Conclusion

PCR (polymerase chain reaction) is a technique of amplifying a single copy of DNA. Thousands and millions of copies of DNA can be produced through this technique having particular sequences. One the other hand, Agarose gel electrophoresis is a technique used to analyze DNA molecules and

have a great role in many fields. AGE may be used to scatter a varied population of DNA and RNA fragments by length as well as to approximate the size of DNA and RNA fragments or separate proteins according to its charge. In the result of this activity the DNA molecules in each samples moved towards the positive charged area in which smaller fragments move faster and travel farther compared to larger fragments from a negatively charged point of the electrophoresis box. There are DNAs consisting of homozygous dominant and these are not cut by restriction enzymes leading to uncut fragments of DNA and will stay together creating a single band on the gel which was observable on DNA samples of ASU and Kinandang patong. The other type contains homozygous for the recessive allele in which DNA split by the enzyme leading into fragments of two diverse sizes making two bands on the gel after electrophoresis.

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APPENDIX

Table 1. PCR cocktail composition PCR cocktail PCR mix Forward primer Reverse primer DNA template Nuclease Free water volume 5uL 1uL 1uL 2uL 1uL x 11 55uL 11uL 11uL 22uL 11uL x2 110uL 22uL 22uL 44uL 22uL

Table 2. The DNA samples that appeared visible in the result DNA SAMPLE WISH 121 (Basmati rice) CATEGORY Homozygous for the dominant allele

WISH 131 (ASU)

Homozygous for recessive allele

WISH 134 (Kinandang patong)

Homozygous for recessive allele