Jacob, Jeric T.

IV- BSBT

Semi Detailed Lesson Plan Prof. Belmi

Structure of Endoplasmic Reticulum I. Targets Given the necessary material, students should be able to :

1.)differentiate smooth and rought endoplasmic reticulum; 2.)show the structure of endoplasmic reticulum; and 3.)recognize the imporatance of endoplasmic reticulum as part of the cell II. Subject Matter

1.)Structure of Endoplasmic reticulum A.)Smooth Endoplasmic reticulum B.) Rough Endoplasmic Reticulum

III.Preliminary Activities A.) Prayer Student lead a prayer B.)Greetings Teacher will greet the students

C.)Motivation There are cut out pictures of endoplasmic reticulum. Recreate it to form again

IV. Lesson Proper Endoplasmic Reticulum It is a canal like membrane system that extends into cytoplasm

Cisternal Space

The endoplasmic reticulum is a network of tubules and flattened sacs that serve a variety of functions in the cell. The ER is very extensive extending from the cell membrane through the cytoplasm and forming a continuous connection with the nuclear envelope. It has large amount of fold called endoplamic reticulum lumen or cisternal space

A space containing fluid, such as those occurring between the membranes of flattened sacs of the Golgi apparatus and the endoplasmic reticulum, also between the two membranes of the nuclear envelope.

ER Membrane Production of all transmembrane proteins and lipids for most of the cell organelles including endomembrane system Production of lipids in mitochondrial and peroxisomal

Two Proteins In Endoplamic reticulum 1.)Transmembtane Proteins Partly translocated across ER membrane Embedded the ER

2.) Water Soluble Protein Fully tanslocated across the ER membrane and release in ER lumen It functions through signal

Rough Endoplasmic reticulum A part of endoplasmic reticulum which is for performing the synthesis of proteins and transport of many biochemical compounds in a cell. It is made up of a system of tubules, vesicles, and cisternae and on the surface it has ribosomes which make it appear rough hence its name. Another function of rough endoplasmic reticulum is for providing an internal structural skeleton to support the cell's shape, as storage of the synthesised materials and minerals, such as calcium in myocytes, forming an internal network through which materials can be transported

Two parts of Ribosomes in RER 1.)Membrane bound ribosomes A membrane bounded ribosmes which synthesized on the rough ER membrane and remain membrane associated as they move to their final destinations along the same pathway followed by continuously secreted proteins.

Synthesized all proteins that is being translocated into ER

2.) Free Ribosomes These are the unttached to any membrane, or a ribosome which is float on the cytoplasm of the cell

Ribosome A ribosome is usually found in eukaryotic cells are tiny, round organelles that generate proteins from mRNA. Ribosomes are composed of protein and ribosomal RNA. Ribosomes are produced in the nucleolus of the cell. The proteins that make up the ribosome are brought into the nucleolus and assembled into subunits. Afterward, the subunits are transported to the cytoplasm of the cell. The subunits of a ribosome join when it beings to build proteins.

Location of Free and Bound Ribosomes The place where the ribosome is located is one of the differences between free and bound ribosomes. Free ribosomes are located in the cytoplasm of the cell. They are not attached to any structure, but they may group together with other ribosomes to form polysomes. In the cytoplasm, ribosomes are free floating. They can move all around the cell. Bound ribosomes are located on the surface of the endoplasmic reticulum. The endoplasmic reticulum that contains ribosomes is described as the rough endoplasmic reticulum because of the bumpy surface. Bound ribosomes can not move to other areas of the cell. They are attached to the cytosolic side of the endoplasmic reticulum. Smooth Endoplasmic Reticulum Is involved in the synthesis of carbohydrate, female hormones and lipids. These organelles also help to detoxify certain compounds in liver cells. smooth ER also aid in the contraction of muscle cells. Also called as transitional ER because it contain ER exit site from which transparort vesilce carrying newly synthesized protein and lipids bud off fro transport in golgi apparatus Expanded SER is for accomodating enzyme Example is liver Two types of Vesicle in ER 1.)Secretory Vesicle A secretory pathway, proteins travel from the ER through the Golgi apparatus to arrive at the cell surface or at vacuoles. This biosynthetic, or anterograde, traffic is balanced by retrograde traffic running in the opposite direction. The retrograde traffic allows for endocytosis of extracellular molecules as well as recycling of membranes and proteins to maintain the integrity of the different compartments. 2.)Transport Vesicle

It carries a protein and receive a signal from the cell sender Transport vesicles select cargo molecules and move them to the next compartment in the pathway, while others retrieve escaped proteins and return them to a previous compartment where they normally function. Thus, the pathway from the ER to the cell surface involves many sorting steps, which continually select membrane and soluble lumenal proteins for packaging and transport a vesicles or organelle fragments that bud from the ER and Golgi apparatus.

Nuclear Envelope The nuclear envelope separates the contents of the nucleus from the cytoplasm and provides the structural framework of the nucleus. The nuclear membranes, acting as barriers that prevent the free passage of molecules between the nucleus and the cytoplasm, maintain the nucleus as a distinct biochemical compartment. The outer nuclear membrane is continuous with the endoplasmic reticulum, so the space between the inner and outer nuclear membranes is directly connected with the lumen of the endoplasmic reticulum.