USERS MANUAL

Microbiological Safety Cabinet BIOULTRA

BIOULTRA_I_ver0106US.doc

Telstar Industrial, S.L. Josep Tapiolas, 120 08226 TERRASSA (Espaa) Tel. +34 937 36 16 00 Fax: +34 937 85 93 42 telstar@etelstar.com www.etelstar.com

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SUMMARY
0. 1. SYMBOLS AND ABBREVIATIONS______________________________________ 3 GENERAL DATA___________________________________________________ 3

2. DESCRIPTION OF THE SAFETY CABINETS ______________________________ 4 2.1. working principle _____________________________________________ 4 2.2. Applications and principle diagram ________________________________ 4 2.3. Components description ________________________________________ 5 2.4. standard equipment ___________________________________________ 5 2.5. Certification _________________________________________________ 5 2.6. Technical features_____________________________________________ 5 3. INSTALLING INSTRUCTIONS ________________________________________ 6 3.1. Transport ___________________________________________________ 6 3.2. Cautions for first connection _____________________________________ 8 3.3. Electrical connection and services_________________________________ 8 3.4. Cabinet ductwork _____________________________________________ 9 3.5. Certification or installation test___________________________________ 10 4. OPERATING INSTRUCTIONS _________________________________________ 11 4.1. Start-up ____________________________________________________ 11 4.2. Front panel positions(additional keyboard)__________________________ 11 4.3. General Working recommendations _______________________________ 12 4.3.1. Ergonomics ______________________________________________ 13 4.4. Definition of the work zone______________________________________ 16 4.5. Remote control case and menus__________________________________ 16 4.6. Disconnection ________________________________________________ 21 4.7. Prolongated stoppage __________________________________________ 21 4.8. Optional accessories ___________________________________________ 22 5. MANTEINANCE ___________________________________________________ 22 5.1. Maintenance and control table ___________________________________ 22 5.2. Absolute filters _______________________________________________ 23 5.3. Spare parts __________________________________________________ 24 6. CLEANING AND DISINFECTION ______________________________________ 24 6.1. Surface cleaning and disinfection. ________________________________ 24 6.2. Glass front cleaning and disinfection ______________________________ 24 6.3. Disinfection with formaldehyde___________________________________ 24 7. 8. MAINTENANCE CHART _____________________________________________ 24 ELECTRIC DIAGRAMS ______________________________________________ 24

9. TESTS __________________________________________________________ 25 9.1. Impulsion filters integrity Test ___________________________________ 25 9.2. Exhaust filters integrity Test_____________________________________ 25 9.3. Inhalation leak Test (only CYTOULTRA model) _______________________ 25 9.4. Downflow air velocity Test ______________________________________ 25 9.4.1. Set point alarm downflow air velocity Test ______________________ 25 9.5. Inflow air velocity test _________________________________________ 25 9.5.1. Set point alarm inflow air velocity test _________________________ 25 9.6. Smoke test __________________________________________________ 25 9.7. Light test ___________________________________________________ 25 9.8. NOISE level test ______________________________________________ 25 10. POSSIBLE PROBLEMS AND SOLUTIONS ________________________________ 26

11. CERTIFICATES ___________________________________________________ 29 11.1. CE _________________________________________________________ 29 11.2. Guarantee ___________________________________________________ 29

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0. SYMBOLS AND ABBREVIATIONS

List of stickers used in the biological cabinets. EARTH WIRE PROTECTED STICKER.

FUNCTIONAL EARTH WIRE STICKER.

BIOHAZARD STICKER.
B IO H A Z A RD

CYTOTOXIC HAZARD STICKER.

CYTOTOXIC

CERTIFIED ACCORDING

CLASS II CABINET CERTIFIED ACCORDING TO EN-12469-2000 STICKER.

MACHINE CERTIFIED BY TV STICKER.

Abbreviations used in this manual

V A W, kW, W mm, cm, m kg

Volts Amperios Wats, kilowatts, microwatts Milimetres, centimetres, metres Kilogram

s, h Hz dB C

Seconds, hours Herzius Decibel Degrees

1. GENERAL DATA
Operating Instructions Manual for Microbiological Safety Cabinets Class II, BIOULTRA STANDARD, which ensure (applying correct techniques in laboratory) high level protection for the operator, the environment and the sample.

