DOUGLAS B.

HEIMARK
Charlottesville, VA 22903 804-212-9124 dheimark@icloud.com

ANALYTICAL CHEMIST
Versatile, highly skilled analytical chemist with 30+ years of experience performing pharmacological and biochemical research within demanding laboratory environments. Exceptional eye for detail; strong track record of leveraging critical thinking skills to resolve problems requiring in-depth knowledge of scientific techniques and methods. Repairs and maintains laboratory equipment and inventory to optimize workplace productivity and efficiency and reduce operating costs. Excels both independently and in team-oriented work environments. Areas of Expertise: Product / Sample Testing Inventory Control Records / Document Maintenance Biochemistry Pharmacology Project Management Laboratory Management Research / Analysis Cross-Functional Collaboration Equipment Maintenance Efficiency / Productivity

PROFESSIONAL EXPERIENCE
ALLOMED LLC, Charlottesville, VA Senior Consultant, 2006 Present Manage all daily aspects of the laboratory to cultivate an efficient, productive workplace, including inventory management, reagent solution preparation, and data collection/recording. Prepare manuscripts, symposium posters, and grant applications for submittal. Perform column/TLC chromatography and protein/carbohydrate analysis to analyze and comprehend biochemical, enzymatic, and genetic mechanisms of insulin resistance and Type 2 diabetes to assist in the development of effective pharmaceuticals. Troubleshoot analytical instruments to ensure proper functionality and operation. INSMED, Inc., Glen Allen, VA Research Associate, 2000 2006 Carried out routine maintenance and repair of HPLC instruments. Instrumental in chromatographic isolation and analysis of insulin second messenger by LC/MS, GC/MS, TLC and ion chromatography; subsequent biologic activity was followed enzymatically. UNIVERSITY OF VIRGINIA DEPARTMENT OF PHARMACOLOGY, Charlottesville, VA Laboratory Specialist C, 1984 2000 Provided crucial support in isolating natural compounds associated with Type 2 diabetes by utilizing multiple chromatographic techniques. Facilitated enzymatic analysis and detection by GC/MS and ion chromatography. Supervised students/junior employees in second messenger isolation, chromatography, and various analyses. Additional position as Laboratory Specialist A at University of Virginia (1980 1984).

EDUCATION & TRAINING

Bachelor of Science Medical Technology, Temple University, Philadelphia, PA Bachelor of Arts Chemistry, Windham College, Putney, VT GC/MS Theory, Operation, Maintenance, and Repair, Varian Company, Blue Ash, OH HPLC Theory, Operation, Maintenance, and Repair, Waters, Boston, MA

TECHNICAL PROFICIENCY
HPLC, GC/MS, Electrophoresis, Column Chromatography, TLC Instrument Repair/Maintenance Microsoft Word/PowerPoint/Excel, GraphPad Prism, Endnote

DOUGLAS B. HEIMARK
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RESEARCH & PUBLICATIONS

Sellner KG, Lyons L. Perry ES, Heimark DB. Assessing Physiological Stress in Thallasiosira Fluviatilis (Bacillariophyta) and Dunaliella Teriolecta (Chlorophyta) with DCMU-enhanced Fluorescence. Journal of Phycology 1982;18(1):142-8. Larner J, Romero G, Kennington A, et al. Duality in the mechanism of action of insulin. Adv Second Messenger Phosphoprotein Res 1990;24:290-4. McCoy J, Mann B, Vedvick T, Pak Y, Heimark D, Petri WJ. Structural analysis of the light subunit of the Entamoeba histolytica galactose-specific adherence lectin. J Biol Chem 1993;268(32):24223-31. Huang L, Heimark D, Linko J, Nolan R, Larner J. A model phosphatase 2C --> phosphatase 1 activation cascade via dual control of inhibitor-1 (INH-1) and DARPP-32 dephosphorylation by two inositol glycan putative insulin mediators from beef liver. Biochem Biophys Res Commun 1999;255(1):150-6. doi: 10.1006/bbrc.1999.0111. Sleight S, Wilson B, Heimark D, Larner J. G(q/11) is involved in insulin-stimulated inositol phosphoglycan putative mediator generation in rat liver membranes: co-localization of G(q/11) with the insulin receptor in membrane vesicles. Biochem Biophys Res Commun 2002;295(2):561-9. Sun T, Heimark D, Nguygen T, Nadler J, Larner J. Both myo-inositol to chiro-inositol epimerase activities and chiroinositol to myo-inositol ratios are decreased in tissues of GK type 2 diabetic rats compared to Wistar controls. Biochem Biophys Res Commun 2002;293(3):1092-8. doi: 10.1016/S0006-291X(02)00313-3. Larner J, Price J, Heimark D, et al. Isolation, structure, synthesis, and bioactivity of a novel putative insulin mediator. A galactosamine chiro-inositol pseudo-disaccharide Mn2+ chelate with insulin-like activity. J Med Chem 2003;46(15):3283-91. doi: 10.1021/jm030071j. Brautigan D, Brown M, Grindrod S, et al. Allosteric activation of protein phosphatase 2C by D-chiro-inositolgalactosamine, a putative mediator mimetic of insulin action. Biochemistry 2005;44(33):11067-73. doi: 10.1021/bi0508845. Nascimento N, Lessa L, Kerntopf M, et al. Inositols prevent and reverse endothelial dysfunction in diabetic rat and rabbit vasculature metabolically and by scavenging superoxide. Proc Natl Acad Sci U S A 2006;103(1):218-23. doi: 10.1073/pnas.0509779103. Hans SK, Camara F, Altiti A, et al. Synthesis of C-glycoside analogues of -galactosamine-(1-->4)-3-O-methyl-Dchiro-inositol and assay as activator of protein phosphatases PDHP and PP2C. Bioorg Med Chem 2010;18(3):1103-10. Gluck G, Anguelova T, Heimark D, Larner J. Synergistic Effects of D-Chiro-Inositol and Manganese on Blood Glucose and Body Weight of Streptozotocin-Induced Diabetic Rats. Current Bioactive Compounds 2010;6:90-6. Farias VX, Macdo FHP, Oquendo MB, et al. Chronic treatment with D-chiro-inositol prevents autonomic and somatic neuropathy in STZ-induced diabetic mice. Diabetes, Obesity and Metabolism 2011;13(3):243-50. Heimark D, McAllister J, Larner J. Decreased myo-inositol to chiro-inositol (M/C) ratios and increased M/C epimerase activity in PCOS theca cells demonstrate increased insulin sensitivity compared to controls. Endocr J. 2014;61(2):111-17.