512874AC

TM

DU Spectrophotometers Installation Instructions

Single Cell Holders
P/N 594439 P/N 523407 P/N 523403 P/N 523382 P/N 517038 P/N 517037 P/N 517151 Single Cell Holder Single Cell Holder, Water Single Cell Holder, Peltier 50L Single Microcell Holder Cylindrical Cell Holder Rectangular Cell Holder Turbidity Cell Holder

Beckman Coulter, Inc. 250 S. Kraemer Blvd., Brea, CA 92821 Copyright 2009 Beckman Coulter, Inc.

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Table of Contents
General Installation..........................................................................1 Cell Holder Types ............................................................................3 Single Cell Holder .................................................................... 3 Single Cell Holder, Water......................................................... 4 Single Cell Holder, Peltier ........................................................ 5 50 L Single Cell Holder.......................................................... 6 Rectangular Cell Holder ........................................................... 7 Cylindrical Cell Holder............................................................. 8 Turbidity Cell Holder................................................................ 9 Conditioning ........................................................................... 15 Filling...................................................................................... 16

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General Installation
The Single Cell Holders have been designed to be installed and replaced quickly and easily with two large thumb screws and to be used on a Static Mount (included with DU Series 7000, 600 and 800 Systems) or an optional Transport. The various Single Cell Holders are described later. NOTICE The installation of a cell holder requires the prior installation of the Static Mount, P/N 517023, the Standard Transport, P/N 517522 (optional), or the High Performance Transport, P/N 517040 (optional). WARNING Turn off and unplug power before installing accessories. Tools required: None (may need a flatblade screwdriver to remove previously installed cell holder). 1. Remove and store any other cell holder that is installed. 2. Position the appropriate cell holder on the Static Mount or the Transport and secure by tightening the two thumb screws. If the cell holder has a FRONT label, seat the cell holder, such that the label is towards the front of the sample compartment. The figures below show a Single Cell Holder installed on the Static Mount (Figure 1) and on the Transport Accessory (Figure 2) in a DU Series 7000 Spectrophotometer.

Thumb Screws

Front Label
Figure 1.

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Thumb Screws

Front Label

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Figure 2. 3. Refer to the DU Series 7000, 600 or 800 Spectrophotometer Operating Instructions for information on the operation of the instrument. NOTICE When the Single Cell Holder is mounted on the Transport, the Transport must be positioned correctly. To guarantee proper positioning, select Single Cell (DU Series 600 & 7000) in the Device Control Window and select Single Cell Holder (DU Series 800) in the Transport/Holder Tab of the Accessories Window.

Cell Holder Types

Single Cell Holder The unheated Single Cell Holder has been designed to accommodate multi-purpose standard 10 x 10 mm cuvettes. The horizontal position is adjustable with a screw in the bottom of the cell holder. This is preset at the factory. To reset the position, if the screw was moved, or to optimize the position, refer to Appendix A. NOTICE When securing a cell in the cell holder, do not over-tighten the nylon screws.

Use with Sipper Flow Cell (P/N 599925) You may want to secure the flow cell by tightening the two nylon screws and adjust the height to fit the beam width to the flow cell window. Refer to Appendix A. Use with Ultra-Microcell (P/N 514261) Use the 5/32" Allen wrench to remove or lower the screw at the bottom of the cell holder so it does not extend into the cell holder. Secure the Ultra-Microcell in the cell holder by gently tightening the two nylon screws. To optimize the horizontal position, refer to Appendix B for the Static Mount and Appendix C for the Transport Mount.

Single Cell Holder, Water The water temperature-controlled Single Cell Holder has been designed as an inexpensive solution to temperature control for standard 10 x 10 mm cuvettes. 1. Connect the water tubing to the water ports installed on the front of the sample compartment (DU Series 600 and 800) or the Transport Cover (DU Series 7000). Figure 3 shows the cell holder installed on the Transport in a DU Series 800 Spectrophotometer.

Water Ports

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Figure 3. NOTICE The water tubing must be attached to the water ports, even if the accessory is not being water regulated. If the water tubing is not attached, it may interfere with sample measurements. As an alternative, the water tubing can be removed from the accessory if water regulation will not be used. 2. Follow the instructions in Appendix D for information about providing water for temperature regulation.