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WARNING This Manual must be read and understood before its installation, maintenance and operation of the biological safety cabinet Class II. All the safety instructions given in the Manual must be followed. Any use or application failing to comply with the recommendations expressed in the Manual may cause danger and loss of rights to manufacturing guarantee as well as freeing the manufacturer from all responsibility arising. Any maintenance must be done by qualified staff according to the laboratory working correct conditions. In case these actions are not carefully carried out, as a result the safety cabinet can work in abnormal conditions. All maintenance actions on this equipment must be performed by a qualified technician who is familiar with the proper maintenance procedure required for this equipment. MANUFACTURER : TELSTAR INDUSTRIAL, S.L. Jos Tapiolas, 120 08226 TERRASSA (ESPAA) Telf (+34) 93 736 16 00 Fax (+34) 93 785 93 42 e-mail: telstar@etelstar.com http://www.etelstar.com

2. DESCRIPTION OF THE SAFETY CABINETS

2.1. WORKING PRINCIPLE

CLASS II Biohazard Safety Cabinets are designed for microbiology work with the most advanced microprocessor control, ensuring a high level of protection for the operator, environment and sample. Cabinet exterior in laminated, lacquered, oven-dried stainless steel of a functional design with reduced external measurements and front access for changing filters. Spacious internal chamber with automatic sliding front made from laminated safety glass. Segmented work surface and liquid collection tray in polished stainless steel. Three-fan system: two for the laminar down flow and a third for exhaust, providing double security. In the case of a breakdown of one of the laminar down-flow fans, the other one continues to provide airflow to the laminar flow while the exhaust fan slightly increases its volume to ensure good depression in the work zone. In the event of both the laminar downflow fans failing, the exhaust fan can increase its volume enough to provide the depression needed. As can be seen in Annex A2, the air propelled by the laminar down-flow fans (1) is unloaded into the chamber or plenum (3) and, via the Absolute HEPA filter (5), is filtered and enters the laminar regime in the work zone (7), obtaining Class 100 according to US Fed. Std. 209D. The exhaust air is expelled by the fan (2) via a second plenum (4) and Absolute HEPA filter (6) to the outside (9). System of recycled, sterile vertical laminar flow in depression of 70% and exhaust of 30% of sterile air, approximately.

2.2.

APPLICATIONS AND PRINCIPLE DIAGRAM

The MSC models are appropriate for handling biologically pathogenic samples or products (up to level-3 risk micro organism), ensuring Class 10 protection of sterile air in the work zone, as well as a high level of protection for the operator and the environment. Principle diagram in Annex A1.

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WARNING Under no circumstances, is possible handling in corrosive, inflammable or explosive gas ambient. It is recommended that extracted air should be expelled to the exterior. Right works conditions are obtained only when the in and out blowing fans green LEDs are on (green colour).

2.3.

COMPONENTS DESCRIPTION

(See Annex A2)

2.4.

STANDARD EQUIPMENT

6A - 1380W socket distributed in two inlets on either side of the work zone. Insulation level IP44 IK07. Easy-to-work, microprocessor-controlled UV germicidal lamp with a timer function. Can only be enabled from the Disinfection display (Section 4.5 point 4.2). Average disinfection intensity over the work zone of 200 W/cm2 with a 20% depreciation after a working life of 9000 hours.

WARNING In the event of using this accessory, you should consider the normal precautions in the use of these types of lamps. Their use is only suitably for previous external sterilisation of materials introduced in the cabinet. That is why the lamp is a previous accessory to working in the cabinet and it is essential to keep it turned off when the operator is in front of the table or working on it. SECURIT glass provides protection from direct radiation but UV rays are reflected on polished metal parts and can seriously damage an operators eyes. That is why the glass front automatically slides into the closed position and the fans disconnect when the UV light is connected.

2.5.

CERTIFICATION

Quality control and results certification of trials carried out in line with standards. Certificate on the inside of the cabinet.

2.6.

TECHNICAL FEATURES
Characteristics Units BIOULTRA 1400 x 795 x 1430. (mm) LxWxH (mm) 1190 x 565 x 650

External dimensions (LxWxH) Internal dimensions

Front opening height Weight Laminar flow velocity air / Laminar flow

cm. Kg m/s m3/h

200 200 0,40 / 1064

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Front velocity air inlet / Exhaust flow Power supply Lighting Noise Vibration Tension Filters HEPA/ULPA Fans H 14

m/s m3/h KW Lux dB (A) Mm RMS V

0,55 / 540 2,1 kW > 750 < 60 < 0,005 230 Filtering system in the exhaust and the impulsion 99,999% D.O.P. Three high-efficiency fans two in laminar flow and the other in the extraction. Flows measured by thermoanemometric probes.

Microbiological Safety Cabinet BioUltra, 50Hz, 230V DISSOCIATED DECLARED VALUES OF NOISE EMISSION Standard(s) to which conformity is declared ISO 4871 Vdownflow = 0,4m/s Vinlet = 0.6 m/s LpA (dB) K pA (dB) 55.3 2

Certain values according to acoustic test given in standard EN 12469:2000 using standard Norma ISO 11201

3. INSTALLING INSTRUCTIONS
For a correct start-up procedure of the MSC, observe the following step-by-step instructions.

3.1.

