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B.TECH. BIOTECHNOLOGY
SCHOOL OF BIOENGINEERING AND FOOD TECHNOLOGY SHOOLINI UNIVERSITY, BAJHOL (H.P.) 173229
Certificate
It is certified that LAVANYAM PATHANIAand LALIT DADWAL Students 2045 & 2043
for the session 2010-2014, have completed the project report (Bt-212) entitled BIOPROCESS under our supervision. The project report is, in my opinion, worthy for consideration for the award of credits of the subject Seminar and Term Paper (BT-212) in accordance with the rules and regulations of School of Bioengineering and Food Technology, Shoolini University. I wish them all success in their future endeavors.
Screening can never be considered a routine activity. In order to be successful, screening must be an interdisciplinary activity. There are no universal screening methods
Chemotherapeutically useful products for the following areas: activity against antibioticresistant strains, tumors, fungi and viruses Enzyme inhibitors and pharmacologically active substances (hormones, etc.) Better starter cultures for the food industry Microorganisms that are capable of degrading hazardous and persistent chemicals
Strategies for the isolation of a suitable industrial microorganism from the environment:
Shotgun approach. Samples of free living microorganisms, biofilms or other microbial communities are collected from animal and plant material, soil, sewage, water and waste streams, and particularly from unusual man-made and natural habitats. These isolates are then screened for desirable traits.
Objective approach. Take samples from specific sites where organisms with the desired characteristics are considered to be likely components of the natural microflora.
Enrichment of special types of microorganisms / appropriate selective media and growth condition.
General scheme of isolation of strains Samples suspended in sterile water containing Tween. The sample is vigorously agitated The supernatant is diluted 10-1-10-10
Diluted samples are plated on various culture media and then incubated. (choosing or developing the appropriate selective media and growth conditions) Picked single colonies are re-streaking
Provide a method to isolate microorganisms from mixed cultures with improved properties. e.g. thermophilic or alcohol tolerant strains
isolated and characterized from these strains. These antibiotics turned out to be almost all either acetylmalonyl or amino acid derivatives.
Inhibited Bacillus subtilis but did not inhibit Acholeplasma laidlawii (lacks a cell wall) Inhibited the incorporation of meso-diaminopimelic acid but did not inhibit the incorporation of leucine Eliminated substances whose molecular weights were greater than 1,000.
Azureomycin was discovered from culture filtrates of 10,000 strains (fungi, bacteria and actinomycetes).
Finding NAD(H)-dependent dehydrogenases for the reduction of prochiral 2ketoacids to the corresponding chiral 2-hydroxyacids:
Lactic acid bacteria Cultivated anaerobically Centrifugation, disintegration, high-speed centrifugation, filtration ((0.2mm)
Crude extract to fast protein liquid chromatography (FPLC) (Instrumental aids) Enzyme assay: 2-ketoacids, NADH, buffer, decrease in A340.