Variations in insecticidal activity and chemical compositions of leaf essential oils

from Cryptomeria japonica at different ages

Sen-Sung Cheng
a
, Meng-Thong Chua
b
, Ed-Haun Chang
c
, Chin-Gi Huang
d
, Wei-June Chen
e
,
Shang-Tzen Chang
b,

Abstract
The larvicidal effects of the essential oils extracted from the leaves of Cryptomeria japonica at
different ages (58, 42, and 26 years old) against 2 mosquito species, Aedes aegypti and Aedes
albopictus, were studied. The analysis of major constituents of these essential oils was also
investigated. Results obtained from the larvicidal tests, using essential oil from the leaves of 58-
year-old C. japonica was found to be most effective against both A. aegypti and A.
albopictus larvae, indicating tree age has significant influence on mosquito larvicidal activity. In
addition, the eleven pure constituents from C. japonica leaf essential oil were also tested
individually against the two mosquito larvae. Among them, -terpinene, -terpinene, p-cymene,
3-carene, terpinolene, and -myrcene shows strong larvicidal effect against the two mosquito
larvae. Among these pure constituents, 3-carene exhibits the best larvicidal effect against A.
aegypti and terpinolene shows an excellent inhibitory action against A. albopictus larvae. The
results of this study show that the leaf essential oil and its effective constituents might be
considered as a potent source for the production of fine natural larvicides.
Keywords
Cryptomeria japonica; Aedes aegypti; Aedes albopictus; Essential oils; Mosquito larvicidal
activity

















Biochemical Systematics and Ecology
Volume 37, Issue 4, October 2009, Pages 470473

Chemical composition and larvicidal effects of essential oil ofDendropanax
morbifera against Aedes aegypti L.
Ill-Min Chung
a
, Su-Hyun Seo
a
, Eun-Young Kang
a
, Sun-Dong Park
b, d
, Won-Hwan
Park
c, d,
Hyung-In Moon
d, e,

Abstract
Essential oils obtained from the flowers of Dendropanax morbifera were extracted and the
chemical composition and larvicidal effects were studied. The analyses were conducted by gas
chromatography and mass spectroscopy (GCMS) revealed that the essential oil of D.
morbifera contained 27 compounds. The major chemical components identified were -elemene
(18.59%), tetramethyltricyclohydrocarbon (10.82%),-selinene (10.41%), -zingibirene
(10.52%), 2-isopropyl-5-methylbicylodecen (4.2%), -cubebene (4.19), and 2,6-bis(1,1-
Dimethylethyl)-4-phenol (4.01%). The essential oil had a significant toxic effect against early
fourth-stage larvae of Aedes aegypti L. with an LC
50
value of 62.32 ppm and an LC
90
value of
131.21 ppm. The results could be useful in search for newer, safer, and more effective natural
larvicidal agents against A. aegypti.
Keywords
Aedes aegypti; Dendropanax morbifera; Essential oils; Larvicidal effects