TRANSPORT

The cabinet is supplied in an individual package and in the suitable way to prevent it from being damaged

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WARNING For any handling during assembly or dismantling of the cabinet, appropriate safety measures, such as wearing safety gloves or shoes, should be taken. a) Cabinets should be placed away from entrances, corridors, ventilation supply points, etc., which might affect airflow in the work zone. b) Try to ensure the cabinet is set perfectly on the table or workbench in order to prevent vibrations. c) To stop the work zone from overheating, we recommend the room temperature should fall within the comfort margins for working. The optimal comfort margins are: Temperature: 17-28C. Relative humidity: less than 90%.

If either of these conditions is not met, optimum cabinet performance cannot be guaranteed. d) When necessary, air vents in the room should be modified or speed of air entry reduced so that air is not aimed directly at the cabinet, as this may produce turbulence. Side air vents are not appropriate for rooms where cabinets are installed. e) Given that the ceiling temperature affects air distribution, sites should be selected with enough space. We recommend leaving a 500mm space between the exhaust and the ceiling. f) The site of the cabinet will directly influence the noise level. This level will rise considerably in small spaces with very reflective surfaces because of the proximity of the cabinets to the walls, and where fan speed has risen to compensate for air currents in the room or the resistance of clogged filters.

g) The site should allow disinfection gases with formaldehyde (Annex A5.2) to be expelled into the atmosphere. h) Correct cabinet performance should be tested before putting it into operation if changes have been made to the room or to the air intake system which may considerably alter the airflow characteristics in the room. i) We recommend positioning the cabinet in such a way that it makes it hard to accidentally disconnect it, e.g., next to a wall or another piece of equipment.

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3.2.

CAUTIONS FOR FIRST CONNECTION

Before connecting it to the mains supply, give the cabinet a clean to eliminate any dust particles that may have accumulated during transportation. Follow the instructions on cleaning and disinfection. Before using it for the first time, leave the cabinet running for around six hours to purge the filters. New filters give off a characteristic smell which tends to disappear in a short period of time.

WARNING The cabinet should work at the voltage shown on the characteristics plate. It should be connected to a suitable socket base (minimum 16A) with an earthed plug. The cable section recommended for the base is 2.5 mm.

EARTH WIRE

3.3.

ELECTRICAL CONNECTION AND SERVICES

The electrical characteristics of the cabinet are as follows: Electrical class: Class I. Insulation level: IP-20.

The cabinet is supplied with single-phase electro-fans and a connection lead with a European plug (other connections available as optional accessories). The on/off button is in the remote-control case (Annex A3 pos. 1). The power point in the wall should be sited in a perimeter around the cabinet of: 1.25 m from the centre of the cabinet to the sides 2.5 m from the base of the cabinet upwards Voltage input (1) Checking valve for D.O.P. control (2) Liberating screws and opening electrical drawer lid (3) you can accede to gas sockets (4) and vacuum ones (5).

On the top right-hand side of the cabinet and identified by name are:

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WARNING

The cabinet can only be activated from the

button in the remote-control case.

In the event of a power failure the cabinet will stop, showing the Power Failure alarm and automatically closing the front. Remove hands from the work zone when power failure occurs. The installation to which the cabinet is connected should meet the safety requirements in force in each country. Exhaust air should be evacuated outside the lab if working with volatile substances not neutralised by absolute filters. During normal work in these safety cabinets and because of a basic principle of recirculation and depression in the work zone, no perforated surface in the work zone should be covered with paper or sanitary fabric.

3.4.

CABINET DUCTWORK

If a duct is installed to evacuate the sterile exhaust air outside, it should not be more than 4 metres long. If the duct has to be longer, it should have a larger diameter or a supplementary exhaust fan should be installed at the end of the run. We recommend consulting the distributor or manufacturer. In the event of installing an additional fan, a potentiometer should be connected to regulate its speed in order to synchronise the exhaust airflow from the duct with the exhaust airflow from the cabinet. We also recommend interconnecting the exhaust fan and the cabinet on/off button so that it turns on and off together with the cabinet.

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WARNING In the event of having to install an additional fan, we recommend positioning it at the end of the run outside the lab facilities so that the exhaust duct is always under negative pressure. The outlet of the exhaust duct to outside should be duly protected against the entry of air currents, insects and small animals.

3.5.

CERTIFICATION OR INSTALLATION TEST

According to standard EN 12469 and in order to ensure correct cabinet operation, TELSTAR recommends that after the installation and connection of the cabinet, the following test should be done (before the equipment is used for the first time): 9.1. Impulsion filters integrity Test. 9.2. Exhaust filters integrity Test. 9.3. Test for leaks in the aspiration filter (CytoUltra model only). 9.4. Downflow air velocity. 9.4.1. Set Point alarm downflow air velocity Test. 9.5. Inflow air velocity Test. 9.5.1. Set Point alarm inflow air velocity Test. 9.6. Smoke test. 9.7. Light Test. 9.8. Noise Level Test

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Telstar, its representatives and distributors put a qualified technical team at your disposition that uses control equipment with maximum health protection for carrying out this installation test under full safety guarantees.

4. OPERATING INSTRUCTIONS
4.1. START-UP

Once the above points have been controlled, start up the MSC by pressing the On/Off button (Annex A3, pos. 1). After entering the password correctly (or immediately if this function has been disabled), the fans will automatically connect and the front will move into the work position (Annex 1.2). Ensure that the aspiration zone and air exhaust outlet are free (Annex A2 pos. 8 and 9). The electronic system of regulation by microprocessor via 2 thermoanemometer probes, laminar down flow and exhaust airflow, needs a few seconds to stabilise during connection in order to begin to work normally. The cabinet can only be used by qualified staff who meet the correct work techniques in the laboratory. If these techniques are not respected, faulty working of the equipment could result.

4.2.

FRONT PANEL POSITIONS. ADDITIONAL KEYBOARD

The front can be moved independently of the main control display by using the additional keyboard located on the right-hand side of the work zone.

The additional keyboard works the same as the positioning keys on the front of the main control (Section 4.5, point 3).

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First press: completely opens the front. Second press: stops the movement of the front.

First press: moves the front into the work position. Second press: stops the movement of the front.

First press: completely closes the front. Second press: stops the movement of the front.

The additional keyboard is enabled whenever the cabinet is connected to the mains supply (even when the cabinet is turned off) and is not blocked (Section 4.5, point 4.2). This makes it possible to move the front for cleaning and maintenance work without having to know the user password.

4.3.

GENERAL WORKING RECOMMENDATIONS

a) We recommend starting up the cabinet around 10 minutes before you begin to work. This will ensure particles are swept from the work zone and from material introduced. b) Wash your arms, hands and nails well, using an anti-bacterial soap, before and after work. Staff should avoid touching their mouths and eyes. c) We recommend wearing lab coats with adjusted cuffs and, in special works, protection gloves (or sleeve cuffs). Both the coat and cuffs should be made from a fabric that minimises the emission of fibres and particles.

d) The work zone inside the cabinet should not be used to store lab equipment. This can produce an accumulation of dust that is unnecessary and hazardous for working under sterile conditions. The fewest pieces of equipment possible should be positioned in order to work correctly and with the minimum number of interruptions for the user (inserting and removing arms from the work zone). e) Although the work zone is under a clean and sterile laminar down flow, the area around the cabinet might be contaminated. You should know the sources of contamination to prevent them when inserting arms, material, etc. f) All the material needed for the work should be free of particles and carefully cleaned before it is entered.

g) Materials such as paper, wood, cardboard, pencils, rubbers, etc. should not be entered in the work zone as they shed a great many particles. h) In the event of using pipettes, they should be of a mechanical aspiration type and you should never pipette by blowing with your mouth because it is easy to inhale aerosols caused buy suction. i) When platinum handles are used, it is advisable to use electric incinerators, preferably single-use ones. If the work requires the use of a gas flame from a Bunsen burner or similar, we recommend using a type where the flame is maintained by holding down a button. Please note that use of a constant flame burner creates significant turbulence. Bear in mind that too large a flame could burn the absolute filters.

j)

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k) Shadows and turbulence provoked by objects, equipment and materials sited in the work zone should be studied before work commences in order to evaluate their possible effects. Bear in mind that the laminarity of the airflow does not recover until a distance of 2.5 times the diameter of the object that causes the obstruction. l) When using bottles and tubes, they should preferably have a screw top instead of cotton stoppers which easily shed a large number of particles.

m) When the work is finished, all disposable products (handles, Petri dishes, etc.) as well as means of cultivation, samples, tubes, bottles, etc. should be removed from the cabinet in impermeable bags suitable for sterilisation if necessary. n) When working in the cabinet and during cleaning operations, it is important to prevent damage to the HEPA filters occasioned by knocking them, projecting liquids or splashing them, etc. o) All equipment connected to the base inside the cabinet should be fitted with an earth wire and meet all the electrical specifications. p) People traffic generates air movements that may cause unexpected loss to the containment barrier and, in the case of Class II cabinets, a reduced level of product protection. Warning signs and other means should be used to prevent traffic during critical operations. Cabinet sites can be divided to reduce the effects of air movement produced by other cabinets and people traffic. q) Suddenly opening and closing doors may disturb airflows in the cabinet and should be avoided when cabinets are being used.

WARNING 1.- The UV light system should only be used when the front is closed. 2.- Never connect equipment whose consumption exceeds that indicated on the earth wire inside the cabinet.

4.3.1.

ERGONOMICS

Microbiological security cabinet BioUltra and its accessories have been designed following recommendations and specifications of the standard EN ISO 14738:1999. Users should consider their posture when working with the cabinet, for their own safety. Below are examples of four postures NOT recommended and one that IS recommended.

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Fig. 1

Fig. 2

Fig. 3

Fig. 4

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Adjusting the seat and adopting a correct posture enables the worker to work safely and to prevent any possible physical damage (sight, neck, back, etc.)

Fig. 5
Also, as protection for both the product and the user, it is necessary that the cabinet is sited in a place without air currents, which may cause turbulence in the laminar down flow.

Fig. 6

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4.4.

DEFINITION OF THE WORK ZONE

To prevent creating turbulence in the laminar down flow and to protect the user and product, it is necessary to establish a work zone inside the cabinet. Do not work in any area with perforations and do not cover them up. Work 10 to 15 cm from the edges of the cabinet.

Fig. 7

4.5.

REMOTE CONTROL CASE AND MENUS.

The following elements are found in the remote-control case:

A. On/Off Button

(Annex 3 pos. 1):

Pressing when the cabinet is turned off: Accesses the Password Request display. After entering the correct password (Section 4.5 point 1), access the Main Operation display. If you have disabled the password request (Section 4.5 point 5) it will go straight to the Main Operation display. Pressing when the cabinet is turned on: Accesses the Password Request display. After entering the correct password (Section 4.5 point 1), the cabinet will switch off. If you have disabled the password request (Section 4.5 point 5) it will switch off straight away.

B. 100% Exhaust Button

(Annex 3 pos. 2):

The exhaust fan raises its airflow to the limit to ensure maximum user protection.

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C. Electrically operated gas valve connected LED

(Annex 3 pos. 3):

Indicates that the electrically operated gas valve is connected, enabling gas to pass to the tap of the work zone.

D. Plug enabled LED

(Annex 3 pos. 4):

Indicates that the plugs in the work zone are enabled, so that current can be supplied to any electrical device connected to them.

E.

Luminaire/UV lamp enabled two-colour LED

(Annex 3 pos. 5):

Indicates through the use of two different colours when the luminaire or UV lamp is connected.

F.

Fans connected LED

(Annex 3 pos. 6):

Indicates that the automatic airflow regulation system of the impulsion/exhaust fans is connected and that they are working. G. Backlit graphic display (Annex 3 pos. 7): Display that shows alarm messages, operating graphs, the value of the function buttons and all the information required in cabinet-user communication. H. Function buttons (Annex 3 pos. 8): Buttons whose function varies according to the display at which the user is situated.

I.

Menu button

(Annex 3 pos. 9):

Enables the user to move through the different system displays.

Below is an explanation of the different system displays: 1. Password Request Display. The four function buttons are enabled for password entry: - F1 - F2 Increases the value of the selected field (_) Confirms the value elected in a field and moves the selection to the next field. After pressing for the final time, if the password is correct, it accesses the Main Operation display. Reduces the value of the selected field (_) Provides access to the Language Selection display. Only appears after the first connection to the mains supply. If you want to change language again you will have to disconnect and reconnect the cabinet to the mains.

- F3 - F4

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- F4

Provides access to the Cleaning display. Appears after the second and subsequent connections.

1.1 Language Selection Display. The different languages available are assigned to a function button: - F1 - F2 - F3 - F4 Spanish. English. German. French.

Once you have selected the language, use the MENU button (Annex 3 pos. 9) to access the Password Request display. 1.2 Cleaning Display. This display enables you to access the cabinet to clean it without requiring the presence of authorised staff:

- F1 First press: completely opens the front. Second press: stops the movement of the front. (The same for the additional keyboard). - F2 - F3 Emergency stop. First press: completely closes the front and stops the fans. Second press: stops the movement of the front. (The same for the additional keyboard).

- F4

Exit the Cleaning screen and turn off the cabinet. The front must be in the closed position to exit.

As well as the F4 function key it is possible to exit the Cleaning screen and turn the cabinet off using the On/Off button closed. (Annex 3 pos. 1). Again, the front must be

2. Main Operation Display. The most relevant data on how to operate the cabinet are shown on a graph. These data are: - Entry speed in the front aperture. Shows in m/s the value of the velocity of the protection barrier for the user. - Temperature inside the cabinet. Shows the temperature in C. - Laminar down flow velocity. Shows the value of the velocity in m/s of the laminar down flow in the work zone. - Clogging of the impulsion filter. Filter clogging is shown in a numerical percentage and also graphically.

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- Clogging of the exhaust filter. Filter clogging is shown in a numerical percentage and also graphically. - Exhaust airflow. The airflow is shown in m3/h. The function buttons acquire the following functions: - F1 - F2 - F3 - F4 Enables/disables the impulsion and exhaust fans. Enables/disables the lighting. Enables/disables the plugs in the work zone. Enables/disables the gas tap in the work zone.

When the fans start up, the system needs a few seconds to stabilise. During this time, it is possible that an alarm may sound because of insufficient flow. To turn the acoustic alarm off, the F4 button changes to fan operation. . This function is only available at the start of

Press the MENU button (Annex 3 pos. 9) to access the Front Positioning display. 3. Front Positioning Display. Enables the user to position the front as he or she likes:

- F1

First press: completely opens the front. Second press: stops the movement of the front.

- F2

First press: moves the front into the work position. Second press: stops the movement of the front.

- F3

First press: completely closes the front and stops the fans. Second press: stops the movement of the front.

- F4

Emergency stop.

Press the MENU button (Annex 3 pos. 9) to access the Utilities display.

4. Utilities display. Provides the user with information on use time of the cabinet, fans, UV lamp and filters, as well as the last time the filters were changed. The function keys provide access to the Maintenance Service and Disinfection display: - F1 - F2 - F3 Provides access to the Maintenance Service display. Provides access to the Disinfection display. Provides access to the Technical Service menu (access restricted to unauthorised staff) via the Password Request display.

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- F4

No function.

Press the MENU button (Annex 3 pos. 9) to access the Password Configuration display. 4.1 Maintenance Service Display. This display shows the date of the last revision and provides the user with access to the following functions: - F1 - F2 - F3 - F4 Records the last revision done. Shows a record of the history of revisions done. Provides maintenance recommendations. Alarms and principle failures.

Press the MENU button (Annex 3 pos. 9) to access the Utilities display. 4.2 Disinfection Display. Access the disinfection menu: - F1 Moves the front to the closed position (Annex 1.2), disconnects the fans and connects the UV light for the time determined by the timer. UV Timer. Put the timer to zero to enable uninterrupted operation of the UV lamp. Provides access to the disinfection with formaldehyde displays which guide the user through the different steps needed for correct disinfection. Exit the Disinfection display and access the Utilities display.

- F2

- F3

- F4

5. Password Configuration Display. Enables the user to configure the options regarding password use: - F1 - F2 Blocks the cabinet to stop use by unauthorised staff. Enables/disables the password request to Connect/Disconnect the cabinet via the Password Request display. Enables the user to change the access password via the Password Request display. If you forget the password, you can use this function to obtain a code. When you give this code to the manufacturers after-sales service, the previous password will be recovered.

- F3

- F4

Press the MENU button (Annex 3 pos. 9) to access the Main Operation display.

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J.

Microprocessor:

The microprocessor maintains total control over the exhaust flow and impulsion flow via the thermoanemometer probe in the exhaust and the thermoanemometer probe in the work zone. Each probe controls the corresponding fan independently (exhaust and laminar down flow) which means double security in the use of the cabinet: The microprocessor consists of: No. 1 2 3 4 5 6 7 8 9 10 11 12 13 Backlit graphic display. Alarms reflected on the microprocessor display: ALARM Excessive laminar down flow (>10%) Insufficient laminar down flow (<-10%) Insufficient exhaust flow (<-10%) Laminar down flow < 0.25 m/s Exhaust flow < 50 m3/h Front open Germicidal lamp enabled Communication failure with redundant channel Glass front blocked by obstacle Blockage in the joint inflation system Joint inflation system does not respond Glass front does not respond Power supply interrupted SHOWN ON THE DISPLAY HIGH LAMINAR DOWN FLOW LOW LAMINAR DOWN FLOW LOW EXHAUST FLOW LAMINAR DOWN FLOW EXHAUST FLOW FRONT OPEN GERMICIDAL CHANNEL 2 COMMUNICATION FRONT BLOCKED JOINT BLOCKED JOINT FAILURE FRONT MOVEMENT POWER SUPPLY FAILURE

K. Auxiliary microprocessor or redundant circuit. If the main microprocessor should fail, the auxiliary microprocessor will activate the optical and acoustic alarms intermittently. If this happens, slide the front to the closed position and cover the cabinet exhaust. Disconnect the equipment and contact the Telstar Technical Service. HIGH SECURITY As well as the visual, optical and acoustic alarms activated by the microprocessor that control the normal operation of the safety cabinet, the three-fan system described in section 2.1. offer the user a cabinet with greater security.

4.6.

DISCONNECTION

To stop the cabinet, it is necessary to push the On/Off button (Annex A3 pos. 1) to access the Password Request display (Section 4.5 point 1). After entering the correct password, the cabinet will disconnect. In the event of the Password Request being disabled (Section 4.5 point 5), the cabinet will disconnect directly after you press the On/Off button.

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4.7.

PROLONGED STOPPAGE

In the event of a prolonged stoppage, try to prevent environmental dust from entering by turning the cabinet off with the front in the closed position (Annex A1.2) and covering up the exhaust outflow at the top of the cabinet (Annex A2 pos. 9). When you turn it on again, it should work like the first time it was turned on. See Sections 3.3 and 4.1.

4.8.

