727 Tube Clamp each $61.00
f e a t u r e s
f e a t u r e s
V I A L C L A M P

- C U R V E D
T U B E C L A M P

C R Y O T O O L S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 132 Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
CrystalCap Holders are convenient stands for holding all CrystalCap
systems in liquid nitrogen dewars during crystal cryogenic proce-
dures. Available in two sizes. Both holders feature spring-clips to
secure the CrystalCap Vials in the holder. Both stands have 3"
of adjustable Z (height) which allows one to raise or lower the
CrystalCaps to the appropriate height, depending upon the liquid
nitrogen level in the dewar. Each holder is supplied with a socket
driver for height adjustment.
500 ml version holds five CrystalCaps, two 15 ml vials (for liquid
propane procedures) and fits into the 500 ml dewar. 1,000 ml ver-
sion holds eight CrystalCaps, two 15 ml vials (for liquid propane
procedures) and fits into the 1,000 ml dewar.
The HR4-705 is approximately 98 mm wide (measured at corners of
hexagon)/92 mm wide (measured at flats of hexagon) by approxi-
mately 120 mm tall.
d e s c r i p t i o n
n
CrystalCap support inside stainless steel
dewars
n
Adjustable height
n
Supports up to 8 CrystalCaps
n
15 ml vial position for liquid propane
n
500 and 1,000 ml versions
a p p l i c a t i o n
f e a t u r e s
C R Y S T A L C A P

H O L D E R
Order Information
Cat. No. Name Description Price
HR4-707 CrystalCap Holder - 500 ml each $109.00
HR4-705 CrystalCap Holder - 1,000 ml each $109.00
HR4-706 Replacement Height Adjustment Tool each $15.00
Protein crystals.
Ivana Tomcova, University of South Bohemia & Academy of Science
of the Czech Republic, Nove Hrady, Czech Republic.
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C A N E S , S L E E V E S & C O D E R S
133
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Aluminum CryoCanes firmly hold CrystalCap, CrystalCap Copper,
CrystalCap Magnetic, and CrystalCap Copper Magnetic vials for
storage in dewar-type, liquid nitrogen freezers. The 5 vial CryoCane
offers base tabs for proper CrystalCap seating whereas the 6 vial
CryoCane offers free alignment. Length of 5 vial version: 11 5/16
(28.7 cm). Length of 6 vial version: 11 13/16 (30 cm).
Colored aluminum tabs slip into the ends of CryoCane holders to
identify a set of vials, a researchers work, or sample. Flat writing
surface allows for additional identification. Available in five differ-
ent colors.
d e s c r i p t i o n
d e s c r i p t i o n
n
Organized storage of CrystalCaps
n
2 styles: 5 or 6 vial holding canes
n
Made of aluminum
n
Compatible with the CrystalCap systems
n
Identify & organize CryoCanes
n
Compatible with the CrystalCap systems
n
Made of aluminum
n
5 colors to choose from
a p p l i c a t i o n
a p p l i c a t i o n
Order Information
Cat. No. Name Description Price
HR4-709 CryoCane 5 Vial Holder 12 pack $24.00
HR4-711 CryoCane 6 Vial Holder 12 pack $26.00
f e a t u r e s
f e a t u r e s
C R Y O C A N E
C R Y O C A N E C O L O R C O D E R S
Order Information
Cat. No. Name Description Price
HR4-722 CryoCane Color Coder - Sampler 60 pack (12 of each color) $50.00
HR4-713 CryoCane Color Coder - White 12 pack $10.00
HR4-715 CryoCane Color Coder - Yellow 12 pack $10.00
HR4-717 CryoCane Color Coder - Blue 12 pack $10.00
HR4-719 CryoCane Color Coder - Green 12 pack $10.00
HR4-721 CryoCane Color Coder - Red 12 pack $10.00
Clear polyvinyl sleeve encloses CrystalCap on a CryoCane Holder
for extra security during handling, transport, and storage. Will not
become brittle during freezing or thawing. Unlike cardboard, will
not shred, tear, or fall to pieces after repeated use. 10 13/16 length
(27.5 cm).
d e s c r i p t i o n
n
Use with CryoCanes
n
Will not become brittle
n
Made of polyvinyl
n
Compatible with the CrystalCap systems
a p p l i c a t i o n
f e a t u r e s
C R Y O S L E E V E
Order Information
Cat. No. Name Description Price
HR4-708 CryoSleeve 12 pack $18.00
D E WA R S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 134 Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
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Low form, glass, shallow dewar for use with materials ranging from
dry ice/acetone to liquid nitrogen. This 600 ml full base hemi-
spherical bottom dewar is a low form with full aluminum protective
coating. Low sidewall allows easier access to cryogen.
Dimensions: ID 140 mm, inside depth 65 mm, OD 165 mm,
total height 95 mm. Accepts 500 ml flask. Manufactured by Pope
Scientific.
For optimum results, temper the Dewar per instructions included with each dewar. Refer to The Care
and Use of Pope Dewar Flasks.
d e s c r i p t i o n
n
Cryogenic procedures
n
Low form, shallow 600 ml base
n
Low sidewall for easier access
n
Manufactured for service of liquid nitrogen
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR5-102 Low Form Dewar each $268.00
G L A S S D E WA R
Unbreakable, 304 stainless steel construction dewars for cryocrys-
tallography. Convenient for the flash freezing of crystals, these
dewars store and transport low temperature liquid gases such as
liquid nitrogen and can withstand temperatures from -196C to
204C. The dewars are impervious to vibrations. In comparison
tests, these stainless steel dewars outperform common metal
dewars by 20% and perform comparable to glass modules. Only
the 1,000 ml dewar comes with a stainless steel carrying handle.
Each dewar is supplied with a stainless steel cover. Covers are also
available separately.
500 ml stainless steel dewar flask (2.5ID, 3.4OD, 7.0 depth, 8 height) - right in the picture. 1,000 ml
stainless steel dewar flask (3.9ID, 4.8 OD, 6.2 depth, 6.9 height) - left in the picture.
d e s c r i p t i o n
n
Cryogenic procedures
n
Made of 304 stainless steel
n
Available in 500 ml and 1,000 ml sizes
n
Compatible with CrystalCap Holders
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR4-695 Dewar Flask - 500 ml with cover each $90.00
HR4-697 Dewar Cover - 500 ml each $20.00
HR4-699 Dewar Flask - 1,000 ml with cover each $120.00
HR4-701 Dewar Cover -1,000 ml each $20.00
S T A I N L E S S S T E E L D E WA R
135
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Spearlab Cryogenic Foam Dewar (800 ml) - Standard: The standard
foam dewar shape is circular, with a protruding handle, so that it
resembles a large teardrop. Each dewar is supplied with a matching
foam lid to insulate the contents from ambient air. Dimensions of
the cylindrical cavity in this vessel are 5.8" in diameter by 2.8" deep,
so that it easily holds 800 ml of liquid nitrogen.
Spearlab Cryogenic Foam Dewar (2 liter) - Tall: The tall foam dewar
shape is a tapered octagon on the outside with a cylindrical inte-
rior. The tapered octagon features a wide, stable base. Each dewar
is supplied with a matching foam lid to insulate the contents from
ambient air. Dimensions of the cylindrical cavity in this vessel are
3.5" in diameter by 12.5" deep, so that it holds approximately 1,970
ml of liquid nitrogen. The outside dimensions of the tall dewar are
15.25" tall with a 6.25" wide top and a 7.75" wide base (measured
on the flats of the octagon). The cover is 6.5" in diameter and 0.5"
thick.
The patent pending design of the Spearlab Cryogenic Foam Dewar
makes it easy to handle and safer to use than a traditional low
profile glass dewar. Also, because of its lower thermal mass, a foam
vessel will cause less boil off when it is filled with liquid nitrogen.
Additionally, the dewar will accumulate less frost during regular
use. The end result is that less liquid nitrogen is consumed.
d e s c r i p t i o n
n
Cryocrystallography
n
Each dewar is supplied with a lid
n
Proprietary foam construction
n
Standard (800 ml) and Tall (2 liter)
a p p l i c a t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR4-673 Cryogenic Foam Dewar (800 ml) - Standard each $100.00
HR4-675 Cryogenic Foam Dewar (2 liter) - Tall each $160.00
F O A M D E WA R
Crystal of a protein-DNA complex that grew over
wells containing 5% PEG 8000.
Dr. Christopher Davies, Medical University of South Carolina, USA.
HR4-673
HR4-675
c a p i l l a r y m o u n t s & s u p p l i e s
Crystal flakes - Crystals from a RNA duplex.
Vanessa Delfosse and Dr Claudine Mayer,
Laboratoire de Recherche Molculaire sur les Antibiotiques, Paris, France.
T A B L E O F C O N T E N T S
138 - 140 c a p i l l a r i e s
140 g l a s s f i b e r s
141 w i c k s
142 c a p i l l a r y s e a l a n t s
143 - 144 w a x e s & c l a y
145 m a g n e t i c m o u n t s & s u p p o r t s
P A G E S
138
n
X-ray data collection
n
Liquid-liquid diffusion crystallization
n
Gel acupuncture crystallization
n
3 glass types
n
Thin walled
C A P I L L A R I E S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
a p p l i c a t i o n s
f e a t u r e s
Crystal Clear Glass and Quartz capillaries that
are extremely thin walled (approximately 10
microns wall thickness). The length of the capil-
lary has a well defined diameter, with one end
having a funnel shape and the other end closed.
Capillaries have a wall thickness of 0.01 mm and
an overall length of 80 mm. Capillaries are avail-
able in a wide range of outside diameters from
0.1 to 2.0 mm (5.0 mm for quartz only). The cap-
illaries are designed to mount, hold, and store
small molecule and biological macromolecular
crystals for x-ray data collection. Capillaries can
also be used for crystal density measurements
and crystal growth experiments. The capillaries
can be sealed tightly against moisture and gases
using wax, epoxy, or other sealing materials.
In determining what glass or quartz capillary
is right for you, please refer to the Linear
Absorption Coefficient cm
-1
table. This table
indicates the amount of radiation that is absorbed
by the capillary during x-ray data collection.
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d e s c r i p t i o n
Glass Type
10
50
Quartz
CuK L Radiation
126.0
71.0
75.8
MoK L Radiation
14.7
7.35
8.2
Softening Temp.
708C
815C
1,730C
Linear Absorption Coefficient cm
-1
how t o: mount i ng cryst als usi ng capi llari es
Step 1 - Choosing a crystal
Locate the crystal of
interest to be mounted
in the capillary. Measure
the size of crystal. This
will determine what
diameter capillary to use.
Step 2 - Cutting the capillary
Select the size of capillary. Score the capillary near
the sealed end using the Capillary Cutting Stone.
Snap the capillary at the score mark. A clean cut
every time!
Step 3 - Make a slug
On the funnel end of the
capillary attach a flexible
adaptor to use a syringe
or pipet. Suck up a small
amount of mother liquor.
This will be your mother
liquor slug to prevent the
crystal from drying out during data collection.
Step 4 - Obtaining the crystal
Remove the capillary
from the drop and
place an air gap into
the capillary. This will
separate the slug from
the crystal.
Place the capillary back into the drop and suck up
the crystal. Try not to obtain too much mother
liquor while picking up the crystal.
Step 5 - Removing excess mother liquor
Using either the Paper Wicks or the MicroWick,
remove any excess mother liquor from around the
crystal. There should only be a minimal amount
of mother liquor surrounding the crystal. This will
help reduce background scattering and reduce
crystal slippage.
Step 6 - Sealing one end
Using either Beeswax or Capillary Wax, seal off the
open end of the capillary. Do not overheat the wax
for this will move or even damage the crystal.
Step 7 - Sealing the other end
Trim off the funnel part of the capillary using the
Capillary Cutting Stone as described in Step 2.
Finally, seal off the open end of the capillary as
described in Step 6.
Using Mounting Clay or an Adjustable Crystal
Mount, and a Brass Pin, place the capillary contain-
ing your crystal onto the goniometer head. You are
now ready to collect data.
139
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Order Information
Cat. No. Name Description Price
HR6-152 Special Glass 10 Capillary 0.1 mm - 25 pack $65.00
HR6-154 Special Glass 10 Capillary 0.2 mm - 25 pack $65.00
HR6-156 Special Glass 10 Capillary 0.3 mm - 25 pack $65.00
HR6-158 Special Glass 10 Capillary 0.4 mm - 25 pack $65.00
HR6-160 Special Glass 10 Capillary 0.5 mm - 25 pack $65.00
HR6-162 Special Glass 10 Capillary 0.6 mm - 25 pack $65.00
HR6-164 Special Glass 10 Capillary 0.7 mm - 25 pack $65.00
HR6-166 Special Glass 10 Capillary 0.8 mm - 25 pack $65.00
HR6-168 Special Glass 10 Capillary 0.9 mm - 25 pack $65.00
HR6-170 Special Glass 10 Capillary 1.0 mm - 25 pack $65.00
HR6-172 Special Glass 10 Capillary 1.5 mm - 25 pack $65.00
HR6-174 Special Glass 10 Capillary 2.0 mm - 25 pack $85.00
Order Information
Cat. No. Name Description Price
HR6-104 Glass Number 50 Capillary 0.1 mm - 25 pack $78.00
HR6-106 Glass Number 50 Capillary 0.2 mm - 25 pack $78.00
HR6-108 Glass Number 50 Capillary 0.3 mm - 25 pack $78.00
HR6-110 Glass Number 50 Capillary 0.4 mm - 25 pack $78.00
HR6-112 Glass Number 50 Capillary 0.5 mm - 25 pack $78.00
HR6-114 Glass Number 50 Capillary 0.6 mm - 25 pack $78.00
HR6-116 Glass Number 50 Capillary 0.7 mm - 25 pack $78.00
HR6-118 Glass Number 50 Capillary 0.8 mm - 25 pack $78.00
HR6-120 Glass Number 50 Capillary 0.9 mm - 25 pack $78.00
HR6-122 Glass Number 50 Capillary 1.0 mm - 25 pack $78.00
HR6-124 Glass Number 50 Capillary 1.5 mm - 25 pack $88.00
HR6-126 Glass Number 50 Capillary 2.0 mm - 25 pack $98.00
Order Information
Cat. No. Name Description Price
HR6-128 Quartz Capillary 0.1 mm - 25 pack $118.00
HR6-130 Quartz Capillary 0.2 mm - 25 pack $118.00
HR6-132 Quartz Capillary 0.3 mm - 25 pack $118.00
HR6-134 Quartz Capillary 0.4 mm - 25 pack $118.00
HR6-136 Quartz Capillary 0.5 mm - 25 pack $118.00
HR6-138 Quartz Capillary 0.6 mm - 25 pack $118.00
HR6-140 Quartz Capillary 0.7 mm - 25 pack $118.00
HR6-142 Quartz Capillary 0.8 mm - 25 pack $118.00
HR6-144 Quartz Capillary 0.9 mm - 25 pack $118.00
HR6-146 Quartz Capillary 1.0 mm - 25 pack $118.00
HR6-148 Quartz Capillary 1.5 mm - 25 pack $118.00
HR6-150 Quartz Capillary 2.0 mm - 25 pack $128.00
HR6-151 Quartz Capillary 2.5 mm - 15 pack $128.00
HR6-175 Quartz Capillary 3.0 mm - 15 pack $240.00
HR6-177 Quartz Capillary 4.0 mm - 5 pack $240.00
HR6-179 Quartz Capillary 5.0 mm - 5 pack $285.00
s p e c i a l g l a s s 1 0 ( S O D A L I ME G L A S S )
g l a s s n u m b e r 5 0 ( B O R O S I L I C AT E )
q u a r t z
C A P I L L A R I E S
G L A S S F I B E R S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
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a.) b.) c.)
40 mm length glass fibers for small molecule crystal mounts.
Available in 0.1 - 0.3 mm and 0.3 - 0.5 mm diameter glass. Simple
to use. Glue the glass fiber to a brass specimen pin. Adhesives for
attaching glass fibers to the brass pins can be found in Capillary
Essentials. Add a very small amount of the adhesive of choice to the
tip of the glass fiber. Carefully bring the fiber into solid contact with
the crystal face and leave in this orientation long enough for the
glue to set firmly. Position onto goniometer head and collect data.
These small (25 mm x 25 mm x 0.65 mm) cutting stones are used
to make clean cuts of glass and quartz capillaries. Use the edge
of the stone to etch the capillary then gently snap the capillary in
two. This is useful for cutting the tips off closed capillaries used for
mounting single crystals for x-ray diffraction analysis or for cutting
capillaries to the desired length.
n
Crystal mounts for small molecules
n
Two sizes: 0.1 - 0.3 mm & 0.3 - 0.5 mm
n
Length: 40 mm
n
The easiest way to cut capillaries
n
Score and snap
a p p l i c a t i o n
a p p l i c a t i o n
f e a t u r e s
f e a t u r e s
d e s c r i p t i o n
d e s c r i p t i o n
C A P I L L A R Y C U T T I N G S T O N E

Order Information
Cat. No. Name Description Price
HR8-030 Glass Fiber 0.1 - 0.3 mm diameter - 25 pack $15.00
HR8-032 Glass Fiber 0.3 - 0.5 mm diameter - 25 pack $15.00
Order Information
Cat. No. Name Description Price
HR4-334 Capillary Cutting Stone each $15.00
140
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W I C K S
141
Sterile Paper Wicks are available in a variety of sizes. Smaller
diameter wicks (in 25 mm & 55 mm lengths) are useful for
wicking away mother liquor from within capillary tubes and
CryoLoops while mounting crystals. Larger diameter wicks (in 25
mm & 55 mm lengths) are useful for removing stray/excess solvent
from crystallization setups.
n
Remove excess mother liquor from inside
capillaries and CryoLoops
n
Remove stray drops
a p p l i c a t i o n s
d e s c r i p t i o n
P A P E R W I C K S

Order Information
Cat. No. Name Description Price
HR4-110 Assorted Paper Wicks - 25 mm XXX Fine - X Fine - 200 wicks $15.00
HR4-122 Paper Wicks - 25 mm XXX Fine - 200 wicks $15.00
HR4-112 Paper Wicks - 25 mm XX Fine - 200 wicks $15.00
HR4-114 Paper Wicks - 25 mm X Fine - 200 wicks $15.00
HR4-116 Paper Wicks - 25 mm Fine - 200 wicks $15.00
HR4-211 Paper Wicks - 55 mm X Fine Long - 100 wicks $15.00
HR4-213 Paper Wicks - 55 mm Medium Long - 100 wicks $15.00
MicroWick is useful for removing mother liquor from inside capil-
laries during crystal mounting. MicroWick is a non-metallic syringe
needle used for filling and removing liquid from capillaries and
micropipettes. MicroWick features a 70 mm tip, 0.164 mm OD,
0.1 mm ID (34 gauge) tip which will fit into most capillaries and
micropipettes with an internal diameter of 0.2 mm or larger. The
MicroWick needle is constructed from a combination of plastic
and fused silica - no metal components are used. The MicroWick
tip elasticity is sturdy and flexible though not unbreakable (much more flexible than glass!). Moderate
bending will not block or damage the needle. The MicroWick luer fitting allows easy coupling to
syringes and syringe filters. Each MicroWick contains one needle and one 1 ml syringe. Crystallization
and capillary accessories sold separately.
n
Remove excess mother liquor from inside
capillaries
a p p l i c a t i o n
d e s c r i p t i o n
M I C R O W I C K

Order Information
Cat. No. Name Description Price
HR4-330 MicroWick each $31.00
C A P I L L A R Y E S S E N T I A L S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
C A P I L L A R Y S E A L A N T S
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142
5 minute, fast drying epoxy for sealing capillary tubes or stak-
ing MicroTubes into CrystalCaps. Sets in 5 minutes, can be
handled in 15 minutes. Full bond strength in 1 hour. Requires no
heat!
Old school cement for sealing glass capillaries when mounting
crystals. Requires no heat!
Super glue for staking glass fibers to brass specimen pins, crystal
mounting, and sticking fingers together.
n
Sealing capillaries
n
Staking MicroTubes into CrystalCaps
n
Mount crystals to glass fibers
n
Sealing capillaries
n
Mount crystals to glass fibers
n
Staking glass fibers to brass specimen pins
n
Staking CryoLoops to MicroTubes
a p p l i c a t i o n s
a p p l i c a t i o n s
a p p l i c a t i o n s
d e s c r i p t i o n
d e s c r i p t i o n
d e s c r i p t i o n
E P O X Y
D U C O

C E M E N T
S U P E R G L U E
Order Information
Cat. No. Name Description Price
HR4-320 Duco Cement 29 ml tube $7.00
Order Information
Cat. No. Name Description Price
HR4-316 Super Glue 2 g tube $5.00
Order Information
Cat. No. Name Description Price
HR4-318 Epoxy Dual Syringe 25 ml tube $10.00
HR4-346 Epoxy & Hardener 35 g tube (2 pack) $9.00
n
Sealing capillaries
n
Keep bees busy
a p p l i c a t i o n s
n
Sealing capillaries
a p p l i c a t i o n
n
Sealing capillaries
a p p l i c a t i o n
Order Information
Cat. No. Name Description Price
HR4-312 Beeswax Stick 7 sticks $30.00
Pure, natural, and soft Beeswax - golden in color. Beeswax has a
melting point of 63C/146F. Beeswax comes in seven easy to peel
wrapped sticks making it very convenient to handle. Very useful
for sealing the ends of capillary tubes when mounting crystals.
B E E S WA X

d e s c r i p t i o n
A hard, brittle wax orange in color useful for sealing crystals in
glass capillaries. Non-malleable. Melt with soldering iron or other
heat device and apply to glass or quartz capillaries. When applied
properly, Capillary Wax will provide an airtight seal. Also referred
to as sticky wax.
C A P I L L A R Y WA X

d e s c r i p t i o n
The Wax Pen is a hot pen, powered by two AA batteries
(supplied). Pressing a switch heats the tips of the pen to a tem-
perature that melts Beeswax and Capillary Wax which are used
for sealing capillaries.
Replacement tips are available in a pack of three different shaped
tips (straight loop, straight flat, and curved loop).
WA X P E N

d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR4-328 Capillary Wax 40 g pack (approximate) $25.00
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WA X E S & C L A Y
143
Order Information
Cat. No. Name Description Price
HR4-342 Wax Pen each $50.00
HR4-344 Wax Pen Replacement Tips 3 tip pack $21.00
C A P I L L A R Y E S S E N T I A L S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
C A P I L L A R Y E S S E N T I A L S WA X E S & C L A Y
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Adhesive, malleable, non-hardening clays have many uses in the
crystallization lab. Useful for holding capillaries during crystal
mounting as well as, stabilizing the capillary in the goniometer
head. Useful for propping open the lids of crystallization plates.
Great for color coding experiments, capillaries, and Linbro

crys-
tallization plates. Each 4 oz brick of clay is individually wrapped.
F O U R C O L O R M O U N T I N G C L A Y
n
Stabilizing the capillary on the goniometer
head or brass specimen pin
n
Color code capillary mounts
a p p l i c a t i o n
d e s c r i p t i o n
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR4-326 Four Color Mounting Clay 16 oz pack $12.00
(4 oz each blue/yellow/red/green)
Soft, malleable, cylindrical wax ropes with similar properties as
mounting clay. Uses include propping open the lids of Linbro

crystallization plates, supporting small items, and stabilizing
capillaries.
R E D S T I C K Y WA X
n
Stabilizing the capillary on the goniometer
head or brass specimen pin
n
Prop open lids of Linbro

plates
n
Make bendable buddies
a p p l i c a t i o n s
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR4-310 Red Sticky Wax 44 wax ropes $20.00
C A P I L L A R Y E S S E N T I A L S
Vvn protein with DNA "diamond clock".
Lucy Doudeva, Hanna Yuan's lab, Academia Sinica, Taiwan.
d e s c r i p t i o n
n
Adjustable, magnetic crystal mounting with
capillaries or fibers
n
Adjustable Z
n
Magnetic mount
n
Fits XYZ Goniometer Heads with Z Platform
or magnetic base
n
Fast, easy convenient crystal positioning
and retrieval
a p p l i c a t i o n
n
Capillary and glass fiber mounts
n
Small molecules and macromolecules
n
Available with or without a platform
a p p l i c a t i o n s
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR8-028 Adjustable Crystal Mount 5 pack $58.00
A D J U S T A B L E C R Y S T A L M O U N T

3 mm brass specimen pins with platform for capillary mounting


using Four Color Mounting Clay or Red Sticky Wax. The
platform has a diameter of 9 mm which adds for extra stability in
maintaining a straight capillary for x-ray data collection. Conforms
to IUCr standards.
B R A S S S P E C I M E N P I N

d e s c r i p t i o n
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M A G N E T I C M O U N T S & S U P P O R T S
145
The Adjustable Crystal Mount is a magnetic piece (approximately
12 mm in height, 9.8 mm in diameter) designed to position a
brass specimen pin (below) and either a capillary or glass fiber
onto a goniometer head for x-ray data collection. The Adjustable
Crystal Mount is engineered to fit onto the Z Platform or magnetic
base within a goniometer head. The recessed bottom properly
positions the Crystal Mount over the center of the goniometer
head and prevents the mount from sliding across the goniometer
head during data collection. The bottom of the Crystal Mount is flat and closed to prevent the brass
specimen pin from protruding through the bottom of the Mount. The magnetic design allows for
quick, convenient, and rapid placement and retrieval of the Crystal Mount. Finally, the Crystal Mount
is designed with a single allen screw which allows the Z-translation of the brass specimen pin to
be adjusted (up and down) and then secured in position by tightening the allen screw using the
Goniometer Head Key.
Order Information
Cat. No. Name Description Price
HR4-661 Brass Specimen Pin 25 pack $32.00
HR4-663 Brass Specimen Pin with Platform 25 pack $46.00
f e a t u r e s
g o n i o m e t e r h e a d s & s u p p l i e s
Crystal of nuclear receptor.
Virginie Chavant, Structural Biology and Genomics Platform, CEBGS / IGBMC, Illkirch France.
T A B L E O F C O N T E N T S
148 x y z h e a t e d g o n i o m e t e r h e a d s
149 x y z h e a t e d g o n i o m e t e r h e a d s u p p l i e s
150 x y z s t a n d a r d g o n i o m e t e r h e a d s
151 - 152 x y z g o n i o m e t e r h e a d s u p p l i e s
153 m a g n e t i c b a s e s u p p o r t

P A G E S
n
Heated goniometer head
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Prevent icing on goniometer head & pin
n
Head height: Cryo: 34.5 mm
Capillary: 38.0 mm
n
Adjustments: X & Y: 2.5 mm
Z: 5 mm
X Y Z H E A T E D G O N I O M E T E R H E A D S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 148
34.5 mm height with Z Platform magnetic base for cryocrystal-
lography. 38.0 mm height with Z Capillary for capillary mounts
(measurements from the top of a lowered Z Platform/Z Capillary
to the point where the base mount of the goniometer head
attaches to the goniometer). 2.5 mm X and Y adjustments, 5
mm Z adjustment. Ceramic heating element in base of goniometer
head maintains the head and mounting pin at a temperature that
prevents icing. Temperature controller requires 120/230 volt AC.
Head is equipped with an ACA/IUCr standard thread and support.
Each XYZ Heated Goniometer Head includes a heated goniometer head, Z capillary mount, Z Platform
9,000 gauss magnetic base, adjustment key, and storage case. Temperature controller, connection cable,
and transformer (120v USA or 230v International) are required and sold separately. Crystallization and
goniometer head accessories available separately.
Base thread is M30, 1 mm/thread.
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d e s c r i p t i o n
Cryo:
27.5 mm
XYZ Short Heated
Goniometer Head with
Cryo Mount
Capillary:
31.0 mm
XYZ Short Heated
Goniometer Head with
Capillary Mount
Heated
G
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meter
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Heated
G
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meter
H
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Capillary:
38.0 mm
Goniometer Head with
Capillary Mount
Cryo:
34.5 mm
Goniometer Head with
Cryo Mount
n
Heated goniometer head
n
Prevent icing on goniometer head & pin
n
Head height: Cryo: 27.5 mm
Capillary: 31.0 mm
n
Adjustments: X & Y: 2.5 mm
Z: 5 mm
27.5 mm height with Z Platform magnetic base for cryocrystal-
lography. 31.0 mm height with Z Capillary for capillary mounts
(measurements from the top of a lowered Z Platform/Z Capillary
to the point where the base mount of the goniometer head
attaches to the goniometer). 2.5 mm X and Y adjustments, 5
mm Z adjustment. Ceramic heating element in base of goniometer
head maintains the head and mounting pin at a temperature that
prevents icing. Temperature controller requires 120/230 volt AC.
Head is equipped with an ACA/IUCr standard thread and support.
Each XYZ Short Heated Goniometer Head includes a short heated goniometer head, Z capillary
mount, Z Platform 9,000 gauss magnetic base, adjustment key, and storage case. Temperature con-
troller, connection cable, and transformer (120v USA or 230v International) are required and sold
separately. Crystallization and goniometer head accessories available separately.
Base thread is M30, 1 mm/thread.
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR4-765 XYZ Short Heated Goniometer Head each $685.00
f e a t u r e s
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR4-767 XYZ Heated Goniometer Head each $685.00
X Y Z H E A T E D G O N I O M E T E R H E A D

X Y Z S H O R T H E A T E D G O N I O M E T E R H E A D

149
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X Y Z H E A T E D G O N I O M E T E R H E A D

S U P P L I E S
Temperature Controller for the XYZ Heated and XYZ Short
Heated goniometer heads. Includes connection cable.
Transformer (power supply) for the XYZ Heated and XYZ Short
Heated goniometer heads. Available for both 120v and 230v
applications.
Replacement connection cable for the XYZ Heated and XYZ Short
Heated goniometer heads. Connects goniometer head to tempera-
ture controller.
Replacement fuse for the temperature controller.
n
Temperature controller for all XYZ heated
heads
n
Power supply for all XYZ heated heads
n
Connection cable for all XYZ heated heads
n
Fuse for temperature controllers
d e s c r i p t i o n
d e s c r i p t i o n
d e s c r i p t i o n
d e s c r i p t i o n
f e a t u r e s
f e a t u r e s
f e a t u r e s
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR4-775 Temperature Controller each $98.00
Order Information
Cat. No. Name Description Price
HR4-669 24V Transformer - 120V each $41.00
HR4-755 24V Transformer - 230V each $45.00
Order Information
Cat. No. Name Description Price
HR4-657 Connection Cable each $11.00
Order Information
Cat. No. Name Description Price
HR4-777 Temperature Controller Fuse 3 pack $8.00
T E M P E R A T U R E C O N T R O L L E R F U S E
C O N N E C T I O N C A B L E
T R A N S F O R M E R
T E M P E R A T U R E C O N T R O L L E R
Cryo:
27.5 mm
XYZ Short Goniometer
Head with Cryo Mount
Capillary:
31.0 mm
XYZ Short Goniometer
Head with Capillary Mount
Capillary:
38.0 mm
Goniometer Head with
Capillary Mount
Cryo:
34.5 mm
Goniometer Head with
Cryo Mount
n
Head height: Cryo: 34.5 mm
Capillary: 38.0 mm
n
Adjustments: X & Y: 2.5 mm
Z: 5 mm
X Y Z S T A N D A R D G O N I O M E T E R H E A D S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 150
34.5 mm height with Z Platform magnetic base for cryocrystal-
lography. 38.0 mm height with Z Capillary for capillary mounts
(measurements from the top of a lowered Z Platform/Z Capillary to
the point where the base mount of the goniometer head attaches
to the goniometer). 2.5 mm X and Y adjustments, 5 mm Z
adjustment. Includes XYZ Goniometer Head, Z Capillary Mount, Z
Platform 9,000 gauss magnetic base, adjustment key, and storage
case. Head is equipped with an ACA/IUCr standard thread and
support.
Base thread is M30, 1 mm/thread.
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Head height: Cryo: 27.5 mm
Capillary: 31.0 mm
n
Adjustments: X & Y: 2.5 mm
Z: 5 mm
27.5 mm height with Z Platform magnetic base for cryocrystal-
lography. 31.0 mm height with Z Capillary for capillary mounts
(measurements from the top of a lowered Z Platform/Z Capillary to
the point where the base mount of the goniometer head attaches
to the goniometer) . 2.5 mm X and Y adjustments, 5 mm Z
adjustment. Includes XYZ Short Goniometer Head, Z Capillary
mount, Z Platform 9,000 gauss magnetic base, medium (6,000
gauss) magnetic base, adjustment key, and storage case. Head is
equipped with an ACA/IUCr standard thread and support.
Base thread is M30, 1 mm/thread.
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR4-643 XYZ Short Standard Goniometer Head each $590.00
f e a t u r e s
f e a t u r e s
Order Information
Cat. No. Name Description Price
HR4-647 XYZ Standard Goniometer Head each $590.00
X Y Z S T A N D A R D G O N I O M E T E R H E A D

X Y Z S H O R T S T A N D A R D G O N I O M E T E R H E A D

Black plastic base threads securely into clear plastic cover and is
threaded to hold IUCr standard thread goniometer heads.
Base thread is M30, 1 mm/thread.
Goniometer head key for use with all XYZ goniometer heads.
This key is included with the purchase of any goniometer head.
Replacement set screws are available for the X and Y translation
(2 mm x 4 mm) and for Z translation collar (2 mm x 1 mm).
d e s c r i p t i o n
d e s c r i p t i o n
d e s c r i p t i o n
f e a t u r e s
f e a t u r e s
f e a t u r e s
151
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X Y Z G O N I O M E T E R H E A D S U P P L I E S
n
Plastic storage case for IUCr standard thread
goniometer heads
n
Works with all XYZ heads
n
Replacement set screws for all XYZ heads
Order Information
Cat. No. Name Description Price
HR6-100 X & Y Translation Set Screw 3 pack $9.00
HR6-102 Z Translation Collar Set Screw 3 pack $9.00
S T O R A G E C A S E F O R G O N I O M E T E R H E A D
G O N I O M E T E R H E A D K E Y
S E T S C R E W S
Order Information
Cat. No. Name Description Price
HR4-753 Storage Case for Goniometer Head each $20.00
Order Information
Cat. No. Name Description Price
HR4-659 Goniometer Head Key each $11.00
X Y Z G O N I O M E T E R H E A D S U P P L I E S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 152
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Replacement Z Capillary Support for all XYZ goniometer heads.
The Adjustable Crystal Mount is a magnetic piece (approximately
12 mm in height, 9.8 mm in diameter) designed to position a brass
specimen pin (below) and either a capillary or glass fiber onto a
goniometer head for x-ray data collection. The Adjustable Crystal
Mount is engineered to fit onto the Z Platform or magnetic base
within a goniometer head. The recessed bottom properly positions
the crystal mount over the center of the goniometer head and pre-
vents the mount from sliding across the goniometer head during
data collection. The bottom of the crystal mount is flat and closed to prevent the brass specimen pin
from protruding through the bottom of the mount. The magnetic design allows for quick, convenient,
and rapid placement and retrieval of the crystal mount. Finally, the crystal mount is designed with a
single allen screw which allows the Z-translation of the brass specimen pin to be adjusted (up and
down) and then secured in position by tightening the allen screw using the Goniometer Head Key.
3 mm brass specimen pins with platform for capillary mount-
ing using Four Color Mounting Clay or Red Sticky Wax.
The platform has a diameter of 9 mm which adds extra stability in
maintaining a straight capillary for x-ray data collection. Conforms
to IUCr standards.
d e s c r i p t i o n
d e s c r i p t i o n
d e s c r i p t i o n
f e a t u r e s
n
Capillary pin support for all XYZ heads
n
Supports brass specimen pins
Order Information
Cat. No. Name Description Price
HR8-028 Adjustable Crystal Mount 5 pack $58.00
a p p l i c a t i o n
a p p l i c a t i o n s
n
Adjustable, magnetic crystal mounting with
capillaries or fibers
n
Adjustable Z
n
Magnetic mount
n
Fits XYZ Goniometer Heads with Z Platform
or magnetic base
n
Fast, easy, and convenient crystal positioning
and retrieval
n
Capillary and glass fiber mounts
n
Small molecules and macromolecules
n
Available with or without platform
f e a t u r e s
Z C A P I L L A R Y

S U P P O R T
A D J U S T A B L E C R Y S T A L M O U N T
B R A S S S P E C I M E N P I N

Order Information
Cat. No. Name Description Price
HR4-661 Brass Specimen Pin 25 pack $32.00
HR4-663 Brass Specimen Pin with Platform 25 pack $46.00
Order Information
Cat. No. Name Description Price
HR4-769 Z Capillary For all XYZ short heads - each $37.00
HR4-771 Z Capillary For all XYZ heads - each $37.00
The CrystalCap Magnetic Base (9.3 mm diameter support) is a
machined brass piece fitted with a magnet. The base fits into
the goniometer head, providing a secure base for the CrystalCap
Magnetic System. Fits most goniometer heads. The CrystalCap
Magnetic Base is available in three strengths: strong, medium, and
light. The stem of the magnet is 3 mm in diameter. The length of
the stem is 5 mm. The thickness of the platform is 3.6 mm.
Z Platform with 6,000 gauss magnet for all XYZ goniometer
heads. Less pull and reduced "snapping" than Z Platform with
9,000 gauss magnet.
Z Platform with 9,000 gauss magnet for all XYZ goniometer
heads. Same Z Platform that is included with the purchase of any
goniometer head.
d e s c r i p t i o n
d e s c r i p t i o n
d e s c r i p t i o n
f e a t u r e s
f e a t u r e s
f e a t u r e s
153
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M A G N E T I C B A S E

S U P P O R T
n
Available in 3 strengths
n
Magnetic support for CrystalCap
n
Light strength (6,000 gauss) magnetic
support for all XYZ heads
n
Magnetic support for CrystalCap
n
Strong strength (9,000 gauss) magnetic
support for all XYZ heads
n
Magnetic support for CrystalCap
Order Information
Cat. No. Name Description Price
HR4-943 Magnetic Base - Strong 9,000 gauss - each $35.00
HR4-627 Magnetic Base - Medium 6,000 gauss - each $35.00
HR4-629 Magnetic Base - Light 4,000 gauss - each $35.00
M A G N E T I C B A S E
M A G N E T I C B A S E S U P P O R T Z P L A T F O R M

- L I G H T
M A G N E T I C B A S E S U P P O R T Z P L A T F O R M

- S T R O N G
Order Information
Cat. No. Name Description Price
HR4-761 Z Platform - Strong For all XYZ short heads - each $35.00
HR4-763 Z Platform - Strong For all XYZ heads - each $35.00
Order Information
Cat. No. Name Description Price
HR4-651 Z Platform - Light For all XYZ short heads - each $37.00
HR4-653 Z Platform - Light For all XYZ heads - each $37.00
x e n o n d e r i v a t i z a t i o n
Protein Crystals.
Anja Lehwe-Litzmann, Institut for Biochemie & Biotechnologie, Martin-Luther-Universitt Halle-Wittenberg.
T A B L E O F C O N T E N T S
156 x e n o n c h a m b e r

157 x e n o n r e c o v e r y s y s t e m

P A G E S
n
Produce xenon derivatives of biological
macromolecular crystals
n
Operation pressure range of 0 to 600 psi
n
Small pressure chamber for minimum xenon
gas consumption
n
Pressure regulated safety valve
n
Safety lock prevents opening of pressurized
chamber
n
Safety shield between pressure chamber
and user
n
Quick release connectors between xenon
gas supply and pressure chamber
n
High quality xenon pressure regulator with
needle valve for fine control
n
Unique track design allows rapid crystal
transfer from xenon to dewar for freezing
n
Mini-Vial/Wick system prevents crystal
dehydration
n
CrystalCap compatible
Order Information
Cat. No. Name Description Price
HR4-791 Xenon Chamber each $2,400.00
HR4-793 Xenon Chamber Pressure Regulator each $560.00
HR4-781 Xenon Chamber Quick Female Connector each $50.00
HR4-795 Xenon Chamber Mini-Vial with Wick 5 pack $75.00
HR4-799 Xenon Chamber Vial Stand each $55.00
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 156
a p p l i c a t i o n
f e a t u r e s
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Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
The Xenon Chamber is a pressure chamber
designed to produce xenon derivatives of biologi-
cal macromolecular crystals.
Aside from the preparation of a pure sample and
subsequent crystallization, an often rate-limiting
step in the determination of biological macromo-
lecular structures by x-ray diffraction analysis is the
formation of isomorphous heavy atom derivatives.
Typically, isomorphous derivatives are formed by
diffusing heavy atoms such as lead or mercury
based compounds into the crystal with the antici-
pation that the heavy atom molecules will bind in
an ordered fashion to each biological macromol-
ecule. In order to obtain two or more successful
isomorphous derivatives, a researcher may need
to evaluate close to 100 different heavy atom
compounds by manually transferring a number of
crystals to different mother liquors each contain-
ing a different heavy atom compound.
Enter Xenon
Xenon is a noble gas which binds to specific sites
in a biological macromolecule. There are numer-
ous examples demonstrating that xenon-macro-
molecule complexes can serve as heavy atom
derivatives.
1-8
The xenon-macromolecule complex
is obtained by placing the macromolecular crystal
under a pressurized xenon atmosphere. A clear
advantage in using xenon is that one can simply
screen xenon by pressurizing a loop-mounted
crystal rather than having to set up plates and hav-
ing to transfer crystals from numerous drops.
Enter the Xenon Chamber
The Xenon Chamber is a simple, yet effective
device designed to pressurize loop-mounted bio-
logical macromolecular crystals in the presence of
xenon gas at room temperature. Crystals mounted
in loops such as the CrystalCap system are placed
into the Xenon Chamber. Once sealed, the cham-
ber is pressurized with xenon gas so that the
crystal and macromolecules are equilibrated in
a vapor saturated xenon atmosphere. Following
depressurization of the chamber, the loop-mount-
ed crystal is simply lifted and slid along the Xenon
Chamber track and quickly lowered into a dewar
for freezing in liquid nitrogen or propane. The
transfer from pressurized xenon to the freeze is
accomplished in seconds.
Specifications
The Xenon Chamber measures 14 1/4" tall, 13 1/2"
wide, and 10 1/4" deep. The pressure chamber
measures 9.5 mm (diameter) by 29 mm (depth).
The Xenon Chamber has an operation pressure
range of 0 to 600 psi (0 to 4.1 Mpa, 0 to 41 Bar).
The pressure regulator for the Xenon Chamber
is designed to be used with inert gases such
as xenon. The CGA number for the pressure
regulator is 580. The pressure regulator has a hose
length of 21" with a quick connector on the end to
attach it to the Xenon Chamber.
Each Xenon Chamber comes complete with a
CrystalCap Dewar Stand that fits the 1,000 ml
Dewar Flask (HR4-699) and a pack of five Mini-
Vials with Wicks. The Xenon Chamber Pressure
Regulator is sold separately. Additional Mini-Vials
with Wick and Vial Stands may also be obtained
separately.
References
1. PNAS, Oct. 12 1999, Vol.96, No. 21, 11717-11722.
2. Soltis, S.M., Stowell, M.H.B., Wiener, M.C., Phillips, G.N. Jr., & Rees, D.C.,
J. Appl. Cryst. (1997) 30, 190-194.
3. Stowell, M.H.B., Soltis, S.M., Kisker, C., Peters, J.W., Schindelin, H., Rees,
D.C., Cascio, D., Beamer, L., Hart, P.J., Wiener, M.C., & Whitby, F.G., J.
Appl. Cryst. (1996) 29, 608-613.
4. Schiltz, M., Fourme, R., Broutin, I., & Prange, T., Structure (1995) 3,
309-316.
5. Schitz, M., Prange, T., & Fourme, R., J. Appl. Cryst. (1994) 27, 950-960.
6. Vitali, J., Robbins, A.H., Almo, S.C., & Tilton, R.F., J. Appl. Cryst. (1991)
24, 931-935.
7. Schiltz, M., Shepard, W., Fourme, R., Prange, T., De La Fortelle, R., &
Bricogne, G., Acta Cryst. (1997) D53, 78-92.
8. Otwinowski, Z. & Minor, W., Methods Enzymol. Vol. 276, edited by C.W.
Carter, Jr. and R.M. Sweet. New York: Academic Press (1997).
d e s c r i p t i o n
X E N O N C H A M B E R

C A S E S T U D Y : X E N O N D E R I V A T I V E
& C R Y S T A L S T R U C T U R E
157
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A Xenon Derivative of Tt UvrB Crystal Prepared using the
Hampton Research Xenon Chamber
d e s c r i p t i o n
Thermus thermophilus UvrB (Tt UvrB), a 76 kDa protein is a com-
ponent of the nucleotide excision repair system. The Tt UvrB crystal
structures was determined by single isomorphous replacement /
anomalous scattering from a xenon derivative. The UvrB crystals
were pressurized with 500 psi of xenon gas for 15 minutes at room
temperature and flash cooled for data collection. Xenon derivatized
crystals diffracted to 1.9 angstrom.
Reference:
Machius, M., Henry, L., Palnitkar, M., and Deisenhofer, J. (1999) Proc. Natl. Acad. Sci USA, 96,
11717-11722.
Overall structure
of Tt UvrB with
the Xenon sites
(red spheres).
Environment of
Xenon Site 1
with Fo-Fc densi-
ty for the Xenon
atom contoured
at 10 .
Environment of
Xenon Site 2
with Fo-Fc densi-
ty for the Xenon
atom contoured
at 10 .
Environment of
Xenon Site 3
with Fo-Fc densi-
ty for the Xenon
atom contoured
at 10 .
Environment of
Xenon Site 4
with Fo-Fc densi-
ty for the Xenon
atom contoured
at 10 .
When using the Xenon Chamber to obtain deriva-
tized crystals, the gas pressure is typically between
200 and 500 psi. A typical 50 liter compressed
xenon gas cylinder has an initial pressure of 600
psi. Without some type of recovery system, if the
cylinder pressure is less than desired, the cylinder
cannot be used and the expensive xenon gas is
wasted. With the Xenon Recovery System connected
to a compressed xenon gas cylinder, pressures can
be increased by a factor of 9. This means a typical
compressed xenon gas cylinder is utilized at 95%
efficiency versus 50% efficiency without the recovery
system. The system easily connects to the Xenon
Chamber and Pressure Regulator. Contact Technical
Support for complete specifications.
Order Information
Cat. No. Name Description Price
HR4-797 Xenon Recovery System each $1,200.00
d e s c r i p t i o n
a p p l i c a t i o n
f e a t u r e s
n
Scavenge and recover xenon gas from low
pressure xenon gas cylinders
n
Utilize up to 95% of xenon gas in cylinders
X E N O N R E C O V E R Y S Y S T E M

l a b e l s & p e n s
Proteins crystals.
Kasumi Kobayashi, Taiji Nakae and Hiroyuki Akama. The Kitasato Institute, Kanagawa, Japan.
T A B L E O F C O N T E N T S
160 p e ns
160 - 161 t o u g h - t a g s

o n a r o l l
161 c r y o - b a b i e s

a n d c r y o - t a g s

o n a r o l l
162 t o u g h - s p o t s

o n a r o l l
163 l a s e r c r y o - b a b i e s

a n d c r y o - t a g s


163

l a s e r t o u g h - s p o t s

P A G E S
Designed for use with 0.2 ml microtubes, Tough-Tags On a Roll
- Teeny will withstand thermal-cycling, autoclaving, boiling water
baths and freezing to -40C.
Label Size: 0.81" x 0.28" (21 mm x 7 mm)
Color: White; Quantity: 1,500 labels per roll
d e s c r i p t i o n
T O U G H - T A G S

O N A R O L L - T E E N Y
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Labels for 0.2 ml microtubes
a p p l i c a t i o n
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Marking crystallization plates, labels,
sample tubes, and vials
n
Marking of labels and samples
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Fine point writing tip
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Black ink
n
Fast drying, permanent ink
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Red ink or black ink
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Pre-cut, peel off labels
n
Withstands solvents, incubation in high
humidity, and freezing to -40C
n
Works best with extra fine point markers
(Teeny Writer)
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Convenient, easy to dispense roll
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T O U G H - T A G S

O N A R O L L
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HR4-458 Tough-Tags

On a Roll - Teeny 1 roll $35.00


Order Information
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HR4-462 Teeny Writer each $6.00
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HR4-464 Solvent Resistant Pen - Black each $6.00
HR4-466 Solvent Resistant Pen - Red each $6.00
T E E N Y W R I T E R
S O L V E N T R E S I S T A N T P E N
The Teeny Writer has an extra fine tip. Recommended for writing on
all our labels for samples that will be stored at room temperature and
not exposed to chemicals and/or caustic agents. Ink dries in a matter
of seconds. The ink is NOT resistant to detergents and reagents used
in histology procedures. Color: Black.
The Solvent Resistant Pen is recommended for writing on Tough-
Tags. The ultra-permanent ink dries in a matter of seconds and
resists solvents such as xylene, alcohol, acetone, and formalin.
Testing is advised before use.
d e s c r i p t i o n
d e s c r i p t i o n
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T O U G H - T A G S

O N A R O L L
161
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HR4-460 Tough-Tags

On a Roll - Sidewall 1 roll $35.00


Tough-Tags On a Roll - Sidewall have been carefully engineered to
strongly adhere to all plastics and other materials. Sidewall Tough-
Tags withstand organic solvents, caustic agents, humid incubators,
boiling water baths, and freezer temperatures without peeling.
These pre-cut labels fit perfectly onto the sides of crystallization
plates allowing for visual identification of the individual plates in
a stack.
Label Size: 1.50" x 0.25" (38 mm x 6 mm)
Color: White; Quantity: 1,000 labels per roll
n
Labels for crystallization plates
a p p l i c a t i o n
T O U G H - T A G S

O N A R O L L S I D E WA L L
d e s c r i p t i o n
n
Withstands solvents, incubation in high
humidity, and freezing to -20C
n
Easy to peel off and affix
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Pre-cut labels fit perfectly on the side of
crystallization plates
f e a t u r e s
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Freezable in liquid nitrogen
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Chemically resistant
n
Made of a special smear-resistant material
that will accept any marking instrument
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Pre-cut labels on a roll in dispenser box
f e a t u r e s
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HR4-404 Cryo-Babies

On a Roll 1 roll $36.00


HR4-406 Cryo-Tags

On a Roll 1 roll $36.00


Labels for cryogenic storage. Withstand conventional and cryo-
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The labels will also withstand boiling water baths to 100C and dry
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metals. They resist detergents, oils, solvents, caustic agents, and
other challenges without peeling or falling off. Great for labeling
plastics and other materials for cryogenic storage. Cryo-Babies are
ideal for1.5-2.0 ml or larger tubes. Apply label to tube or container
BEFORE freezing.
Catalog #HR4-404 Label Size: 1.28" x 0.50" (33 mm x 13 mm)
Catalog #HR4-406 Label Size: 1.50" x 0.75" (38 mm x 19 mm)
Color: White; Quantity: 1,000 labels per roll
n
Labels for cryo labware cryogenic storage
a p p l i c a t i o n
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C R Y O - B A B I E S

A N D C R Y O - T A G S

O N A R O L L
T O U G H - S P O T S

O N A R O L L
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HR4-436 Tough-Spots

On a Roll - Small White - 1 roll $31.00


HR4-438 Tough-Spots

On a Roll - Small Red - 1 roll $31.00


HR4-440 Tough-Spots

On a Roll - Small Yellow - 1 roll $31.00


HR4-442 Tough-Spots

On a Roll - Small Green - 1 roll $31.00


HR4-444 Tough-Spots

On a Roll - Small Blue - 1 roll $31.00


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HR4-446 Tough-Spots

On a Roll - Large White - 1 roll $31.00


Tough-Spots On a Roll - Small are chemically inert, polyvinyl labels
that adhere to all plastics. They are 3/8" diameter circular labels
that are useful for tagging and easily identifying a wide range of
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colors.
Label Size: 3/8" diameter (9.5 mm diameter)
Quantity: 1,000 labels per roll
Tough-Spots On a Roll - Large are chemically inert, polyvinyl
labels that adhere to all plastics. They are 1/2" diameter circular
labels that are useful for tagging and easily identifying a wide
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Label Size: 1/2" diameter (13 mm diameter)
Color: White; Quantity: 1,000 labels per roll
T O U G H - S P O T S

O N A R O L L - S M A L L
T O U G H - S P O T S

O N A R O L L - L A R G E
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Labels for 1.5 - 2.0 ml tube tops
a p p l i c a t i o n
a p p l i c a t i o n
d e s c r i p t i o n
d e s c r i p t i o n
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For microtube cap or lid identification
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Freezable in liquid nitrogen
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Autoclavable and boilable
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Chemically resistant
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Won't dry out, fall off, or tear
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For microtube cap or lid identification
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Freezable in liquid nitrogen
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Chemically resistant
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Won't dry out, fall off, or tear
f e a t u r e s
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L A S E R C R Y O - B A B I E S

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163
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1 pack $70.00
Labels for cryogenic storage. Withstand conventional and cryogenic
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labels will also withstand boiling water baths to 100C and dry heat
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als. They resist detergents, oils, solvents, caustic agents, and other
challenges without peeling or falling off. Ideal for 1.5 to 2.0 ml or
larger tubes.
Label Size: 1.28 x 0.50" (33 mm x 13 mm)
Color: White; Quantity: 85 labels per sheet (1,700/pack)
Labels for cryogenic storage. Withstand conventional and cryogenic
storage (vapor and liquid phase nitrogen storage) to -196C. The
labels will also withstand boiling water baths to 100C and dry
heat up to 150C. Polyolefin labels adhere to all plastics, glass, and
metals. They resist detergents, oils, solvents, caustic agents, and
other challenges without peeling or falling off. Ideal for labeling
plastics and other materials for cryogenic storage.
Label Size: 1.50 x 0.75" (38 mm x 19 mm)
Color: White; Quantity: 60 labels per sheet (1,200/pack)
Tough-Spots Small are chemically inert, polyvinyl labels that adhere
to all plastics. They are 3/8" diameter circular labels that are useful
for tagging and easily identifying a wide range of small containers in
the crystallization lab.
Label Size: 3/8" diameter (9.5 mm diameter)
Color: White; Quantity: 192 labels per sheet (3,840/pack)
n
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L A S E R C R Y O - T A G S


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f e a t u r e s
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L A S E R T O U G H - S P O T S

Here is a recipe to try:


Mosaicity is about 0.5
t0 0.6
Reagent:
Crystal Screen Cryo
Reagent 23
Mix equal amounts of
Glucose Isomerase
and reagent. Vapor
diffusion method.

Mount crystal in
CryoLoop.

Mosaicity may be
a bit more in this
reagent and the
unit cell will shrink a
b o o k s
Proteins crystals.
Kasumi Kobayashi, Taiji Nakae and Hiroyuki Akama. The Kitasato Institute, Kanagawa, Japan.
T A B L E O F C O N T E N T S
166 m a c r o m o l e c u l a r c r y s t a l l o g r a p h y
166 m a c r o m o l e c u l a r c r y s t a l l o g r a p h y p r o t o c o l s ,
v o l u m e 1
167 p r o t e i n c r y s t a l l i z a t i o n s t r a t e g i e s f o r
s t r u c t u r a l g e n o m i c s
167 p r a c t i c a l p r o t e i n c r y s t a l l o g r a p h y
167 p r e p a r a t i o n a n d a n a l y s i s o f p r o t e i n c r y s t a l s
168 p r o t e i n m e t h o d s
168 m e t h o d s i n e n z y m o l o g y - p a r t c
168 m e t h o d s i n e n z y m o l o g y - p a r t d
169 c r y s t a l l o g r a p h y m a d e c r y s t a l c l e a r
169 o u t l i n e o f c r y s t a l l o g r a p h y f o r b i o l o g i s t s
169 i n t r o d u c t i o n t o m a c r o m o l e c u l a r c r y s t a l l o g r a p h y
170 p r i n c i p l e s o f p r o t e i n x - r a y c r y s t a l l o g r a p h y
170 m e m b r a n e p r o t e i n p u r i f i c a t i o n &
c r y s t a l l i z a t i o n , a p r a c t i c a l g u i d e
170 m e t h o d s a n d r e s u l t s i n c r y s t a l l i z a t i o n o f
m e m b r a n e p r o t e i n s
171 m a c r o m o l e c u l a r c r y s t a l l i z a t i o n m e t h o d s ,
v o l u m e 3 4 , n u m b e r 3
171 j o u r n a l o f s t r u c t u r a l b i o l o g y
172 p r e s e n t a t t h e f l o o d :
h o w s t r u c t u r a l m o l e c u l a r b i o l o g y c a m e a b o u t
172 c r y s t a l s a n d l i f e : a p e r s o n a l j o u r n e y
172 p r o t e i n c r y s t a l l o g r a p h y : a c o n c i s e g u i d e
173 p r o t e i n c r y s t a l l i z a t i o n
173 v i e w e r s
P A G E S
B O O K S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 166 Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
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Covers all aspects of protein crystallography. The 386 page text
is a practical handbook intended to be used by anyone who
wants to solve a structure by protein crystallography. This book
is divided into four chapters: (1) Laboratory Techniques, (2) Data
Collection Techniques, (3) Computational Techniques, and (4)
Protein Crystallography Cookbook.
P R A C T I C A L P R O T E I N C R Y S T A L L O G R A P H Y
n
Authors: Mark Sanderson and Jane Skelly
n
Publisher: Oxford University Press, 2007
n
ISBN-10: 0198520972
n
ISBN-13: 978-0198520979
n
Hardcover: 400 pages
d e s c r i p t i o n
Macromolecular Crystallography - conventional and high-through-
put methods
Macromolecular crystallography is the study of macromolecules
(proteins and nucleic acids) using x-ray crystallographic techniques
in order to determine their molecular structure. The knowledge of
accurate molecular structures is a pre-requisite for rational drug
design, and for structure-based function studies to aid the devel-
opment of effective therapeutic agents and drugs. The successful
determination of the complete genome (genetic sequence) of several species (including humans) has
recently directed scientific attention towards identifying the structure and function of the complete
complement of proteins that make up that species; a new and rapidly growing field of study called
'structural genomics'. There are now several important and well-funded global initiatives in operation
to identify all of the proteins of key model species. One of the main requirements for these initiatives
is a high-throughput crystallization facility to speed-up the protein identification process. The extent to
which these technologies have advanced, calls for an updated review of current crystallographic theory
and practice. This practical reference book features the latest conventional and high-throughput meth-
ods, and includes contributions from a team of internationally recognized leaders and experts. It will
be of relevance and use to graduate students, research scientists and professionals currently working
in the field of conventional and high-throughput macromolecular crystallography.
Author Information
Mark Sanderson is an experienced protein and nucleic-acid crystallographer and co-editor of Methods
in Molecular Biology, who has solved several key ab initio structures, notably nucleic-acid proteins.
Jane Skelly has widespread experience in the expression and crystallisation of macromolecules. Both
authors lecture undergraduates in structural biology.
M A C R O M O L E C U L A R C R Y S T A L L O G R A P H Y
d e s c r i p t i o n
Order Information
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HR5-224 Macromolecular Crystallography each $156.00
n
Author: Sylvie Doublie
n
Publisher: Humana Press, 2007
n
ISBN-10: 1588292924
n
ISBN-13: 978-1588292926
n
Hardcover: 312 pages
d e s c r i p t i o n
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Cat. No. Name Description Price
HR5-215 Macromolecular Crystallography Protocols, Vol. 1 each $40.00
Macromolecular Crystallography Protocols, now in two volumes,
examines major developments that have occurred since publica-
tion of the acclaimed first edition nearly a decade ago. Volume 1,
Preparation and Crystallization of Macromolecules and Volume 2,
Structure Determination, explore recent advances that have acceler-
ated the pace of structural determination and made crystallography
accessible to a broader range of investigators. Volume 1 is composed
of detailed protocols for the preparation and optimization of crystals,
including tips from the experts on the best methods for inducing proteins to adopt their crystalline form.
Volume 2 complements the first volume by addressing laboratory techniques for crystal handling and
structural characterization, as well as computational techniques for data collection, phasing, and refinement.
The volume concludes with a detailed and insightful survey of available crystallographic software. These
volumes will be an indispensable reference for obtaining macromolecular crystals and determining their
three-dimensional structure.
M A C R O M O L E C U L A R C R Y S T A L L O G R A P H Y P R O T O C O L S , V O L U M E 1
167
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Research advances in recent years have opened up the scope for the
development of new methods and tools to overcome the bottleneck
of protein crystallization. A variety of parameters that could previously
not be explored are now accessible thanks to sophisticated apparatus
and the development of new science-based techniques to monitor and
control the process of crystallization. However, in order to become
useful to the structural genomics effort, it is vital to miniaturize and
automate these techniques and adapt them to cope with the vast
numbers of leads resulting from the high-throughput screening procedures. Such efforts are those of the
immediate future and the focus of this book.
P R O T E I N C R Y S T A L L I Z A T I O N S T R A T E G I E S F O R S T R U C T U R A L G E N O M I C S
n
Editor: Naomi E. Chayen
n
Publisher: International University Line,
2007
n
ISBN-10: 097207743X
n
ISBN-13: 978-0972077439
n
Hardcover: 290 pages
d e s c r i p t i o n
P R O T E I N C R Y S T A L L I Z A T I O N T E C H N I Q U E S , S T R A T E G I E S , & T I P S
Order Information
Cat. No. Name Description Price
HR5-230 Protein Crystallization Strategies for Structural Genomics each $109.00
Covers all aspects of protein crystallography. The 386 page text
is a practical handbook intended to be used by anyone who
wants to solve a structure by protein crystallography. This book
is divided into four chapters: (1) Laboratory Techniques, (2) Data
Collection Techniques, (3) Computational Techniques, and (4)
Protein Crystallography Cookbook.
P R A C T I C A L P R O T E I N C R Y S T A L L O G R A P H Y
n
Author: Duncan E. McRee
n
Publisher: Academic Press; 2nd edition, 1999
n
ISBN-10: 0124860524
n
ISBN-13: 978-0124860520
n
Hardcover: 504 pages
d e s c r i p t i o n
Covers all aspects of protein crystallography. It is a practical
handbook intended to be used by anyone who wants to solve
a structure by protein crystallography. This book is divided into
four chapters: (1) Laboratory Techniques; (2) Data Collection
Techniques; (3) Computational Techniques; and (4) Protein
Crystallography Cookbook.
P R A C T I C A L P R O T E I N C R Y S T A L L O G R A P H Y
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR5-211 Practical Protein Crystallography each $142.00
This hard-to-find book is a classic. The first text written spe-
cifically for macromolecular crystallization. This twelve chapter
book provides an interface between the techniques and prac-
tices common to most biochemists and the procedures familiar
to x-ray diffractionists.
n
Author: Alexander McPherson
n
Publisher: Krieger Publishing, 1989
n
ISBN-10: 089464355X
n
ISBN-13: 978-0894643552
n
Hardcover: 384 pages
d e s c r i p t i o n
P R E P A R A T I O N A N D A N A L Y S I S O F P R O T E I N C R Y S T A L S
Order Information
Cat. No. Name Description Price
HR5-213 Preparation and Analysis of Protein Crystals each $75.00
B O O K S
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 168 Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
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P R O T E I N M E T H O D S
n
Authors: Daniel M. Bollag, Michael D.
Rozycki, & Stuart J. Edelstein
n
Publisher: Wiley-Liss Publishing; 2nd edition,
1996
n
ISBN-10: 0471118370
n
ISBN-13: 978-0471118374
n
Paperback: 415 pages
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR5-223 Protein Methods each $98.00
This text is a handy and practical guide of protein methods which
includes 36 pages dedicated to a straightforward, practical introduction
to macromolecular crystallization. Chapter titles include: Preparation
for Protein Isolation, Protein Extraction and Solubilization, Protein
Concentration Determination, Concentrating Protein Solutions, Gel
Electrophoresis, IEF, Immunoblotting, Ion Exchange, Affinity and Gel
Filtration Chromatography, and Hanging Drop Crystallization.
Macromolecular Crystallography, Part C (Methods in Enzymology,
Volume 368). Topics covered include: methodological methods in
crystals and data analysis.
M E T H O D S I N E N Z Y M O L O G Y - P A R T C
n
Editor: Charles W. Carter, Jr.
n
Publisher: Elsevier, 2003
n
ISBN-10: 0121822710
n
ISBN-13: 978-0121822712
n
Hardcover: 368 pages
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR5-210 Methods in Enzymology - Vol 368, Part C each $150.00
Macromolecular Crystallography, Part D (Methods in Enzymology,
Volume 374). Topics covered include: Phases, Map Interpretation
and Refinement, Analysis and Software.
M E T H O D S I N E N Z Y M O L O G Y - P A R T D
n
Editor: Charles W. Carter, Jr.
n
Publisher: Elsevier, 2003
n
ISBN-10: 0121827771
n
ISBN-13: 978-0121827779
n
Hardcover: 679 pages
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR5-212 Methods in Enzymology Volume 374, Part D each $150.00
169
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This book is an exciting resource for those who are vitally inter-
ested in macromolecular models derived from x-ray crystallography
but are unfamiliar with, or daunted by, crystallography itself. This
book is for readers who want an intellectually satisfying under-
standing of how three-dimensional models of proteins and nucleic
acids are derived from x-ray diffraction analysis of crystals. This
understanding is essential for wise use of crystallographic models
in studying enzyme catalysis, protein-mediated transport and
recognition, protein/nucleic acid interaction, and protein folding.
It is also vital in interpreting data from chemical, kinetic, thermodynamic, and spectroscopic studies
of macromolecules.
C R Y S T A L L O G R A P H Y M A D E C R Y S T A L C L E A R
n
Author: Gale Rhodes
n
Publisher: Academic Press; 3rd edition, 2006
n
ISBN-10: 0125870736
n
ISBN-13: 978-0125870733
n
Paperback: 352 pages
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR5-233 Crystallography Made Crystal Clear each $63.00
P R A C T I C A L P R O T E I N C R Y S T A L L O G R A P H Y
d e s c r i p t i o n
Outline of Crystallography for Biologists is intended for researchers
and students in the biological sciences who require an insight into
the methods of x-ray crystallography without needing to learn all the
relevant theory. The main text is purely descriptive and is readable
by those with minimal mathematical knowledge. Some mathematical
detail is given throughout in boxes, but these can be ignored. Theory
is limited to the essentials required to comprehend issues of quality.
There is an extensive reference section and suggestions for further
reading for those who wish to delve deeper. The first part, 'Fundamentals', presents the underlying ideas
which allow x-ray structure analysis to be carried out and provides an appropriate background to courses
in structural determination. The second part, 'Practice', gives more information about the procedures
employed in the course of crystal structure determination. The emphasis is on the quality measures of
x-ray diffraction analysis to give the reader a critical insight into the quality and accuracy of a structure
determination and to enable the reader to appreciate which parts of a structure determination may have
caused special difficulty. There is no pretence of completeness and many matters discussed in standard
crystallography texts are deliberately omitted. However, issues not brought out in the standard texts are
discussed, making it a useful resource for non-practicing crystallographers as well.
n
Author: David Blow
n
Publisher: Oxford University Press, 2002
n
ISBN-10: 0198510519
n
ISBN-13: 978-0198510512
n
Paperback: 248 pages
Order Information
Cat. No. Name Description Price
HR8-086 Outline of Crystallography for Biologists each $82.00
O U T L I N E O F C R Y S T A L L O G R A P H Y F O R B I O L O G I S T S
n
Author: Alexander McPherson
n
Publisher: Wiley-Blackwell; 2nd edition,
2009
n
ISBN-10: 0470185902
n
ISBN-13: 978-0470185902
n
Paperback: 267 pages
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR8-062 Introduction to Macromolecular Crystallography each $80.00
This book provides a comprehensive, approachable summary of the
field of crystallography, from the fundamental theory of diffraction
and properties of crystals to applications in determining macromo-
lecular structure.
Dedicated to providing a complete introduction to the subject
that does not assume a background in physics or math, the book's
contents flow logically from basic principles to key methods, such as
those for solving phase problems, interpretation of Patterson maps,
and the difference Fourier method. The Introduction includes:
Practical Instruction on the Interpretation of Data and Methods for Determining Phases.
I N T R O D U C T I O N T O M A C R O M O L E C U L A R C R Y S T A L L O G R A P H Y
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 170 Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
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B O O K S
M E M B R A N E P R O T E I N P U R I F I C AT I O N & C R Y S TA L L I Z AT I O N , A P R A C T I C A L G U I D E
Order Information
Cat. No. Name Description Price
HR5-241 Membrane Protein Purification and Crystallization each $98.00
This guide presents isolation and crystallization techniques in a
concise form, emphasizing the critical aspects unique to membrane
proteins. It explains the principles of the methods and provides
protocols of general use, permitting researchers and students new
to this area to adapt these techniques to their particular needs. Key
features: Provides general guidelines and strategies for isolation
and crystallization of membrane proteins. Gives detailed protocols
that have wide application, and low specialized equipment needs.
Emphasizes recent progress in production and purification of recombinant membrane proteins, espe-
cially of histidine-tagged and other affinity-epitope-tagged proteins. Summarizes recent developments of
Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombi-
nant techniques, and is especially suited for proteomic analyses of membrane protein complexes. Gives
detailed protocols for membrane protein crystallization, and describes the production and use of anti-
body fragments for high resolution crystallization. Presents a comprehensive guide to 2D-crystallization
of membrane proteins.
n
Editors: Carola Hunte, Gebhard Von
Jagow, Hermann Schagger
n
Publisher: Academic Press;
2nd edition, 2002
n
ISBN-10: 0123617766
n
ISBN-13: 978-0123617767
n
Spiral-bound: 316 pages
d e s c r i p t i o n
P R I N C I P L E S O F P R O T E I N X - R A Y C R Y S T A L L O G R A P H Y
n
Author: Jan Drenth
n
Publisher: Springer; 3rd edition, 2006
n
ISBN-10: 0387333347
n
ISBN-13: 978-0387333342
n
Hardcover: 332 pages
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR5-219 Principles of Protein X-Ray Crystallography each $90.00
Strong on crystallography, light on crystal growing (17 pages).
Fifteen chapters including Crystallizing a Protein, X-Ray Sources
and Detectors, Crystals, Theory of X-Ray Diffraction By a Crystal,
Average Reflection Intensity and Distribution of Structure Factor
Data, Special Forms of the Structure Factor, Phase Improvement,
The Solution of the Phase Problem by the Isomorphous Replacement
Method, Anamolous Scattering, Molecular Replacement, Direct
Methods, Refinement, Combination of Phase Information, Phase
Information from Partial Structure Data, Solvent Flattening, and
Molecular Averaging.
M E T H O D S A N D R E S U LT S I N C R Y S T A L L I Z AT I O N O F M E M B R A N E P R O T E I N S
n
Editor: So Iwata
n
Publisher: International University Line,
2002
n
ISBN-10: 0963681796
n
ISBN-13: 978-0963681799
n
Hardcover: 380 pages
d e s c r i p t i o n
This text consists of four parts. Parts 1 and 2 include an introduc-
tion as well as general principles and techniques in membrane pro-
tein crystallization. This is followed by chapters covering the use of
detergents, crystallization in lipidic cubic phases, the use and gen-
eration of antibody fragments, porin as a model, and crystallization
of membrane proteins in oils. Part 3 focuses on specific examples
of membrane proteins whose structures have been solved. Part 4
covers the design of a kit for membrane protein crystallization.
Order Information
Cat. No. Name Description Price
HR5-237 Methods and Results in Crystallization of Membrane Proteins each $109.00
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This is a single issue of Methods, a companion journal to Methods
in Enzymology, focusing on rapidly developing techniques. This
single issue is dedicated to Macromolecular Crystallization and be-
gins with an Introduction to Protein Crystallization by Alexander
McPherson. The issue features the following additional thirteen
articles. Protein crystallization and phase diagrams, Neer Asherie.
Growth and disorder of macromolecular crystals: insights from
atomic force microscopy and x-ray diffraction studies, Alexander J.
Malkin and Robert E. Thorne. Ions from the Hofmeister series and
osmolytes: effects on proteins in solution and in the crystallization process, Kim D. Collins. Effects of
naturally occurring osmolytes on protein stability and solubility; issues important in protein crystalliza-
tion, D.W. Bolen. Practical aspects of using the microbatch method in screening conditions for protein
crystallization, Allan DArcy, Aengus Mac Sweeney, and Alexander Haber. Automated systems for protein
crystallization, Joel Bard, Kimberly Ercolani, Kristine Svenson, Andrea Olland, and Will Somers. Lipidic
cubic phases as matrices for membrane protein crystallization, Peter Nollert. The use of recombinant
and molecular engineering in protein crystallization, Zygmunt Derewenda. A pedestrian guide to mem-
brane protein crystallization, Michael Wiener. Crystallization data mining in structural genomics: using
positive and negative results to optimize protein crystallization screens, Rebecca Page and Raymond C.
Stevens. Predictive models for protein crystallization, Bernhard Rupp and Junwen Wang. Crystallization
of RNA and RNA-protein complexes, Ailong Ke and Jennifer A. Doudna. Macromolecular cryocrystallog-
raphy methods for cooling and mounting protein crystals are cryogenic temperatures, J.W. Pfugrath.
n
Edited by: Alexander McPherson
n
Publisher: Elsevier; Methods, Volume 34,
Number 3, November 2004
n
ISSN: 1046-2023
n
Paperback: 172 pages
Order Information
Cat. No. Name Description Price
HR8-061 Macromolecular Crystallization each $40.00
d e s c r i p t i o n
M A C R O M O L E C U L A R C R Y S T A L L I Z A T I O N M E T H O D S , V O L U M E 3 4 , N U M B E R 3
This special issue of the Journal of Structural Biology focuses on
Macromolecular Crystallization in the Structural Genomics Era.
Following an Introduction by Alexander McPherson, there are 19
regular articles focused on the crystallization of biological macro-
molecules. If crystallization is your thing, you need this. Thanks to
everyone contributing to this special issue. Well done everyone!
J O U R N A L O F S T R U C T U R A L B I O L O G Y
n
Editor: Alexander McPherson
n
Publisher: Academic Press; Volume 142,
Number 1, April 2003
n
ISSN: 1047-8477
n
Paperback: 231 pages
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR8-100 Journal of Structural Biology each $60.00
Crystal image.

Sylvia Luckner, Lehrstuhl fr Biotechnik,
University Erlangen-Nrnberg Germany.
B O O K S
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Order Information
Cat. No. Name Description Price
HR5-214 Present at the Flood each $35.00
This book chronicles a revolution in molecular biologythe crucial
30 years (roughly between 1933 and 1963) during which our ideas
about proteins and nucleic acids changed from those of formless,
functionless organic chemicals into precisely structured molecular
machines with specifc biological purpose. Proteins evolved from
being colloidal micelles or globules with no specifc structure (or
even sequence) into quite precisely structured molecular catalysts,
carrier proteins, and information-sensing agents. Indeed, the very
idea that the amino acids of a protein were linked in a specifc
order in long linear chains was not accepted initially. During this same time period, DNA changed
from being a sterile repeating polymer of no particular function (the tetranucleotide hypothesis) into a
double helix that serves as the archive of genetic information.
n
Author: Richard Dickerson
n
Publisher: Sinauer Associates, 2005
n
ISBN-10: 0878931686
n
ISBN-13: 978-0878931682
n
Paperback: 307 pages
d e s c r i p t i o n
PRESENT AT T HE F L OOD: HOW ST RUCT URAL MOL ECUL AR BI OL OGY CAME ABOUT
n
Author: Cele Abad-Zapatero
n
Publisher: International University Line,
2002
n
ISBN-10: 0972077405
n
ISBN-13: 978-0972077408
n
Paperback: 300 pages
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR5-239 Crystals and Life: A Personal Journey each $15.00
This book introduces crystallography, the field, and applications
of structural biology using metaphors from the arts, music,
poetry, and architecture. The reader will find a tapestry filled
by personal experiences, historical anecdotes, biographical
snapshots of scientific heroes of the field, and crystallographic
concepts illustrated with artistic analogies. All these elements
create a concoction that makes crystallography accessible,
comprehensible, intriguing, inspiring, and beautiful. The book
is divided in sections covering the following: the basic elements
of crystallography, novel technologies, practical applications and future perspectives. Since crystal-
lography is quintessentially a visual science, the illustrations play a very important role in providing
an excellent set of landmarks to guide the reader on this personal journey of discovery.
C R Y S T A L S A N D L I F E : A P E R S O N A L J O U R N E Y
n
Authors: Eaton E. Lattman and
Patrick J. Loll
n
Publisher: The Johns Hopkins University
Press, 2008
n
ISBN-10: 0801888085
n
ISBN-13: 978-0801888085
n
Paperback: 152 pages
d e s c r i p t i o n
Order Information
Cat. No. Name Description Price
HR5-221 Protein Crystallography: A Concise Guide each $37.00
The proteome remains a mysterious realm. Researchers have
determined the structures of only a small fraction of the proteins
encoded by the human genome. Crystallography continues to
be the primary method used to determine the structures of the
remaining unknown proteins. This imaging technique uses the
diffraction of x-rays to determine a proteins three-dimensional
molecular structure. Drawing on years of research and teach-
ing experience, Eaton E. Lattman and Patrick J. Loll use clear
examples and abundant illustrations to provide a concise and accessible primer on protein crystal-
lography. Discussing the basics of diffraction, the behavior of two- and three-dimensional crystals,
phase determination (including MIR and MAD phasing and molecular replacement), the Patterson
function, and refinement, Lattman and Loll provide a complete overview of this important tech-
nique, illuminated by physical insights. The crisp writing style and simple illustrations will provide
beginner crystallographers with a guide to the process of unraveling protein structure.
P R O T E I N C R Y S T A L L O G R A P H Y : A C O N C I S E G U I D E
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
Order Information
Cat. No. Name Description Price
HR5-231 Protein Crystallization each $99.00
Completely revised and updated, Protein Crystallization, 2nd Edi-
tion is a greatly expanded follow-up to the best-selling 1st edition.
Completely new chapters on high-throughput methods, mass
spectrometry, microcalorimetry, counterdiffusion, heavy-atom
derivatization, selenomethionine-labeling, rational strategies for
crystallization, and protein modifcation to improve crystallization.
Updated chapters on formulation of the protein before crystalliza-
tion, characterization of the protein by dynamic light scattering,
classic methods and the phase diagram, seeding, and cryoprotection of the crystals. Thirty full-color
plates for evaluating crystallization drops. Separate section of laboratory exercises, ideal for crystalliza-
tion courses. A-Z glossary.
n
Editor: Terese Bergfors
n
Publisher: International University Line;
2nd edition, 2009
n
ISBN-10: 0972077448
n
ISBN-13: 978-0972077446
n
Hardcover: 474 pages
n
Editor: Terese Bergfors
n
Folding 3-D viewer
n
Editor: Terese Bergfors
n
3-D stereo glasses with folding wire legs
for compact storage
d e s c r i p t i o n
PROT EI N CRY STAL L I Z AT I ON
P R A C T I C A L P R O T E I N C R Y S T A L L O G R A P H Y
d e s c r i p t i o n
The Stereopticon is punched from black plastic and folds
completely flat to fit inside a book. The Stereopticon uses plastic
lenses.
S T E R E O P T I C O N S T E R E O V I E W E R
P R A C T I C A L P R O T E I N C R Y S T A L L O G R A P H Y
d e s c r i p t i o n
The Stereo Viewer features an adjustable nose bridge. It is made
of high impact, crystal-clear plastic with a 2.2 magnification, 4.7
focal length and adjustable inter-pupillary distance.
S T E R E O V I E W E R
Order Information
Cat. No. Name Description Price
HR4-517 Stereo Viewer each $12.00
173
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Order Information
Cat. No. Name Description Price
HR4-513 Stereopticon each $9.00
HR4-515 Stereopticon 10 pack $81.00
f e a t u r e s
f e a t u r e s
p r o t e i n c r y s t a l l i z a t i o n s t a n d a r d s
Protein crystal.
Alexey Rak, Max-Planck-Institut fur Molekulare Physiologie, Department of Physical Biochemistry, Dortmund, Germany.
T A B L E O F C O N T E N T S
P A G E S
176 g l u c o s e i s o m e r a s e
177 l y s o z y m e
178 x y l a n a s e
179 l i p a s e b
n
Crystallization grade protein standard
n
Protein standard for crystallization studies and
applications
n
Crystallization training and student demos
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 176
a p p l i c a t i o n
f e a t u r e s
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s
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O
HOCH
2
H
HO
OH
H
H
OH
H
OH
O
HOCH
2
OH
H HO
H
OH
HOCH
2
H
+
O
HOCH
2
H
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H
H
OH
H
OH
Glucose Glucose Fructose
Glucose Isomerase catalyzes the isomerization
reaction shown. The reaction is reversible and
goes to equilibrium, approximately equal con-
centrations of fructose and glucose.
d e s c r i p t i o n
Glucose Isomerase can be crystallized by sitting, hanging or sand-
wich drop vapor diffusion, batch, liquid/liquid diffusion, pH shift,
temperature jump, as well as by dialysis. It can be crystallized rapidly
across a broad range of screen conditions which makes the enzyme
an excellent candidate for crystallization studies and demonstrations.
Glucose Isomerase is supplied as a crystal suspension in the follow-
ing medium: Approximately 33 mg/ml in 6 mM TRIS hydrochloride
pH 7.0, 0.91 M Ammonium sulfate, 1 mM Magnesium sulfate.
Glucose Isomerase is prepared by Macrocrystal Oy, exclusively for Hampton Research.
References
1. Carrell, H. L., et al., Proc. Natl. Acad. Sci. USA (1989) Vol 86, 4440-4444.
2. Glucose Isomerase from Streptomyces rubiginosus - potential molecular weight standard for small-angle x-ray scattering. Maciej Kozak. J. Appl. Cryst.
(2005). 38, 555-558.
Order Information
Cat. No. Name Description Price
HR7-100 Glucose Isomerase 1 g $284.00
HR7-102 Glucose Isomerase 100 mg $60.00
G L U C O S E I S O M E R A S E
Glucose Isomerase Crystals
n
Crystallization grade protein standard
n
Protein standard for crystallization studies
and applications
n
Crystallization training and student demos
n
Hen egg white source
a p p l i c a t i o n
f e a t u r e s
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d e s c r i p t i o n
A very cost-effective and convenient crystallization grade lysozyme
(hen egg white). The Lysozyme Kit is a convenient buffer and
lysozyme set for teaching, demonstrations or instrumentation setup
and validation procedures. The kit features 12 vials with 20 mg of
lysozyme and 12 vials of 0.02 M Sodium acetate trihydrate pH 4.6
buffer. To prepare the lysozyme for crystallization, simply add the
supplied buffer to the protein. No weighing, no messing with fluffy
crystalline powder, no mess, and no variability. Each tube can make
up to 1 ml of a 20 mg/ml stock or 200 l of a 100 mg/ml stock. Twelve
sets of tubes and a stable shelf life make this a handy kit to keep in the
fridge. Lysozyme is available separately in an 8 g vial of lysozyme pow-
der. To dissolve the powder, use the Lysozyme Crystallization Buffer.
For teaching and demonstrations requiring crystals in a short period
of time, one should consider the 15-Minute Lysozyme Crystallization
Reagent (30% w/v Polyethylene glycol monomethyl ether 5,000, 1.0 M
Sodium chloride, 0.05 M Sodium acetate trihydrate pH 4.6). For flex-
ibility, one can also use the Lysozyme Crystallization Buffer stock (1.0
M Sodium acetate trihydrate pH 4.6) or the StockOptions Sodium
Acetate buffer kit and 5.0 M Sodium chloride to create custom grid
screens and practicals for demonstrating and teaching the fundamen-
tals of optimization of sample concentration and reagent concentration. The Grid Screen Sodium Chloride
kit is a convenient kit for demonstrating the effect of varying pH and reagent concentration on the solubility
and crystallization of a protein.
Order Information
Cat. No. Name Description Price
HR7-108 Lysozyme Kit 12 x 20 mg plus $60.00
12 x 1 ml solubilization buffer
HR7-110 Lysozyme 8 g $60.00
HR2-731 Lysozyme 1.0 M Sodium acetate trihydrate $29.00
Crystallization Buffer pH 4.6, 100 ml
HR2-805 15 Minute Lysozyme 30% w/v PEG MME 5,000, 1.0 M Sodium $39.00
Crystallization Reagent chloride, 0.05 M Sodium acetate trihydrate
pH 4.6, 100 ml

