CPB 30103: Biochemical Engineering By Law Jeng Yih

Tutorial 1 Section A 1. a. b. c. d. e. 2. a. b. c. d. e. f. Biomolecules: behave differently than molecules outside of a living system are formed from a backbone of hydrogen are formed primarily from ionic bonds are of four basic types a and d are correct Carbohydrates: are formed from C, H, O, and N would leave a carbon atom if atoms forming water were removed form readily accessible storage in the form of starch and glycogen include sugars c and d are correct b, c, and d are correct

3. In the Fischer projection of D-glycealdehyde, the OH on the chiral carbon is drawn a. to the left b. below c. above d. to the right 4. a. b. c. d. 5. a. b. c. d. In L-glucose, the OH on carbon 5 is drawn to the left below above to the right The disaccharide sucrose is composed of the monosaccharides D-glucose + D-glucose D-fructose + D-galactose D-glucose + D-fructose D-glucose + D-galactose

CPB 30103: Biochemical Engineering By Law Jeng Yih

6. What is the stereochemical relationship between D-erythrose and Lerythrose? a. they are epimers b. they are diastereoisomers c. they are anomers d. they are enantiomers 7. a. b. c. d. e. f. Nucleic acids: include organic bases include phosphorylated sugars are found in the nucleus contain the information structure of the cell all are correct a, c, and d are correct

8. Given the DNA sequence below, what is the complementary DNA sequence? 5'-AATTGGCC-3' a. 5'-AAUUGGCC-3' b. 5'-AATTGGCC-3' c. 5'-UUAACCGG-3' d. 5'-GGCCAATT-3' e. 5'-GGCCAAUU-3' 9. The picture shows an x-ray diffraction of DNA. The x-ray diffraction of DNA led to the idea that DNA

a. b. c. d.

is a double helix contains paired bases can copy itself is a very long molecule

CPB 30103: Biochemical Engineering By Law Jeng Yih

Section B 1. a) b) c) Define the following terms: biochemical engineering isomers enantiomers

2. What are the 3 major energy sources for a cell? 3. a) b) c) Identify the monosaccharides in each of the following: lactose maltose sucrose

4. Write the cyclic form of alpha-D-galactose.

5. Write the Fisher projection of D-glucose. 6. What are the possible tripeptides formed from one each of leucine, glycine, and alanine? 7. a) b) c) d) Identify the level of protein structure: Beta pleated sheet Order of amino acids in a protein A protein with two or more polypeptide chains Disulfide bonds between R groups

8. What are the products of the complete hydrolysis of Ala-Ser-Val? 9. List out 5 examples of protein and its function.

CPB 30103: Biochemical Engineering By Law Jeng Yih

10. Classify the following structure as that of a nucleoside or a nucleotide. Identify its components (sugar? base? phosphate?). Is this a purine or a pyrimidine?

11. Summarize the differences between DNA and RNA. 12. Diagram and explain the Central Dogma of Molecular Genetics. 13. What is the pH of a buffer mixture composed of 0.12 M lactic acid ( HC3H5O3 where Ka = 1.4 x 10-4 ) and 0.10 M sodium lactate ( NaC3H5O3 ). 14. A 1 L solution containing 0.100 moles of acetic acid, HC2H3O2 and 0.100 moles of C2H3O2- forms a buffer solution, pH = 4.74. What is the pH after 0.020 moles of NaOH are added? Ka of acetic acid = 1.8 x 10-5.