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!"""mL
! L
=#mL
# $Calculating concentration of dilute Gatorade 1olution u1ing [Euation ("
rea0ing ,ro. graph i+ appro-i.atel4 95" - !"
76
!
u+ing BeerLambert Law [Euation %" F 95$ - !"
76
'
%$ Calculating concentration of Allura Red in #2 oz of Gatorade u1ing [Euation ./"
8/here ("oz F "5G9 )
&$ Calculating mole1 of Allure Red in #2 oz of Gatorade
8/here ("oz F "5G9 )
'$ Calculating num/er of molecule1 of Allure Red in #2 oz of Gatorade
($ Calculating ma11 of Allure Red in #2 oz of Gatorade
.olar .a++ o, 2llure 3e0 ( %
!6
&
!
K
(
L
6
S
(
(7
) F G"5 g H .ol
DISCUSSION
*he purpo+e o, thi+ e-peri.ent /a+
to u+e +pe1trophoto.etr4 to 0eter.ine the
1on1entration o, 2llura 3e08 a ,oo0
1oloring 1o.poun0 o,ten u+e0 in
1o..er1ial +port+ beverage+ +u1h a+
Gatora0e5 :ro. the re+ult+ o, the
e-peri.ent it /a+ 0eter.ine0 that the the
1on1entration o, 2llure 3e0 in a (" oz
bottle o, Gatora0e i+ appro-i.atel4 65" -
!"
7$
'8 /hi1h i+ e;uivalent to (!( 9g o,
2llura 3e08 or (56 - !"
!$
.ole1ule+ o, the
1o.poun05 *he lo/ 1on1entration+
0eter.ine0 +ee. rea+onable8 in light o,
the potential health ri+<+ a++o1iate0 /ith
the 1on+u.ption o, +u1h 1o.poun0+ a+
0etaile0 in a report b4 the %enter ,or
S1ien1e in the Publi1 Intere+t @B5
2+ /ith an4 e-peri.ent potential +our1e o, error ari+e /hen a++u.ption are .a0e5 In thi+ e-peri.ent
an a++u.ption i+ .a0e that the ab+orban1e o, an a;ueou+ +olution o, 2llura 3e0 (a+ u+e0 ,or +tan0ar0+
in preparing the 1alibration 1urve) a0e;uatel4 repre+ent+ the ab+orban1e o, a 0ilute +olution o,
Gatora0e5 *hi+ o, 1our+e i+ not entirel4 a11urate a+ even a 0ilute +olution o, Gatora0e 1ontain+ +everal
f ("5"6) =
"5"6 "5""!$!G
6!6659!
= 95 * !"
6
!
95 * !"
6
! "5"G L
"5G9 L
=65" * !"
$
!
"5G9 L
65" * !"
$
mol
! L
=5$ * !"
$
mol
65"(( !"
(#
! mol
5$ * !"
$
mol
! L
= (56 !"
!$
molecules
5$ * !"
$
mol
G"5 #
! mol
!"
6
7#
! #
=(!( 7#
8i#ure 1 - S&ows Structural formula and ball and stic"
model of disodium ,-&9dro*9-1-'':-met&o*9-1-met&9l-
5-sulfop&en9l)a;o)-:-nap&t&alenesulfonate2 also "now
as Allura 3ed2 and 3ed 52 wit& a molecular formula of
<a:=1->15<:?-S:
other ingre0ient+ along /ith 2llure 3e0 an0 /ater5 >4 not 1alibrating the in+tru.ent to ta<e into
a11ount all po++ible ab+orber+8 a higher ab+orban1e rea0ing+ i+ .o+t li<el4 .ea+ure0 an0 thu+
1al1ulation+ /ill 4iel0 a po++ibl4 higher 1on1entration5 In or0er in1rea+e the a11ura14 o, the
.ea+ure.ent the %olori.eter /oul0 nee0 to 1alibrate0 to +ubtra1t the ab+orban1e ,ro. all other
potential ab+orber+ in the Gatora0e +olution e-1lu0ing the 2llure 3e05
2nother po++ible +our1e o, error to 1on+i0er i+ that volu.e in the e-peri.ent i+ treate0 a+ being
a00itive8 /hen the .i+1ibilit4 o, the t/o +olution i+ un<no/n an0 the re+ulting 0ilution+ .a4be not be
ver4 pre1i+e5 *hi+ i.pa1t+ the +ub+e;uent a11ura14 o, the 1on1entration+ 0eter.ine05 &o/ever given
the +.all 1on1entration+ u+e0 ,or the +tan0ar0 +olution+8 it i+ po++ible the error in1urre0 i+ largel4
in+igni,i1ant8 thu+ it+ not e-pe1t to have ha0 an4 +igni,i1ant i.pa1t on the re+ult+5 :inall4 there i+
al/a4+ the 1on+i0eration ,or hu.an error an0 ina11ura14 an0 pre1i+ion in te1hni;ue /hi1h 1an o,ten be
eli.inate0 /ith .ore trial+5
CITATION
@!B "eer#ambert la$. (201%& 'ovember 22!. (n Wikipedia, The Free Encyclopedia. Retrieved 21)08& 'ovember 24&
201%& from *tt+)//en.$i,i+edia.or-/$/inde..+*+/title0"eer12218013%ambert4la$5oldid058282%045
@(B ChemLab - Chemistry 6 - Week 4 - The Spectra of Conjugated Dyes and Inestigation of !eer"s La#.
(2011, September 20). Retrieved November 11, 2013, from
http://www.dartmouth.edu/~chemab/chem!/d"e#/fu$te%t/chemi#tr".htm
@#B $fficia% Site for &epsiCo !eerage Information ' Co%ors. (2013, &ctober 2'). Retrieved November 2(,
2013, from http://www.pep#icobevera)efact#.com/coor#.php
@B *e+ter for Scie+ce i+ the ,ubic -+tere#t (2010). (ood Dyes) * +ainbo# of +isks (.). Retrieved from
http://c#pi+et.or)/+ew/pdf/food/d"e#/rai+bow/of/ri#0#.pdf
8i#ure 1 - S&ows Structural formula and ball and stic" model of disodium ,-&9dro*9-1-'':-met&o*9-1-
met&9l-5-sulfop&en9l)a;o)-:-nap&t&alenesulfonate2 also "now as Allura 3ed2 and 3ed 52 wit& a
molecular formula of <a
:
=
1-
>
15
<
:
?
-
S
: