Name: liliacanaza

Exercise 1: Cell Transport Mechanisms and Permeability: Activity 2: Simulated Facilitated Diffusion Lab Report
Pre-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
1. Molecules need a carrier protein to help them move across a membrane because
You correctly answered: d. they are lipid insoluble or they are too large.
2. Which of the following is true of facilitated diffusion?
You correctly answered: c. Movement is passive and down a concentration gradient.
3. Examples of solutes that might require facilitated diffusion include
You correctly answered: d. all of the above.
4. Which of the following would not affect the rate of facilitated diffusion?
You correctly answered: a. the amount of intracellular ATP
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Experiment Results
Predict Question:
Predict Question 1: What effect do you think increasing the number of protein carriers will have on the glucose transport
rate?
Your answer : b. The glucose transport rate will increase.
Predict Question 2: What effect do you think adding Na+ Cl- will have on the glucose transport rate?
Your answer : c. There will be no change in the glucose transport rate.
Stop & Think Questions:
What happened to the glucose transport rate when the glucose concentration was increased from 2 mM to
8 mM?
You correctly answered: a. The glucose transport rate increased.
Describe the conditions that resulted in the fastest rate of glucose transport.
You correctly answered: d. 700 glucose carrier proteins and 8 mM glucose
Why do you think equilibrium wasn't reached?
You correctly answered: b. The membrane proteins were saturated.
Experiment Data:
Run Number Solute Start Conc. L Start Conc. R Carriers Rate
1 Na+ Cl- 0.00 0.00 500 0.0000
1 Glucose 0.00 2.00 500 0.0008
2 Na+ Cl- 0.00 0.00 500 0.0000
2 Glucose 0.00 8.00 500 0.0023
3 Na+ Cl- 0.00 0.00 700 0.0000
3 Glucose 0.00 2.00 700 0.0010
4 Na+ Cl- 0.00 0.00 700 0.0000
4 Glucose 0.00 8.00 700 0.0031
5 Na+ Cl- 0.00 0.00 100 0.0000
5 Glucose 0.00 10.00 100 0.0028#
6 Na+ Cl- 2.00 0.00 700 0.0042
6 Glucose 0.00 2.00 700 0.0010
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Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
1. What variable(s) increased the rate of facilitated diffusion of glucose?
You correctly answered: d. both a and b
2. For facilitated diffusion, increasing the concentration of glucose on one side of the membrane is the same as
You correctly answered: b. making the concentration gradient steeper
3. When all of the membrane carriers are engaged, or busy, we say they are
You correctly answered: a. saturated.
4. Na+ Cl- had no effect on glucose transport because
You correctly answered: b. sodium is not required for glucose transport in the simulation.
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Review Sheet Results
1. Explain one way in which facilitated diffusion is the same as simple diffusion and one way in which it is different from
simple diffusion.
Your answer:
Durante la difusin simple, las molculas tienden a atravesar la membrana desde regiones de alta concentracin hacia
regiones de baja concentracin, hasta alcanzar el equilibrio: igual proporcin de molculas atravesarn la membrana en
ambas direcciones.
- Difusin facilitada por protena de canal, algunas protenas de membrana forman canales que facilitan el paso de
sustancias polares o con carga elctrica. En este ejemplo de difusin facilitada, se muestra un canal especfico para el
pasaje de un in. El canal inico presenta una compuerta que puede abrirse o cerrarse por una variedad de
mecanismos.En este caso la apertura del canal depende de la participacin de una molcula que acta como seal.
En la difusin facilitada, al igual que en la difusin simple, la sustancia se mueve desde el lado de la membrana en donde
se encuentra en mayor concentracin hacia el otro lado de la membrana en donde la concentracin es menor. Este
movimiento a favor de gradiente de concentracin es espontneo y no requiere gasto de energa.
- Difusin facilitada por carrier, algunas protenas de membrana actan como carriers o transportadores de sustancias. El
carrier engancha a la molcula de un lado de la membrana, cambia su conformacin, depositando a la molcula del lado
opuesto. En este ejemplo de difusin facilitada, un carrier transportador de glucosa, mueve glucosa siguiendo su gradiente
de concentracin. El proceso no requiere energa.
2. The larger value obtained when more glucose carriers were present corresponds to an increase in the rate of glucose
transport. Explain why the rate increased. How well did the results compare with your prediction?
Your answer:
Porque cuanto mayor sea el nmero de proteinas transportadoras, mayor sera la velocidad de difusin facilitada
3. Explain your prediction for the effect Na+ Cl- might have on glucose transport. In other words, explain why you picked
the choice that you did. How well did the results compare with your prediction?
Your answer:
Por que los transportadores de glucosa y del cloruro de sodio son distintos, y especificos para cada uno, trabajan
independientemente, por eso la difusion facilitada de glucosa no vario, en los dos experimentos duro 33 minutos, sin alguna
modificacin por la adicion del cloruro sdico.