Single Cell Holder, Peltier The Peltier temperature-controlled Single Cell Holder requires the Peltier Temperature Controller (Standard, P/N 517057 (120V) and P/N 517058 (240V), or High Performance, P/N 517450 (120V) and P/N 517451 (240V)) and the High Performance Transport (P/N 517040) to remove excess heat. The holder has been designed for highly accurate temperature control for standard 10 x 10 mm cuvettes. 1. Plug the temperature cable into the connector on the left-hand sample compartment wall. Figure 4 shows the cell holder installed on the Transport and connected to a DU Series 800 Spectrophotometer.

Temperature Cable

Connector

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Figure 4. 2. Install the Peltier Temperature Controller as instructed in the Installation Instructions, P/N 512872.

50 L Single Cell Holder The 50 L Single Cell Holder only accepts 50 L Microcells. Follow the instructions in Appendix E to condition and fill the microcells correctly. Figure 5 shows the cell holder installed on the Transport in a DU Series 800 Spectrophotometer.

Microcell

Cell Holder
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Figure 5.

Rectangular Cell Holder The Rectangular Cell Holder has been designed to accommodate rectangular cuvettes with large path lengths (10 mm wide and up to 50 mm long). To adjust the cell holder to the cuvette, loosen the Phillips screw, slide the left-hand side to the required position, and then tighten the screw. When the cell holder is adjusted properly, the cuvette should be held tightly, yet easily inserted and removed. Figure 6 shows the cell holder installed on the Transport in a DU Series 800 Spectrophotometer.

Adjustment Screw

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Figure 6.

Cylindrical Cell Holder The Cylindrical Cell Holder has been designed to accommodate cylindrical cuvettes with large path lengths (up to 100 mm long). Figure 7 shows the cell holder installed on the Transport in a DU Series 800 Spectrophotometer.

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Figure 7.

Turbidity Cell Holder The Turbidity Cell Holder has been designed to give accurate quantitative measurements of light scatter in DU Series 600 and 800 Spectrophotometers with conventional forward-optics design. This cell holder should be used whenever bacterial cultures or other turbid samples are to be quantitated by absorbance measurements. For more information, please refer to our Product Information Bulletin T-1795 Turbidity Cell Holder for DU Series 600 UV/Vis Spectrophotometers. Figure 8 shows the cell holder installed on the Transport in a DU Series 800 Spectrophotometer.

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Figure 8. NOTICE Components of the Turbidity Cell Holder have been factory aligned. Do not disturb the screw which maintains this alignment.

Appendix A Optimizing the Height Adjustment for the Single Cell Holder
The unheated Single Cell Holder has a screw in the bottom which is used to adjust the height of the cuvette to the optimum position. The screw is factory set in the optimum position for use with the Sipper Flow Cell, P/N 599925. For the Ultra-Microcell, P/N 514261, the screw should be removed or lowered so that it is flush with the bottom of the cell holder. Tools required: Allen wrench, 5/32" (provided with the cell holder). To find the optimum position for the Sipper Flow Cell: 1. 2. 3. 4. 5. Verify that the Sipper Accessory is installed using the directions in the Installation Instructions, P/N 512873. Fill the flowcell with distilled water, using the {FLUSH} button on the Sipper Pump. Set the instrument to take transmittance readings at 500 nm. Blank the instrument, setting the distilled water equal to 100%T. Loosen the two nylon screws and remove the flow cell from the cell holder. Insert the Allen wrench into the screw and turn 1/4 turn in a clockwise direction. Reinsert the flow cell into the cell holder and secure by gently tightening the two nylon screws. Take a reading. a. b. If the reading is greater than 100%T, repeat the instructions in steps 5 and 6, until the maximum reading is obtained. If the reading is less than 100%T, repeat the instructions in steps 5 and 6, turning the screw in the counter-clockwise direction, until the maximum reading is obtained. NOTICE If the reading exceeds 150%T at any time during the above procedure, re-blank to reset the reading to 100%T. Then continue until the maximum reading is obtained.

6. 7.