OPTIONAL ACCESSORIES

Support table (Annex A4.1) Active Carbon Filter (Annex A4.2) Non-return exhaust valve (Annex A4.3)

WARNING Gas is a toxic and inflammable substance which in the event of an accident can cause serious damage to the user and the cabinet. The presence of a flame within the cabinet creates turbulence which affects its control and proper functioning, which is why we recommend you do NOT use burners unless strictly essential and if the user is prepared to assume this responsibility. Gas connection should be done in line with the safety standards of each country, region and company. For these reasons, the electrically controlled valve that enables gas entry can only be activated when the fans are working and will automatically disconnect when the alarm indicating a lack of impulsion flow goes off.

5. MANTEINANCE
WARNING All repair and maintenance work in the cabinet should be done by qualified technical staff with knowledge about the maintenance procedures required by the cabinet. Before starting any maintenance work or dismantling the cabinet, the area should be wiped down with formaldehyde (Annex A5.2). Once this has been done, disconnect the cabinet from the mains. In general, a regular control of the proper working of all cabinet components should be done once a year.

5.1.

MAINTENANCE AND CONTROL TABLE

The frequencies determined for each operation, and in particular with regard to changing filters, which appear on the Maintenance Table below are indicative values recommended for normal cabinet service. Very severe work conditions may reduce the time intervals shown on the Table.

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Maintenance Table TEST Impulsin A9.1). filters integrity Test REGULARITY (Annex Minimum once a year or after filter changes

Exhaust filters integrity Test (Anexo A9.2). Minimum once a year or after filter changes Inhalation filter leak Test (only CytoUltra Minimum once a year or after filter changes model) (Annex A9.3). Downflow air velocity Test (Annex A9.4). During cabinet start-up

Set Point alarm downflow air velocity Test Minimum once a year or after filter changes (Anexo A9.4.1). Inflow air velocity Test (Annex A9.5). During cabinet start-up

Set Point alarm inflow air velocity Test Minimum once a year or after filter changes (Annex A9.5.1). Smoke Test (Anexo A9.6) Light Test (Anexo A9.7) Noise level Test (Anexo A9.8) During cabinet start-up During cabinet start-up During cabinet start-up

5.2.

ABSOLUTE FILTERS

The average life of HEPA filters in these cabinets is 4000/5000 hours. After this time, the propelled airflow falls because of the increased loss of load of the filter as it is a mechanical filtering, although this does not mean that filtering efficacy is reduced. When it reaches the minimum flows valued by the probes, the microprocessor activates visual and acoustic alarms. PRECAUTION You cannot replace absolute filters or directly handle fans in MSCs without having first wiped the area down with formaldehyde. Opening the cabinet without taking the right precautions can endanger your health. We recommend it is done by staff especially prepared for this work. When using formaldehyde it is necessary to follow the laws in force in each country. To ensure correct installation and adjustment of the filter, as well as ensuring there are no leaks, filters should be replaced by specialised technical staff equipped with the corresponding control instruments: anemometer, leak detector, aerosol generator, particle counter, etc. Clogged absolute filters left over from changes in safety cabinets should be destroyed. This process is always up the user. Our technical service will never take care of possibly contaminated parts. Optionally, our service offers special packaging for the user to transfer filters for their sterilisation, incineration or destruction.

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5.3.

SPARE PARTS

No. 1. 2. 3. 4. 5. 6. 7.

Ref. 31437 11245 11246 24477 15631 32073 27044

Description Laminar down flow/exhaust fan HEPA Filter 1220 x 610 x 69 HEPA Filter 610 x 610 x 69 Temperature probe Velocity probe 36W germicidal lamp Controller card

6. CLEANING AND DISINFECTION

6.1. SURFACE CLEANING AND DISSINFECTION.

See Annexe A5.1

6.2.

GLASS FRONT CLEANING AND DISINFECTION.

See Annexe A5.2

6.3.

DISINFECTION WITH FORMALDEHYDE

Total decontamination of the cabinet should be done using sterilising gas emission, which ensures penetration in sealed and inaccessible areas of the Cabinet. It also guarantees the sterilisation of the work zone, fan, plenum, HEPA filters and evacuation ducts. Decontamination of the whole of the cabinet, including filters, fan unit and work surfaces, is usually done by fumigation with formaldehyde vapour. Alternatives may be used, e.g., hydrogen peroxide. However, this may not be suitable in certain cases, such as when it is necessary to follow specific regulations. See Annex A5.3 for the decontamination procedure.

7. MAINTENANCE CHART
See Annex A6

8. ELECTRIC DIAGRAMS
See Annexes A7 and A8

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9. TESTS
9.1. IMPULSION FILTERS INTEGRY TEST

See Annex A9.1.

9.2.

EXHAUST FILTERS INTEGRY TEST

See Annex A9.2.

9.3.

INHALATION FILTER LEAK TEST (ONLY CYTOULTRA MODEL)

See Annexe A9.3.