HR2-637 Sodium Chloride for 5.0 M Sodium chloride, 200 ml $33.00
Lysozyme Crystallization
HR2-233 StockOptions Sodium 1.0 M Sodium acetate trihydrate $195.00
Acetate kit pH 3.6 to 5.6, 21 reagents

HR2-219 Grid Screen Sodium chloride versus pH crystallization $175.00
Sodium Chloride kit screen kit
Lysozyme crystals grown using the Hampton Research Silver Bullets screen
L Y S O Z Y M E
n
Crystallization grade protein standard
n
Protein standard for crystallization studies and
applications
n
Crystallization training and student demos
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 178
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Xylanase can be crystallized by sitting, hanging or sandwich drop
vapor diffusion, batch, liquid/liquid diffusion, pH shift, temperature
jump, as well as by dialysis. It can be crystallized rapidly across a
broad range of screen conditions which makes the enzyme an excel-
lent candidate for crystallization studies and demonstrations. It is
supplied as a solution, produced by dissolving pure xylanase crystals
with phosphate buffer and glycerol. The solution is filtered with a
0.2 micron filter and has the final formulation:
Xylanase Concentration: 36 mg/ml
Absorbance at 280 nm: 97
Activity: 648,000 nkat/ml
Glycerol Concentration: 43% w/v
Na/K phosphate: 0.18 M
pH: 7
Specific Gravity: 1.124 g/ml
Xylanase is prepared by Macrocrystal Oy, exclusively for Hampton Research.
References
1. Torronen, A., et al., The EMBO Journal (1994) Vol 13, No 11, 2493-2501.
2. Torronen, A. ,et al., J. Mo. Biol. (1993) Vol 233, 313-316.
3. Crystals of family 11 xylanase II from Trichoderma longibrachiatum that diffract to atomic resolution. Natalia Moiseeva and Marc Allaire. Acta Cryst. (2004).
D60, 1275-1277.
4. Structures of an orthorhombic form of xylanase II from Trichoderma reesei and analysis of thermal displacement. Watanabe et al. Acta Cryst. (2006). D62,
784-792.
5. Cryogenic (<20 K) helium cooling mitigates radiation damage to protein crystals. B. Leif Hanson et al. Acta Cryst (2007) D63, 486-492.
Order Information
Cat. No. Name Description Price
HR7-104 Xylanase 1 g $463.00
HR7-106 Xylanase 100 mg $69.00
X Y L A N A S E
Protein crystals grown using the Silver Bullets screen from
Hampton Research.
Bob Cudney & Peter Nguyen, Hampton Research; Alex McPherson,
University of California, Irvine.
Xylanase Crystals
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Protein crystals grown using the Silver Bullets screen from
Hampton Research.
Bob Cudney & Peter Nguyen, Hampton Research; Alex McPherson,
University of California, Irvine.
Lipase B Crystals
Lipase B Crystals Grown by Macrocrystal Oy
n
Protein standard for crystallization studies
and applications
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Crystallization training and student demos
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d e s c r i p t i o n
Lipase B is a pure, dried, crystalline form of Lipase B from
Candida antarctica produced by submerged fermentation of
a genetically modified Aspergillus oryzae microorganism. It is
a white crystal powder where Lipase B is present as crystals
and there are no other ingredients or buffering salts.
Lipases (EC 3.1.1.3) make up a diverse group of water
soluble enzymes that catalyze the hydrolysis of ester bonds in waterinsoluble, lipid substrates. Lipases
are characterized by their drastically increased esterase activity when adsorbed to a lipid surface (interfacial
activation). Lipases are a form of esterases. They perform essential roles in the digestion, transport, and
processing of dietary lipids such as triglycerides, fats, oils in most, if not all, living organisms.
Chemical name, synonym Triacylglycerol hydrolase EC 3.1.1.3
M
r
35 kDa
pH 5.0 - 7.0
Isoelectric point 6.0
Appearance: White crystal powder
References
1. Crystallographic and molecular-modeling studies of lipase B from Candida antarctica reveal a stereospecifi city pocket for secondary alcohols. Uppenberg, J.,
Ohrner, N., Norin, M., Hult, K., Kleywegt, G.J., Patkar, S., Waagen, V., Anthonsen, T., Jones, T.A. Biochemistry v34 pp. 16838-51, 1995.
2. The sequence, crystal structure determination and refi nement of two crystal forms of lipase B from Candida antarctica. Uppenberg, J., Hansen, M.T., Patkar,
S., Jones, T.A. Structure v2 pp. 293-308, 1994.
3. Crystallization and preliminary X-ray studies of lipase B from Candida antarctica. Uppenberg J., Patkar S.; Bergfors T., Jones, T. A.. Journal of Molecular
Biology, 1994, vol. 235, no2, pp. 790-792.
Order Information
Cat. No. Name Description Price
HR7-099 Lipase B 100 mg $184.00
L I P A S E B
c r y s t a l g r o w t h 1 0 1
Protein crystals.
Kimberly J. Skinner, Structural Biology and Biophysics, Pfizer Global R&D, Sandwich, Kent, United Kingdom.
T A B L E O F C O N T E N T S
P A G E S
182 - 183 p r e l i m i n a r y s a m p l e p r e p a r a t i o n
184 - 185 c r y s t a l g r o w t h t e c h n i q u e s
186 h a n g i n g d r o p v a p o r d i f f u s i o n c r y s t a l l i z a t i o n

187 - 189 s i t t i n g d r o p v a p o r d i f f u s i o n c r y s t a l l i z a t i o n
190 c r y s t a l l i z a t i o n u n d e r o i l
191 - 192 d i a l y s i s c r y s t a l l i z a t i o n
193 v i e w i n g c r y s t a l l i z a t i o n e x p e r i m e n t s
194 b i r e f r i n g e n c e
195 - 196 s a l t o r b i o l o g i c a l c r y s t a l s ?
197
p h v e r s u s n u m b e r o f c r y s t a l s
198
p h p o i n t s t o p o n d e r
198 c r y s t a l g r o w t h v i a p h r e l a x a t i o n
199 p r o t e i n c o n c e n t r a t i o n v e r s u s n u m b e r o f c r y s t a l s

200 - 201 c r y s t a l l i z a t i o n p o l l i n g b o o t h
202 c r y o q u i c k i e s
203 h o w t o : c r y o c r y s t a l l o g r a p h y a n d t h e c r y s t a l c a p s y s t e m
204 u s i n g h a l i d e s f o r p h a s i n g m o l e c u l a r s t r u c t u r e s
205 m i c r o s e e d i n g

206 d e t e r g e n t s a t w o r k
207 - 208 p r o t e i n c r y s t a l l i z a t i o n r e c i p e s
209 - 210 t e m p e r a t u r e a s a c r y s t a l l i z a t i o n v a r i a b l e
212 - 219 t i p s f r o m r a m c
220 - 234 c r y s t a l l i z a t i o n t i p s a - z
235 - 263 c r y s t a l l i z a t i o n t i p s
264 - 265 c r y s t a l l i z a t i o n b u f f e r s t a b l e

266 - 267 s o l u b i l i t y t a b l e
preli mi nary sample preparati on
Lyophilization
Avoid lyophilization. Even though there are many examples of proteins which crys-
tallize after lyophilization (lysozyme, thaumatin, hemoglobin, etc.), lyophilization is
to be avoided when possible. If the protein is lyophilized, it needs to be dialyzed
before crystallization. Dialyze the protein against deionized water or a stabilization
buffer before crystallization. Dialysis will remove non-volatile buffers and other
chemicals that may have been present before lyophilization.
Ammonium Sulfate Precipitation
Avoid using Ammonium sulfate precipitation as a final purification and/or con-
centration step. It is often very difficult to completely remove all the Ammonium
sulfate by a desalting column of dialysis. The remaining trace amounts can inter-
fere with crystallization screening results and create reproducibility problems.
It is not uncommon for trace amounts of Ammonium sulfate in the sample
to cause precipitation or excessive nucleation in screen conditions containing
polyethylene glycol and salt.
Batches
Avoid combining different purification batches for crystallization trials.
Purification conditions and procedures are never identical so each batch should
be screened separately.
Profile the Protein
Ideally, you will purify your own protein, but this is not always reality. So, it is
always a good idea to characterize your protein before beginning crystallization
experiments. Profiling your protein before crystallization can often provide valu-
able clues during screening and optimization of crystallization conditions. Assay
to seriously consider:
SDS-PAGE
Native PAGE or Dynamic Light Scattering
IEF (Isoelectric Focusing) Gel
Mass Spectroscopy
The results of these assays can:
Determine the purity of the sample
Determine the homogeneity of the sample
Identify batch to batch variations
Identify stability problems with the sample
How Pure?
How pure should the protein sample be for crystallization trials? As pure as
possible. That's some answer, is it not? Integrating common sense into the ques-
tion, we might arrive at the following answer. For initial screening, the sample
should be at least 90 to 95% pure on a Coomassie stained SDS-PAGE. Finally, it
does no harm to screen an impure sample as one can always perform further
purification. Remember, crystallization used to be considered a very powerful
purification tool (and still is!).
If the initial screen does not produce crystals, any promising results, or it
becomes next to impossible to improve crystal quality during optimization, one
should consider further purification of the sample.
Storing the Sample
Most proteins can be stored successfully at 4C or -70C.
Check with the person preparing the protein or compare your protein to a simi-
lar protein in the literature for best storage temperature.
Ideally, one should assay the activity and stability of the protein before storage and
then later on at various points in time to determine the sample storage stability.
Repeated freezing and thawing of the sample should be avoided. Aliquot the
sample into multiple small microcentrifuge tubes. Make the aliquots small enough
so that the entire aliquot can be consumed in the experiment after thawing.
Sometimes people like to add Glycerol (10 to 50% v/v) to help proteins bet-
ter tolerate freezing. Avoid this if possible since it is often difficult to remove
Glycerol by dialysis or filtration. The presence of Glycerol is a crystallization vari-
able. It can behave as a precipitant, an additive, or cryoprotectant and therefore
can influence the outcome of a crystallization experiment.
In general, it is better to store proteins more concentrated than diluted. When
too dilute, adsorption of the protein onto the storage container can lead to
significant losses. However, precipitation can sometimes be a problem when the
protein is stored too concentrated.
Sample Handling
Be nice to your protein. Remember that proteins make an excellent food source
for microbes. Protect your sample from microbes by storing the protein at less
than 4C and not leaving it for extended periods of time at room temperature.
When thawing a sample or mixing a lyophilized sample into solution, do not
shake or vortex the protein. Avoid foaming the sample. Foam can be a sign of
denaturation.
Allow the sample to equilibrate to the temperature where the crystallization
experiments will be set up and/or incubated before setting the experiment.
The field of crystal growth is full of opinions and controversy. There are several
opinions on what should be done with the sample just prior to setting the crystal-
lization experiment. Let's have a look at those opinions.
Some like to filter the sample through a 0.2 micron (or smaller, but be sure
to compare the MW of your sample to the pore size of your filter so as not to
stick your sample on the filter) pore size filter into a sterile container. Filtration
can remove microbial contamination (but not the proteases) as well as sample
aggregation. Turbid sample solutions with lots of precipitate should be solu-
bilized or centrifuged before filtration to avoid the ugly experience of sticking
the sample to a filter membrane. Use filters with the smallest possible dead
volume to minimize sample loss. Some of the centrifugal microfiltration devices
are certainly worth consideration. Before filtering the sample, wash or flush the
filter with a small amount of the sample buffer/storage solution. This will test
the filter for compatibility with your sample buffer and remove any trace glycerol
which can sometimes be present from the manufacturer. If possible, test filter
a small aliquot of the sample, and measure the activity/OD before filtering the
entire sample. Do this to test the adherence of the sample to the filter media.
Read and follow the instructions supplied with the filter before introducing the
sample to the filter.
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 182 Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
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P A G E 1 - 2
Some like to centrifuge the sample. Centrifugation removes large sample aggre-
gates and amorphous debris. Post centrifugation views can provide a visual clue
of aggregation/precipitate for seemingly clear solutions. Following centrifugation,
use only the supernatant for crystallization trials.
Others prefer to avoid filtration or centrifugation before setting crystallization
experiments. One view is that the presence of amorphous material or aggregates
can enhance the changes for crystallization by acting as nucleants.
To Azide or Not
Sodium azide (NaN
3
) is an anti-microbial preservative that is sometimes used to pro-
tect samples and crystallization reagents from microbial contamination. Sodium azide
is toxic and should be handled with care. Typical Sodium azide concentrations are 1
mM or if you prefer % measurements, between 0.02% and 0.1% w/v.
If you choose to use Sodium azide remember that:
It is toxic to humans as well as microbes.
It is an inhibitor for some proteins and may become an unintentional ligand
for your sample.
It can interfere with heavy atom derivatization.
Some metal azides are explosive.
There are reports where eliminating Sodium azide from the experiment
improved crystallization.
Alternatives to Sodium Azide include Thymol & Thimerosal.
A final alternative to the use of antimicrobials is the use of proper sterile tech-
nique and materials. Sterile filter all samples and reagents into sterile containers.
Store samples and reagents at 4C or lower. Use sterile pipet tips. Keep your
work area clean. Develop a sterile technique with your crystallization setups.
With common sense, sterile reagents and sample, good technique, and sterile
pipet tips, one can successfully avoid the use of chemical antimicrobials in the
crystallization lab.
Label & Organize Samples
Label samples clearly with the sample identification, batch identification, and date
of storage. Small cryo labels can be very useful here. Color coding samples can
be a nice organization tool. For the sake of easy organization and identification,
it is sometimes more convenient to nest samples. For example, store batches of
small microcentrifuge tubes in 10 ml or 50 ml centrifuge tubes and organize them
by batch or sample.
It is prudent to write down and hold onto detailed notes concerning the puri-
fication, storage, and handling of the sample. It is obvious that one should also
maintain records of crystallization trials which should include:
Sample information
Name of sample
Sample identification (batch, storage location, storage temperature, etc.)
Sample buffer composition, additives, ligands, etc.
Sample concentration
Crystallization experiment information
Method
Drop size and composition
Reagent composition
Temperature
Date
Name of person performing experiment
Questions to Ponder about the Sample
Does a similar sample exist and has it been crystallized?
Does the sample contain free cysteines?
Does the sample contain additives such as Sodium azide, ligands, inhibitors, or
substrates?
Is the protein glycosylated?
Is the protein phosphorylated?
Is the protein N-terminal methylated?
At what temperature is the protein stable?
How does sample solubility and stability change temperature?
How does sample solubility and stability change with pH?
Does the sample bind metals?
Is the protein sensitive to proteolysis?
What class of protein am I working with (antibody, virus, enzyme, membrane
protein)?
What have been the most successful approaches with my class of protein?
What is the source of the sample?
How was the sample purified and stored before it arrived into my hands?
What is in the sample container besides the sample (buffer, additives, etc.)?
Is the sample pooled purification aliquots or a single batch?
How much sample do I have and how much more is available?
How pure is the sample?
How homogeneous is the sample?
Does anyone possess any solubility information on this sample?
What is unique about this protein?
What is necessary chemically and physically to maintain a stable, active sample?
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1991-2009 Hampton Research Corp. All rights reserved. Printed in the United States of America.
This guide or parts thereof may not be reproduced in any form without the written permission of the publishers.
Solutions for Crystal Growth
P A G E 2 - 2
cryst al growt h techni ques
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 184 Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
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There are several techniques for setting up crystallization experiments (often
termed trials), including sitting drop vapor diffusion, hanging drop vapor dif-
fusion, sandwich drop, batch, microbatch under oil, dialysis, and free interface
diffusion. Here we offer an overview of these crystallization techniques.
Sitting & Hanging Drop Crystallization
Sitting and hanging drop methodologies are very popular because they are easy
to perform, require a small amount of sample, and allow a large amount of flex-
ibility during screening and optimization.
Using the sitting drop technique (figure 1), one places a small (0.1 to 40 l)
droplet of the sample mixed with crystallization reagent on a platform in vapor
equilibration with the reagent. The initial reagent concentration in the droplet
is less than that in the reservoir. Over time the reservoir will pull water from the
droplet in a vapor phase such that an equilibrium will exist between the drop and
the reservoir. During this equilibration process, the sample is also concentrated,
increasing the relative supersaturation of the sample in the drop.
The advantages of the sitting drop technique include speed and simplicity. The
disadvantages are that crystals can sometimes adhere to the sitting drop surface
making removal difficult. This disadvantage can turn into an advantage where occa-
sionally the surface of the sitting drop can assist in nucleation. The sitting drop is an
excellent method for screening and optimization. During production, if sticking is a
problem, sitting drops can be performed in the Sandwich Box Setup.
Sitting drop crystallization may be performed using Micro-Bridges

