Macasiano, Debbie B.

Sacarez, Daisy

Lethal Genes 1. A man is said to have a Huntingtons Disease. If he will be mated with a woman with Huntingtons Disease, what is the ratio of their offspring that will live and die? Hh x Hh PR: 0:1

1HH:2Hh:1hh

1 offspring will die at birth, 2 will live with Huntingtons Disease but willdie after sexual maturity & 1 will live with no Huntingtons Disease.

Linkage & Recombination 1. A kidney-bean-shaped eye is produced by a recessive gene k on the third chromosome of Drosophilia. Orange eye color, called "cardinal," is produced by the recessive gene cd on the same chromosome. Between these two loci is a third locus with a recessive allele e that produces ebony body color. Homozygous "kidney," cardinal females are mated to homozygous ebony males. The trihybrid F 1 females are then testcrossed to produce the F2. Among 4000 F2 progeny are the following:

(a) Determine the linkage relationships. (b) Estimate the map distances.

Answer: a) The parents are homozygous lines:

The linkage relationships in the trihybrid F1 can also be determined directly from the F2. By far the most frequent F2 phenotypes are kidney, cardinal (1761) and ebony (1773), indicating that kidney and cardinal were on one chromosome in the F1 and ebony on the other.

(b) Crossing over between the loci k and e produces the kidney, ebony (128) and cardinal (138) offspring. Double crossovers are the triple mutants (6) and wild type (8). Altogether there are 128 + 138 + 6 + 8 = 280 crossovers between k and e: 280/4000 = 0:07 or 7% crossing over = 7 map units Crossovers between a and cd produced the single-crossover types kidney (97) and ebony, cardinal (89). Double crossovers again must be counted in this region. 97 + 89 + 6 + 8 = 200 crossovers between a and cd 200/4000 = 0:05 or 5% crossing over = 5 map units K 7 e a 5 cd

2.

Given the map segment: b 24.7 Cc= 0.85 DCO: 18 15 Find: SCOI & SCOII a 15.8 c

Answer: LUSCOI = SCOI + DCO/N x 100 24.7 = x + 18 + 15/1000 x 100 = 124/2= 62 LUSCOIi = SCOII + DCO/N x 100 15.8 = x + 18 + 15/1000 x 100 = 125/2 = 62.5 Last topic 1. Given the data: a. b. c. d. 12% A 30% T 15% G 20% C

Determine the % of other bases of each of the sample.

Answer: a. 12% A = 12% T 100-24=76/2 38% C = 38% G 30% T = 30% A 100-60=40/2 20% C = 20% G 15% G = 15% C 100-30=70/2 35%A = 35% T 20% C = 20% G 100-40=60/2 30%A = 30% T If each of the sample measures 3.5 cm, how many thiamine are presented in each sample?

b.

c.

d.

2.

3.5 cm (1/1x10-8 cm)(1bp/3.4)(2 bases/1 bp) = 2058823532.9 a. b. c. d. 2058823533 x .12 = 247058824 thiamine 2058823533 x .30 = 617647060 thiamine 2058823533 x .15 = 308823530 thiamine 2058823533 x .20 = 411764707 thiamine

2058823533