Chemical compositions and larvicidal activities of leaf essential oils from two eucalyptus
species
Sen-Sung Cheng
a
, Chin-Gi Huang
b
, Ying-Ju Chen
c
, Jane-Jane Yu
d
, Wei-June Chen
e
, Shang-Tzen
Chang
Bioresource Technology
Volume 100, Issue 1, January 2009, Pages 452456
Abstract
In the current study, the mosquito larvicidal activity of leaf essential oils and their constituents
from two eucalyptus species (Eucalyptus camaldulensis and Eucalyptus urophylla) against two
mosquito species,Aedes aegypti and Aedes albopictus, was investigated. In addition, the
chemical compositions of the leaf essential oils were analyzed using gas chromatographymass
spectrometry. Results from the larvicidal tests revealed that essential oil from the leaves of E.
camaldulensis had an excellent inhibitory effect against both A. aegypti and A. albopictus larvae.
The 12 pure constituents extracted from the two eucalyptus leaf essential oils were also tested
individually against two mosquito larvae. Among the six effective constituents, -terpinene
exhibits the best larvicidal effect against both A. aegypti and A. albopictus larvae. Results of this
study show that the leaf essential oil of E. camaldulensis and its effective constituents might be
considered as a potent source for the production of fine natural larvicides.
Keywords
Eucalyptus camaldulensis; Eucalyptus urophylla; Essential oils; Aedes aegypti; Aedes albopictus
Larvicidal activity of Tagetes patula essential oil against three mosquito species.
Bioresource Technology Volume 96, Issue 11, July 2005, Pages 12351240
Abstract
Larvicidal activity of Tagetes patula essential oil was tested against the fourth instar
larvae of Aedes aegypti,Anopheles stephensi, and Culex quinquefaciatus. Five different
concentrations of essential oil were studied and the results were compared with that of
synthetic insecticide, malathion. A. aegypti (LC50 13.57, LC9037.91) was most
susceptible followed by An. stephensi (LC50 12.08, LC90 57.62) and C.
quinnquefaciatus(LC50 22.33, LC90 71.89).
Keywords
Aedes, Anopheles; Culex; Essential oil; Larvicidal; Tagetes patula

Bioresource Technology
Volume 100, Issue 7, April 2009, Pages 22842288
Chemical composition, oviposition deterrent and larvicidal
activities against Aedes aegypti of essential oils from Piper
marginatum Jacq. (Piperaceae)
E.S. Autran
a
, I.A. Neves
b
, C.S.B. da Silva
a
, G.K.N. Santos
a
, C.A.G. da Cmara
b
, D.M.A.F. Navarro
a,


Abstract
The essential oils of leaves, stems and inflorescences of Piper marginatum, harvested in the Atlantic
forest in the State of Pernambuco, Brazil, were obtained by hydrodistillation. GC and GCMS analyses
revealed the presence of 40 components accounting, respectively, for 99.6%, 99.7% and 99.1% of the
leaf, stem and inflorescence oil, the most abundant being (Z)- or (E)-asarone and patchouli alcohol. The
essential oil of the inflorescences exhibited potent activity against the 4th instar of Aedes aegypti with
LC10 and LC50 values of 13.8 and 20.0 ppm, respectively. Furthermore, the inflorescence oil did not
interfere in the oviposition of A. aegypti females when assayed at 50 ppm. These properties suggest
that P. marginatum oil is a potential source of valuable larvicidal compounds for direct use or in
conjunction with baits in traps constructed to capture eggs and larvae.
Keywords
Piper; Aedes; GCMS analysis; Larvicide; Oviposition deterrent








Journal of Asia-Pacific Entomology
Volume 13, Issue 2, June 2010, Pages 107109
Chemical composition and larvicidal activity of leaf essential
oil from Clausena dentata (Willd) M. Roam. (Rutaceae) against
the chikungunya vector, Aedes aegypti Linn. (Diptera:
Culicidae)
Sankaran Rajkumar
a,
Arulsamy Jebanesan
b


Abstract
Larvicidal activity of essential oil and isolated compounds from Clausena dentata leaves were tested
against early fourth instar Aedes aegypti larvae. GCMS analysis of essential oil revealed the presence of
fourteen compounds of which the major compounds were sabinene (21.27%), biofloratriene (19.61%),
borneol (18.34%) and -bisabolol (17.68%). The essential oil of C. dentata exhibited significant larvicidal
activity, with 24 h LC50 and LC90 values of 140.2 and 341.6 mg/l, respectively. Larvicidal activities of the
four major compounds of essential oil were also tested. The LC50 values of sabinene, biofloratriene,
borneol and -bisabolol were 27.3, 47.4, 43.5 and 33.2 mg/l, respectively. Results of this study show that
the leaf essential oil of C. dentata and its four major compounds may be a potent source of natural
larvicides.
Keywords
Aedes aegypti; Clausena dentata; Essential oil; GCMS analysis; Larvicide