or Glass
Sitting Drop Rods with VDX or Linbro

plates. Both plates can be sealed


with clear sealing tape or plain cover slides for easy viewing and access. Sitting
drop crystallization may also be preformed using the Cryschem Plate. The
Cryschem Plate is a specially designed plate with a post already in the center of
the reservoir. Finally, sitting drop crystallization can also be performed using
one of the 48 or 96 well plates made by Art Robbins Instruments (Intelli-Plate),
Corning (CrystalEX), Douglas Instruments (CrystalClear Strips), Greiner
(CrystalQuick), or Swissci (MRC).
Using the hanging drop technique (figure 2), one places a small (0.1 to 20 l)
droplet of the sample mixed with crystallization reagent on a siliconized glass
cover slide inverted over the reservoir in vapor equilibration with the reagent.
The initial reagent concentration in the droplet is less than that in the reservoir.
Over time the reservoir will pull water from the droplet in a vapor phase such
that an equilibrium will exist between the drop and the reservoir. During this
equilibration process, the sample is also concentrated, increasing the relative
supersaturation of the sample in the drop.
The advantages of the hanging drop technique include the ability to view the
drop through glass without the optical interference from plastic, flexibility,
reduced chance of crystals sticking to the hardware, and easy access to the drop.
The disadvantage is that a little extra time is required for setups.
Sandwich Drop Crystallization
The sandwich drop crystallization method is illustrated in figure 3. The sample
solution that is mixed with the precipitant is placed in the middle of a lower
siliconized glass cover slide. A siliconized glass cover slide is then set in posi-
tion along an upper edge. This allows for a small amount of space between the
cover slides but is close enough so the drop is sandwiched between them. This
technique offers an alternate equilibration method. However, the set up can be
tedious and the plates designed for this method typically have a larger footprint
than most.
Free Interface Diffusion
Free interface diffusion crystallization is used less frequently than sitting or
hanging drop vapor diffusion but it is one of the methods used by NASA in
microgravity crystallization experiments and at least one company has automated
and miniaturized the method. Using this method, one actually places the sample
in liquid contact with the precipitant. When doing so, one attempts to create a
clearly defined interface between the sample and the precipitant. Over time the
sample and precipitant diffuse into one another and crystallization may occur at
the interface, or on the side of high sample/low precipitant or low sample/high
precipitant. The technique allows one to screen a gradient of sample precipitant
concentration combinations. The technique can readily be performed in small
figure 1
Reservoir Solution
figure 3
P A G E 1 - 2
figure 2
H
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O H
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O
[ppt]
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=
[ppt]
reservoir
2
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capillaries (figure 4).
Batch
Batch crystallization is a method where the sample is mixed with the precipitant
and appropriate additives, creating a homogeneous crystallization medium
requiring no equilibration with a reservoir. The technique is popular with small
molecule crystallographers. The advantages to the technique are speed and
simplicity but the disadvantage is that only a narrow space of precipitant/sample
concentration can be sampled in a single experiment. A batch experiment can be
readily performed in a capillary, small container, or plate with a small reservoir.
One must be very close to the conditions which promote crystal growth in order
for this technique to be successful.
Microbatch Under Oil
In this technique, a small drop of the sample combined with the crystallization
reagent is pipetted under a layer of oil. For a true microbatch, the drop is placed
under paraffin oil (figure 5) which allows little to no evaporation, nor concentra-
tion in the drop. A modified microbatch can be performed when the drop is
placed under a mixture of paraffin oil and silicon oil, or straight silicon oil (figure
6). Such oils permit water vapor to permeate from the drop and allow sample
and reagent to concentrate. Unless the drop is equilibrated with a reservoir,
water will leave the drop until only solids remain.
The benefits of crystallization under oil include the use of very small sample and
reagent volumes with less concern for unwanted evaporation, the minimization
of surface interaction with the sample, the ability to precisely control sample and
reagent concentrations during the experiment, and the minimization of conden-
sation during temperature fluctuations.
Dialysis Crystallization
Dialysis crystallization involves placing the sample in a Dialysis Button which is
sealed with a dialysis membrane. Water and some precipitants are then allowed to
exchange while retaining the sample in the cell. The Dialysis Button is placed into
a suitable container holding the precipitant or crystallization media (figure 7). For
example, one might dialyze a sample requiring a high ionic strength for solubility
against a solution of low ionic strength. The technique allows for salting in and
salting out, as well as pH crystallization techniques.
Sample / Reagent Diffusion
Crystal
Sample Reagent
figure 4
Oil
Crystallization
Drop
Oil
Crystallization
Drop
figure 5
Oil
Crystallization
Drop
Oil
Crystallization
Drop
figure 6
Reservoir Solution
Dialysis Button
figure 7
P A G E 2 - 2
Solutions for Crystal Growth
1991-2009 Hampton Research Corp. All rights reserved. Printed in the United States of America.
This guide or parts thereof may not be reproduced in any form without the written permission of the publishers.
hangi ng drop vapor
di ffusi on cryst alli zati on
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 186 Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
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The hanging drop vapor diffusion technique is a very popular method for the
crystallization of macromolecules. The principle of vapor diffusion is straightfor-
ward. A drop composed of a mixture of sample and reagent is placed in vapor
equilibration with a liquid reservoir of reagent. Typically the drop contains a
lower reagent concentration than the reservoir. To achieve equilibrium, water
vapor leaves the drop and eventually ends up in the reservoir. As water leaves
the drop, the sample undergoes an increase in relative supersaturation. Both the
sample and reagent increase in concentration as water leaves the drop for the
reservoir. Equilibration is reached when the reagent concentration in the drop
is approximately the same as that in the reservoir.
Benefits of Hanging Drop Crystallization
Can be cost-effective.
Sample and reagents in contact with a siliconized glass surface.
Easy access to crystals.
Can perform multiple drops (experiments) with a single reservoir.
Good optics for viewing experiments.
Using the VDX Plate
The VDX Plate is a 24 well plate manufactured from clear polystyrene. It is
typically sealed with vacuum grease (HR3-510) and siliconized 22 mm circle
or square glass cover slides. The VDX Plate is also available with sealant which
is a convenient time saver. Rows of the plate are labeled A-D and columns are
labeled 1-6.
1. Apply a bead of vacuum grease along the top edge of the raised reservoir
A1 of the VDX Plate. It is recommended that one apply the vacuum grease
prior to pipetting the reagent. Create a circular bead on the upper edge of
the reservoir. Do not complete the circle. Leave a 2 mm opening between
the start and finish of the circular bead. Apply the cover slide, press to relieve
the air pressure and twist to close the gap. Or simply use the VDX Plate with
sealant. These plates come pregreased.
2. Pipet 1.0 ml of crystallization reagent into reservoir A1 of the VDX Plate.
(Note: Recommended reservoir volume is 0.5 to 1.0 ml)
3. Clean a siliconized 22 mm circle or square cover slide by wiping it with lens
paper and blowing it with clean, dry compressed air. Pipet 1 l of sample
into the center of a siliconized 22 mm circle or square cover slide. (Note:
Recommended total drop volume is 0.1 to 20 l)
4. Pipet 1 l of reagent from reservoir A1 into the drop on the cover slide con-
taining the sample. (Note: Some prefer to mix the drop while others do
not. Proponents of mixing leave the pipet tip in the drop while gently
aspirating and dispensing the drop with the pipet. Mixing ensures a
homogeneous drop and consistency drop to drop. Proponents of not mix-
ing the drop simply pipet the reagent into the sample with no further
mixing.)
5. Holding the cover slide with forceps, the PEN-VAC

, Cover Slide Vacuum


Gadget, or on the edge between your thumb and forefinger, carefully yet
without delay, invert the cover slide so the drop is hanging from the cover
slide.
6. Position the cover slide onto the bead of grease on reservoir A1. Gently press
the slide down onto the grease and twist the slide 45 to ensure a complete
seal.
7. Repeat for reservoirs 2 through 24.
VDX Plate Tips
Note the VDX Plate has a raised cover to protect the cover slides during
transport and storage.
To access a drop and/or reservoir, simply grasp the edge of the cover slide
with forceps or fingertips, twist and pull gently.
VDX Plates can be stacked for convenient storage.
One can pipet multiple drops onto the cover slide. This technique is often
useful when screening additives since one can use the same reservoir with
multiple drops with each drop containing a different additive. This technique
can also be used to screen different drop sizes and ratios versus the same
reservoir. Use care to avoid mixing the drops during pipetting, plate transport,
and plate viewing.
The 0.96 mm Thick glass cover slides or the plastic cover slides are very
durable and most tolerant to rough handling.
Plates for Hanging Drop Crystallization
VDX
VDX with sealant
VDXm
VDXm with sealant
VDX48 with sealant
Modular VDX
Linbro

Greiner ComboPlate
figure 2 Crystallization Droplet
(2 l Sample / 2 l Reagent)
Siliconized cover slide
Solutions for Crystal Growth
1991-2009 Hampton Research Corp. All rights reserved. Printed in the United States of America.
This guide or parts thereof may not be reproduced in any form without the written permission of the publishers.
figure 1
Process of Vapor Diffusion
187
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Solutions for Crystal Growth
si tti ng drop vapor
di ffusi on crystalli zati on
P A G E 1 - 3
The sitting drop vapor diffusion technique is a popular method for the crystal-
lization of macromolecules. The principle of vapor diffusion is straightforward.
A drop composed of a mixture of sample and reagent is placed in vapor equili-
bration with a liquid reservoir of reagent. Typically the drop contains a lower
reagent concentration than the reservoir. To achieve equilibrium, water vapor
leaves the drop and eventually ends up in the reservoir. As water leaves the drop,
the sample undergoes an increase in relative supersaturation. Both the sample
and reagent increase in concentration as water leaves the drop for the reservoir.
Equilibration is reached when the reagent concentration in the drop is approxi-
mately the same as that in the reservoir.
Benefits of Sitting Drop Crystallization
Can be cost-effective.
Can be time-efficient.
Often easier when using detergents, organics and hydrophobic reagents.
Drops can be positioned in a stable sitting position.
Compatible with gels.
Using the Cryschem

Plate
The Cryschem Plate is a 24 well plate manufactured from clear polystyrene. Each
well contains a post in the center which is elevated above the bottom of the res-
ervoir. The smooth, concave depression in the post can hold up to 40 l drops
and the reservoir can hold up to 1.2 ml of reagent. The Cryschem Plate is sealed
with either clear sealing tape or film, or plain 22 mm circle or square glass cover
slides. Rows are labeled A-D and columns are labeled 1-6.
1. Pipet 0.7 ml of crystallization reagent into reservoir A1 of the Cryschem Plate.
(Note: Recommended reservoir volume is 0.5 to 1.0 ml)
2. Pipet 1 l of sample into the post of reservoir A1. (Note: Recommended total
drop volume is 0.1 to 40 l)
3. Pipet 1 l of reagent from reservoir A1 into the drop in post A1. (Note: Some
prefer to mix the drop while others do not. Proponents of mixing leave
the pipet tip in the drop while gently aspirating and dispensing the drop
with the pipet. Mixing ensures a homogenous drop and consistency drop
to drop. Proponents of not mixing the drop simply pipet the reagent into
the sample with no further mixing.)
4. Repeat steps 1 through 3 for the remaining 23 reservoirs.
5. Seal the Cryschem Plate with clear sealing tape or film.
Cryschem Plate Tips
Use Crystal Clear Sealing Tape to seal two rows at a time.
To access a drop and/or reservoir of a Cryschem Plate sealed with tape, simply
make a circular incision in the tape using the inside of the reservoir as a guide.
Use a sharp blade to cut the tape and hold the incised piece of tape with
forceps. The opening can be sealed with another strip of tape or a plain 22
mm circle or square glass cover slide and vacuum grease.
Using Micro-Bridges

The Micro-Bridge is a small bridge (inverted U) manufactured from clear polysty-


rene or clarified polypropylene which contains a smooth, concave depression in
the center of the top region of the bridge (figure 3). The Micro-Bridge can hold
up to 40 l drops. It is inserted into the reservoirs of VDX or Linbro

plates
to perform a sitting drop vapor diffusion experiment. The design is such that the
bridge is quite stable in the reservoir and does
not require the Micro-Bridge to be bonded to
the plate. The Micro-Bridge can be removed
from the plate for crystal manipulation and
observation if desired.
1. Pipet 1.0 ml of crystallization reagent
into reservoir A1 of a VDX plate. (Note:
Recommended reservoir volume is 0.5 to
1.0 ml)
2. Place a clean (blow the Micro-Bridge with clean, dry compressed air before
use) Micro-Bridge into the bottom of reservoir A1 such that the concave
depression in the Micro-Bridge is facing up.
3. Pipet 1 l of sample into the Micro-Bridge in reservoir A1. (Note:
Recommended total drop volume is 0.1 to 40 l)
4. Pipet 1 l of reagent from reservoir A1 into the drop in the Micro-Bridge A1.
(Note: Some prefer to mix the drop while others do not. Proponents of
mixing leave the pipet tip in the drop while gently aspirating and dis-
pensing the drop with the pipet. Mixing ensures a homogenous drop and
consistency drop to drop. Proponents of not mixing the drop simply pipet
the reagent into the sample with no further mixing.)
5. Repeat steps 1 through 3 for the remaining 23 reservoirs.
6. Seal the plate with clear sealing tape or sealant and plain glass cover slides.
Micro-Bridge Tips
To access a drop and/or reservoir sealed with tape, simply make a circular inci-
sion in the tape using the inside of the reservoir as a guide. Use a sharp blade
to cut the tape and hold the incised piece of tape with forceps. The opening
can be sealed with another strip of tape or a plain 22 mm circle or square glass
cover slide and vacuum grease.
Micro-Bridges can be removed for crystal seeding, mounting, manipulation,
and observation.
Micro-Bridges are designed as disposable devices. It is not recommended to
wash and re-use Micro-Bridges.
Micro-Bridges cannot be siliconized or autoclaved.
Crystallization Droplet
(2 l Sample / 2 l Reagent)
figure 2
figure 3
H2O H2O
[ppt]
drop
=
[ppt]
reservoir
2
figure 1
Process of Vapor Diffusion
188
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Using Glass Sitting Drop Rods

The Glass Sitting Drop Rod is a small, solid rod manufactured from clear glass
that has a smooth, concave depression in the center of the top region (figure
4). The opposite end of the glass rod is flat and it can hold up to a 100 l drop.
It is inserted into the reservoirs of VDX plates to perform a sitting drop vapor
diffusion experiment. The Glass Sitting Drop Rod can be secured to the bottom
of the plate using vacuum grease or can be left unattached to the bottom of the
plate. It can be removed from the plate for crystal manipulation and observation
if desired.
1. Pipet 1.0 ml of crystallization reagent into reservoir A1 of a VDX plate. (Note:
Recommended reservoir volume is 0.5 to 1.0 ml; Additional Note: If
vacuum grease will be used to secure
the Glass Sitting Drop Rod to the
plate, apply the grease to the rod
and insert the rod prior to pipetting
reagent into the reservoir.)
2. Place a clean (blow the Glass Sitting
Drop Rod with clean, dry compressed air
before use), siliconized glass rod into the
bottom of reservoir A1 such that the concave depression in the Glass Sitting
Drop Rod is facing up.
3. Pipet 1 l of sample into the Glass Sitting Drop Rod in reservoir A1.
4. Pipet 1 l of reagent from reservoir A1 into the drop in the glass rod A1.
(Note: Some prefer to mix the drop while others do not. Proponents of
mixing leave the pipet tip in the drop while gently aspirating and dis-
pensing the drop with the pipet. Mixing ensures a homogenous drop and
consistency drop to drop. Proponents of not mixing the drop simply pipet
the reagent into the sample with no further mixing.)
5. Repeat steps 1 through 3 for the remaining 23 reservoirs.
6. Seal the plate with two strips of clear sealing tape or a plain glass cover slide
and sealant.
Glass Sitting Drop Rods Tips
To access a drop and/or reservoir of a plate sealed with tape, simply make a
circular incision in the tape using the inside of the reservoir as a guide. Use
a sharp blade to cut the tape and hold the incised piece of tape with forceps.
The opening can be sealed with another strip of tape or a plain 22 mm circle
or square glass cover slide and vacuum grease.
Glass Sitting Drop Rods can be removed for crystal seeding, mounting,
manipulation, and observation.
Siliconize Glass Sitting Drop Rods before use.
Glass Sitting Drop Rods may be washed and used over and over again.
However, if vacuum grease is used to secure the Glass Sitting Drop Rod to the
bottom of the reservoir, we wish you good luck in completely removing the
grease from the rod.
Glass Sitting Drop Rods may be autoclaved.
CrystalClear Strips

CrystalClear Strips consist of a plastic frame and 12 polystyrene strips with a


total experiment capacity of 96 per plate. Each strip contains 8 reservoirs and
platforms. Typical reservoir volumes are 100 l. The smooth, concave depression
on the platform above the reservoir can hold up to a 10 l drop. The CrystalClear
Strip is sealed with clear sealing tape or film.
Pipet 100 l of crystallization reagent in each of the 96 reservoirs. Pipet 1 l of
sample into the depression on the ledge of the first reservoir A1. Pipet 1 l of
reagent from reservoir A1 into the drop on the ledge above reservoir A1. Repeat
steps 2 and 3 for the remaining 95 reservoirs and wells. Seal the CrystalClear
Strips with clear sealing tape or film.
CrystalClear Strips Tips
The strips are available with and without a concave depression for drop place-
ment.
While pipetting reagents into the reservoirs, place a clean pipet tip into the
first empty reservoir. Move the clean tip ahead one empty reservoir with each
reagent addition. This will help one keep track of their pipetting position in
the plate.
Sandwich Box

The Sandwich Box consists of a square polystyrene box, a plastic support, and
a siliconized 9 well glass plate. The Sandwich Box is used when a common
dehydrant system is desired as well as very large drops. Enormous drops can
be pipetted into the siliconized glass wells. The siliconized glass plates offer
excellent optics and can be removed from the plastic box to inspect the drop
for birefringence without optical interference from plastic. Sandwich Boxes
offer unique vapor equilibration kinetics and are very easy to access for crystal
seeding, manipulation, and mounting. The plates are often used for heavy atom
screening and derivatization and are useful for long-term crystal storage when
each well is sealed with a glass slide and vacuum grease.
Open the Sandwich Box and place a plastic support, bottom side facing up into
the box. Apply a bead of vacuum grease to the outer top edge of the box or the
outer lower edge of the lid. Pour 25 ml of crystallization reagent or common
dehydrant into the Sandwich Box. Place the siliconized 9 well glass plate on
top of the inverted plastic support. Pipet the sample into one of the nine wells.
Add the appropriate crystallization reagent to each drop. Place the cover on the
Sandwich Box.
Sandwich Box Tips
Apply a thin bead of vacuum grease around a single depression of the glass
plate and seal the depression with a plain glass cover slide for long term crystal
storage.
Use a siliconized glass depression plate to test a small amount of sample for
solubility with various crystallization reagents.
P A G E 2 - 3
figure 4
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
si tt i ng drop vapor
di ffusi on cryst alli zati on
189
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P A G E 3 - 3
Solutions for Crystal Growth
96 Well Plates
The 96 well sitting drop plates offer a variety of drop well configurations and
flexibility in a standard microplate footprint. The 8 x 12 reagent wells in 9 mm
offset can be filled with automated liquid handling systems or manual, single, and
multichannel pipets with a typical reagent fill volume of up to 100 l. The diversity
of the various sample drop wells allow for automated and manual pipetting into
a variety of well shapes, volumes and geometries. Materials range from optically
clear polystyrene to low birefringent polymers. The plates can be manually or
automatically sealed with optically clear sealing tape or film.
Plates for Sitting Drop Crystallization
Cryschem
VDX with Micro-Bridges

or Glass Sitting Drop Rods


Linbro

with Micro-Bridges or Glass Sitting Drop Rods


Greiner ComboPlate with CrystalBridge
Douglas Instruments CrystalClear Strips
Sandwich Box
Intelli-Plate
Corning CrystalEX
Greiner CrystalQuick
Douglas Instruments Vapor Batch
Swissci MRC
1991-2009 Hampton Research Corp. All rights reserved. Printed in the United States of America.
This guide or parts thereof may not be reproduced in any form without the written permission of the publishers.
cryst alli zat i on under oi l
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 190 Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
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1991-2009 Hampton Research Corp. All rights reserved. Printed in the United States of America.
This guide or parts thereof may not be reproduced in any form without the written permission of the publishers.
Method
Crystallization under oil is a method where a small drop of sample combined
with the crystallization reagent of choice is pipetted under a layer of oil. This is
also known as microbatch crystallization.
Oils can also be used as a barrier between the reservoir and the drop in tradi-
tional hanging or sitting drop crystallization experiments. This is known as vapor
diffusion rate control.
Description of Microbatch
The crystallization of proteins
under a thin layer of paraffin
oil was originally described by
Chayen et al. (Appl. Cryst. 23
(1990) 297). In this technique, a
small drop of sample combined
with the reagent of choice is
pipetted under a small layer of
paraffin oil (figure 1). The oil generally used is a mineral oil of branched paraffins
in the C20+ range and allows for little to no diffusion of water through the oil.
Essentially all of the reagents in a batch or microbatch experiment are present
at a specific concentration. Furthermore, there is no significant concentration of
either the protein or the reagent within the crystallization drop.
Description of Modified Microbatch
DArcy et al. (A novel approach to crystallizing proteins under oil, Journal
of Crystal Growth 168 (1996) 175-180) modified the microbatch under oil
technique by using silicone fluids
which are polymeric compounds
composed of repeating dimeth-
ylsiloxane units -(Si(CH
3
)
2
-O-)
n
-.
Using a mixture of 1:1 silicon oil
and paraffin oil, also known as Als
Oil, one can perform a microbatch
experiment under oil and have
diffusion of water from the drop
through the oil, hence a micro-
batch experiment that does allow
for concentration of the sample
and the reagents in the drop (figure 2).
Performing Microbatch / Modified Microbatch
Pipet 6 ml of 100% paraffin oil or 6 ml of 1:1 paraffin/silicon oil (Als Oil) into a
72 Well Microbatch Plate, as shown in figure 1 or 2. Note: One can also utilize
other ratios of paraffin oil and silicon oil to vary the rate of diffusion from
the drop (higher % of silicon oil = more rapid diffusion and evaporation).
Pipet the sample into the appropriate cone-shaped depression in the microbatch
plate followed by addition of the reagent. Typical drop ratios and final drop sizes
are 1:1 and 1 to 2 l. Drops up to 10 l can be achieved under oil using the
microbatch plate. Place plate cover over microbatch plate to prevent dust and
debris from entering experiment. The microbatch method can also be performed
in round bottom, clear 96 well plates. The oil, reagent, and sample can be mixed
quickly and efficiently for fast throughput by using an 8 or 12 channel pipetter.
Description of Vapor Diffusion Rate Control
Chayen (A novel technique to control the rate of vapour diffusion, giving larger
protein crystals J. Appl. Cryst 30 (1997) 198-202) has described a technique
where oils can be used to vary the rate of vapor diffusion. Using mixtures of
paraffin and silicon oil, Chayen reported fewer, larger crystals in the drop.
Using a standard hanging or sitting drop vapor diffusion setup, the drop is first
mixed with reservoir solution, thus preventing oil from entering the drop. Then,
200 l of oil is applied over the reservoir solution (figure 3). The oil acts as a
barrier to vapor diffusion between the reservoir and the drop. Using 100% paraf-
fin oil allows limited amount of vapor diffusion to occur, which in turn causes
the drop to behave like a batch experiment. The drop will then eventually dry
up due to evaporation through the polystyrene plate. Using 100% silicon oil will
give results similar to that when no oil is used. When using a mixture of the
two oils, the rate of vapor diffusion between the drop and the reservoir may
be controlled. The rate of vapor diffusion is also a function of thickness of the
oil layer over the reservoir. Chayen evaluated oil volumes between 100 and
700 l. Oil volumes of 50 to 100 l resulted in crystals similar to the control
without oil. Oil volumes greater than 700 l have a significant delay in the
onset of crystallization, with improved crystal size. Results using hanging drop
were more pronounced than sitting drop which may be due to either surface
effects or the drop geometry in relation to the reservoir which could influence
vapor diffusion kinetics.
Performing Vapor Diffusion Rate Control
Prepare a VDX or Cryschem Plate for a sitting or hanging drop vapor diffusion
experiment. After the reservoir has been added to the drop, pipet between 200
and 700 l of a mixture of paraffin/silicon oil onto the reservoir (figure 3). Seal
the plate.
Plates for Microbatch Crystallization
Douglas Instruments Vapor Batch
Greiner 72 Well Microbatch
Greiner Imp@ct
Swissci MRC Under Oil 96 Well Crystallization Plate
figure 1
figure 2
Paraffin
Oil
Als
Oil
figure 3
Oil Layer
Reservoir
Solution
Solutions for Crystal Growth
191
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Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
di alysi s crystalli zati on
Solutions for Crystal Growth
Method
Crystallization by dialysis is an easy variation to the typical vapor diffusion
method used to grow crystals. In the dialysis method, the sample in question is
separated from the precipitant by a semi-permeable membrane which allows
small molecules such as ions, additives, buffers, and salts to pass but prevents
biological macromolecules from crossing the membrane. Equilibration kinetics
depend upon the molecular weight cut-off of the dialysis membrane, the precipi-
tant, the ratio of the volume, the concentration of the components inside and
outside of the dialysis cell, and the geometry of the cell.
Description
The Dialysis Buttons offered by Hampton Research are machined from
transparent perspex. The button has a chamber which varies from 5 to 350 l
depending upon which size button
one chooses to use. The sample
is placed in this chamber so as to
create a slight dome of liquid at the
top edge of the button. A dialysis
membrane (having the appropriate
molecular weight cut-off) is placed
over the top of the button/sample
and is held in place with an O-ring.
The O-ring is held in place by a groove in the button. Dialysis Buttons are notori-
ously tricky to set up since beginners often trap air bubbles between the sample
solution and the membrane which impedes dialysis. With a little practice using
a golf tee or applicator, one can master the technique. Dialysis Buttons are
supplied with O-rings and a golf tee. Dialysis membrane discs and applicators
are available separately. The applicator is used to apply the membrane and the
O-ring to the buttons.
Using the Dialysis Button
A typical dialysis experiment is used to take the sample from the presence of
a high ionic strength solution to a lower ionic strength solution. However, the
technique can just as easily be used to proceed from low ionic strength to a
higher ionic strength. This is accomplished by placing the sample in high ionic
strength into the Dialysis Button, sealing the button with a dialysis membrane,
and placing the sealed button in a solution of ionic strength lower than that
inside the button. Salts, ligands, and compounds smaller than the pore size
of the dialysis membrane will leave the button as long as their concentration
is lower on the opposite side of the membrane. Once the concentration of the
diffusible species is the same on both sides of the membrane, the system is in
equilibrium.
Cleaning
The buttons can be cleaned with soap and deionized water. Do not clean them
with organic solvents as this may turn the perspex opaque.
Practical Example
The following two practicals offer examples of how to set up a dialysis experi-
ment.
Practical 1 - Carboxypeptidase A
1. Using Carboxypeptidase A (Sigma-Aldrich CO386
or CO261), make an 8 to 20 mg/ml solution of the
Carboxypeptidase A in 20 mM Tris HCl pH 7.5, 1.5
M LiCl.
2. Place 100 ul of 10 mg/ml carboxypeptidase in 20
mM Tris HCl, 1.5 M LiCl, pH 7.5 in a 100 ul Dialysis
Button. The droplet should have a slight dome
shape following the hemispheric edge of the top
of the button.
3. Seal the button with dialysis membrane. Using a
one inch (2.5 cm) square of dialysis membrane
which has equilibrated in water, place the mem-
brane over the top of the button. Place an inverted
golf tee on top of the membrane and button. Roll
the O-ring down the applicator until the O-ring
rolls off onto the edge of the button. Roll the O-ring into the machined groove
on the edge of the button. Remove the applicator. There should be no bub-
bles between the membrane and the sample inside the button. Bubbles will
prevent dialysis. Some researchers prefer to use the rubber tipped plunger of
a syringe with a modified syringe body to apply the O-ring. Others prefer to
use an applicator. Try each and see which method works best.
4. Place 0.9 ml of 20 mM Tris HCl, pH 7.5 in the reservoir of a VDX Plate, a
Linbro

Plate, or small chamber which can be sealed.


5. Place the Dialysis Button in the well, membrane side up. Be sure the reservoir
solution covers the top of the membrane/button. Seal the VDX plate using
grease and a cover slide.
6. Observe under a microscope. Crystals will appear within 2 to 3 days. Final
concentration of LiCl will be 0.15 M.
Reference for the above protocol: Dr. Jim Pflugrath and Dr. Gary Gilliland, Cold Spring Harbor Laboratory
Protein Crystallography Workshop.
O-Ring
Golf Tee
Dialysis Button
Sample Chamber
O-Ring Support
figure 1
figure 2a
Cover Slide (or Sealing Tape)
Well of VDX
Crystallization Plate
Reservoir Solution
Dialysis Button
Vacuum
Grease
figure 3
P A G E 1 - 2
figure 2b
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 192 Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
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1991-2009 Hampton Research Corp. All rights reserved. Printed in the United States of America.
This guide or parts thereof may not be reproduced in any form without the written permission of the publishers.
Practical 2 - Lysozyme
1. Prepare 10 mg/ml lysozyme in 50 mM Sodium acetate trihydrate pH 4.5. Filter
the solution using a 0.2 micron filter.
2. Fill a 100 l Dialysis Button with 100 l of the lysozyme solution as described
for the carboxypeptidase practical.
3. Pipet 1 ml of 50 mM Sodium acetate trihydrate buffer into a small (5 ml) bea-
ker.
4. Place the filled button, membrane side up in the beaker.
5. Pipet a small amount of concentrated Sodium chloride into the beaker such
that the final concentration of Sodium chloride in the beaker is 0.2 M. Seal the
beaker with parafilm and store at room temperature.
6. Increase the concentration of Sodium chloride each day by 0.2 M. Repeat until
crystals are observed in the button.
Reference for the above protocol: Crystallization of nucleic acids and proteins, a practical approach. Edited by A.
Ducruix and R. Giege, Oxford University Press, 1992.
Considerations
Just as in a vapor diffusion experiment, the path is often as important as the
endpoint in a dialysis experiment. The path is the equilibration course which the
solution inside and outside the button take toward achieving equilibrium. This
course can be changed by manipulating the following:
Ratio of button volume/reservoir volume
Button and reservoir components & concentration
Molecular weight cutoff of dialysis membrane
Viscosity of solutions
Plus the usual assortment of crystallization variables
including pH, sample concentration, temperature, etc.
Variations of Dialysis
Macrodialysis
The sample is loaded into dialysis tubing of the appropriate molecular weight
cutoff and is dialyzed against the appropriate reservoir solution. This method
typically requires at least 100 l of sample and can be performed with liters of
sample in large dialysis tubing.
Zeppenzauer Cells
Capillary tubes are closed with dialysis tubing or gel plugs. See Zeppenzauer, M.
1971, Methods In Enzymology, 22, 253.
Microcap Dialysis
The sample is placed in a glass capillary with one end sealed with wax, the other
with dialysis membrane. The tube is placed in a microcap/small centrifuge tube
filled with the appropriate reagent. See Crystallization of nucleic acids and pro-
teins, a practical approach, Edited by A. Ducruix and R. Giege, Oxford University
Press, 1992.
Double Dialysis
This method reduces the rate of equilibration and can provide enhanced control
over the crystallization of the sample. Simply put, a Dialysis Button is prepared
and placed inside a reservoir sealed with a dialysis membrane, which is in turn
placed inside another reservoir. Confused? See Thomas, D.H., et al., 1989,
Protein Engineering, 2, 489.
References and Readings
1. Crystallization of nucleic acids and proteins, Edited by A. Ducruix and R. Giege, The Practical Approach Series, Oxford
Univ. Press, 1992.
2. Preparation and analysis of protein crystals. McPherson, A. Eur. J. Biochem. 189, 1-23, 1990.
3. Zeppenzauer, M. et al., Crystal. of horse liver alcohol dehydrogenase complexes from alcohol solutions. Acta Chem Scan,
21, 1099, 1967.
4. Christopher Bunick, A.C.T. North, and Gerald Stubbs., Evaporative microdialysis: an effective improvement in an estab-
lished method of protein crystallization. Acta Cryst. D56, 1430-1431, 2000.
Solutions for Crystal Growth
P A G E 2 - 2
di alysi s cryst alli zati on
Single Crystal
Clear Drop
Skin/Precipitate
Precipitate
Precipitate/Phase
Quasi Crystals
Microcrystals
Needle Cluster
Plates
Rod Cluster
figure 1
Typical observations in a crystallization experiment.
193
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Solutions for Crystal Growth
Observing the Experiment
Gently set the plate onto the observation platform. If the
platform is smooth and free of protrusions, one may simply
slide the plate in the X and Y directions on top of the viewing
platform to view each of the drops. Use low magnification to
view and center the drop in the field of view. Scan the drops
at 20 to 40x magnification. When something suspicious
appears, increase the magnification to 80 or 100x for a better
view. Scan the entire depth of the drop using the fine focus
control of the microscope. Sometimes crystals will form at
different depths of the drop because different areas of the
drop can equilibrate at different rates. Also, crystals some-
times form at the top of a drop and as the crystal gains mass,
it will fall to a lower portion of the drop. Scrutinize every-
thing until you are familiar with the differences between
crystals, microcrystals, precipitate, and sweater fuzz. True
crystals will feature edges. Precipitate does not have edges.
Crystals can appear as needles, blades, walnuts, spherulites,
plates, and various geometric shapes. Crystals vary in size
anywhere from a barely observable 20 microns to 1 or more
mm but most seem to fall in the range of 0.05 to 0.5 mm.
Figure 1 shows typical examples of what one might observe
in a crystallization experiment.
Diffractable Crystal
Crystals useful for x-ray diffraction analysis are typically
single, 0.05 mm or larger, and free of cracks and defects.
Differentiating Between Microcrystals & Precipitate
Microcrystals (less than 0.02 mm) can be difficult to dif-
ferentiate from precipitate, especially under low power or
with a low to medium quality microscope. Differentiate
microcrystals from amorphous precipitate by looking for
birefringence (light colored shiny spots under a polarizer in
dark field mode = crystals). Other tests to differentiate crys-
tals from precipitate include streak seeding, or the use of a
small amount (1 l) of colored, low molecular weight, water
soluble dye (crystal violet and methylene blue will often pen-
etrate the solvent channels of macromolecular crystals and
color them, where as precipitate will not be colored).
Precipitate can appear as clumps, fine wispy clouds, or
anything in between and can range in color from white
to yellow, brown, or rust. During screening and very pre-
liminary optimization, one may wish to observe the drops
immediately after setup, one day later, and each day there-
after for the first week. Observations may be performed
once a week thereafter until the drops turn into a crust of
deceased sample and reagent. Never throw plates away until
the drop is dead. Why? Most crystallization plates are made
from polystyrene which allows for some evaporation over
time. Evaporation leads to increased relative supersaturation
and over time, may also lead to an increase in crystals. Time
also can lead to changes in the protein (denaturation of less
stable forms, proteolytic cleavage, and other changes) which
might promote crystallization. Take careful notes during
observations and be especially conscious of changes that
occur between observations. Most crystallization observa-
tions are done at room temperature since this is where one
will find most microscopes and it is most comfortable.
Cold Experiments
4C experiments may be observed by moving the micro-
scope into a cold room. Allow time for the microscope
to equilibrate to 4C to prevent fogging of the optics as
well as unnecessary temperature transfer from the warm
microscope to the cold experiment. Wear a warm jacket
with gloves to stay as comfortable as possible in the cold
room. Excessive moisture in a cold room can be very
destructive to a microscope so check with your mainte-
nance group to keep the cold room as dry as possible. If a
cold room is unavailable, one is forced simply to work fast,
moving plates from an incubator to the microscope care-
fully and making rapid, yet thorough observations. Move
only one plate at a time and gently close the incubator
door between transfers since slamming the door will cause
vibrations which can influence crystallization. Cold experi-
ments tend to fog up rapidly, especially if the light source
is hot (if no infrared filters or light pipes are used). This
is difficult to avoid and is one reason researchers prefer
working in cold rooms.
1991-2009 Hampton Research Corp. All rights reserved. Printed in the United States of America.
This guide or parts thereof may not be reproduced in any form without the written permission of the publishers.
The technical mumbo jumbo first. The physical properties of isotropic materials,
such as glasses, liquids, and amorphous materials do not depend on direction.
However, most properties of a wide variety of crystals (including liquid crystals)
do show such variation. This anisotropy of physical properties originates in the
anisotropic build-up of the materials (crystal structure). Anisotropy in the optical
properties of uniaxial crystals is referred to as either birefringence or dichroism,
depending on whether the index of refraction or the absorption coefficient is con-
cerned. Birefringence means that there are two distinct speeds with which light
can propagate, depending on the direction of propagation. When a light ray splits
into two beams as it passes through a material, the effect is called birefringence
(or double refraction) and the material is birefringent. If you look at something
through a birefringent material, youll see double. The word birefringence comes
from the Latin bi- (twice) plus refringere (to break up). Thus, the light rays are
broken in two by a birefringent material. One well-known example of a birefrin-
gent medium is crystalline calcite (calcium carbonate). If you look at the world
through a clear crystal of calcite (calcium carbonate), you will see double. Place
such a crystal on a drawing, and you'll see two overlapping copies of the drawing.
The molecular structure of calcite causes double refraction, in which each light ray
is split into two rays that emerge from the crystal at slightly different angles. Calcite
shows this more clearly than most crystals, but quartz and many other crystalline
minerals also split light ray.
Now, the practical interpretation for crystal growers. You might hear the word
birefringence used quite often by crystal growers when viewing crystals under a
microscope. Here, crystal growers are stretching the definition of the term bire-
fringence to describe the colorful display produced by biological macromolecular
crystals when polarized light is passed through the crystal. See figure 1.
A light microscope with polarizing optics is required to observe birefringence. The
following path is a typical setup where light passes from the light source through
the first polarizing lens, next the specimen (crystal), then the second polarizing
optic, finally the magnifying optics and into your eye. On many typical polarization
setups, the second polarizing filter can be rotated while the specimen is stationary.
Rotating the polarizing optic without something to rotate the plane of polarized
light in the path (i.e. a crystal) will result in one seeing light, dark, light, dark, as
the filter is rotated. But if a crystal with birefringent properties (i.e. a biological
macromolecular crystal) is positioned in between the two polarizing filters, one
will observe changing colors as the polarizing filter is rotated. Specifically, when
the polarizing filters are aligned such that the field is dark, a birefringent object
(crystal) will glow with color.
Birefringence is one way we can differentiate amorphous precipitate from
microcrystals in a drop when viewed under a microscope. Precipitate does not
have birefringent properties while most biological macromolecular crystals do.
One drawback with using birefringence in today's crystal growth world is that most of
the crystallization devices utilized are made from plastic such as polystyrene and poly-
propylene. These plastics are optically active and can be birefringent. In fact, often times
the colors we see displayed in crystals are contributions from the birefringent plastic.
See figure 2. However, it is still possible to observe microcrystalline birefringence in the
plastic trays, but there is usually a contributory effect from the plates used to grow the
crystals. One way to avoid this is to grow crystals in a glass device or at least observe the
crystals in a path that is free of birefringent plastics. Some crystallization plates are avail-
able in a low birefringent material.
Birefringent precipitates will glow, sparkle, or glisten. See figure 3.
To test for birefringence, position the polarizers so the field of view is dark
WITHOUT the crystallization plate or setup. Place the tray into position on the
microscope. If a crystal is birefringent, some of the light passing through the crys-
tal will be rotated and pass through the second (analyzing) polarizing filter. The
intensity of the transmitted light will increase and decrease as the crystal or the
polarizer is rotated. Remember, birefringence is NOT ALWAYS clearly visible when
polystyrene or polypropylene is in the light path (i.e. when you use plastic slides or
crystallization plates). However, a birefringent crystal viewed in most plastic trays
or plastic cover slides will have a different color than the background (i.e. plastic
plate) or foreground (plastic slide). Finally, birefringence is a property of crystals,
both biological (proteins, peptides, and nucleic acids) and inorganic crystals (salts).
Birefringence is MORE pronounced in inorganic crystals (salt).
A quick comment on what to do with birefringent precipitates. Streak seeding is
a common and often successful method of taking advantage of microcrystalline
precipitate to grow large single crystals.
figure 2
figure 1
figure 3
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1991-2009 Hampton Research Corp. All rights reserved. Printed in the United States of America.
This guide or parts thereof may not be reproduced in any form without the written permission of the publishers.
Solutions for Crystal Growth
bi refri ngence
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Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
Solutions for Crystal Growth
P A G E 1 - 2
First of all, before deciding on how to distinguish whether the crystal is inorganic
(salt) or biological (protein), take a picture of the crystal before you destroy it
or make it disappear.
The most definitive test is to obtain an x-ray diffraction pattern of the crystal. A
diffraction pattern of a protein crystal may look like that in figure 1. Want to take
a less direct approach? Read on.
The following are ways to differentiate a biological crystal from an inorganic crystal.
1. Dehydration
A biological crystal typically has very significant solvent content and will dehydrate
when removed from the drop or when the drop is allowed to evaporate from
around the crystal (figure 2). Inorganic crystals typically do not possess large
solvent channels and have very little solvent content. Removing an inorganic
crystal from the drop or allowing the drop to evaporate from around the crystal
will typically not destroy or change the appearance of the inorganic crystal.
2. Physical Manipulation
A biological crystal behaves more like an ordered gel than a hard crystal and will
powder, crumble, or break easily when touched with a probe such as a Micro-
Tool or needle (figure 3). Inorganic crystals can also break apart, but they require
more force and typically make a click or crunching sound when breaking apart
under the force of a probe. The inorganic crystals are typically more dense than
protein crystals and once broken, the pieces fall quickly and stay put on the
bottom of the drop.
3. Birefringence
A biological crystal, unless it is cubic, will be weakly birefringent under cross
polarizers (figure 4). Inorganic crystals are typically strongly birefringent under
cross polarizers. Some plastic plates and materials are also birefringent so this
test is more easily performed and interpreted in an all-glass environment or in a
plate made from a low birefringent plastic.
4. Dye absorption
A biological crystal typically has large solvent channels which will accommodate
a small molecule dye. Small molecule dyes can travel into these solvent channels
and color the crystal (figure 5). Inorganic crystals do not possess such solvent
channels and will not absorb the small molecule dye. Dyes are chemicals and
have solubility limits. So it is possible that a crystal grown in a reagent of high
relative supersaturation may have a reagent concentration that will precipitate
or even crystallize the dye. Most dyes under such conditions will crystallize into
needles or whiskers and of course be colored. So before adding dye, take a
picture or memorize the location of crystals in the drop in case the crystal itself
forms crystals. Finally, diluting the drop with dye can sometimes decrease the
relative supersaturation in the drop to the point where the biological crystal will
dissolve. Once the drop equilibrates again with the reservoir, the crystal may
reappear and it may appear in a location different from the original crystal. For
dyes, consider Izit (HR4-710) from Hampton Research.
5. Control Experiment
Using the same sample buffer, same reagent, same volumes and same hardware,
set an experiment identical to the one that produced the crystals, except leave
the sample out of the experiment. Simply replace the sample with the sample
buffer. Dont get lazy and use water instead of sample buffer because the sample
buffer may be a variable in the formation of the crystals. If a crystal forms that
appears visually similar to the original crystal you likely have an inorganic crystal
in the original setup.
6. Run a Gel
Collect, wash, and dissolve the crystals. Run the sample on SDS-PAGE. If a band
indicates the presence of your sample, there is a high probability that your origi-
nal crystals are biological.
Hampton Research would like to thank Joe Luft at the Hauptman Woodward
Medical Research Institute for his help in putting together the information about
inorganic and biological crystals.
figure 1
X-ray diffraction pattern from a protein crystal.
figure 2
Below is a happily hydrated protein crystal. On the next page is an unhappy,
dehydrated protein crystal, removed from the drop.
salt or bi ologi cal crystals?
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 196 Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
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1991-2009 Hampton Research Corp. All rights reserved. Printed in the United States of America.
This guide or parts thereof may not be reproduced in any form without the written permission of the publishers.
Dehydrated
Protein Crystal
Before: Pre-Crush
After: Crush
figure 3
A protein crystal before and after being bullied by a probe.
figure 5
Protein crystal stained with a dye.
figure 4
A weakly birefringent biological crystal.
Solutions for Crystal Growth
P A G E 2 - 2
salt or bi ologi cal crystals?
197
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Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
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250
200
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0
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2
2.5
3
3.5
4
4.1
4.2
4.3
4.4
4.5
4.6
4.7
4.8
4.9
5
5.1
5.2
5.3
5.4
5.5
5.6
5.7
5.8
5.9
6
6.1
6.2
6.3
6.4
6.5
6.6
6.7
6.8
6.9
7
7.1
7.2
7.3
7.4
7
.5
7
.6
7
.7
7
.8
7
.9
8
8
.1
8
.2
8
.3
8
.4
8
.5
8
.6
8
.7
8
.8
8
.9
9
9
.1
9
.2
9
.3
9
.4
9
.5
9
.6
9
.7
9
.8
9
.9
1
0
1
0
.1
1
0
.2
1
0
.3
1
0
.4
1
0
.5
1
0
.6
1
0
.7
1
0
.8
1
0
.9
1
1
1.0 1
1.5 1
2.0 0
2.5 1
3.0 7
3.5 17
4.0 46
4.1 5
4.2 32
4.3 11
4.4 12
4.5 78
4.6 39
4.7 10
4.8 21
4.9 16
5.0 102
5.1 8
5.2 51
5.3 28
5.4 37
5.5 98
5.6 53
5.7 21
5.8 23
5.9 24
6.0 239
6.1 9
6.2 54
6.3 24
6.4 29
6.5 217
pH Number of Crystals
6.6 38
6.7 46
6.8 116
6.9 35
7.0 379
7.1 21
7.2 78
7.3 32
7.4 77
7.5 251
7.6 45
7.7 13
7.8 49
7.9 6
8.0 220
8.1 3
8.2 15
8.3 14
8.4 18
8.5 79
8.6 7
8.7 8
8.8 8
8.9 3
9.0 32
9.1 2
9.2 2
9.3 2
9.4 1
9.5 19
9.6 0
9.7 1
9.8 8
9.9 0
10.0 7
10.1 0
10.2 1
10.3 0
10.4 0
10.5 2
10.6 0
10.7 0
10.8 0
10.9 0
11.0 1
pH Number of Crystals
pH Number of Crystals
pH versus number of crystals grown for 2,953 biological
macromolecules reported from the BMCD.
Solutions for Crystal Growth
1991-2009 Hampton Research Corp. All rights reserved. Printed in the United States of America.
This guide or parts thereof may not be reproduced in any form without the written permission of the publishers.
crystal growth vi a
p
h relaxati on
Try the following if sample solubility is pH dependent. Determine a pH and salt
concentration where the protein is insoluble. A simple way to do this, if sample