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Appendix B Optimizing the Horizontal Adjustment for the Single Cell Holder (Static Mount and Transport Mount with Sipper Flowcell and Static Mount with Ultra-Microcell)
When the instrument is configured for use with a Single Cell Holder on the transport, the transport position is set to 57 mm. Tools required: Phillips screwdriver. To find the optimum position: 1. Verify that the Sipper Accessory is installed using the directions in the Installation Instructions, P/N 512873. For use with the Ultra-Microcell, follow the instructions in steps 1 and 2 of Appendix C, then continue with step 3 of these instructions. Fill the flowcell with distilled water, using the {FLUSH} button on the Sipper Pump. Set the instrument to take transmittance readings at 350 nm. Blank the instrument, setting the distilled water equal to 100%T. Slightly loosen the Phillips screw on the top of the cell holder. Slide the top of the cell holder toward the back of the sample compartment a small amount. Then take a reading. a. If the reading is greater than 100%T, continue sliding the top of the cell holder toward the back of the sample compartment and take additional readings. Repeat until the maximum reading is obtained. If the reading is less than 100%T, slide the top of the cell holder toward the front of the sample compartment and take additional readings. Repeat until the maximum reading is obtained. NOTICE If the reading exceeds 150%T at any time during the above procedure, re-blank to reset the reading to 100%T. Then continue until the maximum reading is obtained. NOTICE In DU Series Spectrophotometers, the peaking tool (provided for visible source peaking) can be inserted into the slot on the top of the cell holder. Turning the peaking tool will move the top of the cell holder. 11

2. 3. 4. 5. 6.

b.

Appendix C Optimizing the Horizontal Adjustment for the Single Cell Holder (Transport Mount with Ultra-Microcell)
When the instrument is configured for use with a Single Cell Holder on the Transport, the Transport position is set to 57 mm. The optimum position may be a few tenths of a millimeter on either side of 57 mm. To find the optimum position: 1. 2. 3. Fill a capillary with distilled water using the directions in the Installation Instructions provided with the Ultra-Microcell. Insert the capillary into the Ultra-Microcell. DU Series 600 and 7000 Spectrophotometers, only. Click on DEVICES, located in the Permanent Menu Bar at the bottom of the display. The Device Control Window will be displayed. Verify that the cell holder is positioned in the Single Cell position. DU Series 800 Spectrophotometers, only. Click on the Accessories menu item, located in the System Menu. The Accessories Window will be displayed. Under the Transport/ Holder Tab, select Single Cell Holder. 4. 5. Set up the RediRead Window to take transmittance readings at 500 nm. Blank on the Ultra-Microcell. DU Series 600 and 7000 Spectrophotometers, only. Use the Device Control Window to position the Transport at 56 mm. Then [EXIT] the window and take a transmittance reading in the RediRead Window. DU Series 800 Spectrophotometers, only. Use the Accessories Window to position the Transport at 56 mm. Select [OK] to exit the window and take a transmittance reading in the RediRead Window. 6. 7. Repeat step 5, taking readings every 0.1 mm from 56 to 58 mm. Find the millimeter location with the maximum transmittance reading and input it into the Device Control Window (DU Series 600 and 7000) or into the Accessories Window (DU Series 800) as the Transport position.

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Appendix D Water Tubing and Connection

The water-controlled Single Cell Holders are supplied with the parts shown in Figure 9, which are used to connect the cell holder to the water supply.

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Figure 9. Water tubing and parts: 1. 2. 3. 4. Tubing, P/N 523300, 5 meters Tubing assembly with restrictor, P/N 539012 Hose Clamps, P/N 884596, 2 each Quick Disconnect Fitting, P/N 514369 *

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*The Quick Disconnect Fittings are used on DU Series 7000 Spectrophotometers only.

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Locate the water port assembly. It should be installed on the left-hand side of the Transport (DU Series 7000) or on the front of the sample compartment (DU Series 600 and 800). Attach the tubing provided to the outside of the water ports and secure with hose clamps. Figure 10 shows the water tubing connection on a DU Series 7000 Spectrophotometer.

Hose Clamps

Water Ports

Water Tubing
Figure 10. NOTICE Located near the end of one of the tubes is a white flow restrictor. This piece of tubing should be attached to the water supply, with the restrictor closer to the water supply than to the instrument.