9.4.

DOWNFLOW AIR VELOCITY TEST

See Annex A9.4.

9.4.1.

SET POINT ALARM DOWNFLOW AIR VELOCITY TEST

See Annex A9.4.1.

9.5.

INFLOW AIR VELOCITY TEST

See Annex A9.5.

9.5.1.

SET POINT ALARM INFLOW AIR VELOCITY TEST

See Annex A9.5.1.

9.6.

SMOKE TEST

See Annex A9.6.

9.7.

LIGHT TEST

See Annex A9.7.

9.8.

NOISE LEVEL TEST

See Annex A9.8.

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10.

POSSIBLE PROBLEMS AND SOLUTIONS

No.

Type of error

Causes of error

Correction of error

Exhaust partially covered. Front perforation in the work zone totally/partially covered.

Visually check the exhaust to eliminate any type of obstruction. Visually check the perforation and eliminate any type of obstruction. Increase space between cabinet exhaust and the ceiling. In some cases it is possible to modify or eliminate the false ceiling. Install a support table that is lower. This means the work zone will also have to be lowered. Duct the cabinet (Section 3.5). Check filter clogging with the cabinet in normal operation (Section 4.5 point 2).

Distance between cabinet exhaust and lab ceiling very small. Insufficient exhaust flow 1 Alarm No. 3 (see Section 4.5 point J) Clogged exhaust filter.

Check the date of the last filter change and the hours it has been operating (Section 4.5 point 4). Notify the technical service to change the filter. Check the probe position is correct.

Probe not working properly.

Check that the exhaust box is positioned with the perforation facing upwards. Fit an additional fan at the end of the duct (Section 3.5). Visually check the perforation and eliminate any type of obstruction. Check filter clogging with the cabinet in normal operation (Section 4.5 point 2).

Cabinet ducted and ducting too long. Front and rear perforation in the work zone totally/partially covered. Insufficient laminar down flow 2 Alarm No. 2 (see Section 4.5 point J) Clogged laminar down flow filter.

Check the date of the last filter change and the hours it has been operating (Section 4.5 point 4). Notify the technical service to change the filter.

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Check the probe position is correct (sensor parallel to the flow). Probe not working properly. Check and eliminate any obstruction to the probe. Excessive laminar down flow 3 Alarm No. 1 (see Section 4.5 point J) Air currents in front aperture. Check and eliminate any airflow in front of the cabinet that distorts the reading of the sensor. Check the probe position is correct (sensor parallel to the flow). Probe not working properly. Laminar down flow not working properly Alarm No. 4 (see Section 4.5 point J) Check and eliminate any obstruction to the probe. Check that the connection in the control card is correct (Annex 7 pos. 19). Check the fuse has not melted (Annex A7). Laminar down flow fan stopped. Check that the connection in the control card is correct (Annex 7 pos. 13-14). Notify the Technical Service to change the fan. Exhaust completely covered up. Visually check the exhaust to eliminate any type of obstruction. Check the probe position is correct (sensor parallel to the flow). Exhaust not working properly 5 Alarm No. 5 (see Section 4.5 point J) Exhaust fan stopped. Probe not working properly Check that the connection in the control card is correct (Annex 7 pos. 20). Check that the exhaust box is positioned with the perforation facing upwards. Check the fuse has not melted (Annex A7). Check that the connection in the control card is correct (Annex 7 pos. 15). Notify the Technical Service to change the fan. Front does not move 6 Alarm Nos. 9 and 12 (see Section 4.5 point J) Front is blocked. Check that no obstacle is hindering the movement of the front. After activating Alarm No. 9 it is necessary to press the menu key to disable the alarm and enable the movement of the front again. Check the probe position is correct (sensor parallel to the flow).

Probe not working properly.

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Fuse is melted. Front motor failure. Fastening cables out of position. Luminaire not working properly 7 Luminaire does not turn on Fluorescent lights not working Ballast is damaged Front not in closed position UV germicidal lamp does not turn on

Replace fuse (Annex A7). Notify the Technical Service to change the motor. Call the Technical Service to receive instructions on how to adjust the cable. Check that the connection in the control card is correct (Annex 7 pos. 9). Slightly turn the fluorescent light to ensure good contact. Change the fluorescent bulb. Change the ballast. Check the front is working. Check the lamp cap is connected properly.

UV lamp does not work

Check that the connection in the control card is correct (Annex 7 pos. 11). Change the UV lamp.

Ballast is damaged Intermittent alarm activated 9 Alarm No. 8 (see Section 4.5 point J)

Change the ballast. Raise the front and check the security micro is not loose. Call the Technical Service to receive instructions on adjusting micros. Call a specialised technician to replace the control.

Security micro in the front position activated

Main control is damaged

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11.

CERTIFICATES

11.1. CE
See Annex A10.1.

11.2. GUARANTEE
See Annex A10.2.