supply is limited, is to review old drops and look for those which contain pre-
cipitate. Review the data and select the reagent, reagent concentration, and pH
most often associated with precipitate. Or, if sample is abundant, using a micro-
scope, a microscope slide, or cover slide, empirically perform solubility tests by
adding 1 l of protein to 1 l of buffer (and/or precipitant) and watch the drop
for the formation of precipitate within a minute or so (not too long or the drop
will evaporate and you will always observe precipitate!). Once reagent and pH
points have been determined to promote a sample solubility minima, set hang-
ing or sitting drop vapor diffusion experiments with a smattering of the solubility
minima conditions. Once the sample has precipitated, add acetic acid (acidic) or
ammonium hydroxide (basic) to the drop (try 1 l of 0.1 M stock and adjust the
amount and/or concentration of the stock accordingly) until the precipitate is
redissolved. Seal the reservoir. The addition of the acid or the base will, in most
experiments, solubilize the precipitate. Since acetic acid and ammonium hydrox-
ide are volatile, the acidic/basic reagent will leave the drop. As the acidic/basic
reagent leaves the drop, the pH of the drop will relax to approximately the original
pH prior to the addition of the acidic/basic reagent. As the relaxation approaches
the original pH, the protein will approach a point of solubility minima and here is
when there is the chance for crystallization to occur. Variables to consider when
refining the pH relaxation technique include initial pH, buffer concentration, buf-
fer type, precipitant type, precipitant concentration, concentration and volume
of acetic acid/ammonium hydroxide added to the drop, reservoir volume, and
drop volume. One can also incorporate additives (organic solvents, chaotropes,
detergents, ions, salts, etc.) into the procedure to evaluate the effect of the addi-
tive on sample-sample and sample-solvent interactions. Sound tedious? Well, its
not as bad as it sounds. Approach the technique as both a crystallization experi-
ment and a crude solubility measurement. If no crystals are grown using the
method, all is not lost. One has at least obtained a great deal of pH and reagent
dependent solubility data which can be used to design additional crystallization
experiments. What should one do with the old experiments full of precipitate
and no crystals? Move the plates to warmer and then cooler temperatures. Note
changes in sample solubility. See a difference? Consider temperature relaxation
as another crystallization method.
p
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Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 198 Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
Solutions for Crystal Growth
n
Never use a pH probe containing AgCl when measuring the pH and/or titrat-
ing the pH of Tris and Tris HCl buffers since the AgCl will complex with the
Tris which can lead to erroneous pH readings. Use a Tris-compatible pH
probe when adjusting the pH of Tris buffers.
n
Follow the manufacturers recommended use and maintenance instructions
to keep the pH probe accurate and precise.
n
pH measurements are only as good as the probe.
n
Glass electrode probes are more precise and accurate than gel-filled plastic
probes.
n
Calibrate pH probes each and every day. Refresh calibration standard solutions
frequently to avoid contamination and evaporation.
n
A two-point calibration with the pH to be measured sandwiched between
the calibration points is better than a single or triple point calibration.
n
Bacterial contamination of pH standards, storage solution, and water can
cause pH fluctuations in reagents.
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1991-2009 Hampton Research Corp. All rights reserved. Printed in the United States of America.
This guide or parts thereof may not be reproduced in any form without the written permission of the publishers.
1991-2009 Hampton Research Corp. All rights reserved. Printed in the United States of America.
This guide or parts thereof may not be reproduced in any form without the written permission of the publishers.
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Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
Solutions for Crystal Growth
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7
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1
Num
ber of Crystals
500
450
400
350
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250
200
150
100
50
0
0
50
100
150
200
250
300
350
400
450
500
1 - 20 mg/ml
40
39
38
37
36
35
34
33
32
31
30
29
28
27
26
25
24
23
22
21
120
100
80
60
40
20
0
0
20
40
60
80
100
120
N
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rystals
21 - 40 mg/ml
60
59
58
57
56
55
54
53
52
51
50
49
48
47
46
45
44
43
42
41
30
25
20
15
10
5
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0
5
10
15
20
25
30
N
um
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rystals
41 - 60 mg/ml
80
79
78
77
76
75
74
73
72
71
70
69
68
67
66
65
64
63
62
61
7
6
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4
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1
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0
1
2
3
4
5
6
7
N
um
ber of C
rystals
61 - 80 mg/ml
1 23
2 76
3 80
4 97
5 392
6 78
7 60
8 86
9 27
10 483
11 18
12 35
13 21
14 14
15 127
16 15
17 10
18 16
19 3
20 159
21 3
22 5
23 4
24 1
25 51
26 0
27 5
28 3
29 3
30 110
31 0
32 2
[Protein] Number of Crystals
33 3
34 5
35 12
36 2
37 1
38 0
39 1
40 42
41 0
42 2
43 0
44 0
45 0
46 1
47 0
48 1
49 0
50 27
51 0
52 2
53 0
54 0
55 1
56 0
57 0
58 0
59 0
60 30
61 0
62 0
63 0
64 0
65 0
66 0
67 0
68 0
69 0
70 7
71 0
72 0
73 1
74 0
75 1
76 0
77 0
78 0
79 0
80 4
Number of Crystals
Number of Crystals
Protein concentration (mg/ml) versus number of crystals grown for 2,953
biological macromolecules reported from the BMCD.
[Protein]
[Protein]
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cryst alli zat i on polli ng booth
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Answer Percentage
4C only 3%
25C only 43%
4C and 25C only 33%
4C and 25C and other temperatures 21%
What temperatures do you routinely use for crystallization?
Answer Percentage
Very important
4%
Important 35%
45%
Neither important nor unimportant 7%
Slightly important
Not important at all
8%
How important is it to keep your chosen crystallization temperature constant
during the entire crystallization experiment (e.g. during crystal growth and
crystal inspection)?
Answer Percentage
Hanging Drop Vapor Difusion
Sitting Drop Vapor Difusion
Microbatch
Free Interface Difusion
Dialysis
31%
0%
0%
3%
What crystallization method do you use most often for crystal optimization?
66%
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Answer Percentage
Hanging Drop Vapor Difusion
Sitting Drop Vapor Difusion
Microbatch
Free Interface Difusion
Dialysis
36%
0.5%
0.5%
What crystallization method do you use most often for screening?
58%
5%
Answer Percentage
Cleave it (remove His tag) and screen
Leave tag intact and screen
Leave it & screen. No xtals, cleave & screen.
Based on your own experience, if your protein has a His tag, do you cleave
it or leave it when you screen for crystallization conditions?
59%
15%
26%
1991-2009 Hampton Research Corp. All rights reserved. Printed in the United States of America.
This guide or parts thereof may not be reproduced in any form without the written permission of the publishers.
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 202 Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
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Solutions for Crystal Growth
cryo qui cki es
Cryobuffer
Weve had good success using the well solution directly as the foundation of a
cryobuffer in several situations where crystals cannot be grown directly in the pres-
ence of cryoprotectant, and where crystals don't tolerate transfer to artificial mother
liquors. The basic protocol is as follows:
1. Remove 100 l of the well solution after crystals have grown.
2. Split this sample into two 50 l aliquots.
3. Add 7.5 mg of Dextrose (glucose) to the first aliquot and 15 mg of Dextrose to
the second. Dissolve by gently pipetting with a wide-bore tip. This will give
two sequential well solutions that now contain 15% and 30% w/v Dextrose. If
all the dextrose won't go into the second aliquot, spin hard and remove the
supernatant.
4. Transfer the crystal to aliquot 1, equilibrate for 3 minutes, then to aliquot
number 2, then freeze.
We've had a few crystals that routinely crack or blow up when transferred to
artificial mother liquor that behave well when transferred to well solution plus
glucose. We assume that there is some aspect of the crystal drop (pH, ionic ten-
sion, precipitant concentration) that is more effectively reproduced within the
well than by separately prepared mother liquors.
The nice thing about the protocol above is that you don't get much of a volume
increase when dry dextrose is dissolved in the well solution, so the components
in the solution are not diluted.
Finally, if you don't get a really good freeze, you can try to add about 5% v/v
Glycerol to aliquot 2 in addition to the 30% w/v Dextrose.
Thank you Barry Stoddard for sharing this tip.
Cryo Tip
When adding glycerol to crystallization experiments for cryogenic data collec-
tion, remember that some proteins are more soluble in glycerol and one might
have to increase the concentration of the primary precipitant (Example: Increase
Ammonium sulfate from 2.0 M to 2.1 M or 2.2 M in the presence of 20% v/v
Glycerol).
Cryo-Soaking Method
Eom et al. have reported improved diffraction using a novel cryo-soaking
method. Crystals were grown using the following condition: 1.8 M Ammonium
sulfate, 0.1 M Sodium citrate pH 5.1 (hanging drop, 0.5 ml reservoir). To improve
diffraction quality, the crystals were sequentially transferred to cryosolvents con-
taining 5 to 25% v/v Glycerol in five steps (5, 10, 15, 20, and 25%). Equilibration at
each step was 30 seconds. Note that the cryoprotectant (glycerol) also contains
the mother liquor used to grow the crystals. Equilibration at each step was 30
seconds. After the final transfer, the crystals were equilibrated for 30 minutes.
The cryosolvent containing the crystal(s) was then air dried until Ammonium
sulfate crystals began to appear. The authors report this took about 20 minutes at
room temperature. Diffraction resolution using the novel cryo-soaking method
improved from 3 angstrom to 2.2 angstrom. (Editors Note: Yet another case of
crystal dehydration?)
Reference:
Acta Cryst (1999) D55 Pages 1601-1603 Crystallization and preliminary X-ray crystallographic studies of the D2 region of the
skeletal ryanodine receptor. Seha Kim, Dong Wook Shin, Do Han Kim and Soo Hyun Eom. E-mail: eom@kjist.ac.ky
Crystal Annealing
Flash-cooling crystals can sometimes increase the mosaicity of biological macro-
molecular crystals. In some cases, macromolecular crystal annealing can reduce
the mosaicity of flash-cooled crystals without affecting molecular structure.
Crystal annealing involves cycling a flash-cooled crystal to room temperature and
then back to cryogenic temperature. The procedure can also be applied to some-
times restore diffraction from flash-cooled crystals that were not properly han-
dled to and from cryogenic storage. Crystal annealing does not seem to improve
a poorly diffracting crystal suffering from molecular disorder. The essential
features of the crystal annealing procedure are the following: The crystal is first
flash-cooled. The crystal is removed from the cryostream and quickly transferred
to a drop of cryoprotectant at crystal growth temperature and allowed to remain
in the drop for at least three minutes. The drop should be covered to prevent
evaporation. Finally, the crystal is remounted on a loop and flash-cooled.
Reference:
Macromolecular Crystal Annealing: Overcoming Increase Mosaicity Associated with Cryocrystallography, J.M. Harp, D.E.
Timm, and G.J. Bunick, Acta Cryst. (1998) D54 622-628.
Halides for Phasing Proteins
In the publication, Novel approach to phasing proteins: derivatization by short
cryo-soaking with halides, Dauter et al. described the procedure with four
different proteins including lysozyme, RNAase A, subtilisin, and xylanase. Hey!
Congratulations for taking the time to test beyond lysozyme! We wish more folks
would take the time to test novel crystallization discoveries with more than just
lysozyme! Anyway, Sodium iodide or Sodium bromide was added to the crystal-
lization reagent and cryoprotectant mixture for the derivatization. It was noted
that 1.0 M Sodium bromide did not seem to affect the quality and diffracting
power of the tested crystals. 1.0 M Potassium iodide did mess with the crystal-
lization of lysozyme and xylanase so the Potassium iodide concentration was
reduced to 0.35 and 0.5 M for these two proteins (CrystalNews Editor Note: Both
lysozyme and xylanase were crystallized in high salt where the other two proteins
were crystallized in MPD and PEG. So it seems one might need to adjust the
NaBr or KI concentration for high salt crystallizations and perhaps not too much
for polymer or non-volatile organic crystallizations. Who knows? More results
needed for a consensus). Crystals were transferred to the halide laced mother
liquor for 15 to 45 seconds. Crystals were then flash-cooled (you are welcome
Sean) for data collection. A fifth protein, in fact, a new protein to be described in
a future publication was also successfully tested with this novel protocol.
For more details about the identification of anamolous sites, the phasing, and
application to an unknown structure, see the following reference about quick
cryo-soaking with halides as a possible alternative method for phasing protein
crystal structures.
Reference:
Acta Cryst. 2000, D56, 232-237.
1991-2009 Hampton Research Corp. All rights reserved. Printed in the United States of America.
This guide or parts thereof may not be reproduced in any form without the written permission of the publishers.
how to:
cryocrystallography
and the crystalcap system
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Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
Solutions for Crystal Growth
Mounting Crystal
With the CrystalCap and
CryoLoop on the end of the
CrystalWand, remove a
crystal from the drop.
Place Crystal in Cryostream
Place the CrystalCap onto
the magnetic platform on the
goniometer head properly posi-
tioned in the cryostream.
Using Dewar to Freeze Crystal
Use the CrystalWand to immerse the
CrystalCap and CryoLoop containing the
crystal into a dewar containing liquid
nitrogen to freeze the crystal.
Storing Frozen Crystal
Immerse the CryoTong contain the
CrystalCap in a dewar contain-
ing liquid nitrogen. Place the
CrystalCap onto the vial and store
the crystal.
Use the Vial Clamp to hold the
CrystalCap vial and position the
CrystalCap into the vial.
Place the vial in a cryocane and
store the cryocane in a liquid
nitrogen storage dewar.
Using the CryoTong
Immerse the CryoTong in
liquid nitrogen and place
the CrystalCap into the
CryoTong.
Transferring the Crystal
Move the CryoTong from the
dewar and position the CrystalCap
onto the magnetic platform on the
goniometer head so the crystal is
positioned in the cryostream.
Removing Crystal and Storing
Immerse the CryoTong in liquid nitrogen. Place
the CryoTong over the CrystalCap and crystal.
Tilt and remove the CrystalCap from the goni-
ometer head.
Direct Freezing
Dewar Freezing
Storing Crystal
Transferring Crystal
Store Crystal
f l o w c h a r t f o r c r y o c o o l i n g o f c r y s t a l s
1991-2009 Hampton Research Corp. All rights reserved. Printed in the United States of America.
This guide or parts thereof may not be reproduced in any form without the written permission of the publishers.
Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com 204 Hampton Research Phone: 800-452-3899 or 949-425-1321 Fax: 949-425-1611 www. hampt onresearch. com Cust omer Servi ce: i nf o@hrmai l . com Techni cal Support : t ech@hrmai l . com
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usi ng hali des for phasi ng
molecular st ructures
Solutions for Crystal Growth
Preliminary research shows that bromide (as well as iodide) can diffuse into protein crystals when soaked with the appropriate solution and can successfully be
used for phasing.
1
Halide soaked crystals can be used for MAD, as well as multiple or single isomorphous replacement with anomalous scattering or for single
anomalous diffraction. The procedure has been termed Halide Cryosoaking by Dauter and Dauter.
1
In simplest terms, the procedure involves dipping the crystal
for a short period of time into a cryoprotectant solution that contains a significant concentration of halide salt. Although no single recipe will suffice for all proteins
since each crystal has a unique crystallization recipe and will require different cryoprotectant cocktails, there are some general suggestions to follow at this time.
First, there are currently more successful examples using bromide than iodide. The soak time is approximately 10 to 20 seconds. Longer soaks have not lead to
more incorporation of halide ions for the examples to date. The concentration range of sodium bromide for soaking is approximately 0.25 to 1 M. Higher concen-
trations of halide ions may lead to more sites with higher occupancies and increased phasing power. Factors influencing the success of the procedure include the
resolution and quality of the x-ray diffraction data, crystal symmetry, packing density, and pseudo-symmetric arrangements of molecules.
Tips for successful Halide cryosoak include:
1. Initially, preserve the formulation of the crystallization reagent used to grow the crystal as well as the formulation for a successful cryosoak and then
add the halide salt. In other words, leave everything constant and add the halide salt.
2. If the crystallization reagent contains salt, try substituting the halide salt, especially if the salt is Sodium chloride.
3. High concentrations of the halide salt can serve as a cryoprotectant without the addition of other traditional cryoprotectants (Glycerol, MPD, sucrose).
4. Experiment with soak conditions. Vary the concentration of the original reagents, the concentration of the halide salt, and the soak time.
Examples of successful crystallization reagents optimized for halide cryosoaking:
Original Condition (Black) Halide Cryosoak Condition (Blue)
1.0 M Ammonium sulfate, 5 mM guanidine, 10% Glycerol, 0.1 M Sodium citrate pH 3.3
3
1.0 M Ammonium sulfate, 5 mM guanidine, 18% Glycerol, 0.1 M Sodium citrate pH 3.3, 1 M Sodium bromide
3
1.4 M Lithium sulfate, 0.1 M Tris pH 7.5
3
1.2 M Lithium sulfate, 0.1 M Tris pH 7.5, 1 M Sodium bromide, 14% Glycerol
3
1.0 M Sodium chloride, 0.1 M Sodium acetate pH 4.7
2
0.1 M Sodium acetate pH 4.7, 1.0 M Sodium bromide, 30% Glycerol
2
50% MPD, 0.1 M Sodium acetate pH 5.4
2
50% MPD, 0.1 M Sodium acetate pH 5.4, 1.0 M Sodium bromide
2
12% PEG 4,000, 0.1 M Citrate, 1.0 M Sodium chloride, 10 mM Calcium chloride, pH 6.0
2
12% PEG 4,000, 10 mM Citrate, 10 mM Calcium chloride, 25% Glycerol, 1.0 M Sodium bromide
2
10% Ammonium sulfate, 0.1 M TRIS HCl pH 7.4
2
10% Ammonium sulfate, 0.1 M TRIS HCl pH 7.4, 25% Glycerol, 1.0 M Sodium bromide
2
References
1. Entering a new phase: Using solvent halide ions in protein structure determination. Dauter, Z. & Dauter, M., Structure, Vol 9, R21-26, Feb 2001.
2. Novel approach to phasing proteins: derivatization by short cryo-soaking with halides. Dauter, Z., Dauter, M., & Rajashankar, K.R. Acta Cryst. (2000) D56, 232-237.
3. Practical experience with the use of halides for phasing macromolecular structures: a powerful tool for structural genomics. Dauter, Z., Li, M. and Wlodawer, A.
1991-2009 Hampton Research Corp. All rights reserved. Printed in the United States of America.
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205
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Solutions for Crystal Growth
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Background
A crystallization experiment typically begins with the sample in a stabilizing
solution of water and a buffer, salt, reducing agent, ligand, or other reagent.
Prior to mixing the sample with crystallization reagent, this sample solution is
undersaturated with respect to the macromolecule in question (sample). In an
undersaturated sample solution, no crystals can nucleate, nor can crystals grow
from seeds. Upon addition of a crystallization reagent, the relative supersatura-
tion of the sample is increased. Assuming the crystallization reagent decreases
the solubility of the sample to increase the relative supersaturation, three events
can take place. In the first stage of supersaturation, the metastable zone, spon-
taneous homogenous nucleation cannot occur, but crystal growth from seeds
can occur. Moving further into supersaturation, the labile zone, spontaneous
homogeneous nucleation and crystal growth can occur. Further into supersatu-
ration, the precipitation zone, precipitation of the sample from solution occurs.
See figure 1 below.
Seeding
Seeding allows one to grow crystals in the metastable zone. Why would one
want to do this? For control, reproducibility, and to improve the likelihood of a
successful crystallization experiment. In the metastable zone, crystals can grow
from seeds but cannot spontaneously nucleate. By placing a seed or solution of