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2. 3.

Connect the tubing with the flow restrictor to the water supply. The tubing without the restrictor is the water outlet. DU Series 7000 Spectrophotometers, only. When water control is not being used, the water ports with the tubing attached can be disconnected from the instrument using the thumb screw.

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Appendix E Conditioning and Filling of 50 L Microcells

The following describes the treatment of 50 L microcells. To obtain accurate results, be certain to use the appropriate microcell holder. Conditioning Use the following procedure to wet the surface of each 50 L microcell before using it, whenever it has been allowed to completely dry. 1. Fill a micro pipette with about 75 L of methanol. Holding the microcell on the frosted side, place the tip of the micro pipette on the bottom of the microcell. Fill and empty the microcell several times with methanol. See Figure 11.

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Figure 11. NOTICE If using a micro pipette with a glass tip, be careful not to scratch the inside of the microcell.

2. 3.

Using the micro pipette, remove the methanol from the microcell and discard the methanol. Repeat steps 1 and 2 using water, then repeat steps 1 and 2 using the solvent or buffer solution that the sample is prepared in. The microcell is now ready to use. Repeat the above procedure for each microcell. 15

Filling 1. Fill a micro pipette with at least 50 L of solution. Verify that there are no bubbles in the pipette tip. NOTICE If using a micro pipette with a glass tip, be careful not to scratch the inside of the microcell.

2.

Holding the microcell on the frosted side, place the tip of the micro pipette on the bottom of the microcell. Fill the microcell with solution. See Figure 11 above. NOTICE Before any sample readings are taken, the instrument must be blanked with the microcell in the cell holder, filled with blank solution (solvent or buffer).

3.

Tap the top of the microcell with a finger nail to distribute the solution and remove air bubbles. As an alternative, gently tap the microcell on the lab bench or another solid object. Wipe the window of the microcell with lens paper or a lint free tissue. Place the microcell in the cell holder, such that the red dot is toward the back of the sample compartment. See Figure 12. NOTICE The red dot on the microcell allows the user to place the cell in the same orientation when filled with subsequent samples.

4. 5.

6.

Verify that the microcell is seated firmly in the cell holder by pressing down on it with a pipette tip, spatula, finger nail, etc. Because of the small volume, if the microcell is not seated firmly, inaccurate readings may occur.

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View the microcells in the cell holder through the aperture to verify that clear solution is visible for all the microcells. A meniscus or bubbles should not be seen. See Figure 12.

Red Dot

Microcell

Aperture
Figure 12.

Front Label
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Appendix F Emptying, Cleaning and Refilling of Microcells

1. 2. 3. Remove the microcell from the accessory, holding it on the frosted sides. Remove the solution from the microcell using a micro pipette. The tip of the micro pipette can be used to remove most of the sample. If desired, rinse the microcell with solvent or buffer solution before filling with the next sample. As an alternative, the microcell can be rinsed with distilled water and air or vacuum dried. Do not use a laboratory air source or an abrasive cloth to dry the microcell. NOTICE Proper cuvette cleaning is very important and will increase their useful life and provide more consistent results. Never use highly alkaline solutions, abrasive materials, or hot concentrated acids to clean the cells. At the end of the day, clean your cuvettes thoroughly, dry them completely, and store them in a padded cuvette box or other suitable container. 4. To clean the microcell, rinse with a mild solvent (distilled water for aqueous solutions, an organic solvent for organic solutions) as soon as possible after use. This should be followed by several rinses with a dilute alkaline solution using a micro pipette. Rinse the cell immediately with distilled water, air dry and store. To remove protein deposits from the microcell, soak in a dilute alkaline solution at 56 C for 15 minutes. Rinse the cell immediately with distilled water, air dry and store. For other hard-to-remove deposits, wash with a solution made from equal parts by volume ethanol and 3N HCl. Rinse the cell immediately with distilled water, air dry and store. Do not soak the cell in this solution for more than 30 seconds. If the cell cannot be cleaned immediately, soak the cell in distilled water. Do not soak the cell in a detergent solution for more than 15 minutes, as it can etch the cell windows.

5.

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Notes

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